System for monitoring, enriching and separating environmental photosynthetic microorganisms

Abstract

The invention discloses a system for monitoring, enriching and separating environmental photosynthetic microorganisms. The system comprises a pure quartz square cylinder body; four gas collection ports are formed in the top of the pure quartz square cylinder body; ten sampling ports and ten oxygen sensors are vertically arranged on each of two side walls of the pure quartz square cylinder body; and a light simulator is arranged above the pure quartz square cylinder body. According to the method, the success rate of separation and pure culture is increased, the experiment time consumption and pollution risk are remarkably reduced, and an efficient and reliable tool is provided for research of photosynthetic bacteria.

Description

Technical Field

[0001] The invention relates to a system for monitoring, enriching and separating environmental photosynthetic microorganisms. Background Art

[0002] Photosynthetic bacteria are a class of microorganisms that can convert light energy into chemical energy through photosynthesis. They are mainly divided into purple photosynthetic bacteria, green photosynthetic bacteria and cyanobacteria (also known as blue algae). These bacteria do not use water as an electron donor during photosynthesis, so they do not release oxygen, but use other compounds such as hydrogen sulfide or organic acids. Photosynthetic bacteria are widely present in nature, especially in environments with sufficient light and rich in organic matter or sulfides, such as shallow lakes and swamps. The study of photosynthetic bacteria is of great significance to the biotechnology and environmental engineering industries. First, they play a key role in the ecosystem, fixing carbon dioxide and promoting material circulation through photosynthesis, which helps to maintain ecological balance. Secondly, photosynthetic bacteria have potential industrial application value, such as in wastewater treatment, biofuel production and bioremediation. In addition, studying the photosynthetic mechanism of photosynthetic bacteria can provide reference for artificial photosynthesis and solar energy conversion technology, and promote the development of renewable energy.

[0003] However, there are many types of photosynthetic bacteria, and one of the main problems faced during isolation and pure culture is the high specificity and diversity of their environmental requirements. Different photosynthetic bacteria have different requirements for conditions such as light intensity, light quality (wavelength of light), temperature, pH, nutrients, and electron donors. For example, purple photosynthetic bacteria usually require light of a specific wavelength (such as red light or near-infrared light) to photosynthesize effectively, while green photosynthetic bacteria may have different requirements for light quality. In addition, different types of photosynthetic bacteria have significantly different tolerance ranges for temperature and pH. Some may require a warm environment, while others are suitable for growth in colder environments. These diverse requirements make it extremely complicated to simulate their natural habitats in the laboratory, resulting in a low success rate for isolation and pure culture. In addition, photosynthetic bacteria in nature often coexist with other microorganisms to form complex microbial communities. Isolating a single type of photosynthetic bacteria requires precise selective culture media and separation techniques, which further increases the difficulty.

[0004] Another significant problem is that photosynthetic bacteria grow slowly in laboratory cultures, making the isolation and pure culture process time-consuming and susceptible to external contamination. Photosynthetic bacteria are relatively inefficient at photosynthesis and their growth rates are generally slower than those of heterotrophic bacteria, making it challenging to maintain contamination-free cultures for long periods of time under laboratory conditions. Common heterotrophic bacteria in laboratory environments reproduce quickly and can easily dominate in culture media, interfering with the growth of photosynthetic bacteria. In addition, some photosynthetic bacteria may rely on symbiotic relationships with other microorganisms under natural conditions, a relationship that is difficult to fully reproduce in the laboratory, resulting in photosynthetic bacteria exhibiting lower growth rates or being completely unable to grow in pure culture.

[0005] To overcome these problems, researchers need to develop more sophisticated separation techniques and culture medium formulations, such as using specific antibacterial agents to inhibit the growth of non-target microorganisms, or trying to simulate key environmental factors in the natural habitats of photosynthetic bacteria. However, even so, the isolation and pure culture of photosynthetic bacteria is still a complex and challenging task that requires continuous technological innovation and optimization.

[0006] Thus, a system for monitoring, enrichment and isolation of environmental photosynthetic microorganisms is provided. Summary of the invention

[0007] The purpose of the present invention is to overcome the existing defects and provide a system for monitoring, enriching and separating environmental photosynthetic microorganisms, which not only improves the success rate of isolating pure culture, but also significantly reduces the experimental time and contamination risk, providing an efficient and reliable tool for the study of photosynthetic bacteria.

[0008] The technical solution to achieve the above purpose is: A system for monitoring, enriching and separating environmental photosynthetic microorganisms comprises: a pure quartz square cylinder; four gas collection ports are arranged on the top of the pure quartz square cylinder; ten sampling ports and ten oxygen sensors are vertically arranged on both side walls of the pure quartz square cylinder; and a light simulator is arranged above the pure quartz square cylinder.

