Luminescence detection device, multi-detection index analysis system and detection method

Abstract

The invention relates to a luminescence detection device which comprises a detection module, an incubation module, a reaction cup transfer module, a liquid adding module, a liquid bearing module and a uniform mixing module, and the detection module is used for detecting an optical signal in a reaction cup; the incubation module is used for incubating the solution in the reaction cup; the reaction cup transfer module is used for transferring reaction cups; the liquid adding module is used for adding liquid required by detection into the reaction cup; the liquid bearing module is used for bearing at least one of substrate liquid, a sample and a reagent; the uniform mixing module is used for uniformly mixing the solution in the reaction cup. And the detection efficiency is effectively improved. The invention also relates to a multi-detection index analysis system and a detection method.

Description

Technical Field

[0001] The present invention relates to the technical field of in vitro diagnosis, and in particular to a luminescence detection device, a multi-detection index analysis system and a detection method. Background Art

[0002] At present, the commonly used methods and supporting instruments for detecting protein concentration in clinical practice are as follows:

[0003] 1. Latex enhanced immunoturbidimetry, which is usually used with a biochemical analyzer or a specific protein analyzer. This method is a homogeneous immunoassay with a fast detection speed and quantitative detection. However, this method has the following problems: low sensitivity, many low-abundance proteins cannot be tested with this method, and the linear range is narrow, which greatly limits its application.

[0004] 2. Enzyme-linked immunosorbent assay (ELISA): This method is usually tested with an ELISA reader. Since it requires multiple cleanings, the detection time is very long, the repeatability is very poor, and it is difficult to run automatically, its clinical application has become less and less.

[0005] 3. Fluorescence immunochromatography, usually used with a fluorescence immunochromatography analyzer. The instrument is small and easy to carry, so it is often used for point-of-care testing (POCT). However, it has poor repeatability, a narrow linear range, and low sensitivity. The detection time takes more than 10 minutes, and the detection throughput is low.

[0006] 4. Magnetic particle chemiluminescence method. This method has high sensitivity, wide linear range, and good repeatability, but requires magnetic separation and cleaning, which results in long detection time, complex and large equipment, low detection throughput, and high failure rate.

[0007] It can be seen that the above methods all have certain problems, such as being unable to simultaneously meet the requirements of high sensitivity, wide linear range, good repeatability, short detection time, and high detection throughput. At the same time, except for latex-enhanced immunoturbidimetry, other methods are difficult to integrate with biochemical analysis, blood cell analysis, and coagulation analysis, so it is difficult to meet the current popular trend of integrating detection items according to clinical needs.

[0008] Homogeneous chemiluminescence detection method, homogeneous chemiluminescence detection can simultaneously meet the requirements of high sensitivity, wide linear range, good repeatability, short detection time, and high detection throughput, and can well meet the current needs of clinical testing.

[0009] However, the detection equipment in the prior art cannot be used for automated homogeneous chemiluminescence detection and requires manual or assisted manual operation, resulting in low operating efficiency, large operating errors, slow test speed, easy errors, and difficulty in integration into biochemical analysis, blood cell analysis, and coagulation analysis platforms. Therefore, there is an urgent need for an automated detection device that can meet the requirements of homogeneous luminescence and is compatible with existing biochemical analysis, blood cell analysis, and coagulation analysis platforms. Summary of the invention

[0010] An object of the present invention is to provide a luminescence detection device to solve at least one of the above-mentioned technical problems.

[0011] To achieve the above-mentioned object, the present invention provides a luminescence detection device in a first aspect, comprising a detection module, wherein the detection module is used to detect an optical signal in a reaction cup;

[0012] An incubation module, wherein the incubation module is used to incubate the solution in the reaction cup;

[0013] A reaction cup transfer module, wherein the reaction cup transfer module is used to transfer the reaction cup;

[0014] A liquid adding module, which is used to add liquid required for detection into the reaction cup;

[0015] a liquid carrying module, the liquid carrying module being used to carry at least one of a substrate liquid, a sample and a reagent; and

[0016] A mixing module is used to mix the solution in the reaction cup.

[0017] Preferably, the liquid adding module is movable in three-dimensional space.

[0018] Preferably, the mixing module mixes the solution in the reaction cup by moving it.

[0019] Preferably, the mixing module includes at least one of an ultrasonic mixing submodule, a vibration mixing submodule and a rotation mixing submodule.

[0020] Preferably, when the mixing module includes the rotation mixing submodule, the rotation mixing submodule is connected to the reaction cup transfer module.

[0021] Preferably, at least one of the liquid adding module and the liquid carrying module is movable so as to transfer the substrate liquid, sample and / or reagent in the liquid carrying module.

[0022] Preferably, the liquid adding module and the liquid carrying module are capable of relative translation and relative vertical movement.

[0023] Preferably, the liquid-bearing module can move in translation and / or in a vertical direction, and the liquid-adding module can move in a vertical direction and / or in translation.

[0024] Preferably, the liquid adding module comprises a pipetting submodule and / or a substrate loading submodule, wherein the pipetting submodule is used for loading samples and / or reagents into the reaction cup; and the substrate loading submodule is used for loading substrate liquid into the reaction cup.

[0025] Preferably, the pipetting submodule and / or the substrate loading submodule can be moved to the top of the reaction cup, and / or the reaction cup transfer module can transfer the reaction cup to the bottom of the pipetting submodule and / or the substrate loading submodule; and / or the pipetting submodule and / or the substrate loading submodule can be moved to the top of the liquid carrying module.

[0026] Preferably, the pipetting submodule and / or substrate loading submodule comprises a pipetting needle, and the pipetting needle is capable of aspirating and releasing substrate liquid, sample and / or reagent.

[0027] Preferably, the liquid-carrying module comprises a sample tube, and the pipette needle can pass through a tube cap of the sample tube to absorb the sample.

[0028] Preferably, the pipetting submodule and / or the substrate loading submodule comprises a liquid driving unit for driving the liquid to flow.

[0029] Preferably, the liquid driving unit is a pump or a syringe.

[0030] Preferably, the luminescence detection device further comprises a washing module, and the washing module is used for washing the liquid adding module and / or the stirring module.

[0031] Preferably, the liquid adding module can be moved to the washing module, or the washing module can be moved to the liquid adding module.

[0032] Preferably, the reaction cup transfer module picks up the reaction cup by lifting, clamping and / or adsorption; and / or the reaction cup transfer module can transfer the reaction cup in three-dimensional space; and / or the reaction cup transfer module can rotate.

[0033] Preferably, the reaction cup transfer module is capable of transferring the reaction cup to the incubation module.

[0034] Preferably, the incubation module comprises an incubation tray, and the incubation tray is used to carry the reaction cup.

[0035] Preferably, the incubation tray is rotatable.

[0036] Preferably, the incubation module further comprises an incubation tray driving submodule, and the incubation tray driving submodule is used for driving the incubation tray to rotate.

[0037] Preferably, the incubation tray driving submodule comprises a driving member, a driving gear and a driven gear, wherein the driving member is connected to the driving gear and is used to drive the driving gear to rotate, and the driven gear is connected to the incubation tray and meshes with the driven gear.

[0038] Preferably, the incubation tray is annular, and the reaction cup transfer module is arranged in the inner circle or above the ring.

[0039] Preferably, the detection module is disposed inside or outside the incubation module.

[0040] Preferably, the incubation tray is also used to control the temperature of the reaction cup to be constant.

[0041] Preferably, the luminescence detection device further comprises a refrigeration module, and the refrigeration module is used to refrigerate at least one of the samples, reagents and substrates.

[0042] Preferably, the detection module comprises a darkroom and a detection submodule, the darkroom can accommodate a reaction cup, and the detection submodule is used to detect the optical signal in the reaction cup in the darkroom.

[0043] Preferably, the detection submodule includes a photomultiplier tube.

[0044] Preferably, the liquid carrying module comprises at least one of a sample carrying portion, a reagent chamber and a substrate liquid chamber, the sample carrying portion is used to carry samples, the reagent chamber is used to carry reagents, and the substrate liquid chamber is used to carry substrate liquid.

[0045] Preferably, the sample carrying portion is an annular structure, and the reagent chamber and the substrate liquid chamber are arranged inside or outside the annular structure.

[0046] Preferably, the luminescence detection device further comprises a cuvette storage module and / or a cuvette recovery module, wherein the cuvette storage module is used to store unused cuvettes, and the cuvette recovery module is used to recover used cuvettes.

[0047] Preferably, the reaction cup transfer module can pick up the reaction cup in the reaction cup storage module, or the reaction cup storage module can transfer the stored reaction cup to a preset position, the reaction cup transfer module can pick up the reaction cup from the preset position, and / or the reaction cup transfer module can transfer the reaction cup to the reaction cup recovery module.

[0048] Preferably, the reaction cup storage module comprises a storage submodule and a reaction cup arrangement submodule, the storage submodule is used to store reaction cups and provide reaction cups to the reaction cup arrangement submodule, and the reaction cup arrangement submodule is used to arrange the reaction cups according to a preset arrangement rule.

[0049] Preferably, the liquid adding module, the detection module, the reaction cup storage module and / or the reaction cup recovery module are arranged on the side, upper part or lower part of the incubation plate.

[0050] Preferably, the reaction cup transfer module includes a first moving component, a second moving component and a reaction cup clamping component, the execution end of the first moving component can move along a first direction, the second moving component is connected to the execution end of the first moving component so that the second moving component can move along the first direction; the execution end of the second moving component can move along a second direction different from the first direction, and the reaction cup clamping component is connected to the execution end of the second moving component so that the reaction cup clamping component can move along the second direction.

[0051] Preferably, the reaction cup recovery module is located on the moving track of the reaction cup transfer module, and the detected reaction cup is transferred to the reaction cup recovery module through the reaction cup transfer module.

[0052] Preferably, the reaction cup recovery module comprises:

[0053] a recovery track, the entrance of which is located on the moving track of the execution end of the reaction cup transfer module; and

[0054] A recovery box is arranged at the exit of the recovery track.

[0055] Preferably, the reaction cup arrangement submodule comprises an inclined arrangement channel, and the upper end of the arrangement channel is connected to the storage submodule for receiving the reaction cups output from the storage submodule.

[0056] Preferably, the luminescence detection device further comprises a reaction cup temporary storage module, and the reaction cup temporary storage module is arranged at the exit of the reaction cup arrangement submodule and is located on the moving track of the reaction cup transfer module.

[0057] Preferably, the reaction cup temporary storage module includes a reaction cup temporary storage seat arranged at the exit of the reaction cup arrangement submodule.

[0058] Preferably, the reaction cup temporary storage module may further include a first motor, and the first motor is used to drive the reaction cup temporary storage seat to rotate so as to align the reaction cup temporary storage groove with the outlet of the reaction cup arrangement submodule.

[0059] Preferably, the mixing module is located on the moving track of the reaction cup transfer module.

[0060] Preferably, the liquid carrying portion comprises a sample carrying portion, a reagent chamber and a substrate liquid chamber, the sample carrying portion is used to carry samples, the reagent chamber is used to carry reagents, and the substrate liquid chamber is used to carry substrate liquid.

[0061] Preferably, the reagent compartment includes a reagent table, on which a reagent placement portion is provided, and a reagent bottle containing a reagent can be placed in the reagent placement portion; the sample carrying portion is provided with a sample rack, and the sample rack is used to carry a sample tube.

[0062] Preferably, the sample rack is a linear structure.

[0063] Preferably, the liquid adding module includes a pipetting submodule and a substrate loading submodule, the pipetting submodule includes a sample loading unit and a reagent loading unit, the sample loading unit is used to load samples into the reaction cup, the reagent loading unit is used to load reagents into the reaction cup, and the substrate loading submodule is used to load substrate liquid into the reaction cup.

[0064] Preferably, the sample loading unit comprises a sample pipetting needle, the second lifting component for driving the sample pipetting needle to move up and down, and a translation component for driving the sample pipetting needle to move in translation.

[0065] Preferably, the reagent loading unit includes a reagent pipetting needle, a first lifting component for driving the reagent pipetting needle to rise and fall, and a rotating component for driving the reagent pipetting needle to rotate.

[0066] Preferably, the luminescence detection device further comprises a washing module, and the washing module comprises a reagent washing submodule for washing the reagent loading unit and a sample washing submodule for washing the sample loading unit.

[0067] Preferably, the reaction cup transfer module includes a first rotating drive member, a first rotating arm, a second rotating drive member, a second rotating arm and a pick-and-place member connected in sequence, the first rotating drive member drives the first rotating arm to rotate, the second rotating drive member drives the second rotating arm to rotate, and the pick-and-place member realizes the lifting, clamping and / or adsorption functions.

[0068] Another object of the present invention is to provide a multi-detection index analysis system, comprising at least one of a biochemical analysis module, a blood cell analysis module and a blood coagulation analysis module and the above-mentioned luminescence detection device;

[0069] When the multi-detection index analysis system includes at least one of the liquid adding module, the liquid carrying module, the incubation module and the washing module and the biochemical analysis module, the biochemical analysis module matches and uses at least one of the liquid adding module, the liquid carrying module, the incubation module and the washing module of the luminescence detection device;

[0070] When the multi-detection index analysis system includes at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module, the recovery module, the reaction cup storage module and the washing module and the blood coagulation analysis module, the blood coagulation analysis module matches and uses at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module, the recovery module, the reaction cup storage module and the washing module of the luminescent detection device;

[0071] When the multi-detection index analysis system includes at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module and the washing module and the blood cell analysis module, the blood cell analysis module matches and uses at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module and the washing module of the luminescence detection device.

[0072] Preferably, when the multi-detection index analysis system includes the biochemical analysis module, the incubation module includes an outer incubation tray and an inner incubation tray, the outer incubation tray is an annular structure, the reaction cup carried by the outer incubation tray contains one of the solution to be biochemically analyzed and the solution to be homogeneously luminescent detected, and the reaction cup carried by the outer incubation tray contains another of the solution to be biochemically analyzed and the solution to be homogeneously luminescent detected.

[0073] Preferably, the outer incubation tray and / or the inner incubation tray are rotatable.

[0074] Another object of the present invention is to provide a detection method to solve at least one of the above technical problems.

[0075] To achieve this purpose, the third aspect of the present invention adopts the following technical solution:

[0076] A detection method, the luminescence detection device performs the detection method, the detection method comprising:

[0077] Detection: Place the reaction cup on the detection module, and the detection module detects the test liquid in the reaction cup.

[0078] Optionally, before the detection, the method further includes:

[0079] Preparation of the test liquid: The liquid adding module adds the liquid required for the test into the reaction cup, so that the liquid forms the test liquid in the reaction cup.

[0080] Optionally, before the detection of the test liquid, the method includes:

[0081] Transferring the reaction cup: the reaction cup transfer module or the incubation module transfers the reaction cup to the detection module.

