Red increasing agent for rhododendron flowers as well as preparation method and use method of red increasing agent

Abstract

The invention discloses a rhododendron flower reddening agent as well as a preparation method and a use method thereof. The rhododendron flower reddening agent comprises ammonium ferrous sulfate hexahydrate, saccharides, zinc chloride, zinc powder, citric acid and dipotassium phosphate. The components not only provide a carbon source, a nitrogen source, a phosphorus source and a potassium source required for the formation of the color of the rhododendron flowers, but also promote the synthesis of the rhododendron flower pigment through the synergism of Fe < 2 + > and Zn < 2 + > contained in the components, so that the rhododendron is endowed with more brilliant color, and the flowering phase of the rhododendron is longer. The invention also provides a preparation method and a use method of the rhododendron flower red-increasing agent, so as to realize the red-increasing effect on the rhododendron flower color. The red-increasing agent is wide in raw material source, low in cost and beneficial to popularization and application.

Description

Technical Field

[0001] The invention belongs to the preparation technology of fine chemicals in the chemical industry, and particularly relates to a rhododendron flower redness enhancing agent and a preparation and use method thereof. Background Art

[0002] Rhododendron is one of the top ten famous flowers in China. It has a very high ornamental value and is widely used in landscaping. Rhododendron plants in my country are widely distributed and have rich flower colors. In order to meet the diverse needs of the people, in recent years, researchers have obtained many new varieties of rhododendrons through gene editing technology, such as Anku Rhododendron and Four Seasons Rhododendron. They have the characteristics of continuously growing buds and blooming flowers in spring, autumn and winter, bringing new business opportunities to the rhododendron market. However, in the middle and lower reaches of the Yangtze River, there are large differences in soil composition, heavy rain in spring, insufficient light in mountainous areas, and large changes in the climate environment in spring, autumn and winter. The new varieties of Anku Rhododendron and Four Seasons Rhododendron that bloom in three seasons have unstable growth, large changes in anthocyanin concentration, dull flower colors, and short flower opening life, which affect their commercial prospects for large-scale promotion and planting. Therefore, it is necessary to explore the biosynthesis mechanism of anthocyanins in this type of rhododendron to obtain the key technology for the cultivation of rhododendron color. Increasing the synthesis of azalea cyanines through chemical induction, maintaining the stable concentration of azalea cyanines, and achieving the synthesis regulation of azalea cyanines is a very valuable application work, and it is also expected to lay the foundation for solving the problem of dull color of azalea flowers.

[0003] Existing patents are limited to the color enhancement of plants to their fruits and leaves. For example, Patent No. 202210373811.0 and Patent No. 202210375551.0 are red enhancers that promote the coloring of citrus; Patent No. 201010596941.8 and Patent No. 201410766875.2 are color enhancers that promote the coloring of various fruits such as apples, grapes, cherries, dates, tomatoes and peppers; Patent No. 201910142738.4 is a color enhancer that makes red maple sprout, grow and fall leaves; Patent No. 201711090397.8 is a color enhancer that makes red maple leaves redder.

[0004] There are very few existing patents related to color enhancement of plant flowers. For example, Patent 201710689221.8 is related to a flower color enhancer, but the patent only involves the effect of extending the color retention period of flowers, and does not involve the color enhancement effect during the growth of flowers, nor does it involve how to regulate the synthesis of plant anthocyanins.

[0005] Since the composition of chromatin glycosides in each flower is different and their biosynthesis pathways may also be different, it is difficult to have a universal color enhancer to make the flowers of various plants more colorful. Azalea is a highly ornamental flower, and its flower pigments mainly include anthocyanins and flavonols. If the color effect of azalea is improved through chemical induction technology, and then the quality of azalea is improved, it will undoubtedly have practical value. However, there are very few technical reports related to this.

[0006] Potted azaleas are very ornamental and have great value in beautifying the indoor environment. As potted azaleas placed indoors, their advantage is that the soil, climate, temperature and humidity and other environmental factors of the growing village are controllable, but there are also problems such as insufficient light affecting the synthesis of chlorogenic acid glycosides and leaf growth of azaleas through photosynthesis, as well as high requirements for the hygiene and environmental protection of color regulators. Therefore, it is undoubtedly of great commercial value to invent a regulator that can not only regulate the color of azalea flowers, but also provide the nutrients needed to promote their growth, and has the characteristics of hygiene and environmental protection, thereby increasing the flowering life of azaleas.

