White paeony root whitening and anti-decline microneedle composition as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of skin care products, and particularly relates to a white peony root whitening and anti-aging product used in cooperation with a microneedle and a preparation method and application thereof. The white paeony root whitening and anti-decline microneedle composition is extracted from a traditional Chinese medicine white paeony root through a method of ethanol reflux extraction and C18 filler medium-pressure preparation, and is suitable for effectively penetrating through a skin epidermal layer through a microneedle body; the composition smoothly enters epidermis, dermis and subcutaneous tissues involved in the skin aging process, and meanwhile, the whitening, moisturizing and anti-aging effects are achieved at multiple levels and target spots.

Description

Technical Field

[0001] The present invention belongs to the technical field of skin care products, and specifically relates to a whitening and anti-aging product of white peony root used in combination with microneedles, and a preparation method and application thereof. Background Art

[0002] With the increase in the average life expectancy of the population and the aging of society, modern consumers are gradually becoming more aware of skin health management. Therefore, in-depth exploration of the mechanism of skin aging and the development of corresponding means to delay skin aging have become one of the research hotspots in the medical beauty industry. According to the product ingredients, the common whitening and anti-aging products on the current market have a single mechanism of action, and the anti-aging effect is often unsatisfactory. Based on the main action pathways involved in the skin aging process, it is of great significance to select different functional ingredients of natural origin, high safety, and excellent effects for scientific combination to develop new, synergistic, and efficient anti-aging products.

[0003] White peony root, Radix Paeoniae Alba., is the dried root of Paeonia lactiflora Pall., a perennial herb in the Ranunculaceae family. The root is enlarged and dark brown. It tastes sour and bitter, is slightly cold in nature, and enters the liver and spleen meridians. It has the effects of nourishing blood and regulating menstruation, restraining yin and stopping sweating, softening the liver and relieving pain, and calming liver yang. White peony root is often used to treat symptoms such as blood deficiency and chlorosis, irregular menstruation, spontaneous sweating, night sweats, flank pain, abdominal pain, cramps in the limbs, headache and dizziness. Modern research has found that the main active ingredients of white peony root, white peony polysaccharide and paeoniflorin, have a good inhibitory effect on the melanin-producing enzyme tyrosinase. In addition, white peony root also has anti-inflammatory, whitening, antioxidant, anti-aging effects, and has a good therapeutic effect on healthy skin management, and is widely used in clinical practice.

[0004] The application of functional skin care products begins on the surface of the skin, and needs to complete the transdermal absorption process, eventually enter the deep tissue of the epidermis, and accumulate to an effective concentration in the target cells at the target site to successfully play the target role. For various functional ingredients, the specific physiological structure of the skin protects the body from external damage while also limiting its transdermal delivery efficiency. Therefore, reducing the resistance of the stratum corneum, increasing skin permeability, allowing anti-aging functional ingredients to smoothly enter the epidermis, dermis and subcutaneous tissue involved in the skin aging process, and exerting anti-aging effects at multiple levels and targets is the key to solving the problem.

[0005] At present, the relatively mature microneedle technology can solve the problem of transdermal penetration of some effective ingredients. A certain number of microneedles are arranged in an array on a carrier, which can break through the barrier of the skin stratum corneum to form recoverable instant microchannels, so that the effective ingredients can be directly penetrated into the epidermis or dermis without causing pain or skin damage. However, the anti-aging products currently on the market generally use a single anti-aging ingredient. Due to the complexity of different anti-aging ingredients in terms of molecular size, solubility, stability, etc., different anti-aging ingredients interfere or react with each other, and it is impossible to realize the compound application of multiple anti-aging ingredients in microneedle technology. Summary of the invention

[0006] The purpose of the present invention is to provide a white peony root whitening and anti-aging microneedle composition, which is suitable for effectively penetrating the epidermis of the skin through the microneedle body, smoothly entering the epidermis, dermis and subcutaneous tissue involved in the skin aging process, and at the same time exerting whitening, moisturizing and anti-aging effects at multiple levels and targets.

