Antioxidant natural composition and health food thereof

Abstract

The invention is suitable for the technical field of health-care food, and provides an antioxidant natural composition and health-care food thereof.The antioxidant natural composition is prepared from the following raw materials: a coral grape bacteria extract, astaxanthin, an oregano extract, a rosemary extract, a blueberry extract and tea polyphenol.The invention further provides the health-care food. Comprising the antioxidant natural composition and auxiliary materials, and the auxiliary materials comprise modified nano zeolite powder and modified nano bacterial cellulose. According to the antioxidant natural composition provided by the invention, natural plant extracts such as the coral grape bacteria extract, the astaxanthin, the origanum vulgare extract, the rosemary extract, the blueberry extract and the tea polyphenol are combined and complemented with one another and have a synergistic effect, multiple antioxidant mechanisms are adopted, a comprehensive antioxidant system is established, and the antioxidant natural composition has a remarkable antioxidant effect.

Description

Technical Field

[0001] The invention belongs to the technical field of health food, and in particular relates to an antioxidant natural composition and a health food thereof. Background Art

[0002] There are various free radicals in the body, which are the culprits of diseases and aging. Once the body's ability to eliminate free radicals weakens, diseases will follow. Supplementing antioxidants is beneficial for the body to reduce the production of free radicals or accelerate their removal to fight the side effects of free radicals, which may delay the occurrence of sports fatigue and speed up physical recovery. The body's antioxidants are either synthesized by itself or provided by food.

[0003] The food structure in our daily diet is monotonous, and there are many varieties of health products on the market, but the antioxidant ingredients are single, the quality is uneven, and the antioxidant effect is poor. Summary of the invention

[0004] The present invention provides a natural antioxidant composition, aiming to solve the above problems.

[0005] The present invention is achieved in this way: an antioxidant natural composition comprises the following raw materials in parts by weight: 30-50 parts of Botrytis cinerea extract, 15-20 parts of astaxanthin, 20-30 parts of oregano extract, 15-25 parts of rosemary extract, 10-30 parts of blueberry extract and 5-15 parts of tea polyphenols.

[0006] Preferably, the following raw materials are included in parts by weight: 35-45 parts of Botrytis cinerea extract, 16-19 parts of astaxanthin, 22-27 parts of oregano extract, 18-23 parts of rosemary extract, 15-25 parts of blueberry extract, and 8-12 parts of tea polyphenols.

[0007] Preferably, the following raw materials are included in parts by weight: 40 parts of Botrytis cinerea extract, 17.5 parts of astaxanthin, 25 parts of oregano extract, 20 parts of rosemary extract, 20 parts of blueberry extract, and 10 parts of tea polyphenols.

[0008] Preferably, the preparation method of the grape-like coral fungus extract comprises the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain a grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 1-3:1:8-12, stirring evenly, ultrasonically treating it at a power of 100-120w for 5-10min to obtain a treated liquid, mixing the treated liquid with double distilled water, keeping it warm at 80-90°C for 60-80min, and then centrifuging it at 1200-1500r / min for 10-15min to separate a supernatant, and spray-drying the supernatant to obtain a grape-like coral fungus extract; the graphene nanosheets have an extremely narrow thickness and a high aspect ratio, can easily penetrate the cell wall, and have an extremely high wall breaking rate in combination with low-power ultrasonic treatment, which is conducive to the extraction of antioxidant components of the grape-like coral fungus, and can avoid high-power ultrasonic treatment causing water to produce a large number of free radicals that weaken the performance of the antioxidant components.

[0009] Preferably, the preparation method of the oregano extract has the following steps: extracting oregano at 60-80° C. using a multi-stage dynamic countercurrent extraction method, using 50-80% ethanol aqueous solution as solvent, using a solvent amount of 4-8 L / kg oregano, and the time of each stage of dynamic countercurrent extraction is 50-60 min. After the extraction, concentrating the obtained extract until no solvent remains to obtain an extract; performing secondary extraction on the extract using cyclohexane as solvent, after the extraction, filtering the filtrate and passing it through a polyamide adsorption column; washing the polyamide adsorption column with 4 times the volume of water, with an elution flow rate of 1-1.5 BV / h, and then desorbing it with 70-85% ethanol by volume. After the eluate is concentrated, it is completely extracted twice with n-hexane, concentrated, and dried to obtain the oregano extract.

[0010] Preferably, the preparation method of the rosemary extract has the following steps: taking rosemary, adding 0.001-0.005 times the mass of cellulase for enzymolysis, adding 15-20 times the mass of rosemary and 60-80% ethanol solution to extract rosemary enzymolysis products, concentrating the extract, centrifuging and collecting the centrifugal supernatant, subjecting the centrifugal supernatant to yeast aerobic fermentation for 1-2h at 30-35°C, wherein the amount of yeast used is 0.001-0.005 times the mass of rosemary, and then purifying through a macroporous resin, wherein the macroporous resin eluent is a 60-65% volume fraction ethanol solution with a pH of 5, and finally concentrating and drying at 60-80°C for 2-4h to obtain the rosemary extract.

