Bioreactor for stretching culture of artificial tendon

Abstract

The invention discloses a bioreactor for stretching culture of artificial tendons, and relates to the technical field of bioreactors, the bioreactor comprises an incubator, a supporting plate and a rectangular sliding groove formed in the supporting plate, one side of the incubator is fixedly provided with a rotary swing mechanism, one end of the rotary swing mechanism is connected in series with a plurality of groups of stretching culture modules, and the stretching culture modules are arranged in the rectangular sliding groove. When a strip-shaped toothed plate slides in a reciprocating mode, a push-pull rod on a culture solution balancing mechanism and a piston assembly can be driven to move in a reciprocating mode in a cylinder through a connecting rod piece, and when the piston assembly moves in the cylinder in a reciprocating mode, culture solutions at all positions in a rectangular culture dish can be sucked into the cylinder to be mixed through a liquid suction pipe fitting; then the mixed culture solution in the cylinder can be circularly discharged into each part in the rectangular culture dish through the liquid outlet pipe fitting, so that the hydrodynamic environment in the reactor can be improved, and the influence on the stretching culture effect of the artificial tendon due to non-uniform deposition and distribution of the culture solution can be effectively avoided.

Description

Technical Field

[0001] The present invention relates to the technical field of bioreactors, and particularly to a bioreactor for stretching and culturing artificial tendons. Background Art

[0002] Tendon injury is a common clinical disease of the locomotor system. Traditional treatments rely on autologous or allogeneic transplantation, but there are defects such as insufficient donors and immune rejection. Tissue-engineered artificial tendons provide a new direction for solving this problem. The core is to construct tendon tissue with biological activity and mechanical properties through in vitro culture. The existing bioreactors for stretching and culturing artificial tendons are not optimally designed and have the following deficiencies: First, it is not easy to control the clamping force during the clamping process, which is likely to damage the artificial tendon; Second, the mechanical stimulation mode is single, and it can only provide unidirectional linear stretching and cannot simulate the complex in vivo stress environment of tendons such as torsion, resulting in significant differences in cell response and natural tendons; Third, the hydrodynamic environment in the bioreactor is not good, and the culture medium is prone to deposition and uneven distribution, which will affect the stretching and culturing effect of artificial tendons. Summary of the Invention

[0003] To solve the defects existing in the prior art, the present invention provides a bioreactor for stretching and culturing artificial tendons.

[0004] To solve the above technical problems, the present invention provides the following technical solutions: A bioreactor for stretching and culturing artificial tendons includes an incubator, a pallet, and a rectangular sliding groove opened on the pallet. One side of the incubator is fixedly provided with a rotary swing mechanism. One end of the rotary swing mechanism is connected in series with a plurality of stretching and culturing modules. The stretching and culturing module includes a rectangular culture dish. An articular rotation simulation mechanism is fixedly arranged inside the rectangular culture dish. One end of the articular rotation simulation mechanism is meshed and connected with a linkage mechanism at the bottom. One end of the linkage mechanism is fixedly connected with a culture medium balancing mechanism. The bottom of the rectangular culture dish is symmetrically and fixedly provided with threaded columns, and the outer sides of the threaded columns are threadedly connected with locking sleeves.

[0005] As a preferred technical solution of the present invention, the rotary swing mechanism includes a driving motor fixedly installed outside the incubator. The end of the output shaft of the driving motor is fixedly connected with a first swing arm. One end of the first swing arm is rotationally connected with a linkage arm through a rotating shaft. One end of the linkage arm is rotationally connected with a second swing arm through a rotating shaft. The other end of the second swing arm is rotationally connected with the incubator, and a first connection kit is fixedly arranged on the outer side of the end where the second swing arm is rotationally connected with the incubator.

