Artificial propagation method of pseudobagrus pseudobagrus

Abstract

The invention relates to the field of fish breeding, and discloses an artificial breeding method of pseudobagrus pseudobagrus, which comprises the following steps: firstly, feeding parent fishes of the pseudobagrus pseudobagrus with feed in a parent breeding pond; a water pump is used for producing flowing water to stimulate gonad development of the pseudobagrus pseudobagrus parent fish; after the sexual gland of the pseudobagrus pseudobagrus parent fish is stimulated to develop, the pseudobagrus pseudobagrus parent fish is caught, and oxytocic injection is conducted on the pseudobagrus pseudobagrus parent fish at the front stage and the back stage; the method comprises the following steps: placing eggs and seminal fluid in a water container, carrying out artificial insemination in a wet insemination mode to obtain fertilized eggs, placing the fertilized eggs in a flowing water pool, waiting for incubation of the fertilized eggs until fish fries come out of membranes, placing the fish fries in a cultivation pool of zooplanktons, fishing the fish fries when the fish fries grow to 3-6cm, and culturing the fish fries in an indoor glass jar to complete the artificial propagation process of the pseudobagrus pseudobagrus. The method solves the problems that domestic pseudobagrus concavata is in a short-supply state for a long time, and artificial propagation of pseudobagrus concavata is not broken through in China at present.

Description

Technical Field

[0001] The invention relates to the field of fish breeding, and in particular to an artificial breeding method for Pseudocaurus concavoura. Background Art

[0002] Pseudobagrus emarginatus belongs to the order Siluriformes, family Pseudobagrus, genus Pseudobagrus. Pseudobagrus emarginatus has a long, short body, a flat head, and a gradually flattened body after the head. The top of the head is covered with thin skin. The snout is flat and round. The mouth is sub-inferior, the mouth cleft is wide and slightly arc-shaped, and the upper and lower jaws and palatine bones have fine velvety teeth. The upper lip is thick, the upper and lower lips are connected at the corner of the mouth, and the posterior lip groove is discontinuous. There are 4 pairs of whiskers, the nasal whiskers can reach the middle point of the eyes, and the end of the upper jaw whiskers can reach the gill membrane. In a few individuals, they cannot reach the gill membrane. The eyes are small, laterally superior, and the nostrils are separated. The posterior nostril is closer to the eye than the anterior nostril. The gill opening is wide, and the gill membrane is united on the left and right but not connected to the isthmus. The dorsal fin has thin hard spines and no serrations on the rear edge. The pectoral fins have thick hard spines, the front edge is smooth, and the rear edge has developed serrations. The end of the pelvic fin extends beyond the anus but does not reach the starting point of the anal fin. The base of the anal fin is shorter than that of the adipose fin, the caudal fin is concave, and the shortest central fin is about 2 / 3 of the longest fin ray.

[0003] The concave-tailed catfish is a high-quality fish unique to the upper reaches of the Yangtze River. It mainly lives at the bottom of rivers or streams. It is carnivorous. It matures early, reaching sexual maturity at around 140mm. The eggs are golden yellow, with a diameter of about 2.2mm. The concave-tailed catfish has long been in short supply in China, and the country has not yet made a breakthrough in artificial breeding of this fish. Summary of the invention

[0004] The invention aims to provide an artificial propagation method of the concave tail catfish, so as to solve the problem that the concave tail catfish has been in short supply for a long time in China and the artificial propagation of the fish has not been broken through in China at present.