[0011] Preferably, the preparation method of the blueberry extract comprises the following steps: washing and crushing the blueberries, grinding and crushing them with a colloid mill for 20-30 minutes, adding them to an ethanol solution with a concentration of 10-20 times the weight and 40-60%, adjusting the pH to 4-6, and obtaining a mixture; adding 1-2% of the weight of pectinase to the mixture, performing enzymolysis at 30-40°C for 1-2 hours to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 2-3, extracting at 20-30°C for 1-2 hours to obtain an extract; centrifuging and filtering the extract and then concentrating it, first using a rising membrane concentration equipment to perform primary concentration at a vacuum degree of 0.07-0.08Mpa and 55-65°C to obtain a primary concentrate; then performing vacuum scraper film concentration on the primary concentrate at a vacuum degree of 0.08-0.09Mpa and 60-70°C to obtain a secondary concentrate, and drying the secondary concentrate to obtain a blueberry extract.

[0012] The present invention also provides a health food, comprising the above-mentioned antioxidant natural composition and auxiliary materials, the mass ratio of the antioxidant natural composition to the auxiliary materials is 2-5:1, the auxiliary materials include modified nano zeolite powder and modified nano bacterial cellulose, the mass ratio of the two is 1-3:1, the antioxidant natural composition is adsorbed by the auxiliary materials, the antioxidant active ingredients are evenly dispersed in the auxiliary material structure, the sustained release of the active ingredients is controlled, and the shelf life of the composition is extended.

[0013] Preferably, the preparation method of the modified nano zeolite powder comprises the following steps: taking 20-50 parts by weight of natural clinoptilolite, dispersing and adding 150-200 parts by weight of hypochlorous acid solution with a concentration of 20-30%, heating in a water bath at 40-50°C and stirring for 50-60min, then adding 100-200 parts by weight of sodium hydroxide solution with a concentration of 1-2M, heating in a water bath at 60-90°C while stirring for 1-2h, filtering and rinsing with deionized water to neutrality to obtain an intermediate product, then adding 10-15 times the mass of the intermediate product of polyethylene glycol aqueous solution, the concentration of the polyethylene glycol aqueous solution is 10-20%, stirring at 50-60°C for 30-50min, filtering and drying at 60-80°C, and crushing to 60-80nm. Nanoparticles with a particle size of 0.1 mm / s are obtained to obtain modified nano zeolite powder; the trace elements in zeolite have an antioxidant effect, which can neutralize free radicals and reduce the damage of free radicals to cells, thereby enhancing the body's antioxidant capacity. Zeolite can also activate the human immune system, enhance immunity, and improve the body's resistance. By adding hypochlorous acid, heating and stirring, hypochlorous acid decomposes to produce oxygen and hydrochloric acid. The hydrochloric acid immersion corrodes and modifies the zeolite, further presenting a porous feature. The generated oxygen forms small bubbles and enters the zeolite, generating pressure to create a larger space inside the zeolite, thereby improving adsorption and being able to adsorb more antioxidant components. The zeolite is activated by hydrochloric acid and sodium hydroxide, and polyethylene glycol is added after activation to further increase the specific surface area and dispersibility of the zeolite, which has a stable antioxidant effect.

[0014] Preferably, the preparation method of the modified nano bacterial cellulose has the following steps: 15-20 parts by weight of bacterial cellulose is crushed by a wall breaking machine, and then homogenized by a high-pressure homogenizer to obtain nano bacterial cellulose, and then 0.5-1 parts by weight of vitamin C, 1-2 parts by weight of chitosan, and 60-70 parts by weight of deionized water are added, stirred at 60-80°C for 30-50 minutes, and then spray-dried to obtain modified nano bacterial cellulose; by adding vitamin C and chitosan, the antioxidant and antibacterial properties of the nano bacterial cellulose are improved.

[0015] Compared with the prior art, the embodiments of the present application have the following beneficial effects: The antioxidant natural composition provided by the present invention is a combination of natural plant extracts such as Botrytis cinerea extract, astaxanthin, oregano extract, rosemary extract, blueberry extract, tea polyphenols, etc., which complement and synergize with each other, have multiple antioxidant mechanisms, establish a comprehensive antioxidant system, and have significant antioxidant effects. DETAILED DESCRIPTION

[0016] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art to which this application belongs; the terms used in the specification of the application herein are only for the purpose of describing specific embodiments and are not intended to limit this application; the terms "including" and "having" and any variations thereof in the specification and claims of this application are intended to cover non-exclusive inclusions. The terms "first", "second", etc. in the specification and claims of this application are used to distinguish different objects, not to describe a specific order.

[0017] Reference to "embodiments" herein means that a particular feature, structure, or characteristic described in conjunction with the embodiments may be included in at least one embodiment of the present application. The appearance of the phrase in various locations in the specification does not necessarily refer to the same embodiment, nor is it an independent or alternative embodiment that is mutually exclusive with other embodiments. It is explicitly and implicitly understood by those skilled in the art that the embodiments described herein may be combined with other embodiments.

