Alcohol-free washing-free antibacterial hand washing gel and preparation method thereof

Abstract

The invention discloses an alcohol-free washing-free bacteriostatic hand washing gel and a preparation method thereof. The alcohol-free washing-free bacteriostatic hand washing gel is prepared from the following components in percentage by weight: 0.05%-0.1% of chitosan quaternary ammonium salt, 0.03%-0.05% of polyhexamethylene biguanide hydrochloride, 0.1%-0.3% of didecyl dimethyl ammonium chloride, 0.1%-2.0% of a gel matrix, 0.1%-25% of a humectant and the balance of purified water. The product has good sterilizing and moisturizing effects.

Description

Technical Field

[0001] The invention relates to the technical field of toiletries, in particular to an alcohol-free, wash-free, antibacterial hand washing gel and a preparation method thereof.

[0002] Background

[0003] In our daily life and work, hands are the parts of the human body that have the most contact with the outside world and are the easiest to get dirty. On the one hand, hands will inevitably come into contact with objects containing microorganisms or even pathogenic microorganisms. On the other hand, due to the current social environment, people are more and more likely to be infected with germs through their hands when they go out on public transportation or arrive at certain public places. In particular, the number of direct and indirect germ infections caused by hands of medical workers in their daily work remains high all year round. Therefore, keeping hands clean and hygienic is of great significance to preventing diseases and protecting physical health. Among them, hand-free gel products are widely used in various occasions because they are convenient and quick to use.

[0004] Currently, the hand sanitizers on the market mainly use ethanol as the active ingredient for disinfection. The ethanol content is generally greater than 50%, which is flammable and has greatly limited its scope of use. In addition, long-term and frequent use of alcohol-containing disinfectants will disrupt the growth balance of human hand skin, causing the skin to lose elasticity and luster, and cause the skin to become tight, dry, dehydrated, and even rough, cracked, and other problems. Therefore, the development of alcohol-free formula hand sanitizers has broad application prospects and market demand. Summary of the invention

[0005] Aiming at the deficiencies of the existing wash-free antibacterial gel product technology, the present invention provides an alcohol-free wash-free antibacterial hand washing gel and a preparation method thereof.

[0006] To achieve the above object, the present invention provides the following technical solutions:

[0007] The invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is characterized by comprising a chitosan quaternary ammonium salt, polyhexamethylene biguanide hydrochloride and didecyl dimethyl ammonium chloride composition with a mass percentage concentration not greater than 0.6% of the total amount of gel raw materials.

[0008] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0009] The amount of the polyhexamethylene biguanide hydrochloride used is less than the amount of the chitosan quaternary ammonium salt and didecyl dimethyl ammonium chloride used.

[0010] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0011] The dosage of the chitosan quaternary ammonium salt is 1-5 times that of the polyhexamethylene biguanide hydrochloride.

[0012] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0013] The dosage of the didecyl dimethyl ammonium chloride is 4-6 times the dosage of the polysaccharide quaternary ammonium salt.

[0014] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0015] It also contains a gel matrix;

[0016] The amount of the gel matrix is ​​not less than 1% of the total weight of the raw materials.

[0017] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0018] The gel matrix is ​​selected from at least one of sodium carboxymethyl cellulose, methyl cellulose and hydroxypropyl methyl cellulose.

[0019] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0020] It also contains moisturizers;

[0021] The amount of the moisturizing agent is not less than 1% of the total weight of the raw materials.

[0022] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized by:

[0023] The moisturizing agent is selected from at least one of trehalose, hyaluronic acid and polyol.

[0024] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized in that it is prepared from the following components in percentage by weight:

[0025]

[0026] Furthermore, the present invention provides an alcohol-free, wash-free, antibacterial hand washing gel, which is also characterized in that it comprises the following steps:

[0027] S1. Add part of the humectant to purified water and stir to completely dissolve it to obtain solution A;

[0028] S2. Add the remaining moisturizer to solution A and mix well. Slowly add the gel matrix and chitosan quaternary ammonium salt while stirring. Stir rapidly for 10-15 minutes to make them disperse evenly. Gradually heat to 50-60°C and continue stirring to obtain a uniform and transparent gel B.