[0082] Optionally, the step of preparing the test solution comprises:

[0083] Adding sample: the liquid adding module adds the sample to the reaction cup; and / or,

[0084] Adding reagents: the liquid adding module adds reagents to the reaction cup; and / or,

[0085] Adding substrate liquid: the liquid adding module adds the substrate liquid into the reaction cup.

[0086] Optionally, after adding the reagent and / or after adding the substrate solution, the method further comprises:

[0087] Mixing: The mixing module mixes the liquid in the reaction cup;

[0088] and / or,

[0089] After adding the sample and / or after adding the reagent and / or after adding the substrate solution, the method further comprises:

[0090] Cleaning: The washing module cleans the liquid adding module and / or the stirring module.

[0091] Optionally, before adding the sample, the step further includes:

[0092] Taking samples: the liquid adding module moves to the liquid carrying module, and / or the liquid carrying module moves to the liquid adding module, and the liquid adding module absorbs the sample in the liquid carrying module;

[0093] And / or before adding the reagent, the method further comprises:

[0094] Taking reagents: the liquid adding module moves to the liquid carrying module, and / or the liquid carrying module moves to the liquid adding module, and the liquid adding module absorbs the reagents in the liquid carrying module.

[0095] Optionally, the adding sample includes:

[0096] The reaction cup transfer module transfers the reaction cup to the bottom of the liquid adding module; or the liquid adding module moves to the top of the reaction cup, and the liquid adding module adds the sample to the reaction cup; or the incubation module transfers the reaction cup to the bottom of the liquid adding module; and / or

[0097] The reagent addition comprises:

[0098] The reaction cup transfer module transfers the reaction cup to the bottom of the liquid adding module; or the liquid adding module moves to the top of the reaction cup, and the liquid adding module adds the reagent to the reaction cup; the incubation module transfers the reaction cup to the bottom of the liquid adding module; and / or

[0099] The substrate liquid addition comprises:

[0100] The reaction cup transfer module transfers the reaction cup to the bottom of the liquid adding module; or the liquid adding module moves to the top of the reaction cup, and the liquid adding module adds the substrate liquid to the reaction cup; the incubation module transfers the reaction cup to the bottom of the liquid adding module.

[0101] Optionally, the detection further includes:

[0102] Incubation: The cuvette is incubated at the preset temperature of the incubation module for the preset time.

[0103] Optionally, before the detection, the method further includes:

[0104] Providing a reaction cup: the reaction cup transfer module picks up the reaction cup provided by the reaction cup storage module or the reaction cup storage module provides the reaction cup to the incubation module;

[0105] And / or, the detection further includes:

[0106] Recycling reaction cup: The reaction cup transfer module transfers the reaction cup to the reaction cup recycling module.

[0107] It can be seen from the above that the technical solution provided by the present invention is

[0108] The luminescence detection device has a reasonable structure and is used for homogeneous chemiluminescence detection. It can simultaneously meet the requirements of high sensitivity, wide linear range, good repeatability, short detection time, high detection throughput, and small instrument size. It can well meet the needs of current clinical detection.

[0109] In the multi-detection index analysis system, the chemical analysis module, the blood cell analysis module and the blood coagulation analysis module can match and use some modules of the luminescence detection device, thereby simplifying the structure of the multi-detection index analysis system. At least one of the chemical analysis module, the blood cell analysis module and the blood coagulation analysis module can be integrated with the luminescence detection device, so that while realizing the luminescence analysis, at least one of the biochemical analysis, blood cell analysis and blood coagulation analysis can be realized, thereby increasing the number of indicators detected by the multi-detection index analysis system and improving the detection efficiency.

[0110] The detection method is simple and the steps are reasonable, which can improve the detection efficiency and detection accuracy. BRIEF DESCRIPTION OF THE DRAWINGS

[0111] Figure 1is a schematic diagram of the working process of the luminescence detection device provided by an embodiment of the present invention;

[0112] Figure 2 is a schematic structural diagram of a luminescence detection device provided in an embodiment of the present invention;

[0113] Figure 3 It is a structural schematic diagram of a multi-detection index analysis system provided by an embodiment of the present invention;

[0114] Figure 4 is a structural schematic diagram of another multi-detection index analysis system provided by an embodiment of the present invention;

[0115] Figure 5a is a schematic structural diagram of a pick-and-place component and a mixing module provided in an embodiment of the present invention;

[0116] Figure 5b is a schematic diagram of the movement process of the sample tube when the mixing module provided by the embodiment of the present invention is mixing;

[0117] Figure 6 It is a structural schematic diagram of a reaction cup storage module and a reaction cup arrangement submodule provided in an embodiment of the present invention;

[0118] Figure 7 is a structural schematic diagram of a reaction cup recovery module provided by an embodiment of the present invention;

[0119] Figure 8 is a schematic structural diagram of a washing module provided in an embodiment of the present invention;

[0120] Fig. 9 is a flow chart of a detection method provided by an embodiment of the present invention;

[0121] Fig.10 is a perspective view of a luminescence detection device provided by an embodiment of the present invention from a first viewing angle;

[0122] Fig.11 is a perspective view of a luminescence detection device provided by an embodiment of the present invention at a second viewing angle;

[0123] Fig.12 is a perspective view of the luminescence detection device provided by an embodiment of the present invention from a third viewing angle;

[0124] Fig.13 is a perspective view of a luminescence detection device provided by an embodiment of the present invention at a fourth viewing angle;

[0125] Fig.14 It is a top view of the luminescence detection device provided by an embodiment of the present invention.

[0126] In the figure:

[0127] 1. Liquid adding module; 11. Liquid transfer submodule; 111. Liquid transfer needle; 1111. Reagent liquid transfer needle; 1112. Sample liquid transfer needle; 112. Sample loading unit; 1121. Second lifting assembly; 1122. Translation assembly; 113. Reagent loading unit; 1131. First lifting assembly; 1132. Rotation assembly; 12. Substrate loading submodule;

[0128] 3. Detection module; 31. Darkroom; 32. Detection submodule;

[0129] 4. Mixing module; 41. Mixing seat; 42. Mixing motor;

[0130] 5. Liquid carrying module; 51. Sample carrying part; 511. Sample rack; 512. Sample tray; 52. Sample tube; 521. Tube cap; 53. Reagent compartment; 531. Reagent table; 532. Reagent placement part; 54. Substrate liquid compartment;

[0131] 6. Cuvette transfer module; 61. First rotating arm; 62. Second rotating arm; 63. Pick-and-place component; 64. First moving assembly; 65. Second moving assembly; 66. Cuvette clamping assembly;

[0132] 7. Washing module; 71. Reagent washing submodule; 72. Sample washing submodule;

[0133] 8. Incubation module; 81. Incubation tray; 811. External incubation tray; 812. Internal incubation tray; 82. Incubation position; 83. Incubation tray drive submodule; 831. Active gear; 832. Driven gear;

[0134] 9. Reaction cup storage module; 91. Storage submodule; 911. Lifting bottom plate; 92. Reaction cup arrangement submodule; 921. Arrangement channel;

[0135] 10. Reaction cup recovery module; 10a. Recovery track; 10b. Recovery box;

[0136] 20. Biochemical analysis module; 30. Blood coagulation analysis module; 40. Stirring module; 50. Biochemical reaction cup cleaning module; 60. Reagent adding position; 70. Sample adding position;

[0137] 80. Reaction cup temporary storage module; 801. Reaction cup temporary storage seat; 802. First motor; 100. Reaction cup; 101. Biochemical reaction cup; 102. Luminous reaction cup. DETAILED DESCRIPTION

[0138] The technical solution of the present invention is further described below in conjunction with the accompanying drawings and through specific implementation methods. It is understood that the specific embodiments described herein are only used to explain the present invention, rather than to limit the present invention. It is also necessary to explain that, for ease of description, only the parts related to the present invention are shown in the accompanying drawings, rather than all.

[0139] Some directional words are defined in the present invention. Unless otherwise specified, the directional words used, such as "up", "down", "left", "right", "inside" and "outside", are used to facilitate understanding and therefore do not constitute a limitation on the scope of protection of the present invention.

[0140] In the present invention, unless otherwise clearly specified and limited, a first feature being "above" or "below" a second feature may include that the first and second features are in direct contact, or may include that the first and second features are not in direct contact but are in contact through another feature between them. Moreover, a first feature being "above", "above" and "above" a second feature includes that the first feature is directly above and obliquely above the second feature, or simply indicates that the first feature is higher in level than the second feature. A first feature being "below", "below" and "below" a second feature includes that the first feature is directly below and obliquely below the second feature, or simply indicates that the first feature is lower in level than the second feature.

[0141] In the description of the present invention, unless otherwise clearly specified and limited, the terms "connected", "connected", and "fixed" should be understood in a broad sense, for example, it can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be a direct connection or an indirect connection through an intermediate medium, it can be the internal connection of two elements or the interaction relationship between two elements. For ordinary technicians in this field, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0142] Embodiment 1

[0143] This embodiment provides a luminescence detection device. Exemplarily, the luminescence detection device can be a homogeneous luminescence detection device or a fluorescence detection device. The luminescence detection device can be used for PCT detection in whole blood, but is not limited to this and can also be used for other detections.

[0144] like Figure 1-Figure 3As shown, the luminescence detection device provided in this embodiment includes a detection module 3, and the detection module 3 is used to detect the optical signal in the reaction cup 100. The reaction cup 100 can contain a liquid to be tested, and the light is emitted from the liquid to be tested. Exemplarily, the light can be chemiluminescence or fluorescence. In addition, when classified according to the duration of luminescence, the light can be glow or flash, etc. The detection module 3 can specifically detect the intensity of light. Optionally, the detection module 3 can detect the intensity of light in the reaction cup 100, that is, detect the light intensity signal in the reaction cup 100, and then obtain the content of the analyte in the liquid to be tested through the light intensity signal. For example, the PCT content in the sample is calculated based on the optical signal data, the hemoglobin concentration of the sample, and the hematocrit.

[0145] Optionally, the detection module 3 includes a darkroom 31 and a detection submodule 32. The darkroom 31 can accommodate the reaction cup 100, and the darkroom 31 can provide an environment required for detection, such as the environment in the darkroom 31 is darker than the external environment. More preferably, at least during detection, natural light from the outside cannot enter the darkroom 31. The detection submodule 32 is used to detect the optical signal in the reaction cup 100 in the darkroom 31. The detection submodule 32 includes a photomultiplier tube (i.e., a PMT detector), a photodiode (PD), an avalanche photodiode (APD), a silicon photomultiplier tube (SiPM), a complementary metal oxide semiconductor (CMOS) or a charge coupled device (CCD).

[0146] Optionally, the test liquid contained in the reaction cup 100 may be a mixture of a sample, a reagent and a substrate liquid.

[0147] Optionally, in order to provide at least one of a sample, a reagent and a substrate solution to the reaction cup 100 , the luminescence detection device may further include a liquid carrying module 5 , and the liquid carrying module 5 is used to carry at least one of a sample, a reagent and a substrate solution.

[0148] like Figure 1-Figure 3 As shown, illustratively, the liquid carrying module 5 includes at least one of a sample carrying portion 51, a reagent compartment 53, and a substrate liquid compartment 54. The sample carrying portion 51 is used to carry samples, the reagent compartment 53 is used to carry reagents, and the substrate liquid compartment 54 is used to carry substrate liquid. Optionally, the reagent compartment 53 may have a refrigeration function to store reagents at a preset temperature.

[0149] Exemplarily, the sample holding portion 51 includes a sample rack 511 (eg, Figure 2 , Figure 4 ) or sample tray 512 (as shown Figure 3 As shown), the sample rack 511 can be a rack or box with an opening at the upper end, as shown in FIG. Figure 3As shown, the sample tray 512 can be an annular structure or a circular structure. When the sample tray 512 is an annular structure, the reagent compartment 53 and the substrate liquid compartment 54 are arranged inside the annular structure, that is, arranged in the inner circle of the annular structure. Of course, the relative positions between the sample carrying portion 51, the reagent compartment 53 and the substrate liquid compartment 54 are not limited thereto, and the reagent compartment 53 and the substrate liquid compartment 54 can also be arranged outside the annular structure, such as arranged on the upper side, the lower side or one side of the horizontal direction of the annular structure.

[0150] Exemplarily, the sample rack 511 or the sample tray 512 can move in three-dimensional space or two-dimensional space, such as, specifically, the sample rack 511 or the sample tray 512 moving in two-dimensional space can include the sample rack 511 or the sample tray 512 translating in a horizontal plane or the like, such as moving forward, backward, left, and right, and the sample tray 512 can also rotate in a horizontal plane. The sample rack 511 or the sample tray 512 moving in three-dimensional space can include the sample rack 511 or the sample tray 512 moving in a horizontal space and in a vertical direction, such as the sample rack 511 or the sample tray 512 moving in forward, backward, left, right, and vertical directions, and / or rotating in a horizontal plane.

[0151] The sample rack 511 and the sample tray 512 can both be used to place samples. Optionally, the liquid carrying module 5 may include a sample tube 52. The sample rack 511 and / or the sample tray 512 can both be used to place the sample tube 52, which can contain the sample. The sample tube 52 can be covered with a tube cap 521 to prevent sample contamination.

[0152] In order to keep the sample, reagent and / or substrate at a preset temperature, such as a relatively low temperature and lower than the ambient temperature, the luminescence detection device may optionally further include a refrigeration module, which is used to refrigerate at least one of the sample, reagent and substrate. For example, part of the sample, reagent and / or substrate may be refrigerated in the refrigeration module; for another example, a refrigeration module may be provided outside the sample carrier 51, the reagent compartment 53 and / or the substrate liquid compartment 54.

[0153] Optionally, in order to facilitate the transfer of the sample, reagent and / or substrate liquid in the liquid carrying module 5 to the reaction cup 100, the luminescence detection device may further include a liquid adding module 1, and the liquid adding module 1 is used to add the liquid required for detection to the reaction cup 100. Furthermore, the liquid adding module 1 is used to transfer the liquid in the liquid carrying module 5 to the reaction cup 100. It can be understood that the liquid can be at least one of the sample, reagent and substrate liquid.

[0154] Optionally, the liquid adding module 1 can be moved in three-dimensional space, for example, the liquid adding module 1 can be moved to the sample carrying portion 51, the reagent chamber 53 and / or the substrate liquid chamber 54, and absorb the liquid in the sample carrying portion 51, the reagent chamber 53 and / or the substrate liquid chamber 54; for another example, the liquid adding module 1 can be moved to the reaction cup 100 to which liquid needs to be added, and add the liquid absorbed by the liquid adding module 1 to the reaction cup 100.

[0155] Optionally, at least one of the liquid adding module 1 and the liquid carrying module 5 can be moved so that the liquid adding module 1 can move the substrate liquid, sample and / or reagent in the liquid carrying module 5. For example, the liquid carrying module 5 does not move, and the liquid adding module 1 can at least move between the liquid carrying module 5 and the reaction cup 100 to be added with liquid; or, the liquid adding module 1 does not move, and the liquid carrying module 5 can move to the liquid adding module 1 so that the liquid adding module 1 can absorb the required liquid (the liquid can be at least one of the sample, reagent or substrate liquid); or both the liquid adding module 1 and the liquid carrying module 5 can move.

