Nano medicament for resisting soybean mosaic virus and preparation method thereof

Abstract

The invention discloses an anti-soybean mosaic virus nano medicament and a preparation method thereof. The anti-soybean mosaic virus nano medicament is synthesized from a xinjunan solution, SPc powder and a solvent, wherein the weight ratio of the SPc powder to the xinjunan solution is 1: (0.5-3.0); the solvent is clear water, purified water and deionized water. The invention relates to a self-assembled nano antiviral pesticide based on SPc and a broad-spectrum bactericide / antiviral agent xinjunan, which is used for preventing and treating SMV in the field. The SPc is self-assembled with the xinjunan through hydrogen bonds and Van der Waals force, the particle size of the xinjunan is remarkably reduced, the contact angle of the xinjunan on soybean leaf surfaces is reduced, the leaf surface retention is improved, and excellent wettability and adhesion performance are shown. Besides, the nano-agent obviously inhibits accumulation of viruses in soybean plants, so that the maturing rate of soybean fruits is increased, the mottle rate of seed coats is reduced, and the field control effect on SMV is greatly enhanced.

Description

Technical Field

[0001] The invention relates to the field of pesticides, and more particularly to a nanometer agent used for preventing and controlling soybean mosaic virus on soybean economic crops. Background Art

[0002] Soybean is the most important leguminous crop in the world and the main source of high-quality plant protein and edible oil in the human diet. Soybean mosaic virus (SMV) belongs to the largest genus of plant pathogenic viruses in the family Potyviridae and is the most common and destructive viral pathogen in all soybean producing areas around the world. It can cause soybean leaf mosaic or necrosis, plant stunting, and seed coat mottles, which can lead to an 8% to 35% yield reduction under natural field conditions, and in severe cases can even cause crop failure.

[0003] Star cationic polymer (SPc) is a simple, low-cost, and environmentally friendly nanocarrier. The SPc powder structure formula is based on the article "AFacile-Synthesized Star Polycation Constructed as a Highly Efficient Gene Vector in Pest Management" published in ACS Sustainable Chemistry & Engineering on February 13, 2019, by Jianhao Li. The SPc powder structure is as follows Figure 1 shown.

[0004] The main component of the mother drug (condensation liquid) of cintamidine (formerly registered as Junduqing) is di-n-octyldiethylenetriamine. cintamidine is a low-toxic, broad-spectrum alkyl polyamine fungicide developed in my country. It has killing and inhibitory effects on a variety of plant fungi, bacteria and viruses that cause crop diseases. It has a strong inhibitory effect on the mycelial growth and spore germination of pathogens. It can destroy the cell membrane of pathogens, inhibit the respiratory system, coagulate proteins, denature enzymes, and have antibacterial and bactericidal effects. Although cintamidine has a certain inhibitory effect on bacteria and viruses. However, when cintamidine is used in open field prevention, the prevention and control effect of plant viral diseases is poor, and there is no report on cintamidine on soybean mosaic virus.

[0005] At present, there is no very effective drug for the prevention and control of SMV. We should adhere to the principle of "prevention first, comprehensive prevention and control", strengthen seed quarantine, and adopt a comprehensive prevention and control strategy that focuses on agricultural prevention and control, and combines biological control with chemical control and genetic engineering technology to ensure high-quality, high-yield and stable soybean production. First of all, quarantine of soybean seeds should be strengthened to avoid the initial infection source of SMV. In terms of agricultural prevention and control, appropriate disease-resistant varieties should be selected according to the conditions of different soybean producing areas, and reasonable rotation should be carried out in soybean planting areas to strengthen field management. After soybean harvest, the fields should be cleaned in time, deep plowing and drying should be carried out to reduce the amount of overwintering bacteria. In terms of biological control, soybean aphids are important vector insects for the spread of SMV. The rampage of soybean aphids can be controlled by releasing natural enemy insects, so as to effectively control SMV. In terms of chemical control, appropriate antiviral agents should be selected, disease monitoring should be strengthened, and precise application of drugs should be carried out according to field conditions. In terms of genetic engineering, the viral capsid protein that can inhibit the decapping of the virus is introduced into soybeans, and the viral capsid protein produced by it is used to inhibit the replication of the virus to achieve the purpose of preventing and controlling SMV. Summary of the invention