[0005] In order to achieve the above object, the present invention provides the following method:

[0006] The present invention provides an artificial propagation method of Pseudocaurus concavoura, which comprises:

[0007] S1: feeding the broodstock of Pseudocalis sutchuenensis with a feed having a protein content of ≥40% in a parent culture pond until the broodstock of Pseudocalis sutchuenensis reaches 4 years old;

[0008] S2: using a water pump to produce flowing water in the parent culture pond to simulate the scouring of river water, thereby stimulating the gonadal development of the parent fish of Pseudocalis concavotail;

[0009] S3: After stimulating the gonadal development of the Pseudocalis concavotail broodstock, the Pseudocalis concavotail broodstock are caught and subjected to two stages of oxytocin injection, namely, a first injection stage and a second injection stage;

[0010] S4: placing the eggs and semen of the broodstock of the Pseudocalis concavotail injected with the oxytocin in a water container, and performing artificial insemination by wet insemination to obtain fertilized eggs;

[0011] S5: After artificial insemination by wet insemination, the fertilized eggs are placed in a running water pool for 3-5 minutes to wait for the fertilized eggs to hatch until the fry emerge;

[0012] S6: After the fry have emerged from the membrane, the water in the water pool is changed once a day, and an air pump is used to increase oxygen until the vitality of the fry is enhanced;

[0013] S7: When the vitality of the fry is enhanced, the fry is placed in a cultivation pond where zooplankton is cultivated. When the fry grows to 3 cm-6 cm, it is caught and taken to an indoor glass tank for cultivation, thereby completing the artificial breeding process of the concave tail catfish.

[0014] Preferably, the water temperature of the parent culture pond is controlled to be 23° C.-30° C., and the water depth is 80 cm-150 cm.

[0015] Preferably, a water pump with a power of 600W or more is used in the parent breeding pond to produce flowing water, simulating the scouring of river water, so as to stimulate the gonadal development of the male and female broodstock of Pseudocalis concavotail.

[0016] Preferably, the gonadal development of the Pseudocalis broodstock is stimulated until the ovaries of the female Pseudocalis broodstock reach stage IV, are in the shape of long sacs, fill the entire abdominal cavity, and appear slightly red, thick blood vessels appear in the ovarian membrane, forming a reticular distribution, and the eggs are filled with yolk and are as plump as millet grains, until the gonadal development of the male Pseudocalis broodstock reaches stage III, is in the shape of branched branches, and is milky white, thereby completing the process of stimulating the gonadal development of the Pseudocalis broodstock.

[0017] Preferably, the first injection stage is: injecting the female broodstock of Pseudocalis concavourae with 15 μg of A2 and 2 mg of DOM per kilogram of female fish, and maintaining the water temperature at 25°C-27°C.

[0018] Preferably, the second injection stage is: 10h-12h after the first injection stage, both the female P. concavotail catfish broodstock and the male P. concavotail catfish broodstock are injected, with A2: 20 μg, DOM: 2 mg, HCG: 1000 IU per kilogram of the P. concavotail catfish broodstock.

[0019] Preferably, the step of placing the eggs and semen of the oxytocin-injected Pseudocalis broodstock in a water container and performing artificial insemination by wet insemination to obtain fertilized eggs comprises: catching the female Pseudocalis broodstock and squeezing out the eggs of the female Pseudocalis broodstock; dissecting the male Pseudocalis broodstock, taking out the testis of the male Pseudocalis broodstock, and wiping off the moisture of the testis with a soft paper towel; placing the testis in a mortar and crushing it, adding a small amount of physiological saline to mix it, to obtain a testis-physiological saline mixture; pouring the testis-physiological saline mixture into the eggs to carry out a fertilization process to obtain fertilized eggs.

[0020] Preferably, the step of pouring the testis-physiological saline mixture into the egg to carry out a fertilization process to obtain a fertilized egg comprises: pouring the testis-physiological saline mixture into the egg, adding water and stirring rapidly for 10 seconds to 30 seconds, leaving it to stand for 2 to 5 minutes after stirring with water, waiting for the fertilization process to obtain a fertilized egg.

[0021] Preferably, after artificial insemination by wet insemination, after standing for 3-5 minutes, the method further includes washing away blood clots and mucous membranes on the eggs, and placing the eggs on a polyethylene hatching mesh; placing the polyethylene hatching mesh in a flowing water pool, waiting for the fertilized eggs to hatch into fry and emerge from the membrane; and maintaining the dissolved oxygen in the water body>6.0mg / L during the hatching process of the fertilized eggs.

