Multi-component essence with whitening and moisturizing effects and preparation method thereof

Abstract

The invention relates to the technical field of cosmetics, and discloses a multi-component essence with whitening and moisturizing effects and a preparation method thereof.By designing compatibility of multiple components, the multi-component essence is prepared, all the components in freeze-dried powder A can achieve a good moisturizing effect, all the components in freeze-dried powder B can cooperate with one another, and the effect of whitening and moisturizing is achieved. A luteolin sustained-release agent uniquely prepared in the essence C is of a shell-core structure, a shell layer sequentially comprises polyglycerol fatty acid ester and Arabic gum, and the Arabic gum is a polyelectrolyte and can be adsorbed on the skin surface, so that luteolin in the core layer is slowly released on the skin surface and enters the deep layer of the skin; the activity of tyrosinase is inhibited, so that a synergistic effect is generated with the freeze-dried powder A and the freeze-dried powder B, and skin whitening is realized from multiple angles.

Description

Technical Field

[0001] The present invention relates to the technical field of cosmetics, and in particular to a multi-component essence with whitening and moisturizing effects and a preparation method thereof. Background Art

[0002] In the pursuit of beauty and youth, skin care is always an important link that we cannot ignore. With the change of seasons and the changes in the environment, the skin often faces multiple challenges such as dryness, dullness, and spots. In this context, it is of great significance to develop a skin care product with multiple functions such as whitening and moisturizing. Among many skin care products, essence is easy to use. Just take an appropriate amount of essence and gently massage it on the face and neck. In addition, the packaging design of essence is often more exquisite and portable, which is convenient for consumers to perform skin care anytime and anywhere. This convenience not only improves the skin care experience, but also makes it easier for consumers to stick to it and form skin care habits.

[0003] The whiteness of the skin is mainly determined by the melanin content, and tyrosinase is one of the important rate-limiting enzymes in the synthesis of melanocytes and pigments. Therefore, by inhibiting the activity of tyrosinase, a good whitening effect can be produced. Currently, there are many essences on the market with whitening and other functions, but most of these essences can only act on the surface and cannot penetrate into the skin to achieve targeted inhibition of tyrosinase. Therefore, it is actually difficult to achieve a long-term whitening effect.

[0004] Studies have found that luteolin has a good effect in inhibiting tyrosinase activity. Therefore, tyrosinase activity can be inhibited by adding luteolin to the essence formula. However, luteolin has poor compatibility with other components in the essence and is easily oxidized, making it difficult to use efficiently. Therefore, there are still obvious problems in practical applications. Summary of the invention

[0005] In order to solve the problems mentioned in the background technology, the object of the present invention is to provide a multi-component essence with whitening and moisturizing effects and a method for preparing the same.

[0006] The purpose of the present invention can be achieved through the following technical solutions:

[0007] A multi-component essence with whitening and moisturizing effects, comprising freeze-dried powder, freeze-dried powder B and essence C;

[0008] The freeze-dried powder A is prepared from raw materials including the following raw materials in parts by weight: 3-5 parts of vitamin C, 4-6 parts of reduced glutathione, 6-8 parts of mannitol, and 70-90 parts of water;

[0009] The freeze-dried powder B is prepared from the following raw materials in parts by weight: 0.005-0.01 parts of oligopeptide factor, 0.01-0.02 parts of nonapeptide-1, 3-5 parts of nicotinamide, 0.1-0.2 parts of trehalose, 0.4-0.6 parts of glycine, 4-6 parts of mannitol, 0.1-0.2 parts of disodium hydrogen phosphate, 0.05-0.1 parts of sodium dihydrogen phosphate, and 80-90 parts of water;

[0010] The essence C is prepared from the following raw materials in parts by weight: 1-5 parts of luteolin sustained-release agent, 0.4-0.6 parts of sodium hyaluronate, 0.1-0.2 parts of sodium carboxymethyl cellulose, 3-5 parts of propylene glycol, 4-6 parts of tranexamic acid, and 80-90 parts of water.

