China-hemp core body with bacteriostatic effect and preparation method of China-hemp core body

Abstract

The invention belongs to the technical field of bacteriostatic China-hemp materials, and particularly relates to a China-hemp core with a bacteriostatic effect and a preparation method thereof.The China-hemp core with the bacteriostatic effect is prepared from 10-20 parts of China-hemp stems, 5-10 parts of softening agent-methyl glucoside, 8-12 parts of bacteriostatic composition, 8 parts of ethyl alcohol and 6-9 parts of surfactant; glucose crystals are prepared by taking Chinese yam as a source of starch and then react with methanol to prepare a softening agent-methyl glucoside for softening a China hemp fiber material, and then a surfactant is further introduced to treat the China hemp fiber material, so that an adsorption film is formed on the surface, and the adsorption of the antibacterial composition on the surface of the China hemp fiber material is improved; meanwhile, the hemp fibers internally contain cavities and gaps, so that more active sites can be provided for adsorption of the antibacterial composition material, and the hemp core material with higher antibacterial activity is obtained.

Description

Technical Field

[0001] The invention belongs to the technical field of antibacterial hemp materials, and specifically refers to a hemp core with antibacterial effect and a preparation method thereof. Background Art

[0002] With the improvement of people's living standards and the enhancement of health awareness, consumers have higher and higher requirements for the quality and safety of sanitary products, medical supplies and other products; products with antibacterial functions can effectively reduce the risk of bacterial infection and protect the health of users, and therefore receive widespread attention and favor from consumers.

[0003] As a natural fiber material, hemp is rich in antibacterial ingredients such as cannabidiol, flavonoids, phenolic substances, etc., which can inhibit the growth of various bacteria, fungi and molds; but it also has certain shortcomings, such as the single material and limited antibacterial efficiency. The adsorption, flexibility and antibacterial properties of existing hemp core materials are often difficult to meet the demand of modern consumers for high-quality products.

[0004] In addition, there are currently no antibacterial drugs with comprehensive properties on the market that can meet people's needs. Summary of the invention

[0005] In view of the defects of the prior art, the present invention discloses a hemp core with antibacterial effect and a preparation method thereof. First, the surface of the hemp fiber is sterilized and impurities removed by ethanol, and then the hemp fiber is softened by a softener-methyl glucoside to obtain hemp fiber with good flexibility and permeability. Further, a surfactant is introduced to form an adsorption film on the surface of the hemp fiber. Then, an antibacterial composition is added. Under the action of the adsorption film, it can be well adsorbed on the surface of the hemp fiber. At the same time, the interior of the hemp fiber is rich in cavity and pore structure, which can provide more adsorption sites for the antibacterial composition, thereby obtaining a hemp core material with stronger antibacterial property.

[0006] To achieve the above object, the technical solution adopted by the present invention is: a hemp core with antibacterial effect, characterized in that it is prepared from the following raw materials in parts by weight: 10-20 parts of hemp stalks, 5-10 parts of softener-methyl glucoside, 8-12 parts of antibacterial composition, 8 parts of ethanol, and 6-9 parts of surfactant;

[0007] Furthermore, the preparation method of the softener-methyl glucoside comprises the following steps:

[0008] M1: Weigh yam and heteropoly acid in a mass ratio of 8-10:1, peel the yam, wash, dry and mash to obtain yam slurry;

[0009] M2: Add water to the yam pulp obtained in M1, stir and soak for 6 hours, wherein the mass ratio of yam pulp to water is 8-10:12, filter and remove residue to obtain a starch-rich aqueous solution, add water to the obtained starch-rich aqueous solution and filter it, repeat the water-adding and filtering operation 3 times to obtain starch, dry the starch obtained by filtration in a blast drying oven, the drying temperature is 65°C, the drying time is 24 hours, collect the powder and grind it to obtain starch;

[0010] M3: dissolving the starch obtained in step M2 with water, adding amylase and heteropolyacid, wherein the mass ratio of starch, amylase and water is 4-6:1-1.5:6, heating and stirring, the heating temperature is 50-70°C, the heating time is 10 min, adding citric acid to keep the pH range of the solution at 5-7, the concentration of citric acid is 0.5 mol / L, continuing to stir and heat, the heating temperature is 55-65°C, the heating time is 40-60 min, to obtain a glucose aqueous solution, the obtained glucose aqueous solution is dried and crystallized by a high-speed centrifugal spray dryer, the drying temperature is 90°C, the heating power is 10 kW, the atomizer speed is 20000 r / min, the drying time is 5 min, to obtain glucose crystals;

[0011] M4: Take the glucose crystals obtained in M3, stir and dissolve them in water, then add methanol and catalyst, wherein the mass ratio of glucose crystals, methanol, catalyst and water is 5-7:6-8:0.5-1:10, heat to 50-70°C, heat for 20-40 minutes, and then cool to room temperature to obtain the softener - methyl glucoside.

