Composition containing polydeoxyribonucleotide and sodium hyaluronate and application thereof

Abstract

The invention provides a composition containing polydeoxyribonucleotide (PDRN) and sodium hyaluronate and application of the composition, and relates to the technical field of cosmetics and medical beauty. The composition is prepared from polydeoxyribonucleotide, sodium hyaluronate, L-carnosine, glycine, alanine, proline, vitamin B2, ergothioneine, Ectoine, a sophora flower bud extract, an aloe extract, butanediol and 1, 2-pentanediol. The composition is high in stability and has whitening and anti-aging effects.

Description

Technical Field

[0001] The present invention belongs to the field of cosmetics and medical aesthetics, and specifically relates to a composition containing polydeoxyribonucleotide and sodium hyaluronate and an application thereof. Background Art

[0002] Skin is the largest organ in the human body. Not only does it cover our entire body, it also plays multiple roles, protecting us from the external environment while also reflecting our health and age. The challenges facing the skin are multifaceted.

[0003] For example: long-term exposure to sunlight, ultraviolet rays can damage skin cells, leading to sunburn, pigmentation, and skin aging. Pollutants in the air, such as smoke, dust, and heavy metals, can adhere to the surface of the skin, causing inflammatory reactions and accelerating skin aging.

[0004] At the same time, with age, the number of facial skin fibroblasts decreases, the secretion of collagen and elastin decreases, the skin elasticity deteriorates, and wrinkles appear. Working overtime, staying up late at night, long hours on the computer, smoking, lack of sleep, staying in air-conditioned rooms for a long time, etc. can also have an impact on the skin, causing the skin to become loose, rough, dull, etc.

[0005] Due to these challenges, the skin requires comprehensive care and protection. Whitening products can help reduce pigmentation and brighten skin tone, while anti-aging products can enhance skin elasticity and firmness and reduce wrinkles and fine lines. The development of skin care products with both whitening and anti-aging effects is of great significance to meeting consumer needs, improving product effectiveness and safety, and promoting innovation and sustainable development of the cosmetics industry. Summary of the invention

[0006] In order to solve the above problems, the present invention provides a composition containing polydeoxyribonucleotide and sodium hyaluronate and its application.

[0007] In one aspect, the present invention provides a composition comprising polydeoxyribonucleotide and sodium hyaluronate.

[0008] Specifically, the composition contains: PDRN, sodium hyaluronate, L-carnosine, water-soluble amino acids, water-soluble vitamins, ergothioneine, ectoine, Sophora japonica extract, aloe extract and moisturizer.

[0009] Preferably, the solvent of the composition is water for injection.

[0010] More specifically, the water-soluble amino acids include: at least one of glycine, proline, hydroxyproline, lysine hydrochloride, isoleucine, leucine, serine, alanine, aspartic acid, tyrosine, glutamic acid, phenylalanine, arginine hydrochloride, valine, threonine, histidine hydrochloride, tryptophan, methionine, cystine and / or cysteine.

[0011] In certain specific embodiments of the present invention, the water-soluble amino acids include: glycine, alanine and proline.

[0012] More specifically, the water-soluble vitamins include at least one of vitamin B1, vitamin B2, niacinamide, vitamin B6, vitamin B12, folic acid and / or vitamin C.

[0013] In certain specific embodiments of the present invention, the water-soluble vitamin is vitamin B2.

[0014] More specifically, the moisturizer includes at least one of polydimethylsiloxane, saccharide isomers, citric acid, sodium citrate, 1,2-hexanediol, butylene glycol, p-hydroxyacetophenone, 1,2-pentanediol, hydroxyphenylpropionamide benzoic acid, betaine, sodium hyaluronate and / or glyceryl polyether-26.

[0015] In certain specific embodiments of the present invention, the moisturizing agent includes: butylene glycol and 1,2-pentanediol.