[0011] As a further embodiment of the present invention, the mass ratio of the lyophilized powder, lyophilized powder B and essence C is 1-3:1-3:10.

[0012] As a further embodiment of the present invention, the preparation method of the luteolin sustained-release agent comprises the following steps:

[0013] Step S1, dissolving polyglycerol fatty acid ester in ethanol to prepare a polyglycerol fatty acid ester mixed solution;

[0014] Step S2, adding luteolin and an emulsifier to the polyglycerol fatty acid ester mixture, controlling the temperature to 40-50° C., stirring at a rate of 2000-3000 r / min, and mechanically shearing and emulsifying for 5-10 minutes, then adding the formed mixture to 3-5 times the volume of water, and after the addition, standing at room temperature for 10-20 minutes to form a suspension;

[0015] Step S3, adding gum arabic to the suspension, controlling the rotation speed to 3500-4000 r / min, stirring at a temperature of 50-60° C. for 5-10 min, stopping heating, cooling and discharging the material, and freeze-drying the obtained mixture to obtain a luteolin sustained-release agent.

[0016] As a further solution of the present invention, in step S1, the mass fraction of the polyglycerol fatty acid ester mixed solution is 5-15%.

[0017] As a further solution of the present invention, in step S2, the emulsifier is Tween 60 or Tween 80.

[0018] As a further embodiment of the present invention, in step S2, in the mixed material, the mass ratio of luteolin to polyglycerol fatty acid ester is 0.1-0.15:1.

[0019] As a further solution of the present invention, in step S3, the temperature of the freeze-drying treatment is -50 to -60°C and the time is 12 to 24 hours.

[0020] A method for preparing a multi-component essence with whitening and moisturizing effects comprises the following steps:

[0021] The first step is to weigh the various raw materials of freeze-dried powder A according to the mass fraction, then add water into the stirring kettle, heat it to 80-85°C to form a precursor solution, control the stirring rate to 60-80r / min, add vitamin C, reduced glutathione and mannitol into the precursor solution, continue to stir after adding, mix evenly, cool down and discharge, put the collected liquid into a vial, freeze-dry at -5 to -60°C for 24 hours, and obtain freeze-dried powder A;

[0022] Step 2: Weigh each raw material in the freeze-dried powder B according to the mass fraction, then add water to the stirring kettle, heat it to 80-90°C, stir for 10-20min, then reduce the temperature to 40-45°C, control the speed to 60-80r / min, and continue to add oligopeptide factors, nonapeptide-1, nicotinamide, trehalose, glycine, mannitol, disodium hydrogen phosphate, and sodium dihydrogen phosphate under continuous stirring. After adding, continue to stir, mix evenly, cool and discharge, collect the liquid, put it into a vial, and freeze-dry it at -5 to -60°C for 24h to obtain freeze-dried powder B;

[0023] Step 3: Weigh the various raw materials in Essence C, then add sodium carboxymethyl cellulose, propylene glycol and sodium hyaluronate into water, control the temperature to 80-85°C, stir at 100-120r / min for 40-60min until completely dispersed, then lower the temperature to 40-45°C, add luteolin sustained-release agent and tranexamic acid to the precursor solution under continuous stirring, continue stirring after addition, mix evenly, cool and discharge, and obtain Essence C;

[0024] Step 4: Add freeze-dried powder A and freeze-dried powder B to essence C according to the mass ratio, control the stirring rate to 100-150r / min, and mechanically stir until the mixture is evenly mixed to obtain a multi-component essence.