[0012] Furthermore, the preparation method of the antibacterial composition comprises the following steps:

[0013] N1: Weigh houttuynia cordata, dandelion, garlic, mint and ethanol in a mass ratio of 2-4:0.5-1.5:1-3:1-1.5:2; wash and dry the houttuynia cordata, dandelion, garlic and mint, and grind them with a mortar to obtain houttuynia cordata powder, dandelion powder, garlic paste and mint powder;

[0014] N2: Add water to the ethanol solution, wherein the mass ratio of ethanol to water is 2:5, to obtain an ethanol dilution solution, add houttuynia powder and dandelion powder to the ethanol dilution solution, stir and heat to obtain a mixed solution I, add garlic paste to the mixed solution I, wherein the mass ratio of garlic paste to the mixed solution I is 1-3:5, heat and stir, the heating temperature is 55-65°C, the heating time is 30-50min, continue to add mint powder, heat and stir for a second time, the heating time is 20min, then cool to room temperature, filter and remove residue to obtain an antibacterial solution;

[0015] N3: removing alcohol from the antibacterial solution obtained in step N2 by distillation, wherein the temperature for removing alcohol by distillation is 80° C. and the time for removing alcohol by distillation is 40-60 min to obtain an antibacterial aqueous solution;

[0016] N4: The antibacterial aqueous solution obtained in step N3 is centrifuged at a centrifugal speed of 10000 r / min for 10 min to obtain an antibacterial composition precursor, and the antibacterial composition precursor is dried in a blast drying oven at a drying temperature of 55° C. for 24 h. The powder is collected and ground to obtain an antibacterial composition.

[0017] The present invention also provides a method for preparing a hemp core with antibacterial effect, comprising the following steps:

[0018] Step I, weighing 10-20 parts of hemp stalks, 5-10 parts of softener-methyl glucoside, 8-12 parts of antibacterial composition, 8 parts of ethanol, and 6-9 parts of surfactant;

[0019] Step II, peeling off the bast from the hemp stalks weighed in step I and crushing them to obtain hemp fiber powder;

[0020] Step III, adding the hemp fiber powder obtained in step II to a mixed solution of ethanol and water, wherein the mass ratio of hemp fiber powder, ethanol and water is 10-20:8:15, stirring at room temperature for 30 minutes, and then filtering to obtain sterilized hemp fiber powder;

[0021] Step VI, adding softener-methyl glucoside into water and stirring to dissolve, wherein the mass ratio of softener-methyl glucoside to water is 6-8:10, to obtain a softener-methyl glucoside aqueous solution, adding the sterilized hemp fiber powder obtained in step III into the softener-methyl glucoside aqueous solution, wherein the mass ratio of sterilized hemp fiber powder to softener-methyl glucoside aqueous solution is 10-15:12, heating and stirring, the heating temperature is 60-80°C, the heating time is 30-50min, and filtering to obtain softened hemp fiber powder;

[0022] Step V, adding the surfactant weighed in step I to a mixed solution of ethanol and water, wherein the mass ratio of the surfactant, ethanol and water is 6-9:6:10, heating and stirring, the heating temperature is 55-65°C, and the heating time is 20-40min, to obtain a mixed solution II, adding the softened hemp fiber powder obtained in step VI to the mixed solution II, wherein the mass ratio of the softened hemp fiber powder to the mixed solution II is 6-10:8, ultrasonic treatment, the ultrasonic power is 0.5kw, the ultrasonic time is 25-35min, and then continuing to stir and heat, the heating temperature is 60°C, and the heating time is 30min, to obtain an adsorbent fiber material mixed solution;

[0023] Step IV, adding the antibacterial composition to the mixed liquid of the adsorbent fiber material obtained in step V, wherein the mass ratio of the antibacterial composition to the adsorbent fiber material is 8-12:15, ultrasonic treatment, the ultrasonic power is 0.5kw, the ultrasonic time is 10-20min, and then heating and stirring are continued, the heating temperature is 60°C, the heating time is 30min, cooling to room temperature and standing for 2h, filtering and removing the mixed liquid to obtain the hemp core material, using a blast drying oven for drying treatment, the drying temperature is 65°C, the drying time is 24h, and the material is collected to obtain the hemp core with antibacterial effect.

[0024] The beneficial effects achieved by the present invention are as follows:

[0025] The hemp core with antibacterial effect prepared by the present invention is the first to prepare a hemp core with high antibacterial properties through a series of reactions. During the preparation process, the surface of the hemp fiber is firstly sterilized and decontaminated by ethanol, and then the hemp fiber is further softened by the prepared softener-methyl glucoside to improve the overall flexibility and permeability of the material. In order to improve the adsorption of the antibacterial composition on the surface of the hemp fiber, a surfactant is introduced to treat and soften the hemp fiber powder to form an adsorption film on its surface to improve the adsorption of the antibacterial composition. In addition, the hemp fiber has a structure containing cavities and gaps, which can provide more adsorption sites for the adsorption of the antibacterial composition material, thereby improving the overall antibacterial effect of the prepared hemp core.

[0026] The hemp core with antibacterial effect prepared by the present invention uses yam as the source of starch for the first time, adjusts the pH by citric acid, and improves the yield of starch hydrolysis converted into glucose under the catalytic action of amylase and heteropolyacid. The obtained glucose is glycosylated with methanol under the action of a catalyst to prepare a softener - methyl glucoside, which can soften hemp fiber and make the material more flexible.

