A composite preparation for fasting-induced physiological remodeling in laying hens and its application

Abstract

The present invention discloses a composite formulation for fasting-induced physiological remodeling in laying hens and its application, belonging to the technical field of fasting-induced physiological remodeling. The composite formulation and method provided by the present invention can significantly increase the ileal villus height, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes of laying hens, significantly reduce the expression levels of intestinal pro-inflammatory-related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens; can significantly increase the expression levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M, and thus improve the immune performance of laying hens; with the improvement of intestinal health and immunity, fasting-induced physiological remodeling of laying hens is successfully achieved: the weight and egg production recovery ability of laying hens after fasting, and the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical support for the extended breeding of laying hens, saving costs and increasing efficiency.

Description

Technical Field

[0001] The present invention relates to the technical field of fasting-induced physiological remodeling, and in particular to a composite preparation for fasting-induced physiological remodeling in laying hens and applications thereof. Background Art

[0002] In recent years, with the rising prices of feed ingredients such as corn and soybean meal, extending the lifespan of laying hens has become a global trend. A key task in my country's laying hen industry is to extend the laying cycle, achieving 500 eggs per 700 days while maintaining high egg production and egg quality in the later stages of the laying period. Implementing fast-induced physiological remodeling (FIPR) is one important approach to achieving this goal.

[0003] Fasting-induced physiological remodeling is a method that uses fasting to induce remodeling of various body functions in laying hens. The shedding and regeneration of feathers (commonly known as forced molting) is a prominent feature of this process. During fasting-induced physiological remodeling, laying hens undergo significant physiological and metabolic changes. For example, it regulates follicular development. Through fasting, the ovarian function and follicular development of laying hens change, promoting the development of primary and secondary follicles, thereby extending the laying cycle of laying hens and bringing a second peak of egg production to the flock. It also affects the synthesis, secretion, and uptake of bile acids and effectively alleviates fatty liver caused by metabolic disorders in older laying hens. However, during the fasting-induced physiological remodeling process, it also induces immunosuppression in laying hens, increases sensitivity to pathogens such as Salmonella enteritidis and Escherichia coli, and leads to problems such as intestinal flora disorders and severe intestinal damage. Corresponding intestinal health care programs are relatively rare. Other studies have found that during the process of induced physiological remodeling, the intestinal structure is destroyed during fasting, and the content of beneficial intestinal bacteria Bacteroides and its synthetic metabolite vitamin B6 is significantly reduced. Summary of the Invention

[0004] The purpose of the present invention is to provide a composite preparation for fasting-induced physiological remodeling in laying hens and its application, so as to solve the problem in the prior art that fasting-induced physiological remodeling in laying hens easily induces immunosuppression, intestinal flora disorder, and severe intestinal damage.

[0005] To achieve the above object, the present invention provides a composite preparation for fasting-induced physiological remodeling in laying hens, the composite preparation comprising Bacteroides fragilis ATCC25285+vitamin B6, and the number of viable bacteria of Bacteroides fragilis ATCC25285 in the composite preparation is 1×10 9 CFU / mL, the mass of vitamin B6 is 0.5 mg.

[0006] Preferably, Bacteroides fragilis ATCC25285 is activated using brain heart infusion broth culture medium, and the pH of the culture medium is 7.4±0.2.

[0007] A method for fasting-induced physiological remodeling in laying hens, using the composite preparation for fasting-induced physiological remodeling in laying hens as described above, comprises the following steps:

[0008] S1. Select healthy laying hens in the late egg-laying stage and perform fasting induction. Cut off water and feed supply on the first 1-3 days of fasting. The laying hens should be exposed to light for 8 hours per day. Avoid the flow of people and noise pollution near the chicken house.

[0009] S2. From the 4th to the 15th day of fasting, the feed supply was interrupted, water was provided normally, and a compound preparation for fasting-induced physiological remodeling was administered orally to the laying hens. The laying hens were exposed to light for 8 hours per day.

[0010] S3. When the weight loss rate of laying hens is ≥25%, start to resume feeding and gradually extend the lighting time by 30 minutes per day until the 16th hour, and stop extending it after 16 hours.

[0011] Preferably, each laying hen in S2 is gavage-administered 1 mL of the composite preparation for fasting-induced physiological remodeling in laying hens.

[0012] Preferably, in S2, fasting is ended early when the laying hen mortality rate reaches 4% or above.