[0025] Beneficial effects of the present invention:

[0026] The present invention designs a variety of component combinations to prepare a multi-component essence, wherein each component in freeze-dried powder A can play a good hydrating effect, each component in freeze-dried powder B can cooperate synergistically, accelerate melanin metabolism, and block melanin transmission, and the luteolin sustained-release agent uniquely prepared in essence C is a shell-core structure, the shell layer is polyglycerol fatty acid ester and gum arabic in sequence, and gum arabic is a polyelectrolyte, which can be adsorbed on the skin surface, so that the luteolin in the core layer is slowly released on the skin surface, enters the deep layer of the skin, and inhibits tyrosinase activity, thereby producing a synergistic effect with freeze-dried powder A and freeze-dried powder B, and achieving skin whitening from multiple angles. In addition, this coating structure can well avoid the oxidation of luteolin, and can improve the compatibility of luteolin with other components in the essence. In addition, the hyaluronic acid and other components in the essence C can penetrate into the bottom of the skin, form a water-locking network, effectively lock the moisture of the skin, prevent water loss, and make the essence show excellent hydrating and moisturizing effects.

[0027] Of course, any product implementing the present invention does not necessarily need to achieve all of the advantages described above at the same time. DETAILED DESCRIPTION

[0028] The technical scheme of the present invention will be clearly and completely described below in conjunction with the embodiments. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0029] Preparation Example 1

[0030] Preparation of luteolin sustained-release agent:

[0031] Step S1, dissolving polyglycerol fatty acid ester in ethanol to prepare a polyglycerol fatty acid ester mixed solution with a mass fraction of 10%;

[0032] Step S2, adding 1.2 g of luteolin and 0.5 g of Tween 60 to 100 mL of uniform liquid, controlling the temperature to 45° C., stirring at a rate of 2800 r / min, and mechanically shearing and emulsifying for 50 min, then adding the formed mixture to 5 times the volume of water, and after the addition, standing at room temperature for 15 min to form a suspension;

[0033] Step S3, adding 12 g of gum arabic to the suspension, controlling the rotation speed to 3800 r / min, stirring at 60° C. for 8 min, stopping heating, cooling and discharging the material, and freeze-drying the obtained mixture at -50° C. for 24 h to obtain a luteolin sustained-release agent.

[0034] In the above technical scheme, since the polyglycerol fatty acid ester is amphiphilic, ethanol is first used as a good solvent to dissolve it to form a mixed solution, and then luteolin is added to the mixed solution. Since luteolin can be well dissolved in ethanol, a hybrid liquid material can be formed. At this time, multiple volumes of water are added, and the system is rapidly converted from a good solvent to a poor solvent. The hydrophilic groups in the amphiphilic polyglycerol fatty acid ester structure will rapidly expand outward, and the hydrophobic groups will shrink inward to form a pore structure that is hydrophilic on the outside and hydrophobic on the inside. Similarly, luteolin will precipitate in the poor solvent and flow into the lipophilic pores to form a coated structure. Then, polyelectrolyte gum arabic is used for further coating to obtain a luteolin sustained-release agent with a double-layer shell structure.

[0035] Example 1

[0036] A multi-component essence with whitening and moisturizing effects, comprising freeze-dried powder, freeze-dried powder B and essence C;

[0037] The freeze-dried powder A is prepared from raw materials including the following raw materials in parts by weight: 3 parts of vitamin C, 4 parts of reduced glutathione, 6 parts of mannitol, and 70 parts of water;

[0038] The freeze-dried powder B is prepared from the following raw materials in parts by weight: 0.00 parts of oligopeptide factor, 0.01 parts of nonapeptide-1, 3 parts of nicotinamide, 0.1 parts of trehalose, 0.4 parts of glycine, 4 parts of mannitol, 0.1 parts of disodium hydrogen phosphate, 0.05 parts of sodium dihydrogen phosphate, and 80 parts of water;

[0039] The essence C is prepared from the following raw materials in parts by weight: 1 part of luteolin sustained-release agent, 0.4 parts of sodium hyaluronate, 0.12 parts of sodium carboxymethyl cellulose, 3 parts of propylene glycol, 4 parts of tranexamic acid, and 80 parts of water.