[0007] In order to solve the above technical problems, the present invention provides a composition of a rhododendron flower reddening agent and its preparation and use method, which achieves the effect of reddening the color of rhododendron flowers and prolonging the blooming period of rhododendron flowers through the synergistic biocatalytic effect and antioxidant effect between chemical reagents. Summary of the invention

[0008] The invention provides a rhododendron flower redness enhancing agent, which aims to obtain potted rhododendrons with more gorgeous red flowers and white flowers that are redder, and to prolong their flowering period, so as to improve the ornamental value and commercial value of the potted rhododendrons.

[0009] The present invention is achieved through the following technical solutions:

[0010] A reddening agent for azalea flowers, characterized in that it comprises a buffer solution, wherein the buffer solution contains dissolved sugars, ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O] and zinc chloride, and dispersed with zinc powder.

[0011] As a further improvement, the sugar is a water-soluble sugar, and the water-soluble sugar includes glucose, sucrose, fructose, honey and sucralose.

[0012] As a further improvement, the zinc powder is ultrafine zinc powder with a mesh size of 800 or more.

[0013] Further improvement, the ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2·6H2O]: sugar: zinc chloride: zinc powder mass ratio is: 1.0: 7.0-10.0: 0.02-0.05: 0.005-0.010, wherein the final concentration of ammonium ferrous sulfate hexahydrate is 0.20%-0.25%.

[0014] As a further improvement, the preparation method of the buffer solution is as follows:

[0015] Weigh 10.8g to 13.6g of citric acid [C4H2O7·H2O] and dissolve it in 2000mL of water to prepare a citric acid solution;

[0016] Weigh 24.0g to 29.8g of dipotassium hydrogen phosphate (K2HPO4·3H2O) and dissolve it in 2000mL of water to prepare a dipotassium hydrogen phosphate solution;

[0017] The citric acid solution is mixed with the dipotassium hydrogen phosphate solution to obtain the buffer solution, and the pH value of the buffer solution is between 4.5 and 5.0.

[0018] A method for preparing a rhododendron flower redness enhancing agent comprises the following steps: firstly preparing the buffer solution as described above, and then dissolving ammonium ferrous sulfate hexahydrate: sugar: zinc chloride in a mass ratio of 1.0: 7.0-10.0: 0.02-0.05 into the buffer solution, wherein the final concentration of the ammonium ferrous sulfate hexahydrate is 0.20%-0.25%; within 2 hours before the redness enhancing agent is used, zinc powder is added in a mass ratio of ammonium ferrous sulfate hexahydrate: zinc powder in a mass ratio of 1: 0.005-0.010, and the mixture is fully stirred.

[0019] A method for using a rhododendron flower redness enhancing agent, the rhododendron flower redness enhancing agent is as described above, and the method for using is as follows: when the first flower bud of the rhododendron flower just begins to expose petals, the rhododendron flower redness enhancing agent is sprayed within 30 cm around the root of the rhododendron flower in the bud stage within 2 hours after preparation, and sprayed once every 3 to 5 days, for a total of 3 to 5 times, with an addition amount of 20 to 60 mL each time.

[0020] The azalea flower redness enhancing agent of the present invention is suitable for azaleas with purple-red flowers, mixed-color flowers of white and red, and white flowers, and has no obvious brightening effect on darker bright red flowers.

[0021] The experimental object of the azalea flower redening agent of the present invention is the potted four-season azalea cultivated by the enterprise. This variety can bloom in three seasons, so it is conducive to seeing the color enhancement effect of the azalea flowers. According to the cultivation of azalea by the redening agent and the color change mechanism of its flowers, other varieties of azalea can be inferred.

