Bio-organic fertilizer for preventing and treating root rot of polygonatum sibiricum as well as preparation method and application of bio-organic fertilizer

Abstract

The invention relates to the technical field of fertilizer preparation, and particularly discloses a biological organic fertilizer for preventing and treating rhizoma polygonati root rot as well as a preparation method and application thereof, the biological organic fertilizer is prepared from the following components in parts by weight: 1-3 parts of potassium fulvate, 2-4 parts of cortex phellodendri sawdust, 1-3 parts of citrus pulp, 1-3 parts of tobacco slag and 0.5-1.5 parts of a microbial agent; the microbial agent is composed of an agrobacterium radiobacter solution and a paenibacillus polymyxa solution according to a volume ratio of (4-8): (1-2). The biological organic fertilizer prepared by the invention not only can prevent and treat root rot of polygonatum cyrtonema, but also can promote the growth and development of polygonatum cyrtonema.

Description

Technical Field

[0001] The invention relates to the technical field of fertilizer preparation, and in particular to a biological organic fertilizer for preventing and treating polygonatum root rot, and a preparation method and application thereof. Background Art

[0002] Polygonatum cyrtonema Hua is a perennial herbaceous plant of the Asparagaceae family and the genus Polygonatum. Its stem is root-like; its nodes are swollen, one end of the internode is thick and the other end is thin, and the thick end has short branches; the leaves are arranged in whorls of 4 to 6, linear-lanceolate, with curled or curved tips; the inflorescence often has 2 to 4 flowers, umbel-shaped, with long, drooping peduncles; the bracts grow at the base of the pedicels, are membranous, awl-shaped or linear-lanceolate, the perianth is milky white or light yellowish yellow, and the middle part of the tube is slightly constricted; the berries are black when ripe. The flowering period is May to June, and the fruiting period is August to September. It is recorded in Baopuzi: "In the past, people believed that this product obtained the energy of Kun soil and the essence of heaven and earth, hence the name."

[0003] Polygonatum sibiricum grows in the edge of mountain forests, in shrubs or in semi-shaded areas on hillsides, and is distributed in various areas north of the Yangtze River. Polygonatum sibiricum has strong selectivity for its growth environment, preferring to grow in a humid environment, with loose and fertile soil, sufficient surface moisture, shade, and sufficient light transmittance in the edge of the forest; it is suitable for soils with moderate pH and slightly acidic; it can be propagated by rhizomes and seeds, with rhizomes being the main propagation.

[0004] The diseases of Polygonatum sibiricum mainly include Polygonatum sibiricum black spot disease, Polygonatum sibiricum leaf spot disease, Polygonatum sibiricum anthracnose disease and root rot disease. Among them, Polygonatum sibiricum root rot disease mainly infects the roots, and the initial symptoms are not obvious, but as the disease progresses, irregular dark brown spots will appear on the tubers, eventually leading to tuber rot. Prevention and control strategies include strengthening field management, keeping the soil loose and at a suitable humidity, and avoiding excessive fertilization. In the early stages of the disease, an appropriate amount of fungicide can be used for root irrigation treatment. However, fungicides have a negative impact on plant growth and soil environment, and are not easy to use for a long time. Existing fertilizers are mainly used to provide fertility, and it is difficult to take into account the prevention and control of Polygonatum sibiricum root rot disease, and most products can only prevent and control a single pathogen. Therefore, it is urgent to develop a fertilizer that can effectively prevent and control Polygonatum sibiricum root rot disease. Summary of the invention

[0005] In order to develop a fertilizer capable of preventing and treating root rot of polygonatum, the present invention provides a biological organic fertilizer for preventing and treating root rot of polygonatum, a preparation method and application thereof. The biological organic fertilizer prepared by the present invention can not only prevent and treat root rot of polygonatum, but also promote the growth and development of polygonatum multiflorum.

[0006] The invention provides a biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema, the biological organic fertilizer is composed of the following components in parts by weight: 1 to 3 parts of potassium humate, 2 to 4 parts of yellow cedar sawdust, 1 to 3 parts of citrus residue, 1 to 3 parts of tobacco residue, and 0.5 to 1.5 parts of microbial agent;

[0007] The microbial agent is composed of Agrobacterium radiobacter bacterial solution and Paenibacillus polymyxa bacterial solution in a volume ratio of 4-8:1-2.

