Acne anti-inflammatory juice as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of cosmeceutics, and particularly relates to acne anti-inflammatory juice as well as a preparation method and application thereof. The acne anti-inflammatory juice is prepared by mixing fresh oldenlandia diffusa and fresh dandelion, squeezing juice and filtering; the weight parts of the fresh oldenlandia diffusa and the fresh dandelion are as follows: 0.5-3 parts of the fresh oldenlandia diffusa and 0.5-3 parts of the fresh dandelion. According to the invention, the fresh dandelion and the fresh oldenlandia diffusa are compounded in a specific ratio range and are matched with each other, so that a remarkable synergistic effect can be achieved, and proliferation of propionibacterium acnes can be inhibited to the greatest extent, so that acne inflammation is effectively relieved, effective components of traditional Chinese medicines can be reserved to the greatest extent, and the special pharmacodynamic effect of fresh medicines is achieved; the clinical curative effect is improved, and the problems of single form, inconvenience in clinical use and the like of a fresh medicament are solved to a certain extent.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmeceuticals, and in particular relates to an acne anti-inflammatory juice and a preparation method and application thereof. Background Art

[0002] Acne, also known as pimples, often occurs on the face and upper chest and back of adolescents. It is a chronic inflammatory skin disease that occurs in the sebaceous glands of the hair follicles of the human body, and is often manifested as acne, pustules, papules, scars, nodules and cysts. Western medicine believes that acne is mainly caused by the blockage of the ducts of the sebaceous glands of the hair follicles, which leads to excessive metabolism of sebum to produce a large amount of fatty acids, which in turn stimulates the proliferation of Propionibacterium acnes in an anaerobic environment, leading to an inflammatory reaction in the skin. The principles of Western medicine treatment are mainly to remove fat, dissolve keratin, kill bacteria, reduce inflammation and regulate hormone levels. Commonly used topical drugs include retinoic acid, benzoyl peroxide, antibiotics, azelaic acid, selenium sulfide, etc. The treatment of acne with traditional Chinese medicine includes: 1. External Chinese medicine preparations (TCM tinctures, liniments, lotions, powders, creams, fumigants, and masks); 2. Non-drug external treatments: 1. Acupuncture (fire needle therapy, filiform needle therapy, acupoint injection therapy, and bloodletting therapy); 2. Scraping therapy; 3. Auricular therapy; 4. Pricking and cupping. In order to meet the fast pace of modern society, clinical preparations of external Chinese medicine are basically made of dry products. The above-mentioned external Chinese medicine preparations are the most commonly used methods in clinical practice, but the medicinal properties of fresh Chinese medicine are different from those of dry products. Fresh Chinese medicine is mostly cold or flat, and has a stronger coldness than dry Chinese medicine of the same variety. It is suitable for all kinds of skin diseases in dermatology with acute and chronic inflammation as the main clinical manifestations. Some Chinese medicines have special effects that dry medicines do not have when used fresh, which cannot be replaced by dry medicines and have important research and clinical application value. Summary of the invention

[0003] The purpose of the present invention is to overcome the deficiencies of the above-mentioned prior art and provide an acne anti-inflammatory juice and a preparation method and application thereof.

[0004] To achieve the above objectives, the technical solutions adopted by the present invention include:

[0005] In a first aspect, the present invention provides an acne anti-inflammatory juice, which is prepared by mixing and squeezing fresh Hedyotis diffusa and fresh dandelion and filtering the juice; the weight proportion of the fresh Hedyotis diffusa is 0.5-3 parts, and the weight proportion of the fresh dandelion is 0.5-3 parts.

[0006] Dandelion contains various types of chemical components such as triterpenes, sesquiterpenes, phytosterols, flavonoids, phenolic acids, etc. Modern pharmacological research shows that it has pharmacological activities such as antibacterial, anti-endotoxin, anti-gastric damage, liver protection, hypoglycemic, anti-tumor and immunomodulatory effects; Oldenlandia diffusa mainly contains various chemical components such as cyclopentadiene ether terpenes, flavonoids, anthraquinones, polysaccharides, organic acids and trace elements, which have pharmacological effects such as anti-tumor, anti-inflammatory, antioxidant activity and immunomodulatory effects.