[0016] Specifically, the composition includes, by mass, 0.1-2wt% of PDRN, 0.1-2wt% of sodium hyaluronate, 0.01-1wt% of L-carnosine, 0.001-0.1wt% of glycine, 0.001-0.1wt% of alanine, 0.001-0.1wt% of proline, 0.001-0.1wt% of vitamins, 0.1-2wt% of ergothioneine, 0.1-2wt% of ectoine, 1-15wt% of sophora japonica seed extract, 1-15wt% of aloe vera extract, 0.1-2.5wt% of butanediol and 0.1-2.5wt% of 1,2-pentanediol, and the balance is water for injection.

[0017] Further specifically, the composition includes, by mass, 0.3-1.5wt% of PDRN, 0.3-1.5wt% of sodium hyaluronate, 0.1-0.5wt% of L-carnosine, 0.005-0.05wt% of glycine, 0.005-0.05wt% of alanine, 0.005-0.05wt% of proline, 0.005-0.05wt% of vitamins, 0.3-1wt% of ergothioneine, 0.5-1.5wt% of ectoine, 3-8wt% of sophora japonica seed extract, 3-8wt% of aloe vera extract, 0.5-1.5wt% of butanediol and 0.5-1.5wt% of 1,2-pentanediol, and the balance is water for injection.

[0018] Preferably, the composition comprises, by mass, 0.3-0.5wt% of PDRN, 0.3-0.5wt% of sodium hyaluronate, 0.1-0.2wt% of L-carnosine, 0.005-0.01wt% of glycine, 0.005-0.01wt% of alanine, 0.005-0.01wt% of proline, 0.005-0.01wt% of vitamin B2, 0.3-0.5wt% of ergothioneine, 0.5-1wt% of ectoine, 3-5wt% of sophora japonica seed extract, 3-5wt% of aloe extract, 0.5-1wt% of butanediol and 0.5-1wt% of 1,2-pentanediol, and the balance is water for injection.

[0019] Preferably, the composition comprises, by mass: 0.5-1.5wt% of PDRN, 0.5-1.5wt% of sodium hyaluronate, 0.2-0.5wt% of L-carnosine, 0.01-0.05wt% of glycine, 0.01-0.05wt% of alanine, 0.01-0.05wt% of proline, 0.01-0.05wt% of vitamin B2, 0.5-1wt% of ergothioneine, 1-1.5wt% of ectoine, 5-8wt% of sophora japonica seed extract, 5-8wt% of aloe vera extract, 1-1.5wt% of butanediol and 1-1.5wt% of 1,2-pentanediol, and the balance is water for injection.

[0020] More specifically, the preparation method of the Sophora japonica extract is:

[0021] (1) crushing the sophora japonica buds and sieving them to obtain sophora japonica bud fine powder with a particle size of less than 250 μm;

[0022] (2) taking Sophora japonica seed powder, adding 50-75% ethanol, and ultrasonically extracting;

[0023] (3) Repeat the extraction twice and combine the extracts.

[0024] Preferably, in step (1), the sieving is through a 60-mesh sieve.

[0025] Preferably, in step (2), the ratio of the fine powder of Sophora japonica seeds to ethanol is: 0.3-1 g: 25-35 mL.

[0026] Preferably, in step (2), the ultrasonic extraction conditions are: 50-75°C, 25-35min.

[0027] More specifically, the preparation method of the aloe extract is:

[0028] (1) Wash, dry, peel and dice the aloe vera;

[0029] (2) Put it in hot water at 85-100℃ to kill the green leaves;

[0030] (3) Hot air drying until the moisture content is less than 10%;

[0031] (4) crushing the aloe vera and sieving to obtain aloe vera powder with a particle size of less than 250 μm;

[0032] (5) Add 50-75% ethanol to aloe vera powder and extract by ultrasonic;

[0033] (6) Repeat the extraction twice and combine the extracts.

[0034] Preferably, in step (5), the ratio of the aloe vera powder to the ethanol is: 0.3-1 g: 25-35 mL.

[0035] Preferably, in step (5), the ultrasonic extraction conditions are: 25-35°C, 25-35min.

[0036] In another aspect, the present invention provides use of the above composition in preparing whitening and / or anti-aging products.