[0006] One of the purposes of the present invention is to design a nano-agent for resisting soybean mosaic virus. It is a self-assembled nano-sphenanthene / SPc complex based on a star-shaped cationic polymer and a broad-spectrum fungicide / antiviral agent sphenanthene, and is a nano-antiviral pesticide for controlling soybean mosaic virus (SMV) in the field. The nano-agent for resisting soybean mosaic virus is synthesized from sphenanthene solution, SPc powder and solvent; wherein the weight ratio of SPc powder to sphenanthene solution is 1:0.5-3.0; and the solvent is clean water, purified water, and deionized water.

[0007] The structural formula of the nanoparticle agent against soybean mosaic virus of the present invention is

[0008] The anti-soybean mosaic virus nanoparticles of the present invention can be prepared into a pharmaceutically aqueous solvent or a pharmaceutically acceptable solution dosage form by a general method.

[0009] The second object of the present invention is to propose a method for preparing a nanopharmaceutical for resisting soybean mosaic virus. SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to form a spherical nano-succinil / SPc complex, significantly reducing the particle size of succinil and effectively improving the adhesion and deposition of the agent on the leaves of soybean plants. The preparation of the nanopharmaceutical is to mix SPc powder and succinil solution in a solvent at a temperature of 15°C to 35°C, incubate for 5 to 30 minutes, and obtain the nanopharmaceutical by self-assembly of SPc with succinil through hydrogen bonds and van der Waals forces.

[0010] The third object of the present invention is to design a dosage and method for controlling soybean mosaic virus in the field. The nano-sintrafenid / SPc complex reduces the contact angle of sintrafenid on the soybean leaf surface, increases the drug retention on the leaf surface, exhibits excellent wettability and adhesion properties, and significantly improves the transfer performance of sintrafenid in soybean plants. In addition, the nano-sintrafenid / SPc complex significantly inhibits the accumulation of viruses in soybean plants, thereby increasing the soybean fruit setting rate, reducing the seed coat mottle rate, and greatly enhancing the field control effect of SMV.

[0011] In general, the self-assembled nano antiviral pesticide with strong adhesion performance of the present invention, on the one hand, uses SPc to load succinimid to reduce the particle size of succinimid; on the other hand, it increases the application of succinimid on soybean mosaic virus; thirdly, it will contribute to new strategies and methods for effectively controlling SMV in the field, and help reduce the economic losses of soybeans caused by yield losses and reduced seed quality.

[0012] The technical effects of the present invention are:

[0013] ① For the first time, a self-assembled nano anti-soybean mosaic virus pesticide based on star-shaped cationic polymer (SPc) and broad-spectrum fungicide / antiviral agent fenpyroxetine, namely nanofenpyroxetine / SPc complex, was reported. Loading fenpyroxetine with SPc can effectively reduce the particle size of fenpyroxetine and promote the deposition and penetration of fenpyroxetine on target organisms.

[0014] ② The preparation method is simple. At room temperature, cincomycin and SPc powder can spontaneously combine in aqueous solution through hydrogen bonds and van der Waals forces to form a spherical nano-cincomycin / SPc complex, thereby assembling to form a nano anti-soybean mosaic virus pesticide.

[0015] ③ The prepared nano-sphenanthracene / SPc complex has a smaller particle size (particle size is 183nm-307nm), and has better leaf wettability and surface adhesion. Its leaf contact angle is also reduced, and the drug retention is significantly increased.

[0016] ④The nanosphenanthamide / SPc complex significantly inhibited the accumulation of SMV virus in soybean plants.

[0017] ⑤ The nano-sphenanthamide / SPc complex effectively reduced the disease index of SMV, improved the relative field control effect against SMV, and helped reduce the field usage of sphenanthamide.

[0018] ⑥ Nano-sintracin / SPc complex greatly improved the agronomic traits of soybean plants in the field. It helps to improve the plant height of soybean plants infected with SMV, increase the number of nodes and pods of soybean plants, increase the number of grains per plant, and significantly reduce the soybean seed coat mottle rate. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is the SPc structural formula of the carrier selected in the present invention.

