One-step microdroplet manufacturing embryo culture dish

Abstract

The invention discloses a one-step microdroplet manufacturing embryo culture dish which comprises a dish body and a plurality of microdroplet preparation grooves formed in the dish body, the micro-droplet preparation tank comprises a central liquid collecting tank and a plurality of micro-pore tanks which are arranged on the peripheral side of the central liquid collecting tank in a surrounding manner; wherein the central liquid collecting tank is higher than the micro-pore tank, and a liquid separation flow channel for overflowing is arranged between the central liquid collecting tank and the micro-pore tank. According to the invention, liquid only needs to be injected into the liquid collecting groove in the center of the dish body, and the liquid can flow into the micropore grooves in the periphery through the liquid separation flow channel; liquid with the same volume is added into the multiple micropores in one step, one-step microdroplet manufacturing is achieved by means of the height difference of the micropores, and multiple microdroplets can be manufactured by only adding the liquid once; the operation time is obviously shortened, the operation efficiency is improved, and the influence of liquid drop volume volatilization on embryo culture is reduced.

Description

Technical Field

[0001] The invention relates to the technical field of embryo culture dishes, in particular to a one-step microdroplet-based embryo culture dish. Background Art

[0002] The conventional method for human embryo culture is the microdroplet method, which is to make several microdroplets of culture solution (volume of about 20-50 microliters) in a culture dish, and culture one or several embryos in each microdroplet. Operators need to make dozens to hundreds of microdroplet culture dishes every day. Operators usually use glass Pasteur tubes without scale markings to make microdroplets. The consistency of the droplet volume is difficult to ensure, and the production process is slow. The small droplet volume is easy to evaporate, resulting in changes in osmotic pressure, which will affect the embryo culture effect.

[0003] In the prior art, patent application No. 202122490052.X provides a culture dish for assisted reproduction, including a culture dish body and a dish cover arranged on the culture dish body, a cylindrical accommodating cavity is arranged in the culture dish body, a circular egg accommodating groove is arranged in the middle of the accommodating cavity, a plurality of circular sperm accommodating grooves are arranged on the inner side of the periphery of the accommodating cavity, a communication channel is arranged between the sperm accommodating groove and the egg accommodating groove, the bottom of the egg accommodating groove is arranged to exceed the sperm accommodating groove in the height direction, so that the channel is formed into an inclined shape, wherein the channel is provided with a first blocking slope at the outlet of the sperm accommodating groove, and the channel is provided with a second blocking slope at the entrance of the egg accommodating groove. The utility model can realize the screening of high-quality sperm, thereby ensuring the acquisition of high-quality fertilized eggs.

[0004] Among the prior art, when in use, the existing embryo culture dish needs to make droplets drop by drop, and the process is slow. Taking a common culture dish (about 3.5cm in diameter) as an example, it takes about 16 seconds to make 16 droplets (4 rows and 4 columns). In addition, the droplets made by artificial Pasteur tubes are difficult to control the droplet volume, and it is difficult to make all the droplet volumes consistent. And the existing microporous culture dish has no liquid scale mark, and the existing embryo culture dish, the dish body and the dish cover are closely combined, which is not conducive to gas exchange. Summary of the invention

[0005] The purpose of the present invention is to provide a one-step microdroplet embryo culture dish to solve the above technical problems existing in the use of conventional microdroplet embryo culture dishes.

[0006] In order to solve the above technical problems, the present invention adopts the following technical solutions:

[0007] The present invention provides a one-step microdroplet embryo culture dish, comprising a dish body and a plurality of microdroplet preparation grooves arranged on the dish body;

[0008] The droplet preparation tank comprises a central liquid collecting tank and a plurality of microporous tanks arranged around the outer periphery of the central liquid collecting tank; wherein the central liquid collecting tank is higher than the microporous tank, and a liquid separation channel for overflow is arranged between the central liquid collecting tank and the microporous tank.

[0009] Furthermore, the liquid separation channel is obliquely opened between the central liquid collecting groove and the microporous groove.

[0010] Furthermore, the microporous groove includes a cylindrical groove connected to the liquid separation channel and a truncated cone groove coaxially connected to the cylindrical groove.

[0011] Furthermore, an annular scale line is arranged in the microporous groove.

[0012] Furthermore, a partition is provided between adjacent microporous grooves; the partition is an annular structure.

[0013] Furthermore, a dish cover is arranged on the dish body, and a frosted surface area is arranged on the top surface of the dish cover.

[0014] A plurality of limiting columns are symmetrically arranged on the inner wall of the dish cover.

