Preparation method of embedded metasurface-micro-fluidic chip

Abstract

The invention belongs to the technical field of microfluidic devices and optical metasurfaces. The invention provides a preparation method of an embedded metasurface-micro-fluidic chip. According to the embodiment of the invention, the metalized nano array is embedded into the micro-fluidic chip, and the array is fixed and uniform, so that the interference of the flow of the metal nano particle dispersion liquid in the conventional SERS-micro-fluidic technology on the target detection object can be eliminated, and the unmarked SERS-micro-fluidic technology is realized; and uniform hot spots can be generated to enhance Raman signals of cells in the microfluid. The prepared embedded metasurface-micro-fluidic chip has good biological compatibility, and the Raman spectrum intensity of biological cells can be remarkably enhanced; moreover, the optical power required for exciting the Raman of the biological cells can be effectively reduced, and the damage to the biological cells to be detected caused by too high exciting optical power is avoided.

Description

Technical Field

[0001] The disclosed embodiments relate to the technical field of microfluidic devices and optical metasurfaces, and in particular to a method for preparing an embedded metasurface-microfluidic chip. Background Art

[0002] Optical metasurfaces, that is, two-dimensional arrangements of rationally designed subwavelength scatterers, introduce mutations in optical properties through the interaction between light waves and the unit structures and functional arrangements of these scatterers, achieve the regulation and manipulation of phase, polarization and amplitude, and reduce the dependence on propagation effects. The scatterer units of the metasurface are usually composed of geometric structures or materials with spatially varying characteristics, and can shape the wavefront into any shape according to scientific design. Optical metasurfaces have attracted widespread attention from domestic and foreign researchers due to their amazing ability to control light fields, and are considered to be a new paradigm for light field control and even the entire electromagnetic wave engineering. At present, metasurfaces have been applied to many fields such as superlenses, chiral molecule detection, super-resolution imaging, spectral detection, face recognition, optical camouflage, microfluidic chips, etc. Among them, the relevant research on the combination of metasurfaces and microfluidic chips focuses on microfluidic manipulation and microfluidic structural color. Considering the important application of microfluidic chips in biological detection, the powerful ability of optical metasurfaces in light field control, and the portability and compatibility of devices, the study of microfluidic technology based on optical metasurfaces will open up a new platform for biological detection technology.

[0003] Microfluidics refers to the technology that integrates the reaction and detection functions of traditional experiments on microfluidic chips, thereby significantly reducing the space required for experiments, and is applied to the detection of biology, chemistry, physics, medicine and other aspects related to fluid science. Microfluids are liquids carried by microfluidic chips. They have a high surface area-to-volume ratio and usually have different physical and chemical properties from macroscopic fluids. Therefore, they have become a hot spot in the scientific frontier at home and abroad. The vigorous development of micro-nano processing technology in recent years has greatly promoted the development and progress of microfluidics and related microscale characterization and detection technologies, and microfluidic chips with different structures have also emerged in an endless stream. Surface plasmon microfluidic chips integrate surface plasmon nanostructures into microfluidic chips, bringing the advantages of surface plasmon devices such as small size, high sensitivity to environmental changes, electromagnetic field enhancement and spectral enhancement capabilities into the field of microfluidics, and forming a new microfluidic detection technology-surface enhanced Raman spectroscopy (SERS)-microfluidics technology. SERS technology refers to the localized surface plasmon resonance (LSPR) effect generated when incident light interacts with surface plasmon nanostructures such as gold nanorods, silver nanocones, copper nanospheres, etc., which produces a significant electric field enhancement effect in the subwavelength scale, thereby achieving Raman spectroscopy enhancement. Raman spectroscopy can reflect the chemical composition information of the substance to be tested. It is a label-free material identification technology and is called the "fingerprint" spectrum of the substance. SERS-microfluidic technology encompasses the advantages of SERS technology and microfluidic technology, and studies have proven the high compatibility of SERS technology and microfluidic technology. The development of SERS-microfluidic technology is of great significance for the changes in chemical composition of microfluidic micro-regions.