[0020] Figure 2 The synthetic route diagram of the present invention for preparing the nanometer medicine for resisting soybean mosaic virus is shown in the rectangular frame, wherein SPc is used to load cinclofenac.

[0021] Figure 3 This is a diagram of the formation mechanism of the nanoparticle drug of Example 1 obtained by the method of the present invention.

[0022] Figure 4 The figure is a morphological diagram of the nanometer medicine for resisting soybean mosaic virus prepared by the method of the present invention.

[0023] Figure 5 The contact angle of the nanoparticles of Example 1 prepared by the method of the present invention on soybean leaves.

[0024] Figure 6 It is the retention amount of the nano-agent of Example 1 prepared by the method of the present invention on soybean leaves.

[0025] Figure 7 It is the relative accumulation amount of SMV virus in soybean plants treated with the nanoparticle agent of Example 1 prepared by the method of the present invention at different time points.

[0026] Figure 8 This is a graph showing the improvement in soybean plant height by the nanoparticle agent of Example 1 prepared by the method of the present invention.

[0027] Fig. 9 This is a graph showing the results of increasing the number of plant nodes using the nanoparticles of Example 1 prepared by the method of the present invention.

[0028] Fig.10 This is a graph showing the results of increasing the number of pods per plant using the nanoparticles of Example 1 prepared by the method of the present invention.

[0029] Fig.11 This is a graph showing the results of increasing the number of particles per plant of the nanoparticles of Example 1 prepared by the method of the present invention.

[0030] Fig.12 This is a graph showing the results of reducing the seed coat mottle rate of the nanoparticle agent of Example 1 prepared by the method of the present invention. DETAILED DESCRIPTION

[0031] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. The various parameter demonstrations listed are only preferred embodiments of the present invention. It should be noted that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention.

[0032] Example 1, nanopharmaceutical with a ratio of 1:1 of SPc:sintramil

[0033] Dosage: The weight ratio of SPc powder to succinimidyl solution is 1:1. The mass percentage concentration of succinimidyl solution in the nanoparticle is 0.05 mg / mL.

[0034] SPc powder and succinil solution were mixed in deionized water by magnetic stirring at a temperature of 25°C and incubated for 15 minutes. SPc self-assembled with succinil through hydrogen bonding and van der Waals forces to obtain a nanopharmaceutical based on a star-shaped cationic polymer and a broad-spectrum bactericide / antiviral agent succinil, a self-assembled nano-succinil / SPc complex, such as Figure 2 The rectangular box shown shows the structural formula.

[0035] The cintamidine was selected from the cintamidine acetate produced by Jiangsu Dongbao Agrochemical Co., Ltd. The cintamidine concentration was 1.2% (mass percentage concentration). The chemical structural formula of cintamidine is The peak area ratio (%) is 57.1. Figure 2 The chemical structure of cintamidine is simplified to HN-·. The structural formula of the nanoparticle against soybean mosaic virus is

[0036] like Figure 2 The synthesis steps of Example 1 shown are: pouring SPc powder and styrac amine solution into a beaker filled with deionized water, placing two clean magnets in the beaker and placing it on a magnetic stirrer for stirring at a stirring speed of 1200 rpm. After being evenly mixed, incubating in a room at a temperature of 25°C for 15 minutes to obtain a self-assembled nano-styrac amine / SPc complex of an anti-soybean mosaic virus pesticide. The synthetic route of the target product describes the preparation of the nano-styrac amine / SPc complex of the present invention, and all raw materials are prepared by the methods described in these schematic diagrams, by methods well known to those of ordinary skill in the field of organic chemistry, or purchased commercially. All final products of the present invention are prepared by the methods described in these schematic diagrams or by methods similar thereto, which are well known to those of ordinary skill in the field of organic chemistry.

[0037] In order to study the formation mechanism of the nano antiviral agent in Example 1, the binding mechanism of the nanocarrier SPc and sphenanthracene (weight ratio of 1:1) was analyzed by isothermal titration calorimetry (ITC). The experiment used Nano ITC (TAInstruments Waters, USA) and NanoAnalyze software for ITC analysis.

