Indoor circulating water culture system and culture method for sea grapes

Abstract

The present invention discloses an indoor circulating water culture system for sea grapes, comprising a culture container, wherein at least one culture box is arranged in the culture container, and the bottom end of the culture box is covered with an inoculation net; an illumination unit is arranged above the culture box, and the illumination unit comprises a sliding mechanism and a lighting component, and the lighting component is slidably connected to the sliding mechanism and can slide up and down relative to the sliding mechanism; a circulating water unit comprises a conveying pipeline, and a filtering device, a sterilizing device and a cooling device are sequentially connected to the conveying pipeline from the water outlet to the water inlet; a carbon dioxide supplement device; a nutrient solution supplement device and a water quality monitoring control unit. The present invention ensures the healthy growth of sea grapes in a disease-free and impurity-free environment through scientific water quality management and environmental control, improves product quality, shortens the culture cycle, and promotes the healthy development of the sea grape industry.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to an indoor circulating water culture system and a culture method for sea grapes. Background Art

[0002] Sea grapes (Long-stem grape fern algae) are widely used in food, beauty and medical fields due to their unique nutritional value and efficacy. However, these industries have high requirements for the purity of sea grapes. Marine pollution not only affects the quality of sea grapes, but may also have a significant impact on their safety. Sea grapes grown in the natural environment are often accompanied by the growth of harmful algae, which sometimes produce toxins, directly affecting the quality and safety of sea grapes, and thus adversely affecting the entire industry. In addition, sea grapes have very strict requirements for the growth environment, and changes in the environment may cause irreparable damage to their growth.

[0003] Traditional open waters are susceptible to pollutants and environmental factors are difficult to control, which affects the growth and quality of sea grapes. Therefore, traditional open waters can no longer fully guarantee their quality and safety. Based on this, in order to meet the market demand for high-purity sea grapes, it is particularly important to develop a system that can cultivate pure and disease-free sea grapes in a closed and controllable environment. This can not only improve the product quality of sea grapes and meet market demand, but also shorten the breeding cycle and improve production efficiency.

[0004] As an emerging aquaculture technology, the recirculating water aquaculture system has significant environmental control capabilities. Through the circulating water system, it can effectively isolate external pollution, maintain a stable aquaculture environment, and provide the best growth conditions for sea grapes. At the same time, the circulating water system can filter and purify water quality to prevent the growth of harmful algae, thereby ensuring the purity and safety of sea grapes. Summary of the invention

[0005] The purpose of the present invention is to provide an indoor circulating water culture system for sea grapes, which ensures the healthy growth of sea grapes in a disease-free and impurity-free environment through scientific water quality management and environmental control, improves product quality, shortens the culture cycle, and promotes the healthy development of the sea grape industry.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] An indoor circulating water culture system for sea grapes comprises a culture container, a lighting unit, a circulating water unit, a carbon dioxide supplement device, a nutrient solution supplement device and a water quality monitoring and control unit; at least one culture box is arranged in the culture container, and the bottom end of the culture box is covered with an inoculation net; the lighting unit is arranged above the culture box, and the lighting unit comprises a sliding mechanism and a lighting component, the lighting component is horizontally arranged above the culture box and forms a set distance between the culture box and the lighting component is slidably connected to the sliding mechanism and can slide up and down relative to the sliding mechanism; the circulating water unit comprises a conveying pipeline, A filtering device, a sterilizing device and a cooling device, the two ends of the delivery pipeline are respectively connected to the water inlet and the water outlet of the breeding box, and the filtering device, the sterilizing device and the cooling device are sequentially connected from the water outlet to the water inlet of the delivery pipeline; a carbon dioxide replenishing device comprises a reaction gas cylinder and a carbon dioxide attenuator connected to the gas outlet of the reaction gas cylinder, and the carbon dioxide attenuator is arranged at the lower end of the inoculation net; a nutrient solution replenishing device comprises a nutrient solution storage tank, and a flow regulating component is arranged on the nutrient solution storage tank; a water quality monitoring control unit is arranged in the breeding box and is located below the horizontal plane.

[0008] In an optional embodiment, the sliding mechanism includes two sliding rods, which are vertically connected to the top ends of two opposite sides of the breeding box, and the light assembly is slidably connected between the two sliding rods. The light assembly includes a light panel, and UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far-infrared lamp beads are arranged on the light panel. The UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far-infrared lamp beads are arranged in a ratio of 1:3:2:3:1.

[0009] In an optional embodiment, the filtering device includes a filtering water bucket, the inner wall of which is fitted with filter cotton, a plurality of coral bones are arranged in the middle of the filtering water bucket, a plurality of bacterial bricks are filled between the coral bones and the filter cotton, and the plurality of bacterial bricks are circumferentially arranged between the filter cotton and the coral bones; the sterilization device includes a sterilization box, and a plurality of ultraviolet light tubes are arranged in the sterilization box.

[0010] In an optional embodiment, the inoculation net is detachably connected to the breeding box via a buckle or a slot, and a plurality of micropores and grooves are provided on the surface of the inoculation net.

[0011] In an optional embodiment, the water quality monitoring control unit includes a pH sensor, a dissolved oxygen sensor, a temperature sensor, a salinity sensor, an ammonia nitrogen sensor and an alarm module, and the pH sensor, dissolved oxygen sensor, temperature sensor, salinity sensor, ammonia nitrogen sensor and alarm module are all electrically connected to the controller.