[0004] However, current SERS-microfluidics usually uses a method in which metal nanoparticles dispersed in a liquid are mixed with the microfluid to be tested. This method introduces metal nanoparticles into the microfluidic channel, which weakens the label-free advantage of this technology. The movement of metal nanoparticles introduced into the microfluidic channel and the difference in movement between the metal nanoparticles and the microfluid to be tested result in low uniformity of hot spots, poor near-field confinement, and low detection sensitivity provided by this method. So how to achieve label-free SERS-microfluidics and how to eliminate the interference of the movement of metal nanoparticles in the microfluid on the target detection object are problems that technicians in this field urgently need to solve.

[0005] Therefore, it is necessary to improve one or more problems existing in the above-mentioned related technical solutions.

[0006] It should be noted that this section is intended to provide background or context for the technical solutions of the present disclosure stated in the claims. The description herein is not admitted to be prior art by virtue of being included in this section. Summary of the invention

[0007] In order to avoid the shortcomings of the prior art, the present application provides a method for preparing an embedded supersurface-microfluidic chip to solve the problem in the prior art that the movement of metal nanoparticles in the microfluidic will interfere with the target detection object.

[0008] According to a first aspect of an embodiment of the present disclosure, a method for preparing an embedded supersurface-microfluidic chip is provided, the method comprising: Placing a first glass sheet in a container, injecting deionized water into the container until the first glass sheet is submerged, and injecting a PS ball layer onto the liquid surface of the deionized water in the container using a micro-injector at a preset volume rate; Dropping a plurality of drops of sodium dodecyl sulfate aqueous solution at a plurality of positions along the side wall of the container to make the PS (polystyrene) ball layer densely packed and arranged, then releasing the deionized water in the container to make the PS ball layer drop along with the liquid level until the PS ball layer is deposited on the surface of the first glass sheet; Mixing equal volumes of silver ammonia solution and potassium sodium tartrate solution to form a mixed solution, spraying the mixed solution on the surface of the PS ball layer through a sprayer by a chemical spraying method, and standing until a silver single substance layer is generated on the surface of the PS ball layer to obtain a super surface array; Inverting the first glass sheet onto a second glass sheet carrying a microfluidic channel to embed the metasurface array into the microfluidic channel; wherein the first glass sheet is smaller than the second glass sheet; The first needle and the second needle are fixed to the edge of the first glass sheet and the second glass sheet using ultraviolet curing glue, and the ultraviolet curing glue is irradiated with ultraviolet light to cure it, so as to obtain an embedded metasurface-microfluidic chip; wherein the first needle is the inlet of the embedded metasurface-microfluidic chip, the second needle is the outlet of the embedded metasurface-microfluidic chip, and the first needle and the second needle are both connected to the microfluidic channel.

[0009] Furthermore, a water control valve is provided on the container, through which the deionized water is injected into or released from the container, and the diameter of the water control valve is 2-5 mm.

[0010] Furthermore, the diameter of the PS sphere layer is less than or equal to 300 nm, and the volume velocity of the injection of the PS sphere layer is 10-50 μL / min.

[0011] Furthermore, the mass fraction of the sodium dodecyl sulfate aqueous solution is 1%-5%, and the number of drops of the sodium dodecyl sulfate aqueous solution added is 1-3 drops.

[0012] Furthermore, the concentration of the silver ammonia solution is 0.01 g / ml, and the concentration of the potassium sodium tartrate solution is 0.05 g / ml; wherein the silver ammonia solution is composed of AgNO 3 The solution is made with ammonia.

[0013] Furthermore, in the chemical spraying method, the spraying direction of the sprayer is perpendicular to the plane of the PS ball layer, and the distance between the sprayer and the plane of the PS ball layer is greater than or equal to 10 cm.

[0014] Furthermore, the sprayer can be applied 2-4 times.

[0015] According to a second aspect of an embodiment of the present disclosure, there is provided an embedded supersurface-microfluidic chip, which is made by any of the preparation methods described above.