[0038] It can be seen from the ITC results that the cintamidine / SPc complex of Example 1 can achieve self-assembly. A relatively high affinity constant Ka (1.530×10 5 M-1 ) and a lower dissociation constant Kd (6.534×10 -6 M) showed that there was a strong interaction between SPc and sphenanthracene, while the negative ΔG (Gibbs free energy) indicated that the interaction was spontaneous. The ΔH (enthalpy change) value of -99.93 kJ / mol and the ΔS (entropy change) value of -235.9 J / mol·K indicated that the self-assembly was mainly driven by hydrogen bonds and van der Waals forces, such as Figure 3 As shown. Figure 1 and Figure 2 Compare and Figure 3 Performance analysis, the obtained product of Example 1 and the prior art Figure 1 The connection mode of the side chains in the SPc powder structural formula is different. After synthesis, the two structures are obviously different, resulting in different ways and categories of biological activity exerted by the preparation method. Therefore, it cannot be considered that the product of Example 1 is an inventive concept that can be obtained by conventional selection and replacement of substituent groups.

[0039] (a) Morphological characterization of nanopharmaceuticals

[0040] The particle size and morphology of the nanoparticles were determined using a nanoparticle sizer and a scanning electron microscope. A nanoparticle sizer (Malvern Instruments Ltd., UK) was used to measure the particle size of the nanoparticles obtained in Example 1. During the test, 3 mL was drawn into a quartz glass dish with a pipette and placed in the sample slot of the nanoparticle sizer for particle size detection. Each sample was tested 3 times. The scanning electron microscope sample was prepared as follows: On a sample stage with a single crystal silicon wafer attached, 10 μL of each of the two solutions was drawn with a pipette and dropped on the single crystal silicon wafer. After natural drying at room temperature, the sample stage was placed in a gold spraying instrument for gold spraying. After the gold spraying was completed, the sample stage was placed in a scanning electron microscope (Regulus 8100, Japan) to observe the morphology, as shown Figure 4 As shown in the figure, SPc can reduce the particle size of cintamidine from 334nm ( Figure 4 (A)) is significantly reduced to 229nm ( Figure 4 (B)), and the nano antiviral agents are mainly nearly spherical, and the particle size also decreases with the addition of SPc, and the two results are consistent.

[0041] (b) Interface characteristics of nanoparticles

[0042] The contact angles of the two solutions on soybean leaves were observed using a dynamic contact angle meter. With the addition of SPc, the contact angle of sinotriptyline on soybean leaves decreased significantly. The average contact angle of the agent in Example 1 was 84.07°, which was significantly smaller than that of sinotriptyline alone (94.84°), indicating that the wetting performance of nanosinotriptyline on soybean leaves was significantly improved. Figure 5 shown.

[0043] Cut the soybean leaves into squares with a side length of 2 cm and weigh them on an analytical balance. The mass is recorded as M. 1 The leaves were completely immersed in the two prepared solutions. After 15 seconds, the leaves were taken out and weighed after no water dripped. The mass was recorded as M. 2 , calculate the retention Calculate the retention of Example 1 on soybean leaves. The nano antiviral agent has excellent leaf adhesion performance. The retention of sinotriptyline on soybean leaves is 11.18 mg / cm 2 After nano-crystallization, the retention amount increased to 13.44 mg / cm 2 , the retention rate is significantly improved, such as Figure 6 shown.

[0044] (c) Field efficacy of nanopharmaceuticals

[0045] The soybean plants were inoculated with SMV virus. One week later, the inoculated soybean plants were sprayed with deionized water, cintofenamide and the agent of Example 1, respectively. The control group was deionized water. After an interval of one week, the second spraying was carried out, and the total spraying was 2 times. 1st DPA) and 7 days after the second spraying (7 2nd The virus symptoms (i.e., asymptomatic, mosaic, and necrosis) on soybean leaves were visually observed and recorded at 14:00 p.m. (DPA), the number of diseased plants was counted, and each plant was graded according to the disease grade (0-4). Then, the disease incidence rate (DR), disease index (DI), and relative control effectiveness (RCE) of each treatment method at different time points were calculated using the following formulas, where SD represents standard deviation.