[0007] The acne anti-inflammatory juice of the present invention is prepared by directly mixing and squeezing fresh dandelion and fresh oldenlandia diffusa, which can not only retain the effective ingredients of traditional Chinese medicine to the greatest extent and exert the special pharmacodynamic effects of fresh medicine, but also solve the problems of single dosage form of fresh medicine and inconvenience in clinical use to a certain extent. In addition, the inventor has found through experimental exploration that after dandelion and oldenlandia diffusa are processed, the content of active substances with anti-inflammatory and antibacterial effects will be reduced, resulting in a significant reduction in the treatment of acne or anti-inflammatory effect. However, by directly using fresh dandelion and fresh oldenlandia diffusa in the above preferred ratio range, the two cooperate with each other and influence each other, and can exert a significant synergistic effect, and inhibit the proliferation of Propionibacterium acnes to the greatest extent, thereby effectively alleviating acne inflammation.

[0008] Preferably, the weight proportion of the fresh Hedyotis diffusa is 0.8-2 parts, and the weight proportion of the fresh dandelion is 1-1.5 parts.

[0009] Preferably, the weight portion of the fresh Hedyotis diffusa is 1 part, and the weight portion of the fresh dandelion is 1 part.

[0010] Experimental research has found that using equal amounts of fresh dandelions and fresh oldenlandia diffusa to prepare acne anti-inflammatory juice can fully utilize the advantages of both, with the best combined effect, significantly improving the inhibitory effect of Propionibacterium acnes, effectively alleviating or treating acne symptoms, and relieving skin inflammation. It is suitable for most people and is safe and non-toxic.

[0011] In a second aspect, the present invention provides a method for preparing an acne anti-inflammatory juice, comprising the following steps: mixing fresh Hedyotis diffusa and fresh dandelion, adding 4-6 times the amount of water, squeezing the juice, filtering, and taking the filtrate to obtain the acne anti-inflammatory juice.

[0012] When the acne anti-inflammatory juice is applied, the patient takes a suitable position according to the skin lesion site, fully exposes the skin lesion site, stacks 4-6 layers of sterilized gauze together each time, soaks them in the prepared acne anti-inflammatory juice, and then slightly twists them until no water drips, and then applies the gauze to the skin lesion, tightly, and wet compresses for 10-15 minutes each time. According to the severity of the skin lesion, wet compresses can be applied 1-3 times a day. During the wet compress process, if the gauze becomes dry, the gauze should be removed, replaced with a new gauze and re-dipped in the acne anti-inflammatory juice and twisted until no water drips to continue wet compressing. The acne anti-inflammatory juice should not be added directly to the wet compress site.

[0013] In a third aspect, the present invention provides an acne anti-inflammatory product, wherein the acne anti-inflammatory product comprises the acne anti-inflammatory juice.

[0014] Preferably, the acne anti-inflammatory product further comprises auxiliary materials.

[0015] Preferably, the product comprises a cosmetic or a medicine.

[0016] In a fourth aspect, the present invention provides use of the acne anti-inflammatory juice in the preparation of anti-acne or anti-inflammatory products.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] (1) The acne anti-inflammatory juice of the present invention is prepared by directly mixing and squeezing fresh dandelion and fresh oldenlandia diffusa, which can not only retain the effective ingredients of traditional Chinese medicine to the greatest extent and exert the special pharmacodynamic effects of fresh medicine, thereby improving the clinical efficacy, but also solve the problems of single dosage form of fresh medicine and inconvenience in clinical use to a certain extent;

[0019] (2) The present invention adopts fresh dandelion and fresh oldenlandia diffusa to compound within a specific ratio range. The two cooperate with each other and influence each other, which can play a significant synergistic effect, inhibit the proliferation of Propionibacterium acnes to the greatest extent, thereby effectively alleviating acne inflammation. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 The facial comparison pictures of patient 1 before and after the treatment with the acne anti-inflammatory juice described in Example 1;

