Dynamic acoustic field modulation and particle manipulation device based on digital microfluidics

Abstract

The present invention discloses a dynamic sound field modulation and particle manipulation device based on digital microfluidics, comprising a solution tank, a digital microfluidics system, and a bulk acoustic wave resonator. The digital microfluidics system is used to dynamically modulate the sound waves generated by the bulk acoustic wave resonator. The modulated sound waves act on the solution in the solution tank to form a local acoustic flow field in the solution, thereby driving the particles in the solution and realizing dynamic manipulation of the particles. The beneficial effects of the present invention are as follows: the invented device can modulate a single sound field to turn it into a controllable dynamically changing sound field. The rapid movement of the droplets can achieve rapid changes in the sound field transmitted to the next level. The designed wedge-shaped clamping mechanism of the digital microfluidics peripherals and the chip electrodes can realize rapid replacement of the chip and stable circuit connection, while also ensuring the acoustic field coupling between the chip and the front-end sound-generating device and the back-end manipulation solution tank.

Description

Technical Field

[0001] The present invention belongs to the field of acoustofluidics, and in particular relates to a dynamic acoustic field modulation and particle manipulation device based on digital microfluidics. Background Art

[0002] Acoustic manipulation offers the advantages of contactless and remote operation, and its applications are extensive, including drug delivery, cell screening, and in vitro diagnostics. The key component of an ultrasonic particle manipulation system is an ultrasonic transducer, which converts electrical energy into mechanical vibrations, generating acoustic waves in a fluid medium. When acoustic waves interact with the fluid medium, two forces are generated: acoustic streaming force (ASF) and acoustic radiation force (ARF).

[0003] Acoustic streaming is a steady fluid flow caused by the viscous attenuation of sound waves. As sound waves propagate through a fluid, the state of each volumetric unit is characterized by fluctuations in pressure and velocity. In an ideal fluid, the time-averaged displacement of a unit cell—the net flow rate—is zero everywhere. However, in real fluids, viscous attenuation causes the net displacement of a unit cell to be non-zero during each oscillation cycle. This local effect leads to global flow, thus generating acoustic streaming.

[0004] Acoustic radiation force is generated by the transfer of wave momentum to absorbing or scattering objects in an attenuating medium. Its generation mechanism includes the dissipation of acoustic energy, reflection of the incident wave, gradient changes in the compression wave velocity, and spatial inhomogeneity of energy density in the standing wave.

[0005] Ultrasound can directly manipulate particles by exerting acoustic radiation forces, or it can indirectly drive particle motion by generating acoustic streaming through the acoustic pressure field. Particles moving in fluids are also subject to viscous drag. The ARF is proportional to the cube of the particle radius, while the ASF is proportional to the particle radius. Therefore, as the particle radius decreases, the ARF decreases faster than the ASF. At the submicron scale, the ASF begins to play a dominant role in particle motion.

[0006] With the help of acoustic radiation forces, suspended cells can be actively guided and precisely arranged at nodes or antinodes based on their compressibility and density characteristics. By changing the frequency or phase, these cell patterns can remain fixed or dynamically reconstructed. For example, Armstrong et al. used sound waves to align myoblasts within a hydrogel, thereby forming muscle tissue with bundled myotubes. These acoustically arranged high-density tissue structures better replicate the structure of natural muscle tissue and exhibit anisotropic tensile mechanical properties. Combining acoustic manipulation with microfluidic systems can also achieve the sorting of cells of different types or sizes.

[0007] Acoustic metasurfaces can be used to modulate the sound field of bulk acoustic waves. By designing and manufacturing physical microstructures, the reflection, absorption, and transmission of acoustic waves can be effectively controlled, achieving effects such as directional transmission of acoustic waves, energy convergence, and ultrasonic particle suspension. For example, focusing ultrasound through an acoustic holographic lens can be used to perform non-invasive deep-brain neuromodulation via transcranial focused ultrasound. Kai Melde et al., by combining three orthogonally arranged bulk acoustic wave resonators and three acoustic metasurfaces, were able to generate a three-dimensional holographic sound field, enabling the three-dimensional patterning of particles and cells.