[0027] The hemp core with antibacterial effect prepared by the present invention improves the overall antibacterial effect of the hemp core from the level of loading materials for the first time. At the same time, the selected source materials are green and non-toxic and have no side effects on the human body. The obtained hemp core with antibacterial effect has a good antibacterial effect on Staphylococcus aureus, Candida albicans and Escherichia coli. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] Figure 1 A diagram showing a preparation method according to an embodiment of the present invention;

[0029] Figure 2 This is a diagram of the antibacterial effect of the comparative example of the present invention;

[0030] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0031] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0032] Unless otherwise defined, all professional and scientific terms used herein have the same meanings as those familiar to those skilled in the art. In addition, any methods and materials similar or equivalent to those described herein may be applied to the present invention. The preferred implementation methods and materials described herein are for demonstration purposes only and are not intended to limit the content of this application.

[0033] The preparation methods in the following examples are referred to Figure 1 , unless otherwise specified, are conventional methods; the materials used in the following embodiments, unless otherwise specified, are all new materials purchased from the market, and the preferred amylase and catalyst of the present invention are saccharifying enzyme and β-glucosidase.

[0034] Example 1: A hemp core with antibacterial effect, characterized in that it is prepared from the following raw materials in parts by weight: 10 parts of hemp stalks, 5 parts of softener-methyl glucoside, 8 parts of antibacterial composition, 8 parts of ethanol, and 6 parts of surfactant;

[0035] Furthermore, the preparation method of the softener-methyl glucoside comprises the following steps:

[0036] M1: Weigh yam and heteropoly acid in a mass ratio of 8:1, peel the yam, wash, dry and mash to obtain yam slurry;

[0037] M2: Add water to the yam pulp obtained in M1, stir and soak for 6 hours, wherein the mass ratio of yam pulp to water is 8:12, filter and remove residue to obtain a starch-rich aqueous solution, add water and filter the starch-rich aqueous solution, repeat the water-adding and filtering operation 3 times to obtain starch, dry the starch obtained by filtration in a forced air drying oven at 65° C. for 24 hours, collect the powder and grind it to obtain starch;

[0038] M3: Dissolve the starch obtained in step M2 in water, then add saccharifying enzyme and heteropoly acid thereto, wherein the mass ratio of starch, saccharifying enzyme and water is 4:1:6, heat and stir, the heating temperature is 50°C, the heating time is 10 min, add citric acid with a concentration of 0.5 mol / L to keep the solution pH range at 5, continue stirring and heating to 55°C, the heating time is 40 min, obtain a glucose aqueous solution, and use a high-speed centrifugal spray dryer to dry and crystallize the obtained glucose aqueous solution, wherein the heating power is 10 kW, the atomizer speed is 20000 r / min, the drying temperature is 90°C, the drying time is 5 min, and then obtain glucose crystals;

[0039] M4: Take the glucose crystals obtained in M3, stir and dissolve them in water, then add methanol and β-glucosidase, wherein the mass ratio of glucose crystals, methanol, β-glucosidase and water is 5:6:0.5:10, heat to 50°C for 20 minutes, then cool to room temperature to obtain the softener - methyl glucoside.

[0040] Furthermore, the preparation method of the antibacterial composition comprises the following steps:

[0041] N1: Weigh houttuynia cordata, dandelion, garlic, mint and ethanol in a mass ratio of 2:0.5:1:1:2; wash and dry the houttuynia cordata, dandelion, garlic and mint, and grind them with a mortar to obtain houttuynia cordata powder, dandelion powder, garlic paste and mint powder;

[0042] N2: Add water to the ethanol solution, wherein the mass ratio of ethanol to water is 2:5, to obtain an ethanol dilution solution, add houttuynia powder and dandelion powder to the ethanol dilution solution, stir and heat to obtain a mixed solution I, further add garlic paste to the mixed solution I, wherein the mass ratio of garlic paste to the mixed solution I is 1:5, stir and heat, heat to 55°C, and the heating time is 30 minutes, continue to add mint powder, heat and stir for a second time, and the heating time is 20 minutes, then cool to room temperature, filter and remove residue to obtain an antibacterial solution;

[0043] N3: The antibacterial solution obtained in step N2 is distilled to remove alcohol at a temperature of 80° C. The distillation time is 40 minutes to obtain an antibacterial aqueous solution;

[0044] N4: The antibacterial aqueous solution obtained in step N3 is centrifuged at a centrifugal speed of 10000r / min for 10min to obtain an antibacterial composition precursor, and the antibacterial composition precursor is dried in a blast drying oven at a temperature of 55°C for 24h, and the powder is collected and ground to obtain an antibacterial composition.