[0009] Preferably, the pure quartz square cylinder is used to carry environmental samples and cultivate photosynthetic bacteria.

[0010] Preferably, the pure quartz square cylinder adopts a fully enclosed transparent pure quartz cylinder, which is used to transmit light of different wavelengths to meet the lighting requirements of different photosynthetic bacteria.

[0011] Preferably, the gas collection port is used to monitor oxygen production in the cylinder.

[0012] Preferably, the sampling port is used to extract environmental samples from different layers in the cylinder to monitor the bacterial composition.

[0013] Preferably, the oxygen sensor is used to monitor the oxygen concentration at different layers in the cylinder.

[0014] Preferably, the light simulator is used to generate light of different frequency bands.

[0015] Preferably, it also includes: A monitoring module, used for adding different light or nutrient elements into the pure quartz square cylinder and monitoring changes in physical and chemical properties; The recording and analysis module is used to record and analyze the specific physical and chemical factors required for the growth of photosynthetic bacteria, and to carry out subsequent purification and cultivation work.

[0016] Preferably, the monitoring module includes: A light adjustment unit, used for adding different lighting into the pure quartz square cylinder by adjusting the light simulator; A nutrient element regulating unit, used for adding nutrient elements into the pure quartz square cylinder; The monitoring unit is used for monitoring the changes in the physical and chemical properties of the photosynthetic bacteria in the pure quartz square cylinder under different light and different nutrient elements.

[0017] The beneficial effects of the present invention are as follows: the present invention adopts a fully enclosed transparent pure quartz cylinder body that can transmit light of different wavelengths to meet the illumination requirements of different photosynthetic bacteria, thereby solving the problem that illumination conditions are difficult to control in traditional culture methods. An environmental sample of interest is placed inside the cylinder body, and four gas collection ports are provided on the top of the cylinder body, which are closed during the experiment. When gas needs to be collected, a syringe can be used to draw gas for monitoring to determine the oxygen production of photosynthetic bacteria. Ten sampling ports and ten oxygen sensors are vertically distributed on both side walls of the cylinder body to monitor the physical and chemical properties and bacterial composition of the environmental sample, so that the oxygen production of the photosynthetic bacteria can be determined by adding While different light or nutrient elements are used to enrich photosynthetic bacteria, the changes in the physical and chemical properties of different layers of the cylinder are monitored, so as to record the physical and chemical properties of the corresponding photosynthetic bacteria and the types of photosynthetic bacteria, and then find out the specific physical and chemical factors required for the growth of the currently enriched photosynthetic bacteria, so as to carry out subsequent purification and cultivation work and ensure the stability and suitability of the culture environment; the multi-layer sampling port design of the cylinder allows researchers to sample and analyze at different layers, further improving the accuracy and reliability of separation and pure culture; the present invention significantly improves the success rate of separation and pure culture by accurately controlling and monitoring the growth environment of photosynthetic bacteria. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 It is a structural diagram of a system for monitoring, enriching and separating environmental photosynthetic microorganisms of the present invention; Figure 2 This is a structural diagram from another perspective of a system for monitoring, enriching and separating environmental photosynthetic microorganisms of the present invention; Figure 3 It is a module diagram of a system for monitoring, enriching and separating environmental photosynthetic microorganisms of the present invention; Figure 4 It is a specific module diagram of the monitoring module in the present invention.

[0019] In the figure: 1. pure quartz square cylinder; 2. gas collection port; 3. sampling port; 4. oxygen sensor; 5. light simulator; 6. monitoring module; 7. recording and analysis module; 61. light adjustment unit; 62. nutrient element adjustment unit; 63. monitoring unit. DETAILED DESCRIPTION

[0020] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0021] The present invention will be further described below in conjunction with the accompanying drawings.

[0022] like Figure 1 , 2 As shown, a system for monitoring, enriching and separating environmental photosynthetic microorganisms comprises: a pure quartz square cylinder 1; four gas collection ports 2 are arranged on the top of the pure quartz square cylinder 1; ten sampling ports 3 and ten oxygen sensors 4 are vertically arranged on both side walls of the pure quartz square cylinder 1; a light simulator 5 is arranged above the pure quartz square cylinder 1.

[0023] In the embodiment, the pure quartz square cylinder 1 is used to carry environmental samples and cultivate photosynthetic bacteria.

[0024] In the embodiment, the pure quartz square cylinder 1 adopts a fully enclosed transparent pure quartz cylinder, which is used to transmit light of different bands to meet the lighting requirements of different photosynthetic bacteria, solving the problem of difficult control of lighting conditions in traditional culture methods. The fully enclosed design can reduce external pollution and provide a more stable and controlled culture environment.

[0025] In the embodiment, the gas collection port 2 is used to monitor the oxygen production in the cylinder and the physical and chemical properties of the environmental sample to ensure the stability and suitability of the culture environment.