[0029] S3. Add polyhexamethylene biguanide hydrochloride and didecyl dimethyl ammonium chloride to gel B, stir well to mix, and let stand to defoam to obtain a uniform, transparent, alcohol-free, wash-free, antibacterial hand washing gel.

[0030] The present invention discloses the following technical effects:

[0031] 1. The wash-free hand gel designed by the present invention contains polyhexamethylene biguanide hydrochloride (PHMG), didecyl dimethyl ammonium chloride (DDAC) and chitosan quaternary ammonium salt (HACC), all of which are polycationic compounds that can adsorb negatively charged bacteria and aggregate on the cell wall, producing a chamber barrier effect, resulting in bacterial growth inhibition and death; at the same time, it can change the permeability of the membrane, destroy the cell structure, and cause cell dissolution and death. Moreover, the three produce a synergistic effect, further improving the bactericidal performance of the hand gel. It is particularly worth noting that a good bactericidal effect can be achieved under the premise of a low addition amount.

[0032] 2. The hand washing gel of the present invention does not contain ethanol in its solution and is non-irritating to the skin. It also solves the problem that ethanol cannot be carried in special occasions (such as airplanes) due to its flammability, strong odor and other problems.

[0033] 3. The present invention improves the moisturizing effect by adding moisturizing elements such as trehalose or hyaluronic acid, so that the skin will not feel dry after use, and it can efficiently absorb moisture to make the skin soft, smooth and shiny.

[0034] 4. In addition, in the present invention, the water-soluble chitosan quaternary ammonium salt has a moisture absorption and moisture retention performance comparable to or even better than that of hyaluronic acid. When the degree of substitution is greater than 43%, the chitosan quaternary ammonium salt has a better moisture absorption performance than hyaluronic acid, while its moisture retention performance is substantially the same as that of hyaluronic acid. At the same time, trehalose and hyaluronic acid have high moisture retention, which can reduce the damage to the skin accordingly. In other words, the presence of the chitosan quaternary ammonium salt component not only plays a synergistic antibacterial effect but also has the effect of improving moisture retention performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0035] Figure 1 Antibacterial effect test results. Specific implementation methods

[0037] The technical solution of the present invention is further described below.

[0038] Embodiment 1. An alcohol-free, wash-free, antibacterial hand washing gel, characterized in that:

[0039] The invention is prepared from the following components by weight percentage: 1.0% trehalose, 0.05% hyaluronic acid, 0.05% chitosan quaternary ammonium salt, 5.0% glycerol, 10.0% propylene glycol, 0.03% polyhexamethylene biguanide hydrochloride, 0.3% didecyldimethylammonium chloride, 1.5% hydroxypropyl methylcellulose, and the balance is purified water.

[0040] The preparation method of the above-mentioned alcohol-free, wash-free, antibacterial hand washing gel comprises the following steps:

[0041] (1) Take the required amount of purified water, add the weighed trehalose and hyaluronic acid, stir until completely dissolved, and obtain solution A;

[0042] (2) Add glycerol and propylene glycol to solution A in the required amount and mix well. Slowly add chitosan quaternary ammonium salt under stirring, then stir rapidly for 10 minutes to make it evenly dispersed. Gradually heat to 55℃±5℃, continue stirring under heating to obtain a uniform and transparent gel B, and stop heating.

[0043] (3) Based on the above gel B, add polyhexamethylene biguanide hydrochloride and didecyl dimethyl ammonium chloride in required amounts, stir and mix thoroughly, and let stand to defoam to obtain a uniform, transparent, alcohol-free, wash-free, antibacterial hand washing gel.

[0044] Embodiment 2. An alcohol-free, wash-free, antibacterial hand washing gel, characterized in that:

[0045] The invention is prepared from the following components by weight percentage: 2.0% trehalose, 0.1% chitosan quaternary ammonium salt, 10.0% propylene glycol, 5.0% glycerol, 0.03% polyhexamethylene biguanide hydrochloride, 0.1% didecyldimethylammonium chloride, 1.0% hydroxypropyl methylcellulose, and the balance is purified water.