[0045] The present invention also provides a method for preparing a hemp core with antibacterial effect, comprising the following steps:

[0046] Step I, weighing 10 parts of hemp stalks, 5 parts of softener-methyl glucoside, 8 parts of antibacterial composition, 8 parts of ethanol, and 6 parts of surfactant;

[0047] Step II, peeling off the bast from the hemp stalks weighed in step I and crushing them to obtain hemp fiber powder;

[0048] Step III, adding the hemp fiber powder obtained in step II to a mixed solution of ethanol and water, wherein the mass ratio of hemp fiber powder, ethanol and water is 10:8:15, stirring at room temperature for 30 minutes, and then filtering to obtain sterilized hemp fiber powder;

[0049] Step VI, adding softener-methyl glucoside into water and stirring to dissolve, wherein the mass ratio of softener-methyl glucoside to water is 6:10, to obtain a softener-methyl glucoside aqueous solution, adding the sterilized hemp fiber powder obtained in step III into the softener-methyl glucoside aqueous solution, wherein the mass ratio of sterilized hemp fiber powder to softener-methyl glucoside aqueous solution is 10:12, heating and stirring, heating to 60° C., heating time is 30 min, filtering to obtain softened hemp fiber powder;

[0050] Step V, adding the surfactant weighed in step I to a mixed solution of ethanol and water, wherein the mass ratio of the surfactant, ethanol and water is 6:6:10, heating and stirring, heating to 55°C, heating time is 20 minutes, to obtain a mixed solution II, adding the softened hemp fiber powder obtained in step VI to the mixed solution II, wherein the mass ratio of the softened hemp fiber powder to the mixed solution II is 6:8, performing ultrasonic treatment under the condition of an ultrasonic power of 0.5 kW, ultrasonic time is 25 minutes, and then continuing to stir and heat, heating to 60°C, heating time is 30 minutes, to obtain an adsorbent fiber material mixed solution;

[0051] Step IV, adding the antibacterial composition to the mixed liquid of the adsorbent fiber material obtained in step V, wherein the mass ratio of the antibacterial composition to the adsorbent fiber material is 8:15, performing ultrasonic treatment under the condition of an ultrasonic power of 0.5 kW and an ultrasonic time of 10 min, then continuing heating and stirring, heating to 60°C for 30 min, cooling to room temperature and standing for 2 h, filtering and removing the mixed liquid to obtain the hemp core material, using a blast drying oven for drying, the drying temperature is 65°C, the drying time is 24 h, and the material is collected to obtain a hemp core with antibacterial effect.

[0052] Example 2: A hemp core with antibacterial effect, characterized in that it is prepared from the following raw materials in parts by weight: 15 parts of hemp stalks, 8 parts of softener-methyl glucoside, 10 parts of antibacterial composition, 8 parts of ethanol, and 7.5 parts of surfactant;

[0053] Furthermore, the preparation method of the softener-methyl glucoside comprises the following steps:

[0054] M1: Weigh yam and heteropoly acid in a mass ratio of 9:1, peel the yam, wash, dry and mash to obtain yam slurry;

[0055] M2: Add water to the yam pulp obtained in M1, stir and soak for 6 hours, wherein the mass ratio of yam pulp to water is 9:12, filter and remove residue to obtain a starch-rich aqueous solution, add water and filter the starch-rich aqueous solution, repeat the water-adding and filtering operation 3 times to obtain starch, dry the starch obtained by filtration in a forced air drying oven at 65° C. for 24 hours, collect the powder and grind it to obtain starch;

[0056] M3: Dissolve the starch obtained in step M2 in water, then add saccharifying enzyme and heteropoly acid thereto, wherein the mass ratio of starch, saccharifying enzyme and water is 5:1.3:6, heat and stir, the heating temperature is 60°C, the heating time is 10 min, add citric acid with a concentration of 0.5 mol / L to keep the solution pH range at 6, continue stirring and heating to 60°C, the heating time is 50 min, to obtain a glucose aqueous solution, and use a high-speed centrifugal spray dryer to dry and crystallize the obtained glucose aqueous solution, wherein the heating power is 10 kW, the atomizer speed is 20000 r / min, the drying temperature is 90°C, the drying time is 5 min, and then glucose crystals are obtained;

[0057] M4: Take the glucose crystals obtained in M3, stir and dissolve them in water, then add methanol and β-glucosidase, wherein the mass ratio of glucose crystals, methanol, β-glucosidase and water is 6:7:0.8:10, heat to 60°C for 30 minutes, then cool to room temperature to obtain the softener - methyl glucoside.

[0058] Furthermore, the preparation method of the antibacterial composition comprises the following steps:

[0059] N1: Weigh houttuynia cordata, dandelion, garlic, mint and ethanol in a mass ratio of 3:1:2:1.3:2; wash and dry the houttuynia cordata, dandelion, garlic and mint, and grind them with a mortar to obtain houttuynia cordata powder, dandelion powder, garlic paste and mint powder;

[0060] N2: Add water to the ethanol solution, wherein the mass ratio of ethanol to water is 2:5, to obtain an ethanol dilution solution, add houttuynia powder and dandelion powder to the ethanol dilution solution, stir and heat to obtain a mixed solution I, further add garlic paste to the mixed solution I, wherein the mass ratio of garlic paste to the mixed solution I is 2:5, stir and heat, heat to 60°C, the heating time is 40 minutes, continue to add mint powder, heat and stir for a second time, the heating time is 20 minutes, then cool to room temperature, filter and remove residue to obtain an antibacterial solution;

[0061] N3: The antibacterial solution obtained in step N2 is distilled to remove alcohol at a temperature of 80° C. The distillation time is 50 min to obtain an antibacterial aqueous solution;

[0062] N4: The antibacterial aqueous solution obtained in step N3 is centrifuged at a centrifugal speed of 10000r / min for 10min to obtain an antibacterial composition precursor, and the antibacterial composition precursor is dried in a blast drying oven at a temperature of 55°C for 24h, and the powder is collected and ground to obtain an antibacterial composition.