[0037] Compared with the prior art, the present invention has the following advantages:

[0038] The composition of the present invention has more excellent stability and has significant whitening and anti-aging effects. DETAILED DESCRIPTION

[0039] The present invention will be further described in detail below in conjunction with specific examples. The following examples are not intended to limit the present invention, but are only intended to illustrate the present invention. The experimental methods used in the following examples are generally conventional, unless otherwise specified, and the materials, reagents, etc. used in the following examples are commercially available, unless otherwise specified.

[0040] In the following examples and comparative examples, the preparation method of PDRN is the same as Example 1 of CN118546922A; hyaluronic acid and carnosine are purchased from Runhui Biotechnology (Weihai) Co., Ltd.

[0041] Example 1

[0042] Calculated by mass, it includes: PDRN 0.5wt%, sodium hyaluronate 0.5wt%, L-carnosine 0.2wt%, glycine 0.01wt%, alanine 0.01wt%, proline 0.01wt%, vitamin B2 0.01wt%, ergothioneine 0.5wt%, ectoine 1wt%, sophora japonica extract 5wt%, aloe extract 5wt%, butylene glycol 1wt%, and 1,2-pentanediol 1wt%.

[0043] The solvent is water for injection.

[0044] Preparation method of Sophora japonica extract:

[0045] After the sophora japonica seeds were crushed, they were passed through a 60-mesh sieve to obtain sophora japonica seeds powder with a particle size of less than 250 μm. 0.5 g of sophora japonica seeds powder was placed in a 50 mL volumetric flask, 15 mL of 70% ethanol was added, and ultrasonic extraction was performed at 65°C for 30 min. After ultrasonication, the mixture was filtered, and the extraction was repeated twice, and the extracts were combined.

[0046] Aloe vera extract preparation method:

[0047] Select aloe vera with no disease or spots on the surface, wash, dry and peel, dice the peeled aloe vera, put it in 95℃ hot water for 10 seconds to kill the green; dry it with hot air at 60℃ until the final water content of the aloe vera is less than 10%; crush the dried aloe vera and pass it through a 60-mesh sieve to obtain aloe vera powder with a particle size of less than 250μm. Take 0.5g of aloe vera powder and put it in a 50mL volumetric flask, add 15mL of 50% ethanol, extract it by ultrasonic at 30℃ for 30min, filter it after ultrasonic, repeat the extraction twice, and combine the extracts.

[0048] Example 2

[0049] Calculated by mass, it includes: 1.5wt% PDRN, 1.5wt% sodium hyaluronate, 0.5wt% L-carnosine, 0.05wt% glycine, 0.05wt% alanine, 0.05wt% proline, 0.05wt% vitamin B2, 1wt% ergothioneine, 1.5wt% ectoine, 8wt% sophora japonica extract, 8wt% aloe extract, 1.5wt% butanediol, and 1.5wt% 1,2-pentanediol.

[0050] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0051] The solvent is water for injection.

[0052] Example 3

[0053] Calculated by mass, it includes: PDRN 0.3wt%, sodium hyaluronate 0.3wt%, L-carnosine 0.1wt%, glycine 0.005wt%, alanine 0.005wt%, proline 0.005wt%, vitamin B2 0.005wt%, ergothioneine 0.3wt%, ectoine 0.5wt%, sophora japonica seed extract 3wt%, aloe vera extract 3wt%, butylene glycol 0.5wt%, and 1,2-pentanediol 0.5wt%.

[0054] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0055] The solvent is water for injection.

[0056] Comparative Example 1

[0057] Calculated by mass, it includes: 1wt% PDRN, 0.2wt% L-carnosine, 0.01wt% glycine, 0.01wt% alanine, 0.01wt% proline, 0.01wt% vitamin B2, 0.5wt% ergothioneine, 1wt% ectoine, 5wt% sophora japonica extract, 5wt% aloe extract, 1wt% butanediol, and 1wt% 1,2-pentanediol.

[0058] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0059] The solvent is water for injection.