[0007] The technical solution adopted by the present invention to solve its technical problem is:

[0008] A white peony root skin whitening and anti-aging microneedle composition, the white peony root skin whitening and anti-aging microneedle composition is prepared by the following method:

[0009] S1, reflux extraction: add 6-10 times the mass volume of 50-90% ethanol solution to the white peony root slices for reflux extraction 1-3 times, each time for 1-3 hours, filter and concentrate the filtrate;

[0010] S2, medium-pressure preparation with C18 filler: The concentrated filtrate obtained in S1 is subjected to column chromatography through an ODS medium-pressure preparation column, the filler is C18, and elution is performed in sequence using 6-15 retention volumes of methanol with a volume fraction of 20%, 40%, 60%, 80% and 100%; the 40% elution portion is collected, and methanol and water are recovered under reduced pressure to obtain the white peony essence extract;

[0011] S3. Add water to dilute the white peony root essence extract to a mass concentration of 12-15% to obtain the white peony root whitening and anti-aging microneedle composition.

[0012] Preferably, the microneedles include roller microneedles, radio frequency microneedles or microneedle patches.

[0013] Preferably, the microneedles are used on the face, neck or back. Before use, microneedles are first used to stimulate the epidermis and subcutaneous hair follicle area. The length of the microneedle needle used is 0.5-2.0 mm.

[0014] Preferably, the white peony root essence extract is added with water and diluted to a mass concentration of 13% to obtain the white peony root whitening and anti-aging microneedle composition.

[0015] Preferably, the white peony root whitening and anti-aging microneedle composition further comprises one or more of a preservative, a buffer, a disintegrant, an antioxidant, a suspending agent, a colorant and an excipient.

[0016] A method for preparing a whitening and anti-aging microneedle composition of white peony root, the method comprising the following steps:

[0017] S1, reflux extraction: add 6-10 times the mass volume of 50-90% ethanol solution to the white peony root slices for reflux extraction 1-3 times, each time for 1-3 hours, filter and concentrate the filtrate;

[0018] S2, medium-pressure preparation with C18 filler: The concentrated filtrate obtained in S1 is subjected to column chromatography through an ODS medium-pressure preparation column, the filler is C18, and elution is performed in sequence using 6-15 retention volumes of methanol with a volume fraction of 20%, 40%, 60%, 80% and 100%; the 40% elution portion is collected, and methanol and water are recovered under reduced pressure to obtain the white peony essence extract;

[0019] S3. Add water to dilute the white peony root essence extract to a mass concentration of 12-15% to obtain the white peony root whitening and anti-aging microneedle composition.

[0020] Preferably, the method comprises the following steps:

[0021] S1, reflux extraction: add 6-10 times the mass volume concentration of 70% ethanol solution to the white peony root slices and reflux extract 1-3 times, each time for 1-3h, filter and concentrate the filtrate;

[0022] S2, medium-pressure preparation with C18 filler: The concentrated filtrate obtained in S1 was passed through an ODS medium-pressure preparation column with C18 filler, and eluted with methanol at 8 retention volumes of 20%, 40%, 60%, 80% and 100% in sequence; the 40% elution fraction was collected, and methanol and water were recovered under reduced pressure to obtain the white peony essence extract;

[0023] S3. Add water to dilute the white peony root essence extract to a mass concentration of 12-15% to obtain the white peony root whitening and anti-aging microneedle composition.

[0024] Preferably, the white peony root essence extract is added with water and diluted to a mass concentration of 13% to obtain the white peony root whitening and anti-aging microneedle composition.

[0025] An application of the white peony root whitening and anti-aging microneedle composition of the present invention in the preparation of skin whitening, spot-lightening, moisturizing and anti-aging cosmetics.

[0026] The beneficial effects of the present invention are:

[0027] The white peony root whitening and anti-aging microneedle composition of the present invention is extracted from the traditional Chinese medicine white peony root by ethanol reflux extraction and C18 filler medium pressure preparation method. The white peony root whitening and anti-aging microneedle composition contains paeoniflorin, paeoniflorin, ethyl gallate and 1,2,3,4,6-pentagalloyl glucose.

