Cell acquisition equipment

Abstract

The invention relates to the technical field of cell acquisition, in particular to cell acquisition equipment which comprises a needle cylinder, a first piston, a first piston rod and a needle head, the first piston is movably mounted in an inner cavity of the needle cylinder, the first piston rod is fixedly mounted in the middle of the upper surface of the first piston, and the needle head is clamped to the bottom of the needle cylinder. The cell acquisition equipment comprises a needle head, a sealing assembly is arranged on the needle head and comprises a sealing module and a cleaning module, the sealing module and the cleaning module are matched with each other to improve the cell acquisition efficiency, the sealing module comprises a steel ring fixedly mounted at the top of the needle head, and a sealing ring is fixedly mounted on the upper surface of the steel ring. The sealing ring and the needle cylinder can be tighter through the sealing assembly, so that sealing is achieved, the situation that cells leak from the connecting position of the needle head and the needle cylinder when the cells are obtained through negative pressure is avoided, the leakage-proof assembly is matched, the cells can be prevented from leaking when the negative pressure is released, and the cells are obtained more smoothly.

Description

Technical Field

[0001] The present invention relates to the technical field of cell acquisition, in particular to a cell acquisition device. Background Art

[0002] During the animal diagnosis and treatment process, when treating animals with tumors, a cytological examination is usually performed first to determine the nature of the cyst and to assist the doctor in making a judgment. During the cytological examination, fine needle aspiration is first performed to obtain cells.

[0003] When obtaining cells, the tumor is usually punctured and then the cells are obtained by using the negative pressure of the syringe. At this time, the cells will enter the needle under the action of the negative pressure, and then the needle and the syringe are separated to release the negative pressure to prevent the negative pressure from entering the syringe and making it difficult to transfer to the slide. After the negative pressure is released, the cells are pressed onto the slide for observation. However, after the negative pressure is released, the obtained cells and tissue fluid are easy to leak from the needle under the action of their own gravity, and when the needle and the syringe are separated, it is also easy for the cells to be contaminated to other places and pollute the working environment. Summary of the invention

[0004] In view of the deficiencies of the prior art, the present invention provides a cell acquisition device to solve the problems raised in the above background technology.

[0005] To achieve the above objectives, the present invention is implemented through the following technical solutions: a cell acquisition device, comprising a syringe, a first piston, a first plug rod and a needle, wherein the first piston is movably installed in the inner cavity of the syringe, the first plug rod is fixedly installed in the middle of the upper surface of the first piston, the needle is clamped at the bottom of the syringe, and a sealing assembly is arranged on the needle, wherein the sealing assembly comprises a sealing module and a cleaning module, and the sealing module and the cleaning module cooperate with each other to improve the efficiency of cell acquisition; Wherein, the sealing module comprises a steel ring fixedly mounted on the top of the needle, a sealing ring is fixedly mounted on the upper surface of the steel ring, and a wedge is fixedly mounted on the bottom of the syringe; The cleaning module comprises a disinfecting cotton fixedly mounted on the upper surface of the first piston, and a cleaning member is fixedly mounted on the upper surface of the disinfecting cotton; The needle head is provided with a leak-proof component, and the leak-proof component is used to improve the efficiency of cell acquisition.

[0006] Optionally, the anti-leakage component includes a first fixing member fixedly mounted on the needle, a receiving member is fixedly mounted on one end of the first fixing member away from the needle, the interior of the receiving member is hollow, and a first telescopic spring is fixedly mounted inside the receiving member, a telescopic member is fixedly connected to the top of the first telescopic spring, and the telescopic member is movably mounted inside the receiving member; A second fixing member is fixedly installed on one side of the telescopic member, and an end of the second fixing member away from the telescopic member is rotatably connected to a leakage-proof member via a torsion spring; A driving sleeve is fixedly installed on one side of the syringe, a self-locking knob is installed on the surface of the driving sleeve, a gear is fixedly installed on the rotating shaft of the self-locking knob, a rack is meshed on one side of the gear, a clip is fixedly installed on the bottom of the rack, the clip is clamped on the outside of the needle, and is located between the telescopic part and the steel ring.

[0007] Optionally, the syringe is provided with a liquid outlet assembly, the liquid outlet assembly comprises an arc-shaped cavity, the arc-shaped cavity is provided in the outer wall of one side of the syringe, a second piston is movably installed in the arc-shaped cavity, a second plug rod is fixedly installed on the upper surface of the second piston, and the second plug rod is movably installed between the arc-shaped cavity; A clearance groove is provided on the surface of the syringe, a catheter is fixedly installed inside the arc cavity, a positioning clamp is fixedly installed on the surface of the syringe, and one end of the catheter away from the arc cavity is located inside the positioning clamp.

