Cell extract centrifugal equipment for dental stem cells

Abstract

The invention discloses cell extract centrifugal equipment for dental stem cells, and belongs to the technical field of cell culture. The device mainly comprises a centrifugal table, a plurality of centrifugal frames are distributed on the circumferential side wall of the centrifugal table, a cover plate is fixed to the upper side of the centrifugal table, guide rails corresponding to the centrifugal frames in position are distributed on the upper side of the cover plate, a first sliding block and a second sliding block are arranged on the guide rails in a sliding mode, and the second sliding block is located on the side, close to the center of the cover plate, of the first sliding block. A hose is fixed to the end, close to the first sliding block, of the second sliding block, a hose hole is formed in the first sliding block, the end, close to the second sliding block, of the first sliding block is communicated with the lower end of the first sliding block through the hose hole, and the end, away from the second sliding block, of the hose extends to the lower side of the first sliding block through the hose hole and is fixedly provided with a balancing weight. According to the cell extract centrifugal equipment for the dental stem cells, the effect of extracting supernatant liquid is achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of cell culture, and more particularly to a cell extract centrifugal device for dental stem cells. Background Art

[0002] As the research on stem cells deepens, there are also some problems. For example, embryonic stem cells are subject to ethical and legal restrictions, and bone marrow stem cells are limited in their wide clinical application due to their source. In contrast, dental stem cells are not only easy to obtain, but also have the characteristics of mesenchymal stem cells, and have great differentiation potential, strong self-renewal ability, and can highly express stem cell characteristics after passage. There is no ethical or legal controversy. Therefore, the application of dental stem cells in regenerative medicine and tissue engineering is very promising.

[0003] When extracting dental stem cell extracts, the cultured cells need to be centrifuged, left to stand, and the supernatant liquid removed multiple times. In the prior art, the supernatant liquid is removed manually after the centrifugation, which requires the staff to access the centrifuge equipment multiple times, which is very cumbersome.

[0004] Since dental stem cells are in liquid form during culture, it is easy to think of using a small water pump or other equipment to extract the supernatant. The main difficulty lies in allowing the water pump's suction port to contact the supernatant and controlling the suction depth.

[0005] Therefore, it is necessary to provide a dental stem cell extract centrifugation device to solve the above problems. Summary of the invention

[0006] Based on the above problems existing in the prior art, the purpose of the embodiments of the present application is to provide a cell extract centrifugation device for dental stem cells to achieve the effect of extracting the supernatant.

[0007] The technical solution adopted by the present application to solve its technical problems is: a cell extract centrifuge device for dental stem cells, comprising a centrifuge table, a plurality of centrifuge frames are distributed on the circumferential side walls of the centrifuge table, a cover plate is fixed on the upper side of the centrifuge table, guide rails corresponding to the positions of the centrifuge frames are distributed on the upper side of the cover plate, a first slider and a second slider are slidably arranged on the guide rail, the second slider is located on the side of the first slider close to the center of the cover plate, a hose is fixed to the end of the second slider close to the first slider, a hose hole is provided on the first slider, the hose hole connects the end of the first slider close to the second slider with the lower end of the first slider, and the end of the hose away from the second slider extends to the lower side of the first slider through the hose hole and is fixed with a counterweight.

[0008] Furthermore, a limit block is fixedly installed on one end of the guide rail away from the center of the cover plate.

[0009] Furthermore, the hose located in the accommodating groove is provided with a plurality of support rods distributed circumferentially, the support rods are fixed to the first sliding block, and the second sliding block is provided with avoidance holes corresponding to the support rods.

[0010] Furthermore, a receiving groove is provided on a side of the first slider close to the second slider, an end of the second slider close to the first slider is located inside the receiving groove, a second stopper is fixed on the upper side of the end of the second slider close to the first slider, a first stopper is fixed on the upper side of the end of the second slider close to the inside of the receiving groove, and the first stopper is located on a side of the second stopper close to the center of the cover plate.