[0028] The present invention preferably uses white peony functional ingredients, and purifies them through a reverse chromatographic column to obtain a white peony whitening and anti-aging microneedle composition. By giving the white peony whitening and anti-aging microneedle composition to photo-aged mice with microneedle treatment, it is unexpectedly found that the white peony whitening and anti-aging microneedle composition prepared by the present invention can effectively reduce superoxide dismutase (SOD), malondialdehyde (MDA) and inflammation, hair follicle dilation, etc. in skin tissue, and the effect of using with microneedles is better, which provides a new idea for whitening and anti-aging of traditional Chinese medicine. Compared with conventional white peony skin care products on the market, the white peony whitening and anti-aging microneedle composition prepared by the method of the present invention eliminates the inefficient components in white peony, and preserves and concentrates the important components that produce whitening and anti-aging effects in white peony essence. At the same time, the extraction method of the present invention has a higher yield.

[0029] According to the experimental results of the mouse photoaging model, simple microneedles, white peony whitening and anti-aging microneedle composition, and white peony whitening and anti-aging microneedle composition combined with microneedles can alleviate skin symptoms caused by ultraviolet irradiation, such as moisture, oil, elasticity, whiteness, SOD and MDA levels in skin tissue, etc. Compared with simple microneedles or simple white peony whitening and anti-aging microneedle composition, the white peony whitening and anti-aging microneedle composition prepared by the present invention has a better therapeutic effect when used with microneedles. The white peony whitening and anti-aging microneedle composition combined with microneedles showed a more obvious effect of improving skin dullness and aging. The arrangement of collagen fibers in the epidermis and subcutaneous layer of the mouse skin in the treatment group was closest to the blank group. In the drug-treated group, the white peony whitening and anti-aging microneedle composition combined with microneedles had the lowest degree of inflammatory cell infiltration in mice. Compared with simple microneedles and simple white peony root whitening and anti-aging microneedle combination, the white peony root whitening and anti-aging microneedle combination combined with microneedles can effectively penetrate the epidermis of the skin, smoothly enter the epidermis, dermis and subcutaneous tissue involved in the skin aging process, and exert whitening and anti-aging effects at multiple levels and targets.

[0030] Experiments have shown that the white peony root whitening and anti-aging microneedle composition of the present invention has the therapeutic effect of whitening and anti-aging when used with microneedles, can effectively alleviate water-oil imbalance, pigmentation, dullness, inflammatory infiltration, etc. of human skin tissue, and can be used to prepare whitening and anti-aging essence. BRIEF DESCRIPTION OF THE DRAWINGS

[0031] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative labor.

[0032] Figure 1 UPLC spectrum and characteristic peak identification of white peony root extract;

[0033] Figure 2 This is a trend chart of the change of the aging of the back skin of mice over time in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group in the animal experiment;

[0034] Figure 3 This is a statistical chart of the changes in moisture, oil content, whiteness and elasticity of the back skin of mice on the 30th day in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group in the animal experiment;

[0035] Figure 4 This is a statistical chart of the changes in MDA and SOD content in the back skin tissue of mice on the 30th day in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group in the animal experiment;

[0036] Figure 5 The staining pictures of the back skin tissue pathology sections of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group on the 30th day in the animal experiment;

[0037] Figure 6 Masson staining micrographs of the back skin tissue of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group on the 28th day;

[0038] Figure 7 These are Sirius red-stained micrographs of the back skin tissue of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence administration group on the 28th day. DETAILED DESCRIPTION

[0039] The technical solution of the present invention is further described in detail below through specific embodiments. It should be understood that the implementation of the present invention is not limited to the following embodiments, and any form of modification and / or change made to the present invention will fall within the protection scope of the present invention.

[0040] In the present invention, unless otherwise specified, all parts and percentages are by weight, and the equipment and raw materials used can be purchased from the market or are commonly used in the art. The methods in the following embodiments are all conventional methods in the art unless otherwise specified.

[0041] The reagents used in the following examples, unless otherwise specified, can be purchased from conventional biochemical reagent stores.

[0042] In the embodiment of the present invention, the photoaging model mice are preferably used as an example for verification, confirming the whitening and anti-aging activity of white peony root extract combined with microneedles.

[0043] The therapeutic effect of the drug of the present invention is preferably manifested in the following situations: reducing the inflammatory infiltration and SOD content of aged skin tissue, and increasing the SOD content and whiteness of aged skin tissue.