[0022] Preferably, when the fry grows to 3cm-6cm, the step of catching them and taking them to an indoor glass tank for cultivation includes: when the fry grows to 3cm-6cm, catching them and taking them to an indoor glass tank, feeding them with frozen worms for 3-5 days, and carrying out the first stage of cultivation; after the first stage of cultivation, adding 20% ​​of powder to the frozen worms and diluting water to feed the fry, feeding twice a day for 8-10 days, and carrying out the second stage of cultivation; after the second stage of cultivation, adding the powder to 50%, after 20 days, adding the powder to 80%, and after 30 days, feeding with 1.0 sinking material to complete the artificial breeding process of the concave tail catfish.

[0023] The beneficial effects of the present invention are as follows: the present invention uses a water pump to produce flowing water in a parent breeding pond to simulate the scouring of river water, thereby stimulating the gonad development of the concave tail catfish parent fish, so that the gonads of the concave tail catfish parent fish reach an optimal state, and then controls the different optimal production states of male and female concave tail catfish parent fish through two injections of oxytocin, and then performs artificial insemination by wet fertilization. Through intensive parent cultivation, artificial injection of oxytocin and artificial insemination, large-scale breeding is achieved, breaking through the bottleneck of artificial breeding of domestic concave tail catfish parent fish. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following is a brief introduction to the drawings required for the specific embodiments or the description of the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn according to the actual scale.

[0025] Figure 1 The present invention is a schematic diagram of a process for artificial propagation of Pseudocaurus concavoura provided by an embodiment of the present invention. DETAILED DESCRIPTION

[0026] In order to make the technical personnel in the technical field better understand the scheme of the present invention, the technical scheme in the embodiment of the present invention will be clearly and completely described below in combination with the drawings in the embodiment of the present invention. Obviously, the described embodiment is only a part of the embodiment of the present invention, not all the embodiments. Based on the embodiment of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The terms "first", "second", etc. in the specification and claims of the present invention and the above-mentioned drawings are used to distinguish different objects rather than to describe a specific order. In addition, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusions. For example, a process, method, device, product or end including a series of steps or units is not limited to the listed steps or units, but may optionally include steps or units that are not listed, or may optionally include other steps or units inherent to these processes, methods, products or ends.

[0028] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present invention. The appearance of the phrase in various places in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0029] The concave-tailed catfish is a high-quality fish unique to the upper reaches of the Yangtze River. It mainly lives at the bottom of rivers or streams. It is carnivorous. It matures early, reaching sexual maturity at around 140mm. The eggs are golden yellow, with a diameter of about 2.2mm. The concave-tailed catfish has long been in short supply in China, and the country has not yet made a breakthrough in artificial breeding of this fish.

[0030] The invention aims to provide an artificial propagation method of the concave tail catfish, so as to solve the problem that the concave tail catfish has been in short supply for a long time in China and the artificial propagation of the fish has not been broken through in China at present.

[0031] A specific embodiment of the present invention provides an artificial propagation method of Pseudocaurus concavoura, the method comprising: Figure 1 As shown, the following steps are included:

[0032] S1: Feed the broodstock of Pseudocalis sutchuenensis with a protein content of ≥40% in the broodstock breeding pond until the broodstock of Pseudocalis sutchuenensis reaches 4 years old.

[0033] In the embodiment of the present invention, the water temperature of the parent culture pond is controlled to be 23℃-30℃, and the water depth is 80cm-150cm; daily management should be carried out during feeding, and water changes, feeding conditions, floating heads, dead fish and other phenomena should be observed every day. If problems are found, the causes should be found out in time and corresponding measures should be taken to solve them. If the parent fish of the concave tail catfish are red all over, the gills are white, the liver is white, and there is partial ascites. If not treated in time, it can cause a large number of deaths in a short time, and enrofloxacin can be mixed with feed and fed for 5-7 days.

[0034] S2: Use a water pump to create flowing water in the parent breeding pond to simulate the scouring of river water and stimulate the gonadal development of the parent fish of the concave tail catfish.