[0013] Preferably, the area around the chicken house is sterilized every day during the fasting period.

[0014] Preferably, the feeding resumption method in S3 is: feeding 30 g / head basal feed per day on the 1st to 2nd day of feeding resumption; feeding 60 g / head basal feed per day on the 3rd to 4th day of feeding resumption; feeding 90 g / head basal feed per day on the 5th day of feeding resumption; feeding 120 g / head basal feed per day on the 6th to 20th day of feeding resumption; and ad libitum feeding thereafter.

[0015] An application of the composite preparation for fasting-induced physiological remodeling in laying hens as described above in fasting-induced physiological remodeling in laying hens.

[0016] Bacteroides fragilis ATCC25285 is considered a beneficial bacterium and a vital component of the intestinal flora. It contributes to maintaining intestinal homeostasis and plays a crucial role in promoting immune system maturation, suppressing intestinal inflammation, and improving intestinal flora structure. It is commonly found in the human intestinal flora and is also present in the oral cavity and upper respiratory tract. Vitamin B6 is an essential micronutrient for the healthy growth of animals and has physiological functions such as anti-inflammatory, antioxidant, neuromodulatory, and anti-tumor. However, no studies on the combined use of Bacteroides fragilis ATCC25285 and vitamin B6 have been found.

[0017] Therefore, the present invention provides a composite preparation for fasting-induced physiological remodeling in laying hens and its application, and its specific technical effects are as follows:

[0018] (1) The composite preparation and method provided by the present invention can successfully achieve fasting-induced physiological remodeling of laying hens: the weight and egg production recovery ability of laying hens after fasting, as well as the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical support for extending the breeding of laying hens, saving costs and increasing efficiency;

[0019] (2) The composite preparation and method provided by the present invention can significantly increase the ileal villus height, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes of laying hens, significantly reduce the expression levels of intestinal pro-inflammatory-related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens.

[0020] (3) The composite preparation and method provided by the present invention can significantly increase the expression levels of immunoglobulins, immunoglobulin G, and immunoglobulin M, thereby improving the immune performance of laying hens. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for use in the description of the embodiments of the present invention. Obviously, the drawings described below are only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0022] Figure 1 Statistical results of egg production rates at different fasting periods in the embodiment of the present invention;

[0023] Figure 2 5 is a morphological and histological section diagram of the jejunum of laying hens at different stages in an embodiment of the present invention (HE5x);

[0024] Figure 3 1 is a morphological and histomorphological section diagram of the ileum of laying hens at different stages in an embodiment of the present invention (HE5x);

[0025] Figure 4It is the expression level of related genes in the jejunum of laying hens in the embodiment of the present invention, wherein Part A is the relative expression level of TNF-α at different stages, Part B is the relative expression level of IL-15 at different stages, Part C is the relative expression level of IL-6 at different stages, Part D is the relative expression level of IL-18 at different stages, Part E is the relative expression level of Claudin-1 at different stages, Part F is the relative expression level of occludin-1 at different stages, Part G is the relative expression level of Lgr5 at different stages, and Part H is the relative expression level of CDK1 at different stages;

[0026] Figure 5 It is the expression level of ileum-related genes in laying hens in the embodiment of the present invention, wherein Part A is the relative expression level of TNF-α at different periods, Part B is the relative expression level of IL-15 at different periods, Part C is the relative expression level of IL-6 at different periods, Part D is the relative expression level of IL-18 at different periods, Part E is the relative expression level of Claudin-1 at different periods, Part F is the relative expression level of occludin-1 at different periods, Part G is the relative expression level of Lgr5 at different periods, and Part H is the relative expression level of CDK1 at different periods. DETAILED DESCRIPTION

[0027] The technical solution of the present invention is further described below with reference to the accompanying drawings and embodiments.

[0028] In order to make the purpose, technical solutions and advantages of the present application clearer, more thorough and more complete, the technical solutions of the present invention are clearly and completely described below through the accompanying drawings and Examples. The following detailed description is an explanation of the embodiments and is intended to provide further details of the present invention. Unless otherwise specified, all technical terms used in the present invention have the same meaning as those generally understood by those skilled in the art to which the application belongs.

[0029] The instruments, equipment, reagents and materials used in the examples were obtained from commercial sources. The experimental methods in the examples, unless otherwise specified, are conventional methods in the art.