[0022] The sugar in the red-enhancing agent for azalea flowers of the present invention provides the carbon source required for the glycosylation of azalea plant growth. It is a water-soluble sugar, and glucose, which is cheap, is preferably used. The mass ratio of ammonium ferrous sulfate to sugar is preferably 1:7.0-10.0. In addition to providing ferrous ions, the ammonium ferrous sulfate in the red-enhancing agent for azalea flowers of the present invention also provides the nitrogen source [(NH4)2·SO4] required for the growth of azaleas. In addition, the dipotassium hydrogen phosphate in the red-enhancing agent for azalea flowers of the present invention provides the required phosphorus source and potassium source at the same time, which can avoid the adverse effects of excessive inorganic ion concentration on the growth of azaleas. In short, the red-enhancing agent for azalea flowers of the present invention covers the elements such as C, N, P, and K required for the growth of azaleas, and meets the cleaning and environmental protection requirements required by ornamental potted azaleas.

[0023] The ammonium ferrous sulfate [Fe(NH4)2·(SO4) 2· 6H2O] plays an important role in the synthesis of anthocyanins in azalea. Our research has shown that the red color of azalea flowers is related to the content of anthocyanins in azalea flowers, that is, the higher the content of anthocyanins, the more red the azalea flowers are. We found that Fe 2+ The five-membered ring chelate formed with the o-diphenol hydroxyl derivatives in the anthocyanins of rhododendron promotes the synthesis of anthocyanins of rhododendron, playing an important role in increasing the biosynthesis of anthocyanins of rhododendron. At the same time, the five-membered ring chelate formed has a conjugated large ∏ bond, which red-shifts the maximum visible absorption wavelength, that is, the Fe 2+ The reddening effect ( Figure 1a ).

[0024] The redness enhancing agent for azalea flowers of the present invention uses ammonium ferrous sulfate [Fe(NH4)2·(SO4) 2· 6H2O], rather than ammonium ferric sulfate or ferrous sulfate. The principle is that, from the perspective of ionic radius, Fe 3+ The radius (0.056nm) is much smaller than Fe 2+ The radius of Fe is 0.078nm, so it cannot form a stable five-membered ring chelate, that is, it cannot form a conjugated large ∏ bond, and thus will not produce a reddening effect. 2+ It is unstable in slightly acidic solution and is easily oxidized to Fe 3+, When the pH value is greater than 3.2, the oxidized Fe 3+ Completely precipitated as Fe(OH)3, Fe 3+ Lost the opportunity to participate in the synthesis of safflower cyanin; when pH = 4.5 ~ 7.0, Fe 2+ No hydrolysis occurs to form Fe(OH)2 precipitate, i.e. Fe 2+It can participate in the synthesis of azalea anthocyanin and form a stable five-membered ring chelate with it, causing the maximum visible absorption wavelength to red shift, that is, causing the azalea to appear Fe 2+ The reddening effect.

[0025] The first important problem solved by the present invention is to avoid or reduce Fe 2+ Oxidized to Fe 3+ , to ensure a stable amount of Fe 2+ Participate in the biochemical synthesis of azalea anthocyanins. Its means are:

[0026] First, the redness enhancing agent for azalea flowers of the present invention does not use a combination of ferrous sulfate and ammonium sulfate, but uses ammonium ferrous sulfate hexahydrate with crystal water. This ammonium ferrous sulfate with crystal water can effectively avoid Fe 2+ Oxidized to Fe 3+ , ensuring sufficient Fe 2+ The concentration participates in the synthesis of azalea anthocyanin and forms a stable five-membered ferrous ion chelate. The conjugated large ∏ bond formed causes its maximum visible absorption wavelength to red shift, so Fe appears in the azalea flower color induction experiment. 2+ In order to ensure that there are enough ferrous ions to participate in the synthesis of azalea anthocyanins, ammonium ferric sulfate or single ferrous sulfate is not used.