[0035] In an embodiment of the present invention, a water pump with a power of 600 W or more is used in a parent breeding pond to produce flowing water, simulating the scouring of river water, so as to stimulate the gonadal development of male and female broodstock of Pseudosphagnum broodstock; the gonadal development of Pseudosphagnum broodstock is stimulated until the ovaries of the female broodstock of Pseudosphagnum broodstock reach stage IV, are long cystic, fill the entire abdominal cavity, and appear slightly red, thick blood vessels appear in the ovarian membrane, forming a reticular distribution, and the eggs are filled with yolk and are as plump as millet grains, until the gonadal development of the male broodstock of Pseudosphagnum broodstock reaches stage III, is branched in the shape of branches, and is milky white, thereby completing the process of stimulating the gonadal development of the broodstock of Pseudosphagnum broodstock.

[0036] S3: After stimulating the gonadal development of the Pseudosphagnum catfish broodstock, the Pseudosphagnum catfish broodstock are caught and subjected to two stages of oxytocin injection, namely the first injection stage and the second injection stage.

[0037] In the embodiment of the present invention, the first injection stage is: injecting female broodstock of Pseudosphagna broodstock, A2: 15μg, DOM: 2mg per kilogram of female fish, and maintaining the water temperature at 25℃-27℃; the second injection stage is: 10h-12h after the first injection stage, injecting female broodstock of Pseudosphagna broodstock and male broodstock of Pseudosphagna broodstock, A2: 20μg, DOM: 2mg, HCG1000IU per kilogram of Pseudosphagna broodstock; selecting broodstock for inducing spawning: when selecting female broodstock, lie the fish body membrane upward, slightly exposed to the water surface, if the abdomen is swollen and soft, especially the two sides of the lower abdomen are slightly drooping, the midline is slightly concave, the ovary outline is obvious, and the genital pore is slightly red and slightly protruding, the spawning can be induced; the method of selecting male fish is: gently squeeze the two sides of the lower abdomen of the fish with hands, and the genital pore has milky white thick semen flowing out. And it quickly disperses after entering the water, it can be used for inducing spawning.

[0038] S4: The eggs and semen of the broodstock of the concave tail catfish injected with oxytocin are placed in a water container, and artificial insemination is performed by wet insemination to obtain fertilized eggs.

[0039] In an embodiment of the present invention, female broodstock of the concave tail catfish are caught and the eggs of the female broodstock of the concave tail catfish are squeezed out; male broodstock of the concave tail catfish are dissected, the testis of the male broodstock of the concave tail catfish is taken out, and the moisture of the testis is wiped off with a soft paper towel; the testis is placed in a mortar for crushing, and a small amount of physiological saline is added to mix well to obtain a testis-physiological saline mixture; the testis-physiological saline mixture is poured into the eggs for fertilization process to obtain fertilized eggs; the testis-physiological saline mixture is poured into the eggs for fertilization process to obtain the fertilized eggs, and the steps of pouring the testis-physiological saline mixture into the eggs, adding water and stirring quickly for 10 seconds to 30 seconds, and standing for 2 to 5 minutes after adding water and stirring, waiting for the fertilization process to obtain the fertilized eggs.

[0040] S5: After artificial insemination using the wet insemination method, let it stand for 3-5 minutes, place the fertilized eggs in a flowing water pool, and wait for the fertilized eggs to hatch until the fry emerge.

[0041] In an embodiment of the present invention, after artificial insemination by wet insemination, after standing for 3-5 minutes, the process further includes: washing away blood clots and mucous membranes on the eggs, and placing the eggs on a polyethylene hatching mesh; placing the polyethylene hatching mesh in a flowing water pool, waiting for the fertilized eggs to hatch into fry and emerge from the membrane; and maintaining the dissolved oxygen in the water body >6.0 mg / L during the process of waiting for the fertilized eggs to hatch.