[0026] In the embodiment, the sampling port 3 is used to extract environmental samples at different layers in the cylinder to monitor the bacterial composition.

[0027] In the embodiment, the oxygen sensor 4 is used to monitor the oxygen concentration at different layers in the cylinder.

[0028] In the embodiment, 10 sampling ports 3 and 10 oxygen sensors 4 are vertically distributed through the two side walls of the pure quartz square cylinder 1, allowing sampling and monitoring at different levels to accurately grasp the physical and chemical properties and bacterial composition of environmental samples.

[0029] In the embodiment, the light simulator 5 is used to generate light of different frequency bands, and can meet the specific needs of different types of photosynthetic bacteria by adjusting environmental conditions such as light quality, light intensity, temperature, pH value, etc., so as to better simulate their natural habitat in the laboratory.

[0030] like Figure 3 As shown, a system for monitoring, enriching and separating environmental photosynthetic microorganisms also includes: a monitoring module 6 and a recording and analysis module 7.

[0031] The monitoring module 6 is used to add different light or nutrient elements into the pure quartz square cylinder 1 and monitor the changes in physical and chemical properties.

[0032] like Figure 4 As shown, the monitoring module 6 includes: a light adjustment unit 61 , a nutrient element adjustment unit 62 and a monitoring unit 63 .

[0033] The light adjustment unit 61 is used to add different lighting into the pure quartz square cylinder 1 by adjusting the light simulator 5 .

[0034] The nutrient element regulating unit 62 is used to add nutrient elements into the pure quartz square cylinder 1 .

[0035] The monitoring unit 63 is used to monitor the changes in the physical and chemical properties of the photosynthetic bacteria in the pure quartz square cylinder 1 under different light and different nutrient elements.

[0036] The recording and analysis module 7 is used to record and analyze the specific physical and chemical factors required for the growth of photosynthetic bacteria, and to carry out subsequent purification and cultivation work.

[0037] By loading the environmental sample into the pure quartz square cylinder 1, adding nutrient elements into the pure quartz square cylinder 1 through the nutrient element adjustment unit 62, and adjusting the environmental conditions such as light quality and light intensity through the light adjustment unit 61, the specific needs of different types of photosynthetic bacteria can be met, so as to better simulate their natural habitat in the laboratory, and the changes in physical and chemical properties can be monitored by the monitoring unit 63, so that photosynthetic bacteria can be more effectively enriched, and the specific physical and chemical factors required for the growth of photosynthetic bacteria can be recorded and analyzed through the recording and analysis module 7, and the specific physical and chemical factors required for their growth can be found out. Through sophisticated separation technology and monitoring means, the growth of non-target microorganisms can be reduced, the probability of contamination by foreign bacteria can be reduced, and the pure culture effect of photosynthetic bacteria can be ensured, thereby improving the success rate of separation and pure culture.

[0038] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions described in the above embodiments may still be modified, or some or all of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A system for monitoring, enriching and separating environmental photosynthetic microorganisms, characterized in that: include: A pure quartz square cylinder (1); four gas collection ports (2) are provided on the top of the pure quartz square cylinder (1); ten sampling ports (3) and ten oxygen sensors (4) are vertically provided on both side walls of the pure quartz square cylinder (1); and a light simulator (5) is provided above the pure quartz square cylinder (1).

2. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The pure quartz square cylinder (1) is used to carry environmental samples and cultivate photosynthetic bacteria.

3. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The pure quartz square cylinder (1) adopts a fully enclosed transparent pure quartz cylinder, which is used to transmit light of different wavelengths to meet the lighting requirements of different photosynthetic bacteria.

4. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The gas collection port (2) is used to monitor the oxygen production in the cylinder.

5. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The sampling port (3) is used to extract environmental samples at different layers in the cylinder to monitor the bacterial composition.

6. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The oxygen sensor (4) is used to monitor the oxygen concentration at different levels in the cylinder.

7. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The light simulator (5) is used to generate light in different frequency bands.

8. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: Also includes: A monitoring module, used for adding different light or nutrient elements into the pure quartz square cylinder (1) and monitoring changes in physical and chemical properties; The recording and analysis module is used to record and analyze the specific physical and chemical factors required for the growth of photosynthetic bacteria, and to carry out subsequent purification and cultivation work.

9. A system for monitoring, enriching and separating environmental photosynthetic microorganisms according to claim 1, characterized in that: The monitoring module comprises: A light adjustment unit, used for adding different lighting into the pure quartz square cylinder (1) by adjusting the light simulator (5); A nutrient element regulating unit, used for adding nutrient elements into the pure quartz square cylinder (1); The monitoring unit is used to monitor the changes in the physical and chemical properties of the photosynthetic bacteria in the pure quartz square cylinder (1) under different light and different nutrient elements.

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