[0018] Example 1 The embodiment of the present invention provides an antioxidant natural composition, comprising the following raw materials in parts by weight: 30 parts of Botrytis cinerea extract, 15 parts of astaxanthin, 20 parts of oregano extract, 15 parts of rosemary extract, 10 parts of blueberry extract, and 5 parts of tea polyphenols.

[0019] The preparation method of the grape-like coral fungus extract comprises the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 1:1:8, stirring them evenly, and ultrasonically treating them at a power of 100w for 5min to obtain a treated liquid, mixing the treated liquid with double distilled water, keeping it warm at 80°C for 60min, and then centrifuging it at 1200r / min for 10min to separate the supernatant, and spray-drying the supernatant to obtain the grape-like coral fungus extract.

[0020] Specifically, the preparation method of the oregano extract has the following steps: extracting oregano at 60°C using a multi-stage dynamic countercurrent extraction method, using a 50% ethanol aqueous solution as a solvent, using a solvent amount of 4L / kg of oregano, and the time of each stage of dynamic countercurrent extraction being 50min. After the extraction is completed, concentrating the obtained extract until no solvent remains to obtain an extract; performing secondary extraction on the extract using cyclohexane as a solvent, after the extraction is completed, filtering the filtrate and passing it through a polyamide adsorption column; washing the polyamide adsorption column with 4 times the volume of water, with an elution flow rate of 1BV / h, and then desorbing with ethanol with a volume fraction of 70%. After the eluate is concentrated, completely extracting twice with n-hexane, concentrating, and drying to obtain the oregano extract.

[0021] In this embodiment, the preparation method of the rosemary extract has the following steps: taking rosemary, adding 0.001 times of its mass of cellulase for enzymolysis, adding 15 times of the mass of rosemary and 60% ethanol solution to extract the rosemary enzymolysis product, concentrating the extract, centrifuging and collecting the centrifugal supernatant, subjecting the centrifugal supernatant to yeast aerobic fermentation for 1 hour at 30° C., wherein the yeast dosage is 0.001 times of the mass of rosemary, and then purifying by macroporous resin, wherein the macroporous resin eluent is a 60% volume fraction ethanol solution with a pH of 5, and finally concentrating and drying at 60° C. for 2 hours to obtain the rosemary extract.

[0022] Preferably, the preparation method of the blueberry extract has the following steps: washing and crushing the blueberries, grinding and crushing them with a colloid mill for 20 minutes, adding them to an ethanol solution with a concentration of 10 times the weight and 40%, adjusting the pH to 4, and obtaining a mixture; adding 1% of the weight of pectinase to the mixture, performing enzymolysis at 30°C for 1 hour to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 2, extracting at 20°C for 1 hour to obtain an extract; centrifuging and filtering the extract and then concentrating it, first using a rising film concentration equipment to perform primary concentration at a vacuum degree of 0.07Mpa and 55°C to obtain a primary concentrated solution; then performing vacuum scraper film concentration on the primary concentrated solution at a vacuum degree of 0.08Mpa and 60°C to obtain a secondary concentrated solution, and drying the secondary concentrated solution to obtain a blueberry extract.

[0023] Example 2 The embodiment of the present invention provides an antioxidant natural composition, comprising the following raw materials in parts by weight: 35 parts of Botrytis cinerea extract, 16 parts of astaxanthin, 22 parts of oregano extract, 18 parts of rosemary extract, 15 parts of blueberry extract, and 8 parts of tea polyphenols.

[0024] The preparation method of the grape-like coral fungus extract comprises the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 1:1:8, stirring them evenly, and ultrasonically treating them at a power of 100w for 5min to obtain a treated liquid, mixing the treated liquid with double distilled water, keeping it warm at 80°C for 60min, and then centrifuging it at 1200r / min for 10min to separate the supernatant, and spray-drying the supernatant to obtain the grape-like coral fungus extract.

[0025] Specifically, the preparation method of the oregano extract has the following steps: extracting oregano at 60°C using a multi-stage dynamic countercurrent extraction method, using a 50% ethanol aqueous solution as a solvent, using a solvent amount of 4L / kg of oregano, and the time of each stage of dynamic countercurrent extraction being 50min. After the extraction is completed, concentrating the obtained extract until no solvent remains to obtain an extract; performing secondary extraction on the extract using cyclohexane as a solvent, after the extraction is completed, filtering the filtrate and passing it through a polyamide adsorption column; washing the polyamide adsorption column with 4 times the volume of water, with an elution flow rate of 1BV / h, and then desorbing with ethanol with a volume fraction of 70%. After the eluate is concentrated, completely extracting twice with n-hexane, concentrating, and drying to obtain the oregano extract.