[0012] The present invention also provides a method for culturing sea grapes indoors by circulating water, which utilizes the sea grapes indoors by circulating water system, and comprises the following steps: S1, inoculation stage: selecting healthy algae strains that are healthy, disease-free, free of algae, and have a stem length of 4-6 cm, the water quality used in the circulating water unit is fresh artificial seawater, the pH value of the water quality is 8.0-8.2, the temperature is 23-26°C, the salinity is 30-35ppt, the dissolved oxygen is greater than 1.1mg / L, the water flow rate in the culture box is controlled to be 0.1-0.2m / s, nutrient solution is added to the water to adjust the hardness and mineral content of the water quality, and the algae strains are inoculated into the inoculation net The volume ratio of the nutrient solution added to the water is 0.001:1; S2, the initial stage of seedling growth: after inoculation, the seedlings begin to grow, and the pH value, temperature and salinity of the water body are controlled by the water quality monitoring and control unit to always remain the same as the inoculation stage, wherein the salinity is controlled by adding artificial sea salt and purified water every day, and the light intensity is controlled at 2000-2500Lux for 10-12h / day for a total of one month, and nutrient solution is added to the water every week, and the amount of nutrient solution added is the same as step S1; S3, the development stage: the temperature of the water body is raised to 26-28℃, and the salinity continues to be maintained at 30-35ppt, The pH value remains unchanged, slide the light assembly downward, and gradually increase the light intensity to 4800-5200 Lux. The illumination time is 12-15 hours / day for a total of one month. At the same time, add nutrient solution to the water every 3 days, and the amount added is the same as in the above step S1 to ensure the rapid development of the algae. During the illumination time, turn on the carbon dioxide supplement device to supplement carbon dioxide to the water body. The flow rate of carbon dioxide is 0.2-0.6g / m3 per hour; S4, mature stage: the water temperature is controlled at 26-28℃, the salinity continues to be maintained at 30-35ppt, the pH remains unchanged, and the water flow rate is increased to maintain at 6-8mg / L. Move the light assembly downward, increase the light intensity to 5800-6200 Lux, and the lighting time is 14-16h / day for a total of one month. At the same time, add nutrient solution to the water every 3 days. The amount of nutrient solution added is the same as in the above step S1. Carbon dioxide is supplemented during the lighting time, and the flow rate of carbon dioxide is the same as in step S3. S5, harvesting stage: harvest after 90-110 days of cultivation. Within 7 days before harvesting, reduce the water temperature to 22-24°C, the salinity to 28-30ppt, and the lighting time to 6-8h / day. Stop adding nutrient solution and supplying carbon dioxide, and then pick manually.

[0013] In an optional implementation, in step S2, the illumination intensity is 2000 Lux, and the illumination time is 10 h / day; in step S3, the illumination intensity is 5000 Lux, 12 h / day; in step S4, the illumination intensity is 6000 Lux, 14 h / day.

[0014] In an optional embodiment, in step S2, step S3, and step S4, the nutrient solution is added 2 hours after the lights are turned off and the dark cycle begins.

[0015] Compared with the prior art, the technical solution of the present invention has the following advantages:

[0016] 1. The present invention provides a closed and controllable breeding environment, and realizes high-quality sea grape breeding without diseases and impurities through a circulating water system, thereby reducing breeding costs. Artificial simulated seawater and strict water quality management are used to ensure the purity of the breeding water and meet the growth requirements of high-quality sea grapes.

[0017] 2. The present invention uses a filtering device and a sterilizing device to effectively remove harmful substances and pathogens in the water to ensure the healthy growth of sea grapes; through a lighting unit, a carbon dioxide supplement device and a cooling device, the optimal growth environment of sea grapes is simulated, the breeding efficiency and product quality are improved, and the artificial simulated seawater composition is accurately formulated to ensure the consistency and stability of the water composition of each batch of sea grapes cultured.

[0018] 3. The whole system is easy to operate and maintain, and can achieve efficient sea grape cultivation in a relatively short period of time, providing a scientific and effective method for industrial development and is suitable for large-scale production. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 This is a schematic diagram of the structure of the indoor circulating water culture system for sea grapes in an embodiment of the present invention;

[0020] Figure 2 is a schematic structural diagram of an illumination unit in an embodiment of the present invention;

[0021] Figure 3 This is a schematic diagram of the cross-section structure of the filter barrel in an embodiment of the present invention;

[0022] Figure 4 A diagram showing the growth of the upright stems of sea grapes cultured in an embodiment of the present invention and sea grapes cultured in a traditional mode;

[0023] Figure 5 This is a comparison chart of chlorophyll content between sea grapes cultured in an embodiment of the present invention and sea grapes cultured in a traditional mode;

[0024] Figure 6 The stem slices are of sea grapes cultured in the embodiment of the present invention and sea grapes cultured in the traditional mode;

[0025] Figure 7 This is a comparison chart of the nutrient content of sea grapes cultured in an embodiment of the present invention and sea grapes cultured in a traditional mode;

[0026] Figure 8This is a comparison chart of the active ingredients and flavor substances content of sea grapes cultured in the embodiment of the present invention and sea grapes cultured in the traditional mode;

[0027] Wherein, the accompanying drawings are marked as follows:

[0028] 1. Breeding container; 2. Breeding box; 3. Locking bolt; 4. Sliding sleeve; 5. Sliding rod; 6. Light panel; 7. Filter bucket; 71. Filter cotton; 72. Bacteria brick; 73. Coral bone; 8. Sterilization box; 9. Cooling machine; 10. Water inlet pipe; 11. Water outlet pipe; 12. Inoculation net; 13. Adjustment rod. DETAILED DESCRIPTION

[0029] In order to enable those skilled in the art to better understand the solution of the present application, the technical solution in the embodiments of the present application will be clearly and completely described below in conjunction with the drawings in the embodiments of the present application. Obviously, the described embodiments are only part of the embodiments of the present application, not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of this application.

[0030] Example 1

[0031] Sea grape indoor circulating water culture system

[0032] like Figure 1-3 As shown, this embodiment provides a sea grape indoor circulating water culture system, including a culture container 1, a lighting unit, a circulating water unit, a carbon dioxide supplement device, a nutrient solution supplement device and a water quality detection control unit; at least one culture box 2 is arranged in the culture container 1, and the bottom end of the culture box 2 is covered with an inoculation net 12; the lighting unit is arranged above the culture box 2, and the lighting unit includes a sliding mechanism and a lighting component, the lighting component is horizontally arranged above the culture box 2 and forms a set distance between the culture box 2, and the lighting component is slidably connected to the sliding mechanism and can slide up and down relative to the sliding mechanism; the circulating water unit The element includes a conveying pipeline, a filtering device, a sterilizing device and a cooling device, the two ends of the conveying pipeline are respectively connected to the water inlet and the water outlet of the breeding box, and the conveying pipeline is sequentially connected with the filtering device, the sterilizing device and the cooling device from the water outlet to the water inlet; the carbon dioxide replenishing device includes a reaction gas cylinder and a carbon dioxide attenuator connected to the gas outlet of the reaction gas cylinder, and the carbon dioxide attenuator is arranged at the lower end of the inoculation net 12; the nutrient solution replenishing device includes a nutrient solution storage tank, and the nutrient solution storage tank is provided with a flow regulating component; the water quality detection control unit is arranged in the breeding box 2 and is located below the horizontal plane.