[0016] The technical solution provided by the embodiments of the present disclosure may have the following beneficial effects: In the embodiments disclosed herein, through the above-mentioned embedded super surface-microfluidic chip and its preparation method, on the one hand, the metallized nano array is embedded in the microfluidic chip, and the array is fixed and uniform, which can not only eliminate the interference of the flow of the metal nanoparticle dispersion in the conventional SERS-microfluidic technology on the target detection object, realize the label-free SERS-microfluidic technology, but also can generate uniform hot spots to enhance the Raman signal of the cells in the microfluid. On the other hand, the prepared embedded super surface-microfluidic chip has good biocompatibility, can significantly enhance the Raman spectrum intensity of biological cells; and can effectively reduce the light power required to excite the Raman of biological cells, avoiding the damage to the biological cells to be tested caused by excessive excitation light power. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The accompanying drawings herein are incorporated into the specification and constitute a part of the specification, illustrate embodiments consistent with the present disclosure, and together with the specification are used to explain the principles of the present disclosure. Obviously, the accompanying drawings described below are only some embodiments of the present disclosure, and for ordinary technicians in this field, other accompanying drawings can be obtained based on these accompanying drawings without creative work.

[0018] Figure 1 A diagram showing the steps of a method for preparing an embedded supersurface-microfluidic chip in an exemplary embodiment of the present disclosure; Figure 2 A schematic diagram showing a process of preparing an embedded supersurface-microfluidic chip in an exemplary embodiment of the present disclosure; Figure 3 A geometric topography image of an embedded metasurface recorded by a scanning electron microscope in an exemplary embodiment of the present disclosure is shown; Figure 4The Raman spectra of mouse blood cell microfluids recorded by the Raman spectrometer in the exemplary embodiment of the present disclosure under the condition of irradiating the metasurface-microfluidic chip with radially polarized vector light field are shown; wherein (a) is the Raman spectrum without adding glucose; (b) is the Raman spectrum with adding glucose at a concentration of 10 -1 M glucose solution; (c) Raman spectrum of the solution with 10 -2 M glucose solution; (d) Raman spectrum of the glucose solution with 10 -3 Raman spectrum of glucose solution of 100 M. DETAILED DESCRIPTION

[0019] Example embodiments will now be described more fully with reference to the accompanying drawings. However, example embodiments can be implemented in a variety of forms and should not be construed as limited to the examples set forth herein; rather, these embodiments are provided so that the disclosure will be more comprehensive and complete and to fully convey the concepts of the example embodiments to those skilled in the art. The described features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

[0020] In addition, the accompanying drawings are only schematic illustrations of the embodiments of the present disclosure and are not necessarily drawn to scale. The same reference numerals in the figures represent the same or similar parts, and their repeated descriptions will be omitted. Some of the block diagrams shown in the accompanying drawings are functional entities and do not necessarily correspond to physically or logically independent entities.

[0021] In this exemplary embodiment, a method for preparing an embedded super-surface-microfluidic chip is first provided. Figure 1 As shown in , the method for preparing the embedded supersurface-microfluidic chip may include: steps S101 to S105.

[0022] Step S101: placing a first glass sheet in a container, injecting deionized water into the container until the first glass sheet is submerged, and injecting a PS ball layer onto the liquid surface of the deionized water in the container using a micro-injector at a preset volume rate; Step S102: dripping a plurality of drops of sodium dodecyl sulfate aqueous solution at a plurality of positions along the side wall of the container to make the PS ball layer densely packed, and then releasing the deionized water in the container to make the PS ball layer drop along with the liquid level until the PS ball layer is deposited on the surface of the first glass sheet; Step S103: mixing equal volumes of silver ammonia solution and potassium sodium tartrate solution to form a mixed solution, spraying the mixed solution on the surface of the PS ball layer through a sprayer by a chemical spraying method, and standing until a silver single substance layer is formed on the surface of the PS ball layer to obtain a super surface array; Step S104: Inverting the first glass sheet onto a second glass sheet carrying a microfluidic channel to embed the metasurface array into the microfluidic channel; wherein the first glass sheet is smaller in size than the second glass sheet; Step S105: Use ultraviolet curing glue to fix the first needle and the second needle on the edge of the first glass sheet and the second glass sheet, and use ultraviolet light to irradiate the ultraviolet curing glue to cure it, so as to obtain an embedded metasurface-microfluidic chip; wherein, the first needle is the inlet of the embedded metasurface-microfluidic chip, the second needle is the outlet of the embedded metasurface-microfluidic chip, and the first needle and the second needle are both connected to the microfluidic channel.