[0008] The biological organic fertilizer prepared by the present invention can not only prevent and treat root rot of polygonatum cyrtonema caused by pathogenic bacteria Fusarium oxysporum and Fusarium solani, but also promote the growth and development of polygonatum cyrtonema; wherein the microbial agent can significantly improve the prevention and treatment effect of organic fertilizer on root rot of polygonatum cyrtonema. Moreover, it can promote the growth and development of polygonatum cyrtonema, and the radiobacterium and the polymyxa bacillus act synergistically.

[0009] Furthermore, the microbial agent is composed of a bacterial solution of Agrobacterium radiobacterium and a bacterial solution of Paenibacillus polymyxa in a volume ratio of 6:1.

[0010] Furthermore, the biological organic fertilizer is composed of the following components in parts by weight: 1 part of potassium humate, 4 parts of yellow cedar sawdust, 2 parts of citrus residues, 3 parts of tobacco residues, and 1 part of microbial agent.

[0011] Furthermore, the biological organic fertilizer is composed of the following components in parts by weight: 2 parts of potassium humate, 3 parts of yellow cedar sawdust, 2 parts of citrus residues, 2 parts of tobacco residues, and 1.5 parts of microbial agents.

[0012] Furthermore, the preparation process of the radial Agrobacterium bacterial solution is as follows: first, the radial Agrobacterium is streaked and inoculated to activate, and after transfer 3 to 4 times, a single colony is picked and inoculated into a liquid culture medium, and cultured at 35°C to 37°C and 150rpm to 180rpm until the OD600 is 0.7 to 1.0 to obtain the radial Agrobacterium bacterial solution.

[0013] Furthermore, the preparation process of the polymyxa bacillus liquid is as follows: firstly, the polymyxa bacillus is streaked and inoculated to activate, and then a single colony is picked and inoculated into a liquid culture medium after 3 to 4 transfers, and then cultured at 37°C to 38°C and 150rpm to 160rpm until the OD 600 is 0.7-1.0, and a bacterial solution of Paenibacillus polymyxa is obtained.

[0014] The present invention also provides a method for preparing the biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema, comprising the following steps:

[0015] Weigh potassium humate, corkwood chips, citrus residues, and tobacco residues according to weight, air-dry them, and mix them to obtain a basic fertilizer for later use;

[0016] Preparation of microbial inoculants: LB liquid medium was used to culture radioactive Agrobacterium to OD600 When the OD value is 0.7 to 1, the radioactive Agrobacterium culture solution is obtained, and the polymyxa bacillus is cultured in LB liquid medium until the OD value is 600 When the value is equal to 0.7 to 1, the bacterial solution of Paenibacillus polymyxa is obtained;

[0017] Then, the Agrobacterium bacterial solution and the Paenibacillus polymyxa bacterial solution are evenly mixed in a volume ratio of 4-8:1-2, and then evenly sprayed into the mixed basic fertilizer according to weight portions, and stirred evenly to obtain organic fertilizer.

[0018] Furthermore, the strain number of the Agrobacterium radiobacterium is ACCC 10058, and the strain number of the Paenibacillus polymyxa is BNCC195313.

[0019] The present invention also provides an application of the biological organic fertilizer for preventing and controlling root rot of polygonatum sibiricum in the cultivation of polygonatum sibiricum, wherein the biological organic fertilizer is used for preventing and controlling root rot of polygonatum sibiricum, and the pathogen of the root rot of polygonatum sibiricum is Fusarium oxysporum or Fusarium solani.

[0020] Furthermore, the bio-organic fertilizer is used to promote the growth and development of Polygonatum cyrtonema.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] 1. The biological organic fertilizer prepared by the present invention can not only prevent and treat root rot of polygonatum cyrtonema caused by pathogenic bacteria Fusarium oxysporum and Fusarium solani, but also promote the growth and development of polygonatum cyrtonema; wherein the microbial agent can significantly improve the prevention and treatment effect of organic fertilizer on root rot of polygonatum cyrtonema. And it can promote the growth and development of polygonatum cyrtonema.