[0015] Compared with the prior art, the beneficial technical effects of the present invention are:

[0016] 1. In the present invention, it is only necessary to add liquid into the central liquid collecting groove on the dish body, and the liquid will flow to the surrounding microporous grooves through the liquid separation channel; the same volume of liquid is added to multiple micropores in one step, and the height difference of the micropores is utilized to realize one-step microdroplet production. Only one liquid is added to realize multiple microdroplet production; the operation time is significantly shortened, the operation efficiency is improved, and the influence of the volatilization of the droplet volume on the embryo culture is reduced.

[0017] 2. Compared with the conventional method of manually making droplets one by one, the size of the droplets in the present invention is difficult to be uniform, and this structure improves the consistency of the droplet volume.

[0018] 3. The cross-section of one side of the culture dish is used to attach the chip for identifying the patient, so that the chip is attached to the dish body instead of the dish cover.

[0019] 4. The frosted design of the dish cover makes it easy to identify the patient's name in the incubator in a dim environment. The frosted part does not block the embryo, so the embryo can be observed under a microscope with the cover. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The present invention will be further described below in conjunction with the accompanying drawings.

[0021] Figure 1 A schematic top view of a one-step microdroplet embryo culture dish of the present invention;

[0022] Figure 2 This is a partial top view schematic diagram of a one-step microdroplet embryo culture dish of the present invention;

[0023] Figure 3 for Figure 2 Schematic diagram of the structural cross section;

[0024] Figure 4 This is a schematic diagram of the installation positions of the central liquid collecting tank and the microporous tank on the one-step microdroplet embryo culture dish of the present invention;

[0025] Figure 5 This is a schematic diagram of the top surface structure of the upper dish cover of the one-step microdroplet embryo culture dish of the present invention;

[0026] Figure 6 This is a schematic diagram of the internal structure of the upper dish cover of the one-step microdroplet embryo culture dish of the present invention.

[0027] Explanation of reference numerals: 100, dish body; 200, droplet preparation tank; 201, central liquid collecting tank; 202, liquid separation channel; 203, microporous tank; 204, partition; 2031, cylindrical tank; 2032, truncated cone tank; 2033, scale line; 300, dish cover; 301, frosted surface area; 302, limiting column. DETAILED DESCRIPTION

[0028] This embodiment discloses a one-step microdroplet embryo culture dish, which includes a dish body 100 and a plurality of microdroplet preparation grooves 200 formed on the dish body 100.

[0029] Specifically, the droplet preparation tank 200 includes a central liquid collecting tank 201 and a plurality of microporous tanks 203 surrounding the outer periphery of the central liquid collecting tank 201; wherein the central liquid collecting tank 201 is higher than the microporous tank 203, and a liquid separation channel 202 for overflow is opened between the central liquid collecting tank 201 and the microporous tank 203.

[0030] Among them Figure 3 As shown, the liquid separation channel 202 is obliquely opened between the central liquid collecting groove 201 and the microporous groove 203. The microporous groove 203 includes a cylindrical groove 2031 connected to the liquid separation channel 202 and a truncated cone groove 2032 coaxially connected to the cylindrical groove 2031; wherein the inner diameter of the top of the truncated cone groove 2032 is greater than the inner diameter of the bottom thereof, and the top of the truncated cone groove 2032 is connected to the bottom of the cylindrical groove 2031.

[0031] When in use, only liquid needs to be added into the central liquid collecting groove 201 on the dish body 100, and the liquid will flow to the surrounding microporous grooves 203 through the liquid separation channel 202; it takes about 2 seconds to produce 16 microdroplets. Figure 1 and Figure 2As shown, a microporous structure is utilized to achieve adding the same volume of liquid to 8 microporous wells in one step.

[0032] In this embodiment, an annular scale line 2033 is prefabricated in the microporous groove 203; specifically, a first scale line and a second scale line are prefabricated in the microporous groove 203 (in specific implementation, the scale lines can represent 20ul and 30ul respectively), wherein the first scale line is higher than the second scale line. The annular scale line is prefabricated in the microporous groove 203 for easy operation and observation.

[0033] In this embodiment, a partition 204 is installed between adjacent microporous grooves 203; the partition 204 is a ring-shaped structure; and is used to prevent the liquids from being connected due to surface tension when the liquid levels in adjacent microporous grooves 203 are too high.

[0034] like Figure 5 As shown, in this embodiment, a dish cover 300 is arranged on the dish body 100, and the top surface of the dish cover 300 is prefabricated with a frosted surface area 301, wherein the friction surface is used to make a mark or write a name.

[0035] like Figure 6 As shown, in this embodiment, four limiting posts 302 are symmetrically installed on the inner wall of the dish cover 300, wherein when the dish cover 300 is covered on the dish body 100, there is a 1mm gap between the two, the dish cover 300 is circular, and the dish body 100 is non-circular (such as Figure 1 , with a cross section), these structures significantly increase the gas exchange area.