[0030] Example 1

[0031] Fasting-induced physiological remodeling was performed on laying hens. The specific settings are shown in Table 1. The specific steps are as follows:

[0032] Table 1

[0033]

[0034] S11. A total of 288 healthy, disease-free bantam chickens (420 days old, weighing 1300-1500 g) were selected and randomly divided into four groups, with 9 replicates in each group and 8 chickens in each replicate, totaling 72 chickens in each group. One group was the control group, and three groups were the treatment groups: Bacteroides fragilis ATCC25285 group, vitamin B6 group, and Bacteroides fragilis ATCC25285 + vitamin B6 combination group.

[0035] S12. The experimental chicken house was strictly disinfected by fumigation with formaldehyde and potassium permanganate. The laying hens selected in step S11 were then transferred to the chicken house and fed a conventional basal diet (adaptive feeding) for 10 days. The composition and nutritional level of the basal diet are shown in Table 2.

[0036] Table 2

[0037] project ％ Nutritional levels ％ corn 62.50 Metabolic energy 11.20 soybean meal 25.50 crude protein 15.80 Stone powder 9.20 calcium 3.78 Oil powder 0.80 Available phosphorus 0.28 Premix 2.00 Lysine 0.71 total 100.00 Methionine 0.66

[0038] The premix provides VA12,000 IU, VD33,000 IU, VE30 IU, VK10 mg, VB112 mg, VB120.05 mg, biotin 16.4 mg, niacin 30 mg, pantothenic acid 10 mg, folic acid 1 mg, choline 500 mg, Cu45 mg, Fe80 mg, Zn85 mg, Mn70 mg, I0.5 mg, and Se0.0 mg per kilogram of feed. Crude protein and calcium are measured values, while other nutrient levels are calculated values.

[0039] S13. Then add 1% shell powder to the basic feed and add multivitamin (Yipan) to the drinking water and feed for ten days. Adding 1% shell powder can reduce the egg breaking rate after the start of molting and the overdraft of blood calcium in the chicken after molting. Adding multivitamin to the drinking water can prevent stress response.

[0040] S14. Number the chickens in each treatment group, randomly select 10 chickens from each treatment group, and monitor the weight changes of the numbered chickens in the later stage to estimate the weight changes of the entire chicken group. The day before the start of fasting induction (F0), the weight and number of eggs of the laying hens in the control group and the experimental group were weighed and recorded, and the egg production rate (total number of eggs / total number of laying hens) was calculated. The feed trough was cleaned and the water in the water line was drained.

[0041] The weight of F0 laying hens was the average weight of 40 laying hens randomly selected from the four treatment groups, and the egg production rate of F0 was the average egg production rate of 288 laying hens in the experiment. The average weight of F0 was 1248 g and the egg production rate was 37.35%.

[0042] S15. Fasting induction period (F) settings are shown in Table 1:

[0043] (1) On the first 3 days after fasting induction, water and feed (basic feed) were cut off, and the light was on for 8 hours. During this period, the flow of people in the chicken house was reduced as much as possible, the environment was kept quiet, and the influence of other external factors except water and feed cutoff was avoided.

[0044] (2) From the 4th to the 15th day after the fasting induction, the feed and water were cut off. The control group was gavaged with normal saline, and the Bacteroides fragilis ATCC25285 group was gavaged with 1×10 viable bacteria per day. 9 The vitamin B6 group was orally administered with 0.5 mg of vitamin B6 daily, and the combined preparation group was orally administered with 1×10 Bacteroides fragilis ATCC25285 live bacteria. 9 CFU live bacteria preparation + 0.5mg vitamin B6, light exposure for 8h.

[0045] S16. After the fasting induction began, the weights of the laying hens in the control and treatment groups were weighed and recorded at 4 p.m. every three days. The weight loss rate was calculated using the following formula (results are shown in Table 4). The egg production rate was calculated and recorded (results are shown in Table 4). Figure 1 When the average weight loss rate of the laying hens reaches 25%, they can resume feeding. The weight loss rate is calculated using the following formula:

[0046] Weight loss rate of Fx (%) = (weight at F0 - weight at Fx) / weight at F0 × 100%, where F is the fasting period, F0 is the day before the start of fasting, and Fx is the xth day of fasting.

[0047] Based on the monitoring of the chicken flock weight, the fasting period was determined to be 15 days.