[0023] Through the above-mentioned embedded super surface-microfluidic chip and its preparation method, on the one hand, the metallized nano array is embedded in the microfluidic chip, and the array is fixed and uniform, which can not only eliminate the interference of the flow of metal nanoparticle dispersion in conventional SERS-microfluidic technology on the target detection object, realize label-free SERS-microfluidic technology, but also generate uniform hot spots to enhance the Raman signal of cells in the microfluid. On the other hand, the prepared embedded super surface-microfluidic chip has good biocompatibility and can significantly enhance the Raman spectrum intensity of biological cells; and can effectively reduce the light power required to excite the Raman of biological cells, avoiding damage to the biological cells to be tested caused by excessive excitation light power.

[0024] Next, we will refer to Figure 1 to Figure 2 The above-mentioned embedded supersurface-microfluidic chip and each step of the preparation method thereof in this example embodiment are described in more detail.

[0025] In step S101, Figure 2 As shown, a first glass sheet is placed on a shelf in a container, wherein a water control valve is provided in the middle of the bottom of the container, deionized water is slowly injected into the container until the glass sheet is submerged, and a PS ball layer is injected onto the deionized water surface in the container at a certain speed using a micro-injector. The micro-injector is connected to a hose with a needle, and the needle is fixed by a clamp so that the needle outlet plane is parallel to the deionized water surface, and the height of the needle outlet is set so that the droplets drip out of the needle just contact the deionized water surface in the container.

[0026] In addition, the glass sheets, racks, containers, syringes, etc. involved in this application are all cleaned with alcohol and deionized water in advance.

[0027] In step S102, Figure 2As shown, one drop of sodium dodecyl sulfate aqueous solution is dripped into each of the four directions along the side wall of the container, the surface tension of the liquid is changed to make the PS ball layer densely packed and arranged, the water control valve is opened to let the liquid in the container flow out, so that the liquid level drops, and the PS ball layer on the liquid surface drops with the drop of the liquid level until the PS ball layer is deposited on the surface of the first glass sheet.

[0028] In step S103, Figure 2 As shown, a freshly prepared silver ammonia solution (AgNO 3 The solution was mixed with an appropriate amount of ammonia water and potassium sodium tartrate solution in equal volumes, and the mixture was sprayed on the surface of the PS ball layer using a spray bottle and allowed to stand until the mixture was completely reacted to form a silver single layer to obtain a metasurface array.

[0029] In step S104, Figure 2 As shown, a first glass sheet with a PS ball layer deposited on its surface is covered on the surface of a second glass sheet equipped with a microfluidic channel; wherein the length and width of the first glass sheet are smaller than those of the second glass sheet, and one side of the PS ball layer faces the side of the microfluidic channel, and the metasurface array is embedded in the microfluidic channel.

[0030] In step S105, Figure 2 As shown, the UV curing glue is evenly applied to the edge of the first glass sheet and the edge of the needle tube at the interface between the first needle, the second needle and the second glass sheet, and then the UV curing glue is irradiated with UV light to solidify it, thereby realizing the packaging of the embedded supersurface-microfluidic chip, wherein the first needle and the second needle are the inlet and outlet of the chip respectively.

[0031] In one embodiment, the diameter of the water control valve is 2-5 mm.

[0032] In one embodiment, the diameter of the PS sphere layer is less than or equal to 300 nm, and the volume rate of the PS sphere layer injection is 10-50 μL / min.

[0033] In one embodiment, the mass fraction of the sodium dodecyl sulfate aqueous solution is 1%-5%, and the number of drops of the sodium dodecyl sulfate aqueous solution dropped is 1-3 drops.

