Fluid detection system based on double-fiber optical tweezers and Raman spectrum

Abstract

The invention relates to a fluid detection system based on double-fiber optical tweezers and a Raman spectrum, and belongs to the field of spectrum detection and optical tweezers control. The system comprises a control module, an optical image recognition module, a capture light source, a Raman excitation light source, a substrate, first double-fiber optical tweezers, second double-fiber optical tweezers, a Raman excitation light transmission fiber, a spectrum acquisition fiber and a Raman spectrometer. Wherein the control module is respectively connected with the optical image recognition module and the light source, the capture light source is respectively connected with the first double-fiber optical tweezers and the second double-fiber optical tweezers, the Raman excitation light source is connected with the Raman excitation light transmission fiber, and the Raman spectrometer is connected with the spectrum acquisition fiber. The device can realize automatic recognition, capture and control and Raman detection of particles or cells, and is complete in function, simple in structure and flexible to operate.

Description

Technical Field

[0001] The invention belongs to the field of spectrum detection and optical tweezers control, and relates to a fluid detection system based on double-fiber optical tweezers and Raman spectroscopy. Background Art

[0002] Raman spectroscopy is a spectral technique that uses optical Raman scattering to study molecular vibrations. It can reveal microscopic information such as the sample's morphology, molecular structure, phase state, chemical composition, and crystal state. At present, compared with other detection technologies, Raman spectroscopy has the characteristics of being non-destructive, rapid, simple to prepare samples, and rich in information, and is widely used in various fields such as biomedicine, materials science, chemical analysis, and art archaeology.

[0003] Optical tweezers technology relies on the momentum exchange between light and tiny material particles, which is a mechanical effect. It not only demonstrates the basic property of light having momentum, but also becomes a classic example of the photomechanical effect. The invention of optical tweezers not only expands the boundaries of the field of optics, but also promotes cross-disciplinary cooperation between optics and other disciplines, showing its unique value and importance. Dual-fiber optical tweezers technology is a typical type of optical tweezers technology. It plays an important role in fields such as biomedicine and optical trapping, and has significant potential and broad application prospects for applications such as cell research and particle manipulation.

[0004] Combining the particle or cell capture function of optical tweezers technology with the structure or component analysis function of Raman spectroscopy will greatly expand the application of Raman spectroscopy analysis in various fields. However, the existing optical tweezers capture system has the disadvantages of low capture flexibility for particles or cells and lack of screening of captured particles, which limits the application of Raman spectroscopy analysis. Summary of the invention

[0005] In view of this, an object of the present invention is to provide a fluid detection system based on dual-fiber optical tweezers and Raman spectroscopy to achieve the purposes of automatic identification, capture and control of particles or cells, Raman detection, etc.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] A fluid detection system based on dual-fiber optical tweezers and Raman spectroscopy, the system comprising a control module, an optical image recognition module, a capture light source, a Raman excitation light source, a substrate, a first dual-fiber optical tweezers, a second dual-fiber optical tweezers, a Raman excitation light transmission fiber, a spectrum collection fiber and a Raman spectrometer.

[0008] The substrate surface is etched with a "M"-shaped groove, which includes a first groove for fluid passage, a second groove for fixing a first double-fiber optical tweezers, a third groove for fixing a second double-fiber optical tweezers, and a fourth groove for fixing Raman excitation light transmission optical fiber and spectrum collection optical fiber. The first groove and the second groove should be orthogonally distributed. The substrate shape can be regular shapes such as rectangle, circle, trapezoid, etc., or it can be irregular.

[0009] The optical image recognition module is connected to the control module, and is used to collect image information of the orthogonal region of the first groove and the second groove, and transmit signals to the control module.

[0010] The capture light source input end is connected to the control module, and the output end is connected to the first dual-fiber optical tweezers and the second dual-fiber optical tweezers respectively, for providing the first dual-fiber optical tweezers and the second dual-fiber optical tweezers with the light source required for capture and manipulation; the Raman excitation light source input end is connected to the control module, and the output end is connected to the Raman excitation light transmission optical fiber, for providing Raman spectrum excitation light.

[0011] The first dual-fiber optical tweezers are used to capture a target object in a fluid, and the target object may be a particle or a cell, etc.

[0012] The second dual-fiber optical tweezers are used to perform a rotation operation on the captured target object.

[0013] The Raman excitation light transmission optical fiber is used to transmit the Raman spectrum excitation light to the captured target object, and the spectrum collection optical fiber is used to collect and transmit the Raman scattered light excited by the captured target object.

[0014] The Raman spectrometer is connected to the spectrum collection optical fiber and is used to receive Raman scattered light and perform spectrum analysis.

[0015] Furthermore, the optical image recognition module includes a microscope objective lens, a CCD and an image processing unit. The microscope objective lens is fixed directly above the orthogonal region of the first groove and the second groove, and is used to collect image information of the region; the CCD is used to receive imaging information of the microscope objective lens and transmit it to the image processing unit; the image processing unit identifies whether the target object flows through the region based on the collected image, and identifies whether the target object is captured in the region. The target object can be pre-set as the desired particle or cell, and then the image processing unit identifies whether it is the desired particle or cell.