[0156] like Figure 1 As shown, in a specific embodiment, the liquid adding module 1 and the liquid carrying module 5 can move relatively in translation and in vertical direction. Optionally, the liquid carrying module 5 can move in translation and / or in vertical direction, and the liquid adding module 1 can move in vertical direction and / or in translation. For example, the liquid carrying module 5 can move in translation, and the liquid adding module 1 can move in vertical direction. For example, the liquid carrying module 5 moves to the bottom of the liquid adding module 1, and the liquid adding module 1 can absorb the liquid in the liquid carrying module 5 when it moves downward. Of course, in other optional embodiments, the liquid adding module 1 can be translated, and the liquid carrying module 5 can be moved in the vertical direction; or both the liquid adding module 1 and the liquid carrying module 5 can be translated, and at the same time, the liquid adding module 1 can also be moved in the vertical direction; or, both the liquid adding module 1 and the liquid carrying module 5 can be translated, and at the same time, the liquid adding module 1 can also be moved in the vertical direction; or, both the liquid adding module 1 and the liquid carrying module 5 can be moved in the vertical direction, and at the same time, the liquid carrying module 5 can also be translated; or, both the liquid carrying module 5 and the liquid adding module 1 can move in the vertical direction and translate.

[0157] like Figure 2-Figure 4 As shown, in an optional embodiment, the liquid adding module 1 includes a liquid transfer submodule 11 (such as Figure 4As shown in the figure, the pipetting submodule 11 is used to load samples and / or reagents into the reaction cup 100. Exemplarily, the number of the pipetting submodule 11 can be one, and the pipetting submodule 11 can transfer samples and reagents respectively. In order to ensure the purity of various samples and reagents and avoid mutual contamination between the two, the pipetting submodule 11 can be cleaned after transferring one liquid and before transferring another liquid. For example, after the pipetting submodule 11 transfers the sample in the sample holding part 51 to the reaction cup 100, the pipetting submodule 11 is cleaned, and then the pipetting submodule 11 transfers the reagent chamber 53 (as shown in the figure) to the reaction cup 100. Figure 2-Figure 4 ) is transferred to the reaction cup 100. Figure 2-Figure 3 As shown, of course, the number of the pipetting submodules 11 can also be two or more, and the pipetting submodule 11 can include a sample loading unit 112 and a reagent loading unit 113. The number of the sample loading unit 112 can be one or more, and the number of the reagent loading unit 113 can be one or more. The sample loading unit 112 is used to transfer the sample in the sample holding portion 51 to the reaction cup 100, and the reagent loading unit 113 is used to transfer the reagent in the reagent compartment 53 to the reaction cup 100.

[0158] The pipetting submodule 11 may further include a substrate loading submodule 12 , and the substrate loading submodule 12 is used to load substrate liquid into the reaction cup 100 .

[0159] like Figure 2-Figure 4 As shown, there may be a certain space between the substrate loading submodule 12, the sample loading unit 112 and the reagent loading unit 113, and the pipetting submodule 11 and / or the substrate loading submodule 12 may be respectively moved to the top of the liquid carrying module 5. The pipetting submodule 11 and / or the substrate loading submodule 12 may be respectively moved to the top of the reaction cup 100, and / or the reaction cup transfer module 6 (described in detail below) may transfer the reaction cup 100 to the bottom of the pipetting submodule 11 and / or the substrate loading submodule 12.

[0160] Optionally, the pipetting submodule 11 (sample loading unit 112 and / or reagent loading unit 113) and / or the substrate loading submodule 12 may include a pipetting needle 111, which can absorb and release substrate liquid, sample and / or reagent. Figure 1 As shown, optionally, the pipette needle 111 can penetrate the tube cap 521 of the sample tube 52 to absorb the sample, such as the pipette needle 111 descends below the sample liquid surface to absorb the sample. Exemplarily, the pipette needle 111 descends 2-5 mm below the sample liquid surface to absorb the sample.

[0161] like Figure 1 and Figure 2As shown, the liquid transfer submodule 11 (sample loading unit 112 and / or reagent loading unit 113) and / or substrate loading submodule 12 includes a liquid drive unit for driving the flow of liquid, and the liquid drive unit can be a pump or a syringe, and the pump can be connected to the pipette needle 111 or other liquid inlet and outlet parts, so as to drive the liquid to flow into the pipette needle 111 or drive the liquid to flow out of the pipette needle 111. The syringe can automatically or manually suck up and release the liquid.

[0162] Optionally, the pipetting submodule 11 and / or the substrate loading submodule 12 can transfer the liquid in the liquid carrying module 5 to the reaction cup 100 according to the amount required for detection.

[0163] like Figure 2 and Figure 8 As shown, the luminescence detection device may further include a washing module 7, and the washing module 7 is used to wash the liquid adding module 1 and / or the stirring module 40. Optionally, the washing module 7 is arranged on one side of the sample loading unit 112. Optionally, in this embodiment, one side or side portion may be an upper side, a lower side or a side in the horizontal direction, the upper side may be directly above or above the side, and the lower side may be directly below or below the side. Optionally, the liquid adding module 1 may be moved to the washing module 7, or the washing module 7 may be moved to the liquid adding module 1, so as to wash the liquid adding module 1.

[0164] Optionally, the washing module 7 can wash the pipette needle 111, and in particular, the washing module 7 can wash the pipette needle 111 of the pipette submodule 11 (sample loading unit 112 and / or reagent loading unit 113), so as to avoid cross contamination between samples. Exemplarily, the washing module 7 can wash the outside of the pipette needle 111 and the liquid channel of the pipette needle 111 (the liquid channel is a space opened inside the pipette needle 111 for accommodating liquid), such as the pipette needle 111 can be repeatedly aspirated and released by the washing liquid of the washing module 7 to clean the pipette needle 111.

[0165] like Figure 1 and Figure 5a As shown, optionally, the luminescence detection device may further include a mixing module 4, and the mixing module 4 is used to mix the solution in the reaction cup 100. For example, after adding the reagent to the sample, the sample and the reagent can be mixed, and after adding the substrate solution to the sample and the reagent, they can be mixed again to form a test solution with uniform distribution of the substrate solution, sample and reagent. In addition, the cells in the whole blood will naturally settle, resulting in an uneven sample, and the mixing module 4 can also make the cells in the whole blood evenly distributed. Optionally, the solution can also be mixed by the suction and exhalation action of the pipette needle 111, and / or the solution can be mixed by rotating the pipette needle.

[0166] Optionally, the mixing module 4 mixes the solution in the reaction cup 100 by moving it. For example, the mixing module 4 can directly move the solution or indirectly move the solution. For example, the mixing module 4 moves the solution by directly stirring the solution, or the mixing module 4 moves outside the reaction cup 100 to move the solution in the reaction cup 100.

[0167] Exemplarily, the mixing module 4 includes at least one of an ultrasonic mixing submodule, a vibration mixing submodule, and a rotation mixing submodule. The ultrasonic mixing submodule can generate ultrasonic waves, and the vibration mixing submodule can generate regular or irregular vibrations. The reaction cup 100 contacts the ultrasonic mixing submodule and / or the vibration mixing submodule, so that the solution in the reaction cup 100 moves, thereby making the solution evenly mixed.

[0168] The rotating mixing submodule can make the reaction cup 100 rotate, and during the rotation of the reaction cup 100, the solution moves, thereby mixing the solution. For example, the rotating mixing submodule can drive the reaction cup 100 to make a circular motion, such as a circular motion around a vertical axis. Of course, the rotating mixing submodule can drive the reaction cup 100 to move along a trajectory of other shapes, and the motion can include rotation or swinging.

[0169] like Figure 1 and Figure 5a As shown, optionally, the luminescence detection device may further include a reaction cup transfer module 6 , and the reaction cup transfer module 6 is used to transfer the reaction cup 100 .

[0170] Optionally, when the mixing module 4 includes a rotating mixing sub-module, the rotating mixing sub-module is connected to the reaction cup transfer module 6. When the solution needs to be mixed, the reaction cup transfer module 6 picks up the reaction cup 100, and the rotating mixing sub-module works to drive the reaction cup 100 to move; when the solution does not need to be mixed, but the reaction cup 100 needs to be transferred, the reaction cup transfer module 6 picks up the reaction cup 100 and drives the reaction cup 100 to move, but the rotating mixing sub-module does not work at this time.

[0171] Optionally, the cuvette transfer module 6 picks up the cuvette 100 by lifting, clamping and / or adsorption. Exemplarily, the cuvette transfer module 6 can lift the cuvette 100 from the bottom of the cuvette 100, and can put the cuvette 100 down, so as to transfer the cuvette 100 by lifting. For another example, the cuvette transfer module 6 may include two clamps, the two clamps are close to each other, and can clamp the cuvette 100, and the two clamps are far away from each other to release the cuvette 100, such as the cuvette transfer module 6 can be a clamp cylinder; for another example, the cuvette transfer module 6 adsorbs the cuvette 100 by vacuum adsorption, magnetic adsorption and other adsorption methods. When vacuum adsorption of the cuvette is adopted, a vacuum can be formed between the cuvette 100 and the cuvette transfer module 6, so as to achieve the purpose of adsorbing the cuvette 100; when magnetic adsorption is adopted, the cuvette can be adsorbed by magnet adsorption or electromagnet adsorption, such as a magnetic block connected to the cuvette 100, so as to adsorb the cuvette 100 by magnetic adsorption.

[0172] Optionally, the reaction cup transfer module 6 can transfer the reaction cup 100 in three-dimensional space, and / or the reaction cup transfer module 6 can rotate, for example, the motion trajectory of the reaction cup transfer module 6 after grabbing the reaction cup 100 is an arc trajectory or a straight line trajectory, or is composed of multiple arc trajectories and / or multiple straight line trajectories, or is composed of one arc trajectory and multiple straight line trajectories, or is composed of multiple arc trajectories and one straight line trajectory.

[0173] like Figure 2 As shown, the reaction cup transfer module 6 includes a first rotating drive member, a first rotating arm 61, a second rotating drive member, a second rotating arm 62 and a pick-up and place member 63 connected in sequence. The first rotating drive member drives the first rotating arm 61 to rotate, the second rotating drive member drives the second rotating arm 62 to rotate, and the pick-up and place member 63 can realize the above-mentioned lifting, clamping and / or adsorption functions. Figure 5a As shown, optionally, a rotating mixing submodule can be connected between the second rotating arm 62 and the pick-and-place member 63. In another optional embodiment, the reaction cup transfer module 6 can also include a first direction moving part, a second direction moving part, a third direction moving part and a pick-and-place member 63 connected in sequence, the first direction moving part can make the second direction moving part move along the first direction, the second direction moving part can make the third direction moving part move along the second direction, the third direction moving part can make the pick-and-place member 63 move along the third direction, and the first direction, the second direction and the third direction can be set at an angle to each other, so that the pick-and-place member 63 moves in a three-dimensional space. Exemplarily, the three directions are perpendicular to each other, and further, the three directions are perpendicular to each other, and one of the three directions is a vertical direction.

[0174] like Figure 1-Figure 3As shown, the luminescence detection device further includes an incubation module 8, which is used to incubate the solution in the reaction cup 100. The incubation module 8 can provide a suitable environmental temperature for the reagent to react.

[0175] like Figure 1 and Figure 4 As shown, illustratively, after the test solution in the reaction cup 100 is evenly mixed, the reaction cup 100 is placed in the incubation module 8, illustratively, as Figure 1 As shown, the incubation module 8 can accommodate a reaction cup 100, or Figure 4 As shown, the incubation module 8 accommodates a plurality of cuvettes 100. The cuvette 100 containing the test liquid can be transferred to the incubation module 8 by the cuvette transfer module 6. After the incubation is completed, the cuvette 100 of the incubation module 8 can be transferred to the detection module 3 by the cuvette transfer module 6.

[0176] Different from Figure 1 and Figure 4 ,like Figure 2 and Figure 3 As shown, illustratively, an empty reaction cup 100 can be placed on the incubation module 8, and then the test liquid can be prepared on the incubation module 8. illustratively, the incubation module 8 can hold multiple reaction cups 100.

[0177] like Figure 2 and Figure 3 As shown, the incubation module 8 includes an incubation tray 81, which is used to carry the reaction cup 100 and control the temperature of the reaction cup 100 to be constant. Optionally, the incubation tray 81 can rotate, such as rotating around its own central axis. Optionally, the incubation tray 81 is provided with a plurality of incubation positions 82 spaced along its circumference, and each incubation position 82 can be placed with a reaction cup 100.

[0178] In other optional embodiments, the rotating mixing submodule may not be connected to the reaction cup transfer module 6, but, optionally, the rotating mixing submodule may be located in the incubation module 8. Exemplarily, the rotating mixing submodule is located in the incubation tray 81, that is, the solution is mixed by adding a rotating mixing subdevice to the incubation tray 81.

[0179] The liquid adding module 1, the detection module 3, the cuvette storage module 9 and / or the cuvette recovery module 10 are arranged on the side, top or bottom of the incubation tray 81. In this embodiment, the side or one side can be one side in the horizontal direction or the upper side or the lower side.

[0180] Exemplarily, the substrate loading submodule 12, the sample loading unit 112 and the reagent loading unit 113 can be arranged on the outside of the incubation tray 81, such as, the substrate loading submodule 12, the sample loading unit 112 and the reagent loading unit 113 are arranged at intervals along the circumference of the incubation tray 81. Furthermore, along the rotation direction of the incubation tray 81, the sample loading unit 112, the reagent loading unit 113 and the substrate loading submodule 12 are arranged in sequence, so that when the incubation tray 81 rotates, the same reaction cup 100 passes through the sample loading unit 112, the reagent loading unit 113 and the substrate loading submodule 12 in sequence, so that the sample, reagent and substrate solution are added to the reaction cup 100 in sequence.

[0181] like Figure 2 As shown, the incubation tray 81 can be annular, and optionally, the detection module 3 is arranged in the incubation module 8. Specifically, the detection module 3 is arranged on the rotation track of the incubation position 82 of the incubation tray 81, and the reaction cup 100 can automatically enter the detection module 3 during the rotation of the incubation tray 81. After the detection is completed, the incubation tray 81 rotates, and the reaction cup 100 can automatically come out of the detection module 3.

[0182] Optionally, the detection module 3 may include an entrance door and an exit door. When the reaction cup 100 enters the detection module 3, the entrance door and the exit door are opened, one reaction cup 100 is transferred out of the detection module 3, and another reaction cup 100 enters the detection module 3. When detecting, the entrance door and the exit door are both closed, thereby ensuring the darkroom 31 environment of the detection module 3.

[0183] Of course, in other optional embodiments, the detection module 3 can also be arranged outside the incubation module 8, such as being arranged above, below, above the side, below the side, or on the side in the horizontal direction of the incubation module 8.