[0008] The above-mentioned method of using acoustic metasurfaces to modulate the sound field, or by arranging multiple acoustic devices to generate acoustic standing waves, can already achieve patterned arrangement of particles and cells. However, this technology basically produces a static modulated sound field, or only generates a regular array pattern of lines or dots. If the sound field changes, different metasurface structures need to be redesigned and manufactured. Therefore, real-time dynamic modulation of the sound field can give acoustic operations more flexibility and has broader application prospects. Summary of the Invention

[0009] The invention discloses a dynamic sound field modulation and particle manipulation device based on digital microfluidics, which can realize dynamic manipulation of particles.

[0010] A dynamic acoustic field modulation and particle manipulation device based on digital microfluidics, including a solution tank, a digital microfluidics system, and a bulk acoustic wave resonator.

[0011] The digital microfluidic system is used to dynamically modulate the sound waves generated by the bulk acoustic wave resonator. The modulated sound waves act on the solution in the solution tank, forming a local acoustic flow field in the solution, thereby driving the particles in the solution and realizing dynamic manipulation of the particles.

[0012] Among them, the dynamic acoustic field modulation and particle manipulation device based on digital microfluidics, the digital microfluidic system includes a digital microfluidic chip, the digital microfluidic chip is composed of an upper electrode plate and a lower electrode plate, between the upper electrode plate and the lower electrode plate are droplets that can be controlled to move, the bulk acoustic wave resonator is located on the lower side of the lower electrode plate, and the solution tank is located on the upper side of the upper electrode plate.

[0013] Among them, the dynamic sound field modulation and particle manipulation device based on digital microfluidics also includes a frame, a base, a chip mounting plate and a base. The acoustic wave resonator is arranged in the frame, and the base and the base are fixedly connected by bolts; the PCB board is installed in the base, the chip mounting plate has a through hole, the bolts pass through the through hole, the chip mounting plate can slide up and down between the base and the base, the digital microfluidic chip is installed on the chip mounting plate, and the wedge-shaped gasket is inserted between the chip mounting plate and the base. The upper side of the wedge-shaped gasket is a plane, and the lower side is a wedge-shaped surface adapted to the base. The digital microfluidic chip is in contact with the PCB board and is electrically connected to the digital microfluidic control host. The base and the base are placed on the acoustic wave resonator, and the digital microfluidic chip and the acoustic wave resonator are in contact through their own weight.

[0014] Among them, the dynamic sound field modulation and particle manipulation device based on digital microfluidics has a digital microfluidic chip in close contact with the bulk acoustic wave resonator below, and coupling is performed by adding water or ultrasonic coupling agent between them to improve the transmission efficiency of bulk acoustic waves between the interfaces.

[0015] Among them, the dynamic sound field modulation and particle manipulation device based on digital microfluidics also includes a clamping mechanism, which includes a clamping block, a silicone gasket, a ball head pull rod, a straight connecting rod, a bent connecting rod and a cam. The two clamping blocks are symmetrically arranged on the left and right, one end of the clamping block is rotatably connected to the frame, and the other end is hinged to the ball head pull rod, one end of the straight connecting rod is connected to the ball head pull rod, and the other end is hinged to the cam, one end of the bent connecting rod is hinged to the cam, and the other end is connected to the ball head pull rod hinged to the clamping block on the other side, and the cam is rotatably arranged on the frame.

[0016] Wherein, in the dynamic sound field modulation and particle manipulation device based on digital microfluidics, the silicone gasket is arranged at the inner side of the clamping block.

[0017] Beneficial effects of the present invention:

[0018] (1) The device of the present invention can modulate a single sound field to transform it into a controllable and dynamically changing sound field. The rapid movement of the droplets can achieve rapid changes in the sound field transmitted to the next level.

[0019] (2) The designed wedge-shaped clamping mechanism for the digital microfluidic peripherals and chip electrodes enables rapid chip replacement and stable circuit connection, while also ensuring acoustic field coupling between the chip and the front-end sound-generating device and the back-end control solution tank;

[0020] (3) The clamping mechanism enables good integration of the BAW resonator and the digital microfluidic control system;

[0021] (4) The horizontal clamping force of the clamping mechanism allows for slight changes in the vertical dimensions of the system when it is vertically assembled;

[0022] (5) The open solution tank can reduce the limitations of controlling particles and facilitate the transfer and observation of controlled particles. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 Schematic diagram of the overall system of the present invention, Figure A is an overall assembly diagram, and Figure B is an exploded schematic diagram of the main parts;

[0024] Figure 2 It is a cross-sectional schematic diagram of the internal structure of this system;

[0025] Figure 3 Schematic diagram of the wedge-shaped clamping mechanism for the electrodes of a digital microfluidic chip, wherein Figure A shows the clamping state and Figure B shows the clamping state;

[0026] Figure 4 This is a schematic diagram of the stable clamping principle of the clamping mechanism through the cooperation between the connecting rod dead point and the cam;

[0027] Figure 5 This is a schematic diagram of the system's integrated clamping mechanism in its open state. Figures A, B, and C are the isometric view, front view, and top view, respectively.