[0023] The radiobacterium and Paenibacillus polymyxa in the microbial agent act synergistically. Experiments have shown that radiobacterium has a growth-promoting effect on Paenibacillus polymyxa and promotes the proliferation of Paenibacillus polymyxa, thereby synergistically preventing and controlling root rot of Polygonatum cyrtonema. DETAILED DESCRIPTION

[0024] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments. Based on the embodiments in the present invention, all other embodiments obtained by ordinary technicians in this field without creative work belong to the scope of protection of the present invention. The experimental methods described in the embodiments of the present invention are conventional methods unless otherwise specified, and the materials, reagents, etc. used in the following embodiments can be obtained from commercial channels unless otherwise specified.

[0025] Example 1: A biological organic fertilizer for preventing and treating Polygonatum cyrtonema root rot and a preparation method thereof.

[0026] 1. Experimental Materials

[0027] 1. Test strains:

[0028] ACCC 10058, Agrobacterium radiobacter, purchased from Shanghai Aiyan Biotechnology Co., Ltd.;

[0029] Paenibacillus polymyxa (no. BNCC195313) was purchased from Henan Industrial Microbial Strain Engineering Technology Research Center;

[0030] 2. Raw materials:

[0031] The Phellodendron chinense sawdust was donated by Hunan Yangtze River Pharmaceutical Group.

[0032] Citrus pulp was purchased from Qingdao Yutaifeng Trading Co., Ltd.; potassium fulvic acid and tobacco residue were purchased from Nanning Herunfa Technology Co., Ltd.;

[0033] 2. Experimental Methods

[0034] 1. Preparation of biological organic fertilizer

[0035] (1) Preparation of microbial agents:

[0036] The preparation process of Agrobacterium radiobacterium culture liquid is as follows: Agrobacterium radiobacterium is streaked onto LB solid medium plates, transferred and purified 4 times, and a single colony is picked and inoculated into LB liquid medium, and cultured at 37°C and 180 rpm until OD 600 is 0.7, and a radioactive Agrobacterium bacterial solution is obtained.

[0037] The preparation process of the polymyxa bacillus liquid is as follows: first, the polymyxa bacillus is streaked onto an LB solid culture medium plate, transferred and purified for 4 times, a single colony is picked and inoculated into an LB liquid culture medium, and cultured at 37°C and 160 rpm until the OD 600 was 0.7, and the bacterial solution of Paenibacillus polymyxa was obtained.

[0038] Then, the bacterial solution of Agrobacterium radiobacterium and the bacterial solution of Paenibacillus polymyxa were mixed evenly in a volume ratio of 8:3 to obtain a microbial agent;

[0039] (2) Preparation of biological organic fertilizer:

[0040] Weigh 2kg of raw materials potassium humate, 3kg of Phellodendron sawdust, 2kg of citrus residue, and 2kg of cigarette residue, air-dry them naturally and mix them evenly to obtain basic fertilizer, then evenly spray 1kg of microbial agent into the basic fertilizer, stirring while spraying, and after evenly spraying, obtain biological organic fertilizer.

[0041] Example 2: A biological organic fertilizer for preventing and treating Polygonatum sibiricum root rot and a preparation method thereof.

[0042] The steps of Example 2 are basically the same as those of Example 1, except that:

[0043] The masses of the raw materials are: 1kg potassium humate, 2kg Phellodendron sawdust, 1kg citrus residue, 1kg tobacco residue, and 0.5kg microbial agent.

[0044] Example 3: A biological organic fertilizer for preventing and treating Polygonatum sibiricum root rot and a preparation method thereof.

[0045] The steps of Example 3 are basically the same as those of Example 1, except that:

[0046] The masses of the raw materials are: 3kg potassium humate, 4kg Phellodendron sawdust, 3kg citrus residue, 3kg tobacco residue, and 1.5kg microbial agent.

[0047] Comparative Example 1: A biological organic fertilizer and a preparation method thereof.

[0048] The steps are basically the same as those in Example 1, except that:

[0049] The microbial agent is a bacterial solution of Agrobacterium radiobacterium.

[0050] Comparative Example 2: A biological organic fertilizer and a preparation method thereof.