[0027] Secondly, zinc chloride is used in the redness enhancing agent for azalea flowers of the present invention, and zinc chloride can provide Zn 2+ , Zn 2+ The radius (0.074nm) and Fe 2+ The radius (0.078nm) is close, and it can also form a stable zinc ion five-membered chelate, Zn 2+ with Fe 2 + The synthesis of anthocyanin in rhododendron was promoted synergistically, thereby increasing the biosynthesis of anthocyanin in rhododendron. 2+ The ions will not be oxidized and can exist stably in a buffer solution with a pH value of 4.5 to 5.0, which makes up for the Fe 2+ ions are oxidized to Fe 3+ Make Fe 2+ The reduction in concentration leads to a decrease in the amount of anthocyanin, which causes the flower color to become lighter. 2+ 、Zn 2+ The concentration of metal ions can easily cause dehydration in plant cells, resulting in the withering of leaves and buds of azalea seedlings. Therefore, the mass ratio of ammonium ferrous sulfate hexahydrate to zinc chloride is controlled to be 1.0:0.02-0.05, that is, Fe 2+ / Zn 2+The molar ratio is about 1.0:0.05-0.14, and the final concentration of ammonium ferrous sulfate hexahydrate needs to be controlled to be 0.20%-0.25%.

[0028] Again, the azalea flower reddening agent of the present invention uses ultrafine zinc powder of 800 mesh or more, and the ultrafine zinc powder used can be relatively evenly distributed in a buffer solution with a pH value of 4.5 to 5.0, and can avoid Fe 2+ Oxidized to Fe 3+ In this way, during a long period of alternating spraying of reddening agent and tap water, the Fe required for biochemical synthesis of azalea anthocyanins was ensured. 2+ The concentration of Zn 2+ The concentration can achieve the effect of stable reddening of azalea flowers at different flowering periods. Due to the compensatory auxiliary effect of zinc powder, the amount of zinc powder used does not need to be too high, with the mass ratio of ammonium ferrous sulfate hexahydrate to zinc powder = 1: 0.005-0.010, and the molar ratio is about 1.0: 0.03-0.06.

[0029] The second important problem solved by the present invention is to achieve the effect of increasing the redness of the azalea flowers while extending the life of the azalea flowers.

[0030] Under light, Fe 2+ Easily oxidized to Fe 3+ , and then precipitated as Fe(OH)3, destroying the stability of the ferrous ion chelate, causing the color of the azalea flowers to fade easily, and then accelerating withering. The zinc ions in the zinc chelate will not be oxidized under light, nor will they form Zn(OH)2 precipitation in a buffer solution with a pH value of 4.5-5.0. The zinc ion five-membered chelate formed by it is more stable than the five-membered chelate ring of ferrous ions, thereby extending the flowering period of the azalea flowers (see Table 1 and Table 2). Since the content of anthocyanin in azalea is not very high, a small amount of zinc ions can enter the petal cells to form a stable zinc ion five-membered chelate, so the zinc ion concentration does not need to be too high.

[0031] The beneficial effects of the present invention are to achieve reddening of azalea flowers and to prolong the flowering period of the azalea flowers, and the reddening effect is particularly obvious for light-colored flowers. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1a Fe 2+ Five-membered ring chelate formed with chromatin;

[0033] Figure 1b Zn 2+ Five-membered ring chelate formed with chromatin;

[0034] Figure 2aThe color diagram of the purple azalea flower cultivated in Example 1;

[0035] Figure 2b The color diagram of the purple azalea flower cultivated in Comparative Example 5;

[0036] Figure 3a This is a picture of the azalea cultivated in Example 2;

[0037] Figure 3b This is the flower color of the azalea cultivated in Example 9 (partial). DETAILED DESCRIPTION

[0038] The technical solutions in the embodiments of the present invention will be clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0039] Example 1

[0040] 1. Preparation of citric acid and potassium phosphate buffer solution

[0041] Weigh 13.6 g of citric acid (C4H2O7·H2O) and dissolve it in 2000 mL of water to prepare a 0.68% citric acid solution;

[0042] Weigh 29.8 g of dipotassium hydrogen phosphate (K2HPO4·3H2O) and dissolve it in 2000 mL of water to prepare a 1.45% dipotassium hydrogen phosphate salt solution.

[0043] The above 970 mL of citric acid solution was mixed with 1030 mL of dipotassium hydrogen phosphate solution to obtain about 2000 mL of citric acid and dipotassium hydrogen phosphate buffer solution with a pH value of 4.8.

[0044] Note: For substances with crystalline water, the molecular weight of crystalline water is included in the mass percentage concentration, the same below.