[0036] Usage process and working principle:

[0037] Culture dish body:

[0038] The body 100 of the culture dish has a diameter of 35 mm and a height of 10 mm. There are three groups of micro-droplet preparation slots 200 in the dish, each group having 8 micro-hole slots 203. The number of micro-droplets to be prepared (8, 16 or 24) is selected according to the required number of micro-droplets.

[0039] The microporous groove 203 is used for embryo culture; the microporous groove 203 is a variable diameter structure, and the microporous groove 203 includes a cylindrical groove 2031 connected to the liquid separation channel 202, and a truncated cone groove 2032 coaxially connected to the cylindrical groove 2031; its bottom inner diameter is smaller than the upper inner diameter, which is used to limit the embryo position for easy searching.

[0040] The cylindrical groove 2031 is a hollow cylinder that is approximately elliptical. The horizontal cross section of the cylindrical groove 2031 is approximately elliptical, 4.7 mm long, 3.6 mm wide, and 2.7 mm high. The volume of the upper part of the microporous groove 203 is about 34 ul.

[0041] The upper opening of the truncated cone-shaped groove 2032 is circular with a diameter of 2 mm, the bottom surface is circular with a diameter of 1.5 mm and a depth of 1 mm, and the volume of the lower part of the microporous groove 203 is about 1.8 ul.

[0042] The central liquid collecting tank 201 is a central pool, a cylinder, 6 mm in diameter, 3 mm in depth, and a volume of about 85 ul. Eight identical columnar tanks 2031 are distributed around the central liquid collecting tank 201. About 325 ul of liquid is added to the central liquid collecting tank 201. After the central liquid collecting tank 201 (about 85 ul) is filled, the liquid will evenly flow to the lower columnar tanks 2031 (about 30 ul).

[0043] In this embodiment, a liquid separation channel 202 with a slope surface is connected between the central liquid collecting groove 201 and the cylindrical groove 2031. The upper edge of the central liquid collecting groove 201 is 1.8 mm higher than the upper edge of the cylindrical groove 2031, and the horizontal distance is 0.8 mm.

[0044] In this embodiment, a partition is installed between adjacent microporous grooves 203 to prevent adjacent liquids from mixing. In this embodiment, the cross section of the dish body 100 is used to paste a chip for identifying the patient's identity. The width of the cross section is 18 mm.

[0045] In this embodiment, the diameter of the dish cover 300 is 36 mm and the height is 5 mm. The semicircular frosted area on the outer surface of the dish cover is 32 mm wide and 9.8 mm high. It is used to write the patient's name. The frosted surface 301 helps to identify the patient's name in the incubator. In this embodiment, the frosted area on the frosted surface 301 does not block the droplets and does not affect the observation of the embryos in the droplets through the dish cover 300.

[0046] The inner surface of the dish cover 300 is provided with four protruding short columns 302 (length 1.5 mm, width 1 mm, height 1 mm) for increasing the air permeability space between the dish cover and the dish body.

[0047] The above embodiments are only descriptions of the preferred methods of the invention, and are not intended to limit the scope of the invention. Without departing from the design spirit of the invention, various modifications and improvements made to the technical solutions of the invention by ordinary technicians in this field should fall within the protection scope of the claims of the invention.

Claims

1. A one-step microdroplet embryo culture dish, characterized in that: It comprises a dish body (100) and a plurality of droplet preparation grooves (200) arranged on the dish body (100); The droplet preparation tank (200) comprises a central liquid collecting tank (201) and a plurality of microporous tanks (203) arranged around the outer periphery of the central liquid collecting tank (201); wherein the central liquid collecting tank (201) is higher than the microporous tank (203), and a liquid separation channel (202) for overflow is arranged between the central liquid collecting tank (201) and the microporous tank (203).

2. The one-step microdroplet embryo culture dish according to claim 1, characterized in that: The liquid separation channel (202) is obliquely opened between the central liquid collecting groove (201) and the microporous groove (203).

3. The one-step microdroplet embryo culture dish according to claim 2, characterized in that: The microporous groove (203) comprises a columnar groove (2031) connected to the liquid separation channel (202) and a truncated cone groove (2032) coaxially connected to the columnar groove (2031).

4. The one-step microdroplet embryo culture dish according to claim 3, characterized in that: An annular scale line (2033) is provided in the microporous groove (203).

5. The one-step microdroplet embryo culture dish according to claim 4, characterized in that: A partition plate (204) is provided between adjacent microporous grooves (203); the partition plate (204) is an annular structure.

6. The one-step microdroplet embryo culture dish according to claim 5, characterized in that: A dish cover (300) is arranged on the dish body (100), and a frosted surface area (301) is provided on the top surface of the dish cover (300).

7. The one-step microdroplet embryo culture dish according to claim 6, characterized in that: A plurality of limiting posts (302) are symmetrically arranged on the inner wall of the dish cover (300).

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