Calcium and magnesium ion sensing detection system based on fluorescence colorimetry

Abstract

The invention discloses a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, relates to the technical field of environment detection, and aims to solve the problems that the traditional calcium and magnesium ion sensing detection is slow in speed and cannot perform real-time detection, and the system comprises a miniature fluorescence light path module and a data acquisition and control module, the micro fluorescent light path module comprises an excitation light source and an optical filter, the excitation light source emits light to the sample cell through the optical filter, and a detector is arranged on one side of the sample cell; the data acquisition and control module comprises a microprocessor connected with the detector, a temperature control module for detecting the temperature of the sample pool and a power supply management module for charging management, and the microprocessor is connected with a communication module. The method can realize rapid and online detection of calcium and magnesium ions in wastewater, has the advantages of short system response time, portability and real-time detection, and has important application value in the fields of environment detection and sensing.

Description

Technical Field

[0001] The present invention relates to the technical field of environmental detection, and in particular to a calcium and magnesium ion sensing detection system based on fluorescence colorimetry. Background Art

[0002] Traditional spectral detection methods such as atomic absorption spectroscopy, ultraviolet visible spectroscopy and fluorescence spectroscopy often have shortcomings such as large equipment size, high price and inability to connect devices, and are not suitable for outdoor measurement, real-time measurement, portable measurement and rapid spectral measurement. Fluorescence colorimetry is a method that uses ion fluorescent probes to quickly and quantitatively detect water samples by colorimetry. It can solve the shortcomings of traditional fluorescence spectroscopy for the determination of calcium and magnesium ions, such as large equipment size and high price. In response to the urgent need for wastewater monitoring and descaling in the coal chemical industry, a calcium and magnesium ion sensing online detection method and system based on fluorescence colorimetry is designed by combining fluorescence colorimetry with Internet of Things technology to achieve rapid and accurate detection of calcium and magnesium ions.

[0003] The Chinese patent with publication number CN114034687A discloses a high-precision calcium ion detection method and device based on electrochemistry, which includes a chemiluminescent flow injection system and an electrochemical external measurement device, but the device is an offline detection device. The related calcium and magnesium ion sensing detection patents do not involve an online detection system based on fluorescence colorimetry. Summary of the invention

[0004] The present invention solves the problem that the traditional calcium and magnesium ion sensing detection speed is slow and real-time detection cannot be performed, and proposes a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, thereby realizing rapid and online detection of calcium and magnesium ions in wastewater.

[0005] In order to achieve the above-mentioned purpose, the present invention adopts the following technical scheme: a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, comprising a miniature fluorescence optical path module and a data acquisition and control module, wherein the miniature fluorescence optical path module comprises an excitation light source and a filter, wherein the excitation light source is emitted to a sample pool through the filter, and a detector is provided on one side of the sample pool; the data acquisition and control module comprises a microprocessor connected to the detector, a temperature control module for detecting the temperature of the sample pool, and a power management module for charging management, wherein the microprocessor is connected to a communication module.

[0006] In the present technical solution, the micro-fluorescence optical path module mainly includes an excitation light source, a filter, a sample cell and a detector, and the data acquisition and control module mainly includes a microprocessor, a temperature control module and a communication module; compared with traditional detection methods, the technical solution of the present invention has the advantages of short system response time, portability and real-time detection, and has important application value in the fields of environmental detection and sensing.

[0007] The present invention is further configured as follows: the micro fluorescent light path module is a dual light path system, the distance between the excitation light source and the filter is less than or equal to 1 cm, and the angle between the excitation light source and the detector receiving light source is 90°.

[0008] In this technical solution, the excitation light source and the filter are placed in sequence along the center, and the distance between the excitation light source and the filter is limited, and the angle between the laser court and the detector receiving the light source is determined.

[0009] The present invention is further configured as follows: the excitation light source is an LED light source, and its excitation power is greater than or equal to 1w; the half bandwidth of the filter is greater than or equal to 3nm, and the diameter of the filter is less than or equal to 5mm.

[0010] In this technical solution, the type of laser light source and the excitation power are determined, and the half-bandwidth and diameter of the filter are determined.