[0034] In one embodiment, the concentration of the silver ammonia solution is 0.01 g / ml, and the concentration of the potassium sodium tartrate solution is 0.05 g / ml; wherein the silver ammonia solution is composed of AgNO 3 The solution is made with ammonia.

[0035] In one embodiment, in the chemical spraying method, the spraying direction of the sprayer is perpendicular to the plane of the PS ball layer, and the distance between the sprayer and the plane of the PS ball layer is greater than or equal to 10 cm.

[0036] In one embodiment, the spraying number of the sprayer is 2-4 times.

[0037] This exemplary embodiment also provides an embedded supersurface-microfluidic chip, which is made by the above-mentioned embedded supersurface-microfluidic chip preparation method.

[0038] The application method of the embedded metasurface-microfluidic chip is as follows: the embedded metasurface-microfluidic chip is used to enhance the Raman scattering intensity of biological microfluids, wherein the embedded metasurface is composed of an array of metallized spheres with a diameter not greater than 300 nm; low-power spatial radially polarized vector light is used to irradiate the embedded metasurface-microfluidic chip, that is, low-power spatial radially polarized vector light is used as excitation light, and the Raman spectrum intensity of the biological microfluids is enhanced through the interaction between the spatial radially polarized vector light and the embedded metasurface, while avoiding damage to biological cells caused by high-power excitation light, and further applied to monitoring the response of biological cells to environmental changes such as changes in environmental glucose concentration.

[0039] Specifically, low-power spatial radially polarized vector light is used to irradiate the above-mentioned microfluidic chip (i.e., embedded metasurface-microfluidic chip), and the biological cell microfluid to be tested is passed into the chip, wherein the spatial radially polarized vector light is converted from linearly polarized light, that is, spatial radially polarized vector light with a very narrow wavelength linewidth is generated after passing through a linearly polarized solid laser, a narrow-band filter, a polarizer, and a zero-order vortex half-wave plate as excitation light for Raman spectroscopy detection, and the excitation light is irradiated onto the microfluidic chip through a dichroic mirror and a microscope objective, and the Raman signal of the microfluid is enhanced by the silver-coated nanosphere array in the chip, and then collected by the microscope objective, and reaches the Raman spectrometer through a reflector, a long-wave pass edge filter, a convex lens, and a multimode optical fiber.

[0040] The biological cell microfluid to be tested is injected into the microfluidic chip by pushing the micro-syringe through the electric displacement stage, wherein the micro-syringe is connected to the inlet end of the above chip through a transparent silicone hose, and the outlet end of the above chip is led to the waste liquid collection vessel by another transparent silicone hose. The Raman signal is collected by a long working distance microscope objective, passes through a dichroic mirror, and then passes through a long-wave passband edge filter and a convex lens to focus on the end face of a large core diameter multimode optical fiber, and is transmitted into the Raman spectrometer by a multimode optical fiber, thereby realizing the collection of the Raman spectrum of the microfluid.

[0041] In one embodiment, low-power spatial radially polarized vector light is used to illuminate the embedded metasurface-microfluidic chip to avoid biological cell damage caused by high power. The wavelength of the spatial radially polarized vector light is 532 nm, the power is no more than 25 mW, and the light field mode is continuous light.

[0042] In one embodiment, spatial radially polarized vector light is used to excite the Raman signal of the microfluid to be measured, and is focused onto the above-mentioned microfluidic chip through a microscope objective with a working distance greater than 1 mm, so that the focal plane of the excitation light can pass through the upper glass sheet to reach the super-surface layer, wherein the thickness of the upper glass sheet is less than 1 mm.

[0043] In one embodiment, before measuring the Raman spectrum of the biological cell microfluid, the super surface-microfluidic chip and the micro syringe need to be cleaned and sterilized. First, the micro syringe, the transparent silicone hose, and the super surface-microfluidic chip are connected, and 75% of the alcohol is drawn with a micro syringe, and passed into the prepared super surface-microfluidic chip at a speed of 1 μL / min. After the alcohol in the micro syringe completely flows out of the microfluidic chip into the waste liquid collection vessel, the full scale of deionized water is drawn with a micro syringe, and after the deionized water in the micro syringe completely flows out of the microfluidic chip into the waste liquid collection vessel, the connected micro syringe, the transparent silicone hose, and the super surface-microfluidic chip are placed in an operating room with an ultraviolet disinfection lamp for sterilization and disinfection, and the disinfection time is not less than 2 hours.