[0040] The preparation method of the multi-component essence comprises the following steps:

[0041] The first step is to weigh the various raw materials of freeze-dried powder A according to the mass fraction, then add water into the stirring kettle, heat it to 80°C to form a precursor solution, control the stirring rate to 80r / min, add vitamin C, reduced glutathione, and mannitol into the precursor solution, continue to stir after adding, mix evenly, cool down and discharge, put the collected liquid into a vial, and freeze-dry at -50°C for 24 hours to obtain freeze-dried powder A;

[0042] Step 2: Weigh each raw material in the freeze-dried powder B according to the mass fraction, then add water to the stirring kettle, heat to 85°C, stir for 20 minutes, then reduce the temperature to 45°C, control the speed to 80r / min, and continue to add oligopeptide factor, nonapeptide-1, nicotinamide, trehalose, glycine, mannitol, disodium hydrogen phosphate, and sodium dihydrogen phosphate under continuous stirring. After adding, continue to stir, mix evenly, cool down and discharge, collect the liquid, put it into a vial, and freeze-dry at -50°C for 24 hours to obtain freeze-dried powder B;

[0043] Step 3: Weigh each raw material in Essence C, then add sodium carboxymethyl cellulose, propylene glycol and sodium hyaluronate into water, control the temperature to 80°C, stir at 100r / min for 4 minutes until completely dispersed, then lower the temperature to 4°C, add luteolin sustained-release agent and tranexamic acid to the precursor solution under continuous stirring, continue stirring after adding, mix evenly, cool and discharge, and obtain Essence C;

[0044] Step 4: Add freeze-dried powder A and freeze-dried powder B to essence C in a mass ratio of 1:1:10, control the stirring rate to 100 r / min, and mechanically stir until the mixture is uniform to obtain a multi-component essence.

[0045] The preparation method of luteolin sustained-release agent is shown in Preparation Example 1.

[0046] Example 2

[0047] A multi-component essence with whitening and moisturizing effects, comprising freeze-dried powder, freeze-dried powder B and essence C;

[0048] The freeze-dried powder A is prepared from raw materials including the following raw materials in parts by weight: 4 parts of vitamin C, 5 parts of reduced glutathione, 7 parts of mannitol, and 80 parts of water;

[0049] The freeze-dried powder B is prepared from the following raw materials in parts by weight: 0.008 parts of oligopeptide factor, 0.01 parts of nonapeptide-1, 4 parts of nicotinamide, 0.15 parts of trehalose, 0.4-0.6 parts of glycine, 5 parts of mannitol, 0.15 parts of disodium hydrogen phosphate, 0.06 parts of sodium dihydrogen phosphate, and 85 parts of water;

[0050] The essence C is prepared from the following raw materials in parts by weight: 2 parts of luteolin sustained-release agent, 0.5 parts of sodium hyaluronate, 0.1 parts of sodium carboxymethyl cellulose, 4 parts of propylene glycol, 5 parts of tranexamic acid, and 85 parts of water.

[0051] The preparation method of the multi-component essence is the same as that of Example 1.

[0052] Example 3

[0053] A multi-component essence with whitening and moisturizing effects, comprising freeze-dried powder, freeze-dried powder B and essence C;

[0054] The freeze-dried powder A is prepared from raw materials including the following raw materials in parts by weight: 5 parts of vitamin C, 6 parts of reduced glutathione, 8 parts of mannitol, and 90 parts of water;

[0055] The freeze-dried powder B is prepared from the following raw materials in parts by weight: 0.01 parts of oligopeptide factor, 0.02 parts of nonapeptide-1, 5 parts of nicotinamide, 0.2 parts of trehalose, 0.6 parts of glycine, 6 parts of mannitol, 0.2 parts of disodium hydrogen phosphate, 0.1 parts of sodium dihydrogen phosphate, and 90 parts of water;

[0056] The essence C is prepared from the following raw materials in parts by weight: 1 part of luteolin sustained-release agent, 0.6 parts of sodium hyaluronate, 0.2 parts of sodium carboxymethyl cellulose, 3 parts of propylene glycol, 6 parts of tranexamic acid, and 90 parts of water.