[0011] The present invention is further configured as follows: the sample pool comprises a sample holder and a cuvette, the cuvette is light-transmissive on four sides, and the capacity of the cuvette is less than or equal to 1 mL.

[0012] The cuvette used in the present technical solution is light-transmissive on four sides, and its capacity is less than or equal to 1 mL.

[0013] The present invention is further configured as follows: the sample pool includes an ion probe and a water sample solution, the ion probe is a calcium ion probe or a magnesium ion probe, the calcium ion probe is one of Fluo-3 and Fura-2, the magnesium ion probe is one of Mag-Fura-2 and MagnesiumGreen, the concentration of the ion probe solution is 0.1mM~1mM, and the volume ratio of the ion probe solution to the water sample solution is 1:9~3:7.

[0014] In this technical solution, the probe and the water sample solution of the sample pool are limited.

[0015] The present invention is further configured as follows: a signal conversion unit is provided in the detector, and the signal conversion unit can perform analog and digital signal conversion of the fluorescence signal.

[0016] In the technical solution, the detector in the micro fluorescent light path module can realize analog / digital signal conversion of the fluorescent signal.

[0017] The present invention is further configured such that: the communication module realizes the measurement selection of ion types through data communication, and displays the concentrations of calcium ions and magnesium ions in the water sample to be tested.

[0018] In this technical solution, the communication module not only has a communication function, but also has a human-computer interaction function.

[0019] The present invention is further configured as follows: the thermoelectric cooling device is tightly fitted to the sample pool, the area of ​​the thermoelectric cooling device is less than or equal to 10mm*10mm, and the cooling power of the thermoelectric cooling device is greater than or equal to 2.94W.

[0020] The present invention is further configured as follows: the temperature control module includes a temperature sensor and an adjustment component, and the adjustment component includes a thermoelectric cooling device and a TEC driver connected to the thermoelectric cooling device.

[0021] In this technical solution, temperature regulation is achieved by adjusting components.

[0022] The present invention is further configured such that: the detector is one of an RGB color sensor or a visible spectrum sensor.

[0023] In this technical solution, the detector can be an RGB color sensor or a visible spectrum sensor.

[0024] The present invention can bring the following beneficial effects: The present invention relates to a calcium and magnesium ion sensing detection system based on fluorescence colorimetry. Compared with traditional spectral detection methods such as atomic absorption spectroscopy, ultraviolet visible spectroscopy and fluorescence spectroscopy, the detection methods often have the disadvantages of large equipment size, high price and inability to connect devices, and the complexometric titration colorimetric method has the disadvantages of complex operation and slow system response time. The present invention has the advantages of short system response time, portability and real-time detection, and has important application value in the fields of environmental detection and sensing. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 This is a scene schematic diagram of a calcium and magnesium ion sensing detection system based on fluorescence colorimetry in the present application.

[0026] Figure 2 This is a schematic diagram of the overall framework of a calcium and magnesium ion sensing detection system based on fluorescence colorimetry in the present application.

[0027] Figure 3 This is a schematic diagram of the operation flow of a calcium and magnesium ion sensing detection system based on fluorescence colorimetry in the present application.

[0028] Reference numerals 1. LED light source 2. Filter 3. Sample cell 4. Detector. DETAILED DESCRIPTION

[0029] In order to make the objectives, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific implementation method described herein is only an optimal embodiment of the present invention, which is only used to explain the present invention and does not limit the scope of protection of the present invention. All other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0030] High-salt wastewater with high salt content is often discharged during the coal chemical production process. Usually, various combinations of nanofiltration salt separation process and multi-stage salt separation process combining evaporation concentration-cooling crystallization are used to separate the brine. During the treatment process, scaling ions such as calcium and magnesium ions will form hard scale, causing blockage or even damage to equipment pipelines, thereby causing serious economic losses. Therefore, it is of great significance to realize intelligent monitoring of scaling calcium and magnesium ions in coal chemical wastewater.