[0008] Optionally, a fixing assembly is provided on the syringe, and the fixing assembly includes two adhesive pieces, the adhesive pieces are bonded to the surface of the syringe, and the side of the adhesive piece away from the syringe is rotatably connected to a fixing block through a torsion spring, and a support column is fixedly installed at the bottom of the fixing block; A limit block is fixedly installed at the bottom of the support column, a second telescopic spring is sleeved on the outer peripheral side of the support column, and a fixed foot is movably installed on the outer surface of the support column.

[0009] Optionally, the diameter of the limiting block is greater than the diameter of the supporting column, the top of the second telescopic spring is fixedly connected to the fixing block, and the bottom is fixedly connected to the fixing foot.

[0010] Optionally, the descending stroke of the telescopic member is equal to the distance from the top of the leak-proof member to the bottom of the needle, and a moisture-absorbing cloth is attached to a side of the leak-proof member close to the needle.

[0011] Optionally, the gear is located in the middle of the rack, and half of the length of the rack is greater than the descending stroke of the telescopic member.

[0012] Optionally, a positioning bolt is threadedly connected to the surface of the storage member, and the positioning bolt penetrates the surface of the storage member and extends to a hollow portion inside the storage member.

[0013] The present invention provides a cell acquisition device, which has the following beneficial effects: 1. The cell acquisition device can make the sealing ring and the syringe tighter through the sealing component, thereby achieving sealing, avoiding leakage of cells from the connection between the needle and the syringe when the cells are acquired under negative pressure, and cooperate with the leak-proof component to prevent cell leakage when the negative pressure is released, making cell acquisition smoother; 2. The cell acquisition device can avoid the problem of cell damage when spreading cells on a glass slide through the liquid outlet component, and can fix the tumor when puncturing a small or flat tumor through the fixing component, thereby further improving the smoothness of cell acquisition. BRIEF DESCRIPTION OF THE DRAWINGS

[0014] Figure 1 The structure of the present invention is shown in FIG. Figure 1 ; Figure 2 The structure of the present invention is shown in FIG. Figure 2 ; Figure 3 The structure of the present invention is shown in FIG. Figure 3 ; Figure 4 The schematic diagram of the positional relationship between the sealing component and the anti-leakage component of the present invention is shown in FIG. Figure 1 ; Figure 5 The schematic diagram of the positional relationship between the sealing component and the anti-leakage component of the present invention is shown in FIG. Figure 2 ; Figure 6 The schematic diagram of the positional relationship between the sealing component and the anti-leakage component of the present invention is shown in FIG. Figure 3 ; Figure 7 For the present invention Figure 6 A schematic diagram of the structure with a partial enlargement at the center; Figure 8 It is a schematic structural diagram of the fixing assembly of the present invention.

[0015] In the figure: 1. syringe; 11. first piston; 12. first plug rod; 13. needle; 2. steel ring; 21. sealing ring; 22. wedge-shaped piece; 23. disinfection cotton; 24. cleaning piece; 3. first fixing piece; 31. storage piece; 32. first telescopic spring; 33. telescopic piece; 34. second fixing piece; 35. leak-proof piece; 36. positioning bolt; 37. driving sleeve; 38. self-locking knob; 39. gear; 310. rack; 311. clamp; 4. arc cavity; 41. second piston; 42. second plug rod; 43. make way groove; 44. catheter; 45. positioning clamp; 5. adhesive piece; 51. fixing block; 52. support column; 53. limit block; 54. second telescopic spring; 55. fixing foot. DETAILED DESCRIPTION

[0016] The technical solutions in the embodiments of the present invention will be described clearly and completely below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0017] Example 1: Please refer to Figures 1 to 7 The present invention provides a technical solution: a cell acquisition device, comprising a syringe 1, a first piston 11, a first plug rod 12 and a needle 13, wherein the first piston 11 is movably mounted in the inner cavity of the syringe 1, the first plug rod 12 is fixedly mounted in the middle of the upper surface of the first piston 11, the needle 13 is clamped at the bottom of the syringe 1, and a sealing assembly is arranged on the needle 13, the sealing assembly comprises a sealing module and a cleaning module, and the sealing module and the cleaning module cooperate with each other to improve the efficiency of cell acquisition; The sealing module includes a steel ring 2 fixedly mounted on the top of the needle 13, a sealing ring 21 is fixedly mounted on the upper surface of the steel ring 2, and a wedge 22 is fixedly mounted on the bottom of the syringe 1; The cleaning module includes a sterilizing cotton 23 fixedly mounted on the upper surface of the first piston 11, and a cleaning member 24 is fixedly mounted on the upper surface of the sterilizing cotton 23; The needle 13 is provided with a leak-proof component, which is used to improve the efficiency of cell acquisition.