[0045] 2. Preparation of azalea flower reddening agent solution

[0046] Weigh 5.01 g of ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O], 0.102g zinc chloride and 50.0g glucose are dissolved in the aforementioned 2000mL citric acid and dipotassium hydrogen phosphate buffer solution, stirred thoroughly to dissolve evenly, and then 0.050g zinc powder is added and stirred evenly to obtain a rhododendron flower reddening agent solution containing ammonium ferrous sulfate hexahydrate with a concentration of about 0.24%.

[0047] 3. Application of azalea flower reddening agent solution

[0048] Select the four-season azalea with purple-red flowers as the experimental object. On the first day when the flower buds of the four-season azalea with purple-red flowers show a little red, spray the newly prepared azalea flower reddening agent solution (ready-to-use) within 30 cm around the roots of the azalea. Spray once every 3 days, 60 mL each time, for a total of 4 times.

[0049] 4. Recording of the life span of azalea flowers and determination of the chromaticity value of azalea petals

[0050] From the time when the azalea buds just exposed the purple-red petals to the time when the petals fell off, the number of days each flower was open was recorded, and then the average number of days of opening was taken as the flower life span. The experimental results are shown in Table 1. Figure 2a .

[0051] The 3nh NR110 handheld colorimeter was used to measure two flowers of the same azalea plant that opened at the same time. The chromaticity was measured at the close positions of the five petals of each flower, and the average red chromaticity (a) was calculated. The results are shown in Table 1.

[0052] Comparative Example 1

[0053] According to the preparation process of the azalea flower redness enhancer solution in Example 1, ferrous sulfate was used instead of ammonium ferrous sulfate (the ferrous sulfate contained was of the same mass), and the purple-red flower four-season azalea seedlings cultivated in the same batch were subjected to a comparative experiment according to process 3 in the aforementioned Example 1, and recorded and measured according to the aforementioned process 4. The experimental results are shown in Table 1.

[0054] Comparative Example 2

[0055] According to the preparation process of the azalea flower redness enhancer solution in Example 1, but without adding zinc powder, a comparative experiment was conducted on the purple-red flower four-season azalea seedlings cultivated in the same batch according to process 3 in the aforementioned Example 1, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 1.

[0056] Comparative Example 3

[0057] According to the preparation process of the azalea flower redness enhancer solution in Example 1, but without adding zinc chloride and zinc powder, a comparative experiment was conducted on the purple-red flower four-season azalea seedlings cultivated in the same batch according to process 3 in the aforementioned Example 1, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 1.

[0058] Comparative Example 4

[0059] According to the preparation process of the azalea flower redness enhancer solution in Example 1, but without adding glucose, the purple-red flower four-season azalea seedlings cultivated in the same batch were subjected to a comparative experiment according to process 3 in the aforementioned Example 1, and recorded and measured according to the aforementioned process 4. The experimental results are shown in Table 1.

[0060] Comparative Example 5

[0061] Tap water was directly used to replace the azalea flower redness enhancer solution in Example 1, and a comparative experiment was conducted on the purple-red flower four-season azalea seedlings cultivated from the same batch according to process 3 in the aforementioned Example 1, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 1, Figure 2b .

[0062] Table 1: Comparison of the results of the experiment on the cultivation of purple-red flowers of Rhododendron sempervirens by red-enhancing agent solution and other solutions

[0063]

[0064] Comparing Example 1 with Comparative Examples 1 to Comparative Examples 5, the results show that: 1) the red color of the four-season azalea flower cultivated with the red-enhancing agent solution is significantly redder than that of the other five comparative examples, and its red chromaticity value is respectively about 10%, 17%, 24%, 18%, and 33% higher on average; 2) the life span of the four-season azalea flower cultivated with the red-enhancing agent solution is also significantly longer, and its life span is respectively about 7%, 18%, 20%, 21%, and 35% higher on average. Moreover, the life span of the four-season azalea flower cultivated with the red-enhancing agent solution is about 18% higher on average than that without zinc powder.

[0065] In short, the azalea reddening agent can make the flowers of the purple-flowered four-season azalea redder and prolong the flowering period. Ammonium ferrous sulfate, glucose, zinc chloride and zinc powder play a synergistic role in achieving the reddening of azalea.