[0063] The present invention also provides a method for preparing a hemp core with antibacterial effect, comprising the following steps:

[0064] Step I, weighing 15 parts of hemp stalks, 8 parts of softener-methyl glucoside, 10 parts of antibacterial composition, 8 parts of ethanol, and 7.5 parts of surfactant;

[0065] Step II, peeling off the bast from the hemp stalks weighed in step I and crushing them to obtain hemp fiber powder;

[0066] Step III, adding the hemp fiber powder obtained in step II to a mixed solution of ethanol and water, wherein the mass ratio of hemp fiber powder, ethanol and water is 15:8:15, stirring at room temperature for 30 minutes, and then filtering to obtain sterilized hemp fiber powder;

[0067] Step VI, adding softener-methyl glucoside into water and stirring to dissolve, wherein the mass ratio of softener-methyl glucoside to water is 7:10, to obtain a softener-methyl glucoside aqueous solution, adding the sterilized hemp fiber powder obtained in step III into the softener-methyl glucoside aqueous solution, wherein the mass ratio of sterilized hemp fiber powder to softener-methyl glucoside aqueous solution is 13:12, heating and stirring, heating to 70° C., heating time is 40 min, and filtering to obtain softened hemp fiber powder;

[0068] Step V, adding the surfactant weighed in step I to a mixed solution of ethanol and water, wherein the mass ratio of the surfactant, ethanol and water is 7.5:6:10, heating and stirring, heating to 60°C, heating time is 30 minutes, to obtain a mixed solution II, adding the softened hemp fiber powder obtained in step VI to the mixed solution II, wherein the mass ratio of the softened hemp fiber powder to the mixed solution II is 8:8, performing ultrasonic treatment under the condition of an ultrasonic power of 0.5 kW, ultrasonic time is 30 minutes, and then continuing to stir and heat, heating to 60°C, heating time is 30 minutes, to obtain an adsorbent fiber material mixed solution;

[0069] Step IV, adding the antibacterial composition to the mixed solution of the adsorbent fiber material obtained in step V, wherein the mass ratio of the antibacterial composition to the adsorbent fiber material is 10:15, performing ultrasonic treatment under the condition of an ultrasonic power of 0.5 kW and an ultrasonic time of 15 min, then continuing heating and stirring, heating to 60°C for 30 min, cooling to room temperature and standing for 2 h, filtering and removing the mixed solution to obtain the hemp core material, using an air drying oven for drying, the drying temperature is 65°C, the drying time is 24 h, and the material is collected to obtain a hemp core with antibacterial effect.

[0070] Example 3: A hemp core with antibacterial effect, characterized in that it is prepared from the following raw materials in parts by weight: 20 parts of hemp stalks, 10 parts of softener-methyl glucoside, 12 parts of antibacterial composition, 8 parts of ethanol, and 9 parts of surfactant;

[0071] Furthermore, the preparation method of the softener-methyl glucoside comprises the following steps:

[0072] M1: Weigh yam and heteropoly acid in a mass ratio of 10:1, peel the yam, wash, dry and mash to obtain yam slurry;

[0073] M2: Add water to the yam pulp obtained in M1, stir and soak for 6 hours, wherein the mass ratio of yam pulp to water is 10:12, filter and remove residue to obtain a starch-rich aqueous solution, add water and filter the starch-rich aqueous solution, repeat the water-adding and filtering operation 3 times to obtain starch, dry the starch obtained by filtration in a forced air drying oven at 65° C. for 24 hours, collect the powder and grind it to obtain starch;

[0074] M3: Dissolve the starch obtained in step M2 in water, then add saccharifying enzyme and heteropoly acid thereto, wherein the mass ratio of starch, saccharifying enzyme and water is 6:1.5:6, heat and stir, the heating temperature is 70°C, the heating time is 10 min, add citric acid with a concentration of 0.5 mol / L to keep the solution pH range at 7, continue stirring and heating to 65°C, the heating time is 60 min, to obtain a glucose aqueous solution, and use a high-speed centrifugal spray dryer to dry and crystallize the obtained glucose aqueous solution, wherein the heating power is 10 kW, the atomizer speed is 20000 r / min, the drying temperature is 90°C, the drying time is 5 min, and then glucose crystals are obtained;

[0075] M4: Take the glucose crystals obtained in M3, stir and dissolve them in water, then add methanol and β-glucosidase, wherein the mass ratio of glucose crystals, methanol, β-glucosidase and water is 7:8:1:10, heat to 70°C for 40 minutes, then cool to room temperature to obtain the softener - methyl glucoside.