[0006] As a preferred technical solution of the present invention, the joint rotation simulation mechanism includes a rotating shaft, a first joint simulation component and an electric push rod, the two ends of the rotating shaft are respectively fixedly connected with a second connecting kit and a linkage gear, one end of the linkage gear is fixedly provided with a connecting plug, one end of the first joint simulation component is rotatably provided with a second joint simulation component, and the second joint simulation component is fixedly sleeved on the outside of the rotating shaft, a first slideway is opened at the bottom end of the first joint simulation component, a first artificial tendon clamping mechanism is slidably connected below the first slideway, and a second artificial tendon clamping mechanism is fixedly provided at the top of the second joint simulation component.

[0007] As a preferred technical solution of the present invention, the rotating shaft is rotatably inserted into the rectangular culture dish through a sealed bearing, and the first joint simulation part is fixedly installed on the rear side of the inner cavity of the rectangular culture dish.

[0008] As a preferred technical solution of the present invention, the electric push rod is fixedly installed on the rear side of the rectangular culture dish, and a tension sensor is provided at the connection between the electric push rod and the first artificial tendon clamping mechanism.

[0009] As a preferred technical solution of the present invention, the first artificial tendon clamping mechanism and the second artificial tendon clamping mechanism both include a rectangular clamping member and an electromagnet fixedly installed in the inner cavity of the rectangular clamping member, a sliding rod is slidably inserted on the side of the rectangular clamping member away from the electromagnet, and an iron sheet and a clamping portion are fixedly connected at both ends of the sliding rod, and a return spring is sleeved on the outer side of the sliding rod between the clamping portion and the rectangular clamping member; A protective protrusion is provided on one end surface of the clamping portion.

[0010] As a preferred technical solution of the present invention, a rectangular slot is provided in the middle of each of the first connection kit and the second connection kit, and the size of the rectangular slot matches the size of the connection plug-in block.

[0011] As a preferred technical solution of the present invention, the linkage mechanism includes a strip support platform fixedly mounted on the outside of the rectangular culture dish through a bracket and a second slideway opened on the top of the strip support platform, the top of the strip support platform is slidably connected with a strip toothed plate through the second slideway, and one end of the strip toothed plate is fixedly connected with a connecting rod; The bar-shaped tooth plate is meshingly connected with the linkage gear.

[0012] As a preferred technical solution of the present invention, the culture medium balancing mechanism includes a cylinder fixedly installed at the front end of a rectangular culture dish and a push-pull rod fixedly connected to one end of a connecting rod member. One end of the push-pull rod passing through the inner cavity of the cylinder is fixedly connected with a piston assembly. Both sides of the end of the cylinder penetrating into the interior of the rectangular culture dish are respectively connected with a liquid suction pipe fitting and a liquid discharge pipe fitting. A first one-way valve and a second one-way valve are respectively provided on the liquid suction pipe fitting and the liquid discharge pipe fitting. Support pipe fittings are equidistantly and fixedly provided on the liquid suction pipe fitting and the liquid discharge pipe fitting.

[0013] As a preferred technical solution of the present invention, the installation directions of the first one-way valve and the second one-way valve are the same. The first one-way valve enables the liquid suction pipe fitting to only suck in the culture medium and not discharge the culture medium outward. The second one-way valve enables the liquid discharge pipe fitting to only discharge the culture medium outward and not suck in the culture medium inward.

[0014] The beneficial effects of the present invention are as follows: 1. In this bioreactor, under normal circumstances, the first joint simulation member and the second joint simulation member are linearly distributed. At this time, the two ends of the artificial tendon to be cultured are respectively clamped and fixed by the first artificial tendon clamping mechanism and the second artificial tendon clamping mechanism. After clamping the artificial tendon, the first artificial tendon clamping mechanism can be pulled by an electric push rod to linearly stretch and stimulate the artificial tendon clamped and fixed between the first artificial tendon clamping mechanism and the second artificial tendon clamping mechanism. By setting a tension sensor, the magnitude of the tension can be detected, facilitating the regulation of the intensity of the stretching stimulation. By setting a rotational swing mechanism, the rotational swing mechanism can drive the joint rotation simulation mechanism inside the stretching and culturing module connected to one end thereof to reciprocally rotate and swing, simulating the joint movement of the human body. Furthermore, the complex force-bearing environment of the tendon in the human body can be simulated, multiple modes of mechanical stimulation can be applied to the artificial tendon, the stretching mode can be randomly switched according to requirements, and the difference between the artificial tendon and the natural tendon can be reduced.