[0018] Specifically, when using, the user first needs to select the size of the needle 13 according to the size of the tumor suffered by the animal. The telescopic member 33 and the storage member 31 on the needles 13 of different sizes are equally enlarged and reduced in size. Similarly, the distance between the leak-proof member 35 and the bottom of the needle tip of the needle 13 is also different for the needles 13 of different sizes. At this time, when the needles 13 of different sizes are selected according to the size of the tumor, it can be ensured that the distance between the needle 13 and the leak-proof member 35 is sufficient to puncture the tumor, and the travel limit of the leak-proof member 35 can avoid the problem of excessive puncture, and can assist the staff to observe the puncture depth, grasp the puncture distance, and improve the puncture effect. After the size of the needle 13 is determined, the needle 13 needs to be fixed to the syringe 1. When the needle 13 is installed on the syringe 1, the sealing ring 21 will approach and contact the wedge 22. As the sealing ring 21 and the wedge 22 contact each other, the wedge 22 will squeeze the sealing ring 21, so that the sealing ring 21 and the syringe 1 are more tightly connected, thereby achieving sealing and preventing cells from leaking from the connection between the needle 13 and the syringe 1 when obtaining cells under negative pressure; After the installation is completed, it is necessary to pull the first stopper rod 12 to the top of the inner cavity of the syringe 1, and then inject disinfectant on the disinfectant cotton 23, and then repeat pulling the first stopper rod 12 two or three times. During the upward pulling of the first stopper rod 12, due to the effect of pressure, the disinfectant cotton 23 and the cleaning piece 24 will be tightly attached to the inner cavity wall of the syringe 1, so that the cleaning piece 24 cleans the inner cavity wall of the syringe 1 during the upward movement, and the disinfectant cotton 23 disinfects the inner cavity wall of the syringe 1 during the upward movement, thereby avoiding contamination in the syringe 1 when it is reused, affecting the cytological examination, wherein the disinfectant cotton 23 and the cleaning piece 24 are both detachable for easy replacement.

[0019] Example 2: Please refer to Figure 3 , Figure 4 , Figure 5 and Figure 7 The anti-leakage component includes a first fixing member 3 fixedly mounted on the needle 13, a receiving member 31 is fixedly mounted on one end of the first fixing member 3 away from the needle 13, the interior of the receiving member 31 is hollow, and a first telescopic spring 32 is fixedly mounted inside, a telescopic member 33 is fixedly connected to the top of the first telescopic spring 32, and the telescopic member 33 is movably mounted inside the receiving member 31; A second fixing member 34 is fixedly installed on one side of the telescopic member 33, and an end of the second fixing member 34 away from the telescopic member 33 is rotatably connected to a leak-proof member 35 via a torsion spring; A drive sleeve 37 is fixedly installed on one side of the syringe 1, a self-locking knob 38 is installed on the surface of the drive sleeve 37, a gear 39 is fixedly installed on the rotating shaft of the self-locking knob 38, a rack 310 is meshed on one side of the gear 39, a clamp 311 is fixedly installed on the bottom of the rack 310, the clamp 311 is clamped on the outside of the needle 13, and is located between the telescopic member 33 and the steel ring 2; The descending stroke of the telescopic member 33 is equal to the distance between the top of the leak-proof member 35 and the bottom of the needle 13. A moisture-absorbing cloth is attached to the side of the leak-proof member 35 close to the needle 13. The gear 39 is located in the middle of the rack 310, and half of the length of the rack 310 is greater than the descending stroke of the telescopic member 33; The surface of the receiving member 31 is threadedly connected with a positioning bolt 36, which penetrates the surface of the receiving member 31 and extends to the hollow inside the receiving member 31; The syringe 1 is provided with a liquid outlet assembly, which includes an arc-shaped cavity 4, which is provided in the outer wall of one side of the syringe 1, in which a second piston 41 is movably installed, and a second plug rod 42 is fixedly installed on the upper surface of the second piston 41, and the second plug rod 42 is movably installed between the arc-shaped cavity 4; A recess 43 is formed on the surface of the syringe 1 , a catheter 44 is fixedly mounted inside the arc cavity 4 , and a positioning clamp 45 is fixedly mounted on the surface of the syringe 1 , and one end of the catheter 44 away from the arc cavity 4 is located inside the positioning clamp 45 .