[0048] S17, recovery feeding period (R), the 16th day after the fasting induction period (recorded as the first day of the recovery feeding period, R1) enters the recovery feeding period. During the recovery feeding period, the chickens drink water normally. The feeding conditions during the recovery feeding period are as follows:

[0049] On the first 1-2 days after the resumption of feeding, feed 30g of basic feed per head per day;

[0050] On the 3rd to 4th day after the resumption of feeding, feed 60g of basic feed per head per day;

[0051] On the fifth day of resuming feeding, the animals were fed 90 g of basic feed per day;

[0052] Feed 120g of basic feed per head per day from day 6 to day 20 after resuming feeding;

[0053] Thereafter, the animals were fed ad libitum.

[0054] During the gradual recovery of feeding, the light intensity was increased by 30 min per day until it reached 16 h / day.

[0055] Example 2

[0056] The production performance and egg quality of laying hens were investigated one day before fasting, during the fasting induction period, and during the recovery period. The specific steps are as follows:

[0057] S21. Record the number of eggs laid by each group of laying hens at 16:00 every day, and then take the average of the daily egg production rates in the five stages F0-F3, F4-F5, R17-R22, R23-R26, and R27-R30 as the egg production rate for this stage; record the body weight of each group every three days and calculate the weight loss rate.

[0058] Rx weight loss rate (%) = (weight at F0 - Rx weight) / F0 weight × 100%, where

[0059] R is the recovery period, F0 is the day before the start of fasting, and Rx represents the xth day of resuming feeding.

[0060] S22. During the R30 period, i.e., the 30th day after the end of fasting, 25 eggs were randomly collected from each group for determination of egg quality indicators using a multifunctional egg quality analyzer. The measured indicators included egg weight, egg shape index, eggshell thickness, eggshell strength, eggshell weight, egg white height, and yolk color.

[0061] Example 3

[0062] The immune function, intestinal tissue morphology, and intestinal-related gene expression levels of laying hens were investigated one day before fasting, during the fasting induction period, and during the recovery period. The specific steps are as follows:

[0063] S31. One laying hen with a near-average weight was selected from each replicate in the treatment and control groups, F0, F15, R5, and R30, for a total of 9 replicates. Blood was collected from the subwing vein using a coagulant tube, and serum was collected after centrifugation at 1265×g for 10 min. The serum was quickly frozen in liquid nitrogen and stored at -80°C for the detection of serum immune indicators.

[0064] The levels of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) were detected using an ELASA kit.

[0065] S32. After blood collection, the experimental chickens were slaughtered and 1 cm of the jejunum and ileum were collected and slowly rinsed with 0.9% saline. The collected small intestines were placed in a 10 mL centrifuge tube containing 4% paraformaldehyde (fixative) for fixation. After the collected jejunum and ileum segments were fixed for 24 hours, the intestinal segments were removed from the fixative and embedded in paraffin. The sections were made and stained with hematoxylin-eosin (HE). The sections were observed under an optical microscope and photographed. The results are shown in Figure 3. Figure 2 、 3The villus height and crypt depth of each intestinal segment were measured using MoticDS Assistant Lite software, and the villus crypt ratio (villus height / crypt depth) was calculated. The results are shown in Table 6.

[0066] S33. Collect jejunal and ileal tissue samples, place them in 1.5 mL sterile enzyme-free centrifuge tubes, quickly freeze them in liquid nitrogen, and finally store them in a -80°C refrigerator for subsequent measurements.

[0067] The total RNA of jejunum and ileum of each sampling point was extracted using a kit, and the target gene was detected by real-time fluorescence quantitative PCR using glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as the internal reference gene. -ΔΔCt The relative mRNA expression of target genes in the intestinal segment was calculated by the method. The results are as follows Figure 4 and Figure 5 The primer sequences and fragment sizes of the reference and target genes are shown in Table 3.

[0068] Table 3

[0069]

[0070]

[0071] Result Analysis

[0072] The experimental data were initially collated and analyzed using Excel 2010, followed by SPSS 26.0 software. One-way analysis of variance and Duncan's method were used for multiple comparisons of production performance and intestinal tissue morphology. Student's t-tests were used to analyze intestinal gene expression and serum immune parameters. Results are expressed as mean ± standard deviation, with P < 0.05 indicating a significant difference. GraphPad Prism 8.0 software was used for data processing and graphics.