[0044] Example 1

[0045] A method for preparing a whitening and anti-aging microneedle composition of white peony root, comprising the following specific steps:

[0046] S1, reflux extraction: select white peony root slices, add 10 times the mass volume concentration of 70% ethanol solution, reflux extraction 3 times, each time for 2 hours, filter and concentrate the filtrate to obtain white peony root methanol extract (MPRA);

[0047] S2, C18 medium-pressure preparation: The concentrated filtrate obtained from S1 was chromatographed on an ODS medium-pressure preparative column with a detection wavelength of 254 nm and C18 filler. Eight retention volumes of methanol with volume fractions of 20%, 40%, 60%, 80% and 100% were used for elution.

[0048] The 40% elution fraction was collected, and methanol and water were recovered under reduced pressure to obtain the white peony root extract (MP-40).

[0049] The UPLC spectrum and characteristic peak identification of the white peony extract obtained are as follows: Figure 1 As shown. The essence contains paeoniflorin, paeoniflorin, ethyl gallate and 1,2,3,4,6-pentagalloylglucose. The yield of white peony methanol extract (MPRA) in this process is 13.6%, and the yield of white peony essence extract (MP-40) is 3.45%. Accurately weigh the white peony essence extract, add deionized water to obtain a mass fraction of 13%, and the white peony whitening and anti-aging microneedle composition described in the present invention can be obtained.

[0050] Example 2: Verification of the efficacy of whitening and anti-aging by combining white peony root extract (MP-40) with microneedle therapy

[0051] 1. Drug preparation

[0052] White peony root extract: Accurately weigh the white peony root extract, dissolve it in deionized water to prepare 13% white peony root extract, and apply 1.5 ml to each mouse.

[0053] 2. Modeling and drug administration

[0054] 2.1 Mouse photoaging model

[0055] 25 Balb / c male mice, weighing 17-22g, were randomly divided into 5 groups, 5 mice in each group. Subsequent experiments were conducted after one week of adaptation to the environment. The experimental groups were divided into a blank group, a photoaging model group, a photoaging model plus microneedle group, a photoaging model plus white peony essence group, and a photoaging model plus microneedle combined with white peony essence group. Before the experiment, the back of each group of mice was depilated 3cm*3cm, and the hair was shaved once every two days to prepare for photoaging.

[0056] Before modeling, prepare two 40W UVA lamps (lamp wavelength 320-400nm, peak 365nm) and two 40W UVB lamps (lamp wavelength 290-320nm, peak 297nm), and install the four lamps in parallel in a homemade simulated sun box. The blank group mice were exposed to normal sunlight for 40 minutes per day, and the photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group were exposed to UVA+UVB in a simulated irradiation box for 40 minutes per day, once a day for 28 days. The irradiation dose was calculated according to the formula: UV dose = UV radiation intensity × time. The cumulative UVA irradiation intensity was 261.79 J / cm 2 The cumulative UVB radiation intensity is 6.54 J / cm 2 , reaching the ultraviolet radiation intensity recorded in the literature. The blank group, model group, and model plus microneedle group were smeared with 1.5ml of normal saline, the photoaging model plus white peony essence group was smeared with 1.5ml of 13% white peony whitening and anti-aging microneedle composition, and the photoaging model plus microneedle combined with white peony essence group was given 0.2mm roller microneedle repeatedly several times and then smeared with 1.5ml of 13% white peony whitening and anti-aging microneedle composition. Apply once a day for 28 days. After the last administration, the mice were anesthetized and the mouse skin tissue was preserved in 10% formalin.

[0057] 3. Changes in mouse skin condition

[0058] Continue to pay attention to the skin changes of mice during UV light exposure. According to the Glogau classification of skin photoaging, observe whether the back skin of mice has symptoms such as dryness, thickening, wrinkles, and redness. Take pictures every week to record the skin appearance and judge the changes in classification. The Glogau classification of skin photoaging is mainly divided into four types, namely: Type I: "No wrinkles", showing slight pigmentation, no keratinization, and few wrinkles. Type II: "Wrinkles in motion", showing that keratinization is palpable but unclear, capillary expansion, and slight dullness. Type III: "Wrinkles in rest", showing obvious pigment abnormalities, obvious capillary expansion, obvious keratinization, and visible wrinkles in rest. Type IV: "Only wrinkles", showing severe dullness, obvious wrinkles, obvious keratinization, and obvious capillary expansion. In the animal experiment, the aging trend of the back skin of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group over time is shown in the results. Figure 2 .