[0011] Furthermore, a guide rod is fixed to one end of the first sliding block close to the second sliding block, a guide hole corresponding to the support rod is provided on the second sliding block, and a reset elastic member is sleeved on the guide rod.

[0012] Furthermore, a protection plate is fixed on the upper side of the cover plate, a protection groove corresponding to the position of the guide rail is arranged on the lower side of the protection plate, and the first slider and the second slider are both located in the protection groove.

[0013] Furthermore, a limiting rod is fixed to one end of the protection groove close to the center of the cover plate, a limiting elastic member is sleeved on the limiting rod, and a limiting hole corresponding to the position of the limiting rod is provided at one end of the first sliding block close to the center of the cover plate.

[0014] Furthermore, a driving groove is provided on the upper side of the centrifuge table, a driving gear is rotatably provided in the driving groove, an extraction driver is fixedly installed in the centrifuge table, the output shaft of the extraction driver is fixed to the driving gear, the driving gear is circumferentially distributed and meshed with a passive gear corresponding to the position of the centrifuge frame, the passive gear is rotatably connected to the lower side of the cover plate, the rotating shaft of the passive gear passes through the upper side of the cover plate and a moving gear is fixedly installed thereon, a first tooth is provided on one side of the first slider close to one end of the center of the cover plate, a second tooth is provided on one side of the second slider, and the first tooth and the second tooth are both meshed with the moving gear.

[0015] Furthermore, it also includes a shell, a centrifugal groove is arranged on the upper side of the shell, the centrifugal table is rotatably arranged in the centrifugal groove, a centrifugal drive is fixed inside the shell, and the output shaft of the centrifugal drive is fixed to the centrifugal table.

[0016] Furthermore, an electric control valve and a water pump are installed on the centrifugal table, and one end of the hose and the water pump are both connected to the electric control valve.

[0017] The beneficial effects of the present invention are: By moving the first slider, before extraction, the first slider is retracted into the inner side of the cover plate, so that the upper side of the centrifuge rack is exposed, so that the staff can put the test tube on the centrifuge rack at various positions. During extraction, the first slider is extended to the outer side of the cover plate, so that the lower end of the hose hole can be aligned with the opening of the upper end of the test tube. After the first slider is extended, the second slider is brought close to the first slider, so that the hose will relax. The weight of the counterweight will stretch the hose, and the counterweight will pull one end of the hose out of the hose hole, so that one end of the hose will drop and be inserted into the test tube. Then, the culture fluid between test tubes can be transferred through equipment such as a water pump. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The accompanying drawings constituting a part of the present invention are used to provide a further understanding of the present invention. The exemplary embodiments of the present invention and their descriptions are used to explain the present invention and do not constitute an improper limitation of the present invention. In the accompanying drawings: Figure 1 This is an overall schematic diagram of a cell extract centrifugation device for dental stem cells in the present application; Figure 2 for Figure 1 Exploded diagram of the centrifugal table; Figure 3 for Figure 2 A schematic diagram of the exploded state of the first slider and the second slider; Figure 4 for Figure 2 A partial cross-sectional schematic diagram of a centrifugal table; Figure 5 for Figure 4 The enlarged schematic diagram of point A in the middle; Figure 6 for Figure 2 A schematic diagram of the working states of the first slider and the second slider; In the figure: 1. Shell; 11. Centrifugal tank; 12. Centrifugal drive; 2. Centrifugal table; 21. Centrifugal frame; 22. Driving slot; 23. Driving gear; 24. Extraction drive; 3. Cover plate; 31. Guide rail; 32. Limit block; 33. Passive gear; 34. Moving gear; 4. first slider; 41. first tooth; 42. receiving groove; 43. first stopper; 44. hose hole; 45. support rod; 46. guide rod; 47. limit hole; 5. second slider; 51. second tooth; 52. second stopper; 53. hose; 54. counterweight; 55. avoidance hole; 56. guide hole; 57. reset elastic member; 6. Protection plate; 61. Protection groove; 62. Limit rod; 63. Limit elastic member; 7. Electric control valve; 8. Water pump. DETAILED DESCRIPTION

[0019] It should be noted that, in the absence of conflict, the embodiments and features in the embodiments of the present application may be combined with each other. The present invention will be described in detail below with reference to the accompanying drawings and in combination with the embodiments.