[0015] 2. In this bioreactor, by setting a second connection kit and a connection plug, multiple stretching and culturing modules can be modularly spliced through the second connection kit and the connection plug. The number of stretching and culturing modules can be randomly increased or decreased according to needs, meeting different cultivation requirements. Multiple artificial tendons can be cultured simultaneously. The first artificial tendon clamping mechanism and the second artificial tendon clamping mechanism both use electromagnets to energize and adsorb iron sheets, and the iron sheets then pull the clamping part through a slide bar to magnetically clamp and fix the artificial tendon. The clamping force can be precisely controlled by changing the magnitude of the current, avoiding damage to the artificial tendon caused by excessive clamping force.

[0016] 3. The bioreactor is such that the rotation and swing mechanism drives the reciprocating rotation and swing of the joint rotation simulation mechanism inside the stretching culture module connected to one end of it. While simulating the movement of human joints, the rotating shaft rod can drive the linkage gear to rotate forward and backward. When the linkage gear rotates forward and backward, it can drive the strip-shaped tooth plate on the linkage mechanism to reciprocate. When the strip-shaped tooth plate reciprocates, it can drive the push-pull rod and piston assembly on the culture medium balancing mechanism to reciprocate inside the cylinder through the connecting rod member. When the piston assembly reciprocates inside the cylinder, it can first suck the culture medium at various places inside the rectangular culture dish into the cylinder through the liquid suction pipe fitting for mixing, and then the mixed culture medium in the cylinder can be circulated and discharged into various places inside the rectangular culture dish through the liquid discharge pipe fitting, which can improve the hydrodynamic environment inside the reactor and effectively avoid the deposition and uneven distribution of the culture medium, affecting the stretching culture effect of the artificial tendon. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] The drawings are used to provide a further understanding of the present invention and form a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention and do not constitute a limitation to the present invention. In the drawings: Figure 1 is the overall structural schematic diagram of a bioreactor for stretching culture of artificial tendon according to the present invention; Figure 2 is the first partial structural schematic diagram of the present invention; Figure 3 is the second partial structural schematic diagram of the present invention; Figure 4 is the overall structural schematic diagram of the stretching culture module in the present invention; Figure 5 is the first perspective sectional structural schematic diagram of the stretching culture module in the present invention; Figure 6 is the present invention Figure 5 the enlarged structural schematic diagram at A in; Figure 7 is the second perspective sectional structural schematic diagram of the stretching culture module in the present invention; Figure 8 is the present invention Figure 7 the enlarged structural schematic diagram at B in; Figure 9 is the sectional structural schematic diagram of the joint rotation simulation mechanism in the present invention; Figure 10 is the present invention Figure 9 the enlarged structural schematic diagram at C in.

[0018] In the figures: 1. Incubator; 2. Support plate; 3. Rectangular chute; 4. Rotary swing mechanism; 401. Driving motor; 402. First swing arm; 403. Linkage arm; 404. Second swing arm; 405. First connection kit 5. Stretching culture module 6. Rectangular culture dish 7. Joint rotation simulation mechanism; 701. Rotating shaft rod; 702. First joint simulation part; 703. Second connection kit; 704. Linkage gear; 705. Connecting plug; 706. Second joint simulation part; 707. First slideway; 708. First artificial tendon clamping mechanism; 7081. Rectangular clamping part; 7082. Electromagnet; 7083. Slide bar; 7084. Iron sheet; 7085. Clamping part; 7086. Return spring; 709. Second artificial tendon clamping mechanism; 710. Electric push rod; 711. Tensile force sensor 8. Linkage mechanism; 801. Strip-shaped support platform; 802. Strip-shaped toothed plate; 803. Connecting rod part 9. Culture medium balancing mechanism; 901. Cylinder body; 902. Push-pull rod; 903. Piston assembly; 904. Liquid suction pipe fitting; 905. Liquid outlet pipe fitting; 906. First one-way valve; 907. Second one-way valve; 908. Branch pipe fitting 10. Threaded pillar 11. Locking nut sleeve Detailed implementation mode