[0060] Comparative Example 2

[0061] Calculated by mass, it includes: 1wt% sodium hyaluronate, 0.2wt% L-carnosine, 0.01wt% glycine, 0.01wt% alanine, 0.01wt% proline, 0.01wt% vitamin B2, 0.5wt% ergothioneine, 1wt% ectoine, 5wt% sophora japonica extract, 5wt% aloe extract, 1wt% butanediol, and 1wt% 1,2-pentanediol.

[0062] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0063] The solvent is water for injection.

[0064] Comparative Example 3

[0065] Calculated by mass, it includes: 0.5wt% PDRN, 0.5wt% sodium hyaluronate, 0.2wt% L-carnosine, 0.01wt% glycine, 0.01wt% alanine, 0.01wt% proline, 0.01wt% vitamin B2, 1.5wt% ergothioneine, 5wt% sophora japonica extract, 5wt% aloe extract, 1wt% butanediol, and 1wt% 1,2-pentanediol.

[0066] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0067] The solvent is water for injection.

[0068] Comparative Example 4

[0069] Calculated by mass, it includes: 0.5wt% PDRN, 0.5wt% sodium hyaluronate, 0.2wt% L-carnosine, 0.01wt% glycine, 0.01wt% alanine, 0.01wt% proline, 0.01wt% vitamin B2, 0.5wt% ergothioneine, 1wt% ectoine, 5wt% sophora japonica extract, 5wt% aloe extract, and 2wt% butanediol.

[0070] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0071] The solvent is water for injection.

[0072] Comparative Example 5

[0073] Calculated by mass, it includes: PDRN 0.5wt%, sodium hyaluronate 0.5wt%, L-carnosine 0.2wt%, glycine 0.01wt%, alanine 0.01wt%, proline 0.01wt%, vitamin B2 0.01wt%, ergothioneine 0.5wt%, ectoine 1wt%, sophora japonica extract 10wt%, butanediol 1wt%, and 1,2-pentanediol 1wt%.

[0074] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0075] The solvent is water for injection.

[0076] Comparative Example 6

[0077] Calculated by mass, it includes: PDRN 0.5wt%, sodium hyaluronate 0.5wt%, L-carnosine 0.2wt%, glycine 0.01wt%, alanine 0.01wt%, proline 0.01wt%, vitamin B2 0.01wt%, ergothioneine 0.5wt%, ectoine 1wt%, aloe extract 10wt%, butanediol 1wt%, and 1,2-pentanediol 1wt%.

[0078] The preparation methods of Sophora japonica extract and Aloe vera extract are the same as those in Example 1.

[0079] The solvent is water for injection.

[0080] Experimental Example 1: Stability Evaluation

[0081] Detection method: The compositions prepared from the corresponding parts of the embodiments in Examples 1-3 and the corresponding parts of the comparative examples in Comparative Examples 1-6 were placed in 100 mL sample bottles, respectively, and stored at 4°C, 25°C, 45°C and in sunlight, and the color change, taste change, and dryness and hygroscopicity of the samples were observed in a cycle of one week, and the observation was continued for 4 cycles. The experimental results are shown in Table 1.

[0082] Table 1

[0083]

[0084]

[0085] Experimental Example 2 Evaluation of Whitening Effect

[0086] Whitening effect verification

[0087] Test product: the composition obtained from Examples 1-3 and Comparative Examples 1-6 of the present invention.

[0088] Subjects: aged between 20 and 60 years old, healthy, without skin diseases, not pregnant or breastfeeding, use the Skin-Colorimeter CL400 probe and Mexameter MX18 probe of MPA580 skin elasticity tester to detect and analyze the changes in skin color, skin melanin and hemoglobin, and group them according to ITA and MI values. The difference between each group and the average ITA and MI of all subjects should not exceed 5%. Each group has 10 people

[0089] Test method: Each test group takes the corresponding composition solution and applies the sample to the face twice a day. No other products or drugs that may affect the skin condition should be used during the test. Use it continuously for 1 month, and observe whether the subjects have allergic reactions during use.