[0059] like Figure 2 As shown in the figure, on day 0, before the photoaging modeling began, the skin condition of mice in each group was normal, showing delicate and smooth skin with white skin color. The blank group did not receive UV light irradiation intervention. During the 28-day trial, the skin continued to be in a normal state, and it can be divided into type I according to the Glogau classification standard for skin photoaging. On day 7, the photoaging model group showed photoaging phenomena such as keratinization, roughness, redness of the epidermis, and reduced elasticity, indicating that the modeling was successful. According to the Glogau classification standard for skin photoaging, it can be divided into type II. The improvement effect of the other treatment groups was not obvious. On day 28, the photoaging model group and each treatment group continued to be irradiated with UV light and treated accordingly. Among them, the skin aging of the model group continued to worsen, and was divided into type IV according to the Glogau classification standard for photoaging. The specific manifestations of the mouse skin were deepening of dynamic and static wrinkles, severe keratinization, obvious roughness, skin redness, obvious vascular dilation, and reduced elasticity; the symptoms of each treatment group continued to alleviate over time, with reduced roughness, fewer wrinkles, and less redness. Among them, the photoaging model plus microneedle combined with white peony essence administration group had the best effect, which was classified as type I. Therefore, the roller microneedle combined with white peony whitening and anti-aging microneedle combination has a good therapeutic effect on skin whitening and anti-aging.

[0060] 4. Changes in skin index of mice in each group

[0061] The skin condition of the mouse back was tested every day using a skin tester. Figure 3As shown, compared with the blank group, the whiteness, elasticity, moisture and oil content of the skin of mice in the photoaging model group were significantly decreased, indicating that the photoaging model group was successfully modeled; compared with the photoaging model group, the whiteness, elasticity, moisture and oil content of the skin of mice in the photoaging model plus microneedle group, the photoaging model plus white peony essence group, and the photoaging model plus microneedle combined with white peony essence group showed different degrees of improvement, among which the improvement effect of the skin of mice in the photoaging model plus microneedle combined with white peony essence group was the best.

[0062] 5. Changes of MDA and SOD contents in skin tissues of mice in each group

[0063] Long-term ultraviolet radiation will cause excessive ROS production in the skin. The skin is unable to metabolize the large amount of accumulated ROS, causing an imbalance in the oxidation and antioxidant systems, an increase in the content of lipid peroxidation products MDA, and a decrease in the level of antioxidant enzyme SOD. Therefore, testing the levels of SOD and MDA in the skin can reflect the skin's ability to resist oxidative stress and anti-aging.

[0064] The statistical results of the changes in MDA and SOD contents in the back skin tissue of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence administration group on the 30th day in the animal experiment are as follows Figure 4 As shown in the figure, compared with the blank group, the MDA content in the skin tissue of mice in the photoaging model group increased significantly, while the SOD content decreased significantly, indicating that the photoaging model was successfully established. Compared with the photoaging model group, the MDA and SOD contents in the skin tissue of mice in the photoaging model plus microneedle group, the photoaging model plus white peony essence group, and the photoaging model plus microneedle combined with white peony essence administration group were reversed to varying degrees, among which the improvement effect of MDA and SOD in the skin tissue of mice in the photoaging model plus microneedle combined with white peony essence administration group was the most significant. This shows that the microneedle combined with white peony whitening and anti-aging microneedle composition has a good anti-aging effect.

[0065] 5. Histopathological study of animal skin inflammatory infiltration

[0066] Mouse skin tissue samples were fixed with formalin for one week, then removed and rinsed with running water to remove excess formalin on the tissue surface. A razor blade was used to cut a volume of about 1 cm 3The skin tissue was rinsed continuously with running water for more than 2 hours. All samples were dehydrated using a fully automatic dehydrator. Then, an embedding machine was used to embed the tissue with paraffin. After cooling to room temperature, all samples were sliced ​​with a thickness of about 4 μm, and then sliced ​​with glass slides. The paraffin sections were placed in an oven and melted at 60°C for more than 2 hours. After dewaxing and hydration, H&E, Masson, and Sirius red staining were performed. After completion, 1 to 2 drops of neutral gum were dropped on a glass slide, which was sealed with a coverslip and placed under an optical microscope to observe the inflammatory infiltration of the animal's skin tissue.