[0019] The following describes the preferred embodiments of the present invention with reference to the accompanying drawings. It should be understood that the preferred embodiments described herein are only used to illustrate and explain the present invention, and are not used to limit the present invention

[0020] Embodiment: As Figure 1-10As shown in the figure, a bioreactor for artificial tendon stretching culture includes an incubator 1, a pallet 2, and a rectangular chute 3 opened on the pallet 2. A rotary swing mechanism 4 is fixedly installed on one side of the incubator 1. Through the rotary swing mechanism 4, the joint rotation simulation mechanism 7 inside the stretching culture module 5 connected at one end can be driven to reciprocally rotate and swing, which can simulate the joint movement of the human body. Furthermore, it can simulate the complex stress environment of tendons in the human body, perform mechanical stimulation on artificial tendons in multiple modes, can freely switch the stretching mode according to needs, and can reduce the difference between artificial tendons and natural tendons. One end of the rotary swing mechanism 4 is connected in series with multiple groups of stretching culture modules 5. The stretching culture module 5 includes a rectangular culture dish 6. Inside the rectangular culture dish 6, a joint rotation simulation mechanism 7 is fixedly installed. One end of the bottom of the joint rotation simulation mechanism 7 is meshed and connected with a linkage mechanism 8. One end of the linkage mechanism 8 is fixedly connected with a culture medium balancing mechanism 9. Symmetrically fixed at the bottom of the rectangular culture dish 6 are threaded columns 10. The outer sides of the threaded columns 10 are threadedly connected with locking sleeves 11. Through the threaded columns 10 and the locking sleeves 11, the assembled stretching culture module 5 can be locked and fixed.

[0021] Among them, the rotary swing mechanism 4 includes a driving motor 401 fixedly installed on the outside of the incubator 1. The end of the output shaft of the driving motor 401 is fixedly connected with a first swing arm 402. One end of the first swing arm 402 is rotatably connected with a linkage arm 403 through a rotating shaft. One end of the linkage arm 403 is rotatably connected with a second swing arm 404 through a rotating shaft. The other end of the second swing arm 404 is rotatably connected with the incubator 1. Fixedly installed on the outside of the end of the second swing arm 404 rotatably connected with the incubator 1 is a first connection kit 405. When the driving motor 401 rotates, it can drive the first swing arm 402 to rotate. When the first swing arm 402 rotates, it can drive the second swing arm 404 to reciprocally rotate and swing through the linkage arm 403. When the second swing arm 404 reciprocally rotates and swings, it can drive the joint rotation simulation mechanism 7 to rotate and swing through the first connection kit 405 to simulate the joint movement of the human body.