[0033] As a further preferred solution, the sliding mechanism includes two sliding rods 5, which are vertically connected to the top ends of two opposite sides of the breeding box 2, and the light assembly is slidably connected between the two sliding rods 5. The light assembly includes a light panel 6, and UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far-infrared lamp beads are arranged on the light panel 6. The UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far-infrared lamp beads are arranged in a ratio of 1:3:2:3:1.

[0034] In the above embodiment, the breeding box 2 and the lighting unit are specifically designed as follows: there are multiple breeding boxes 2, and the multiple breeding boxes 2 are arranged side by side. The breeding box 2 is a space for the growth of sea grapes. There are water inlets, water outlets and drains, which are respectively located at the lower left end, upper right end and bottom of the breeding box 2. The length of the breeding box 2 can be set to 2m, the width is 0.5m, and the height is 0.4m. It is made of high-strength PVC material, and is equipped with filter screens at the water inlet and outlet to prevent large particles from entering the breeding box 2 and the filtering device. The diameter is 2.5 cm. A water flow control mechanism is also set on the water inlet and outlet of the breeding box 2, including a flow regulating valve to ensure that the water body flows evenly and controls the water flow rate at 0.1m / s. The flow state of the water flow in the breeding box 2 is controlled by the flow control mechanism to ensure that the water flow is in a slight flow state, which is helpful to increase the dissolved oxygen content in the water, and can also avoid the accumulation of sediments in the water, can maintain the stability of the breeding environment, and promote the healthy growth of sea grapes. The drain can be used to regularly clean and maintain the environment in the breeding box 2.

[0035] A plurality of breeding boxes 2 are connected by circulation units to realize water circulation. The inoculation net 12 is laid flat on the bottom of the breeding box 2 to ensure that the inoculation net 12 is evenly covered so that the sea grape algae segments are evenly attached to the grid. The inoculation net 12 uses food-grade PVC material and adds an epoxy resin antibacterial coating to prevent bacteria or diseases from breeding on the grid surface, thereby maintaining clean water quality and a good growth environment. The grid aperture is 3cm×3cm, which can effectively support the growth of sea grapes and ensure smooth water flow. It is connected to the lower part of the breeding box 2 through a buckle or slot, and micropores and micro grooves are added to the grid surface. The vibrator can automatically clean the grid surface regularly.

[0036] Two sliding rods 5 are vertically arranged on the tops of the two opposite surfaces of the breeding box 2. In order to increase stability, a double-rod structure can also be set. The light panel 6 covers the top of the breeding box 2 at a certain interval. The two sides of the light panel 6 are slidably connected with the sliding rods 5 through the sliding sleeves 4. The height of the light source can be manually raised and lowered. The full-spectrum algae lamps in the light panel 6 include UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far-infrared lamp beads. The lamp beads are reasonably distributed to simulate outdoor natural light. The light cycle is adjusted by an efficient LED drive power supply and a programmable controller. Among them, the sliding rod 5 is made of stainless steel, and the size of the light panel 6 is slightly smaller than the top area of ​​the breeding box 2. The panel size can be specifically 2m×0.3m, and high-strength aluminum alloy material is used.

[0037] Specifically, the sliding sleeve 4 is sleeved on the surface of the sliding rod 5 and is slidably connected to the sliding rod 5. A locking bolt 3 is passed through the sliding sleeve 4, and the sliding sleeve 4 can be fixed on the sliding rod 5 through the action of the locking bolt 3. Therefore, the height of the light panel 6 can be adjusted up and down at will. After adjustment, it can be locked and fixed by the locking bolt 3. The height of the light panel 6 can be adjusted to change the intensity of the light to adapt to algae at different growth stages. Furthermore, the height value from the breeding box can be marked on the sliding rod 5. This has a certain reference role in the process of adjusting the height of the light panel 6. The light panel 6 can be adjusted to a position of corresponding height according to the different growth stages of the algae. In order to further improve the convenience of adjustment, an adjusting rod 13 is connected between the sliding sleeves 4 on the same side. When the light panel 6 is adjusted, the two locking bolts 3 on the same side are screwed out to loosen them, and then the sliding sleeve 4 on the same side can be directly pulled down through the adjusting rod 13.

[0038] In the above embodiment, one end of the circulating water unit pipeline is the water inlet pipe 10, and the other end is the water outlet pipe 11. The water inlet of the circulating water unit water inlet pipe 10 is connected to the water outlet of the breeding box 2, and the water outlet of the circulating water unit water outlet pipe 11 is connected to the water inlet of the breeding box 2. The water in the circulating water unit comes from artificial seawater, and the pH value of the water quality is between 8.0-8.2, which is suitable for the growth of sea grapes and is conducive to their normal photosynthesis and metabolic activities. Specifically, the pure water treated by reverse osmosis (RO) filtration is then passed through artificial synthetic artificial seawater, and the conductivity of the RO filtered water is less than 10 µS / cm. After preparation, it is connected to the filtering device to filter the artificially synthesized seawater through the filtering device, sterilize it through the sterilizing device, and cool it to a suitable temperature through the cooling device before it is transported to the breeding box 2. The water transported to the breeding box 2 flows out through the water outlet and passes through the filtering device, the sterilizing device and the cooling device and is then transported to the breeding box 2. The water body circulates back and forth continuously. During the circulation of the water body, due to evaporation, the salinity of the water body will become lower after a period of time, so fresh water will be supplemented as needed to adjust the salinity of the water body, and the salinity of the water body is always kept between 30-35ppt.

[0039] In an optional embodiment, the filtering device includes a filtering water bucket 7, a filter cotton 71 is fitted on the inner wall of the filtering water bucket 7, a plurality of coral bones 73 are arranged in the middle of the filtering water bucket 7, a plurality of bacterial bricks 72 are filled between the coral bones 73 and the filter cotton 71, and a plurality of the bacterial bricks 72 are circumferentially arranged between the filter cotton 71 and the coral bones 73; the sterilization device includes a sterilization box 8, and a plurality of ultraviolet light tubes are arranged in the sterilization box 8.