[0076] Furthermore, the preparation method of the antibacterial composition comprises the following steps:

[0077] N1: Weigh houttuynia cordata, dandelion, garlic, mint and ethanol in a mass ratio of 4:1.5:3:1.5:2; wash and dry the houttuynia cordata, dandelion, garlic and mint, and grind them with a mortar to obtain houttuynia cordata powder, dandelion powder, garlic paste and mint powder;

[0078] N2: Add water to the ethanol solution, wherein the mass ratio of ethanol to water is 2:5, to obtain an ethanol dilution solution, add houttuynia powder and dandelion powder to the ethanol dilution solution, stir and heat to obtain a mixed solution I, further add garlic paste to the mixed solution I, wherein the mass ratio of garlic paste to the mixed solution I is 3:5, stir and heat, heat to 65°C, the heating time is 50 min, continue to add mint powder, heat and stir for a second time, the heating time is 20 min, then cool to room temperature, filter and remove residue to obtain an antibacterial solution;

[0079] N3: The antibacterial solution obtained in step N2 is distilled to remove alcohol at a temperature of 80° C. The distillation time is 60 minutes to obtain an antibacterial aqueous solution;

[0080] N4: The antibacterial aqueous solution obtained in step N3 is centrifuged at a centrifugal speed of 10000r / min for 10min to obtain an antibacterial composition precursor, and the antibacterial composition precursor is dried in a blast drying oven at a temperature of 55°C for 24h, and the powder is collected and ground to obtain an antibacterial composition.

[0081] The present invention also provides a method for preparing a hemp core with antibacterial effect, comprising the following steps:

[0082] Step I, weighing 20 parts of hemp stalks, 10 parts of softener-methyl glucoside, 12 parts of antibacterial composition, 8 parts of ethanol, and 9 parts of surfactant;

[0083] Step II, peeling off the bast from the hemp stalks weighed in step I and crushing them to obtain hemp fiber powder;

[0084] Step III, adding the hemp fiber powder obtained in step II to a mixed solution of ethanol and water, wherein the mass ratio of hemp fiber powder, ethanol and water is 20:8:15, stirring at room temperature for 30 minutes, and then filtering to obtain sterilized hemp fiber powder;

[0085] Step VI, adding softener-methyl glucoside into water and stirring to dissolve, wherein the mass ratio of softener-methyl glucoside to water is 8:10, to obtain a softener-methyl glucoside aqueous solution, adding the sterilized hemp fiber powder obtained in step III into the softener-methyl glucoside aqueous solution, wherein the mass ratio of sterilized hemp fiber powder to softener-methyl glucoside aqueous solution is 15:12, heating and stirring, heating to 80° C., heating time is 50 min, and filtering to obtain softened hemp fiber powder;

[0086] Step V, adding the surfactant weighed in step I to a mixed solution of ethanol and water, wherein the mass ratio of the surfactant, ethanol and water is 9:6:10, heating and stirring, heating to 65°C, heating time is 40 minutes, to obtain a mixed solution II, adding the softened hemp fiber powder obtained in step VI to the mixed solution II, wherein the mass ratio of the softened hemp fiber powder to the mixed solution II is 10:8, performing ultrasonic treatment under the condition of an ultrasonic power of 0.5 kW, ultrasonic time is 35 minutes, and then continuing to stir and heat, heating to 60°C, heating time is 30 minutes, to obtain an adsorbent fiber material mixed solution;

[0087] Step IV, adding the antibacterial composition to the mixed solution of the adsorbent fiber material obtained in step V, wherein the mass ratio of the antibacterial composition to the adsorbent fiber material is 12:15, performing ultrasonic treatment under the condition of an ultrasonic power of 0.5 kW and an ultrasonic time of 20 min, then continuing heating and stirring, heating to 60°C for 30 min, cooling to room temperature and standing for 2 h, filtering and removing the mixed solution to obtain the hemp core material, using a blast drying oven for drying, the drying temperature is 65°C, the drying time is 24 h, and the material is collected to obtain a hemp core with antibacterial effect.

[0088] The difference between Comparative Example 1 and Example 2 is that no softener-methyl glucoside is added, and the rest is the same as Example 2;

[0089] The difference between Comparative Example 2 and Example 2 is that no antibacterial composition is added, and the rest is the same as Example 2;

[0090] The difference between Comparative Example 3 and Example 2 is that no surfactant is added, and the rest is the same as Example 2;

[0091] The difference between Comparative Example 4 and Example 2 is that no softener-methyl glucoside and antibacterial composition are added, and the rest is the same as Example 2;

[0092] The difference between Comparative Example 5 and Example 2 is that no softener-methyl glucoside and surfactant are added, and the rest is the same as Example 2;

[0093] The difference between Comparative Example 6 and Example 2 is that no antibacterial composition and surfactant are added, and the rest is the same as Example 2;

[0094] The difference between Comparative Example 7 and Example 2 is that no softener-methyl glucoside, antibacterial composition and surfactant are added, and the rest is the same as Example 2;

[0095] The following tests were conducted on the antibacterial effect of the prepared hemp core with antibacterial effect: the antibacterial properties of three bacterial species, Staphylococcus aureus, Candida albicans and Escherichia coli, were studied. Sterile physiological saline was used to prepare the above microorganisms at an initial bacterial concentration of 100 CFU / ml to obtain a uniform microbial suspension for subsequent antibacterial effect testing.

