Introduced variety pig 10K SNP (Single Nucleotide Polymorphism) liquid phase chip based on targeted capture sequencing and application

By designing an imported pig 10K SNP liquid phase chip based on targeted capture sequencing, the problem of the existing high-cost pig SNP chip has been solved, cost reduction and selection efficiency improved, and it is suitable for early selection and subsequent genome analysis.

CN119331987AActive Publication Date: 2025-01-21SHIJI BIOTECHNOLOGY (NANJING) CO LTD +1
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Patent Information

Application Number
CN202411625082.9
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-14
Publication Date
2025-01-21
Estimated Expiration
2044-11-14

AI Technical Summary

Technical Problem

The number of existing pig SNP chip sites is more than 50K, which leads to high costs for breeding companies and is difficult to effectively reduce costs in early selection.

Method used

Design a type of pork 10K SNP liquid chip based on targeted capture sequencing, and prepare high -quality probes through collecting, screening and filtering target SNP sites to achieve chip preparation.

Benefits of technology

This chip can greatly reduce the cost of pig genomic selection, especially in the introduction of the early selection of breed pigs, and can ensure the selection efficiency and filling accuracy of the chip sites of more than 50K, and meet the needs of subsequent species identification and blood analysis.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses an introduced-variety pig 10K SNP liquid chip based on targeted capture sequencing and application, and belongs to the technical field of biomolecular breeding. Target SNP sites are collected, selected and filtered, an SNP site set with comprehensive performance is screened out, a probe is designed on the basis of the SNP site set, the introduced variety pig 10K SNP liquid phase chip is further prepared, the SNP sites of the chip have the characteristics of uniformity, polymorphism, compatibility, stability and functionality, pig genomes can be covered to the maximum extent, and the detection sensitivity is high. Meanwhile, the selection of pig genomes can be greatly reduced, especially the cost of early selection of variety pigs is reduced, in addition, the selection efficiency and filling accuracy of chip sites filled to more than 50K can be ensured, and the requirements of subsequent variety identification, lineage analysis, genetic relationship identification, genome selective breeding and whole genome association analysis are met.
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Citation Information

Patent Citations

  • Whole swine genome 50k SNP chip and application thereof

    CN110191965A

  • Pig 50K liquid phase chip based on targeted capture sequencing, and application thereof

    CN112921076A

  • Pig 10K liquid phase chip based on polysingle nucleotide polymorphism and application of pig 10K liquid phase chip

    CN116445584A

  • Preparation method and genetic typing method of 15K liquid phase chip for Min pig breeding

    CN117363750A

  • Pig liquid phase chip as well as preparation method and application thereof

    CN118086519A

Cited By

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    CN120967003A