[0020] It should be noted that, unless otherwise specified, all technical and scientific terms used in this application have the same meanings as commonly understood by ordinary technicians in the technical field to which this application belongs.

[0021] In the present invention, unless otherwise specified, the directions used, such as "up" and "down", usually refer to the directions shown in the drawings, or to the vertical, perpendicular or gravity directions; similarly, for ease of understanding and description, "left" and "right" usually refer to the left and right shown in the drawings; "inside" and "outside" refer to the inside and outside relative to the outline of each component itself, but the above-mentioned directions are not used to limit the present invention.

[0022] like Figure 1-6 As shown, the present application provides a cell extract centrifugal device for dental stem cells, including a shell 1, a centrifugal tank 11 is arranged on the upper side of the shell 1, a centrifugal table 2 is rotatably arranged at the bottom of the centrifugal tank 11, and a centrifugal drive 12 is fixed inside the shell 1. In this embodiment, the centrifugal drive 12 is a motor, and the output shaft of the centrifugal drive 12 is fixed to the centrifugal table 2.

[0023] A plurality of centrifugal racks 21 are distributed on the circumferential side wall of the centrifugal table 2. It can be understood that the centrifugal racks 21 are used to place test tubes.

[0024] A cover plate 3 is fixed on the upper side of the centrifuge table 2. A guide rail 31 corresponding to the position of the centrifuge frame 21 is distributed on the upper side of the cover plate 3. A limit block 32 is fixedly installed on one end of the guide rail 31 away from the center of the cover plate 3. A first slider 4 and a second slider 5 are slidably arranged on the guide rail 31. The second slider 5 is located on the side of the first slider 4 close to the center of the cover plate 3.

[0025] A driving groove 22 is provided on the upper side of the centrifugal table 2, and a driving gear 23 is rotatably provided in the driving groove 22. An extraction driver 24 is fixedly installed in the centrifugal table 2. In the present embodiment, the extraction driver 24 is a motor, and the output shaft of the extraction driver 24 is fixed to the driving gear 23. The driving gear 23 is circumferentially distributed with passive gears 33 corresponding to the position of the centrifugal frame 21, and the passive gear 33 is meshed with the active gear 23. The passive gear 33 is rotatably connected to the lower side of the cover plate 3. The rotating shaft of the passive gear 33 passes through the upper side of the cover plate 3, and a moving gear 34 is fixedly installed thereon. A first tooth 41 is provided on one side of the first slider 4 close to the center end of the cover plate 3, and a second tooth 51 is provided on one side of the second slider 5. Both the first tooth 41 and the second tooth 51 are meshed with the moving gear 34.

[0026] A receiving groove 42 is provided on one side of the first slider 4 close to the second slider 5, and one end of the second slider 5 close to the first slider 4 is located inside the receiving groove 42. A second stopper 52 is fixed on the upper side of one end of the second slider 5 close to the first slider 4, and a first stopper 43 is fixed on the upper side of one end of the second slider 5 in the receiving groove 42, and the first stopper 43 is located on one side of the second stopper 52 close to the center of the cover plate 3.

[0027] A hose 53 is fixed to one end of the second slider 5 close to the first slider 4, and a hose hole 44 is provided on the first slider 4. The hose hole 44 connects the end of the accommodating groove 42 away from the second slider 5 with the lower end of the first slider 4. The end of the hose 53 away from the second slider 5 extends to the lower side of the first slider 4 through the hose hole 44 and is fixed with a counterweight 54.