[0066] Example 2

[0067] 1. Preparation of citric acid and potassium phosphate buffer solution

[0068] Weigh 10.8 g of citric acid (C4H2O7·H2O) and dissolve it in 2000 mL of water to prepare a 0.54% citric acid solution;

[0069] Weigh 24.0 g of dipotassium hydrogen phosphate (K2HPO4·3H2O) and dissolve it in 2000 mL of water to prepare a 1.2% dipotassium hydrogen phosphate solution;

[0070] The above 1020 mL of citric acid solution was mixed with 980 mL of potassium phosphate solution to obtain about 2000 mL of citric acid and dipotassium hydrogen phosphate buffer solution with a pH value of about 4.6.

[0071] 2. Preparation of azalea reddening agent solution

[0072] Weigh 4.21 g of ammonium ferrous sulfate [Fe(NH4)2·(SO4) 2·6H2O], 0.208g zinc chloride, 25.0g glucose and 5g sucrose are dissolved in about 2000mL of the aforementioned citric acid and potassium dihydrogen phosphate buffer solution, stirred thoroughly to dissolve evenly, and then 0.022g zinc powder is added and stirred evenly to obtain a rhododendron flower reddening agent solution with an ammonium ferrous sulfate concentration of about 0.20%.

[0073] 3. Application of azalea flower reddening agent solution

[0074] Select white-flowered azalea as the experimental object. On the first day when the buds of the white-flowered azalea show a little white, spray the azalea reddening agent solution within 30 cm from the roots of the azalea. Spray once every 4 days, 50 mL each time, for a total of 3 times.

[0075] 4. Recording of the life span of azalea flowers and determination of the chromaticity value of azalea petals

[0076] From the time when the white petals of the azalea buds just appeared until the petals fell off, the number of days each flower was open was recorded, and then the average number of days of opening was taken as the flower life span. The results are shown in Table 2. Figure 3a .

[0077] The 3nh NR110 handheld colorimeter was used to measure two flowers of the same azalea plant that opened at the same time. The chromaticity was measured at the close positions of the five petals of each flower, and the average red chromaticity (a) was calculated. The results are shown in Table 2.

[0078] Comparative Example 6

[0079] According to the preparation process of the azalea flower redness enhancer solution in Example 2, ferrous sulfate was used instead of ammonium ferrous sulfate (the ferrous sulfate contained was of the same mass), and a comparative experiment was conducted on the white flower four-season azalea seedlings cultivated in the same batch according to process 3 in the aforementioned Example 2, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 2.

[0080] Comparative Example 7

[0081] According to the preparation process of the azalea flower redness enhancer solution in Example 2, but without adding zinc powder, a comparative experiment was conducted on the white flower four-season azalea seedlings cultivated in the same batch according to process 3 in the aforementioned Example 2, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 2.

[0082] Comparative Example 8

[0083] A citric acid and dipotassium hydrogen phosphate buffer solution with a pH value of 5.5 was adjusted using 0.54% citric acid solution and 1.2% dipotassium hydrogen phosphate solution. Then, according to the preparation process of the azalea flower redness enhancer solution in Example 2, a comparative experiment was conducted on the white flower four-season azalea seedlings cultivated in the same batch according to process 3 in the aforementioned Example 2, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 2.

[0084] Comparative Example 9

[0085] Tap water was directly used to replace the azalea flower redness enhancer solution in Example 2, and a comparative experiment was conducted on the white flower four-season azalea seedlings cultivated from the same batch according to process 3 in the aforementioned Example 2, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 2, Figure 3b .

[0086] Comparative Example 10

[0087] According to the preparation process of the azalea flower redness enhancer solution in Example 2, honey was used instead of glucose, and a comparative experiment was conducted on the white flower four-season azalea seedlings cultivated in the same batch according to process 3 in the aforementioned Example 2, and records and measurements were performed according to the aforementioned process 4. The experimental results are shown in Table 2.