[0021] Figure 2 The facial comparison pictures of patient 2 before and after the treatment with the acne anti-inflammatory juice described in Example 1;

[0022] Figure 3 Comparison of the face of patient 3 before and after treatment with the acne anti-inflammatory juice described in Example 1. DETAILED DESCRIPTION

[0023] To better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments. It should be understood by those skilled in the art that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0024] Unless otherwise specified, all raw materials involved in the following examples and comparative examples are commercially available general materials.

[0025] Examples 1-5

[0026] Examples 1-5 provide a formula of an acne anti-inflammatory juice (parts by weight), as shown in Table 1.

[0027] Table 1

[0028]

[0029]

[0030] The method for preparing the acne anti-inflammatory juice described in Examples 1-5 comprises the following steps:

[0031] Fresh oldenlandia diffusa and fresh dandelion are mixed and placed in a juicer, and 5 times amount of purified water is added, the juice is squeezed, filtered, and the filtrate is taken to obtain the acne anti-inflammatory juice.

[0032] Comparative Example 1

[0033] This comparative example provides an acne anti-inflammatory juice, which is different from Example 1 in that: in this comparative example, fresh Hedyotis diffusa is not added, and fresh dandelion is directly used to squeeze juice and obtain filtrate.

[0034] Comparative Example 2

[0035] This comparative example provides an acne anti-inflammatory juice, which is different from Example 1 in that: in this comparative example, fresh dandelion is not added, and fresh Hedyotis diffusa is directly used to squeeze juice and obtain filtrate.

[0036] Comparative Example 3

[0037] This comparative example provides an acne anti-inflammatory juice, which is different from Example 1 in that the weight proportions of fresh Hedyotis diffusa and fresh dandelion in this comparative example are 0.2 parts of fresh Hedyotis diffusa and 3 parts of fresh dandelion.

[0038] Comparative Example 4

[0039] This comparative example provides an acne anti-inflammatory juice, which is different from Example 1 in that the weight proportions of fresh Hedyotis diffusa and fresh dandelion in this comparative example are 3 parts of fresh Hedyotis diffusa and 0.2 parts of fresh dandelion.

[0040] Comparative Example 5

[0041] This comparative example provides an acne anti-inflammatory juice, which differs from Example 1 in that fresh purslane is substituted for fresh oldenlandia diffusa, and the juice is mixed with fresh dandelion to prepare the acne anti-inflammatory juice.

[0042] Comparative Example 6

[0043] This comparative example provides an acne anti-inflammatory juice, which is different from Example 1 in that: dry oldenlandia diffusa and dry dandelion are used to prepare the acne anti-inflammatory juice, and the specific method is: 1 part of dry oldenlandia diffusa and 1 part of dry dandelion are mixed, 5 times the amount of purified water are added, and the mixture is boiled for 1 hour, and the filtrate is filtered to obtain the acne anti-inflammatory juice.

[0044] Effect example

[0045] 1. Propionibacterium acnes inhibition test:

[0046] The acne anti-inflammatory juices prepared in Examples 1-5 and Comparative Examples 1-6 were used as samples, and the drug sensitivity test specified in the "Hygiene Standards for Disposable Hygiene Products" and pharmacopoeias of many countries was cited. The drug sensitivity effect of the test substance was evaluated by the inhibition of the number of colonies of Propionibacterium acnes by the test substance. The specific method is as follows.

[0047] Inoculate Propionibacterium acnes on a slant of Clostridium enrichment medium with agar powder and culture anaerobically at 37°C for 48-72 hours. When the colonies are fully grown, elute them with 5 mL of Clostridium enrichment medium. Collect the eluate and adjust its concentration to 10 6 CFU / mL, and the plate method was used to count the viable bacteria. Weigh 0.5 mL of each sample of Examples 1-5 and Comparative Examples 1-6 and add them to the above 0.1 mL bacterial suspension, add 9.5 mL PBS and mix, take 1 mL of each and place it in 3 parallel plates, then add Clostridium enrichment medium and culture anaerobically at 37°C for 48-72 hours, then count the colonies, repeat the above experiment 3 times, take the average, and set physiological saline as a blank control.