[0184] like Figure 2 As shown, the reaction cup transfer module 6 can be located in the inner ring or the upper part of the ring, and the reaction cup transfer module 6 picks up the reaction cup 100 to perform operations such as mixing.

[0185] Of course, you can also Figure 3 As shown, the incubation tray 81 is a circular structure. The detection module 3 can be arranged on one side of the incubation tray 81, such as the outside of the incubation tray 81. The reaction cup transfer module 6 can be located on the outside of the incubation tray 81, and the reaction cup transfer module 6 picks up the reaction cup 100 to perform a mixing operation, and / or the reaction cup transfer module 6 transfers the reaction cup 100 from the incubation module 8 to the detection module 3.

[0186] like Figure 2As shown, the incubation module 8 may further include an incubation tray drive submodule, which is used to drive the incubation tray 81 to rotate. The incubation tray drive submodule includes a driving member, a driving gear 831 and a driven gear 832, the driving member is connected to the driving gear 831, and is used to drive the driving gear 831 to rotate, the driven gear 832 is connected to the incubation tray 81, and meshes with the driven gear 832, and the driving member can drive the incubation tray 81 to rotate. It can be understood that the structure of the incubation tray drive submodule is not limited to this, and can also be other structures.

[0187] like Figure 2-Figure 4 ,as well as Figure 6 and Figure 7 As shown, optionally, the luminescence detection device may further include a reaction cup storage module 9 and / or a reaction cup recovery module 10. The reaction cup storage module 9 is used to store unused reaction cups 100, and the reaction cup recovery module 10 is used to recover used reaction cups 100 for subsequent unified treatment. The reaction cup storage module 9 stores unused reaction cups 100, so that the reaction cups 100 can be provided at any time. After the detection is completed, the reaction cup 100 can be transferred to the reaction cup recovery module 10, which can reduce aerosols in the environment. For example, the reaction cup 100 can be transferred to the reaction cup recovery module 10 by the reaction cup transfer module 6. Figure 2 and Figure 3 As shown, optionally, the reaction cup storage module 9 and / or the reaction cup recovery module 10 are arranged on the side, upper part or lower part of the incubation tray 81 to facilitate the transfer of the reaction cup 100.

[0188] like Figure 4 As shown, illustratively, the cuvette transfer module 6 can pick up the cuvette 100 in the cuvette storage module 9, or, Figure 6 As shown, the cuvette storage module 9 can transfer the stored cuvette 100 to a preset position, and the cuvette transfer module 6 can pick up the cuvette 100 from the preset position. After picking up the cuvette 100, the cuvette transfer module 6 can place the cuvette 100 on the incubation module 8, or place the cuvette 100 under the sample loading unit 112.

[0189] like Figure 6 As shown, the cuvette storage module 9 includes a storage submodule 91 and a cuvette arrangement submodule 92. The storage submodule 91 is used to store cuvettes 100 and provide cuvettes 100 to the cuvette arrangement submodule 92. The cuvette arrangement submodule 92 is used to arrange the cuvettes 100 in a preset arrangement pattern. The cuvette transfer module 6 can pick up the cuvettes 100 on the cuvette arrangement submodule 92, or Figure 2 and Figure 3 As shown, the reaction cup arrangement submodule 92 can directly provide the reaction cups 100 to the incubation tray 81 .

[0190] Optionally, the storage submodule 91 may include a lifting base plate 911 , and the lifting base plate 911 may be moved up and down so that the reaction cup 100 on the lifting base plate 911 is transferred to the reaction cup arrangement submodule 92 through the outlet of the storage submodule 91 .

[0191] In a specific embodiment, Figure 2 The incubation module 8 includes an annular incubation tray 81, and a plurality of incubation positions 82 are arranged along the circumference of the incubation tray 81. The detection module 3 is arranged in the incubation module 8, and the plurality of incubation positions 82 can be rotated into the incubation module 8 respectively. The reaction cup transfer module 6 is arranged in the inner ring of the annular ring. Of course, in other optional embodiments, the detection module 3 is arranged outside the incubation module 8.

[0192] As an example, the sample loading unit 112, the reagent loading unit 113 and the substrate loading submodule 12 are arranged at circumferential intervals along the incubation plate 81, and the substrate loading submodule 12 is arranged close to the detection module 3 so that the reaction cup 100 facing the substrate loading submodule 12 can be rotated into the detection module 3 as quickly as possible.

[0193] As an example, the washing module 7, the sample carrying part 51, the reaction cup storage module 9 and the reaction cup recovery module 10 are all arranged outside the incubation tray 81, wherein the washing module 7 and the sample carrying part 51 are arranged near the sample loading unit 112. The reagent tank 53 is arranged near the reagent loading unit 113, and the substrate liquid tank 54 is arranged near the substrate loading submodule 12. The reaction cup storage module 9 is arranged near the sample loading unit 112 and is located upstream of the sample loading unit 112, and the reaction cup recovery module 10 is arranged near the detection module 3.

[0194] The luminescence detection device provided in this embodiment has a reasonable structure and is used for homogeneous chemiluminescence detection, and can simultaneously meet the requirements of high sensitivity, wide linear range, good repeatability, short detection time, high detection throughput, and small instrument size, and can well meet the needs of current clinical detection.

[0195] Exemplarily, a specific working process of the luminescence detection device may be as follows:

[0196] The cuvette storage module 9 transports the cuvette 100 to a preset position of the incubation tray 81; or the cuvette transfer module 6 transfers the cuvette 100 of the cuvette storage module 9 to a preset position of the incubation tray 81;

[0197] The sample loading unit 112 absorbs the sample on the sample carrying portion 51;

[0198] The incubation plate 81 rotates, the reaction cup 100 faces the sample loading unit 112, and the sample loading unit 112 adds the sample into the reaction cup 100;

[0199] The incubation plate 81 rotates, the reaction cup 100 faces the reagent loading unit 113, and the reagent loading unit 113 adds reagent into the reaction cup 100;

[0200] The reaction cup transfer module 6 picks up the reaction cup 100, and the mixing module 4 mixes the solution in the reaction cup 100;

[0201] The incubation plate 81 rotates, and when the reaction cup 100 is located in the substrate loading submodule 12, the substrate loading submodule 12 adds substrate liquid into the reaction cup 100;

[0202] The reaction cup transfer module 6 picks up the reaction cup 100, and the mixing module 4 mixes the solution in the reaction cup 100;

[0203] The incubation plate 81 rotates, the reaction cup 100 enters the dark room 31, and the detection submodule 32 performs detection;

[0204] The incubation tray 81 rotates, the cuvette 100 is located outside the darkroom 31 , the cuvette transfer module 6 picks up the cuvette 100 , and transfers the cuvette 100 to the cuvette recovery module 10 .

[0205] It can be understood that when a reaction cup 100 is facing the sample loading unit 112, one of the multiple reaction cups 100 on the incubation module 8 can be facing the reagent loading unit 113, another reaction cup 100 can be facing the substrate loading submodule 12, another reaction cup 100 can be located in the dark chamber 31, and another reaction cup 100 is transferred to the reaction cup recovery module 10.

[0206] Optionally, the multi-detection index analysis system may further include a control mechanism, which is used to control each module to work under a predetermined program. In this embodiment, the control mechanism may be a centralized or distributed controller, for example, the controller may be a single microcontroller, or may be composed of multiple distributed microcontrollers, in which a control program may be run to control the above components to realize their functions.

[0207] Embodiment 2

[0208] like Figure 3 As shown, this embodiment also provides a multi-detection index analysis system, including a biochemical analysis module 20 and the luminescence detection device provided in Embodiment 1. When the luminescence detection device includes at least one of a liquid adding module 1, a liquid carrying module 5, an incubation module 8, and a washing module 7, the biochemical analysis module 20 can be matched with at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, and the washing module 7 of the luminescence detection device.

[0209] It can be understood that the biochemical analysis module 20 matches at least one of the liquid adding module 1, liquid carrying module 5, incubation module 8 and washing module 7 of the luminescent detection device, which means that the liquid adding module 1 provides the liquid required for detection for the biochemical analysis module 20 and the detection module 3 at the same time, and the liquid carried by the liquid carrying module 5 is the liquid required by the biochemical analysis module 20 and the detection module 3, wherein the liquid can be at least one of a reagent, a sample and a substrate liquid. The incubation module 8 can incubate the test liquid (a mixture of reagents, samples and substrate liquids) detected by the biochemical analysis module 20 and the detection module 3. The washing module 7 can wash the liquid adding module 1 and / or the stirring module 40 after the addition of liquid.

[0210] like Figure 3 As shown, illustratively, the biochemical analysis module 20 matches the liquid adding module 1, the liquid carrying module 5, the incubation module 8 and the washing module 7 of the luminescence detection device.

[0211] The incubation module 8 includes an outer incubation tray 811 and an inner incubation tray 812, wherein the outer incubation tray 811 is an annular structure, and the inner incubation tray 812 is arranged in the outer incubation tray 811, and the inner incubation tray 812 can be circular or annular. The reaction cup 100 carried by the outer incubation tray 811 contains a solution to be analyzed by biochemical analysis and a solution to be detected by homogeneous luminescence, and the reaction cup 100 carried by the outer incubation tray 811 contains another solution to be analyzed by biochemical analysis and a solution to be detected by homogeneous luminescence. Preferably, the shape and / or size of the reaction cup 100 containing the solution for biochemical analysis and the reaction cup 100 containing the solution for luminescence detection can be different, and they are reaction cups 100 with different appearances. Of course, the two reaction cups 100 can also be reaction cups with the same appearance. For ease of explanation, in this embodiment, the reaction cup 100 containing the solution for biochemical analysis is named as biochemical reaction cup 101, and the reaction cup 100 containing the solution for luminescence detection is named as luminescence reaction cup 102.

[0212] Specifically, the outer incubation tray 811 and the inner incubation tray 812 can be coaxially arranged, and the outer incubation tray 811 and / or the inner incubation tray 812 can rotate, such as the outer incubation tray 811 and the inner incubation tray 812 can rotate together, or can rotate independently. The outer incubation tray 811 can be provided with a plurality of biochemical reaction cups 101 at intervals along its circumference, and the inner incubation tray 812 can be provided with a plurality of luminous reaction cups 102 at intervals along its circumference.

[0213] In other optional embodiments, the incubation module 8 may also include an incubation tray 81, on which two circles of incubation positions 82 may be provided, and each circle may be used to place the required reaction cups 100 as needed, such as placing the biochemical reaction cup 101 in the outer circle and the luminous reaction cup 102 in the inner circle (of course, the luminous reaction cup 102 may also be placed in the outer circle and the biochemical reaction cup 101 may be placed in the inner circle).

[0214] A stirring module 40 may also be provided on the outside of the incubation tray 81, and the stirring module 40 may extend into the reaction cup 100 to stir and mix the solution. Of course, the stirring module 40 may also be located on the inside of the incubation tray 81, wherein the inside and the outside may be one side in the horizontal direction, or may be the upper side or the lower side. The stirring module 40 may also be located directly above the incubation tray 81.

[0215] Exemplarily, a liquid carrying module 5 may be further provided on one side, above or below the incubation tray 81, wherein the one side may be a side in the horizontal direction, or may be above or below the side. Exemplarily, the liquid carrying module 5 includes a sample carrying portion 51, a substrate liquid tank 54 and a reagent tank 53, the sample carrying portion 51 is annular, the substrate liquid tank 54 and the reagent tank 53 are both provided in the annular portion of the sample carrying portion 51, of course, the positions of the substrate liquid tank 54 and the reagent tank 53 are not limited thereto, the substrate liquid tank 54 and the reagent tank 53 may also be provided outside the annular portion of the sample carrying portion 51.

[0216] Exemplarily, the liquid adding module 1 includes a reagent loading unit 113, a sample loading unit 112 and a substrate loading submodule 12, wherein the reagent loading unit 113, the sample loading unit 112, the washing module 7 and the detection module 3 are arranged between the incubation tray 81 and the liquid carrying module 5. The washing module 7 is located on one side, above or below the reagent loading unit 113. The substrate loading submodule 12 is located between the detection module 3 and the liquid carrying module 5. Of course, the above layout can be adjusted as needed.

[0217] The reaction cup storage module 9 and the biochemical analysis module 20 are arranged on one side, above or below the incubation tray 81. The multi-detection index analysis system may further include a biochemical reaction cup cleaning module 50, which is used to clean the biochemical reaction cup 101. The biochemical reaction cup cleaning module 50 may be arranged on one side, above or below the biochemical analysis module 20 to facilitate the cleaning of the biochemical reaction cup 101.

[0218] Optionally, the cuvette transfer module 6 is disposed between the cuvette storage module 9 and the detection module 3 .

[0219] Exemplarily, a specific working process of the multi-detection index analysis system can be as follows:

[0220] The reaction cup transfer module 6 transfers the luminescent reaction cup 102 of the reaction cup storage module 9 to the inner incubation tray 812; the biochemical reaction cup 101 is manually placed on the outer incubation tray 811;

[0221] The sample loading unit 112 absorbs the sample on the sample carrying portion 51;

[0222] The incubation plate 81 rotates, and the biochemical reaction cup 101 and / or the luminescent reaction cup 102 can be directly opposite to the sample loading unit 112, and the sample loading unit 112 adds the sample into the reaction cup 101;

[0223] The incubation tray 81 rotates, and the biochemical reaction cup 101 and / or the luminescent reaction cup 102 can be directly opposite to the reagent loading unit 113, and the reagent loading unit 113 adds reagents into the biochemical reaction cup 101 and / or the luminescent reaction cup 102;

[0224] The reaction cup transfer module 6 picks up the biochemical reaction cup 101 and / or the luminescent reaction cup 102, the mixing module 4 mixes the solution in the luminescent reaction cup 102; the stirring module 40 stirs the solution in the biochemical reaction cup 101;

[0225] The incubation tray 81 rotates, and when the biochemical reaction cup 101 and / or the luminescent reaction cup 102 is located in the substrate loading submodule 12, the substrate loading submodule 12 adds substrate liquid into the biochemical reaction cup 101 and / or the luminescent reaction cup 102;

[0226] The reaction cup transfer module 6 picks up the biochemical reaction cup 101 and / or the luminescent reaction cup 102, the mixing module 4 mixes the solution in the luminescent reaction cup 102; the stirring module 40 stirs the solution in the biochemical reaction cup 101;

[0227] The reaction cup transfer module 6 transfers the luminescent reaction cup 102 to the dark room 31, and the detection submodule 32 performs detection; and / or the incubation plate 81 rotates, and the biochemical reaction cup 101 can face the biochemical analysis module 20, and the biochemical analysis module 20 analyzes the solution;

[0228] The incubation tray 81 rotates, the luminescent reaction cup 102 is located outside the dark chamber 31 , the reaction cup transfer module 6 picks up the luminescent reaction cup 102 , and transfers the luminescent reaction cup 102 to the reaction cup recovery module 10 .