[0040] In the above embodiment, the filter cotton 71 of the filter device is hand-torn cashmere surface, the volume of the filter bucket 7 is 10L, the hand-torn cashmere surface is fixed around the bucket, connected by snaps to ensure a tight fit, and the coral bone 73 is placed in the middle part of the bucket to ensure that the water flows through the coral bone for deep filtration. The hand-torn cashmere surface is cleaned regularly, and the coral bone is checked and replaced. Multiple bacterial bricks 72 are filled circumferentially between the cashmere surface and the coral bone. The multiple bacterial bricks 72 are in close contact. The water flowing out of the water outlet of the breeding box 2 enters the filter bucket 7 from the water inlet at the top of the filter bucket 7, and after being filtered by the hand-torn cashmere surface and the coral bone 73, it is discharged from the bottom outlet to the sterilization device for further sterilization.

[0041] The sterilization device includes a sterilization box 8, an ultraviolet light tube, a water inlet, a water outlet, a control system and a power module. The sterilization box 8 is a water tank. The outer shell of the water tank is a water tank made of high-strength PVC material with a size of 0.8×0.4×0.3m. There are 5 ultraviolet light tubes, each 0.4 meter long and 20W in power. The ultraviolet light tube is made of quartz glass to ensure high light transmittance and durability. The water inlet and water outlet of the water tank are located on both sides of the water tank. The control system and the power module are integrated with operating switches and indicator lights to display the working status of the equipment, and the timer sets the working time of the UV lamp. After the water is filtered by the filtering device, it enters the sterilization box 8 from the water inlet, flows through the ultraviolet light tube area, kills pathogens and microorganisms, and the sterilized water is discharged from the water outlet and flows into the cooling device for cooling.

[0042] The cooling device includes a chiller 9, which includes a shell, a cooling system, a built-in high-efficiency water pump, a temperature controller and a temperature sensor, a water inlet and a water outlet. The shell material of the chiller 9 is a high-strength, corrosion-resistant metal or plastic material. The cooling system includes an efficient and energy-saving compressor and a condenser. The evaporator adopts a coil structure, and the refrigerant uses an environmentally friendly refrigerant. The temperature controller sets and displays the water temperature to ensure the temperature control accuracy. Regularly check the compressor, condenser and evaporator, clean the condenser surface, and replace the refrigerant and water pump. The water enters the chiller from the outlet of the sterilization device, is cooled by the evaporator, and is discharged from the outlet of the chiller 9 and transported to the breeding box 2.

[0043] In the above embodiment, the carbon dioxide supplement device includes a reaction gas cylinder, a pressure gauge, a bubble counter, an electromagnetic valve and a carbon dioxide refiner. The reaction gas cylinder is made of corrosion-resistant stainless steel, has a capacity of 5 liters, and is provided with a feed port and an air outlet. The pressure gauge is installed at the air outlet of the gas cylinder, the bubble counter is made of transparent high-strength plastic, the electromagnetic valve has an operating voltage of 12V, the carbon dioxide refiner is made of high-quality ceramic or microporous glass, and the carbon dioxide refiner is arranged below the inoculation net. The pressure gauge, bubble counter and electromagnetic valve are regularly checked and the carbon dioxide refiner is replaced. Carbon dioxide is generated by the reaction of citric acid and baking soda, the pressure gauge monitors the pressure in the gas cylinder, the bubble counter controls the carbon dioxide output, the electromagnetic valve automatically controls the carbon dioxide supply time, and the carbon dioxide refiner improves the gas dissolution efficiency.

[0044] In the above embodiment, the nutrient solution replenishing device is arranged above the breeding box 2. The material of the nutrient solution storage tank is corrosion-resistant high-strength plastic with a capacity of 1 liter. After the light is turned off, the dark cycle enters and the nutrient solution is slowly released after 2 hours. The nutrient solution is prepared manually to ensure the consistency and stability of the water composition of each batch of sea grapes. The flow regulating component can be controlled by an electronic valve. The electronic control valve is a conventional commercial product. The flow rate of the nutrient solution is controlled at 2.5 ml / min and is controlled by a control system. That is, when the light is turned off for two hours, the electronic valve is controlled to release the nutrient solution and the nutrient solution in the nutrient solution storage tank is slowly dripped into the breeding box 2.

[0045] In the above embodiment, the water quality detection control unit includes a pH sensor, a dissolved oxygen sensor, a temperature sensor, a salinity sensor, an ammonia nitrogen sensor and an alarm module, and the pH sensor, dissolved oxygen sensor, temperature sensor, salinity sensor, ammonia nitrogen sensor and alarm module are all electrically connected to the controller. The data acquisition and processing module integrates the data of multiple sensors, and calculates and analyzes the water quality parameters in real time through a microprocessor. The alarm module sends an audible and visual alarm signal when the parameter exceeds the set range. The alarm module sends an audible and visual alarm signal when the parameter exceeds the set range, specifically pH is 6.0-8.0, salinity is 28-35ppt, dissolved oxygen is greater than 1.1mg / L, and temperature is 22℃-28℃. The sensor is installed near the water inlet and outlet of the aquaculture water tank, the data acquisition and processing module is installed near the water outlet of the aquaculture water tank, and the audible and visual alarm is installed in a prominent position in the aquaculture area. The water quality parameter alarm threshold is set by the microprocessor, the sensor is calibrated regularly, the equipment surface is cleaned and the battery is replaced.

[0046] Example 2

[0047] The cultivation system of the above-mentioned embodiment 1 is used to carry out indoor water circulation cultivation of sea grapes, comprising the following steps:

[0048] 1. Seedling selection and inoculation stage

[0049] Select healthy algae strains with no disease or algae and a stem length of about 5 cm. Introduce fresh artificial seawater into the circulating water unit. The pH value of the water is 8.1, the temperature is 25°C, the salinity is 33ppt, and the dissolved oxygen is greater than 1.1mg / L. Control the water flow rate in the breeding tank to 0.1m / s. Add nutrient solution to the water to adjust the hardness and mineral content of the water to ensure the balance of mineral components in the water body. Add 1mL of nutrient solution to 1L of water. Before entering the seedlings, the breeding tank must be thoroughly disinfected to eliminate pathogenic microorganisms that may exist in the breeding tank. Potassium permanganate can be used for soaking and disinfection for 12 hours to avoid the occurrence of bacteria and diseases. After that, the algae strains are inoculated onto the inoculation net.