[0067] The test results are as follows Figure 5 The following are HE-stained micrographs of the back skin tissue of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group on day 28. The HE-stained images show that the epidermis of the back skin of mice in the blank group has a clear structure, with about 1-2 layers of keratinocytes, and the cells are arranged neatly. The dermis contains abundant fibrous tissue, and the fiber thickness and distribution are uniform, without obvious breakage or disordered arrangement. Compared with the blank group, the epidermis of the photoaging model group was significantly thickened, consisting of about 8-12 layers of keratinocytes, with unclear intercellular stratification, significantly reduced dermal fibers, loose arrangement, and more inflammatory cells infiltrated in the whole dermis; compared with the model group, the epidermis thickness of the photoaging model plus microneedle group decreased, consisting of about 5-8 layers of keratinocytes, the dermal fibers were degenerated and arranged in disorder, and there were fewer inflammatory cells infiltrated in the superficial and middle layers of the dermis, and the damage was lighter than that in the model group; compared with the model group, the epidermis thickness of the photoaging model plus white peony essence group decreased, consisting of about 5-9 layers of keratinocytes, the dermal fibers were degenerated and arranged in disorder, and there were fewer inflammatory cells infiltrated in the superficial layer of the dermis, and the damage was lighter than that in the model group; the epidermis thickness of the skin of mice in the photoaging model plus microneedle combined with white peony essence group was significantly thinner than that in the model group, consisting of about 3-5 layers of keratinocytes, the dermal fibers were densely arranged than those in groups B, C, and D, and there were scattered inflammatory cells infiltration in the superficial layer of the dermis, with the mildest degree of damage.

[0068] like Figure 6The following are Masson staining micrographs of the back skin tissue of mice in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence group on the 28th day. The dermal collagen fibers in the blank group were wavy, tightly arranged, evenly distributed, and orderly; compared with the blank group, the dermal collagen fibers in the photoaging model group were degraded, loosened, denatured, broken, and disordered, and the content decreased; compared with the photoaging model group, the photoaging model plus microneedle group, the photoaging model plus white peony essence group, and the photoaging model plus microneedle combined with white peony essence group had dense collagen fibers in the dermis and increased collagen fiber content. Among them, the photoaging model plus microneedle combined with white peony essence group had the most dermal collagen fibers, which were densely arranged, neatly distributed, and evenly distributed.

[0069] like Figure 7 As shown, the Sirius red staining micrographs of the back skin tissue of mice on the 28th day in the blank group, photoaging model group, photoaging model plus microneedle group, photoaging model plus white peony essence group, and photoaging model plus microneedle combined with white peony essence administration group. Under an optical microscope, the collagen fibers in the mouse skin tissue sections were red, the cell nuclei were brown to black, and the muscle fibers were yellow. The cells in the blank group were neatly arranged and the tissue morphology was normal. The thickness of the epidermis in the photoaging model group increased significantly, the cell organization was disordered, and the main collagen fibers and muscle fibers were significantly reduced. Compared with the photoaging model group, the photoaging model plus microneedle group, the photoaging model plus white peony essence group, and the photoaging model plus microneedle combined with white peony essence administration group had more orderly cell arrangement and increased collagen fiber content. Among them, the photoaging model plus microneedle combined with white peony essence administration group had the best effect, proving that the microneedle combined with white peony whitening and anti-aging microneedle composition has a good whitening and repairing effect.

[0070] In summary, the white peony root whitening and anti-aging microneedle composition described in the present invention can be used in combination with microneedles to break the skin, allowing the white peony root essence to reach the dermal cell layer directly, achieving a better whitening and anti-aging effect than using the white peony root essence alone.

[0071] In this specification, each embodiment is described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the embodiments can be referred to each other. For the device disclosed in the embodiment, since it corresponds to the method disclosed in the embodiment, the description is relatively simple, and the relevant parts can be referred to the method part.