[0042] S6: After the fry have hatched, change the water in the running pool once a day and use an air pump to add oxygen until the fry become more active.

[0043] S7: When the vitality of the fry increases, the fry are placed in a breeding pond where zooplankton is cultivated. When the fry grow to 3cm-6cm, they are caught and brought to an indoor glass tank for cultivation, thus completing the artificial breeding process of the concave-tailed catfish.

[0044] In the embodiment of the present invention, when the fry grows to 3cm-6cm, they are caught and taken to an indoor glass tank to be fed with frozen worms for 3-5 days to carry out the first stage of cultivation; after the first stage of cultivation, 20% of powder is added to the frozen worms and diluted with water to feed the fry, twice a day for 8-10 days to carry out the second stage of cultivation; after the second stage of cultivation, the powder is added to 50%, and after 20 days, the powder is added to 80%, and after 30 days, 1.0 sinking material is used to feed, completing the artificial breeding process of the concave tail catfish; the cultivation process also includes a sterilization process, by putting oxidized tantalum and cypress branches into the cultivation pool, the hatching pool can be sterilized harmlessly to avoid drug damage to fertilized eggs, and the oxidized tantalum can destroy the cell membrane of bacteria in the water through the super oxidizing ability of the photocatalyst, causing the bacterial mass to flow to death, coagulating the protein of the virus, and inhibiting the activity of the virus.

[0045] The beneficial effects of the present invention are as follows: the present invention uses a water pump to produce flowing water in a parent breeding pond to simulate the scouring of river water, thereby stimulating the gonad development of the concave tail catfish parent fish, so that the gonads of the concave tail catfish parent fish reach an optimal state, and then controls the different optimal production states of male and female concave tail catfish parent fish through two injections of oxytocin, and then performs artificial insemination by wet fertilization. Through intensive parent cultivation, artificial injection of oxytocin and artificial insemination, large-scale breeding is achieved, breaking through the bottleneck of artificial breeding of domestic concave tail catfish parent fish.

[0046] The above is only an embodiment of the present invention, and the common knowledge such as the known specific technical schemes or characteristics in the scheme is not described in detail here; it should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the scheme of the present invention, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the present invention and the practicality of the patent. The protection scope required by this application shall be based on the content of its claims, and the specific implementation methods and other records in the specification can be used to interpret the content of the claims.

Claims

1. An artificial propagation method of Pseudocaurus concavoura, characterized in that: The method comprises: S1: feeding the broodstock of Pseudocalis sutchuenensis with a feed having a protein content of ≥40% in a parent culture pond until the broodstock of Pseudocalis sutchuenensis reaches 4 years old; S2: using a water pump to produce flowing water in the parent culture pond to simulate the scouring of river water, thereby stimulating the gonadal development of the parent fish of Pseudocalis concavotail; S3: After stimulating the gonadal development of the Pseudocalis concavotail broodstock, the Pseudocalis concavotail broodstock are caught and subjected to two stages of oxytocin injection, namely, a first injection stage and a second injection stage; S4: placing the eggs and semen of the broodstock of the Pseudocalis concavotail injected with the oxytocin in a water container, and performing artificial insemination by wet insemination to obtain fertilized eggs; S5: After artificial insemination by wet insemination, the fertilized eggs are placed in a running water pool for 3-5 minutes to wait for the fertilized eggs to hatch until the fry emerge; S6: After the fry have emerged from the membrane, the water in the water pool is changed once a day, and an air pump is used to increase oxygen until the vitality of the fry is enhanced; S7: When the vitality of the fry is enhanced, the fry is placed in a cultivation pond where zooplankton is cultivated. When the fry grows to 3 cm-6 cm, it is caught and taken to an indoor glass tank for cultivation, thereby completing the artificial breeding process of the concave tail catfish.

2. The artificial propagation method of a concave tailed catfish according to claim 1, characterized in that: The water temperature of the parent culture pond is controlled to be 23° C.-30° C., and the water depth is controlled to be 80 cm-150 cm.