[0026] In this embodiment, the preparation method of the rosemary extract has the following steps: taking rosemary, adding 0.001 times of its mass of cellulase for enzymolysis, adding 15 times of the mass of rosemary and 60% ethanol solution to extract the rosemary enzymolysis product, concentrating the extract, centrifuging and collecting the centrifugal supernatant, subjecting the centrifugal supernatant to yeast aerobic fermentation for 1 hour at 30° C., wherein the yeast dosage is 0.001 times of the mass of rosemary, and then purifying by macroporous resin, wherein the macroporous resin eluent is a 60% volume fraction ethanol solution with a pH of 5, and finally concentrating and drying at 60° C. for 2 hours to obtain the rosemary extract.

[0027] Preferably, the preparation method of the blueberry extract has the following steps: washing and crushing the blueberries, grinding and crushing them with a colloid mill for 20 minutes, adding them to an ethanol solution with a concentration of 10 times the weight and 40%, adjusting the pH to 4, and obtaining a mixture; adding 1% of the weight of pectinase to the mixture, performing enzymolysis at 30°C for 1 hour to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 2, extracting at 20°C for 1 hour to obtain an extract; centrifuging and filtering the extract and then concentrating it, first using a rising film concentration equipment to perform primary concentration at a vacuum degree of 0.07Mpa and 55°C to obtain a primary concentrated solution; then performing vacuum scraper film concentration on the primary concentrated solution at a vacuum degree of 0.08Mpa and 60°C to obtain a secondary concentrated solution, and drying the secondary concentrated solution to obtain a blueberry extract.

[0028] Example 3 The embodiment of the present invention provides an antioxidant natural composition, comprising the following raw materials in parts by weight: 40 parts of Botrytis cinerea extract, 17.5 parts of astaxanthin, 25 parts of oregano extract, 20 parts of rosemary extract, 20 parts of blueberry extract, and 10 parts of tea polyphenols.

[0029] The preparation method of the grape-like coral fungus extract comprises the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 2:1:10, stirring them evenly, and ultrasonically treating them at a power of 110w for 7.5min to obtain a treated liquid, mixing the treated liquid with double distilled water, keeping it warm at 85°C for 70min, and then centrifuging it at 1350r / min for 12.5min to separate the supernatant, and spray drying the supernatant to obtain the grape-like coral fungus extract.

[0030] Specifically, the preparation method of the oregano extract has the following steps: extracting oregano at 70°C using a multi-stage dynamic countercurrent extraction method, using 65% ethanol aqueous solution as a solvent, using a solvent amount of 6L / kg oregano, and the time of each stage of dynamic countercurrent extraction being 55min. After the extraction is completed, the obtained extract is concentrated until no solvent remains to obtain an extract; the extract is secondary extracted using cyclohexane as a solvent, and after the extraction is completed, the filtrate is filtered and passed through a polyamide adsorption column; the polyamide adsorption column is washed with 4 times the volume of water, the elution flow rate is 1.25BV / h, and then desorbed with ethanol with a volume fraction of 78%. After the eluate is concentrated, it is completely extracted twice with n-hexane, concentrated, and dried to obtain the oregano extract.

[0031] In this embodiment, the preparation method of the rosemary extract has the following steps: taking rosemary, adding 0.003 times of its mass of cellulase for enzymolysis, adding 18 times of the mass of rosemary and 70% ethanol solution to extract the rosemary enzymolysis product, concentrating the extract, centrifuging and collecting the centrifugal supernatant, subjecting the centrifugal supernatant to yeast aerobic fermentation for 1.5 hours at 32° C., wherein the yeast dosage is 0.003 times of the mass of rosemary, and then purifying by macroporous resin, wherein the macroporous resin eluent is a 62% volume fraction ethanol solution with a pH of 5, and finally concentrating and drying at 70° C. for 3 hours to obtain the rosemary extract.

[0032] Preferably, the preparation method of the blueberry extract has the following steps: washing and crushing the blueberries, grinding and crushing them with a colloid mill for 25 minutes, adding them to an ethanol solution with a weight of 15 times and a concentration of 50%, adjusting the pH to 5, and obtaining a mixture; adding 1.5% of the weight of pectinase to the mixture, performing enzymolysis at 35°C for 1.5 hours to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 2, extracting at 25°C for 1.5 hours to obtain an extract; centrifuging and filtering the extract and then concentrating it, first using a rising film concentration equipment to perform primary concentration at a vacuum degree of 0.075Mpa and 60°C to obtain a primary concentrated solution; then performing vacuum scraper film concentration on the primary concentrated solution at a vacuum degree of 0.085Mpa and 65°C to obtain a secondary concentrated solution, and drying the secondary concentrated solution to obtain a blueberry extract.

[0033] Example 4 The embodiment of the present invention provides an antioxidant natural composition, comprising the following raw materials in parts by weight: 45 parts of Botrytis cinerea extract, 19 parts of astaxanthin, 27 parts of oregano extract, 23 parts of rosemary extract, 25 parts of blueberry extract, and 12 parts of tea polyphenols.