[0050] 2. Initial stage of seedling growth (1 month)

[0051] At this stage, the sea grapes begin to grow from seaweed seedlings, and their main task is to establish a sound root system and initial photosynthesis function. Since this is the critical starting stage of the sea grape life cycle, management is particularly important, especially water quality, temperature, salinity, dissolved oxygen and other factors will directly affect the growth of sea grapes.

[0052] After inoculation, the seedlings begin to grow. The pH value, temperature and salinity of the water body are monitored and controlled by the water quality monitoring control unit to keep them the same as in the inoculation stage. The salinity is controlled by adding artificial sea salt and pure water every day. Turn on the light assembly to start illumination, adjust the height of the light assembly, control the light intensity at 2000Lux, and the illumination time is 10h / day for a total of one month. Add 1ml of nutrient solution to 1L of water every week to avoid excess nutrition or affecting the growth of plants.

[0053] 3. Developmental stage (1 month)

[0054] After entering the development stage, the stems of sea grapes begin to grow faster, gradually developing more branches, and the growth rate is significantly faster. The management focus at this stage is to promote the growth of sea grapes and improve their reproductive capacity.

[0055] After entering the development stage, the water temperature was raised to 27°C. A moderate increase in temperature helped accelerate plant growth. The salinity continued to be maintained at 33ppt, and the pH value remained unchanged. The light assembly was slid downward to gradually increase the light intensity to 5000Lux, and the lighting time was extended to 12h / day. As photosynthesis accelerated, the light intensity and time were gradually increased to promote the growth and branching of plant stems and leaves for a total of one month. At the same time, 1mL of nutrient solution was added to 1L of water every 3 days. During the lighting time, the carbon dioxide supplement device was turned on to supplement carbon dioxide into the water for photosynthesis. The flow rate of carbon dioxide was 4g / m per hour. 3 ;

[0056] 4. Maturity stage (1 month)

[0057] At this stage, the stems of the sea grapes have matured, the leaves have thickened, the plants have entered a phase of rapid proliferation, nutrient reserves have begun to accumulate, the cell differentiation process has accelerated, and some sea grapes may begin to form reproductive bodies.

[0058] The water temperature continued to be maintained at 27°C. The suitable temperature promoted the plant's work in cell differentiation and nutrient reserve accumulation. The salinity continued to be maintained at 33ppt, and the pH remained unchanged. By increasing the water flow rate, the dissolved oxygen was 7mg / L to support rapid photosynthesis and metabolic activities. The light assembly was moved downward, the light intensity was increased to 6000Lux, and the lighting time was extended to 14h / day for a total of one month. At the same time, 1mL of nutrient solution was added to 1L of water every 3 days. If the algae showed white tip phenomenon, a small amount of nutrient solution was added to promote the formation of sea grape cell wall and chloroplast, enhance the plant's disease resistance and photosynthesis efficiency, and supplement carbon dioxide at the same time during the lighting time. The flow rate of carbon dioxide was 4g / m per hour. 3 , supporting sea grapes to carry out more photosynthesis, promoting their growth and branching, and improving growth efficiency.

[0059] 5. Harvest Stage

[0060] By the time the harvest begins, the stems and leaves of sea grapes have reached their optimal growth state and are rich in nutrients and flavors.

[0061] Before harvesting, the water temperature is lowered to 23℃ to avoid excessive fluctuations in water temperature. Maintaining a stable environment is conducive to the final stage of growth of sea grapes. The salinity is reduced to 28ppt to adapt to environmental changes after the sea grapes are harvested. Within 7 days before the final harvest, the light time is reduced to 8h / day to simulate the light reduction process in the natural environment, which helps to improve the flavor and quality of the sea grapes. The addition of nutrient solution and the supply of carbon dioxide are stopped, and the natural nutrient supply in the water is relied on to avoid excessive nutrients affecting the quality of the sea grapes. Sea grapes generally become thicker at the stem and the upright stem reaches more than 8cm. At this time, special tools should be used to carefully cut them to avoid damaging the stem of the plant and ensure that the sea grapes can maintain the best quality after picking.

[0062] Example 3

[0063] The breeding method of this embodiment is the same as that of the above embodiment 2, except that the temperature, salinity, pH, intensity and time of light of the water body. In this embodiment, the temperature of the initial stage of seedling growth is 23°C, the salinity is 30ppt, the pH is 8.0, the light intensity is 2200Lux, and the light time is 12h / day; the temperature of the development stage is 26°C, the light intensity is 4800Lux, and the light time is 15h / day; the water temperature of the mature stage is 26°C, the light intensity is 5800Lux, and the light time is 16h / day. The specific steps are as follows:

[0064] 1. Seedling selection and inoculation stage

[0065] Select healthy algae strains that are disease-free, free of algae, and have a stem length of about 5 cm. Introduce fresh artificial seawater into the circulating water unit. The pH value of the water is 8, the temperature is 23°C, the salinity is 30ppt, and the dissolved oxygen is greater than 1.1mg / L. Control the water flow rate in the breeding tank to 0.1m / s. Add nutrient solution to the water to adjust the hardness and mineral content of the water to ensure the balance of mineral components in the water body. Add 1mL of nutrient solution to 1L of water. Before entering the seedlings, the breeding tank must be thoroughly disinfected to eliminate pathogenic microorganisms that may exist in the breeding tank. Potassium permanganate can be used for immersion disinfection for 12 hours to avoid the occurrence of bacteria and diseases. After that, the algae strains are inoculated onto the inoculation net.

[0066] 2. Initial stage of seedling growth (1 month)

[0067] At this stage, the sea grapes begin to grow from seaweed seedlings, and their main task is to establish a sound root system and initial photosynthesis function. Since this is the critical starting stage of the sea grape life cycle, management is particularly important, especially water quality, temperature, salinity, dissolved oxygen and other factors will directly affect the growth of sea grapes.