[0022] Among them, the joint rotation simulation mechanism 7 includes a rotating shaft rod 701, a first joint simulation member 702 and an electric push rod 710. Both ends of the rotating shaft rod 701 are fixedly connected with a second connection kit 703 and a linkage gear 704 respectively. One end of the linkage gear 704 is fixedly provided with a connecting plug 705. One end of the first joint simulation member 702 is rotatably provided with a second joint simulation member 706, and the second joint simulation member 706 is fixedly sleeved outside the rotating shaft rod 701. A first slideway 707 is opened at the bottom end of the first joint simulation member 702. A first artificial tendon clamping mechanism 708 is slidably connected below the first slideway 707. A second artificial tendon clamping mechanism 709 is fixedly provided at the top end of the second joint simulation member 706. Under normal circumstances, the first joint simulation member 702 and the second joint simulation member 706 are linearly distributed. At this time, the two ends of the artificial tendon to be cultured are clamped and fixed by the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 respectively. After clamping the artificial tendon, the first artificial tendon clamping mechanism 708 is pulled by the electric push rod 710, and the artificial tendon clamped and fixed between the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 can be linearly stretched and stimulated. The magnitude of the pulling force can be detected by the pulling force sensor 711, which is convenient for regulating the intensity of the stretching stimulation. When rotational stretching stimulation of the artificial tendon is required, the rotation and swing mechanism 4 is turned on. The rotation and swing mechanism 4 can drive the rotating shaft rod 701 on the joint rotation simulation mechanism 7 connected to one end of it to rotate. When the rotating shaft rod 701 rotates, it can drive the second joint simulation member 706 to rotate and swing. When the second joint simulation member 706 rotates and swings, it can cooperate with the first joint simulation member 702 to simulate the movement of human joints. Furthermore, the complex stress environment of tendons in the human body can be simulated, various modes of mechanical stimulation can be applied to the artificial tendon, the stretching mode can be randomly switched according to requirements, and the difference between the artificial tendon and the natural tendon can be reduced.

[0023] Among them, the rotating shaft rod 701 is rotatably inserted through the rectangular culture dish 6 through a sealed bearing, and the first joint simulation member 702 is fixedly installed at the rear side of the inner cavity of the rectangular culture dish 6.

[0024] Among them, the electric push rod 710 is fixedly installed at the rear side of the rectangular culture dish 6, and a pulling force sensor 711 is provided at the connection between the electric push rod 710 and the first artificial tendon clamping mechanism 708. The artificial tendon clamped and fixed between the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 can be linearly stretched and stimulated by pulling the first artificial tendon clamping mechanism 708 by the electric push rod 710. The magnitude of the pulling force can be detected by the pulling force sensor 711, which is convenient for regulating the intensity of the stretching stimulation.

[0025] Among them, both the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 include a rectangular clamping member 7081 and an electromagnet 7082 fixedly installed in the inner cavity of the rectangular clamping member 7081. A sliding rod 7083 is slidably inserted through one side of the rectangular clamping member 7081 away from the electromagnet 7082. Iron sheets 7084 and clamping parts 7085 are respectively fixedly connected to both ends of the sliding rod 7083. A return spring 7086 is sleeved outside the sliding rod 7083 between the clamping part 7085 and the rectangular clamping member 7081. Both the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 use the electromagnet 7082 to be energized to adsorb the iron sheet 7084, and the iron sheet 7084 then pulls the clamping part 7085 through the sliding rod 7083 to magnetically clamp and fix the artificial tendon. The clamping force can be precisely controlled by changing the magnitude of the current applied to the electromagnet 7082, and damage to the artificial tendon caused by excessive clamping force can be avoided; A protective protrusion is provided on the surface of one end of the clamping part 7085, which can improve the clamping stability.

[0026] Among them, rectangular clamping grooves are provided in the middle of both the first connection kit 405 and the second connection kit 703, and the size of the rectangular clamping groove matches the size of the connection plug 705. The second connection kit 703 and the connection plug 705 can be used to modularly assemble multiple groups of stretching culture modules 5. The number of stretching culture modules 5 can be increased or decreased as needed, which can meet different cultivation requirements and can cultivate multiple groups of artificial tendons simultaneously.

[0027] Among them, the linkage mechanism 8 includes a strip-shaped support platform 801 fixedly installed outside the rectangular culture dish 6 through a bracket and a second slideway opened at the top of the strip-shaped support platform 801. A strip-shaped toothed plate 802 is slidably connected to the top of the strip-shaped support platform 801 through the second slideway. One end of the strip-shaped toothed plate 802 is fixedly connected to a connecting rod member 803. The rotating shaft rod 701 can drive the linkage gear 704 to rotate forward and backward. When the linkage gear 704 rotates forward and backward, it can drive the strip-shaped toothed plate 802 on the linkage mechanism 8 to slide reciprocally. When the strip-shaped toothed plate 802 slides reciprocally, it can drive the push rod 902 and the piston assembly 903 on the culture medium equalizing mechanism 9 to reciprocate inside the cylinder 901 through the connecting rod member 803; The strip-shaped toothed plate 802 is meshed and connected with the linkage gear 704.