[0028] Figure 6 This is a schematic diagram of the system's integrated clamping mechanism in its clamping state. Figures A, B, and C are the isometric view, front view, and top view, respectively.

[0029] Figure 7 This is a schematic diagram of the principle of droplet-controlled acoustic field in digital microfluidics. DETAILED DESCRIPTION

[0030] To make the technical problems solved by the present invention, the technical solutions adopted, and the technical effects achieved more clearly, the technical solutions of the present invention are further described below with reference to the accompanying drawings and through specific embodiments. It should be understood that the specific embodiments described herein are merely for the purpose of explaining the present invention and are not intended to limit the present invention. It should also be noted that, for ease of description, the drawings only show portions relevant to the present invention, not all of them.

[0031] The dynamic acoustic field modulation and particle manipulation device based on digital microfluidics includes a solution tank 1, a digital microfluidics system 2, and a bulk acoustic wave resonator 3.

[0032] The digital microfluidic system 2 is used to dynamically modulate the sound waves generated by the bulk acoustic wave resonator 3. The modulated sound waves act on the solution in the solution tank 1 to form a local acoustic flow field in the solution, thereby driving the particles in the solution and realizing dynamic manipulation of the particles.

[0033] The digital microfluidic system 2 includes a digital microfluidic chip 5, which is composed of an upper plate 22 and a lower plate 24. Between the upper plate 22 and the lower plate 24 is a droplet 21 that can be controlled to move. The bulk acoustic wave resonator 3 is located on the lower side of the lower plate 24, and the solution tank 1 is located on the upper side of the upper plate 22.

[0034] like Figure 1 、 2 As shown, the dynamic sound field modulation and particle manipulation device based on digital microfluidics also includes a frame 9, a base 7, a chip mounting plate 6 and a base 11. The acoustic wave resonator 3 is arranged in the frame 9, and the base 7 and the base 11 are fixedly connected by bolts; the PCB board 10 is installed in the base 11, the chip mounting plate 6 has a through hole, the bolts pass through the through hole, and the chip mounting plate 6 can slide up and down between the base 7 and the base 11. The digital microfluidic chip 5 is installed on the chip mounting plate 6, and the wedge-shaped gasket 8 is inserted between the chip mounting plate 6 and the base 7. The upper side of the wedge-shaped gasket 8 is a plane, and the lower side is a wedge-shaped surface adapted to the base 7. The digital microfluidic chip 5 is in contact with the PCB board 10 and is electrically connected to the digital microfluidic control host. The base 7 and the base 11 are placed on the acoustic wave resonator 3, and the digital microfluidic chip 5 and the acoustic wave resonator 3 are in contact with each other by their own weight.

[0035] Wedge-shaped clamping mechanism for electrodes in digital microfluidic chips. Figure 3 Schematic diagram of the wedge-shaped clamping mechanism for the electrodes of the digital microfluidic chip. Figure 3 As shown in A, when the wedge-shaped gasket 8 is not fully inserted, the digital microfluidic chip 5 does not contact the electrode 10-1 of the PCB board 10. At this time, the digital microfluidic chip 5 can be pushed out for chip replacement. Figure 3-Ⅰ The enlarged view of the electrodes shows that the electrode 10-1 of the PCB and the electrode 5-1 of the lower plate of the digital microfluidic chip are in a separated state. Figure 3 B) Chip mounting plate 6 is lifted horizontally, bringing PCB electrode 10-1 into close contact with electrode 5-1 on lower plate 24 of digital microfluidic chip 5, achieving a stable circuit connection. The designed step on the wedge-shaped spacer prevents excessive clamping force during insertion, ensuring a secure clamping position and facilitating removal of the wedge-shaped spacer.