[0229] It can be understood that when a reaction cup 100 is facing the sample loading unit 112, one of the multiple reaction cups 100 on the incubation module 8 can be facing the reagent loading module, another reaction cup 100 can be facing the substrate loading submodule 12, and another reaction cup 100 can be located in the darkroom 31, one of the reaction cups 100 is facing the biochemical analysis module 20, and another reaction cup 100 is transferred to the reaction cup recovery module 10.

[0230] Optionally, the biochemical analysis module 20 may be connected to a control mechanism, which is used to control each module to work under a predetermined program, such as the control mechanism may control at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8 and the washing module 7 to be matched and used with the biochemical analysis module 20 and the detection module 3, respectively. In this embodiment, the control mechanism may be a centralized or distributed controller, for example, the controller may be a single microcontroller or may be composed of multiple distributed microcontrollers, and a control program may be run in the microcontroller to control the above components to realize their functions.

[0231] Embodiment 3

[0232] like Figure 4 As shown, this embodiment also provides a multi-detection index analysis system including a blood coagulation analysis module 30 and the luminescence detection device provided in the first embodiment.

[0233] Exemplarily, the blood coagulation analysis module 30 can be used to detect prothrombin time (PT), activated partial thromboplastin time (APTT), thrombin time (TT), fibrinogen (FIB), coagulation factors II, V, VII, X, VIII, IX, XI, XII, antithrombin III (AT-III), protein C (PC), protein S (PS), anti-activated protein C (APCR), lupus anticoagulant (LAC), plasminogen (PLG), α2-antiplasmin (α2-AP), FDP and / or D-dimer (D-Dimer) and other items. Exemplarily, the blood coagulation analysis module 30 can be used for detection by coagulation method, substrate colorimetric method, immunoassay, latex agglutination method and other methods. Among them, the coagulation method can be divided into two categories: optical method and magnetic bead method.

[0234] When the luminescence detection device includes at least one of a liquid adding module 1, a liquid carrying module 5, an incubation module 8, a mixing module 4, a recovery module, a reaction cup storage module 9 and a washing module 7, the blood coagulation analysis module 30 matches and uses at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, the recovery module, the reaction cup storage module 9, the mixing module 4 and the washing module 7 of the luminescence detection device.

[0235] It can be understood that the blood coagulation analysis module 30 matches at least one of the liquid adding module 1, liquid carrying module 5, incubation module 8 and washing module 7 of the luminescent detection device, which means that the liquid adding module 1 provides the blood coagulation analysis module 30 and the detection module 3 with the liquid required for detection at the same time, and the liquid carried by the liquid carrying module 5 is the liquid required by the blood coagulation analysis module 30 and the detection module 3, wherein the liquid can be at least one of a reagent, a sample and a substrate liquid. The incubation module 8 can incubate the mixture (a mixture of reagents, samples and substrate liquid) that needs to be detected by the blood coagulation analysis module 30 and the detection module 3. The washing module 7 can wash the liquid adding module 1 after adding liquid. The mixing module 4 can mix the solution required for blood coagulation analysis, and can also mix the solution required for homogeneous luminescent detection. The recovery module can recover the reaction cup 100 at the blood coagulation analysis module 30, and can also recover the reaction cup 100 at the detection module 3; the reaction cup storage module 9 can store the reaction cup 100 required at the blood coagulation analysis module 30, and can also store the reaction cup 100 required by the detection module 3.

[0236] In a specific embodiment, the incubation module 8 accommodates a plurality of reaction cups 100, and at least the darkroom 31 of the detection module 3 is disposed in the incubation module 8. A sample adding position 70 and / or a reagent adding position 60 are disposed on the outside of the incubation module 8, a reagent compartment 53 and a reagent loading unit 113 may be disposed on one side of the reagent adding position 60, a sample loading unit 112 may be disposed on one side of the sample adding position 70, and a sample carrying portion 51 may be disposed on one side of the sample loading unit 112. A washing module 7 may be disposed between the reagent loading unit 113 and the sample loading unit 112. A reaction cup storage module 9 may be disposed on the outside of the incubation module 8, and a reaction cup transfer module 6 may be disposed between the incubation module 8, the reaction cup storage module 9 and the sample adding position 70. A blood coagulation analysis module 30 may be disposed on one side outside the incubation module 8.

[0237] Exemplarily, a specific working process of the multi-detection index analysis system can be as follows:

[0238] The cuvette transfer module 6 picks up the empty cuvette 100 on the cuvette storage module 9 and places the cuvette 100 on the sample adding position 70;

[0239] The sample loading unit 112 absorbs the reagent in the reagent carrying part, the sample loading unit 112 adds the sample into the sample cup, and the washing module 7 washes the sample loading unit 112;

[0240] The cuvette transfer module 6 picks up the cuvette 100 on the sample adding position 70 and transfers the cuvette 100 to the reagent adding position 60;

[0241] The reagent loading unit 113 absorbs the reagent in the reagent compartment 53 and adds the reagent into the sample cup;

[0242] The cuvette transfer module 6 transfers the cuvette 100 on the reagent adding position 60 to the incubation module 8;

[0243] After a certain incubation period, the substrate loading submodule 12 adds the substrate solution into the reaction cup 100 of the incubation module 8 ; and / or the reagent loading unit 113 draws the coagulation reagent from the reagent compartment 53 and adds it into the reaction cup 100 of the incubation module 8 .

[0244] The incubation module 8 drives the reaction cup 100 to enter the detection module 3 or the blood coagulation analysis module 30 for detection and analysis; or the reaction cup transfer module 6 transfers the reaction cup 100 to the detection module 3 or the blood coagulation analysis module 30 for detection and analysis;

[0245] After the detection and analysis are completed, the cuvette transfer module 6 transfers the cuvette 100 at the detection module 3 or the blood coagulation analysis module 30 to the cuvette recovery module 10 .

[0246] Optionally, after adding the reagent and / or substrate solution, the solution may be mixed.

[0247] Optionally, the sample loading unit 112 and the reagent loading unit 113 may be used in combination.

[0248] Optionally, the blood coagulation analysis module 30 may be connected to a control mechanism, and the control mechanism is used to control each module to work under a predetermined program, for example, the control mechanism may control at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, the mixing module 4, the recovery module, the reaction cup storage module 9 and the washing module 7 to be matched and used with the blood coagulation analysis module 30 and the detection module 3 respectively. In this embodiment, the control mechanism may be a centralized or distributed controller, for example, the controller may be a single microcontroller, or may be composed of multiple distributed microcontrollers, and the control program may be run in the microcontroller to control the above components to realize their functions.

[0249] Embodiment 4

[0250] like Figure 3 As shown, this embodiment also provides a multi-detection index analysis system including a blood cell analysis module and the luminescence detection device provided in the first embodiment.

[0251] When the luminescence detection device includes at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, the mixing module 4 and the washing module 7, the blood cell analysis module matches and uses at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, the mixing module 4 and the washing module 7 of the luminescence detection device;

[0252] like Figure 5bAs shown, optionally, another mixing device may be included to mix the sample in the sample tube 52 so that the blood cells in the sample tube 52 are evenly distributed.

[0253] It can be understood that the blood cell analysis module matches at least one of the liquid adding module 1, liquid carrying module 5, incubation module 8 and washing module 7 of the luminescent detection device, which means that the liquid adding module 1 provides the liquid required for detection for the blood cell analysis module and the detection module 3 at the same time, and the liquid carried by the liquid carrying module 5 is the liquid required by the blood cell analysis module and the detection module 3, wherein the liquid can be at least one of a reagent, a sample and a substrate liquid. The incubation module 8 can incubate a mixture (a mixture of reagents, samples and substrate liquid) that needs to be detected by the blood cell analysis module and the detection module 3. The washing module 7 can wash the liquid adding module 1 after liquid addition. The mixing module 4 can mix the solution required for blood cell analysis, and can also mix the solution required for homogeneous luminescent detection.

[0254] When the multi-detection index analysis system includes a blood cell analysis module and a luminescence detection device, the existing blood cell analyzer can be used. When the blood cell analyzer and the luminescence detection device both have any one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, the mixing module 4 and the washing module 7, one of the blood cell analyzer and the luminescence detection device can omit the above-mentioned modules that match the blood cell analyzer, thereby simplifying the structure of the multi-detection index analysis system.

[0255] Optionally, the blood cell analysis module may be connected to a control mechanism, and the control mechanism is used to control each module to work under a predetermined program, for example, the control mechanism may control at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8, the mixing module 4 and the washing module 7 to be matched and used with the blood cell analysis module and the detection module 3 respectively. In this embodiment, the control mechanism may be a centralized or distributed controller, for example, the controller may be a single microcontroller, or may be composed of multiple distributed microcontrollers, and the control program may be run in the microcontroller to control the above components to realize their functions.

[0256] Exemplarily, after collecting and mixing the samples, a portion of the samples is allocated to the blood cell analysis module for routine blood testing, and the other portion is allocated to the luminescence detection device. Exemplarily, the samples allocated to the luminescence detection device are tested in the following steps: 1) Take the reaction cup 100 and add the sample to the reaction cup 100; 2) Add a reagent (for example, the reagent can be a luminescent reagent) to the reaction cup 100, mix the reagent and the sample, and incubate the sample and the reagent at a specific temperature for a certain period of time; 3) Add a substrate solution to the reaction cup 100, mix the sample, the reagent and the sample, and detect the luminescence signal; 4) Calculate the content of the analyte based on the luminescence signal and the routine blood data.

[0257] Embodiment 5

[0258] This embodiment further provides a multi-detection index analysis system, comprising at least two of a biochemical analysis module 20, a blood cell analysis module and a blood coagulation analysis module 30, and the luminescence detection device provided in the first embodiment.

[0259] Optionally, the biochemical analysis module 20 may be the biochemical analysis module 20 in the second embodiment, the blood coagulation analysis module 30 may be the blood coagulation analysis module 30 in the third embodiment, and the blood cell analysis module may be the blood cell analysis module in the fourth embodiment.

[0260] At least two of the biochemical analysis module 20, the blood cell analysis module and the blood coagulation analysis module 30 (modules included in the multi-detection index analysis system) can be connected to the control mechanism, and the control mechanism is used to control each module to work under a predetermined program. For example, the control mechanism can control some modules, such as at least one of the liquid adding module 1, the liquid carrying module 5, the incubation module 8 and the washing module 7, etc., to be matched and used with at least two of the biochemical analysis module 20, the blood cell analysis module and the blood coagulation analysis module 30 and the detection module 3. In this embodiment, the control mechanism can be a centralized or distributed controller. For example, the controller can be a single microcontroller or a distributed multi-microcontroller. The control program can be run in the microcontroller to control the above components to realize their functions.

[0261] Embodiment 6

[0262] like Fig. 9 As shown, this embodiment provides a detection method, wherein the luminescence detection device provided in the first embodiment can execute the detection method.

[0263] Detection methods include:

[0264] Detection: The reaction cup 100 is placed at the detection module 3, and the detection module 3 detects the liquid to be tested in the reaction cup 100. It can be understood that the liquid to be tested is a mixture of a sample, a reagent and a substrate liquid.

[0265] Prior to testing, you may also include:

[0266] Providing the reaction cup 100: the reaction cup transfer module 6 picks up the reaction cup 100 provided by the reaction cup storage module 9 or the reaction cup storage module 9 provides the reaction cup 100 to the incubation module 8; at this time, the reaction cup 100 is an empty reaction cup 100, and this step can realize the automatic provision of the reaction cup 100 to the incubation module 8.

[0267] Before testing, it also includes:

[0268] Preparation of the liquid to be tested: the liquid adding module 1 adds the liquid required for the test into the reaction cup 100 , so that the liquid forms the liquid to be tested in the reaction cup 100 .

[0269] Optionally, the step of preparing the test liquid may be performed after providing the reaction cup 100 .

[0270] Specifically, preparing the test solution includes:

[0271] Adding sample: the liquid adding module 1 adds the sample to the reaction cup 100; and / or,

[0272] Adding reagents: the liquid adding module 1 adds reagents to the reaction cup 100; and / or,

[0273] Adding substrate liquid: the liquid adding module 1 adds the substrate liquid into the reaction cup 100 .

[0274] In a specific embodiment, preparing the test solution includes sequentially adding a sample, a reagent, and a substrate solution into the reaction cup 100. Optionally, the sample may be added first, then the reagent, and finally the substrate solution. In other optional embodiments, the reagent may be added first, then the sample, and finally the substrate solution.

[0275] Before adding samples, sampling can also be included, and sampling includes:

[0276] The liquid adding module 1 moves to the liquid carrying module 5, such as the sample loading unit 112 moves to the sample loading portion 51; and / or the liquid carrying module 5 moves to the liquid adding module 1, such as the sample loading portion 51 moves to the sample loading unit 112;

[0277] The liquid adding module 1 absorbs the sample in the liquid carrying module 5 , for example, the sample loading unit 112 absorbs the reagent in the sample carrying portion 51 .

[0278] Optionally, adding samples may include:

[0279] like Figure 1 and Figure 4 As shown, the reaction cup transfer module 6 transfers the reaction cup 100 to the bottom of the liquid adding module 1; or the liquid adding module 1 moves to the top of the reaction cup 100; or, as shown Figure 2 and Figure 3 As shown, the incubation module 8 transfers the reaction cup 100 to the bottom of the liquid adding module 1, specifically, the incubation plate 81 of the incubation module 8 rotates, so that the reaction cup 100 is transferred to the sample loading unit 112. That is, when adding the sample, the reaction cup 100 is vertically opposite to the liquid adding module 1, specifically, the reaction cup 100 is opposite to the sample loading unit 112;

[0280] The liquid adding module 1 adds the sample into the reaction cup 100 .

[0281] Preferably, after adding the sample, the following steps may also be included:

[0282] Cleaning the liquid adding module 1: The washing module 7 cleans the liquid adding module 1. Specifically, the washing module 7 cleans the sample loading unit 112, thereby avoiding cross contamination between samples.

[0283] Optionally, before adding the reagent, the step of taking the reagent includes:

[0284] The liquid adding module 1 moves to the liquid carrying module 5, such as the reagent loading unit 113 moves to the reagent compartment 53; and / or the liquid carrying module 5 moves to the liquid adding module 1, such as the reagent compartment 53 moves to the reagent loading unit 113;

[0285] The liquid adding module 1 absorbs the reagent in the liquid carrying module 5 , for example, the reagent loading unit 113 absorbs the reagent in the reagent chamber 53 .

[0286] In other optional embodiments, the reagent loading unit 113 may be in communication with the reagent chamber 53 , thereby eliminating the steps of moving the liquid adding module 1 to the liquid carrying module 5 , and / or moving the liquid carrying module 5 to the liquid adding module 1 .