[0090] One month later, the Skin-Colorimeter CL400 probe and Mexameter MX18 probe of the MPA580 skin elasticity tester were used to detect and analyze the changes in skin color, skin melanin and hemoglobin. The results are shown in Table 2.

[0091] Table 2

[0092] Components ITA° Improvement Rate Melanin index (MI value) reduction rate Example 1 12.08％ 20.52％ Example 2 11.96％ 20.38％ Example 3 10.77％ 18.96％ Comparative Example 1 6.98％ 11.65％ Comparative Example 2 5.45％ 11.78％ Comparative Example 3 4.49％ 10.45％ Comparative Example 4 10.16％ 18.76％ Comparative Example 5 6.77％ 12.78％ Comparative Example 6 7.92％ 13.44％ .

[0093] Experimental Example 3 Evaluation of anti-aging effects

[0094] Subjects: aged between 20 and 60 years old, healthy, without skin diseases, not pregnant or breastfeeding. The skin elasticity of the subjects was tested by skin elasticity tester MPA580, the average value was calculated, and the subjects were divided into groups according to the average value. The difference between the average value of R2, R5, and R6 of each group and the average value of all subjects was no more than 5%. The test results are shown in Table 3.

[0095] Elasticity test description: Ua-skin rebound amount when there is no negative pressure; Uf-maximum skin stretch amount when there is negative pressure; Ue-positioned elastic stretch amount after constant negative pressure is applied to the skin; Uv: Uf-Ue is the viscoelastic part of the skin, or the stretch amount of the plastic part; Ur: After the negative pressure is removed, the skin will quickly return to its original state, and the stretch value of the elastic part in the recovery process; Definition of R2: R2=Ua / Uf, the ratio of the maximum stretch amount when there is negative pressure, the closer the ratio is to 1, the better the skin elasticity; Definition of R5: R5=Ur / Ue, the ratio of the elastic part in the skin recovery process to the elastic part in the negative pressure process, the closer the ratio is to 1, the better the skin elasticity; Definition of R6: R6=Uv / Ue, the ratio of the plastic part to the elastic part of the skin, the smaller the ratio is, the better the skin elasticity; Index description: The closer R2 and R5 are to 1, and the closer R6 is to 0, the better the skin elasticity.

[0096] Table 3

[0097] Components R2 SD R5 SD R6 SD - 0.55 0.035 0.56 0.034 0.35 0.032 Example 1 0.95 0.041 0.96 0.045 0.10 0.031 Example 2 0.94 0.043 0.97 0.035 0.10 0.025 Example 3 0.91 0.033 0.93 0.043 0.11 0.036 Comparative Example 1 0.71** 0.036 0.70** 0.042 0.18** 0.033 Comparative Example 2 0.69** 0.043 0.69** 0.035 0.23** 0.022 Comparative Example 3 0.67** 0.064 0.70** 0.058 0.25** 0.043 Comparative Example 4 0.85* 0.085 0.87* 0.092 0.15* 0.056 Comparative Example 5 0.72** 0.024 0.77** 0.031 0.24** 0.022 Comparative Example 6 0.71** 0.034 0.79** 0.042 0.21** 0.030

[0098] Note: relative to Example 1, * indicates P < 0.05; ** indicates P < 0.01.

[0099] Finally, it should be noted that the above content is only used to illustrate the technical solution of the present invention, rather than to limit the scope of protection of the present invention. Simple modifications or equivalent substitutions of the technical solution of the present invention by ordinary technicians in this field do not deviate from the essence and scope of the technical solution of the present invention.

Claims

1. A composition containing polydeoxyribonucleotides and sodium hyaluronate, characterized in that: The composition contains: polydeoxyribonucleotide, sodium hyaluronate, L-carnosine, water-soluble amino acids, water-soluble vitamins, ergothioneine, ectoine, sophora japonica seed extract, aloe extract and moisturizing agent; the solvent of the composition is water for injection.

2. The composition according to claim 1, characterized in that The water-soluble amino acids include at least one of glycine, proline, hydroxyproline, lysine hydrochloride, isoleucine, leucine, serine, alanine, aspartic acid, tyrosine, glutamic acid, phenylalanine, arginine hydrochloride, valine, threonine, histidine hydrochloride, tryptophan, methionine, cystine and cysteine.