[0034] The preparation method of the grape-like coral fungus extract comprises the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 3:1:12, stirring them evenly, and ultrasonically treating them at a power of 120w for 10min to obtain a treated liquid, mixing the treated liquid with double distilled water, keeping it warm at 90°C for 80min, and then centrifuging at 1500r / min for 15min to separate the supernatant, and spray-drying the supernatant to obtain the grape-like coral fungus extract.

[0035] Specifically, the preparation method of the oregano extract has the following steps: extracting oregano at 80°C using a multi-stage dynamic countercurrent extraction method, using 80% ethanol aqueous solution as a solvent, using a solvent amount of 8L / kg of oregano, and the time of each stage of dynamic countercurrent extraction being 60min. After the extraction is completed, concentrating the obtained extract until no solvent remains to obtain an extract; performing secondary extraction on the extract using cyclohexane as a solvent, after the extraction is completed, filtering the filtrate and passing it through a polyamide adsorption column; washing the polyamide adsorption column with 4 times the volume of water, with an elution flow rate of 1.5BV / h, and then desorbing with ethanol with a volume fraction of 85%. After the eluate is concentrated, completely extracting twice with n-hexane, concentrating, and drying to obtain the oregano extract.

[0036] In this embodiment, the preparation method of the rosemary extract has the following steps: taking rosemary, adding 0.005 times the mass of cellulase for enzymolysis, adding 20 times the mass of rosemary and 80% ethanol solution to extract the rosemary enzymolysis product, concentrating the extract, centrifuging and collecting the centrifugal supernatant, subjecting the centrifugal supernatant to yeast aerobic fermentation for 2h at 35°C, wherein the yeast dosage is 0.005 times the mass of rosemary, and then purifying by macroporous resin, wherein the macroporous resin eluent is a 65% volume fraction ethanol solution with a pH of 5, and finally concentrating and drying at 80°C for 4h to obtain the rosemary extract.

[0037] Preferably, the preparation method of the blueberry extract has the following steps: washing and crushing the blueberries, grinding and crushing them with a colloid mill for 30 minutes, adding them to an ethanol solution of 20 times the weight and a concentration of 60%, adjusting the pH to 6, and obtaining a mixture; adding 2% of the weight of pectinase to the mixture, performing enzymolysis at 40°C for 2 hours to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 3, extracting at 30°C for 2 hours to obtain an extract; centrifuging and filtering the extract and then concentrating it, first using a rising film concentration equipment to perform primary concentration at a vacuum degree of 0.08Mpa and 65°C to obtain a primary concentrated solution; then performing vacuum scraper film concentration on the primary concentrated solution at a vacuum degree of 0.09Mpa and 70°C to obtain a secondary concentrated solution, and drying the secondary concentrated solution to obtain a blueberry extract.

[0038] Example 5 The embodiment of the present invention provides an antioxidant natural composition, comprising the following raw materials in parts by weight: 50 parts of Botrytis cinerea extract, 20 parts of astaxanthin, 30 parts of oregano extract, 25 parts of rosemary extract, 30 parts of blueberry extract, and 15 parts of tea polyphenols.

[0039] The preparation method of the grape-like coral fungus extract comprises the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 3:1:12, stirring them evenly, and ultrasonically treating them at a power of 120w for 10min to obtain a treated liquid, mixing the treated liquid with double distilled water, keeping it warm at 90°C for 80min, and then centrifuging at 1500r / min for 15min to separate the supernatant, and spray-drying the supernatant to obtain the grape-like coral fungus extract.

[0040] Specifically, the preparation method of the oregano extract has the following steps: extracting oregano at 80°C using a multi-stage dynamic countercurrent extraction method, using 80% ethanol aqueous solution as a solvent, using a solvent amount of 8L / kg of oregano, and the time of each stage of dynamic countercurrent extraction being 60min. After the extraction is completed, concentrating the obtained extract until no solvent remains to obtain an extract; performing secondary extraction on the extract using cyclohexane as a solvent, after the extraction is completed, filtering the filtrate and passing it through a polyamide adsorption column; washing the polyamide adsorption column with 4 times the volume of water, with an elution flow rate of 1.5BV / h, and then desorbing with ethanol with a volume fraction of 85%. After the eluate is concentrated, completely extracting twice with n-hexane, concentrating, and drying to obtain the oregano extract.

[0041] In this embodiment, the preparation method of the rosemary extract has the following steps: taking rosemary, adding 0.005 times the mass of cellulase for enzymolysis, adding 20 times the mass of rosemary and 80% ethanol solution to extract the rosemary enzymolysis product, concentrating the extract, centrifuging and collecting the centrifugal supernatant, subjecting the centrifugal supernatant to yeast aerobic fermentation for 2h at 35°C, wherein the yeast dosage is 0.005 times the mass of rosemary, and then purifying by macroporous resin, wherein the macroporous resin eluent is a 65% volume fraction ethanol solution with a pH of 5, and finally concentrating and drying at 80°C for 4h to obtain the rosemary extract.