[0051] The steps are basically the same as those in Example 1, except that:

[0052] The microbial agent is a bacterial liquid of Paenibacillus polymyxa.

[0053] Comparative Example 3: A biological organic fertilizer and a preparation method thereof.

[0054] The steps are basically the same as those in Example 1, except that:

[0055] The microbial agent is obtained by uniformly mixing a bacterial solution of Agrobacterium radiobacterium and a bacterial solution of Paenibacillus polymyxa in a volume ratio of 3:8.

[0056] Comparative Example 4: A biological organic fertilizer and a preparation method thereof.

[0057] The steps are basically the same as those in Example 1, except that:

[0058] The microbial inoculant is obtained by uniformly mixing a bacterial solution of Agrobacterium radiobacterium and a bacterial solution of Paenibacillus polymyxa in a volume ratio of 6:5.

[0059] Comparative Example 5: A biological organic fertilizer and a preparation method thereof.

[0060] The steps are basically the same as those in Example 1, except that:

[0061] Use clean water instead of microbial agents.

[0062] Comparative Example 6: A biological organic fertilizer and a preparation method thereof.

[0063] The steps are basically the same as those in Example 1, except that:

[0064] Weigh 2kg of potassium humate, 4kg of Phellodendron sawdust, and 3kg of citrus residue, dry them naturally in the sun, and mix them evenly to obtain the basic fertilizer. (Do not add tobacco residue)

[0065] Comparative Example 7: A biological organic fertilizer and a preparation method thereof.

[0066] The steps are basically the same as those in Example 1, except that:

[0067] Weigh 2kg of potassium humate, 4kg of yellow cedar sawdust, and 3kg of tobacco residue, dry them naturally in the sun, and mix them evenly to obtain the basic fertilizer. (Do not add citrus residue)

[0068] Comparative Example 8: A biological organic fertilizer and a preparation method thereof.

[0069] The steps are basically the same as those in Example 1, except that:

[0070] Weigh 3kg of potassium humate, 3kg of citrus residue, and 3kg of tobacco residue, dry them naturally in the sun, and mix them evenly to obtain the basic fertilizer. (Do not add Phellodendron sawdust)

[0071] The effects of the bio-organic fertilizer prepared in the above embodiments and comparative examples on root rot of Polygonatum cyrtonema and its influence on the growth and development of Polygonatum cyrtonema were studied, and the specific process was as follows:

[0072] 1. Experimental Materials

[0073] 1. Polygonatum cyrtonema plant: A 2-year-old Polygonatum cyrtonema plant obtained through healthy sterile germination.

[0074] 2. Test pathogens: Fusarium redolens, Fusarium oxysporum and Fusarium solani.

[0075] Fusarium redolens is publicly recorded in the journal: Chen Yao, Shi Rongjiu, Zheng Liangcan, et al. Antibacterial effect of crude rhamnolipid extract on plant pathogenic fungi[J]. Journal of Ecology, 2022, 41(03): 520-527. DOI: 10.13292 / j.1000-4890.202202.035.

[0076] Fusarium oxysporum was purchased from Hunan Agricultural Microbiological Culture Collection Center, strain number 0412MF020120;

[0077] Fusarium solani is publicly recorded in the journal: Deng Xiaoxu, Xie Xia, Pan Yamei, et al. Screening and identification of antagonistic strains of Fusarium solani in tea trees and analysis of their growth-promoting and disease-preventing properties [J]. Tea Science, 2023, 43(01): 67-77. DOI: 10.13305 / j.cnki.jts.2023.01.005.

[0078] 2. Experimental Methods

[0079] 1. Pathogen inoculum

[0080] Fusarium redolens, Fusarium oxysporum and Fusarium solani were inoculated onto PDA plates respectively and cultured at 28°C for 3 days. A hole was punched on the PDA plate using a hole puncher, and about 5 bacterial cakes were picked out and put into 300 mL PDB culture medium, which were placed on a shaker and cultured at 28°C and 180 r / min for 3 days to obtain the inoculum of Fusarium redolens, the inoculum of Fusarium oxysporum and the inoculum of Fusarium solani, respectively.