[0028] The hose 53 in the receiving groove 42 is provided with a plurality of support rods 45 distributed circumferentially. The support rods 45 are fixed to the first slider 4 . The second slider 5 is provided with avoidance holes 55 corresponding to the support rods 45 .

[0029] A guide rod 46 is fixed to one end of the accommodating groove 42 away from the second slider 5. A guide hole 56 corresponding to the support rod 45 is provided on the second slider 5. The guide rod 46 extends into the guide hole 56. A reset elastic member 57 is sleeved on the guide rod 46. In this embodiment, the reset elastic member 57 is a spring.

[0030] A protection plate 6 is fixed on the upper side of the cover plate 3, and a protection groove 61 corresponding to the position of the guide rail 31 is provided on the lower side of the protection plate 6. The first slider 4 and the second slider 5 are both located in the protection groove 61. A limit rod 62 is fixed to one end of the protection groove 61 close to the center of the cover plate 3, and a limit elastic member 63 is sleeved on the limit rod 62. In this embodiment, the limit elastic member 63 is a spring. A limit hole 47 corresponding to the position of the limit rod 62 is provided at one end of the first slider 4 close to the center of the cover plate 3.

[0031] An electric control valve 7 is fixedly installed in the middle of the cover plate 3 , a water pump 8 is fixed on the upper side of the protection plate 6 , and one end of the hose 53 at the center of the cover plate 3 and the water pump 8 are both connected to the electric control valve 7 .

[0032] It is understandable that a control system is provided in the housing 1 , and the control system is used to control the rotation of the centrifugal drive 12 and the extraction drive 24 , and to control the electric control valve 7 so that the extraction port and the water outlet of the water pump 8 can be connected to any hose 53 .

[0033] like Figure 5 As shown, in the initial state, the first stopper 43 and the second stopper 52 are in contact, and the resetting elastic member 57 and the limiting elastic member 63 are both in a compressed state. The second slider 5 is located at one end of the protection groove 61 close to the center of the cover plate 3. At this time, the first slider 4 and the second slider 5 are completely retracted into the protection groove 61, so that the upper side of the centrifuge frame 21 is exposed, which is convenient for the staff to place the test tubes filled with culture medium and empty test tubes on the centrifuge frame 21 at various positions.

[0034] It is understandable that the protective plate 6 is provided with a number pattern (not shown) corresponding to the position of the centrifugal rack 21, so that the staff can easily identify the number of each centrifugal rack 21. After the test tubes are placed, the staff can input the position, centrifugation time, standing time and extraction depth of each test tube through the control system. After the settings are completed, the centrifugation is started.

[0035] During centrifugation, the centrifuge driver 12 drives the centrifuge table 2 to rotate, so that the centrifuge table 2 can centrifugally rotate the test tube through the centrifuge frame 21. After centrifugation, the centrifuge driver 12 stops, and according to the set rest time, the entire device stops and waits for the rest time to end.

[0036] After the static state is completed, the extraction driver 24 is started, and the extraction driver 24 drives the moving gear 34 to rotate through the driving gear 23 and the driven gear 33. The elasticity of the limiting elastic member 63 pushes the first slider 4 to move toward the outside of the protection groove 61, so that the front end of the first tooth 41 always presses against the moving gear 34. When the moving gear 34 starts to rotate, the moving gear 34 can quickly mesh with the first tooth 41, so that the moving gear 34 passes through the first tooth 41 and drives the first slider 4 to move toward the outside of the protection groove 61 along the guide rail 31.

[0037] Since the first stopper 43 is in contact with the second stopper 52 , when the first slider 4 moves, the first stopper 43 pushes the second stopper 52 , so that the second slider 5 moves together.

[0038] Since the end of the second slider 5 close to the first slider 4 is located inside the accommodating groove 42, the positions of the first tooth 41 and the second tooth 51 will partially overlap. When the end of the first tooth 41 engages with the moving gear 34, the front end of the second tooth 51 will engage with the first tooth 41.