[0068] After inoculation, the seedlings begin to grow. The pH value, temperature and salinity of the water are monitored and controlled by the water quality monitoring control unit to keep them the same as in the inoculation stage. The salinity is controlled by adding artificial sea salt and pure water every day. Turn on the light assembly to start illumination, adjust the height of the light assembly, control the light intensity at 2200Lux, and the illumination time is 12h / day for a total of one month. Add 1ml of nutrient solution to 1L of water every week to avoid overnutrition or affecting the growth of plants.

[0069] 3. Developmental stage (1 month)

[0070] After entering the development stage, the stems of sea grapes begin to grow faster, gradually developing more branches, and the growth rate is significantly faster. The management focus at this stage is to promote the growth of sea grapes and improve their reproductive capacity.

[0071] After entering the development stage, the water temperature was raised to 26°C. A moderate increase in temperature helped accelerate plant growth. The salinity continued to be maintained at 30ppt, and the pH value remained unchanged. The light assembly was slid downward to gradually increase the light intensity to 4800Lux, and the lighting time was extended to 15h / day. As photosynthesis accelerated, the light intensity and time were gradually increased to promote the growth and branching of plant stems and leaves for a total of one month. At the same time, 1mL of nutrient solution was added to 1L of water every 3 days. During the lighting time, the carbon dioxide supplement device was turned on to supplement carbon dioxide into the water for photosynthesis. The flow rate of carbon dioxide was 4g / m per hour. 3 ;

[0072] 4. Maturity stage (1 month)

[0073] At this stage, the stems of the sea grapes have matured, the leaves have thickened, the plants have entered a phase of rapid proliferation, nutrient reserves have begun to accumulate, the cell differentiation process has accelerated, and some sea grapes may begin to form reproductive bodies.

[0074] The water temperature continued to be maintained at 26℃. The suitable temperature promotes the plant's work in cell differentiation and accumulation of nutrient reserves. The salinity continued to be maintained at 30ppt, and the pH remained unchanged. The dissolved oxygen was 7mg / L by increasing the water flow rate to support rapid photosynthesis and metabolic activities. The lighting component was moved downward, the light intensity was increased to 5800Lux, and the lighting time was extended to 16h / day for a total of one month. At the same time, 1mL of nutrient solution was added to 1L of water every 3 days. If the algae showed white tips, a small amount of nutrient solution was added to promote the formation of sea grape cell walls and chloroplasts, enhance the plant's disease resistance and photosynthesis efficiency, and supplement carbon dioxide at the same time during the lighting time. The carbon dioxide flow rate was 4g / m3 per hour, which supported more photosynthesis in sea grapes, promoted their growth and branching, and improved growth efficiency.

[0075] 5. Harvest Stage

[0076] By the time the harvest begins, the stems and leaves of sea grapes have reached their optimal growth state and are rich in nutrients and flavors.

[0077] Before harvesting, the water temperature is lowered to 23℃ to avoid excessive fluctuations in water temperature. Maintaining a stable environment is conducive to the final stage of growth of sea grapes. The salinity is reduced to 28ppt to adapt to environmental changes after the sea grapes are harvested. Within 7 days before the final harvest, the light time is reduced to 8h / day to simulate the light reduction process in the natural environment, which helps to improve the flavor and quality of the sea grapes. The addition of nutrient solution and the supply of carbon dioxide are stopped, and the natural nutrient supply in the water is relied on to avoid excessive nutrients affecting the quality of the sea grapes. Sea grapes generally become thicker at the stem and the upright stem reaches more than 8cm. At this time, special tools should be used to carefully cut them to avoid damaging the stem of the plant and ensure that the sea grapes can maintain the best quality after picking.

[0078] Example 4

[0079] The breeding method of this embodiment is the same as that of the above-mentioned embodiment 2, except that the temperature, salinity, pH, intensity and time of light of the water body. In this embodiment, the temperature in the initial stage of seedling growth is 26°C, the salinity is 35ppt, the pH is 8.2, the light intensity is 2500Lux, and the light time is 11h / day; the temperature in the development stage is 28°C, the light intensity is 5200Lux, and the light time is 14h / day; the water temperature in the mature stage is 28°C, the light intensity is 6200Lux, and the light time is 15h / day.

[0080] 1. Seedling selection and inoculation stage

[0081] Select healthy algae strains with no disease or algae and a stem length of about 5 cm. Introduce fresh artificial seawater into the circulating water unit. The pH value of the water is 8.2, the temperature is 26°C, the salinity is 35ppt, and the dissolved oxygen is greater than 1.1mg / L. Control the water flow rate in the breeding tank to 0.1m / s. Add nutrient solution to the water to adjust the hardness and mineral content of the water to ensure the balance of mineral components in the water body. Add 1mL of nutrient solution to 1L of water. Before entering the seedlings, the breeding tank must be thoroughly disinfected to eliminate pathogenic microorganisms that may exist in the breeding tank. Potassium permanganate can be used for immersion disinfection for 12 hours to avoid the occurrence of bacteria and diseases. After that, the algae strains are inoculated onto the inoculation net.

[0082] 2. Initial stage of seedling growth (1 month)

[0083] At this stage, the sea grapes begin to grow from seaweed seedlings, and their main task is to establish a sound root system and initial photosynthesis function. Since this is the critical starting stage of the sea grape life cycle, management is particularly important, especially water quality, temperature, salinity, dissolved oxygen and other factors will directly affect the growth of sea grapes.

[0084] After inoculation, the seedlings begin to grow. The pH value, temperature and salinity of the water are monitored and controlled by the water quality monitoring control unit to keep them the same as in the inoculation stage. The salinity is controlled by adding artificial sea salt and pure water every day. Turn on the light assembly to start illumination, adjust the height of the light assembly, control the light intensity at 2500Lux, and the illumination time is 11h / day for a total of one month. Add 1ml of nutrient solution to 1L of water every week to avoid overnutrition or affecting the growth of plants.

[0085] 3. Developmental stage (1 month)

[0086] After entering the development stage, the stems of sea grapes begin to grow faster, gradually developing more branches, and the growth rate is significantly faster. The management focus at this stage is to promote the growth of sea grapes and improve their reproductive capacity.