[0088] Comparing the above-mentioned Example 2 with Comparative Examples 6 to 10, the results show that: 1) The white petals of the white flower Rhododendron sibiricum cultivated with the red-enhancing agent solution are significantly redder than those of Comparative Examples 6 to 9, and their red chromaticity values ​​are respectively about 20%, 60%, 113%, and 260% higher on average. 2) The flowering period of the white flower Rhododendron sibiricum cultivated with the red-enhancing agent solution is longer than that of Comparative Examples 6 to 9, and the patented component has a 37% longer flowering period than the flower cultivated with tap water. 3) The increase in the red chromaticity value of the white flower Rhododendron sibiricum cultivated with the red-enhancing agent solution is significantly higher than the percentage increase in the red chromaticity value of the purple-red flower Rhododendron sibiricum, that is, the red-enhancing efficiency of light-colored flowers is higher. 4) The white flower Rhododendron sibiricum cultivated with the red-enhancing agent solution obtained by replacing glucose with honey has the same effect of reddening and extending the flowering period.

[0089] In short, the red-enhancing agent for azalea can change pure white azalea flowers into light red and white mixed-color flowers, and the life span of the flowers is prolonged. The pH value control of ammonium ferrous sulfate, honey, zinc chloride, zinc powder and buffer solution plays a synergistic role in achieving the red-enhancing effect and extending the flowering period of azalea flowers.

[0090] The experimental results of Example 2 and Comparative Example 9 on potted azalea are shown in the following figure. Figure 3a , Figure 3b .

[0091] Table 2: Comparison of the results of the cultivation experiment of the white flower Rhododendron sempervirens solution and other solutions

[0092]

[0093] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not describe all details in detail, nor do they limit the invention to only the specific implementation methods described.

Claims

1. A reddening agent for azalea flowers, characterized in that: The invention comprises a buffer solution, wherein sugar, ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O] and zinc chloride, and dispersed with zinc powder.

2. The redness enhancing agent for azalea flowers as claimed in claim 1, characterized in that The sugar is a water-soluble sugar, and the water-soluble sugar includes glucose, sucrose, fructose, honey and sucralose.

3. The redness enhancing agent for azalea flowers as claimed in claim 1, characterized in that The zinc powder is ultrafine zinc powder with a mesh size of 800 or more.

4. The redness-enhancing agent for azalea flowers as claimed in claim 1, characterized in that The ammonium ferrous sulfate hexahydrate [Fe(NH4)2·(SO4) 2· 6H2O]: sugar: zinc chloride: zinc powder mass ratio is 1.0: 7.0-10.0: 0.02-0.05: 0.005-0.010, wherein the final concentration of ammonium ferrous sulfate hexahydrate is 0.20%-0.25%.

5. The redness enhancing agent for azalea flowers as claimed in claim 4, characterized in that The preparation method of the buffer solution is as follows: weigh 10.8 g to 13.6 g of citric acid (C4H2O7·H2O) and dissolve it in 2000 mL of water to prepare a citric acid solution; Weigh 24.0g to 29.8g of dipotassium hydrogen phosphate (K2HPO4·3H2O) and dissolve it in 2000mL of water to prepare a dipotassium hydrogen phosphate solution; The citric acid solution and the dipotassium hydrogen phosphate solution are mixed to obtain the buffer solution, and the pH value of the buffer solution is 4.5-5.

0.

6. A method for preparing a rhododendron flower redness enhancing agent, characterized in that: The steps are as follows: first prepare the buffer solution according to claim 5, and then dissolve ammonium ferrous sulfate hexahydrate: sugar: zinc chloride in a mass ratio of 1.0: 7.0-10.0: 0.02-0.05 into the buffer solution, wherein the final concentration of ammonium ferrous sulfate hexahydrate is 0.20%-0.25%; within 2 hours before the use of the reddening agent, add zinc powder in a mass ratio of ammonium ferrous sulfate hexahydrate: zinc powder = 1: 0.005-0.010, and stir thoroughly.

7. A method for using a rhododendron flower redness enhancing agent, characterized in that: The azalea flower reddening agent is as described in any one of claims 1 to 6, and the method of use is as follows: when the first azalea bud just begins to reveal petals, spray the azalea flower reddening agent within 30 cm around the roots of the azalea in the bud stage within 2 hours after preparation, spray once every 3 to 5 days, for a total of 3 to 5 times, and add 20 to 60 mL each time.

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