Cultivation method for increasing content of lonicera caerulea fruit polyphenol by using light-transmitting film

Abstract

The invention discloses a cultivation method for increasing the polyphenol content of lonicera caerulea fruits through a light-transmitting film, and belongs to the technical field of fruit tree cultivation. The method changes the light quality of the growth and development environment of the lonicera caerulea fruits by building a light-transmitting film greenhouse with through holes, and comprises the following steps: in the growth period of the lonicera caerulea fruits, building a greenhouse frame, covering the greenhouse frame with a blue light-transmitting film, and forming a plurality of through holes in the surface of the light-transmitting film; the light-transmitting film enables sunlight to form single blue-dominated visible light after penetrating through the surface of the light-transmitting film; the sunlight spectrum is not changed through the through holes. The quality of light irradiated to the lonicera edulis tree is changed through the blue light-transmitting film, the through holes in the light-transmitting film ensure that the spectrum of sunlight is not changed, growth and development of lonicera edulis fruits are effectively adjusted, accumulation of beneficial substance components such as phenols, flavones and anthocyanin is facilitated, the quality of the lonicera edulis fruits is improved, and compared with an existing method of manually setting a light source, the method has the advantages that the cost is low; and the method has the advantages of fast effectiveness, low cost, strong field operability and environmental friendliness.

Description

Technical Field

[0001] The invention relates to the technical field of fruit tree cultivation, and in particular to a cultivation method for increasing the polyphenol content of blue honeysuckle fruit by utilizing a light-transmitting film. Background Art

[0002] Blue indigo fruit (Lonicera caerulea L.) is a deciduous shrub of the Caprifoliaceae family and the genus Honeysuckle. The fruit is rich in polyphenolic nutrients such as phenols, flavonoids, anthocyanins, etc. It can be eaten fresh or processed into food and used in medical care. It is a "third-generation emerging small berry" with broad prospects. The European Commission also issued a regulation in December 2018 to approve the launch of blue indigo fruit as a new type of berry on the market. The polyphenol content of blue indigo fruit is one of the important indicators for measuring fruit quality, which directly affects the nutritional value and commercial value of blue indigo fruit. Usually, the polyphenol content of the fruit is increased by spraying plant hormones, adding fertilizers, etc., but it requires a large investment of manpower and material resources, and it is difficult to control the optimal application amount under field conditions.

[0003] Light is an important environmental factor for plant growth. Light quality affects the carboxylation efficiency of plants to a certain extent, thereby affecting the carbon metabolism of plants, such as photosynthetic fixation, carbohydrate transport, conversion and accumulation, etc., and further affecting the content of various components in plants. Light of different wavelengths has an important regulatory effect on plant morphology, growth and development, physiological metabolism, and nutritional quality. In agricultural production, artificial light sources are usually used to change the spectrum, but this consumes a lot of electricity and has low economic benefits.

[0004] Changing the light quality of the environment by setting a filter film above the fruit trees is low-cost, easy to operate, green and environmentally friendly, and has good economic benefits. There are no reports on the cultivation method of using a transparent film to increase the polyphenol content of blue indigo fruit. Summary of the invention

[0005] The invention aims to provide a cultivation method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film.

[0006] The present invention is achieved through the following technical solutions:

[0007] A cultivation method for increasing the polyphenol content of blue indigo fruit by using a light-transmitting film comprises the following steps: building a trellis when the blue indigo fruit turns from green to red, covering the top of the trellis with a light-transmitting film, wherein the light-transmitting film is blue and a plurality of through holes are arranged on the surface of the light-transmitting film; the light-transmitting film allows sunlight to form a single blue-dominated visible light below the light-transmitting film after passing through the surface of the light-transmitting film; and the through holes allow the sunlight spectrum to be irradiated on the blue indigo fruit tree unchanged.

[0008] In the present invention, the material of the light-transmitting film is not limited, and it can be a light-transmitting film such as plastic, knitted, or organic glass. The thickness of the light-transmitting film is not limited.

[0009] Preferably, the shed is constructed by combining steel pipes and scaffolding, and the shed is 0.3m to 1.5m higher than the top of the blue indigo fruit tree, which is conducive to ventilation and the growth of the blue indigo fruit tree.

[0010] In the present invention, the construction of the scaffold is not limited to the above-mentioned forms, and any form of scaffold construction can be applied to the present invention. The height, width and length of the scaffold can be adjusted according to the actual growth conditions of the blue indigo fruit tree.

[0011] Preferably, the light-shielding rate of the light-transmitting film is 70% to 95%, which effectively increases the polyphenol content of blue indigo fruit and provides other trace light for plant growth.