[0042] Preferably, the preparation method of the blueberry extract has the following steps: washing and crushing the blueberries, grinding and crushing them with a colloid mill for 30 minutes, adding them to an ethanol solution of 20 times the weight and a concentration of 60%, adjusting the pH to 6, and obtaining a mixture; adding 2% of the weight of pectinase to the mixture, performing enzymolysis at 40°C for 2 hours to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 3, extracting at 30°C for 2 hours to obtain an extract; centrifuging and filtering the extract and then concentrating it, first using a rising film concentration equipment to perform primary concentration at a vacuum degree of 0.08Mpa and 65°C to obtain a primary concentrated solution; then performing vacuum scraper film concentration on the primary concentrated solution at a vacuum degree of 0.09Mpa and 70°C to obtain a secondary concentrated solution, and drying the secondary concentrated solution to obtain a blueberry extract.

[0043] Example 6 An embodiment of the present invention provides a health food, comprising the above-mentioned natural antioxidant composition and auxiliary materials, preferably comprising the natural antioxidant composition of Example 3, wherein the mass ratio of the natural antioxidant composition to the auxiliary materials is 2:1, and the auxiliary materials include modified nano zeolite powder and modified nano bacterial cellulose, and the mass ratio of the two is 1:1.

[0044] In this embodiment, the preparation method of the modified nano zeolite powder is as follows: 20 parts by weight of natural clinoptilolite is dispersed and added with 150 parts by weight of a 20% hypochlorous acid solution, heated in a water bath at 40°C and stirred for 50 minutes, and then 100 parts by weight of a 1M sodium hydroxide solution is added, and heated in a water bath at 60°C for 1 hour while stirring, and filtered and rinsed with deionized water to neutrality to obtain an intermediate product, and then 10 times the mass of the intermediate product is added. The concentration of the polyethylene glycol aqueous solution is 10%, stirred at 50°C for 30 minutes, filtered and dried at 60°C, and crushed to nanoparticles with a particle size of 60 nm to obtain modified nano zeolite powder.

[0045] Furthermore, the preparation method of the modified nano bacterial cellulose has the following steps: 15 parts by weight of bacterial cellulose is crushed by a wall breaking machine, and then homogenized by a high-pressure homogenizer to obtain nano bacterial cellulose, and then 0.5 parts by weight of vitamin C, 1 part by weight of chitosan, and 60 parts by weight of deionized water are added, stirred at 60°C for 30 minutes, and then spray-dried to obtain modified nano bacterial cellulose.

[0046] Example 7 An embodiment of the present invention provides a health food, comprising the above-mentioned natural antioxidant composition and auxiliary materials, preferably comprising the natural antioxidant composition of Example 3, wherein the mass ratio of the natural antioxidant composition to the auxiliary materials is 3.5:1, and the auxiliary materials include modified nano zeolite powder and modified nano bacterial cellulose, and the mass ratio of the two is 2:1.

[0047] In this embodiment, the preparation method of the modified nano zeolite powder is as follows: 35 parts by weight of natural clinoptilolite is dispersed and added with 175 parts by weight of a 25% hypochlorous acid solution, heated in a water bath at 45°C and stirred for 55 minutes, and then 150 parts by weight of a 1.5M sodium hydroxide solution is added, and heated in a water bath at 75°C while stirring for 1.5 hours, filtered and rinsed with deionized water to neutrality to obtain an intermediate product, and then 12.5 times the mass of the intermediate product is added. The concentration of the polyethylene glycol aqueous solution is 15%, stirred at 55°C for 40 minutes, filtered and dried at 70°C, and crushed to nanoparticles with a particle size of 70 nm to obtain modified nano zeolite powder.

[0048] Furthermore, the preparation method of the modified nano bacterial cellulose has the following steps: 17.5 parts by weight of bacterial cellulose is crushed by a wall breaking machine, and then homogenized by a high-pressure homogenizer to obtain nano bacterial cellulose, and then 0.75 parts by weight of vitamin C, 1.5 parts by weight of chitosan, and 65 parts by weight of deionized water are added, stirred at 70°C for 40 minutes, and then spray-dried to obtain modified nano bacterial cellulose.

[0049] Example 8 An embodiment of the present invention provides a health food, comprising the above-mentioned natural antioxidant composition and auxiliary materials, preferably comprising the natural antioxidant composition of Example 3, wherein the mass ratio of the natural antioxidant composition to the auxiliary materials is 5:1, and the auxiliary materials include modified nano zeolite powder and modified nano bacterial cellulose, and the mass ratio of the two is 3:1.

[0050] In this embodiment, the preparation method of the modified nano zeolite powder is as follows: 50 parts by weight of natural clinoptilolite is dispersed and added with 200 parts by weight of a 30% hypochlorous acid solution, heated in a water bath at 50°C and stirred for 60 minutes, then 200 parts by weight of a 2M sodium hydroxide solution is added, and heated in a water bath at 90°C for 2 hours while stirring, filtered and rinsed with deionized water until neutral to obtain an intermediate product, and then 15 times the mass of the intermediate product is added. The concentration of the polyethylene glycol aqueous solution is 20%, stirred at 60°C for 50 minutes, filtered and dried at 80°C, and crushed to nanoparticles with a particle size of 80 nm to obtain modified nano zeolite powder.