[0057] The preparation method of the multi-component essence is the same as that of Example 1.

[0058] Comparative Example 1

[0059] A multi-component essence with whitening and moisturizing effects, which is different from Example 2 in that the luteolin sustained-release agent is replaced with luteolin, and the rest remains unchanged.

[0060] Test Case

[0061] The whitening effect of the essence was tested by selecting B16F10 mouse melanoma cells, which have the same function as the normal melanin synthesis of the human body, and inoculated in a fetal bovine serum culture medium with a volume fraction of 10%, cultured at 37°C, and digested and passaged with trypsin with a mass fraction of 0.25% every other day. The second generation of melanoma cells were taken and placed in a 12-well plate at a seeding density of 100 cells / mL. The liquid was changed overnight, and then 2 mL of the essence in each embodiment and comparative example was added to the well plate. At the same time, a control group was set up, and the control group was not treated. After the addition, the cells were cultured at 37°C for 3 days, the supernatant was skimmed off, and 1 mL was used. PBS buffer was used for washing, and then 100 μL of trypsin was added. After digestion for 10 minutes, PBS buffer was added to stop digestion, the supernatant was removed, and then 0.1% polyethylene glycol octylphenyl ether by mass was added to each well, shaken, stored in an environment of minus 20°C for 20 minutes, and then placed at a temperature of 5°C for 30 minutes, the supernatant was removed again, and finally 1% sodium hydroxide dimethyl sulfoxide solution by mass was added, and kept warm at 80°C for 3 hours. The supernatant was placed in a 96-well plate, and the absorbance value at a wavelength of 490nm was read using an enzyme reader. By comparing with the control group, the melanin reduction rate was calculated, and the results are recorded in Table 2:

[0062] Group Melanin reduction rate / % Example 1 Group 82.41 Example 2 Group 82.50 Example 3 Group 82.39 Comparative Example 1 68.44

[0063] Analysis shows that the essence prepared by using luteolin-encapsulated luteolin sustained-release agent as an additive has a significantly stronger whitening effect than the essence prepared by directly adding luteolin. This is because luteolin is easily oxidized and its efficacy is difficult to fully exert.

[0064] Specific examples are used herein to illustrate the principles and implementation methods of the present invention. The description of the above embodiments is only used to help understand the method of the present invention and its core ideas, including the best mode, and also enable any technician in the field to practice the present invention, including making and using any device or system, and implementing any combined method. It should be pointed out that for ordinary technicians in this technical field, without departing from the principle of the present invention, the present invention can also be improved and modified, and these improvements and modifications also fall within the scope of protection of the claims of the present invention. The scope of patent protection of the present invention is defined by the claims and may include other embodiments that can be thought of by those skilled in the art. If these other embodiments have structural elements similar to the literal expression of the claims, or if they include equivalent structural elements that are not substantially different from the literal expression of the claims, then these other embodiments should also be included in the scope of the claims.

[0065] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. Such modifications or replacements will not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A multi-component essence with whitening and moisturizing effects, characterized in that: Including freeze-dried powder, freeze-dried powder B and essence C; The freeze-dried powder A is prepared from raw materials including the following raw materials in parts by weight: 3-5 parts of vitamin C, 4-6 parts of reduced glutathione, 6-8 parts of mannitol, and 70-90 parts of water; The freeze-dried powder B is prepared from the following raw materials in parts by weight: 0.005-0.01 parts of oligopeptide factor, 0.01-0.02 parts of nonapeptide-1, 3-5 parts of nicotinamide, 0.1-0.2 parts of trehalose, 0.4-0.6 parts of glycine, 4-6 parts of mannitol, 0.1-0.2 parts of disodium hydrogen phosphate, 0.05-0.1 parts of sodium dihydrogen phosphate, and 80-90 parts of water; The essence C is prepared from the following raw materials in parts by weight: 1-5 parts of luteolin sustained-release agent, 0.4-0.6 parts of sodium hyaluronate, 0.1-0.2 parts of sodium carboxymethyl cellulose, 3-5 parts of propylene glycol, 4-6 parts of tranexamic acid, and 80-90 parts of water.