[0012] Preferably, the through holes are evenly arranged on the light-transmitting film, so that the visible light and the invisible light are evenly irradiated on the blue indigo fruit tree.

[0013] Preferably, the light-transmitting film is used during the growth and development period of the blue indigo fruit, and is recovered after the fruit is picked.

[0014] Preferably, the light-transmitting film covers the top of the scaffold for 20 to 50 days, which is selected according to actual use needs.

[0015] Preferably, the periphery of the light-transmitting film is open or closed. The choice is made according to actual use needs. Generally speaking, it is easier to operate with the light-transmitting film open.

[0016] Preferably, the light-transmitting film is a blue sunshade net, which is cheap and easy to obtain.

[0017] The beneficial effects of the present invention are:

[0018] 1. The present invention uses a transparent film as a shed film to effectively change the light quality irradiated on the blue indigo fruit trees. The through holes make visible blue light the main illumination spectrum while ensuring that the solar spectrum is unchanged. During the development period of blue indigo fruit, after a certain period of light-transmitting film coverage, the polyphenol content is significantly increased compared with open field cultivation and black shade net cultivation.

[0019] 2. The light-transmitting film used in the present invention saves more energy than artificial light sources and can be recycled for many years. The steps for setting the light-transmitting film are simple and low in cost, so it is convenient for large-scale plantations and has good economic benefits. DETAILED DESCRIPTION

[0020] The following will be combined with specific embodiments of the present invention to clearly and completely describe the technical solution of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] The following is a comparative experiment using blue shade nets, black shade nets, and no shade nets in different blue indigo fruit plantations.

[0022] Example 1

[0023] The blue indigo fruit variety selected is Smurf; three blue indigo fruit plantations of the same area are selected and numbered as A1, A2, and A3.

[0024] In the A1 plantation, a trellis was built with a height of 0.5 meters and covered with a blue sunshade net with a shading rate of 80%. The treatment started when the blue indigo fruit turned from red to green. The treatment lasted for a total of 20 days, and the blue sunshade net was spread all around.

[0025] In the A2 plantation, a trellis was built with a height of 0.5 meters and covered with a black shade net with a shading rate of 80%. The treatment started when the blue indigo fruit turned from red to green. The treatment lasted for a total of 20 days, and the black shade net was spread all around.

[0026] There was no shade net above the A3 plantation, and the treatment lasted a total of 20 days.

[0027] After the fruits were ripe, blue indigo fruit from the blue indigo fruit plantation were taken. There were 3 treatments in this experiment, 3 replicates in each treatment, and 10 blue indigo fruit in each replicate. The contents of total phenols, total flavonoids, and total anthocyanins were measured. The average values ​​of the experimental results are shown in Table 1:

[0028] Table 1 Effects of different shade net treatments on polyphenol content in blue Indigofera edulis fruit

[0029] Table 1 Smurfs Without sunshade(A3) Black sunshade net (A2) Blue sunshade net (A1) Total phenols (mg / 100g) 3944.49 4311.05 4655.11 Total flavonoids (mg / 100g) 914.97 1044.09 1146.11 Total anthocyanins (mg / 100g) 375.98 403.96 470.18

[0030] In this embodiment, after 20 days of treatment, the total phenol content of the blue sunshade net is increased by about 18% compared with the non-sunshade net; the blue sunshade net is increased by about 8% compared with the black sunshade net.

[0031] In terms of total flavonoids, the blue shade net is about 25% higher than the non-shade net; the blue shade net is about 10% higher than the black shade net.

[0032] In terms of total anthocyanins, the blue shade net is about 25% higher than the non-shade net; the blue shade net is about 16% higher than the black shade net.

[0033] Example 2

[0034] The blue indigo fruit variety selected is Beilei; three blue indigo fruit plantations of the same area are selected and numbered as A1, A2, and A3.

[0035] In the A1 plantation, a trellis was built with a height of 0.5 meters and covered with a blue sunshade net with a shading rate of 80%. The treatment started when the blue indigo fruit turned from red to green. The treatment lasted for a total of 20 days, and the blue sunshade net was spread all around.

[0036] In the A2 plantation, a trellis was built with a height of 0.5 meters and covered with a black shade net with a shading rate of 80%. The treatment started when the blue indigo fruit turned from red to green. The treatment lasted for a total of 20 days, and the black shade net was spread all around.

[0037] There was no shade net above the A3 plantation, and the treatment lasted a total of 20 days.