3. The artificial propagation method of a concave tailed catfish according to claim 1, characterized in that: A water pump with a power of more than 600W is used in the parent culture pond to produce flowing water, simulating the scouring of river water, so as to stimulate the gonadal development of the male and female broodstock of the concave tail catfish.

4. The artificial propagation method of a concave tailed catfish according to claim 3, characterized in that: The gonadal development of the Pseudostemon broodstock is stimulated until the ovaries of the female Pseudostemon broodstock reach stage IV, are in the shape of long sacs, fill the entire abdominal cavity, and appear slightly red, thick blood vessels appear in the ovarian membrane, forming a reticular distribution, and the eggs are filled with yolk and are as plump as millet grains, until the gonadal development of the male Pseudostemon broodstock reaches stage III, is in the shape of branched branches, and is milky white, thereby completing the process of stimulating the gonadal development of the Pseudostemon broodstock.

5. The artificial propagation method of a Pseudocaurus concavoura according to claim 1, characterized in that: The first injection stage is: injecting the female broodstock of the concave tail catfish, injecting A2: 15 μg, DOM: 2 mg per kilogram of female fish, and maintaining the water temperature at 25°C-27°C.

6. The artificial propagation method of a Pseudocaurus concavoura according to claim 1, characterized in that: The second injection stage is: 10h-12h after the first injection stage, the female and male broodstock of Pseudostemon sphenoides are injected, with A2: 20μg, DOM: 2mg, HCG 1000IU injected per kilogram of the broodstock of Pseudostemon sphenoides.

7. The artificial propagation method of Pseudocaurus concavus according to claim 1, characterized in that: The step of placing the eggs and semen of the parent fish of the concave tail catfish injected with an oxytocin in a water container and performing artificial insemination by wet insemination to obtain fertilized eggs comprises: Catching the female broodstock of the Pseudocalis concavotail and squeezing out the eggs of the female broodstock of the Pseudocalis concavotail; dissecting the male parent fish of the Pseudocalis concavotail, taking out the testis of the male parent fish of the Pseudocalis concavotail, and wiping off moisture from the testis with a soft paper towel; The testis is placed in a mortar and crushed, and a small amount of physiological saline is added and mixed to obtain a testis-physiological saline mixture; The testis-physiological saline mixture is poured into the egg to carry out a fertilization process to obtain a fertilized egg.

8. The artificial propagation method of Pseudocaurus concavoura according to claim 7, characterized in that: The step of pouring the testis-physiological saline mixture into the egg to carry out fertilization to obtain a fertilized egg comprises: The testis-physiological saline mixture is poured into the egg, and water is added and quickly stirred for 10 seconds to 30 seconds. After adding water and stirring, it is allowed to stand for 2 to 5 minutes to wait for the fertilization process to obtain a fertilized egg.

9. The artificial propagation method of a Pseudocaurus concavoura according to claim 1, characterized in that: After artificial insemination by wet insemination, the method further comprises washing away blood clots and mucous membranes on the egg and placing the egg on a polyethylene hatching mesh; Placing the polyethylene hatching mesh in a flowing water pool, waiting for the fertilized eggs to hatch into fry and emerge from the membrane; While waiting for the fertilized eggs to hatch, the dissolved oxygen in the water is maintained at>6.0 mg / L.

10. The artificial propagation method of Pseudocaurus concavoura according to claim 1, characterized in that: The step of catching the fry when they grow to 3 cm to 6 cm and taking them to an indoor glass tank for cultivation comprises: When the fry grows to 3 cm-6 cm, they are caught and taken to an indoor glass tank where they are fed with frozen worms for 3-5 days for the first stage of cultivation; After the first stage of cultivation, 20% of the powder is added to the frozen worms and then diluted with water to feed the fry, twice a day for 8-10 days, and then the second stage of cultivation is carried out; After the second stage of cultivation, the powder is added to 50%, and after 20 days, the powder is added to 80%. After 30 days, 1.0 sinking material is used for feeding to complete the artificial propagation process of the concave tail catfish.

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