[0287] Add reagents include:

[0288] like Figure 1 and Figure 4 As shown, the reaction cup transfer module 6 transfers the reaction cup 100 to the bottom of the liquid adding module 1; or the liquid adding module 1 moves to the top of the reaction cup 100; or, as shown Figure 2 and Figure 3 As shown, the incubation module 8 transfers the reaction cup 100 to the bottom of the liquid adding module 1, specifically, the incubation tray 81 of the incubation module 8 rotates, so that the reaction cup 100 is transferred to the reagent loading unit 113. That is, when adding reagents, the reaction cup 100 is vertically opposite to the liquid adding module 1, specifically, the reaction cup 100 is opposite to the reagent loading unit 113;

[0289] The liquid adding module 1 adds the reagent to the reaction cup 100 . Specifically, the reagent loading unit 113 adds the reagent to the reaction cup 100 .

[0290] Optionally, adding the substrate solution comprises:

[0291] like Figure 1 and Figure 2 As shown, the reaction cup transfer module 6 transfers the reaction cup 100 to the bottom of the liquid adding module 1; or the liquid adding module 1 moves to the top of the reaction cup 100; or, as shown Figure 2 and Figure 3As shown, the incubation module 8 transfers the reaction cup 100 to the bottom of the liquid adding module 1, specifically, the incubation tray 81 of the incubation module 8 rotates, so that the reaction cup 100 is transferred to the substrate loading submodule 12. That is, when adding the substrate liquid, the reaction cup 100 is vertically opposite to the liquid adding module 1, specifically, the reaction cup 100 is opposite to the substrate loading submodule 12.

[0292] The liquid adding module 1 adds the substrate liquid into the reaction cup 100 . Specifically, the substrate loading submodule 12 loads the substrate liquid into the reaction cup 100 .

[0293] Optionally, after adding the reagent and / or after adding the substrate solution, the method further comprises:

[0294] Cleaning: The washing module 7 cleans the liquid adding module 1 and / or the stirring module 40. For example, after the liquid adding module 1 is loaded with samples and / or reagents, the washing module 7 cleans the liquid adding module 1, specifically, the sample loading unit 112 and / or the cleaning substrate loading submodule 12 and / or the cleaning reagent loading unit 113 and / or the stirring module 40. Exemplarily, the stirring module 40 is cleaned each time the stirring module 40 is used. The sample loading unit 112 is cleaned each time the sample is loaded.

[0295] Optionally, after adding the reagent and / or after adding the substrate solution, the method further comprises:

[0296] Mixing: The mixing module 4 mixes the liquid in the reaction cup 100.

[0297] Before testing, it also includes:

[0298] Incubation: The reaction cup 100 is incubated at a preset temperature of the incubation module 8 for a preset time, such as 3-5 minutes.

[0299] like Figure 1 As shown, optionally, the incubation step is performed after adding reagents, samples and substrate solutions; or, as Figure 2 As shown, the incubation is performed after the sample addition step; or as shown Figure 4 As shown, incubation is performed after the reagent adding step. In a specific embodiment, the sample and the reagent are added to the reaction cup 100 and then incubated. Furthermore, after the sample and the reagent are added to the reaction cup 100, they are mixed and then incubated.

[0300] Prior to testing, you may also include:

[0301] Transfer reaction cup 100: Figure 1 As shown, the cuvette transfer module 6 transfers the cuvette 100 to the detection module 3; or Figure 2 and Figure 3 As shown, the incubation module 8 transfers the reaction cup 100 to the detection module 3 .

[0302] Furthermore, the step of transferring the cuvette 100 is performed after the incubation step.

[0303] Optionally, the detection may also include:

[0304] Recovering the cuvette 100 : The cuvette transfer module 6 transfers the cuvette 100 to the cuvette recovery module 10 .

[0305] Illustratively, the detection method provided in this embodiment controls each module to perform corresponding actions through a program in a control mechanism.

[0306] The detection method provided in this embodiment is simple, the steps are reasonable, and the detection efficiency and detection accuracy can be improved.

[0307] Embodiment 7

[0308] In this embodiment, various modules of the luminescence detection device are introduced according to the circulation order of the reaction cup 100 .

[0309] like Figure 10-13 As shown, the luminescence detection device of this embodiment includes: a liquid adding module 1, a detection module 3, a mixing module 4, a liquid carrying module 5, a reaction cup transfer module 6, a washing module 7, an incubation module 8, a reaction cup storage module 9, a reaction cup recovery module 10 and a reaction cup temporary storage module 80.

[0310] The detection module 3 is used to detect the optical signal in the reaction cup 100. The incubation module 8 is used to incubate the solution in the reaction cup 100. The reaction cup transfer module 6 is used to transfer the reaction cup 100. The liquid adding module 1 is used to add the liquid required for detection to the reaction cup 100. The liquid carrying module 5 is used to carry at least one of the substrate liquid, the sample and the reagent. The mixing module 4 is used to mix the solution in the reaction cup 100. The reaction cup storage module 9 is used to store unused reaction cups 100. The reaction cup temporary storage module 80 is used to temporarily store the reaction cups 100 taken out from the reaction cup storage module 9. The reaction cup recovery module 10 is used to recover the detected reaction cups 100.

[0311] like Fig.12 As shown, the cuvette storage module 9 includes a storage submodule 91 and a cuvette arrangement submodule 92. The storage submodule 91 is used to store unused cuvettes 100 and provide cuvettes 100 to the cuvette arrangement submodule 92. The cuvette arrangement submodule 92 is used to arrange the cuvettes 100 according to a preset arrangement rule.

[0312] like Fig.12As shown, the cuvette arrangement submodule 92 includes an inclined arrangement channel 921, the upper end of which is connected to the storage submodule 91, and is used to receive the cuvettes 100 output from the storage submodule 91. When the cuvette 100 moves to the upper end of the arrangement channel 921, the cuvette 100 enters the arrangement channel 921 along the axial direction with the bottom inclined downward and the top inclined upward, and moves downward along the extension direction of the arrangement channel 921, and finally falls out from the lower end of the arrangement channel 921.

[0313] like Fig.12 As shown, the luminescence detection device further includes a reaction cup temporary storage module 80, which is arranged at the exit of the reaction cup arrangement submodule 92. Fig.12 As shown, the cuvette temporary storage module 80 is arranged at the lower end (ie, the outlet) of the arrangement channel 921 to receive the cuvette 100 dropped from the arrangement channel 921. The cuvette temporary storage module 80 is arranged to temporarily store the cuvette, which is convenient for the cuvette transfer module 6 to clamp the cuvette.

[0314] like Fig.12 As shown, the reaction cup temporary storage module 80 includes a reaction cup temporary storage seat 801 arranged at the lower end of the arrangement channel 921, and a reaction cup temporary storage groove is arranged on the reaction cup temporary storage seat 801. The reaction cup temporary storage groove is used to receive the reaction cup 100 dropped from the arrangement channel 921. When the reaction cup 100 enters the reaction cup temporary storage groove, the top of the reaction cup 100 faces upward and the bottom faces downward, and it basically remains upright.

[0315] It should be noted that the number of reaction cup temporary storage slots can be one or more. Fig.12 In the embodiment, the number of temporary storage slots for reaction cups is 4.

[0316] like Fig.13 As shown, the cuvette temporary storage module 80 further includes a first motor 802, which is used to drive the cuvette temporary storage seat 801 to rotate, thereby adjusting the position of the cuvette temporary storage slots, so as to align different cuvette temporary storage slots with the outlets of the arrangement channel 921, so that the cuvettes 100 in the arrangement channel 921 can enter different cuvette temporary storage slots. The first motor 802 is also used to rotate the cuvette 100 to a position suitable for the cuvette transfer module 6 to clamp.

[0317] like Fig.13 As shown, when there are multiple reaction cup temporary storage slots, the multiple reaction cup temporary storage slots can be arranged in a circumferential direction, and the lower end of the arrangement channel 921 is located on the rotation trajectory formed by the rotation of the reaction cup temporary storage slots, so that each reaction cup temporary storage slot can be aligned with the outlet of the arrangement channel 921.

[0318] like Fig.13As shown, the luminescence detection device also includes a reaction cup transfer module 6, which is used to transfer the reaction cup 100 from the reaction cup temporary storage module 80 to the incubation module 8. The reaction cup transfer module 6 is also used to transfer the reaction cup 100 between the incubation module 8 and the mixing module 4. The reaction cup transfer module 6 is also used to transfer the detected reaction cup 100 to the reaction cup recovery module 10.

[0319] like Fig.13 As shown, the cuvette transfer module 6 is used to transfer the cuvette 100 in the cuvette temporary storage seat 801 to the outside of the cuvette temporary storage seat 801. The cuvette transfer module 6 includes a first moving assembly 64, a second moving assembly 65 and a cuvette clamping assembly 66. The execution end of the first moving assembly 64 can move along a first direction, and the second moving assembly 65 is connected to the execution end of the first moving assembly 64 so that the second moving assembly 65 can move along the first direction. The execution end of the second moving assembly 65 can move along a second direction different from the first direction, and the cuvette clamping assembly 66 is connected to the execution end of the second moving assembly 65 so that the cuvette clamping assembly 66 can move along the second direction. Under the mutual cooperation of the first moving assembly 64 and the second moving assembly 65, the cuvette clamping assembly 66 can move along the first direction and / or the second direction.

[0320] It should be noted that the first direction and the second direction are arranged at an angle, for example, the first direction and the second direction are perpendicular to each other. As an example, Fig.13 In the embodiment, the first direction may be a horizontal direction and the second direction may be a vertical direction.

[0321] The cuvette clamping assembly 66 includes a pair of clamping jaws (i.e., pick-up and place components), and the opening and closing movements of the pair of clamping jaws can realize the gripping and releasing of the cuvette 100. The clamping jaws are preferably cylinder-driven grippers.

[0322] The moving track of the cuvette clamping assembly 66 can at least cover one of the cuvette temporary storage slots, so that the cuvette clamping assembly 66 can clamp the cuvette 100 in the cuvette temporary storage slot.

[0323] It should be noted that the specific structures of the first moving assembly 64 , the second moving assembly 65 and the reaction cup clamping assembly 66 can refer to the structures known in the prior art, and will not be described in detail here.

[0324] like Figure 10-13 As shown, the luminescence detection device also includes an incubation module 8, which is on the moving track of the reaction cup transfer module 6. That is, as shown in the figure, the incubation module 8 is located on the moving track of the reaction cup clamping assembly 66, so that the reaction cup clamping assembly 66 can transfer the reaction cup 100 in the reaction cup temporary storage tank to the incubation module 8.

[0325] like Fig.14 As shown, the incubation module 8 includes a rotatable incubation tray 81, on which a plurality of incubation positions 82 arranged in a circumferential direction are provided. By rotating the incubation tray 81, at least one incubation position 82 can be located on the moving track of the reaction cup clamping assembly 66, so that the reaction cup clamping assembly 66 can place the reaction cup 100 in the incubation position 82.

[0326] like Fig.11 and Fig.12 As shown, the luminescence detection device also includes a reagent chamber 53 and a reagent loading unit 113, which are arranged on one side of the incubation tray 81. The reagent chamber 53 is used to store reagents, and the reagent loading unit 113 is used to add the reagents in the reagent chamber 53 to the reaction cup 100 located in the incubation tray 81, for example, to the reaction cup 100 at the reagent adding station of the incubation module 8.

[0327] like Fig.14 As shown, the reagent compartment 53 includes a reagent table 531 , on which a reagent placement portion 532 is disposed, and a reagent bottle for containing a reagent can be placed on the reagent placement portion 532 .

[0328] The number of reagent placement sections 532 may be one or more. For example, when luminescence detection requires a variety of different reagents, a plurality of reagent placement sections 532 may be provided accordingly. Fig.14 There are 8 reagent storage areas.

[0329] When there are multiple reagent placement sections 532 , the reagent placement sections 532 can be arranged in a preset shape, for example, multiple reagent placement sections 532 are arranged along a circumferential direction, or linearly arranged, etc.

[0330] like Fig.12 As shown, the reagent loading unit 113 includes a reagent pipetting needle 1111, a first lifting assembly 1131 for driving the reagent pipetting needle 1111 to rise and fall, and a rotating assembly 1132 for driving the reagent pipetting needle 1111 to rotate. Through the rotating assembly 1132 and the first lifting assembly 1131, the reagent pipetting needle 1111 can move back and forth between the reagent placement portion 532 and the incubation tray 81, so as to add the reagent located in the reagent placement portion 532 to the reaction cup 100 located in the incubation tray 81. In this embodiment, the first lifting assembly 1131 preferably adopts a telescopic cylinder. The rotating assembly 1132 is preferably driven by a stepping motor, and the stepping motor drives the reagent pipetting needle 1111 to rotate through a belt drive, so that the distance can be accurately controlled.

[0331] like Fig.14As shown, the luminescence detection device also includes a washing module 7, and the washing module 7 includes a reagent washing submodule 71, and the reagent washing submodule 71 is used to wash the reagent loading unit 113. Specifically, the reagent washing submodule 71 is used to wash the reagent pipetting needle 1111. After each use of the reagent loading unit 113, the reagent pipetting needle 1111 is cleaned to avoid mutual contamination of reagents and ensure the accurate amount of reagents loaded in the experiment.

[0332] exist Fig.14 In the embodiment, the reagent washing submodule 71 is located between the reagent compartment 53 and the incubation tray 81 and is located on the moving track of the reagent pipetting needle 1111 so that the reagent pipetting needle 1111 can move to the reagent washing submodule 71 for washing.

[0333] like Fig.10 and Fig.11 As shown, the luminescence detection device further includes a sample carrying portion 51, and the sample carrying portion 51 is used to carry a sample rack 511, and the sample rack 511 is used to carry a sample tube 52, and the sample to be detected is located in the sample tube 52. In this embodiment, when the sample rack 511 of the sample carrying portion 51 has multiple sample slots for storing multiple sample tubes, the sample rack 511 is configured as a linear structure, and the sample rack 511 is configured to be able to translate along a straight line, so that the sample on the sample carrying portion 51 can be smoothly obtained by the liquid adding module 1. In other embodiments, the sample rack 511 can also be arranged as a ring structure.

[0334] like Fig.10 and Fig.11 As shown, the luminescence detection device also includes a sample loading unit 112, which is used to add the sample in the sample tube 52 to the reaction cup 100 located in the incubation tray 81, for example, to the reaction cup 100 located in the sample adding station of the incubation module 8.

[0335] It should be noted that the order of adding the reagent and the sample is not limited. The reagent can be added to the reaction cup 100 first, and then the sample is added to the reaction cup 100. Alternatively, the sample can be added to the reaction cup 100 first, and then the reagent is added to the reaction cup 100.

[0336] like Fig.10 and Fig.11As shown, the sample loading unit 112 includes a sample pipetting needle 1112, a second lifting assembly 1121 for driving the sample pipetting needle 1112 to rise and fall, and a translation assembly 1122 for driving the sample pipetting needle 1112 to translate. Through the translation assembly 1122 and the second lifting assembly 1121, the sample pipetting needle 1112 can move back and forth between the sample tube 52 and the incubation tray 81, so as to add the sample in the sample tube 52 to the reaction cup 100 located in the incubation tray 81. In this embodiment, the second lifting assembly 1121 is preferably a vertical linear slide mechanism, and the translation assembly 1122 is preferably a horizontal linear slide mechanism, which can cover more incubation positions of the incubation module 8.