[0072] The above is a detailed introduction to a white peony root anti-aging microneedle composition, a preparation method and an application thereof provided by the present invention. Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core idea. It should be pointed out that for ordinary technicians in this technical field, without departing from the principles of the present invention, the present invention can also be improved and modified in a number of ways, and these improvements and modifications also fall within the scope of protection of the claims of the present invention.

Claims

1. A whitening and anti-aging microneedle composition of white peony root, characterized in that The whitening and anti-aging microneedle composition of white peony root is prepared by the following method: S1, reflux extraction: add 6-10 times the mass volume of 50-90% ethanol solution to the white peony root slices for reflux extraction 1-3 times, each time for 1-3 hours, filter and concentrate the filtrate; S2, C18 medium pressure preparation: The concentrated filtrate obtained in S1 was subjected to column chromatography through an ODS medium pressure preparation column, the filler was C18, and eluted with 6-15 retention volumes of methanol with a volume fraction of 20%, 40%, 60%, 80% and 100% in sequence; Collect 40% of the eluted fraction, recover methanol and water under reduced pressure, and obtain white peony root extract; S3. Add water to dilute the white peony root essence extract to a mass concentration of 12-15% to obtain the white peony root whitening and anti-aging microneedle composition.

2. The whitening and anti-aging microneedle composition of white peony root according to claim 1, characterized in that: The microneedles include roller microneedles, radio frequency microneedles or microneedle patches.

3. The whitening and anti-aging microneedle composition of white peony root according to claim 1, characterized in that: The microneedles are used on the face, neck or back. Before use, they need to be used to stimulate the epidermis and subcutaneous hair follicle area. The length of the microneedle needle used is 0.5-2.0 mm.

4. The whitening and anti-aging microneedle composition of white peony root according to claim 1, characterized in that: The white peony root essence extract is added with water and diluted to a mass concentration of 13% to obtain the white peony root whitening and anti-aging microneedle composition.

5. The whitening and anti-aging microneedle composition of white peony root according to claim 1, characterized in that: The white peony root whitening and anti-aging microneedle composition also includes one or more of a preservative, a buffer, a disintegrant, an antioxidant, a suspending agent, a colorant and an excipient.

6. A method for preparing a whitening and anti-aging microneedle composition of white peony root, characterized in that The method comprises the following steps: S1, reflux extraction: add 6-10 times the mass volume of 50-90% ethanol solution to the white peony root slices for reflux extraction 1-3 times, each time for 1-3 hours, filter and concentrate the filtrate; S2, C18 medium pressure preparation: The concentrated filtrate obtained in S1 was subjected to column chromatography through an ODS medium pressure preparation column, the filler was C18, and eluted with 6-15 retention volumes of methanol with a volume fraction of 20%, 40%, 60%, 80% and 100% in sequence; Collect 40% of the eluted fraction, recover methanol and water under reduced pressure, and obtain white peony root extract; S3. Add water to dilute the white peony root essence extract to a mass concentration of 12-15% to obtain the white peony root whitening and anti-aging microneedle composition.

7. The preparation method according to claim 6, characterized in that The method comprises the following steps: S1, reflux extraction: add 6-10 times the mass volume concentration of 70% ethanol solution to the white peony root slices and reflux extract 1-3 times, each time for 1-3h, filter and concentrate the filtrate; S2, C18 medium pressure preparation: The concentrated filtrate obtained in S1 was subjected to column chromatography using an ODS medium pressure preparation column, the filler being C18, and eluted using 8 retention volumes of methanol with a volume fraction of 20%, 40%, 60%, 80% and 100% in sequence; Collect 40% of the eluted fraction, recover methanol and water under reduced pressure, and obtain white peony root extract; S3. Add water to dilute the white peony root essence extract to a mass concentration of 12-15% to obtain the white peony root whitening and anti-aging microneedle composition.

8. The preparation method according to claim 6, characterized in that: The white peony root essence extract is added with water and diluted to a mass concentration of 13% to obtain the white peony root whitening and anti-aging microneedle composition.

9. Application of a white peony root whitening and anti-aging microneedle composition in the preparation of skin whitening, spot-lightening, moisturizing and anti-aging cosmetics.

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