[0046] (i)

[0047] (ii)

[0048] (iii)

[0049] One week after inoculation with SMV (0 DPA), the disease index of leaves treated with deionized water was the highest among all preparations and was 1st DPA and 7 2nd DPA showed an increasing trend. For the treatment with cintamidine and the agent of Example 1, at 7 2nd When treated with DPA, the disease index was significantly reduced, and the disease index after treatment with the agent of Example 1 was 31.96%, which was significantly lower than 36.97% after treatment with cintamidine.

[0050] The investigation of each treatment in 7 1st DPA and 7 2ndThe relative control effect at DPA time point showed that the control effect of the agent treatment in Example 1 on SMV was the best among all treatments. Compared with the treatment with cintamidine, the control effect of Example 1 on SMV was the best at 7 1st DPA has a significant improvement in prevention effect. 2nd The best control effect was 31.92% at DPA, which was much higher than that of sinotriptyline (21.24%). The relative control effect results of each treatment at each time point showed the synergistic effect of the nano antiviral agent system on the agent, indicating that the use of the nano antiviral agent system can improve the control effect of SMV.

[0051]

[0052] (d) Inhibition of SMV virus by nanoparticles

[0053] Virus accumulation in SMV-infected soybean plants was detected by qRT-PCR. Gene-specific forward primers targeting the viral CP gene were designed using Primer Premier 5.0 software.

[0054] (5′-CAGATGGGCGTGGTTATGA-3′) and reverse primer

[0055] (5′-ACAATGGGTTTCAGCGGATA-3′).

[0056] GmTubulin (accession number AY907703) amplified by the reverse primer (5′-GGAGTTCACAGAGGCAGAG-3′) and the reverse primer (5′-CACTTACGCATCACATAGCA-3′) was used as the internal reference gene. 1st DPA and 7 2nd Deionized water, cintamidine and soybean leaves treated with the agent of Example 1 were collected by DPA. RNA Simple Total RNA Kit (Tiangen, China) and Total RNA extraction and first-strand cDNA synthesis were performed using RT Master Mix (Takara, Japan). Premix Ex Taq TM qRT-PCR was performed and relative quantification method (2 -ΔΔCt ) Quantify the transcription level of CP gene.

[0057] The infected soybean plants under the three treatments were randomly sampled at different time points, and the SMV expression levels in the plants under each treatment were detected by qRT-PCR to analyze the virus accumulation. Figure 7 The results show that for 7 1stDPA and 7 2nd At the DPA time point, the soybean plants treated with deionized water had the highest virus accumulation, followed by the soybean plants treated with cintamidine, and the soybean plants treated with the agent in Example 1 had the lowest virus accumulation. 2nd The relative expression of SMV was suppressed to about 0.35 at DPA time point, which was much better than 0.89 of the treatment with cintamidine. 2nd During the DPA period, the virus accumulation of soybean plants treated in Example 1 continued to decrease significantly, indicating that the nano antiviral agent can significantly inhibit the expression and accumulation of SMV.

[0058] (e) Nanoparticles improve agronomic traits of soybean plants infected with SMV

[0059] After the soybeans were harvested, the soybean plants treated with deionized water, cintamidine and the agent of Example 1 were investigated and analyzed for agronomic traits, and five indicators were selected for agronomic traits, including plant height, number of nodes, number of pods per plant, number of grains per plant and seed coat mottle rate. For the seed coat mottle rate, 100 different seeds were randomly selected for each treatment for three times for statistics. For the remaining four evaluation traits, 10 plants were randomly selected for each treatment for statistics and analysis.

[0060] In field studies, the height of plants ( Figure 8 As shown), number of sections ( Fig. 9 Number of pods per plant ( Fig.10 Number of seeds per plant ( Fig.11 shown) and seed coat mottle rate ( Fig.12 The nano antiviral agent was used to evaluate the effect of improving the agronomic traits of soybean plants infected with SMV. Figure 8 to Figure 12 In the experiment, deionized water was used as a control. The treatment with cintamidine and Example 1 had certain improvement effects on the plant height, number of nodes, number of pods per plant, number of grains per plant and seed coat mottle rate of soybeans. Among them, the improvement of the number of pods per plant and the number of grains per plant and the control of the seed coat mottle rate were very significant. After treatment with Example 1, the average number of pods per plant of soybeans was 40.5, the average number of grains per plant was 100.1, and the seed coat mottle rate was only 7.67%; after treatment with cintamidine, the average number of pods per plant of soybeans was 32.4, the average number of grains per plant was 79.2, and the seed coat mottle rate was 20.67%; the treatment effect of Example 1 was significantly better than that of cintamidine. These results collectively reveal that nano-antiviral agents play a positive role in reducing yield losses and improving seed quality in soybean production.