[0046] The preparation method of the alcohol-free, wash-free, antibacterial hand washing gel has the same steps as those in Example 1, and the gel is allowed to stand and defoam to obtain a uniform and transparent alcohol-free, wash-free, antibacterial hand washing gel.

[0047] Embodiment 3. An alcohol-free, wash-free, antibacterial hand washing gel, characterized in that:

[0048] The invention is prepared from the following components by weight percentage: 1.0% trehalose, 0.05% chitosan quaternary ammonium salt, 5.0% glycerol, 10.0% propylene glycol, 0.01% polyhexamethylene biguanide hydrochloride, 0.2% didecyldimethylammonium chloride, 2.0% hydroxypropyl methylcellulose, and the balance is purified water.

[0049] The preparation method of the alcohol-free, wash-free, antibacterial hand washing gel has the same steps as those in Example 1, and the gel is allowed to stand and defoam to obtain a uniform and transparent alcohol-free, wash-free, antibacterial hand washing gel.

[0050] Comparative Example 1. An alcohol-free, wash-free, antibacterial hand washing gel, prepared from the following components by weight percentage:

[0051] Trehalose 1.0%, hyaluronic acid 0.5%, chitosan quaternary ammonium salt 0.05%, glycerol 5.0%, propylene glycol 10.0%, polyhexamethylene biguanide hydrochloride 0.05%, didecyldimethylammonium chloride 0.5%, hydroxypropyl methylcellulose 1.0%, and the balance is purified water.

[0052] The preparation method of the above-mentioned alcohol-free, wash-free, antibacterial hand washing gel has the same steps as implementation case 1, and no uniform and transparent alcohol-free, wash-free, antibacterial hand washing gel is obtained by standing and defoaming.

[0053] Comparative Example 2. An alcohol-free, wash-free, antibacterial hand washing gel, characterized in that:

[0054] The invention is prepared from the following components by weight percentage: 1.0% trehalose, 0.1% chitosan quaternary ammonium salt, 5.0% glycerol, 10.0% propylene glycol, 0.03% polyhexamethylene biguanide hydrochloride, 0.1% didecyl dimethyl ammonium chloride, 0.1% sodium carboxymethyl cellulose, and the balance is purified water.

[0055] The preparation method of the above-mentioned alcohol-free, wash-free, antibacterial hand washing gel has the same steps as implementation case 1, and no uniform and transparent alcohol-free, wash-free, antibacterial hand washing gel is obtained by standing and defoaming.

[0056] Application Example 1

[0057] Microbial killing test

[0058] 1. Prepare suspensions of Staphylococcus aureus, Escherichia coli and Candida albicans.

[0059] 2. Disinfectant solutions should be prepared using sterile hard water unless otherwise specified.

[0060] 3. Neutralizer: 1% dodecyl, 0.25% sodium sulfite, 5% Tween, 0.5% lecithin. The neutralizer was verified by the neutralizer identification test to be effective in neutralizing the effects of the residual disinfectant gel in the three bacterial suspensions in the solution on the three tested bacteria.

[0061] 4. Organic interfering substances: 3% bovine serum albumin solution is used for suspension test; diluent: trypsin saline solution.

[0062] 5. Culture medium: Tryptone soy agar medium, Sabouraud dextrose agar medium, tryptone soy broth medium containing neutralizer.

[0063] 6. Experimental groups

[0064] Experimental group: experimental bacterial suspension + organic interfering substances + example gel; positive control: diluent instead of disinfectant, parallel test; negative control: simple culture medium

[0065] 7. Experimental results:

[0066] (1) In case 1, there was no bacterial growth in the negative control group.

[0067] Table 1 Killing effect on Escherichia coli

[0068]

[0069]

[0070] Table 2 Killing effect on Staphylococcus aureus

[0071]

[0072] Table 3 Killing effect on Candida albicans

[0073]

[0074] (2) In case 2, there was no bacterial growth in the negative control group.

[0075] Table 4 Killing effect on Escherichia coli

[0076]

[0077] Table 5 Killing effect on Staphylococcus aureus

[0078]

[0079]

[0080] Table 6 Killing effect on Candida albicans

[0081]

[0082] (3) In case 3, there was no bacterial growth in the negative control group.