[0016] The beneficial effects of the present invention are:

[0017] (1) The present invention can realize flexible capture of fluid particles or cells by setting up two dual-fiber optical tweezers, and can also realize special and precise operations on the captured objects.

[0018] (2) The present invention uses an optical image recognition module to achieve the screening, capture and manipulation of specific particles according to user needs.

[0019] (3) The present invention can capture particles or cells while analyzing the structure and composition of the captured particles using Raman spectroscopy. The system is fully functional, simple in structure, and flexible in operation, and is applicable to the research of biological cells and particles.

[0020] Other advantages, objectives and features of the present invention will be described in the following description to some extent, and to some extent, will be obvious to those skilled in the art based on the following examination and study, or can be taught from the practice of the present invention. The objectives and other advantages of the present invention can be realized and obtained through the following description. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to make the purpose, technical solutions and advantages of the present invention more clear, the present invention will be described in detail below in conjunction with the accompanying drawings, wherein:

[0022] Figure 1 It is a dual-fiber optical tweezers structure;

[0023] Figure 2 It is a schematic diagram of the structure of a "M"-shaped optical fiber sheet fluid tank;

[0024] Figure 3 Schematic diagram of the structure of the fiber optic tweezers Raman fluid detection system. DETAILED DESCRIPTION

[0025] The following describes the embodiments of the present invention by specific examples, and those skilled in the art can easily understand other advantages and effects of the present invention from the contents disclosed in this specification. The present invention can also be implemented or applied through other different specific embodiments, and the details in this specification can also be modified or changed in various ways based on different viewpoints and applications without departing from the spirit of the present invention. It should be noted that the illustrations provided in the following embodiments only illustrate the basic concept of the present invention in a schematic manner, and the following embodiments and features in the embodiments can be combined with each other without conflict.

[0026] Among them, the drawings are only used for illustrative explanations, and they only represent schematic diagrams rather than actual pictures, and should not be understood as limitations on the present invention. In order to better illustrate the embodiments of the present invention, some parts of the drawings may be omitted, enlarged or reduced, and do not represent the size of actual products. For those skilled in the art, it is understandable that some well-known structures and their descriptions in the drawings may be omitted.

[0027] The same or similar numbers in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if the terms "upper", "lower", "left", "right", "front", "rear", etc. indicate the orientation or position relationship, they are based on the orientation or position relationship shown in the drawings, which is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operate in a specific orientation. Therefore, the terms describing the position relationship in the drawings are only used for illustrative purposes and cannot be understood as limiting the present invention. For ordinary technicians in this field, the specific meanings of the above terms can be understood according to specific circumstances.

[0028] like Figure 1 The figure shows the structure of dual-fiber optical tweezers. The capture principle is that two laser beams are emitted from oppositely arranged optical fibers. Under the action of the light field, the particles will be bound in the light trap formed by the two laser beams, thereby achieving the capture of the particles. The capture and release of particles can be achieved by regulating the presence or absence of power input into the optical fiber. In addition, by regulating the size of the optical power input into the two optical fibers, the lateral capture position of the particles can also be precisely controlled.

[0029] Based on the above dual-fiber optical tweezers, an embodiment of the present invention provides a fluid detection system based on dual-fiber optical tweezers and Raman spectroscopy, such as Figure 3 As shown, it includes a control module, an optical image recognition module, a capture light source, a Raman excitation light source, a Raman spectrometer, a "M"-shaped fiber sheet fluid tank, a first double-fiber optical tweezers, a second double-fiber optical tweezers, and a Raman excitation light transmission fiber and a spectrum collection fiber. Among them, the optical image recognition module includes a microscope objective, a CCD and an image processing unit.

[0030] The microscope objective lens is arranged directly above the "M"-shaped optical fiber sheet fluid slot, and is used to collect image information of the central area of ​​the fluid slot. The microscope objective lens is imaged on the photosensitive surface of the CCD, and the collected image of the central area of ​​the fluid slot is transmitted to the image processing unit through the CCD. The image processing unit transmits the signal to the control module after processing.

[0031] The capture light source is connected to the control module, and outputs lasers to the first dual-fiber optical tweezers and the second dual-fiber optical tweezers respectively under the modulation of the control module, which is used for capturing and manipulating particles or cells. The Raman excitation light source is connected to the control module, and outputs Raman spectrum excitation light to the Raman excitation light transmission fiber under the modulation of the control module, which is used for Raman spectrum excitation of captured particles or cells.

[0032] The Raman spectrometer is connected to the spectrum collection optical fiber and is used to receive the Raman scattered light signals of particles or cells.

[0033] Among them, the Raman excitation light transmission fiber and the spectrum collection fiber are two sections of optical fibers arranged opposite to each other. The input end of the Raman excitation light transmission fiber is connected to the Raman excitation light source, and the Raman spectrum excitation light is output to the captured particles or cells. The input end of the spectrum collection fiber is used to receive the Raman scattered light excited by the particles or cells, and output it to the Raman spectrometer.