[0028] Among them, the culture medium balancing mechanism 9 includes a cylinder body 901 fixedly installed at the front end of the rectangular culture dish 6 and a push-pull rod 902 fixedly connected to one end of the connecting rod member 803. One end of the push-pull rod 902 passing through the inner cavity of the cylinder body 901 is fixedly connected with a piston assembly 903. Both sides of the end of the cylinder body 901 passing through the inside of the rectangular culture dish 6 are respectively connected with a liquid suction pipe fitting 904 and a liquid discharge pipe fitting 905. A first one-way valve 906 and a second one-way valve 907 are respectively arranged on the liquid suction pipe fitting 904 and the liquid discharge pipe fitting 905. Support pipe fittings 908 are equidistantly and fixedly arranged on the liquid suction pipe fitting 904 and the liquid discharge pipe fitting 905. The rotary swing mechanism 4 drives the joint rotation simulation mechanism 7 inside the stretching culture module 5 connected to one end of it to reciprocally rotate and swing. While simulating the movement of the human joints, the rotating shaft rod 701 can drive the linkage gear 704 to rotate forward and backward. When the linkage gear 704 rotates forward and backward, it can drive the strip-shaped tooth plate 802 on the linkage mechanism 8 to reciprocally slide. When the strip-shaped tooth plate 802 reciprocally slides, it can drive the push-pull rod 902 and the piston assembly 903 on the culture medium balancing mechanism 9 to reciprocally move inside the cylinder body 901 through the connecting rod member 803. When the piston assembly 903 reciprocally moves inside the cylinder body 901, it can first suck the culture medium everywhere inside the rectangular culture dish 6 into the cylinder body 901 through the liquid suction pipe fitting 904 for mixing, and then discharge the mixed culture medium inside the cylinder body 901 into everywhere inside the rectangular culture dish 6 through the liquid discharge pipe fitting 905 in a circulating manner, which can improve the hydrodynamic environment inside the reactor and effectively avoid the uneven deposition and distribution of the culture medium, affecting the stretching culture effect of the artificial tendon.

[0029] Among them, the installation directions of the first one-way valve 906 and the second one-way valve 907 are the same. The first one-way valve 906 enables the liquid suction pipe fitting 904 to only suck in the culture medium and cannot discharge the culture medium outward. The second one-way valve 907 enables the liquid discharge pipe fitting 905 to only discharge the culture medium outward and cannot suck in the culture medium inward.