[0036] The dynamic acoustic field modulation and particle manipulation device based on digital microfluidics also includes a clamping mechanism. The digital microfluidic chip 5 is in close contact with the bulk acoustic wave resonator 3 from below, and coupling is performed by adding water or ultrasonic coupling agent between them to improve the transmission efficiency of the bulk acoustic wave between the interfaces. Since the digital microfluidic chip is made of glass, in the process of clamping the bulk acoustic wave resonator and the digital microfluidic chip to make them in close contact, a clamping force is applied in the vertical direction to keep the two fixed, which can easily cause the glass chip to break. Therefore, the present invention designs a horizontal clamping mechanism to provide stable fixation after the digital microfluidic chip and the bulk acoustic wave resonator are in close contact.

[0037] like Figure 5 As shown, the clamping mechanism includes a clamping block 13, a silicone gasket 14, a ball-end tie rod 15, a straight link 16, a bent link 18, and a cam 17. Two clamping blocks 13 are symmetrically arranged. One end of the clamping block 13 is rotatably connected to the frame 9, and the other end is hinged to the ball-end tie rod 15. One end of the straight link 16 is connected to the ball-end tie rod 15, and the other end is hinged to the cam 17. One end of the bent link 18 is hinged to the cam 17, and the other end is connected to the ball-end tie rod 15, which is hinged to the other side of the clamping block 13. The cam 17 is rotatably arranged on the frame 9. The silicone gasket 14 is arranged on the inner side of the clamping block 13.

[0038] The rotation of the cam drives the straight link 16 and the curved link 18 to move, and the two links drive the clamping block 13 to swing, thus achieving the clamping process. The silicone pad 14 is used to buffer the displacement and continuously apply the clamping force.

[0039] The combination of straight link 16 and bent link 18 is adopted because the links will overlap in trajectory during movement, which makes it impossible to complete the movement process of driving the clamping block to clamp and lock. The raised part of the bent link 18 can bypass the hinge point of the straight link 16 during movement. The force of the bent link 18 is still at the hinge points at both ends, and the force form is still a two-force rod. Figure 4 The ② position can play the role of connecting rod dead point.

[0040] Figure 6 This is the state when the clamping mechanism is locked. At this time, both connecting rods have passed the dead point position and the cam 17 has been clamped. Figure A is an isometric view, Figure B is a front view, and Figure C is a top view. Figure 5 By contrast, the front view shows the process of the cam 17 driving the connecting rod to move, and the top view shows the process of the clamping block 13 swinging and clamping.

[0041] like Figure 6 As shown, the present invention adopts a crank rocker mechanism to perform the clamping action, and the cam 17 is manually rotated to drive the rocker mechanism 13 to swing in the horizontal plane, and the left and right rocker mechanisms 13 perform the clamping action.

[0042] The dead point of the crank-rocker mechanism and the cam clamping work together to achieve locking after clamping. The dead point position that can be utilized in the structure of the present invention is unstable. When the crank mechanism is subjected to disturbances such as vibration, it is easy to deviate from the dead point position, resulting in loose clamping, which is an unstable balance. Therefore, the present invention introduces a cam clamping mechanism. After the rod rotates 10°, the cam clamping is performed. Within this 10° range, the system is in a state of stable and balanced clamping. Because the clamping force at the dead point position is the largest, it is not easy to reverse back to the dead point position after rotating past the dead point position, but continued rotation will cause the cam mechanism to clamp further, and it is not easy to continue rotating. Therefore, the dead point position and cam clamping designed by the present invention work together to enable the mechanism to achieve stable clamping.

[0043] Figure 4 The diagram is the principle diagram of the horizontal clamping mechanism. The diagram only lists the connecting rod mechanism on the right to explain the motion principle. The connecting rod mechanism on the left is symmetrical about the axis of the cam. Figure 4 As shown, the clamping mechanism primarily consists of a cam and a connecting rod. One end of the connecting rod is hinged to the cam, while the other end can move horizontally. Manual rotation of the cam can cause the right end of the connecting rod to move horizontally. Counterclockwise rotation of the cam shown in the figure can move it from position ① to position ②, and then to position ③. Position ② is the dead point of the connecting rod mechanism. The connecting rod and the cam shaft are in a straight line. A horizontal force applied to the right end of the connecting rod prevents movement of the connecting rod at this position; only by rotating the cam can the rod be moved. However, position ② is an unstable dead point. Even the slightest disturbance can cause it to move out of the dead point, and the horizontal clamping force to the right from the right end of the connecting rod will cause it to move further and further away from the dead point.