[0020] On the basis of Example 1, after the disinfection operation is completed, the tumor needs to be punctured. After the puncture is completed, the first plug rod 12 is pulled slightly to obtain cells by using negative pressure. After repeated extraction two to four times, the needle 13 is pulled out and the puncture site is hemostatic. At this time, the extracted cells are in the needle 13. Then, the self-locking knob 38 needs to be rotated to drive the gear 39 to rotate, so that the gear 39 drives the rack 310, so that the rack 310 moves downward; When the rack 310 moves downward, it will drive the clamp 311 to move downward. At this time, the clamp 311 will squeeze the telescopic member 33, so that the telescopic member 33 squeezes the first telescopic spring 32 and descends. The descent of the telescopic member 33 will drive the second fixing member 34 to descend, so that the leak-proof member 35 moves to the bottom of the needle tip of the needle 13. At this time, there is no interference from the needle 13. Under the reset action of the torsion spring, the leak-proof member 35 will fit with the inclined bottom of the needle tip of the needle 13 and block it. Next, the positioning bolt 36 needs to be tightened so that the positioning bolt 36 is tightly connected to the telescopic member 33. The friction between the positioning bolt 36 and the telescopic member 33 can be used to position the telescopic member 33. Then, the self-locking knob 38 is rotated continuously to drive the clamp 311 to descend, so that the telescopic member 33 drives the needle 13 to descend, thereby separating from the syringe 1 and releasing the negative pressure. Under the blocking of the leak-proof member 35, after the negative pressure is released, it is possible to prevent cells and tissue fluid from dripping and affecting the amount of cells obtained. When the negative pressure is released, the first plug rod 12 needs to be pulled upward, and then the self-locking knob 38 is rotated to drive the needle 13 to reset, so that the needle 13 and the syringe 1 are in contact, and then the second plug rod 42 is pulled, and the catheter 44 is used to suck the buffer into the arc cavity 4, and then the second plug rod 42 is pressed to drip the buffer onto the surface of the slide, and then the first plug rod 12 is pressed to introduce the cells in the needle 13 into the buffer on the slide, and then the cells are spread out. Compared with the traditional method of directly spreading the cells, the use of buffer can avoid the problem of cell breakage and improve the effect of cytological examination.

[0021] Example 3: Please refer to Figure 1 and Figure 8 The syringe 1 is provided with a fixing assembly, which includes two adhesive members 5, which are bonded to the surface of the syringe 1, and the side of the adhesive member 5 away from the syringe 1 is rotatably connected to a fixing block 51 through a torsion spring, and a support column 52 is fixedly installed at the bottom of the fixing block 51; A limit block 53 is fixedly installed at the bottom of the support column 52, a second telescopic spring 54 is sleeved on the outer peripheral side of the support column 52, and a fixed foot 55 is movably installed on the outer surface of the support column 52; The diameter of the limiting block 53 is greater than the diameter of the supporting column 52 . The top of the second telescopic spring 54 is fixedly connected to the fixing block 51 , and the bottom is fixedly connected to the fixing foot 55 .

[0022] In Example 2, when performing puncture, if a small or flat tumor is encountered, it is difficult to accurately control the puncture. The traditional method uses a non-negative pressure method to obtain cells, that is, to obtain cells using capillary action, but it is easy to cause insufficient cell quantity, or require assistants to fix the tumor to help the puncture. When there is a lack of sufficient human resources, this embodiment is selected for operation according to the actual situation; That is, a double-sided tape is pasted on one side of the adhesive member 5, and then the two adhesive members 5 are fixed on both sides of the syringe 1, and then the fixing feet 55 are unfolded so that the two fixing feet 55 are on both sides of the tumor. Under the action of the torsion spring, the fixing feet 55 will fix the tumor, thereby replacing manual fixation. It should be noted that after fixation, the bottom of the needle tip of the needle 13 is close to the tumor, and there is a gap; After the fixation is completed, puncture is performed. During the puncture, the fixing foot 55 will squeeze the second telescopic spring 54 to help the tumor to be exposed, thereby providing a stroke for the puncture, so that the puncture is completed smoothly.