[0038] After the fruits were ripe, blue indigo fruit from the blue indigo fruit plantation were taken. There were 3 treatments in this experiment, 3 replicates in each treatment, and 10 blue indigo fruit in each replicate. The contents of total phenols, total flavonoids, and total anthocyanins were measured. The average values ​​of the experimental results are shown in Table 2:

[0039] Table 2 Effects of different shade net treatments on polyphenol content in blue Indigofera edulis fruit

[0040] Table 2 bud Without sunshade(A3) Black sunshade net (A2) Blue sunshade net (A1) Total phenols (mg / 100g) 3689.39 4078.2 4211.35 Total flavonoids (mg / 100g) 847.5 986.34 1069.43 Total anthocyanins (mg / 100g) 353.6 350.54 403.45

[0041] In this embodiment, after 20 days of treatment, the total phenol content of the blue sunshade net is increased by about 14% compared with the non-sunshade net; the blue sunshade net is increased by about 3% compared with the black sunshade net.

[0042] In terms of total flavonoids, the blue shade net increases by about 26% compared with the non-shade net; the blue shade net increases by about 8% compared with the black shade net.

[0043] In terms of total anthocyanins, the blue shade net is about 14% higher than the non-shade net; the blue shade net is about 15% higher than the black shade net.

[0044] Example 3

[0045] The blue indigo fruit variety selected is Ulan; three blue indigo fruit plantations of the same area are selected and numbered as A1, A2, and A3.

[0046] In the A1 plantation, a trellis was built with a height of 0.5 meters and covered with a blue sunshade net with a shading rate of 80%. The treatment started when the blue indigo fruit turned from red to green. The treatment lasted for a total of 20 days, and the blue sunshade net was spread all around.

[0047] In the A2 plantation, a trellis was built with a height of 0.5 meters and covered with a black shade net with a shading rate of 80%. The treatment started when the blue indigo fruit turned from red to green. The treatment lasted for a total of 20 days, and the black shade net was spread all around.

[0048] There was no shade net above the A3 plantation, and the treatment lasted a total of 20 days.

[0049] After the fruits were ripe, blue indigo fruit from the blue indigo fruit plantation were taken. There were 3 treatments in this experiment, 3 replicates in each treatment, and 10 blue indigo fruit in each replicate. The contents of total phenols, total flavonoids, and total anthocyanins were measured. The average values ​​of the experimental results are shown in Table 3:

[0050] Table 3 Effects of different shade net treatments on polyphenol content in blue Indigofera edulis fruit

[0051] Table 3 Ulan Without sunshade(A3) Black sunshade net (A2) Blue sunshade net (A1) Total phenols (mg / 100g) 3554.67 4034.56 4235.5 Total flavonoids (mg / 100g) 857.43 936.7 998.56 Total anthocyanins (mg / 100g) 308.65 333.21 360.5

[0052] In this embodiment, after 20 days of treatment, the total phenol content of the blue sunshade net is increased by about 19% compared with the non-sunshade net; the blue sunshade net is increased by about 5% compared with the black sunshade net.

[0053] In terms of total flavonoids, the blue shade net is about 16% higher than the non-shade net; the blue shade net is about 7% higher than the black shade net.

[0054] In terms of total anthocyanins, the blue shade net increases by about 17% compared with the non-shade net; the blue shade net increases by about 8% compared with the black shade net.

[0055] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but will conform to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film, characterized in that: The method of using a light-transmitting film to increase the polyphenol content of blue indigo fruit is to build a trellis during the development period of blue indigo fruit and cover the trellis with a light-transmitting film, wherein the light-transmitting film is blue and a plurality of through holes are arranged on the surface of the light-transmitting film; the light-transmitting film allows sunlight to form a single blue-dominated visible light below the light-transmitting film after passing through the surface of the light-transmitting film; and the through holes allow the sunlight spectrum to be irradiated on the blue indigo tree unchanged.

2. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The shed is constructed by combining steel pipes and scaffolding. The shed is 0.3m to 1.5m higher than the top of the blue indigo fruit tree, which is conducive to ventilation and the growth of the blue indigo fruit tree.

3. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The light-transmitting film is a blue sunshade net.

4. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The light-shielding rate of the light-transmitting film is 70% to 95%, which effectively improves the polyphenol content of blue indigo fruit and provides other trace light for plant growth.

5. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The through holes are evenly arranged on the light-transmitting film.

6. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The light-transmitting film is used at any stage of the development of blue honeysuckle fruit.

7. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The light-transmitting film covers the canopy surface of the blue Indigofruit fruit for 20 to 50 days.

8. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The periphery of the light-transmitting film is open.

9. The method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to claim 1, characterized in that: The periphery of the light-transmitting film is closed.

10. A method for increasing the polyphenol content of Lonicera caerulea fruit by using a light-transmitting film according to any one of claims 1 to 8, characterized in that: Used to increase the polyphenol content of blue Indigo Carica fruit.