[0087] After entering the development stage, the water temperature was raised to 28°C. A moderate increase in temperature helped accelerate plant growth. The salinity continued to be maintained at 35ppt, and the pH value remained unchanged. The light assembly was slid downward to gradually increase the light intensity to 5200Lux, and the lighting time was extended to 14h / day. As photosynthesis accelerated, the light intensity and time were gradually increased to promote the growth and branching of the plant's stems and leaves for a total of one month. At the same time, 1mL of nutrient solution was added to 1L of water every 3 days. During the lighting time, the carbon dioxide supplement device was turned on to supplement carbon dioxide into the water for photosynthesis. The flow rate of carbon dioxide was 4g / m3 per hour.

[0088] 4. Maturity stage (1 month)

[0089] At this stage, the stems of the sea grapes have matured, the leaves have thickened, the plants have entered a phase of rapid proliferation, nutrient reserves have begun to accumulate, the cell differentiation process has accelerated, and some sea grapes may begin to form reproductive bodies.

[0090] The water temperature continued to be maintained at 28°C. The suitable temperature promotes the plant's work in cell differentiation and accumulation of nutrient reserves. The salinity continued to be maintained at 35ppt, and the pH remained unchanged. The dissolved oxygen was 7mg / L by increasing the water flow rate to support rapid photosynthesis and metabolic activities. The lighting component was moved downward, the light intensity was increased to 6200Lux, and the lighting time was extended to 15h / day for a total of one month. At the same time, 1mL of nutrient solution was added to 1L of water every 3 days. If the algae showed white tips, a small amount of nutrient solution was added to promote the formation of sea grape cell walls and chloroplasts, enhance the plant's disease resistance and photosynthesis efficiency, and supplement carbon dioxide at the same time during the lighting time. The carbon dioxide flow rate was 4g / m3 per hour, which supported more photosynthesis in sea grapes, promoted their growth and branching, and improved growth efficiency.

[0091] 5. Harvest Stage

[0092] By the time the harvest begins, the stems and leaves of sea grapes have reached their optimal growth state and are rich in nutrients and flavors.

[0093] Before harvesting, the water temperature is lowered to 23℃ to avoid excessive fluctuations in water temperature. Maintaining a stable environment is conducive to the final stage of growth of sea grapes. The salinity is reduced to 28ppt to adapt to environmental changes after the sea grapes are harvested. Within 7 days before the final harvest, the light time is reduced to 8h / day to simulate the light reduction process in the natural environment, which helps to improve the flavor and quality of the sea grapes. The addition of nutrient solution and the supply of carbon dioxide are stopped, and the natural nutrient supply in the water is relied on to avoid excessive nutrients affecting the quality of the sea grapes. Sea grapes generally become thicker at the stem and the upright stem reaches more than 8cm. At this time, special tools should be used to carefully cut them to avoid damaging the stem of the plant and ensure that the sea grapes can maintain the best quality after picking.

[0094] Comparative Example 1

[0095] The traditional outdoor pool seawater aquaculture model is used for breeding.

[0096] Effect verification

[0097] 1. Growth and color comparison

[0098] The sea grapes cultured using the culture method of Example 2 were compared with those cultured using traditional outdoor pool seawater. Ten sea grape upright stems under the two culture modes were randomly selected and photographed for observation. The comparison results are shown in Figure 2. Figure 4 0.1 g of sea grape upright stems were randomly selected from the two cultivation modes, and the photosynthetic pigment content was determined using the ethanol colorimetric method. The results are shown in Figure 5 shown.

[0099] in, Figure 4 In Figure B, there are comparison diagrams of the growth of the upright stems of sea grapes cultured using the culture method of Example 2 (left picture) and sea grapes cultured using traditional outdoor pool seawater (right picture), Figure 4 Figures A and C are detailed comparison diagrams of the growth of the upright stems of sea grapes cultured using the culture method of Example 2 and sea grapes cultured using traditional outdoor pool seawater.

[0100] Figure 5 Figure D is a comparison chart of the chlorophyll A content of sea grapes cultured by the culture method of Example 2 and sea grapes cultured by traditional outdoor pool seawater; Figure E is a comparison chart of the chlorophyll B content of sea grapes cultured by the culture method of Example 2 and sea grapes cultured by traditional outdoor pool seawater; Figure F is a comparison chart of the total chlorophyll content of sea grapes cultured by the culture method of Example 2 and sea grapes cultured by traditional outdoor pool seawater.

[0101] from Figure 4 and Figure 5It can be seen that the sea grapes cultured by the culture method of the present invention have thicker upright stems, brighter green color, significantly higher content of photosynthetic pigments, and no algae compared to the sea grapes cultured by traditional outdoor pool seawater.

[0102] 2. Comparison of tissue cell structure

[0103] The tissue cell structure of the sea grapes cultured by the culture method of Example 2 was compared with that of the sea grapes cultured by traditional outdoor pool seawater. The sea grape runners under the two culture modes were selected for plant tissue sectioning and microscopic observation of the cell tissue structure. The results are as follows: Figure 6 shown. Figure 6 Figure A shows the cell structure of sea grape tissue in the traditional mode. Figure 6 Figure B shows the cell structure of sea grape tissue in the cultivation mode of the embodiment. Figure 6 It can be seen that the sea grape cells in the culture mode of the embodiment are arranged more closely, indicating that they are healthier and stronger in structure.

[0104] 3. Comparison of Nutritional Components

[0105] The nutritional components of sea grapes cultured by the culture method of Example 3 were compared with those cultured by traditional outdoor pool seawater. Sea grape samples cultured by the traditional culture mode and the culture mode of the embodiment were randomly selected to measure the total sugar content (measured by anthrone method), protein content (measured by Coomassie brilliant blue method) and fat content (measured by Soxhlet extraction method). The results are as follows: Figure 7 As shown in Figure AC in the figure, the results show that the total sugar content and protein content of the sea grapes cultured in the culture mode of the embodiment group are significantly higher than those of the conventional group, indicating that its nutritional value is higher.