[0039] Since the reset elastic member 57 is in a compressed state, even if the end of the first tooth 41 is separated from the moving gear 34 , when the moving gear 34 drives the second slider 5 forward through the second tooth 51 , the first slider 4 can still continue to move forward through the reset elastic member 57 .

[0040] When the end of the first tooth 41 is separated from the moving gear 34 and the first slider 4 is blocked by the stopper 32, the lower end of the hose hole 44 will be aligned with the opening of the upper end of the test tube. At this time, as the moving gear 34 continues to rotate, the second slider 5 will overcome the elastic force of the reset elastic member 57 and insert into the receiving groove 42. At this time, due to the shortening of the distance between the second slider 5 and the hose hole 44, the hose 53 in the receiving groove 42 will relax, and the weight block 54 will pull the hose 53 out of the hose hole 44 with the gravity of the hose 53, so that the hose 53 will drop and be inserted into the test tube.

[0041] In the above process, the support rod 45 is used to support the relaxed hose 53 to prevent the hose 53 from sagging and facilitate the counterweight 54 to pull the hose 53 .

[0042] By controlling the start time of the extraction drive 24, the insertion depth of the hose 53 into the test tube can be controlled, so that the hose 53 can be inserted into the supernatant after centrifugation in the test tube containing the culture medium, and the other hoses 53 will also be inserted into the empty test tube.

[0043] When the depth reaches the set extraction depth, the extraction driver 24 stops, and the water pump 8 cooperates with the electric control valve 7 to extract the upper clear liquid in the test tube containing the culture solution and transfer it to the empty test tube.

[0044] After the supernatant is extracted, the extraction driver 24 drives the driving gear 23 to rotate in the opposite direction. At this time, the moving gear 34 first passes through the second tooth 51, and drives the second slider 5 to move along the guide rail 31 toward the inner side of the protection groove 61. Through the elasticity of the reset elastic member 57, the reset elastic member 57 pushes the first slider 4 against the limit block 32, so that the first slider 4 will not move temporarily. At this time, the second slider 5 will leave the receiving groove 42, so that the hose 53 is withdrawn.

[0045] When the first stopper 43 and the second stopper 52 come into contact, one end of the hose 53 will retract into the hose hole 44. At the same time, the second stopper 52 will pull the first stopper 43 to move the first slider 4 to the inside of the protective groove 61. As the moving gear 34 continues to rotate, the first slider 4 and the second slider 5 can be reset.

[0046] In summary, the effects of automatic centrifugation, standing and transferring of the supernatant are achieved, and by pre-setting multiple data through the control system, continuous centrifugation, standing and transferring of the supernatant can be performed by repeating the above steps, thereby facilitating the extraction of dental stem cell extracts.

[0047] Obviously, the above-described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

[0048] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular form is also intended to include the plural form. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, it indicates the presence of features, steps, operations, devices, components and / or combinations thereof.

[0049] It should be noted that the terms "first", "second", etc. in the specification and claims of the present application and the above-mentioned drawings are used to distinguish similar objects, and are not necessarily used to describe a specific order or sequence. It should be understood that the numbers used in this way can be interchanged where appropriate, so that the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein.

[0050] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. For those skilled in the art, the present invention may have various modifications and variations. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

[0051] The above is only a preferred embodiment of the present invention, and the protection scope of the present invention is not limited to the above embodiments. All technical solutions under the concept of the present invention belong to the protection scope of the present invention. It should be pointed out that for ordinary technicians in this technical field, some improvements and modifications without departing from the principle of the present invention should also be regarded as the protection scope of the present invention.