[0337] like Fig.14 As shown, in this embodiment, the translation assembly 1122 can cover multiple incubation positions 82 on the incubation tray 81. For example, when Fig.14 When the incubation plate 81 rotates clockwise, the translation component 1122 can move the sample pipette needle 1112 to above an incubation position 82 in the lower half. At this time, the sample pipette needle 1112 can add the sample to the reaction cup 100 to which the reagent has been added; the translation component 1122 can also move the sample pipette needle 1112 to above an incubation position 82 in the upper half. At this time, the sample can be added to the reaction cup 100 before the reagent.

[0338] like Fig.14 As shown, the washing module 7 also includes a sample washing submodule 72, which is used to wash the sample loading unit 112. Each time the sample loading unit 112 is used up, the sample pipetting needle 1112 of the sample loading unit 112 is cleaned so that the sample pipetting needle 1112 is clean when used next time to avoid sample contamination.

[0339] exist Fig.11 and Fig.14 In the embodiment, the sample washing submodule 72 is located between the sample holding portion 51 and the incubation tray 81 , and is located on the moving track of the sample pipetting needle 1112 , so that the sample pipetting needle 1112 can move into the sample washing submodule 72 .

[0340] like Fig.10 , 13 As shown in 14, the liquid adding module 1 includes a substrate loading submodule 12, which is used to load substrate liquid into the reaction cup. The liquid carrying module 5 includes a substrate liquid tank 54, and the substrate liquid tank 54 and the substrate loading submodule 12 are both arranged on one side of the incubation tray 81. The substrate liquid tank 54 is used to store the substrate liquid, and the substrate loading submodule 12 can move back and forth between the substrate liquid tank 54 and the incubation tray 81, so as to add the substrate in the substrate liquid tank 54 to the reaction cup 100. The specific structure of the substrate loading submodule 12 can refer to the first embodiment, and will not be repeated here.

[0341] like Fig.14 As shown, the luminescence detection device further includes a mixing module 4 , and the mixing module 4 in this embodiment is a rotating mixing module. The mixing module 4 is used to mix the sample and reagent in the reaction cup 100 .

[0342] like Fig.14 As shown, the mixing module 4 is located on one side of the incubation module 8 and on the moving track of the reaction cup transfer module 6. Specifically, in this embodiment, the mixing module 4 is located between the incubation tray 81 and the reaction cup temporary storage seat 801, and is located on the moving track of the reaction cup clamping assembly 66, so as to transfer the reaction cup 100 in the incubation tray 81 to the mixing module 4 through the reaction cup clamping assembly 66. In other words, the reaction cup transfer module 6 of this embodiment can not only transfer the unused reaction cups 100 in the reaction cup temporary storage module 80 to the incubation tray 81 of the incubation module 8, but also transfer the reaction cups 100 that have been added with reagents and samples in the incubation tray 81 of the incubation module 8 to the mixing module 4, and can also transfer the reaction cups 100 mixed by the mixing module 4 to the incubation tray 81 of the incubation module 8.

[0343] The mixing module 4 of this embodiment includes a rotating mixing submodule, which is used to drive the reaction cup 100 to rotate so that the reagent and the sample in the reaction cup 100 are mixed evenly.

[0344] like Fig.12 and Fig.14 As shown, the rotary mixing submodule of this embodiment includes a mixing motor 42 and a mixing seat 41 . The mixing motor 42 is used to drive the mixing seat 41 to rotate, and the mixing seat 41 is used to mount the reaction cup 100 .

[0345] The mixing motor 42 and the mixing seat 41 may be connected by belt transmission or other means.

[0346] One or more mixing positions may be provided on the mixing seat 41, and the reaction cup 100 may be placed in one of the mixing positions. Fig.14 In the embodiment, the mixing seat 41 is provided with two mixing positions.

[0347] like Fig.10 and Fig.14 As shown, the luminescence detection device further includes a detection module 3 , which is located on one side of the incubation tray 81 . When the reaction cup 100 moves to a position corresponding to the detection module 3 , the detection module 3 is used to detect the optical signal in the reaction cup 100 .

[0348] Alternatively, if Fig.10As shown, the detection module 3 includes a darkroom 31 and a detection submodule 32. The darkroom 31 can accommodate the reaction cup 100. The darkroom 31 can provide an environment required for detection, such as the environment in the darkroom 31 is darker than the external environment. More preferably, at least during detection, natural light from the outside cannot enter the darkroom 31. The detection submodule 32 is used to detect the optical signal in the reaction cup 100 in the darkroom 31. The detection submodule 32 includes a photomultiplier tube (i.e., a PMT detector), a photodiode (PD), an avalanche photodiode (APD), a silicon photomultiplier tube (SiPM), a complementary metal oxide semiconductor (CMOS) or a charge coupled device (CCD).

[0349] Optionally, the detection module 3 may include an entrance door and an exit door. When the reaction cup 100 enters the detection module 3, the entrance door and the exit door are opened, one reaction cup 100 is transferred out of the detection module 3, and another reaction cup 100 enters the detection module 3. When detecting, the entrance door and the exit door are both closed, thereby ensuring the darkroom 31 environment of the detection module 3.

[0350] like Fig.12 and Fig.14 As shown, the luminescence detection device further includes a cuvette recovery module 10 , which is located on the moving track of the cuvette clamping assembly 66 . The detected cuvette 100 can be transferred to the cuvette recovery module 10 through the cuvette clamping assembly 66 .

[0351] like Fig.12 and Fig.14 As shown, the reaction cup recovery module 10 includes a recovery track 10a and a recovery box 10b. The entrance of the recovery track 10a is located on the moving track of the reaction cup clamping assembly 66, and the exit of the recovery track 10a is connected to the recovery box 10b so that the reaction cup 100 enters the recovery box 10b.

[0352] like Fig.12 As shown, the recovery track 10a is tilted, and the entrance of the recovery track 10a is higher than the exit of the recovery track 10a, so that the reaction cup 100 located at the entrance of the recovery track 10a can move to the exit of the recovery track 10a under the action of gravity.

[0353] The reaction cup transfer module 6 of the present invention has a simple structure, and the reaction cup transfer module 6 can obtain the reaction cup 100 from the reaction cup temporary storage module 80 to the incubation module 8. After the reaction cup 100 is loaded with reagents, samples, and substrate solution in the incubation module 8, the reaction cup transfer module 6 transfers the reaction cup 100 to the mixing module 4 for mixing and then moves it back to the incubation module 8. Finally, the reaction cup transfer module 6 also transfers the detected reaction cup 100 to the reaction cup recovery module 10. That is, the reaction cup transfer module 6 realizes the transfer of the four processes of cup loading, mixing, incubation, and recovery of the reaction cup 100, making the entire luminescence detection device compact and efficient.

[0354] One use process of the luminescence detection device of this embodiment is as follows:

[0355] The cuvette 100 enters the cuvette arrangement submodule 92 from the storage submodule 91 of the cuvette storage module 9; the cuvette 100 enters the arrangement channel 921 of the cuvette arrangement submodule 92 and moves along the arrangement channel 921; the cuvette 100 falls out from the outlet of the arrangement channel 921 and enters the cuvette temporary storage tank of the cuvette temporary storage seat 801 of the cuvette temporary storage module 80;

[0356] The first motor 802 of the cuvette temporary storage module 80 rotates the cuvette temporary storage seat 801, so that the cuvette temporary storage slot containing the cuvette 100 coincides with the moving track of the cuvette clamping assembly 66 of the cuvette transfer module 6;

[0357] The first moving assembly 64 and the second moving assembly 65 of the cuvette transfer module 6 cooperate with each other to move the cuvette clamping assembly 66 to the top of the cuvette temporary storage tank; the cuvette clamping assembly 66 clamps the cuvette 100; the first moving assembly 64 and the second moving assembly 65 cooperate with each other to move the cuvette clamping assembly 66 to the top of one of the incubation positions 82 of the incubation tray 81 of the incubation module 8; the cuvette clamping assembly 66 releases the cuvette 100, and the cuvette 100 falls into the incubation position 82 of the incubation tray 81;

[0358] The incubation plate 81 rotates to make the reaction cup 100 coincide with the moving track of the reagent pipetting needle 1111 of the reagent loading module 113;

[0359] The first lifting component 1131 and the rotating component 1132 of the reagent loading module 113 cooperate with each other to move the reagent pipetting needle 1111 into the reagent bottle of the reagent compartment 53 so that the reagent pipetting needle 1111 absorbs the reagent. The first lifting component 1131 and the rotating component 1132 cooperate with each other to move the reagent pipetting needle 1111 into the reaction cup 100 so as to add the absorbed reagent into the reaction cup 100. The first lifting component 1131 and the rotating component 1132 cooperate with each other to move the reagent pipetting needle 1111 into the reagent washing submodule 71 so as to wash the reagent pipetting needle 1111.

[0360] The incubation plate 81 of the incubation module 8 rotates to make the reaction cup 100 coincide with the moving track of the sample pipetting needle 1112 of the sample loading module 112;

[0361] The second lifting assembly 1121 and the translation assembly 1122 of the sample loading module 112 cooperate with each other to move the sample pipette needle 1112 into the sample tube 52 of the sample rack 511 so that the sample pipette needle 1112 absorbs the sample. The second lifting assembly 1121 and the translation assembly 1122 cooperate with each other to move the sample pipette needle 1112 into the reaction cup 100 so as to add the absorbed sample into the reaction cup 100. The second lifting assembly 1121 and the translation assembly 1122 cooperate with each other to move the sample pipette needle 1112 into the sample washing submodule 72 so as to wash the sample pipette needle 1112.

[0362] The incubation plate 81 of the incubation module 8 rotates so that the reaction cup 100 containing the reagent and the sample coincides with the moving track of the reaction cup clamping assembly 66;

[0363] The first moving assembly 64 and the second moving assembly 65 of the reaction cup transfer module 6 cooperate with each other to move the reaction cup clamping assembly 66 to the top of the reaction cup 100, and the reaction cup clamping assembly 66 clamps the reaction cup 100. The first moving assembly 64 and the second moving assembly 65 cooperate with each other to move the reaction cup clamping assembly 66 to the mixing position; the mixing motor 42 drives the reaction cup 100 in the mixing position to rotate, so that the reagent and sample in the reaction cup 100 are evenly mixed; the first moving assembly 64 and the second moving assembly 65 cooperate with each other, and the reaction cup clamping assembly 66 puts the reaction cup 100 in the mixing position back to the incubation position 82;

[0364] The incubation plate 81 of the incubation module 8 rotates to make the reaction cup 100 containing reagents and samples coincide with the moving track of the substrate loading submodule 12 , and the substrate loading submodule 12 adds the substrate in the substrate liquid reservoir 54 to the reaction cup 100 .

[0365] The incubation plate 81 of the incubation module 8 rotates to rotate the mixed reaction cup 100 to a position aligned with the detection module 3; the detection module 3 transfers the reaction cup 100 to the detection module 3 and detects the liquid. After the detection is completed, the reaction cup 100 is transferred back to the incubation plate 81.

[0366] The incubation tray 81 of the incubation module 8 rotates so that the detected cuvette 100 coincides with the moving track of the cuvette clamping assembly 66 of the cuvette transfer module 6 , and the cuvette transfer module 6 moves the detected cuvette out of the incubation tray 81 .

[0367] The first moving component 64 and the second moving component 65 of the reaction cup transfer module 6 cooperate with each other to move the reaction cup clamping component 66 to the top of the reaction cup 100, and the reaction cup clamping component 66 clamps the reaction cup 100. The first moving component 64 and the second moving component 65 cooperate with each other to move the reaction cup clamping component 66 to the top of the recovery track 10a, and the reaction cup clamping component 66 releases the reaction cup 100, and the reaction cup 100 falls into the recovery track 10a and finally enters the recovery box 10b.

[0368] The luminescence detection device of the present invention can realize automatic detection and does not require a sample cleaning module, thereby greatly reducing the complexity of the luminescence detection device and the cost of the equipment.

[0369] The first sample result is faster, the fastest only takes 3 minutes, while the magnetic microparticle chemiluminescence takes 12 minutes. The luminescence detection device can greatly reduce the number of incubation positions on the incubation plate, greatly reducing the volume of the luminescence detection device. The structure is more compact and reasonable without the need for a sample cleaning module, and the test speed can reach 360T / H, while the magnetic microparticle chemiluminescence used in the luminescence detection device of the same volume in the prior art can only reach 80-120T / H.

[0370] The liquid carrying module 5 does not require stirring, has low cost and high reliability, and can store more reagents in a smaller volume.

[0371] The reaction cup transfer module 6 realizes the transfer of the four processes of cup loading, mixing, incubation and recovery of the reaction cup 100, and has a simple and compact structure.

[0372] The luminescence detection device of the present invention only needs one substrate chamber. After adding the substrate liquid, the reaction cup 100 can be directly sent to the detection module 3 for detection of luminescence signals, thereby reducing waiting time and accelerating detection speed.

[0373] Only three openings are required to connect the incubation tray 81 to the outside. Fewer openings greatly reduce the possibility of external light leaking into the darkroom, reduce the dark count of the network photomultiplier tube, and help improve the sensitivity of detection.

[0374] The luminescence detection device of the present invention directly tests the whole blood sample without the need for sample centrifugation, and cooperates with the sample automatic injection track to greatly improve the efficiency of the test.

[0375] Although the present invention has been described in detail above by means of general description, specific implementation methods and tests, it is obvious to those skilled in the art that some modifications or improvements may be made to the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention all fall within the scope of protection claimed by the present invention.

Claims

1. A luminescence detection device, characterized in that: include A detection module, the detection module is used to detect the optical signal in the reaction cup; An incubation module, wherein the incubation module is used to incubate the solution in the reaction cup; A reaction cup transfer module, wherein the reaction cup transfer module is used to transfer the reaction cup; A liquid adding module, which is used to add liquid required for detection into the reaction cup; a liquid carrying module, the liquid carrying module being used to carry at least one of a substrate liquid, a sample and a reagent; and A mixing module is used to mix the solution in the reaction cup.

2. The luminescence detection device according to claim 1, characterized in that: The liquid adding module can move in three-dimensional space.

3. The luminescence detection device according to claim 1, characterized in that: The mixing module mixes the solution in the reaction cup by moving.

4. The luminescence detection device according to claim 1, characterized in that: The mixing module includes at least one of an ultrasonic mixing submodule, a vibration mixing submodule and a rotation mixing submodule.

5. The luminescence detection device according to claim 4, characterized in that: When the mixing module includes the rotation mixing submodule, the rotation mixing submodule is connected to the reaction cup transfer module.

6. The luminescence detection device according to claim 1, characterized in that: At least one of the liquid adding module and the liquid carrying module is movable so as to transfer the substrate liquid, the sample and / or the reagent in the liquid carrying module.