[0030] During operation, under normal circumstances, the first joint simulation part 702 and the second joint simulation part 706 are linearly distributed. At this time, the two ends of the artificial tendon to be cultured are clamped and fixed by the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 respectively. After clamping the artificial tendon, the first artificial tendon clamping mechanism 708 is pulled by the electric push rod 710 to linearly stretch and stimulate the artificial tendon clamped and fixed between the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709. The magnitude of the pulling force can be detected by the pulling force sensor 711, which is convenient for regulating the intensity of the stretching stimulation. When rotational stretching stimulation is required for the artificial tendon, the rotary swing mechanism 4 is turned on. The rotary swing mechanism 4 can drive the rotary shaft rod 701 on the joint rotation simulation mechanism 7 connected to one end of it to rotate. When the rotary shaft rod 701 rotates, it can drive the second joint simulation part 706 to rotate and swing. When the second joint simulation part 706 rotates and swings, it can cooperate with the first joint simulation part 702 to simulate the movement of human joints. Furthermore, it can simulate the complex stress environment of tendons in the human body, perform mechanical stimulation on the artificial tendon in multiple modes, and can randomly switch the stretching mode according to requirements, reducing the difference between the artificial tendon and the natural tendon; Both the first artificial tendon clamping mechanism 708 and the second artificial tendon clamping mechanism 709 use the method that the electromagnet 7082 is energized to adsorb the iron sheet 7084, and the iron sheet 7084 then pulls the clamping part 7085 through the sliding rod 7083 to magnetically clamp and fix the artificial tendon. The clamping force can be precisely controlled by changing the magnitude of the current applied to the electromagnet 7082, avoiding damage to the artificial tendon caused by excessive clamping force. The multi-group stretching and culturing modules 5 can be modularly spliced through the second connection kit 703 and the connection plug 705. The number of stretching and culturing modules 5 can be randomly increased or decreased according to needs, meeting different cultivation requirements, and multiple groups of artificial tendons can be cultured simultaneously; The rotary swing mechanism 4 drives the joint rotation simulation mechanism 7 inside the stretching and culturing module 5 connected to one end of it to reciprocally rotate and swing. While simulating the movement of human joints, the rotary shaft rod 701 can drive the linkage gear 704 to rotate forward and backward. When the linkage gear 704 rotates forward and backward, it can drive the strip-shaped tooth plate 802 on the linkage mechanism 8 to reciprocally slide. When the strip-shaped tooth plate 802 reciprocally slides, the push rod 902 and the piston assembly 903 on the culture medium equalization mechanism 9 can be driven to reciprocally move inside the cylinder 901 through the connecting rod member 803. When the piston assembly 903 reciprocally moves inside the cylinder 901, the culture medium at various parts inside the rectangular culture dish 6 can be first sucked into the cylinder 901 through the liquid suction pipe fitting 904 for mixing, and then the mixed culture medium inside the cylinder 901 can be circulated and discharged into various parts inside the rectangular culture dish 6 through the liquid outlet pipe fitting 905, improving the hydrodynamic environment inside the reactor.

[0031] Finally, it should be noted that the above are only the preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art can still modify the technical solutions recorded in the foregoing embodiments or perform equivalent replacements on some of the technical features. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. A bioreactor for stretching and culturing artificial tendons, comprising an incubator (1), a pallet (2), and a rectangular chute (3) opened on the pallet (2), characterized in that, On one side of the incubator (1), a rotary swing mechanism (4) is fixedly installed. One end of the rotary swing mechanism (4) is connected in series with multiple stretching culture modules (5). The stretching culture module (5) includes a rectangular culture dish (6). Inside the rectangular culture dish (6), a joint rotation simulation mechanism (7) is fixedly installed. One end of the bottom of the joint rotation simulation mechanism (7) is meshed and connected with a linkage mechanism (8). One end of the linkage mechanism (8) is fixedly connected with a culture solution equalization mechanism (9). Symmetrically, threaded columns (10) are fixedly installed at the bottom of the rectangular culture dish (6). The outer side of the threaded columns (10) is threadedly connected with locking sleeves (11).

2. The bioreactor for artificial tendon stretching culture according to claim 1, wherein The rotary swing mechanism (4) includes a drive motor (401) fixedly installed on the outer side of the incubator (1). The end of the output shaft of the drive motor (401) is fixedly connected with a first swing arm (402). One end of the first swing arm (402) is rotatably connected with a linkage arm (403) through a rotating shaft. One end of the linkage arm (403) is rotatably connected with a second swing arm (404) through a rotating shaft. The other end of the second swing arm (404) is rotatably connected with the incubator (1). On the outer side of the end of the second swing arm (404) rotatably connected with the incubator (1), a first connection kit (405) is fixedly installed.