[0081] The detection found that the concentration of the above pathogen inoculum was: the concentration of the fragrant Fusarium inoculum was 9ⅹ10 8 cfu / mL, the concentration of Fusarium oxysporum inoculum was 8ⅹ10 9 cfu / mL, the concentration of Fusarium solani inoculation solution was 6×10 8 cfu / mL.

[0082] 2. Prevention effect experiment

[0083] Polygonatum cyrtonema was cultivated with the biological organic fertilizer prepared by the present invention, and the control effects of Examples 1-3 and Comparative Examples 1-8 on root rot of Polygonatum cyrtonema were studied.

[0084] The experimental setup is as follows:

[0085] Control group (CK): only seedling soil, 5 kg seedling soil / pot, 12 pots per treatment, 1 plant / pot;

[0086] Experimental group: each pot was filled with a mixture of 4.95 kg of seedling soil + 50 g of biological organic fertilizer, 12 pots were treated in each treatment, and 1 plant was planted in each pot; the experimental groups were respectively recorded as Example 1, Example 2, Example 3, Comparative Example 1, Comparative Example 2 ... Comparative Example 8 according to the biological organic fertilizer.

[0087] Transplant the prepared Polygonatum cyrtonema plants into the above pots. After 7 days of transplanting, evenly pour the pathogen inoculum into the roots of the Polygonatum cyrtonema plants using the root irrigation method, pouring 10 mL of pathogen inoculum into each pot. Take samples after 20 days of cultivation, count the disease degree, calculate the disease index, and measure the total fresh weight of the plant and the fresh weight of the root.

[0088] The above experiments were carried out with the inoculum of Fusarium odoratum, Fusarium oxysporum and Fusarium solani, and the statistical results of the incidence were recorded.

[0089] The severity of root rot of Polygonatum cyrtonema is divided into 4 levels. Level 0 means that the root system is not diseased; Level 1 means that the root system disease rate is ≤25% and the leaves are normal; Level 2 means that the root system disease rate is 25%<≤50% and the leaves turn yellow; Level 3 means that the root system disease rate is 50%<≤75% and the leaves wilt; Level 4 means that the root system disease rate is more than 75% and the leaves are dry.

[0090] The disease index and control effect were calculated according to the following formulas:

[0091] Disease index = [Σ(number of diseased plants × representative value) / (total number of plants × highest diseased representative value)] × 100

[0092] Control effect = [(control disease index - treatment disease index) / control disease index] × 100%

[0093] 3. Experimental Results

[0094] Table 1 The preventive effect of the biological organic fertilizer of the present invention on the root rot of Polygonatum cyrtonema caused by Fusarium odoratum

[0095]

[0096]

[0097] As shown in Table 1, the bio-organic fertilizers prepared by the embodiments of the present invention and the comparative examples have certain control effects on the root rot of Polygonatum cyrtonema caused by Fusarium odoratum. The control effect of the embodiments of the present invention is 12.3-15.6%, and the control effect of comparative examples 1-8 is 2.6-9.9%. The promoting effect on the aboveground part is better than that on the underground part. Although the control effect of the embodiments of the present invention is higher than that of the comparative example, the effect is general. It is difficult to promote and apply in production.

[0098] Table 2 The preventive effect of the biological organic fertilizer of the present invention on the root rot of Polygonatum cyrtonema caused by Fusarium oxysporum

[0099] deal with Disease index Control effect (%) Fresh weight of stems and leaves (g / plant) Fresh root weight (g / plant) Control group 71.2a - 35.1a 16.2a Example 1 11.4c 84.0 105.4d 42.3d Example 2 12.8c 82.0 104.1d 40.1d Example 3 12.4c 82.6 102.7d 41.8d Comparative Example 1 17.1c 76.0 89.7c 38.8d Comparative Example 2 37.3b 47.6 51.2b 35.4c Comparative Example 3 30.1b 57.7 62.3b 36.9c Comparative Example 4 15.2c 78.7 77.8c 37.7c Comparative Example 5 40.4b 43.3 50.6b 33.2c Comparative Example 6 15.6c 78.1 78.8c 26.4b Comparative Example 7 16.8c 76.4 78.6c 27.1b Comparative Example 8 17.2c 75.8 75.7c 25.5b