2. The multi-component essence with whitening and moisturizing effects according to claim 1, characterized in that: The mass ratio of the freeze-dried powder, freeze-dried powder B and essence C is 1-3:1-3:

10.

3. The multi-component essence with whitening and moisturizing effects according to claim 1, characterized in that: The preparation method of the luteolin sustained-release agent comprises the following steps: Step S1, dissolving polyglycerol fatty acid ester in ethanol to prepare a polyglycerol fatty acid ester mixed solution; Step S2, adding luteolin and an emulsifier to the polyglycerol fatty acid ester mixture, controlling the temperature to 40-50° C., stirring at a rate of 2000-3000 r / min, and mechanically shearing and emulsifying for 5-10 minutes, then adding the formed mixture to 3-5 times the volume of water, and after the addition, standing at room temperature for 10-20 minutes to form a suspension; Step S3, adding gum arabic to the suspension, controlling the rotation speed to 3500-4000 r / min, stirring at a temperature of 50-60° C. for 5-10 min, stopping heating, cooling and discharging the material, and freeze-drying the obtained mixture to obtain a luteolin sustained-release agent.

4. The multi-component essence with whitening and moisturizing effects according to claim 3, characterized in that: In step S1, the mass fraction of the polyglycerol fatty acid ester mixed solution is 5-15%.

5. The multi-component essence with whitening and moisturizing effects according to claim 3, characterized in that: In step S2, the emulsifier is Tween 60 or Tween 80.

6. The multi-component essence with whitening and moisturizing effects according to claim 3, characterized in that: In step S2, in the mixed material, the mass ratio of luteolin to polyglycerol fatty acid ester is 0.1-0.15:

1.

7. The multi-component essence with whitening and moisturizing effects according to claim 3, characterized in that: In step S3, the temperature of the freeze-drying treatment is -50 to -60°C and the time is 12 to 24 hours.

8. A method for manufacturing a multi-component essence with whitening and moisturizing effects as claimed in claim 1, characterized in that: The following steps are involved: The first step is to weigh the various raw materials of freeze-dried powder A according to the mass fraction, then add water into the stirring kettle, heat it to 80-85°C to form a precursor solution, control the stirring rate to 60-80r / min, add vitamin C, reduced glutathione and mannitol into the precursor solution, continue to stir after adding, mix evenly, cool down and discharge, put the collected liquid into a vial, freeze-dry at -5 to -60°C for 24 hours, and obtain freeze-dried powder A; Step 2: Weigh each raw material in the freeze-dried powder B according to the mass fraction, then add water to the stirring kettle, heat it to 80-90°C, stir for 10-20min, then reduce the temperature to 40-45°C, control the speed to 60-80r / min, and continue to add oligopeptide factors, nonapeptide-1, nicotinamide, trehalose, glycine, mannitol, disodium hydrogen phosphate, and sodium dihydrogen phosphate under continuous stirring. After adding, continue to stir, mix evenly, cool and discharge, collect the liquid, put it into a vial, and freeze-dry it at -5 to -60°C for 24h to obtain freeze-dried powder B; Step 3: Weigh the various raw materials in Essence C, then add sodium carboxymethyl cellulose, propylene glycol and sodium hyaluronate into water, control the temperature to 80-85°C, stir at 100-120r / min for 40-60min until completely dispersed, then lower the temperature to 40-45°C, add luteolin sustained-release agent and tranexamic acid to the precursor solution under continuous stirring, continue stirring after addition, mix evenly, cool and discharge, and obtain Essence C; Step 4: Add freeze-dried powder A and freeze-dried powder B to essence C according to the mass ratio, control the stirring rate to 100-150r / min, and mechanically stir until the mixture is evenly mixed to obtain a multi-component essence.