[0096] Weigh 0.3g of the prepared hemp core with antibacterial effect, and use a tablet press to press it into a hemp core sheet of 10mm*10mm*1mm; set a blank group, that is, do not take any antibacterial measures, the test conditions are a constant temperature of 37°C and a humidity of 30%, put the prepared hemp core sheets obtained in Example 1, Example 2 and Example 3 into a culture box containing a breathable membrane, sterilize the environment in the culture box, and then add 5ml of nutrient solution required for bacterial growth to the hemp core sheet as a culture basis for the bacterial strain, and then further add a microbial suspension, shake it thoroughly before adding, and then use a sterile pipette to absorb 3ml of the microbial suspension, and evenly drop it on the surface of the hemp core sheet, culture for 24h, and then detect the concentration of the bacterial strain.

[0097] After 24 hours of cultivation, use tweezers to fix and absorb 1 ml of the microbial suspension after bacterial culture on the surface of the hemp core sheet. The unit of bacterial calculation is CFU / ml.

[0098] Table 1 shows the antibacterial effect test results of the hemp core with antibacterial effect prepared in Example:

[0099]

[0100] It can be seen from the results in Table 1 above that under an environment suitable for bacterial growth, after 24 hours of bacterial culture, the number of bacterial species in Example 1, Example 2 and Example 3 did not increase significantly compared with the initial amount, and the number of bacterial species in Example 2 was lower than that in Example 1 and Example 3, indicating that the hemp core prepared in Example 2 has the best antibacterial effect. At the same time, with reference to the blank group, it can be seen that without any antibacterial measures, the number of bacterial species increased sharply.

[0101] The antibacterial effect of the prepared comparative example was tested, and the test method and conditions were the same as those in the embodiment. The test results are as follows: Figure 2 As shown, through Figure 2 It can be seen from the results that under an environment suitable for bacterial growth, after 24 hours of bacterial culture, the number of bacteria in the control group changed significantly compared with the initial value of 100 CFU / ml under different preparation conditions. The number of bacteria without adding a surfactant was higher than that with adding a surfactant, indicating that during the preparation process, the adsorption film formed has a strong adsorption capacity for the antibacterial composition, thereby improving the overall antibacterial property.

[0102] In order to further test the clinical trial effect of the prepared hemp core with antibacterial effect, 6 patients with skin injuries on their hands were selected to carry out antibacterial test using the hemp core of the prepared embodiment, with 2 people in a group. The skin injury area was first disinfected with saline, and then the prepared hemp core sheet was applied on it, and then bandaged with gauze, which was changed once a day. Within 3 days of use, no symptoms such as infection occurred. At the same time, the material was comfortable to use without discomfort.

[0103] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0104] The present invention and its implementation methods are described above, which is not restrictive. The drawings are only one of the implementation methods of the present invention, and the actual application is not limited thereto. In short, if ordinary technicians in the field are inspired by it and design methods and embodiments similar to the technical solution without creativity without departing from the purpose of the invention, they should all fall within the protection scope of the present invention.

Claims

1. A hemp core with antibacterial effect, characterized in that: The invention is prepared from the following raw materials in parts by weight: 10-20 parts of hemp stalks, 5-10 parts of softener-methyl glucoside, 8-12 parts of antibacterial composition, 8 parts of ethanol, and 6-9 parts of surfactant; The raw materials for preparing the softener-methyl glucoside include yam, amylase, heteropoly acid, methanol and citric acid; The raw materials for preparing the antibacterial composition include houttuynia cordata, dandelion, garlic, mint and ethanol, and the mass ratio of houttuynia cordata, dandelion, garlic, mint and ethanol is 2-4: 0.5-1.5:1-3:1-1.5:2。 2. The hemp core with antibacterial effect according to claim 1, characterized in that: The preparation method of the softener-methyl glucoside comprises the following steps: M1: Weigh yam and heteropoly acid, peel the yam, wash, dry and mash to obtain yam slurry; M2: adding water to the yam slurry obtained in M1, stirring and soaking the slurry, filtering and removing residues to obtain a starch-rich aqueous solution, adding water and filtering the obtained starch-rich aqueous solution, repeating the water-adding and filtering operation to obtain starch, drying the starch obtained by filtration, collecting the powder and grinding it to obtain starch; M3: dissolving the starch obtained in step M2 with water, adding amylase and heteropoly acid, heating and stirring, adding citric acid to maintain the solution pH range, continuing stirring and heating to obtain a glucose aqueous solution, and drying and crystallizing the obtained glucose aqueous solution to obtain glucose crystals; M4: Take the glucose crystals obtained in M3 and stir to dissolve in water, then add methanol and a catalyst, stir and heat, and then cool to room temperature to obtain a softener - methyl glucoside.