[0083] Table 7 Killing effect on Escherichia coli

[0084]

[0085] Table 8 Killing effect on Staphylococcus aureus

[0086]

[0087] Table 9 Killing effect on Candida albicans

[0088]

[0089]

[0090] The above experimental results prove that the alcohol-free, wash-free, antibacterial hand washing gel prepared by the present invention has a significant antibacterial effect.

[0091] Application Example 2

[0092] Inhibition zone experiment (taking Example 1 as an example)

[0093] 1. Preparation of Staphylococcus aureus, Escherichia coli, and Candida albicans suspensions

[0094] 2. Bacterial culture medium: Tryptone soy agar medium, Sabouraud dextrose agar medium, Tryptone agar medium and Sabouraud agar medium.

[0095] 3. The antibacterial experiment was divided into an experimental group and a control group: as shown in Table 10, Group A was the experimental group, and the prescription was that of Example 1; Group BJ was the positive control group, and the concentrations of the three polycationic compounds were as shown in Table 10; and Group K was the negative control group, and the three polycationic compounds were not added. The remaining components in the prescription of the control group were the same as those in Example 1.

[0096] 4. In the experiment, 10 μl of samples from the experimental group and the control group were soaked on drug-sensitive paper with a diameter of 6 mm.

[0097] Table 10 Grouping of the control group

[0098]

[0099]

[0100] 5. Measure the diameter of the inhibition zone (mm) and calculate the average diameter of the inhibition zone of each group. The results are as follows: Figure 1 shown.

[0101] like Figure 1 As shown, group A, i.e., the alcohol-free, wash-free, antibacterial hand washing gel prepared in Example 1, has obvious antibacterial effects on Escherichia coli, Staphylococcus aureus and Candida albicans, and there is a significant difference compared with the control group BK; compared with the antibacterial effect of the control group BJ, it can be seen that the three components in the alcohol-free, wash-free, antibacterial hand washing gel prepared by the present invention have a synergistic effect.

Claims

1. An alcohol-free, wash-free, antibacterial hand washing gel, characterized in that: The invention comprises a chitosan quaternary ammonium salt, polyhexamethylene biguanide hydrochloride and didecyl dimethyl ammonium chloride composition with a mass percentage concentration not greater than 0.6% of the total amount of gel raw materials.

2. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: The amount of the polyhexamethylene biguanide hydrochloride used is less than the amount of the chitosan quaternary ammonium salt and didecyl dimethyl ammonium chloride used.

3. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: The dosage of the chitosan quaternary ammonium salt is 1-5 times that of the polyhexamethylene biguanide hydrochloride.

4. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: The dosage of the didecyl dimethyl ammonium chloride is 4-6 times the dosage of the polysaccharide quaternary ammonium salt.

5. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: It also contains a gel matrix; The amount of the gel matrix is ​​not less than 1% of the total weight of the raw materials.

6. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: The gel matrix is ​​selected from at least one of sodium carboxymethyl cellulose, methyl cellulose and hydroxypropyl methyl cellulose.

7. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: It also contains moisturizers; The amount of the moisturizing agent is not less than 1% of the total weight of the raw materials.

8. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: The moisturizing agent is selected from at least one of trehalose, hyaluronic acid and polyol.

9. The alcohol-free, wash-free, antibacterial hand washing gel according to claim 1, characterized in that: It is prepared from the following components in weight percentage:

10. The method for preparing an alcohol-free, wash-free, antibacterial hand washing gel according to claim 9, characterized in that: The following steps are involved: S1. Add part of the humectant to purified water and stir to completely dissolve it to obtain solution A; S2. Add the remaining moisturizer to solution A and mix well. Slowly add the gel matrix and chitosan quaternary ammonium salt while stirring. Stir rapidly for 10-15 minutes to make them disperse evenly. Gradually heat to 50-60°C and continue stirring to obtain a uniform and transparent gel B. S3. Add polyhexamethylene biguanide hydrochloride and didecyl dimethyl ammonium chloride to gel B, stir well to mix, and let stand to defoam to obtain a uniform, transparent, alcohol-free, wash-free, antibacterial hand washing gel.

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