[0048] Antibacterial rate X = (AB) / A * 100%

[0049] Where A is the average number of colonies recovered from the blank control sample; B is the average number of colonies recovered from the test sample.

[0050] The specific results are shown in Table 2.

[0051] Table 2

[0052] Sample source Example 1 Example 2 Example 3 Example 4 Example 5 Antibacterial rate X 76.3％ 60.4％ 63.7％ 67.4％ 70.1％ Sample source Comparative Example 1 Comparative Example 2 Comparative Example 3 Comparative Example 4 Comparative Example 5 Comparative Example 6 Antibacterial rate X 35.2％ 37.1％ 51.7％ 55.4％ 57.7％ 52.3％

[0053] As can be seen from the results in Table 2, the antibacterial rate of the acne anti-inflammatory juice prepared in the embodiment of the present invention against Propionibacterium acnes reaches more than 60%, especially the antibacterial rate of the acne anti-inflammatory juice described in Example 1 reaches 76.3%, which has a good effect of inhibiting the proliferation of Propionibacterium acnes, thereby effectively alleviating acne inflammation. When only fresh dandelion or fresh Hedyotis diffusa juice was taken for experimental exploration in Comparative Examples 1-2, the antibacterial rate of Propionibacterium acnes was significantly reduced compared with Example 1. When the dosage ratio of fresh Hedyotis diffusa and fresh dandelion described in Comparative Examples 3-4 exceeded the specified range of the present invention, the antibacterial rate of the prepared acne anti-inflammatory juice also decreased. The above results show that when the fresh Hedyotis diffusa and fresh dandelion described in the present invention are compounded and squeezed to prepare acne anti-inflammatory juice at a specific ratio, the two cooperate with each other and influence each other, and can play a significant synergistic role, maximally inhibiting The anti-inflammatory effect of fresh purslane, which also has anti-inflammatory effect, was used to replace fresh oldenlandia diffusa and fresh dandelion to prepare the anti-inflammatory juice, and its antibacterial effect was also reduced compared with the embodiment, which further confirmed the synergistic effect of fresh oldenlandia diffusa and fresh dandelion, and the two were not simply combined and superimposed; and in Comparative Example 6, dried oldenlandia diffusa and dried dandelion were used to prepare the anti-inflammatory juice. Compared with the fresh raw materials, the content of active substances with anti-inflammatory and antibacterial effects was reduced after processing, resulting in a weakened antibacterial effect on Propionibacterium acnes.

[0054] 2. Safety test:

[0055] The acne anti-inflammatory juice prepared in Examples 1-5 was used as a sample to test its safety, and the specific method is as follows.

[0056] Test number: 30 people, 5 males, 25 females, aged 20-55 years old. And the volunteers meet the selection criteria for subjects in human skin patch tests in the "Technical Specifications for Safety of Cosmetics" (2015 edition).

[0057] Test method: The acne anti-inflammatory juice prepared in Examples 1-5 was subjected to patch test. Qualified patch test equipment was selected and a closed patch test method was used. About 0.020-0.025 ml of the test substance was placed in the patch tester, and applied to the curved side of the subject's forearm with external medical tape. The palm of the hand was used to gently press it to evenly apply it to the skin. The test substance patch tester was removed after 24 hours, and the skin reaction was observed 0.5, 24, and 48 hours after the removal of the test substance patch tester, and the results were recorded according to the skin reaction grading standard in the "Technical Specification for Safety of Cosmetics" (2015 edition).

[0058] The results of the human skin patch test showed that there were no positive reactions in 30 people. According to the Technical Specifications for Safety of Cosmetics (2015 edition), the test substance will not cause adverse skin reactions and the sample is safe and non-irritating.