[0044] In a specific embodiment, the surface morphology of the prepared metasurface array was imaged, and the scanning electron microscope imaging results were as follows: Figure 3 shown. Figure 3 It shows that the metasurface is composed of an array of nanospheres with a diameter of about 100nm, which are generally arranged in a relatively uniform and dense state. In addition, the method was verified using mouse blood cells as the target analyte. After cleaning and sterilizing the metasurface-microfluidic chip and microsyringe, they were naturally dried. 4% of mouse red blood cells were drawn with a microsyringe and introduced into the prepared metasurface-microfluidic chip at a speed of 1μL / min. The chip was placed on a three-dimensional translation stage, and the lateral direction of the translation stage was adjusted so that the light spot was irradiated on the embedded metasurface. The vertical position of the translation stage was adjusted so that the light spot was focused on the metasurface-microfluidic interface. Figure 4 (a) The Raman spectrum of the microfluidic containing mouse red blood cells with a volume fraction of 4% was measured when the embedded metasurface-microfluidic chip was illuminated by the radially polarized vector light field and the linearly polarized light recorded by the spectrometer, and the integration time was 10 s. Figure 4 (a) It shows that the embedded metasurface-microfluidic chip has good biocompatibility. Compared with conventional linearly polarized light excitation, radially polarized vector light field excitation can significantly enhance the Raman spectral intensity of mouse red blood cell microfluidics by about 2 times, indicating that this scheme is reliable and effective in improving the detection sensitivity of biological cell Raman spectroscopy. At the same time, it has good biocompatibility and can realize label-free detection of biological cell microfluidics. Figure 4(bd) are the radially polarized vector light fields recorded by the spectrometer when irradiating the embedded metasurface-microfluidic chip, and the measured values ​​of different concentrations (10 -1 M, 10 -2 M, 10 -3 Raman spectra of mouse erythrocyte microfluidic in glucose solution (10 M) with an integration time of 10 s. -1 M, 10 -2 M, 10 -3 M) glucose aqueous solutions were mixed in equal volumes. Figure 4 (bd) After the glucose solution was added to the mouse red blood cell microfluid, the Raman peak was monitored to change, with the peak at 2201 cm -1 The Raman peak at appears with the addition of glucose, and tends to become stronger as the concentration of the glucose solution decreases, indicating that information about changes in the glucose concentration in the cellular environment can be extracted from this spectrum. This further shows that the configuration of embedded metasurface-microfluidic chip excited by radially polarized vector light field is expected to be used for blood glucose concentration monitoring and further expanded to the fields of pathological analysis, medical diagnosis, etc.

[0045] Through the above-mentioned embedded super surface-microfluidic chip and its preparation method, on the one hand, the metallized nano array is embedded in the microfluidic chip, and the array is fixed and uniform, which can not only eliminate the interference of the flow of metal nanoparticle dispersion in conventional SERS-microfluidic technology on the target detection object, realize label-free SERS-microfluidic technology, but also generate uniform hot spots to enhance the Raman signal of cells in the microfluid. On the other hand, the prepared embedded super surface-microfluidic chip has good biocompatibility and can significantly enhance the Raman spectrum intensity of biological cells. Using a cylindrical vector light field as the excitation light field, the vector light field can further enhance the Raman signal of the microfluidic compared to conventional linearly polarized light, thereby effectively reducing the light power required to excite the biological cell Raman, and avoiding damage to the biological cells to be tested caused by excessive excitation light power.

[0046] It should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", "clockwise", "counterclockwise", etc. in the above description indicate orientations or positional relationships based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present disclosure and simplifying the description, and do not indicate or imply that the referred device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the embodiments of the present disclosure.

[0047] In addition, the terms "first" and "second" are used for descriptive purposes only and should not be understood as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of the features. In the description of the embodiments of the present disclosure, the meaning of "multiple" is two or more, unless otherwise clearly and specifically defined.