[0073] In the table, data in the same row with different lowercase letters indicate significant differences (P<0.05). The control group is represented by NC, Bacteroides fragilis ATCC25285 is represented by B. fragilis, vitamin B6 is represented by VB6, and the combined preparation group of Bacteroides fragilis ATCC25285 and vitamin B6 is represented by B. fragilis+VB6.

[0074] (1) The statistical results of weight loss rate (%) of laying hens in the experimental group and the control group are shown in Table 4.

[0075] On the 45th day, i.e., during the R30 period, the weight recovery of the Bacteroides fragilis ATCC25285+vitamin B6 combination preparation group was better than that of the other groups, but there was no significant difference between the groups at each stage (P>0.05).

[0076] Table 4

[0077]

[0078]

[0079] (2) Effects of Bacteroides fragilis ATCC25285 and vitamin B6 on egg production in laying hens during fasting-induced physiological remodeling Figure 1 As shown, within F1-F3, the average egg production rate of the NC group decreased to 25.51%, the average egg production rate of the Bacteroides fragilis ATCC25285 group decreased to 28.70%, the average egg production rate of the vitamin B6 group decreased to 31.48%, and the average egg production rate of the Bacteroides fragilis ATCC25285 + vitamin B6 combination group decreased to 27.89%. Within F4-F5, the average egg production rate of the NC group decreased to 2.55%, the average egg production rate of the Bacteroides fragilis ATCC25285 group decreased to 2.09%, the average egg production rate of the vitamin B6 group decreased to 4.23%, and the average egg production rate of the Bacteroides fragilis ATCC25285 + vitamin B6 combination group decreased to 1.41%. F6-R17 flocks began to enter the dormant period. The NC group, Bacteroides fragilis ATCC25285 group, vitamin B6 group and Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group began to lay eggs again at R20, R18, R21 and R19, respectively, and the egg production rate of each group gradually increased. From R17 to R22, the average egg production rate of the NC group was 7.08%, the average egg production rate of the Bacteroides fragilis ATCC25285 group was 10.37%, the average egg production rate of the vitamin B6 group was 7.96%, and the average egg production rate of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was 10.71%. From R23 to R26, the NC group The average egg production rate of the NC group was 15.27%, the average egg production rate of the Bacteroides fragilis ATCC25285 group was 20.21%, the average egg production rate of the vitamin B6 group was 15.91%, and the average egg production rate of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was 20.07%; within R27-R30, the average egg production rate of the NC group was 31.83%, the average egg production rate of the Bacteroides fragilis ATCC25285 group was 39.54%, the average egg production rate of the vitamin B6 group was 31.17%, and the average egg production rate of the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was 42.64%.

[0080] (3) The effects of Bacteroides fragilis ATCC25285 and vitamin B6 on egg quality during fasting-induced physiological remodeling are shown in Table 5. Compared with R30NC, the yolk color of the Bacteroides fragilis ATCC25285 + vitamin B6 combination group was significantly darker (P < 0.05); the eggshell weight and eggshell strength of the vitamin B6 group were significantly improved (P < 0.05), and there were no significant differences in egg weight, egg shape index, and albumen height among the groups (P > 0.05).

[0081] Table 5

[0082]

[0083] (4) The effects of Bacteroides fragilis ATCC25285 and vitamin B6 on the intestinal morphology of laying hens during fasting-induced physiological remodeling are shown in Table 6. Compared with the control group, the crypt depth of the jejunum in the vitamin B6 group was significantly thickened at F15 (P < 0.05); the villus height of the ileum in the Bacteroides fragilis ATCC25285 + vitamin B6 combination group was significantly increased (P < 0.05). The villus height, crypt depth, and villus-crypt ratio of the intestine at other stages were not significant (P > 0.05).

[0084] Table 6

[0085]

[0086]

[0087] Note: The unit of villus height is μm, and the unit of crypt depth is μm.

[0088] (5) The effects of Bacteroides fragilis ATCC25285 and vitamin B6 on the immune indicators of laying hens during fasting-induced physiological remodeling were investigated. The results are shown in Table 7. IgA in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly higher than that in the control group during the R5 period (P<0.05); IgG in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly higher than that in the control group during the F15, R5, and R30 periods (P<0.05); IgM in the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group was significantly higher than that in the control group during the R5 period (P<0.05).