Claims

1. A cell extract centrifugation device for dental stem cells, characterized in that: The centrifugal table (2) comprises a centrifugal table (2), wherein a plurality of centrifugal racks (21) are arranged on the circumferential side wall of the centrifugal table (2), a cover plate (3) is fixed on the upper side of the centrifugal table (2), a guide rail (31) corresponding to the position of the centrifugal rack (21) is arranged on the upper side of the cover plate (3), a first slider (4) and a second slider (5) are slidably arranged on the guide rail (31), the second slider (5) is located on a side of the first slider (4) close to the center of the cover plate (3), a hose (53) is fixed to an end of the second slider (5) close to the first slider (4), a hose hole (44) is arranged on the first slider (4), the hose hole (44) connects an end of the first slider (4) close to the second slider (5) with the lower end of the first slider (4), and an end of the hose (53) away from the second slider (5) extends through the hose hole (44) to the lower side of the first slider (4) and is fixed to a counterweight (54).

2. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: A limiting block (32) is fixedly mounted on one end of the guide rail (31) away from the center of the cover plate (3).

3. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: The hose (53) located in the accommodating groove (42) has a plurality of support rods (45) distributed circumferentially, the support rods (45) are fixed to the first slider (4), and the second slider (5) is provided with avoidance holes (55) corresponding to the support rods (45).

4. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: A receiving groove (42) is provided on a side of the first slider (4) close to the second slider (5); an end of the second slider (5) close to the first slider (4) is located inside the receiving groove (42); a second stopper (52) is fixed on the upper side of the end of the second slider (5) close to the first slider (4); a first stopper (43) is fixed on the upper side of the end of the second slider (5) close to the inside of the receiving groove (42); and the first stopper (43) is located on a side of the second stopper (52) close to the center of the cover plate (3).

5. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: A guide rod (46) is fixed to one end of the first sliding block (4) close to the second sliding block (5); a guide hole (56) corresponding to the support rod (45) is provided on the second sliding block (5); and a reset elastic member (57) is sleeved on the guide rod (46).

6. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: A protection plate (6) is fixed on the upper side of the cover plate (3); a protection groove (61) corresponding to the position of the guide rail (31) is provided on the lower side of the protection plate (6); and the first sliding block (4) and the second sliding block (5) are both located in the protection groove (61).

7. The cell extract centrifugation device for dental stem cells according to claim 6, characterized in that: A limiting rod (62) is fixed to one end of the protection groove (61) close to the center of the cover plate (3), a limiting elastic member (63) is sleeved on the limiting rod (62), and a limiting hole (47) corresponding to the position of the limiting rod (62) is provided at one end of the first sliding block (4) close to the center of the cover plate (3).

8. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: A driving groove (22) is provided on the upper side of the centrifugal table (2), a driving gear (23) is rotatably provided in the driving groove (22), an extraction driver (24) is fixedly installed in the centrifugal table (2), an output shaft of the extraction driver (24) is fixed to the driving gear (23), the driving gear (23) is circumferentially distributed and meshed with a driven gear (33) corresponding to the position of the centrifugal frame (21), the driven gear (33) is rotatably connected to the lower side of the cover plate (3), the rotating shaft of the driven gear (33) passes through the upper side of the cover plate (3) and a moving gear (34) is fixedly installed thereon, a first tooth (41) is provided on one side of the first slider (4) close to the center end of the cover plate (3), a second tooth (51) is provided on one side of the second slider (5), and both the first tooth (41) and the second tooth (51) are meshed with the moving gear (34).

9. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: The invention also comprises a shell (1), wherein a centrifugal groove (11) is arranged on the upper side of the shell (1), and the centrifugal table (2) is rotatably arranged in the centrifugal groove (11). A centrifugal drive (12) is fixed inside the shell (1), and an output shaft of the centrifugal drive (12) is fixed to the centrifugal table (2).

10. The cell extract centrifugation device for dental stem cells according to claim 1, characterized in that: An electric control valve (7) and a water pump (8) are installed on the centrifugal table (2), and one end of the hose (53) and the water pump (8) are both connected to the electric control valve (7).

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