[0031] Existing calcium and magnesium ion water quality detection often uses the principles of complexometric titration, ion selective electrode method, atomic absorption spectroscopy, ultraviolet visible spectroscopy or fluorescence spectroscopy for determination. Among them, complexometric titration is a method of determining metal ions by using ligands to form stable complexes with metal ions. Nitrogen has the disadvantages of complex testing process and long system detection time. Ion selective electrode is a method of determining the concentration of corresponding ions based on the reaction electromotive force of different systems by utilizing the selective interaction between electrodes and ions, but it has the disadvantage of poor stability of membrane electrodes.

[0032] Traditional spectral detection methods such as atomic absorption spectroscopy, ultraviolet-visible spectroscopy and fluorescence spectroscopy often have the disadvantages of large equipment size, high price and inability to connect devices. They are not suitable for application scenarios such as outdoor measurement, real-time measurement, portable measurement and rapid spectral measurement.

[0033] Fluorescence colorimetry is a method that uses ion fluorescent probes to quickly and quantitatively detect water samples through colorimetry. It can solve the shortcomings of traditional fluorescence spectroscopy for determining calcium and magnesium ions, which has the disadvantages of large size and high price of equipment.

[0034] In response to the urgent need for wastewater monitoring and descaling in the coal chemical industry, a calcium and magnesium ion sensing detection system based on fluorescence colorimetry was proposed by combining fluorescence colorimetry with Internet of Things technology to achieve rapid and accurate detection of calcium and magnesium ions.

[0035] The Chinese patent with publication number CN114034687A cannot solve the above problems. Compared with the Chinese patent with publication number CN114034687A, the calcium and magnesium ion sensing detection system based on fluorescence colorimetry of the present invention has the advantages of fast detection speed, high integration, portability and real-time detection, and has important application value in the fields of environmental detection and sensing.

[0036] Example 1 This embodiment proposes a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, referring to Figure 1 and Figure 2 It mainly includes a micro-fluorescence optical path module and a data acquisition and control module, wherein the micro-fluorescence optical path module mainly includes an excitation light source and a filter, the laser light source passes through the filter 2 and then shoots to the sample pool 3, and a corresponding detector 4 is arranged on one side of the sample pool; the data acquisition and control module mainly includes a microprocessor, a temperature control module, a communication module and a battery management module, the microprocessor is connected to the communication module, the above-mentioned microprocessor can be connected to the detector, the above-mentioned temperature control module can detect the temperature of the sample pool, and the above-mentioned battery management module can perform charging management.

[0037] In the above technical scheme, the micro-fluorescence optical path module mainly includes an excitation light source, a filter, a sample cell and a detector, and the data acquisition and control module mainly includes a microprocessor, a temperature control module and a communication module; compared with traditional detection methods, the technical scheme of the present invention has the advantages of short system response time, portability and real-time detection, and has important application value in the fields of environmental detection and sensing.

[0038] The micro-fluorescence optical path module is a dual-optical path system. The excitation light source and the filter are placed in sequence along the center. The distance between the excitation light source and the filter is less than or equal to 1 cm. The angle between the excitation light source and the detector receiving the light source is 90°.

[0039] In the above technical solution, the excitation light source and the filter are placed in sequence along the center, and the distance between the excitation light source and the filter is limited, and the angle between the laser court and the detector receiving the light source is determined.

[0040] refer to Figure 1 The excitation light source is an LED light source 1. In the present technical solution, the excitation power of the LED light source is greater than or equal to 1W; the half bandwidth of the filter is greater than or equal to 3nm, and the diameter of the filter is less than or equal to 5mm.

[0041] In the above technical solution, the type of laser light source and the excitation power are determined, and the half-bandwidth and diameter of the filter are determined.

[0042] Furthermore, the sample pool includes a sample holder, a cuvette, an ion probe and a water sample solution.

[0043] The sample pool includes a sample holder and a cuvette. The cuvette is light-transmissive on four sides, and the capacity of the cuvette meets the requirement of being less than or equal to 1 mL.

[0044] The cuvette used in the present technical solution is light-transmissive on four sides, and its capacity is less than or equal to 1 mL.

[0045] For the ion probe and the water sample solution, specifically, the ion probe is a calcium ion probe or a magnesium ion probe, the calcium ion probe is one of Fluo-3 and Fura-2, the magnesium ion probe is one of Mag-Fura-2 and MagnesiumGreen, the concentration of the ion probe solution is 0.1mM~1mM, and the volume ratio of the ion probe solution to the water sample solution is 1:9~3:7.