[0100] According to Table 2, the biological organic fertilizer prepared by the method provided in Examples 1-3 of the present invention has a significant preventive effect on the root rot of Polygonatum cyrtonema caused by Fusarium oxysporum, reaching more than 82%; it can be seen from Comparative Examples 1-5 that when the radioactive Agrobacterium solution and the polymyxa bacillus solution in the prepared biological organic fertilizer microbial inoculant are mixed according to a volume ratio of 8:3, the preventive effect is optimal, when only the radioactive Agrobacterium solution is used, the preventive effect is reduced to 76%, and when only the polymyxa bacillus solution is used, the preventive effect (47.6%) of the prepared biological organic fertilizer is significantly lower than the preventive effect of using the radioactive Agrobacterium solution and Examples 1-3 alone. It can be seen from Comparative Examples 3 and 4 that the control effect is improved when the dosage of the radioactive Agrobacterium solution is increased and the dosage of the polymyxa Bacillus solution is reduced; however, when the dosage of polymyxa Bacillus is 0 (Comparative Example 1), the control effect of the biological organic fertilizer prepared therefrom is reduced compared with Examples 1-3 and Comparative Example 4, indicating that polymyxa Bacillus can promote the control effect of radioactive Agrobacterium on root rot of Polygonatum cyrtonema, which indicates that radioactive Agrobacterium is the main anti-disease bacterium, and polymyxa Bacillus may promote the growth of radioactive Agrobacterium to improve its resistance.

[0101] It can be seen from Comparative Examples 6-8 that among the raw materials of biological organic fertilizer, Phellodendron sawdust, citrus residue and tobacco residue all have the effect of promoting disease resistance.

[0102] In summary, the present invention uses Agrobacterium radiobacterium and Paenibacillus polymyxa to prepare a mixed bacterial agent, and when the amount of Agrobacterium radiobacterium is higher than that of Paenibacillus polymyxa, the preventive effect of the biological organic fertilizer on the root rot of Polygonatum cyrtonema caused by Fusarium oxysporum is significantly improved. In addition, the microbial agent and various raw material components in the embodiments of the present invention synergistically promote the fresh weight of the stems and leaves and the fresh weight of the roots of Polygonatum cyrtonema, thereby promoting the growth of Polygonatum cyrtonema.

[0103] Table 3 The preventive effect of the biological organic fertilizer of the present invention on the root rot of Polygonatum cyrtonema caused by Fusarium solani

[0104]

[0105]

[0106] As shown in Table 3, the biological organic fertilizer prepared in Examples 1-3 of the present invention has a significant preventive effect on the root rot of Polygonatum cyrtonema caused by Fusarium solani, with a preventive effect of more than 83.7%. By analyzing Comparative Example 1 and Comparative Examples 1-8, it can be seen that when the radioactive soil bacillus liquid and the polymyxa bacillus liquid in the prepared biological organic fertilizer microbial inoculant are mixed according to a volume ratio of 8:3, the prepared biological organic fertilizer has the highest preventive effect on the root rot of Polygonatum cyrtonema caused by Fusarium solani, and when the amount of radioactive soil bacillus is lower than that of polymyxa bacillus, its preventive effect is significantly reduced. The addition of Phellodendron sawdust, citrus residue and cigarette residue significantly improves the preventive effect of biological organic fertilizer on the root rot of Polygonatum cyrtonema, indicating that the present invention improves the preventive effect of the root rot of Polygonatum cyrtonema caused by Fusarium solani by combining the raw materials Phellodendron sawdust, citrus residue and cigarette residue. At the same time, it promotes the growth of Polygonatum cyrtonema.

[0107] In summary, the biological organic fertilizer prepared by the present invention can significantly prevent and control the root rot of Polygonatum cyrtonema caused by Fusarium oxysporum and Fusarium solani, and the prevention effect is more than 80%, but the prevention effect on the root rot of Polygonatum cyrtonema caused by Fusarium odoratum is not significant. Therefore, the biological organic fertilizer prepared by the present invention is suitable for large-scale prevention and control of the root rot of Polygonatum cyrtonema caused by Fusarium oxysporum and Fusarium solani.