[0059] 3. Typical cases:

[0060] (1) Huang*, female, 29 years old, had bright red erythematous papules on both cheeks at the first visit. The course of the disease lasted for 1 month. She was treated with wet compresses using the acne anti-inflammatory juice prepared in Example 1, once a day. Each time, 4-6 layers of sterilized gauze were stacked together, soaked in the prepared acne anti-inflammatory juice, and then slightly twisted until no water dripped. Then, the gauze was applied to the lesions and wet compressed for 10-15 minutes. A total of 6 treatments were performed. At the follow-up visit, the lesions were reduced. The comparison before and after treatment is shown in the figure below. Figure 1 As shown;

[0061] (2) Shen*, female, 27 years old, had facial erythema, scattered red papules and pustules at the first visit, and the course of the disease lasted for 1.5 years. She was treated with wet compresses using the acne anti-inflammatory juice prepared in Example 1, once a day. Each time, 4-6 layers of sterilized gauze were stacked together, soaked in the prepared acne anti-inflammatory juice, and then slightly twisted until no water dripped. Then, the gauze was applied to the skin lesions and wet compressed for 10-15 minutes. A total of 3 treatments were performed. At the follow-up visit, the facial erythema had faded, the red papules and pustules had become darker red than before, and some of them were dark red acne scars. The condition had improved. The comparison before and after treatment is shown in the figure below. Figure 2 As shown;

[0062] (3) Liang*, female, 31 years old, had scattered acne red papules and pustules around the mouth and forehead at the first visit, and scattered dark red acne scars. The course of the disease lasted for 2 years. She was treated with wet compresses using the acne anti-inflammatory juice prepared in Example 1, once a day. Each time, 4-6 layers of sterilized gauze were stacked together, soaked in the prepared acne anti-inflammatory juice, and then slightly twisted until no water dripped. Then, the gauze was applied to the lesions and wet compressed for 10-15 minutes. The treatment lasted for one month. At the follow-up visit, the acne around the mouth and forehead decreased, the red papules and pustules became darker red than before, most of them were dark red acne scars, and there were fewer new lesions. The comparison before and after treatment is shown in the figure below. Figure 3 shown.

[0063] Finally, it should be noted that the above embodiments are intended to illustrate the technical solution of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solution of the present invention.

Claims

1. An anti-inflammatory juice for acne, characterized in that: The acne anti-inflammatory juice is prepared by mixing fresh oldenlandia diffusa and fresh dandelion, squeezing the juice, and filtering the juice; the weight proportion of the fresh oldenlandia diffusa is 0.5-3 parts, and the weight proportion of the fresh dandelion is 0.5-3 parts.

2. The anti-inflammatory juice for acne according to claim 1, characterized in that The weight proportion of the fresh Hedyotis diffusa is 0.8-2 parts, and the weight proportion of the fresh dandelion is 1-1.5 parts.

3. The anti-inflammatory juice for acne according to claim 1, characterized in that The weight portion of the fresh Hedyotis diffusa is 1 part, and the weight portion of the fresh dandelion is 1 part.

4. A method for preparing an acne anti-inflammatory juice, characterized in that: The following steps are involved: Fresh oldenlandia diffusa and fresh dandelion are mixed, 4-6 times the amount of water is added, the juice is squeezed, filtered, and the filtrate is taken to obtain the acne anti-inflammatory juice.

5. An acne anti-inflammatory product, characterized in that: The acne anti-inflammatory product comprises the acne anti-inflammatory juice according to any one of claims 1 to 3.

6. The anti-inflammatory product for acne according to claim 5, characterized in that: The acne anti-inflammatory product also includes auxiliary materials.

7. The anti-inflammatory product for acne according to claim 5, characterized in that: The product includes a cosmetic or a medicine.

8. Use of the acne anti-inflammatory juice according to any one of claims 1 to 3 in the preparation of anti-acne or anti-inflammatory products.

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