[0061] Example 2, nanopharmaceutical with SPc: cintamidine at a ratio of 1:0.5

[0062] Dosage: The weight ratio of SPc powder to succinimidyl solution is 1:0.5. The mass percentage concentration of succinimidyl solution in the nanoparticle is 0.05 mg / mL.

[0063] The SPc powder and the succinil solution are stirred and mixed in deionized water at a temperature of 22°C (the stirring tool is not limited, and the mixing is preferably uniform), and incubated for 30 minutes. SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to obtain a nanopharmaceutical based on a star-shaped cationic polymer and a broad-spectrum bactericide / antiviral agent succinil self-assembled nano-succinil / SPc complex, such as Figure 2 The rectangular box shown shows the structural formula.

[0064] The nanoparticles prepared in Example 2 have a nearly spherical shape and a particle size of 183 nm (e.g. Figure 4 The retention amount on soybean leaves was 14.13 mg / cm 2 The relative control efficiency in the field was 29.47%, and the relative accumulation of SMV was suppressed to 0.48.

[0065] Example 3, nanopharmaceutical with a ratio of SPc: cintamidine of 1:3

[0066] Dosage: The weight ratio of SPc powder to succinimidyl solution is 1:3. The mass percentage concentration of succinimidyl solution in the nanoparticle is 0.025 mg / mL.

[0067] The SPc powder and the succinil solution are stirred and mixed in deionized water at a temperature of 25°C (the stirring tool is not limited, and the mixing is preferably uniform), and incubated for 10 minutes. SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to obtain a nanopharmaceutical based on a star-shaped cationic polymer and a broad-spectrum bactericide / antiviral agent succinil self-assembled nano-succinil / SPc complex, such as Figure 2 The rectangular box shown shows the structural formula.

[0068] The nanoparticles prepared in Example 3 have a nearly spherical shape and a particle size of 307 nm. The retention on soybean leaves is 11.34 mg / cm 2 The relative control effect in the field was 22.56, and the relative accumulation of SMV was suppressed to 0.81.

[0069] Example 4, nanopharmaceutical with a ratio of SPc: cintamidine of 1:2

[0070] Dosage: The weight ratio of SPc powder to succinimidyl solution is 1:2. The mass percentage concentration of succinimidyl solution in the nanoparticle is 0.10 mg / mL.

[0071] The SPc powder and the succinil solution are stirred and mixed in deionized water at a temperature of 20°C (the stirring tool is not limited, and the mixing is preferably uniform), and incubated for 10 minutes. SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to obtain a nanopharmaceutical based on a star-shaped cationic polymer and a broad-spectrum fungicide / antiviral agent succinil. The self-assembled nano-succinil / SPc complex is a nanopharmaceutical, such as Figure 2 The rectangular box shown shows the structural formula.

[0072] The nanoparticles prepared in Example 4 have a nearly spherical shape and a particle size of 284 nm. The retention on soybean leaves is 11.65 mg / cm 2 The relative control efficiency in the field was 24.72%, and the relative accumulation of SMV was suppressed to 0.73.

[0073] Example 5, nanopharmaceutical with SPc: cintamidine at a ratio of 1:1.5

[0074] Dosage: The weight ratio of SPc powder to succinimidyl solution is 1:1.5. The mass percentage concentration of succinimidyl solution in the nanoparticle is 0.08 mg / mL.

[0075] The SPc powder and the succinil solution are stirred and mixed in deionized water at a temperature of 15°C (the stirring tool is not limited, and the mixing is preferably uniform), and incubated for 25 minutes. SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to obtain a nanopharmaceutical based on a star-shaped cationic polymer and a broad-spectrum bactericide / antiviral agent succinil self-assembled nano-succinil / SPc complex, such as Figure 2 The rectangular box shown shows the structural formula.