[0046] In the above technical solution, the probe and the water sample solution of the sample pool are mainly limited.

[0047] A signal conversion unit is provided in the detector, and the main function of the signal conversion unit is to perform analog and digital signal conversion of the fluorescence signal.

[0048] In the above technical solution, the detector in the micro fluorescent light path module can realize analog / digital signal conversion of the fluorescent signal.

[0049] More specifically, the detector may be any one of an RGB color sensor or a visible spectrum sensor.

[0050] The communication module realizes the measurement and selection of ion types through data communication, and displays the concentrations of calcium ions and magnesium ions in the water sample to be tested.

[0051] In the above technical solution, the communication module not only has a communication function, but also has a human-computer interaction function.

[0052] The temperature control module includes a temperature sensor and an adjustment component, and the adjustment component includes a thermoelectric cooling device and a TEC driver connected to the thermoelectric cooling device. In this technical solution, temperature adjustment can be achieved through the adjustment component.

[0053] The thermoelectric cooling device is closely attached to the sample pool, the area of ​​the thermoelectric cooling device is less than or equal to 10 mm*10 mm, and the cooling power of the thermoelectric cooling device is greater than or equal to 2.94 W. The setting of the thermoelectric cooling device further ensures the rapidity and accuracy of temperature adjustment.

[0054] In the present technical solution, the microprocessor in the data acquisition and control module can realize the logic control, data processing and status interaction between the modules; specifically, the above functions can be realized by the logic control unit, data processing unit and status interaction unit inside the microprocessor.

[0055] In this technical solution, the power management module can realize voltage regulation and charging management between modules.

[0056] In this technical solution, the temperature control module can achieve precise control of the temperature of the reaction pool to avoid the influence of temperature changes on the test results.

[0057] In this technical solution, the communication module can realize the measurement and selection of ion types through data communication, as well as human-computer interaction functions such as displaying the concentrations of calcium ions and magnesium ions in the water sample to be tested.

[0058] Example 2 Based on the technical solution described in Example 1, Figure 3 The present embodiment proposes a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, which mainly consists of an excitation light source, a filter, a sample cell and a detector.

[0059] Specifically, based on the above system, the implementation process is as follows: the micro-fluorescence optical path module is a dual optical path system, which performs fluorescence signal detection of calcium ions and magnesium ions respectively. For the fluorescence signal detection of calcium ions, when the probe molecule is selected as Fluo-3, the excitation light source wavelength is a blue LED light source, and its excitation power is 1W, and the filter is a blue narrow-band filter, the half-bandwidth of the blue narrow-band filter is 3nm, and the diameter of the blue narrow-band filter is 5mm. For the fluorescence signal detection of magnesium ions, when the probe molecule is Mag-Fura-2, the excitation light source wavelength is also a blue LED light source, and its excitation power is 1W.

[0060] The filter is a blue narrow-band filter with a half-bandwidth of 3nm and a diameter of 5mm. The excitation light source and the filter are placed in sequence along the center. The distance between the excitation light source and the focusing lens is 1cm, the distance between the focusing lens and the filter is 1cm, and the angle between the excitation light source and the detector receiving light source is 90°. The sample pool consists of a sample holder, a quartz cuvette, an ion probe, and a water sample solution. When the concentration of the calcium or magnesium probe is 1mM, 100μL of the probe molecule solution and 900μL of the wastewater solution are mixed and placed in a quartz cuvette with a volume of 1mL that is transparent on all four sides. The detector specifically uses the TCS34725RGB color sensor, which can receive fluorescence signals and realize analog / digital signal conversion of fluorescence signals.

[0061] The data acquisition and control module is mainly composed of a microprocessor, a power management module, a temperature control module and a communication module. The microprocessor can realize the logic control, data processing and state interaction between the modules; the power management module can realize the voltage adjustment and charging management between the modules; the temperature control module can realize the precise control of the reaction pool temperature to avoid the influence of temperature changes on the test results; the communication module can realize the measurement and selection of ion types through data communication, and display the human-computer interaction functions such as the concentration of calcium ions and magnesium ions in the water sample to be tested.