[0034] The "M"-shaped optical fiber sheet fluid groove is as follows Figure 2 As shown, the main body is a rectangular glass substrate, and "M"-shaped grooves are etched on the glass substrate. One of the grooves parallel to the edge of the glass substrate (referred to as the first groove) is used for fluid to pass through, the groove orthogonal to the first groove (referred to as the second groove) is used to fix the first double-fiber optical tweezers, one of the remaining two grooves (referred to as the third groove) is used to fix the second double-fiber optical tweezers, and the last groove (referred to as the fourth groove) is used to fix the Raman excitation light transmission fiber and the spectrum collection fiber.

[0035] The first double-fiber optical tweezers are used to achieve stable capture of cells or particles in the fluid flowing through the first groove. The second double-fiber optical tweezers are constructed in a central axial misalignment mode, which is used to achieve special manipulation functions such as rotation of captured cells or particles.

[0036] The system works as follows:

[0037] The user first inputs the specific particles or cells that are desired to be captured into the control module, and then passes the fluid into the fluid tank. The target type of particles or cells are quickly identified through the optical image recognition module, where the optical image recognition module can recognize the collected images through an artificial neural network.

[0038] When the target particle or cell is identified, the optical image recognition module sends a "capture" signal to the control module. After receiving the signal, the control module controls the capture light source to emit laser light, which is input to the first dual-fiber optical tweezers to achieve stable capture of the target particle or cell. In this process, the input light power in the first dual-fiber optical tweezers can be controlled to achieve lateral position control of the captured particle or cell. At the same time, the control module also synchronously provides light input to the second dual-fiber optical tweezers to achieve precise rotational manipulation of the particles or cells captured by the first dual-fiber optical tweezers, and adjust their position and posture.

[0039] After adjusting the position and posture, the user can control the Raman excitation light source through the control module, and input Raman excitation light into the Raman excitation light transmission optical fiber. The Raman excitation light can excite the Raman scattering spectrum of the captured particles or cells. The excited Raman scattering spectrum is then output to the Raman spectrometer through the spectrum collection optical fiber for spectral analysis and extraction of information such as material structure and composition.

[0040] In another embodiment of the present invention, the first dual-fiber optical tweezers, the second dual-fiber optical tweezers, and the light-guiding optical fibers in the Raman excitation light transmission optical fiber and the spectrum collection optical fiber can be replaced with optical waveguides. Specifically, in the "M"-shaped optical fiber sheet fluid tank, the first dual-fiber optical tweezers, the second dual-fiber optical tweezers, and the light-guiding optical fibers in the Raman excitation light transmission optical fiber and the spectrum collection optical fiber that have been fixed are directly converted into optical waveguides by femtosecond laser modification.

[0041] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solution of the present invention can be modified or replaced by equivalents without departing from the purpose and scope of the technical solution, which should be included in the scope of the claims of the present invention.

Claims

1. A fluid detection system based on dual-fiber optical tweezers and Raman spectroscopy, characterized in that: The system includes a control module, an optical image recognition module, a capture light source, a Raman excitation light source, a substrate, a first double-fiber optical tweezers, a second double-fiber optical tweezers, a Raman excitation light transmission fiber, a spectrum collection fiber and a Raman spectrometer; The substrate surface is etched with "M"-shaped grooves, the "M"-shaped grooves comprising a first groove for allowing fluid to pass through, a second groove for fixing the first double-fiber optical tweezers, a third groove for fixing the second double-fiber optical tweezers, and a fourth groove for fixing the Raman excitation light transmission optical fiber and the spectrum collection optical fiber; The first groove and the second groove are orthogonal; The optical image recognition module is connected to the control module, and is used to collect image information of the orthogonal region between the first groove and the second groove, and transmit a signal to the control module; The capture light source is connected to the control module and is used to provide the first dual-fiber optical tweezers and the second dual-fiber optical tweezers with a light source required for capture and manipulation; The Raman excitation light source is connected to the control module and is used to provide Raman spectrum excitation light to the Raman excitation light transmission optical fiber; The first dual-fiber optical tweezers are connected to the capture light source and are used to capture the target object in the fluid; The second dual-fiber optical tweezers are connected to the capture light source and are used to perform a rotation operation on the captured target object; The Raman excitation light transmission optical fiber is connected to the Raman excitation light source and is used to transmit the Raman spectrum excitation light to the captured target object; The spectrum collection optical fiber is connected to the Raman spectrometer and is used to collect and transmit the Raman scattered light excited by the captured target object; The Raman spectrometer is connected to the spectrum collection optical fiber and is used to receive Raman scattered light and perform spectrum analysis.

2. The fluid detection system according to claim 1, characterized in that: The optical image recognition module includes a microscope objective lens, a CCD and an image processing unit; the microscope objective lens is fixed directly above the orthogonal area of ​​the first groove and the second groove, and is used to collect image information of the area; the CCD is used to receive imaging information of the microscope objective lens and transmit it to the image processing unit; the image processing unit recognizes whether a target object flows through the area based on the collected image, and distinguishes whether the target object is captured in the area.

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