[0048] In the embodiments of the present disclosure, unless otherwise clearly specified and limited, the terms "installed", "connected", "connected", "fixed" and the like should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present disclosure can be understood according to specific circumstances.

[0049] In the embodiments of the present disclosure, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include the first and second features being in direct contact, or may include the first and second features not being in direct contact but being in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes the first feature being directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0050] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "example", "specific example" or "some examples" etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present disclosure. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner. In addition, those skilled in the art may combine and combine the different embodiments or examples described in this specification.

[0051] Those skilled in the art will readily appreciate other embodiments of the present disclosure after considering the specification and practicing the invention disclosed herein. This application is intended to cover any modification, use or adaptation of the present disclosure, which follows the general principles of the present disclosure and includes common knowledge or customary techniques in the art that are not disclosed in the present disclosure. The specification and examples are intended to be exemplary only, and the true scope and spirit of the present disclosure are indicated by the appended claims.

Claims

1. A method for preparing an embedded supersurface-microfluidic chip, characterized in that: The method includes: Placing a first glass sheet in a container, injecting deionized water into the container until the first glass sheet is submerged, and injecting a PS ball layer onto the liquid surface of the deionized water in the container using a micro-injector at a preset volume rate; dripping a plurality of drops of sodium dodecyl sulfate aqueous solution at a plurality of positions along the side wall of the container to make the PS ball layer densely packed and arranged, and then releasing the deionized water in the container to make the PS ball layer drop along with the liquid level until the PS ball layer is deposited on the surface of the first glass sheet; Mixing equal volumes of silver ammonia solution and potassium sodium tartrate solution to form a mixed solution, spraying the mixed solution on the surface of the PS ball layer through a sprayer by a chemical spraying method, and standing until a silver single substance layer is generated on the surface of the PS ball layer to obtain a super surface array; Inverting the first glass sheet onto a second glass sheet carrying a microfluidic channel to embed the metasurface array into the microfluidic channel; wherein the first glass sheet is smaller than the second glass sheet; The first needle and the second needle are fixed to the edge of the first glass sheet and the second glass sheet using ultraviolet curing glue, and the ultraviolet curing glue is irradiated with ultraviolet light to cure it, so as to obtain an embedded metasurface-microfluidic chip; wherein the first needle is the inlet of the embedded metasurface-microfluidic chip, the second needle is the outlet of the embedded metasurface-microfluidic chip, and the first needle and the second needle are both connected to the microfluidic channel.

2. The method for preparing the embedded supersurface-microfluidic chip according to claim 1, characterized in that: The container is provided with a water control valve, through which the deionized water is injected into or released from the container, and the diameter of the water control valve is 2-5 mm.

3. The method for preparing the embedded supersurface-microfluidic chip according to claim 1, characterized in that: The diameter of the PS sphere layer is less than or equal to 300 nm, and the volume speed of the PS sphere layer injection is 10-50 μL / min.

4. The method for preparing the embedded supersurface-microfluidic chip according to claim 1, characterized in that: The mass fraction of the sodium dodecyl sulfate aqueous solution is 1%-5%, and the number of drops of the sodium dodecyl sulfate aqueous solution dropped is 1-3 drops.

5. The method for preparing the embedded supersurface-microfluidic chip according to claim 1, characterized in that: The concentration of the silver ammonia solution is 0.01 g / ml, and the concentration of the potassium sodium tartrate solution is 0.05 g / ml; wherein the silver ammonia solution is made of AgNO3 solution and ammonia water.

6. The method for preparing the embedded supersurface-microfluidic chip according to claim 1, characterized in that: In the chemical spraying method, the spraying direction of the sprayer is perpendicular to the plane of the PS ball layer, and the distance between the sprayer and the plane of the PS ball layer is greater than or equal to 10 cm.

7. The method for preparing the embedded supersurface-microfluidic chip according to claim 6, characterized in that: The spraying times of the sprayer is 2-4 times.

8. An embedded supersurface-microfluidic chip, characterized in that: Prepared by the preparation method according to any one of claims 1 to 7.

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