[0089] Table 7

[0090]

[0091]

[0092] (6) Effects of Bacteroides fragilis ATCC25285 and vitamin B6 on the expression of genes related to the jejunum and ileum of laying hens during fasting-induced physiological remodeling. Figure 4 、 5 Compared with the control group, the expression levels of intestinal barrier-related genes occludin-1 and Claudin-1, and intestinal proliferation-related genes CDK1 and Lgr5 in the Bacteroides fragilis ATCC25285+vitamin B6 combination preparation group were significantly increased (P<0.05), and the expression levels of intestinal pro-inflammatory genes TNF-α, IL-6, and IL-18 were significantly decreased (P<0.05).

[0093] The results showed that during the fasting-induced physiological remodeling process of laying hens, the Bacteroides fragilis ATCC25285+vitamin B6 compound preparation was superior to the treatment of adding Bacteroides fragilis ATCC25285 and vitamin B6 alone. The Bacteroides fragilis ATCC25285+vitamin B6 compound preparation group could improve the production performance of laying hens during the fasting-induced physiological remodeling process, improve their intestinal tissue morphology and structure, enhance intestinal barrier function and proliferation-related genes, reduce the expression of intestinal inflammation-related genes, enhance the body's immune function, and thus maintain the intestinal health of laying hens.

[0094] Therefore, the composite preparation and method provided by the present invention can significantly increase the ileal villus height, the expression levels of intestinal barrier-related genes and intestinal proliferation-related genes of laying hens, significantly reduce the expression levels of intestinal pro-inflammatory-related genes, alleviate intestinal stress damage and inflammation, and thus significantly improve the intestinal health of laying hens; can significantly increase the expression levels of immunoglobulin A, immunoglobulin G, and immunoglobulin M, and thus improve the immune performance of laying hens; with the improvement of intestinal health and immunity, fasting-induced physiological remodeling of laying hens is successfully achieved: the weight and egg production rate recovery ability of laying hens after fasting, and the yolk color depth on the 30th day after fasting are significantly better than those of the control group, providing technical guarantee for the extended breeding of laying hens, cost saving and efficiency improvement.

[0095] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit the same. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solutions of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solutions to deviate from the spirit and scope of the technical solutions of the present invention.

Claims

1. A composite preparation for fasting-induced physiological remodeling in laying hens, characterized by: The compound preparation is Bacteroides fragilis ATCC25285 + vitamin B6. The number of viable bacteria of Bacteroides fragilis ATCC25285 in the compound preparation is 1×10 9 CFU / mL, the mass of vitamin B6 is 0.5 mg.

2. The composite preparation for fasting-induced physiological remodeling in laying hens according to claim 1, characterized in that: Bacteroides fragilis ATCC25285 was activated using brain heart infusion broth at a pH of 7.4±0.

2.

3. A method for fasting-induced physiological remodeling in laying hens, using the composite preparation for fasting-induced physiological remodeling in laying hens according to claim 1, characterized in that: Here are the steps: S1. Select healthy laying hens in the late egg-laying stage and perform fasting induction. Cut off water and feed supply on the first 1-3 days of fasting. The laying hens should be exposed to light for 8 hours per day. Avoid the flow of people and noise pollution near the chicken house. S2: From the 4th to the 15th day of fasting, the feed supply was interrupted, water was provided normally, and a compound preparation for fasting-induced physiological remodeling was administered orally to the laying hens. The laying hens were exposed to light for 8 hours per day. S3. When the laying hen's weight loss rate is ≥25%, start to resume feeding and gradually extend the lighting time by 30 minutes per day until the 16th hour, and stop extending it after 16 hours.

4. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: In S2, each laying hen was gavage-administered 1 mL of the compound preparation for fasting-induced physiological remodeling in laying hens.

5. The method for inducing physiological remodeling in laying hens by fasting according to claim 3, characterized in that: In S2, fasting was terminated early when the mortality rate of laying hens reached 4% or above.

6. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: During the fasting period, sterilize the area around the chicken house every day.

7. The method for fasting-induced physiological remodeling in laying hens according to claim 3, characterized in that: The feeding resumption method in S3 is as follows: feeding 30g / head per day of basic feed on the 1st to 2nd day of feeding resumption; feeding 60g / head per day of basic feed on the 3rd to 4th day of feeding resumption; feeding 90g / head per day of basic feed on the 5th day of feeding resumption; feeding 120g / head per day of basic feed on the 6th to 20th day of feeding resumption; after that, free feeding.

8. Use of the composite preparation for fasting-induced physiological remodeling in laying hens as claimed in claim 1 in fasting-induced physiological remodeling in laying hens.

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