7. The luminescence detection device according to claim 6, characterized in that: The liquid adding module and the liquid carrying module are capable of relative translation and relative vertical movement.

8. The luminescence detection device according to claim 6, characterized in that: The liquid carrying module can be moved in translation and / or in a vertical direction, and the liquid adding module can be moved in a vertical direction and / or in translation.

9. The luminescence detection device according to claim 1, characterized in that: The liquid adding module comprises a pipetting submodule and / or a substrate loading submodule. The pipetting submodule is used to load samples and / or reagents into the reaction cup; the substrate loading submodule is used to load substrate liquid into the reaction cup.

10. The luminescence detection device according to claim 9, characterized in that: The pipetting submodule and / or the substrate loading submodule can be moved to the top of the reaction cup, and / or the reaction cup transfer module can transfer the reaction cup to the bottom of the pipetting submodule and / or the substrate loading submodule; and / or the pipetting submodule and / or the substrate loading submodule can be moved to the top of the liquid carrying module.

11. The luminescence detection device according to claim 9, characterized in that: The pipetting submodule and / or substrate loading submodule comprises a pipetting needle, which can aspirate and release substrate liquid, sample and / or reagent.

12. The luminescence detection device according to claim 11, characterized in that: The liquid carrying module comprises a sample tube, and the pipette needle can pass through a tube cap of the sample tube to absorb the sample.

13. The luminescence detection device according to claim 11, characterized in that: The pipetting submodule and / or the substrate loading submodule comprises a liquid driving unit for driving the liquid flow.

14. The luminescence detection device according to claim 13, characterized in that: The liquid driving unit is a pump or a syringe.

15. The luminescence detection device according to claim 1, characterized in that: The luminescence detection device further comprises a washing module, and the washing module is used for washing the liquid adding module and / or the stirring module.

16. The luminescence detection device according to claim 15, characterized in that: The liquid adding module can be moved to the washing module, or the washing module can be moved to the liquid adding module.

17. The luminescence detection device according to claim 1, characterized in that: The reaction cup transfer module picks up the reaction cup by lifting, clamping and / or adsorption; and / or the reaction cup transfer module can transfer the reaction cup in three-dimensional space; and / or the reaction cup transfer module can rotate.

18. The luminescence detection device according to claim 1, characterized in that: The reaction cup transfer module can transfer the reaction cup to the incubation module.

19. The luminescence detection device according to claim 1, characterized in that: The incubation module includes an incubation tray, and the incubation tray is used to carry a reaction cup.

20. The luminescence detection device according to claim 19, characterized in that: The incubation tray is rotatable.

21. The luminescence detection device according to claim 20, characterized in that: The incubation module further comprises an incubation tray driving submodule, and the incubation tray driving submodule is used for driving the incubation tray to rotate.

22. The luminescence detection device according to claim 21, characterized in that: The incubation tray driving submodule comprises a driving member, a driving gear and a driven gear, wherein the driving member is connected to the driving gear and is used to drive the driving gear to rotate, and the driven gear is connected to the incubation tray and meshes with the driven gear.

23. The luminescence detection device according to claim 22, characterized in that: The incubation tray is annular, and the reaction cup transfer module is arranged on the inner circle or above the annular shape.

24. The luminescence detection device according to claim 23, characterized in that: The detection module is arranged inside or outside the incubation module.

25. The luminescence detection device according to claim 19, characterized in that: The incubation tray is also used to control the temperature of the reaction cup to be constant.

26. The luminescence detection device according to claim 1, characterized in that: The luminescence detection device further comprises a refrigeration module, and the refrigeration module is used to refrigerate at least one of the samples, reagents and substrates.

27. The luminescence detection device according to claim 1, characterized in that: The detection module includes a darkroom and a detection submodule, the darkroom can accommodate a reaction cup, and the detection submodule is used to detect the optical signal in the reaction cup in the darkroom.

28. The luminescence detection device according to claim 27, characterized in that: The detection submodule includes a photomultiplier tube.

29. The luminescence detection device according to claim 1, characterized in that: The liquid carrying module includes at least one of a sample carrying portion, a reagent chamber and a substrate liquid chamber, the sample carrying portion is used to carry samples, the reagent chamber is used to carry reagents, and the substrate liquid chamber is used to carry substrate liquid.

30. The luminescence detection device according to claim 29, characterized in that: The sample carrying portion is an annular structure, and the reagent chamber and the substrate liquid chamber are arranged inside or outside the annular structure.

31. The luminescence detection device according to any one of claims 1 to 30, characterized in that: The luminescence detection device further includes a cuvette storage module and / or a cuvette recovery module. The cuvette storage module is used to store unused cuvettes, and the cuvette recovery module is used to recover used cuvettes.

32. The luminescence detection device according to claim 31, characterized in that: The reaction cup transfer module can pick up the reaction cup in the reaction cup storage module, or the reaction cup storage module can transfer the stored reaction cup to a preset position, the reaction cup transfer module can pick up the reaction cup from the preset position, and / or the reaction cup transfer module can transfer the reaction cup to the reaction cup recovery module.

33. The luminescence detection device according to claim 31, characterized in that: The reaction cup storage module includes a storage submodule and a reaction cup arrangement submodule. The storage submodule is used to store reaction cups and provide reaction cups to the reaction cup arrangement submodule. The reaction cup arrangement submodule is used to arrange the reaction cups according to a preset arrangement rule.

34. The luminescence detection device according to claim 31, characterized in that: The liquid adding module, the detection module, the reaction cup storage module and / or the reaction cup recovery module are arranged on the side, upper part or lower part of the incubation tray.

35. The luminescence detection device according to claim 33, characterized in that: The reaction cup transfer module (6) comprises a first moving component (64), a second moving component (65) and a reaction cup clamping component (66); the execution end of the first moving component (64) can move along a first direction; the second moving component (65) is connected to the execution end of the first moving component (64) so ​​that the second moving component (65) can move along the first direction; the execution end of the second moving component (65) can move along a second direction different from the first direction; the reaction cup clamping component (66) is connected to the execution end of the second moving component (65) so that the reaction cup clamping component (66) can move along the second direction.

36. The luminescence detection device according to claim 35, characterized in that: The reaction cup recovery module (10) is located on the moving track of the reaction cup transfer module (6), and the detected reaction cup (100) is transferred to the reaction cup recovery module (10) through the reaction cup transfer module (6).

37. The luminescence detection device according to claim 36, characterized in that: The reaction cup recovery module (10) comprises: A recovery track (10a), the entrance of which is located on the moving track of the execution end of the reaction cup transfer module (6); and A recovery box (10b), wherein the recovery box (10b) is arranged at the exit of the recovery track (10a).

38. The luminescence detection device according to claim 35, characterized in that: The reaction cup arrangement submodule (92) comprises an arrangement channel (921) arranged obliquely, and the upper end of the arrangement channel (921) is connected to the storage submodule (91) for receiving the reaction cup (100) output from the storage submodule (91).

39. The luminescence detection device according to claim 35, characterized in that: The luminescence detection device also includes a reaction cup temporary storage module (80), which is arranged at the exit of the reaction cup arrangement submodule (92) and is located on the moving track of the reaction cup transfer module (6).

40. The luminescence detection device according to claim 39, characterized in that: The reaction cup temporary storage module (80) comprises a reaction cup temporary storage seat (801) arranged at the exit of the reaction cup arrangement submodule (92).

41. The luminescence detection device according to claim 40, characterized in that: The reaction cup temporary storage module (80) may further include a first motor (802), wherein the first motor (802) is used to drive the reaction cup temporary storage seat (801) to rotate so as to align the reaction cup temporary storage slot with the outlet of the reaction cup arrangement submodule (92).

42. The luminescence detection device according to claim 35, characterized in that: The mixing module (4) is located on the moving track of the reaction cup transfer module (6).

43. The luminescence detection device according to claim 1, characterized in that: The liquid carrying portion comprises a sample carrying portion (51), a reagent chamber (53) and a substrate liquid chamber (54); the sample carrying portion (51) is used to carry samples, the reagent chamber (53) is used to carry reagents, and the substrate liquid chamber (54) is used to carry substrate liquid.

44. The luminescence detection device according to claim 43, characterized in that: The reagent compartment (53) comprises a reagent table (531), on which a reagent placement portion (532) is provided, and a reagent bottle for containing a reagent can be placed on the reagent placement portion (532); the sample carrying portion (51) is provided with a sample rack (511), and the sample rack (511) is used to carry a sample tube (52).

45. The luminescence detection device according to claim 44, characterized in that: The sample rack is a linear structure.

46. ​​The luminescence detection device according to any one of claims 43 to 45, characterized in that: The liquid adding module comprises a pipetting submodule (11) and a substrate loading submodule (12), wherein the pipetting submodule comprises a sample loading unit (112) and a reagent loading unit (113), wherein the sample loading unit (112) is used to load a sample into a reaction cup, the reagent loading unit (113) is used to load a reagent into the reaction cup, and the substrate loading submodule (12) is used to load a substrate liquid into the reaction cup.

47. The luminescence detection device according to claim 46, characterized in that: The sample loading unit (112) comprises a sample pipetting needle (1112), a second lifting component (1121) for driving the sample pipetting needle (1112) to rise and fall, and a translation component (1122) for driving the sample pipetting needle (1112) to translate.

48. The luminescence detection device according to claim 47, characterized in that: The reagent loading unit (113) comprises a reagent pipetting needle (1111), a first lifting component (1131) for driving the reagent pipetting needle (1111) to rise and fall, and a rotating component (1132) for driving the reagent pipetting needle (1111) to rotate.

49. The luminescence detection device according to claim 46, characterized in that: The luminescence detection device further comprises a washing module (7), wherein the washing module (7) comprises a reagent washing submodule (71) for washing the reagent loading unit (113) and a sample washing submodule (72) for washing the sample loading unit (112).

50. The luminescence detection device according to claim 1, characterized in that: The reaction cup transfer module (6) comprises a first rotating drive member, a first rotating arm (61), a second rotating drive member, a second rotating arm (62) and a pick-up and place member (63) which are connected in sequence, wherein the first rotating drive member drives the first rotating arm (61) to rotate, the second rotating drive member drives the second rotating arm (62) to rotate, and the pick-up and place member (63) realizes the functions of lifting, clamping and / or adsorption.

51. A multi-detection index analysis system, characterized in that: comprising at least one of a biochemical analysis module, a blood cell analysis module and a blood coagulation analysis module and a luminescent detection device according to any one of claims 1 to 50; When the multi-detection index analysis system includes at least one of the liquid adding module, the liquid carrying module, the incubation module and the washing module and the biochemical analysis module, the biochemical analysis module matches and uses at least one of the liquid adding module, the liquid carrying module, the incubation module and the washing module of the luminescence detection device; When the multi-detection index analysis system includes at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module, the recovery module, the reaction cup storage module and the washing module and the blood coagulation analysis module, the blood coagulation analysis module matches and uses at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module, the recovery module, the reaction cup storage module and the washing module of the luminescent detection device; When the multi-detection index analysis system includes at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module and the washing module and the blood cell analysis module, the blood cell analysis module matches and uses at least one of the liquid adding module, the liquid carrying module, the incubation module, the mixing module and the washing module of the luminescence detection device.

52. The multi-detection index analysis system according to claim 51, characterized in that: When the multi-detection index analysis system includes the biochemical analysis module, the incubation module includes an outer incubation tray and an inner incubation tray, the outer incubation tray is a ring-shaped structure, the reaction cup carried by the outer incubation tray contains one of the solution to be biochemically analyzed and the solution to be homogeneously luminescent detected, and the reaction cup carried by the outer incubation tray contains another of the solution to be biochemically analyzed and the solution to be homogeneously luminescent detected.

53. The multi-detection index analysis system according to claim 52, characterized in that: The outer incubation tray and / or the inner incubation tray may be rotatable.

54. A detection method, characterized in that: The luminescence detection device according to any one of claims 1 to 50 performs the detection method, and the detection method comprises: Detection: Place the reaction cup on the detection module, and the detection module detects the test liquid in the reaction cup.

55. The detection method according to claim 54, characterized in that Prior to the detection, the method further includes: Preparation of the test liquid: The liquid adding module adds the liquid required for the test into the reaction cup, so that the liquid forms the test liquid in the reaction cup.

56. The detection method according to claim 55, characterized in that Before the detection of the test liquid, the method comprises: Transferring the reaction cup: the reaction cup transfer module or the incubation module transfers the reaction cup to the detection module.

57. The detection method according to claim 56, characterized in that The preparation of the test solution comprises: Adding sample: the liquid adding module adds the sample to the reaction cup; and / or, Adding reagents: the liquid adding module adds reagents to the reaction cup; and / or, Adding substrate liquid: the liquid adding module adds the substrate liquid into the reaction cup.

58. The detection method according to claim 57, characterized in that After adding the reagent and / or after adding the substrate solution, the method further comprises: Mixing: The mixing module mixes the liquid in the reaction cup; and / or, After adding the sample and / or after adding the reagent and / or after adding the substrate solution, the method further comprises: Cleaning: The washing module cleans the liquid adding module and / or the stirring module.

59. The detection method according to claim 58, characterized in that Before adding the sample, the method further includes: Taking samples: the liquid adding module moves to the liquid carrying module, and / or the liquid carrying module moves to the liquid adding module, and the liquid adding module absorbs the sample in the liquid carrying module; And / or before adding the reagent, the method further comprises: Taking reagents: the liquid adding module moves to the liquid carrying module, and / or the liquid carrying module moves to the liquid adding module, and the liquid adding module absorbs the reagents in the liquid carrying module.

60. The detection method according to claim 59, characterized in that The added sample includes: The reaction cup transfer module transfers the reaction cup to the bottom of the liquid adding module; or the liquid adding module moves to the top of the reaction cup, and the liquid adding module adds the sample to the reaction cup; or the incubation module transfers the reaction cup to the bottom of the liquid adding module; and / or The reagent addition comprises: The reaction cup transfer module transfers the reaction cup to the bottom of the liquid adding module; or the liquid adding module moves to the top of the reaction cup, and the liquid adding module adds the reagent to the reaction cup; the incubation module transfers the reaction cup to the bottom of the liquid adding module; and / or The substrate liquid addition comprises: The reaction cup transfer module transfers the reaction cup to the bottom of the liquid adding module; or the liquid adding module moves to the top of the reaction cup, and the liquid adding module adds the substrate liquid to the reaction cup; the incubation module transfers the reaction cup to the bottom of the liquid adding module.

61. The detection method according to claim 60, characterized in that The detection also includes: Incubation: The cuvette is incubated at the preset temperature of the incubation module for the preset time.

62. The detection method according to claim 61, characterized in that Prior to the detection, the method further includes: Providing a reaction cup: the reaction cup transfer module picks up the reaction cup provided by the reaction cup storage module or the reaction cup storage module provides the reaction cup to the incubation module; And / or, the detection further includes: Recycling reaction cup: The reaction cup transfer module transfers the reaction cup to the reaction cup recycling module.

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