[0044] To address the aforementioned issue of instability at the dead point, the present invention incorporates a cam mechanism. When the connecting rod moves to position ③, the cam clamps, preventing further counterclockwise rotation. Between positions ② and ③, the connecting rod maintains a stable locked state. This is because if a disturbance causes the left end of the connecting rod to move counterclockwise toward position ②, the clamping force increases. If the connecting rod moves clockwise toward position ③, the cam mechanism's clamping force increases. Only by manually rotating the cam counterclockwise can the connecting rod pass the dead point and move its right end to the right.

[0045] Principle of acoustic field modulation: Due to the different acoustic impedance matching between solids, liquids and gases, the propagation ability of sound waves at the solid-liquid interface and the solid-gas interface varies greatly. The formula for calculating acoustic impedance is: .

[0046] When sound waves propagate from one medium to another at an acoustic interface, they are partially reflected and partially transmitted. The acoustic reflection coefficient, R, of the interface between two materials can be calculated from their acoustic impedances.

[0047] Assuming that the acoustic impedance of medium 1 is Z1 and the acoustic impedance of medium 2 is Z2, the formula for the acoustic reflection coefficient 𝑅 is:

[0048]

[0049] This formula describes the reflection of sound waves at the interface of two media with different acoustic impedances. The reflection coefficient, R, ranges from -1 to 1. When R = 0, the acoustic impedances of the two media are equal, resulting in no reflection and all sound waves being transmitted into the second medium. When R = 1 or R = -1, there is complete reflection, with no sound waves being transmitted into the second medium. When R is between -1 and 1, some sound waves are reflected and some are transmitted.

[0050] like Figure 7 As shown, the droplets in the middle layer are in contact with the glass plates in the vertical direction, and the rest of the area around the droplets is air. In the process of sound waves transmitting upward, there are two different paths. One path passes through the air 23, and the other passes through the droplets 21. Due to the large difference in acoustic impedance between air and glass, a large amount of acoustic wave energy will be reflected between the two media of glass and air, hindering the propagation of acoustic wave energy. The difference in acoustic impedance between liquids and solids is not large, so more acoustic wave energy can be transmitted upward. Therefore, the large-scale sound field 25 can be transformed into a small-scale sound field 20 by passing through the droplets and transmitted upward, completing the modulation of the sound field.

[0051] Digital microfluidics acoustic field modulation principle. Leveraging the principle of dielectric electrowetting, digital microfluidics (DMF) systems enable highly flexible and precise droplet manipulation at the picoliter scale. The digital microfluidic chip consists of two glass plates with electrodes arranged on them. Between these plates lies a controlled droplet. By switching the electrodes on or off, and thus varying the voltage applied to the droplet's position, the droplet's movement can be conveniently manipulated.

[0052] The movement of droplets in digital microfluidic chips is very flexible and fast. The movement and distribution of droplets can dynamically control whether the sound wave energy at different positions can be transmitted to the next level, thereby changing the state of the sound field transmitted to the solution tank.

[0053] The modulated small-scale sound field is transmitted to the solution tank above. The acoustic flow generated by the bulk sound wave, the acoustic radiation force and the viscous resistance of the particles when moving in the solution work together to drive the movement of the particles in the solution tank.

[0054] The microparticles may be carbon particles, polystyrene microspheres or cells. According to an embodiment of the present invention, the particles are in an aqueous medium.

[0055] Figure 7This paper demonstrates the principle of dynamic modulation of the acoustic field using a digital microfluidic system. The digital microfluidic chip consists of an upper plate 22, a droplet 21, and a lower plate 24. The upper plate 22 is made of ITO glass, while the lower plate 24 is equipped with electrodes. Both plates are coated with a hydrophobic material. By switching the electrodes on and off on the lower plate 24, the applied voltage can change the hydrophilicity or hydrophobicity of the droplet 21, thereby controlling its movement.

[0056] The bulk acoustic wave resonator at the bottom of the digital microfluidic chip generates a large-scale uniform sound field 25. The upper and lower plates of the digital microfluidic chip are both made of glass, and the droplets are surrounded by air 23. Due to the different degrees of acoustic impedance matching, in the process of the sound field 25 at the bottom being transmitted upward, the sound waves transmitted to the air 23 interface are almost all reflected, and the sound field can only pass through the location where the droplets 21 are present. Therefore, the large-scale sound field can be modulated by the droplets 21 to become a small-scale sound field 20. As the droplets 21 move, the modulated sound field 20 will also change with the droplets. In addition, multiple droplets 21 can modulate the sound field at the same time.