[0108] Although the preferred embodiments of the present invention have been described, those skilled in the art may make additional changes and modifications to these embodiments once they have learned the basic creative concept. Therefore, the appended claims are intended to be interpreted as including the preferred embodiments and all changes and modifications that fall within the scope of the present invention.

[0109] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims

1. A biological organic fertilizer for preventing and treating Polygonatum cyrtonema root rot, characterized in that: The biological organic fertilizer is composed of the following components in parts by weight: 1 to 3 parts of potassium humate, 2 to 4 parts of yellow cedar sawdust, 1 to 3 parts of citrus residue, 1 to 3 parts of tobacco residue, and 0.5 to 1.5 parts of microbial agent; The microbial agent is composed of Agrobacterium radiobacter bacterial solution and Paenibacillus polymyxa bacterial solution in a volume ratio of 4-8:1-2.

2. The biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to claim 1, characterized in that: The microbial inoculant is composed of a bacterial solution of Agrobacterium radiobacterium and a bacterial solution of Paenibacillus polymyxa in a volume ratio of 6:

1.

3. The biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to claim 1, characterized in that: The biological organic fertilizer consists of the following components in parts by weight: 1 part of potassium humate, 4 parts of yellow cedar sawdust, 2 parts of citrus residues, 3 parts of tobacco residues, and 1 part of microbial agent.

4. The biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to claim 1, characterized in that: The biological organic fertilizer is composed of the following components in parts by weight: 2 parts of potassium humate, 3 parts of yellow cedar sawdust, 2 parts of citrus residues, 2 parts of tobacco residues, and 1.5 parts of microbial agent.

5. The biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to claim 1, characterized in that: The preparation process of the radioactive Agrobacterium bacterial solution is as follows: firstly, the radioactive Agrobacterium is streaked and inoculated to activate, and then a single colony is picked and inoculated into a liquid culture medium after transfer 3 to 4 times, and then cultured at 35° C. to 37° C. and 150 rpm to 180 rpm until the OD 600 is 0.7-1.0 to obtain a radioactive Agrobacterium bacterial solution.

6. The biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to claim 1, characterized in that: The preparation process of the polymyxa bacillus liquid is as follows: firstly, the polymyxa bacillus is streaked and inoculated to activate, and then a single colony is picked and inoculated into a liquid culture medium after 3-4 transfers, and then cultured at 37-38°C and 150-160 rpm until the OD 600 is 0.7-1.0, and a bacterial solution of Paenibacillus polymyxa is obtained.

7. A method for preparing a biological organic fertilizer for preventing and treating root rot of Polygonatum cyrtonema according to any one of claims 1 to 6, characterized in that: The steps include: Weigh potassium humate, corkwood chips, citrus residues, and tobacco residues according to weight, air-dry them, and mix them to obtain a basic fertilizer for later use; Preparation of microbial inoculants: LB liquid medium was used to culture radioactive Agrobacterium to OD 600 When the OD value is 0.7 to 1, the radioactive Agrobacterium culture solution is obtained, and the polymyxa bacillus is cultured in LB liquid medium until the OD value is 600 When the value is equal to 0.7 to 1, the bacterial solution of Paenibacillus polymyxa is obtained; Then, the bacterial solution of Agrobacterium radiobacterium and the bacterial solution of Paenibacillus polymyxa are evenly mixed in a volume ratio of 4-8:1-2, and then evenly sprayed into the mixed basic fertilizer according to weight portions, and stirred evenly to obtain organic fertilizer.

8. The method for preparing the biological organic fertilizer for preventing and treating root rot of polygonatum according to claim 7, wherein the radiobacterium strain is numbered as ACCC 10058, and the polymyxa bacillus is numbered as BNCC195313.

9. Use of the biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to any one of claims 1 to 6 in the cultivation of polygonatum cyrtonema, characterized in that: The biological organic fertilizer is used for preventing and treating root rot of polygonatum sibiricum, and the pathogen of the root rot of polygonatum sibiricum is Fusarium oxysporum or Fusarium solani.

10. The use of the biological organic fertilizer for preventing and treating root rot of polygonatum cyrtonema according to claim 9 in the planting of polygonatum cyrtonema, characterized in that: The biological organic fertilizer is used for promoting the growth and development of Polygonatum cyrtonema.

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