[0051] Furthermore, the preparation method of the modified nano bacterial cellulose has the following steps: 20 parts by weight of bacterial cellulose are crushed by a wall breaking machine, and then homogenized by a high-pressure homogenizer to obtain nano bacterial cellulose, and then 0.1 parts by weight of vitamin C, 2 parts by weight of chitosan, and 70 parts by weight of deionized water are added, stirred at 80°C for 50 minutes, and then spray-dried to obtain modified nano bacterial cellulose.

[0052] Comparative Example 1: Compared with Example 3, the extract of Botrytis cinerea was replaced with an equal amount of distilled water.

[0053] Comparative Example 2: Compared with Example 3, the oregano extract was replaced with an equal amount of distilled water.

[0054] Comparative Example 3: Compared with Example 3, astaxanthin was replaced with an equal amount of distilled water.

[0055] Comparative Example 4: Compared with Example 3, the Botrytis cinerea extract, oregano extract and astaxanthin were all replaced with an equal amount of distilled water.

[0056] Comparative Example 5: Compared with Example 7, the modified nano zeolite powder is replaced by ordinary nano zeolite powder.

[0057] Comparative Example 6: Compared with Example 7, the modified nano bacterial cellulose is replaced by ordinary nano bacterial cellulose.

[0058] Comparative Example 7: Compared with Example 7, the modified nano zeolite powder is replaced by ordinary nano zeolite powder and the modified nano bacterial fiber is replaced by ordinary nano bacterial cellulose.

[0059] Antioxidant performance test Determination of DPPH free radical scavenging rate Test method: Take 2.0mL of 120μmol / L DPPH free radical solution and add it to 20μL of the sample to be tested. Replace the sample with the same dose of double distilled water and add the same volume of DPPH free radical solution as the control (A0). Replace the DPPH free radical solution with 2.0mL of 50% ethanol to adjust to zero. After placing it at 25℃ for 20min, measure the absorbance of the DPPH free radical mixed solution at 520nm (sample A). The scavenging ability is expressed as the scavenging rate. Repeat 3 times and calculate according to the formula: DPPH free radical scavenging rate % = (A0-sample A) × 100 / A0. The test results are shown in Table 1 below: Table 1 DPPH free radical scavenging rate

[0060] It can be seen from the test results of Examples 1-5 and Comparative Examples 1-4 that the antioxidant natural composition prepared by the present invention has good antioxidant performance, and there is a synergistic effect between the botrytis cinerea extract, the oregano extract and astaxanthin, which can further improve the antioxidant performance.

[0061] It can be seen from the test results of Examples 6-8 and Comparative Examples 5-7 that the health food prepared by the present invention has good antioxidant properties, and the antioxidant properties can be further improved by adding modified nano zeolite powder and modified nano bacterial cellulose.

[0062] It should be noted that, for the above embodiments, for the sake of simplicity, they are all described as a series of action combinations, but those skilled in the art should know that the present invention is not limited by the described order of actions, because according to the present invention, some steps may be performed in other orders or simultaneously. Secondly, those skilled in the art should also know that the embodiments described in the specification are all preferred embodiments, and the actions and modules involved are not necessarily required by the present invention.

[0063] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the scope of protection of the invention. Obviously, the described embodiments are only some embodiments of the present invention, rather than all embodiments. Based on these embodiments, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in this field can still combine, add, delete or make other adjustments to the features in the various embodiments of the present invention according to the circumstances without conflict, without making creative work, so as to obtain different other technical solutions that do not deviate from the concept of the present invention in essence, and these technical solutions also belong to the scope of protection of the present invention.

Claims

1. An antioxidant natural composition, characterized in that: The invention comprises the following raw materials in parts by weight: 30-50 parts of Botrytis cinerea extract, 15-20 parts of astaxanthin, 20-30 parts of oregano extract, 15-25 parts of rosemary extract, 10-30 parts of blueberry extract and 5-15 parts of tea polyphenols.

2. The antioxidant natural composition according to claim 1, characterized in that The invention comprises the following raw materials in parts by weight: 35-45 parts of Botrytis cinerea extract, 16-19 parts of astaxanthin, 22-27 parts of oregano extract, 18-23 parts of rosemary extract, 15-25 parts of blueberry extract and 8-12 parts of tea polyphenols.

3. The antioxidant natural composition according to claim 2, characterized in that The invention comprises the following raw materials in parts by weight: 40 parts of Botrytis cinerea extract, 17.5 parts of astaxanthin, 25 parts of oregano extract, 20 parts of rosemary extract, 20 parts of blueberry extract and 10 parts of tea polyphenols.