3. The hemp core with antibacterial effect according to claim 2, characterized in that: In step M1, the mass ratio of yam and heteropoly acid is 8-10:1; in step M2, the mass ratio of yam slurry and water is 8-10:12, and a blast drying oven is used for drying, the drying temperature is 65°C, and the drying time is 24h; in step M3, the mass ratio of starch, amylase and water is 4-6:1-1.5:6, the heating temperature is 50-70°C, the heating time is 10min, the citric acid concentration is 0.5mol / L, the pH range is 5-7, the heating temperature is 55-65°C, the time is 40-60min, a high-speed centrifugal spray dryer is used for drying and crystallization, the drying temperature is 90°C, the heating power is 10kw, the atomizer speed is 20000r / min, and the drying time is 5min. The mass ratio of glucose crystals, methanol, catalyst and water in M4 is 5-7:6-8:0.5-1:10, the heating temperature is 50-70°C, and the heating time is 20-40min.

4. The hemp core with antibacterial effect according to claim 1, characterized in that: The method for preparing the antibacterial composition comprises the following steps: N1: Weigh houttuynia cordata, dandelion, garlic, mint and ethanol; wash and dry the houttuynia cordata, dandelion, garlic and mint, and grind them with a mortar to obtain houttuynia cordata powder, dandelion powder, garlic paste and mint powder; N2: adding water to the ethanol solution to obtain an ethanol diluted solution, adding houttuynia powder and dandelion powder to the ethanol diluted solution, stirring and heating to obtain a mixed solution I, adding garlic paste to the mixed solution I, heating and stirring, continuing to add mint powder, heating and stirring for a second time, then cooling to room temperature, filtering and removing residues to obtain an antibacterial solution; N3: removing alcohol from the antibacterial solution obtained in step N2 by distillation to obtain an antibacterial aqueous solution; N4: centrifuge the antibacterial aqueous solution obtained in step N3 to obtain an antibacterial composition precursor, dry the antibacterial composition precursor, collect the powder and grind it to obtain an antibacterial composition.

5. The hemp core with antibacterial effect according to claim 4, characterized in that: The mass ratio of ethanol and water in step N2 is 2:5, the mass ratio of mashed garlic and mixed solution I is 1-3:5, the heating temperature is 55-65°C, the heating time is 30-50min, and the secondary heating time is 20min; the temperature for distilling and removing alcohol in step N3 is 80°C, and the distillation and removing alcohol time is 40-60min; the centrifugal speed in step N4 is 10000r / min, the centrifugal time is 10min, and the drying process is carried out in a blast drying oven, the drying temperature is 55°C, and the drying time is 24h.

6. A method for preparing a hemp core with antibacterial effect according to any one of claims 1 to 5, characterized in that: The steps include: Step I, 10-20 parts of hemp stalks, 5-10 parts of softener-methyl glucoside, 8-12 parts of antibacterial composition, 8 parts of ethanol, and 6-9 parts of surfactant; Step II, peeling off the bast from the hemp stalks weighed in step I and crushing them to obtain hemp fiber powder; Step III, adding the hemp fiber powder obtained in step II to a mixed solution of ethanol and water, stirring at room temperature, and then filtering to obtain sterilized hemp fiber powder; Step VI, adding softener-methyl glucoside into water and stirring to dissolve, to obtain softener-methyl glucoside aqueous solution, adding the sterilized hemp fiber powder obtained in step III into the softener-methyl glucoside aqueous solution, heating and stirring, and filtering to obtain softened hemp fiber powder; Step V, adding the surfactant weighed in step I to a mixed solution of ethanol and water, heating and stirring to obtain a mixed solution II, adding the softened hemp fiber powder obtained in step VI to the mixed solution II, ultrasonically treating, and then continuing to stir and heat to obtain an adsorbent fiber material mixed solution; Step IV, adding the antibacterial composition to the mixed solution of the adsorbent fiber material obtained in step V, ultrasonically treating it, then continuing to heat and stir it, cooling it to room temperature and letting it stand, filtering and removing the mixed solution to obtain the hemp core material, drying it, and collecting the material to obtain the hemp core with antibacterial effect.

7. The method for preparing a hemp core with antibacterial effect according to claim 6, characterized in that: The mass ratio of hemp fiber powder, ethanol and water in step III is 10-20:8:15, and the stirring time is 30 minutes; the mass ratio of softener-methyl glucoside and water in step VI is 6-8:10, the mass ratio of sterilized hemp fiber powder and softener-methyl glucoside aqueous solution is 10-15:12, the heating temperature is 60-80°C, and the heating time is 30-50 minutes; the mass ratio of surfactant, ethanol and water in step V is 6-9:6:10, the heating temperature is 55-65°C, and the heating time is 2 0-40min, the mass ratio of softened hemp fiber powder and mixed solution II is 6-10:8, the ultrasonic power is 0.5kw, the ultrasonic time is 25-35min, the heating temperature is 60℃, and the heating time is 30min. The mass ratio of the antibacterial composition and the adsorbent fiber material in step IV is 8-12:15, the ultrasonic time is 10-20min, the heating temperature is 60℃, the heating time is 30min, the standing time is 2h, and the drying is carried out in a blast drying oven at a drying temperature of 65℃ and a drying time of 24h.

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