[0076] The nanoparticles prepared in Example 5 have a nearly spherical shape and a particle size of 267 nm. The retention on soybean leaves is 11.83 mg / cm 2 The relative control efficiency in the field was 28.63%, and the relative accumulation of SMV was suppressed to 0.56.

[0077] Example 6, nanopharmaceutical with a ratio of SPc: cintamidine of 1:1

[0078] Dosage: The weight ratio of SPc powder to succinimidyl solution is 1:1. The mass percentage concentration of succinimidyl solution in the nanoparticle is 0.10 mg / mL.

[0079] The SPc powder and the succinil solution are stirred and mixed in deionized water at a temperature of 35°C (the stirring tool is not limited, and the mixing is preferably uniform), and incubated for 5 minutes. SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to obtain a nanopharmaceutical based on a star-shaped cationic polymer and a broad-spectrum bactericide / antiviral agent succinil self-assembled nano-succinil / SPc complex, such as Figure 2 The rectangular box shown shows the structural formula.

[0080] The nanoparticles prepared in Example 6 have a nearly spherical shape and a particle size of 295 nm. The retention on soybean leaves is 11.51 mg / cm 2 The relative control efficiency in the field was 27.59%, and the relative accumulation of SMV was suppressed to 0.67.

Claims

1. A nanopharmaceutical for resisting soybean mosaic virus, characterized in that: The nanometer medicine for resisting soybean mosaic virus is synthesized from acinamide solution, SPc powder and solvent; wherein the weight ratio of SPc powder to acinamide solution is 1:0.5-3.0; and the solvent is clean water, purified water or deionized water.

2. The anti-soybean mosaic virus nanoparticle according to claim 1, characterized in that: The mass percentage concentration of the sinopyridamole solution in the nanomedicine is 0.01-0.10 mg / mL.

3. The anti-soybean mosaic virus nanoparticle according to claim 1, characterized in that: The weight ratio of SPc powder to sphenanthracene solution is 1:1.5-2.

0.

4. The anti-soybean mosaic virus nanoparticle according to claim 1 or 2, characterized in that: The weight ratio of SPc powder to succinimidyl solution is preferably 1:1, and the mass percentage concentration of succinimidyl solution in the nanopharmaceutical is 0.05 mg / mL; The chemical structure of cintamidine is The peak area ratio (%) was 57.

1.

5. The anti-soybean mosaic virus nanoparticle according to claim 1 or 2, characterized in that: The structural formula of the nanoparticles against soybean mosaic virus is 6. The anti-soybean mosaic virus nanopharmaceutical according to claim 1 or 2, characterized in that: The nanopharmaceutical is prepared as a pharmaceutical aqueous solvent.

7. The anti-soybean mosaic virus nanoparticle according to claim 1 or 2, characterized in that: The invention discloses an application of active ingredients of nanopharmaceuticals in preparing pesticides for treating and / or preventing soybean mosaic virus.

8. The anti-soybean mosaic virus nanoparticle according to claim 1 or 2, characterized in that: The application of active ingredients of nano-agents in the preparation of soybean mosaic virus for preventing and controlling soybeans in the field.

9. The method for preparing the anti-soybean mosaic virus nanoparticle agent according to claim 1, characterized in that: Firstly, the dosage is prepared, the weight ratio of SPc powder to succinil solution in the nano-agent is 1:0.5-3.0, and the mass percentage concentration of succinil solution is 0.01-0.10 mg / mL; then, the SPc powder and the succinil solution are uniformly mixed in a solvent at a temperature of 15°C-35°C, and incubated for 5-30 minutes. During the synthesis process, SPc self-assembles with succinil through hydrogen bonds and van der Waals forces to obtain the nano-agent.

10. The method for preparing an anti-soybean mosaic virus nanoparticle according to claim 5, characterized in that: The mixing of SPc powder and sphenanthracene solution in the solvent is carried out on a magnetic stirrer at a stirring speed of 800 rpm to 1200 rpm.

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