[0057] A solution tank is located above the digital microfluidic chip and connected to the chip via water or ultrasonic coupling agent. The solution tank contains a solution. Microparticles are contained within the solution. These microparticles can be carbon particles, polystyrene, or cells. An acoustic field modulated by dynamic bulk acoustic wave (BAW) field modulation acts on the solution in the tank. The acoustic flow and radiation force generated by the BAW, combined with the viscous drag of the particles in the solution, drive the movement of the particles, enabling particle capture, transfer, and patterning.

[0058] The present invention is not limited to the particles mentioned in the above examples. According to the method proposed by the present invention, it is also possible to manipulate suspended particles not mentioned above or particles settled at the bottom of the solution.

[0059] In the description of the present invention, it should be noted that the terms "up", "down", "left", "right", "clockwise" and "counterclockwise" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction. Therefore, they should not be understood as limiting the present invention.

[0060] The above embodiments merely illustrate the basic principles and features of the present invention. The present invention is not limited to these embodiments. Various modifications and variations are possible without departing from the spirit and scope of the present invention. Such modifications and variations are intended to fall within the scope of the present invention. The scope of protection claimed in the present invention is defined by the appended claims and their equivalents.

Claims

1. A dynamic acoustic field modulation and particle manipulation device based on digital microfluidics, characterized by: The invention comprises a solution tank (1), a digital microfluidic system (2), and a bulk acoustic wave resonator (3); the digital microfluidic system (2) is used to dynamically modulate the acoustic wave generated by the bulk acoustic wave resonator (3); the modulated acoustic wave acts on the solution in the solution tank (1), forming a local acoustic flow field in the solution, thereby driving the particles in the solution and realizing dynamic manipulation of the particles; The digital microfluidic system (2) includes a digital microfluidic chip (5), which is composed of an upper plate (22) and a lower plate (24). A liquid droplet (21) that can be controlled to move is located between the upper plate (22) and the lower plate (24). The bulk acoustic wave resonator (3) is located below the lower plate (24), and the solution tank (1) is located above the upper plate (22). The bottom of the digital microfluidic chip (5) is in close contact with the bulk acoustic wave resonator (3), and coupling is performed by adding water or ultrasonic coupling agent between them to improve the transmission efficiency of the bulk acoustic wave between the interfaces.

2. The dynamic acoustic field modulation and particle manipulation device based on digital microfluidics according to claim 1, characterized in that: The device also includes a frame (9), a base (7), a chip mounting plate (6) and a base (11), wherein the bulk acoustic wave resonator (3) is arranged in the frame (9), and the base (7) and the base (11) are fixedly connected by bolts; the PCB board (10) is installed in the base (11), the chip mounting plate (6) has a through hole, the bolt passes through the through hole, the chip mounting plate (6) can slide up and down between the base (7) and the base (11), and the digital microfluidic chip (5) is installed on the chip. On the mounting plate (6), a wedge-shaped gasket (8) is inserted between the chip mounting plate (6) and the base (7), the upper side of the wedge-shaped gasket (8) is a plane, and the lower side is a wedge-shaped surface adapted to the base (7), the digital microfluidic chip (5) is in contact with the PCB board (10) and is electrically connected to the digital microfluidic control host, the base (7) and the base (11) are placed on the bulk acoustic wave resonator (3), and the digital microfluidic chip (5) and the bulk acoustic wave resonator (3) are in contact with each other through their own weight.

3. The dynamic acoustic field modulation and particle manipulation device based on digital microfluidics according to claim 2, characterized in that: The invention also includes a clamping mechanism, which includes a clamping block (13), a silicone gasket (14), a ball head pull rod (15), a straight connecting rod (16), a bent connecting rod (18) and a cam (17). The clamping block (13) is symmetrically arranged in two left and right directions. One end of the clamping block (13) is rotatably connected to the frame (9), and the other end is hinged to the ball head pull rod (15). One end of the straight connecting rod (16) is connected to the ball head pull rod (15), and the other end is hinged to the cam (17). One end of the bent connecting rod (18) is hinged to the cam (17), and the other end is connected to the ball head pull rod (15) hinged to the other side of the clamping block (13). The cam (17) is rotatably arranged on the frame (9).

4. The dynamic acoustic field modulation and particle manipulation device based on digital microfluidics according to claim 3, characterized in that: The silicone gasket (14) is arranged at the inner side of the clamping block (13).

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