3. The bioreactor for artificial tendon stretching culture according to claim 2, characterized in that, The joint rotation simulation mechanism (7) includes a rotating shaft rod (701), a first joint simulation part (702) and an electric push rod (710). The two ends of the rotating shaft rod (701) are respectively fixedly connected with a second connection kit (703) and a linkage gear (704). One end of the linkage gear (704) is fixedly provided with a connecting plug (705). One end of the first joint simulation part (702) is rotatably provided with a second joint simulation part (706), and the second joint simulation part (706) is fixedly sleeved on the outer side of the rotating shaft rod (701). A first sliding track (707) is opened at the bottom end of the first joint simulation part (702). A first artificial tendon clamping mechanism (708) is slidably connected below the first sliding track (707). A second artificial tendon clamping mechanism (709) is fixedly installed at the top end of the second joint simulation part (706).

4. A bioreactor for artificial tendon stretching culture according to claim 3, characterized in that, The rotating shaft rod (701) is rotatably inserted through the rectangular culture dish (6) through a sealed bearing. The first joint simulation part (702) is fixedly installed at the rear side of the inner cavity of the rectangular culture dish (6).

5. A bioreactor for artificial tendon stretching culture according to claim 3, characterized in that, The electric push rod (710) is fixedly installed at the rear side of the rectangular culture dish (6), and a tension sensor (711) is arranged at the connection part of the electric push rod (710) and the first artificial tendon clamping mechanism (708).

6. The bioreactor for artificial tendon stretching culture according to claim 5, wherein, Both the first artificial tendon clamping mechanism (708) and the second artificial tendon clamping mechanism (709) include a rectangular clamping member (7081) and an electromagnet (7082) fixedly installed in the inner cavity of the rectangular clamping member (7081). A slide bar (7083) is slidably inserted through one side of the rectangular clamping member (7081) away from the electromagnet (7082). Iron sheets (7084) and clamping portions (7085) are respectively fixedly connected to both ends of the slide bar (7083). A return spring (7086) is sleeved outside the slide bar (7083) between the clamping portion (7085) and the rectangular clamping member (7081). A protective protrusion is provided on the surface of one end of the clamping portion (7085).

7. A bioreactor for artificial tendon stretching culture according to claim 3, characterized in that, Rectangular clamping grooves are provided in the middle of both the first connection kit (405) and the second connection kit (703), and the sizes of the rectangular clamping grooves match the sizes of the connection inserts (705).

8. A bioreactor for artificial tendon stretching culture according to claim 3, characterized in that, The linkage mechanism (8) includes a strip-shaped support platform (801) fixedly installed outside the rectangular culture dish (6) through a bracket and a second slideway opened at the top of the strip-shaped support platform (801). A strip-shaped toothed plate (802) is slidably connected to the top of the strip-shaped support platform (801) through the second slideway. One end of the strip-shaped toothed plate (802) is fixedly connected to a connecting rod member (803). The strip-shaped toothed plate (802) is meshed with the linkage gear (704).

9. A bioreactor for artificial tendon stretching culture according to claim 8, characterized in that, The culture medium equalizing mechanism (9) includes a cylinder body (901) fixedly installed at the front end of the rectangular culture dish (6) and a push-pull rod (902) fixedly connected to one end of the connecting rod member (803). A piston assembly (903) is fixedly connected to the end of the push-pull rod (902) passing through the inner cavity of the cylinder body (901). Suction pipe fittings (904) and liquid discharge pipe fittings (905) are respectively connected to both sides of the end of the cylinder body (901) penetrating through the inside of the rectangular culture dish (6). A first one-way valve (906) and a second one-way valve (907) are respectively provided on the suction pipe fittings (904) and the liquid discharge pipe fittings (905). Support pipe fittings (908) are equidistantly and fixedly provided on the suction pipe fittings (904) and the liquid discharge pipe fittings (905).

10. A bioreactor for artificial tendon stretching culture according to claim 9, characterized in that, The installation directions of the first one-way valve (906) and the second one-way valve (907) are the same. The first one-way valve (906) enables the suction pipe fitting (904) to only suck in the culture medium and not discharge the culture medium outward. The second one-way valve (907) enables the liquid discharge pipe fitting (905) to only discharge the culture medium outward and not suck in the culture medium inward.

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