[0106] 4. Comparison of active ingredients and flavor substances

[0107] The active ingredients and flavor substances of the sea grapes cultured by the culture method of Example 4 were compared with those of the sea grapes cultured by traditional outdoor pool seawater. The sea grape samples cultured by the traditional culture mode and the culture mode of the embodiment were randomly selected to measure the crude polysaccharide content (measured by phenol-sulfuric acid method), polyphenol content (measured by Folin phenol reagent method), flavonoid content (measured by aluminum salt colorimetry), dimethyl sulfide (measured by bromine method) and free amino acid content (measured by ninhydrin colorimetry). The measurement results are as follows: Figure 8 As shown in the DG diagram in the figure, the results show that the crude polysaccharide content, polyphenol content, flavonoid content, dimethyl sulfide and free amino acid content of the sea grapes cultivated in the cultivation mode of the embodiment are significantly higher than those in the traditional mode, indicating that the active ingredients are richer and the flavor substances are better.

[0108] Although the present invention has been described using the above preferred embodiments, it is not intended to limit the scope of protection of the present invention. Any person skilled in the art who makes various changes and modifications to the above embodiments without departing from the spirit and scope of the present invention still fall within the scope of protection of the present invention.

Claims

1. An indoor circulating water culture system for sea grapes, characterized in that: include: A breeding container, wherein at least one breeding box is arranged in the breeding container, and the bottom end of the breeding box is covered with an inoculation net; An illumination unit is arranged above the breeding box, the illumination unit comprises a sliding mechanism and a light assembly, the light assembly is horizontally arranged above the breeding box and forms a set distance between the breeding box, the light assembly is slidably connected to the sliding mechanism and can slide up and down relative to the sliding mechanism; wherein the sliding mechanism comprises two sliding rods, the two sliding rods are vertically connected to the tops of two opposite sides of the breeding box, the light assembly is slidably connected between the two sliding rods, the light assembly comprises a light panel, UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far infrared lamp beads are arranged on the light panel, and the UV lamp beads, blue light lamp beads, green light lamp beads, red light lamp beads and far infrared lamp beads are arranged in a ratio of 1:3:2:3:1; The circulating water unit comprises a conveying pipeline, a filtering device, a sterilizing device and a cooling device, wherein the two ends of the conveying pipeline are respectively connected to the water inlet and the water outlet of the breeding box, and the conveying pipeline is sequentially connected with the filtering device, the sterilizing device and the cooling device from the water outlet to the water inlet; The filtering device comprises a filtering water bucket, the inner wall of which is fitted with filter cotton, a plurality of coral bones are arranged in the middle of the filtering water bucket, a plurality of bacterial bricks are filled between the coral bones and the filter cotton, and a plurality of bacterial bricks are circumferentially arranged between the filter cotton and the coral bones; the sterilizing device comprises a sterilizing box, a plurality of ultraviolet light tubes are arranged in the sterilizing box; A carbon dioxide supplement device, comprising a reaction gas cylinder and a carbon dioxide attenuator connected to the gas outlet of the reaction gas cylinder, wherein the carbon dioxide attenuator is arranged at the lower end of the inoculation net; A nutrient solution replenishing device comprises a nutrient solution storage tank, wherein the nutrient solution storage tank is provided with a flow regulating component; A water quality monitoring control unit is arranged in the breeding box and is located below the horizontal plane.

2. The sea grape indoor circulating water culture system according to claim 1, characterized in that: The inoculation net is detachably connected to the breeding box via a buckle or a slot, and a plurality of micropores and grooves are provided on the surface of the inoculation net.

3. The sea grape indoor circulating water culture system according to claim 1, characterized in that: The water quality monitoring control unit comprises a pH sensor, a dissolved oxygen sensor, a temperature sensor, a salinity sensor, an ammonia nitrogen sensor and an alarm module, and the pH sensor, the dissolved oxygen sensor, the temperature sensor, the salinity sensor, the ammonia nitrogen sensor and the alarm module are all electrically connected to the controller.

4. A method for indoor circulating water culture of sea grapes, using the indoor circulating water culture system of sea grapes as claimed in any one of claims 1 to 3, characterized in that: The following steps are involved: S1, inoculation stage: select healthy algae strains with no disease, no algae, and a stem length of 4-6 cm. The water quality used in the circulating water unit is fresh artificial seawater, the pH value of the water quality is 8.0-8.2, the temperature is 23-26℃, the salinity is 30-35ppt, and the dissolved oxygen is greater than 1.1mg / L. The water flow rate in the breeding tank is controlled to be 0.1-0.2m / s. Nutrient solution is added to the water to adjust the hardness and mineral content of the water quality, and the algae strains are inoculated on the inoculation net, where 1mL of nutrient solution is added to 1L of water; S2, initial stage of seedling growth: after inoculation, the seedlings begin to grow, and the pH value, temperature and salinity of the water body are controlled by the water quality monitoring and control unit to always remain the same as in the inoculation stage, wherein the salinity is controlled by adding artificial sea salt and purified water every day, and the light intensity is controlled at 2000 Lux for 10 h / day for a total of one month, and nutrient solution is added to the water every week, and the amount of nutrient solution added is the same as step S1, and the time of adding the nutrient solution is 2 hours after the light is turned off and the dark cycle begins every day; S3, development stage: raise the water temperature to 26-28℃, maintain salinity at 30-35ppt, keep pH unchanged, slide the light assembly downwards, gradually increase the light intensity to 5000Lux, and light duration is 12h / day for a total of one month. At the same time, add nutrient solution to the water every 3 days, the amount added is the same as in step S1 above, to ensure rapid algae development. The nutrient solution is added 2 hours after the light is turned off every day and the dark cycle begins. During the light duration, turn on the carbon dioxide supplement device to supplement carbon dioxide to the water, and the flow rate of carbon dioxide is 0.2-0.6g / m3 per hour. S4, mature stage: the water temperature is controlled at 26-28℃, the salinity is maintained at 30-35ppt, the pH remains unchanged, the water flow rate is increased to keep the dissolved oxygen at 6-8mg / L, the light assembly is moved downward, the light intensity is increased to 6000Lux, the light time is 14h / day, a total of one month, and nutrient solution is added to the water every 3 days. The amount of nutrient solution added is the same as in step S1 above. The time for adding the nutrient solution is 2 hours after the light is turned off every day and the dark cycle begins. Carbon dioxide is added during the light time, and the flow rate of carbon dioxide is the same as in step S3; S5. Harvest stage: Harvest after 90-110 days of cultivation. Within 7 days before harvest, reduce the water temperature to 22-24℃, the salinity to 28-30ppt, the light time to 6-8h / day, stop adding nutrient solution and supplying carbon dioxide, and then pick manually.

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