3. The composition according to claim 2, characterized in that The water-soluble amino acids include glycine, alanine and proline.

4. The composition according to claim 1, characterized in that The water-soluble vitamins include at least one of vitamin B1, vitamin B2, niacinamide, vitamin B6, vitamin B12, folic acid and vitamin C.

5. The composition according to claim 4, characterized in that The water-soluble vitamin is vitamin B2.

6. The composition according to claim 1, characterized in that The moisturizing agent comprises at least one of polydimethylsiloxane, saccharide isomers, citric acid, sodium citrate, 1,2-hexanediol, butylene glycol, p-hydroxyacetophenone, 1,2-pentanediol, hydroxyphenylpropionamide benzoic acid, betaine, sodium hyaluronate and glyceryl polyether-26.

7. The composition according to claim 6, characterized in that The moisturizing agent includes butylene glycol and 1,2-pentanediol.

8. The composition according to claim 1, characterized in that The composition is prepared from the following raw materials in mass fractions: 0.1-2wt% of PDRN, 0.1-2wt% of sodium hyaluronate, 0.01-1wt% of L-carnosine, 0.001-0.1wt% of glycine, 0.001-0.1wt% of alanine, 0.001-0.1wt% of proline, 0.001-0.1wt% of vitamins, 0.1-2wt% of ergothioneine, 0.1-2wt% of ectoine, 1-15wt% of sophora japonica seed extract, 1-15wt% of aloe vera extract, 0.1-2.5wt% of butanediol and 0.1-2.5wt% of 1,2-pentanediol, and the balance is water for injection.

9. The composition according to claim 8, characterized in that The composition is prepared from the following raw materials in mass fractions: 0.3-1.5wt% of PDRN, 0.3-1.5wt% of sodium hyaluronate, 0.1-0.5wt% of L-carnosine, 0.005-0.05wt% of glycine, 0.005-0.05wt% of alanine, 0.005-0.05wt% of proline, 0.005-0.05wt% of vitamins, 0.3-1wt% of ergothioneine, 0.5-1.5wt% of ectoine, 3-8wt% of sophora japonica seed extract, 3-8wt% of aloe vera extract, 0.5-1.5wt% of butanediol and 0.5-1.5wt% of 1,2-pentanediol, and the balance is water for injection.

10. The composition according to any one of claims 1 to 9, characterized in that The preparation method of the sophora japonica extract is as follows: (1) crushing the sophora japonica seeds and sieving to obtain sophora japonica seeds fine powder with a particle size of less than 250 μm; (2) taking Sophora japonica seed powder, adding 50-75% ethanol, and ultrasonically extracting; (3) Repeat the extraction twice and combine the extracts.

11. The composition according to claim 10, characterized in that In step (2), the ratio of the fine powder of Sophora japonica seeds to ethanol is: 0.3-1 g: 25-35 mL.

12. The composition according to claim 10, characterized in that In step (2), the ultrasonic extraction conditions are: 50-75°C, 25-35min.

13. The composition according to any one of claims 1 to 9, characterized in that The preparation method of the aloe extract is: (1) Wash, dry, peel and dice the aloe vera; (2) Put it in hot water at 85-100℃ to kill the green leaves; (3) Hot air drying until the moisture content is less than 10%; (4) crushing the aloe vera and sieving to obtain aloe vera powder with a particle size of less than 250 μm; (5) Add 50-75% ethanol to aloe vera powder and extract by ultrasonic; (6) Repeat the extraction twice and combine the extracts.

14. The composition according to claim 13, characterized in that In step (5), the ratio of the aloe vera powder to the ethanol is: 0.3-1 g: 25-35 mL.

15. The composition according to claim 13, characterized in that In step (5), the ultrasonic extraction conditions are: 25-35°C, 25-35min.

16. Use of the composition according to any one of claims 1 to 15 in the preparation of whitening and / or anti-aging products.

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