4. The antioxidant natural composition according to claim 1, characterized in that The preparation method of the grape-like coral fungus extract has the following steps: cleaning the grape-like coral fungus, drying it to a constant weight, crushing and sieving it to obtain grape-like coral fungus powder; mixing the grape-like coral fungus powder, graphene nanosheets and deionized water in a mass ratio of 1-3:1:8-12, stirring them evenly, performing ultrasonic treatment at a power of 100-120w for 5-10min to obtain a treatment liquid, mixing the treatment liquid with double distilled water, keeping warm at 80-90°C for 60-80min, and then centrifuging at 1200-1500r / min for 10-15min to separate a supernatant, and spray-drying the supernatant to obtain the grape-like coral fungus extract.

5. The antioxidant natural composition according to claim 1, characterized in that The preparation method of the oregano extract has the following steps: extracting oregano at 60-80°C by a multi-stage dynamic countercurrent extraction method, using 50-80% ethanol aqueous solution as solvent, using 4-8L / kg of oregano as solvent, and the time of each stage of dynamic countercurrent extraction is 50-60min. After the extraction, the obtained extract is concentrated until no solvent remains to obtain an extract; the extract is secondary extracted by using cyclohexane as solvent, after the extraction, the filtrate is filtered and passed through a polyamide adsorption column; the polyamide adsorption column is washed with 4 times the volume of water, the elution flow rate is 1-1.5BV / h, and then desorbed with 70-85% ethanol by volume. After the eluate is concentrated, it is completely extracted twice with normal hexane, concentrated, and dried to obtain the oregano extract.

6. The antioxidant natural composition according to claim 1, characterized in that The preparation method of the rosemary extract comprises the following steps: taking rosemary, adding 0.001-0.005 times of the mass of cellulase for enzymolysis, adding 15-20 times of the mass of rosemary and 60-80% of ethanol solution to extract rosemary enzymolysis product, concentrating the extract, collecting centrifugal supernatant by centrifugation, aerobic fermenting the centrifugal supernatant with yeast at 30-35 DEG C for 1-2h, wherein the amount of yeast used is 0.001-0.005 times of the mass of rosemary, purifying by macroporous resin, wherein the eluent of the macroporous resin is 60-65% volume fraction ethanol solution with pH=5, and finally concentrating and drying at 60-80 DEG C for 2-4h to obtain the rosemary extract.

7. The antioxidant natural composition according to claim 1, characterized in that The preparation method of the blueberry extract comprises the following steps: washing and crushing the blueberries, grinding and crushing the blueberries with a colloid mill for 20-30 minutes, adding the blueberries to an ethanol solution with a weight of 10-20 times and a concentration of 40-60%, adjusting the pH to 4-6, and obtaining a mixture; adding pectinase with a weight of 1-2% of the mixture, performing enzymolysis at 30-40° C. for 1-2 hours to obtain an enzymolysis solution; adjusting the pH of the enzymolysis solution to 2-3, and extracting at 20-30° C. for 1-2 hours to obtain an extract; centrifuging and filtering the extract and then concentrating the extract, firstly performing primary concentration with a rising film concentration device at a vacuum degree of 0.07-0.08Mpa and 55-65° C. to obtain a primary concentrated solution; then performing vacuum scraper film concentration on the primary concentrated solution at a vacuum degree of 0.08-0.09Mpa and 60-70° C. to obtain a secondary concentrated solution, and drying the secondary concentrated solution to obtain the blueberry extract.

8. A health food, characterized in that: It comprises the antioxidant natural composition and auxiliary materials as described in any one of claims 1 to 7, wherein the mass ratio of the antioxidant natural composition to the auxiliary materials is 2-5:1, and the auxiliary materials comprise modified nano zeolite powder and modified nano bacterial cellulose, and the mass ratio of the two is 1-3:

1.

9. The health food according to claim 8, characterized in that The preparation method of the modified nano zeolite powder has the following steps: taking 20-50 parts by weight of natural clinoptilolite, dispersing and adding 150-200 parts by weight of hypochlorous acid solution with a concentration of 20-30%, heating in a water bath at 40-50° C. and stirring for 50-60 minutes, then adding 100-200 parts by weight of sodium hydroxide solution with a concentration of 1-2M, heating in a water bath at 60-90° C. while stirring for 1-2 hours, filtering and rinsing with deionized water to neutrality to obtain an intermediate product, then adding a polyethylene glycol aqueous solution 10-15 times the mass of the intermediate product, the concentration of the polyethylene glycol aqueous solution is 10-20%, stirring at 50-60° C. for 30-50 minutes, filtering and drying at 60-80° C., and crushing to nanoparticles with a particle size of 60-80 nm to obtain the modified nano zeolite powder.

10. The health food according to claim 8, characterized in that The preparation method of the modified nano bacterial cellulose has the following steps: 15-20 parts by weight of bacterial cellulose is crushed by a wall breaking machine, and then homogenized by a high-pressure homogenizer to obtain nano bacterial cellulose, and then 0.5-1 parts by weight of vitamin C, 1-2 parts by weight of chitosan, and 60-70 parts by weight of deionized water are added, stirred at 60-80° C. for 30-50 minutes, and then spray-dried to obtain the modified nano bacterial cellulose.

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