[0023] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A cell acquisition device, comprising a syringe (1), a first piston (11), a first plug rod (12) and a needle (13), wherein the first piston (11) is movably mounted in the inner cavity of the syringe (1), the first plug rod (12) is fixedly mounted in the middle of the upper surface of the first piston (11), and the needle (13) is clamped at the bottom of the syringe (1), characterized in that: The needle (13) is provided with a sealing assembly, the sealing assembly comprising a sealing module and a cleaning module, the sealing module and the cleaning module cooperate with each other to improve the efficiency of cell acquisition; The sealing module comprises a steel ring (2) fixedly mounted on the top of the needle (13), a sealing ring (21) fixedly mounted on the upper surface of the steel ring (2), and a wedge-shaped piece (22) fixedly mounted on the bottom of the syringe (1); The cleaning module comprises a sterilizing cotton (23) fixedly mounted on the upper surface of the first piston (11), and a cleaning member (24) is fixedly mounted on the upper surface of the sterilizing cotton (23); The needle (13) is provided with a leak-proof component, and the leak-proof component is used to improve the efficiency of cell acquisition.

2. A cell acquisition device according to claim 1, characterized in that: The anti-leakage component comprises a first fixing member (3) fixedly mounted on the needle (13); a storage member (31) is fixedly mounted on one end of the first fixing member (3) away from the needle (13); the interior of the storage member (31) is hollow and a first telescopic spring (32) is fixedly mounted therein; a telescopic member (33) is fixedly connected to the top of the first telescopic spring (32); and the telescopic member (33) is movably mounted inside the storage member (31); A second fixing member (34) is fixedly mounted on one side of the telescopic member (33); an end of the second fixing member (34) away from the telescopic member (33) is rotatably connected to a leakage prevention member (35) via a torsion spring; A drive sleeve (37) is fixedly mounted on one side of the syringe (1); a self-locking knob (38) is mounted on the surface of the drive sleeve (37); a gear (39) is fixedly mounted on the rotating shaft of the self-locking knob (38); a rack (310) is meshed on one side of the gear (39); a clamp (311) is fixedly mounted on the bottom of the rack (310); the clamp (311) is clamped on the outside of the needle (13) and is located between the telescopic member (33) and the steel ring (2).

3. A cell acquisition device according to claim 2, characterized in that: The syringe (1) is provided with a liquid outlet assembly, the liquid outlet assembly comprising an arc-shaped cavity (4), the arc-shaped cavity (4) being provided in an outer wall of one side of the syringe (1), a second piston (41) being movably mounted in the arc-shaped cavity (4), a second plug rod (42) being fixedly mounted on the upper surface of the second piston (41), and the second plug rod (42) being movably mounted between the arc-shaped cavity (4); A clearance groove (43) is provided on the surface of the syringe (1), a catheter (44) is fixedly installed inside the arc-shaped cavity (4), and a positioning clamp (45) is fixedly installed on the surface of the syringe (1), and an end of the catheter (44) away from the arc-shaped cavity (4) is located inside the positioning clamp (45).

4. A cell acquisition device according to claim 3, characterized in that: The syringe (1) is provided with a fixing assembly, the fixing assembly comprising an adhesive piece (5), and the number of the adhesive pieces (5) is two, the adhesive piece (5) is adhered to the surface of the syringe (1), the side of the adhesive piece (5) away from the syringe (1) is rotatably connected to a fixing block (51) via a torsion spring, and a support column (52) is fixedly mounted on the bottom of the fixing block (51); A limit block (53) is fixedly mounted on the bottom of the support column (52), a second telescopic spring (54) is sleeved on the outer peripheral side of the support column (52), and a fixed foot (55) is movably mounted on the outer surface of the support column (52).

5. A cell acquisition device according to claim 4, characterized in that: The diameter of the limiting block (53) is greater than the diameter of the supporting column (52); the top of the second telescopic spring (54) is fixedly connected to the fixing block (51), and the bottom is fixedly connected to the fixing foot (55).

6. A cell acquisition device according to claim 5, characterized in that: The descending stroke of the telescopic member (33) is equal to the distance between the top of the leak-proof member (35) and the bottom of the needle (13), and a moisture-absorbing cloth is attached to the side of the leak-proof member (35) close to the needle (13).

7. A cell acquisition device according to claim 6, characterized in that: The gear (39) is located in the middle of the rack (310), and one-half of the length of the rack (310) is greater than the descending stroke of the telescopic member (33).

8. A cell acquisition device according to claim 7, characterized in that: A positioning bolt (36) is threadedly connected to the surface of the storage piece (31); the positioning bolt (36) penetrates the surface of the storage piece (31) and extends to a hollow portion inside the storage piece (31).

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