[0062] Specifically, the microprocessor includes a logic control unit, a data processing unit and a state interaction unit to realize the above functions. The communication module can be flexibly selected according to actual needs.

[0063] In this embodiment, the specific model of the microprocessor is STM32L010R8.

[0064] In this embodiment, the specific model of the power management chip is MP2359 to reduce the voltage to 5V, and LIM1117-3.3 to convert the voltage to 3.3V.

[0065] In this embodiment, the temperature control module is composed of a temperature sensor, a thermoelectric cooling device and a TEC driver, and the thermoelectric cooling device is tightly fitted with the sample pool in the fluorescence optical path system.

[0066] Among them, the specific model of the temperature sensor selected is ds18b20, the size of the thermoelectric cooling device is 10mm*10mm*3.8mm, the cooling power of the thermoelectric cooling device is 2.94W, and the specific model of the TEC driver chip is ADN8834.

[0067] In this embodiment, human-computer interaction with a mobile phone or a computer can be achieved through a communication module such as serial communication, Bluetooth or WiFi.

[0068] Example 3 On the basis of Example 1, this example further proposes a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, which is also composed of an excitation light source, a filter, a sample cell and a detector.

[0069] Based on the above system, the implementation process is as follows: the micro fluorescent light path module is a dual light path system, which can detect the fluorescent signals of calcium ions and magnesium ions respectively.

[0070] For the fluorescence signal detection of calcium ions, when the probe molecule is Fura-2, the excitation light source wavelength is an ultraviolet LED light source, the excitation power is 2W, the filter is an ultraviolet narrow-band filter, the half-bandwidth of the ultraviolet narrow-band filter is 3nm, and the diameter of the ultraviolet narrow-band filter is 4mm.

[0071] For the fluorescence signal detection of magnesium ions, when the probe molecule is MagnesiumGreen, the wavelength of the excitation light source is also a green LED light source, and the excitation power is 2 W. The filter is a green narrow-band filter, the half-bandwidth of the green narrow-band filter is 3nm, and the diameter of the green narrow-band filter is 5mm.

[0072] The excitation light source and the filter are placed in sequence along the center, the distance between the excitation light source and the filter is 0.8 cm, and the angle between the excitation light source and the detector receiving light source is 90°.

[0073] The sample pool consists of a sample holder, a quartz cuvette, an ion probe and a water sample solution. When the concentration of the calcium or magnesium probe is 0.1 mM, 300 μL of the probe molecule solution and 700 μL of the wastewater solution are mixed and placed in a quartz cuvette with a volume of 1 mL and transparent on all four sides.

[0074] In this embodiment, the detector uses an AS7341 visible spectrum color sensor, which can receive fluorescent signals and further realize analog / digital signal conversion of the fluorescent signals.

[0075] The data acquisition and control module is mainly composed of a microprocessor, a power management module, a temperature control module and a communication module. The microprocessor can realize the logic control, data processing and state interaction between the modules; the power management module can realize the voltage adjustment and charging management between the modules; the temperature control module can realize the precise control of the reaction pool temperature to avoid the influence of temperature changes on the test results; the communication module can realize the measurement and selection of ion types through data communication, and display the concentration of calcium ions and magnesium ions in the water sample to be tested.

[0076] In this embodiment, the specific model of the microprocessor is STM32L010R8.

[0077] In this embodiment, the specific model of the power management chip is MP2359 to reduce the voltage to 5V, and LIM1117-3.3 to convert the voltage to 3.3V.

[0078] In this embodiment, the temperature control module is composed of a temperature sensor, a thermoelectric cooling device and a TEC driver, and the thermoelectric cooling device is tightly fitted with the sample pool in the fluorescence optical path system.

[0079] In this embodiment, the specific model of the temperature sensor is ds18b20, the size of the thermoelectric cooling device is 8mm*8mm*4.5mm, and the cooling power of the thermoelectric cooling device is 3.26W.

[0080] In this embodiment, the specific chip model of the TEC driver is ADN8834.

[0081] Example 4 On the basis of Example 1, this example further proposes a calcium and magnesium ion sensing detection system based on fluorescence colorimetry, which is composed of an excitation light source, a filter, a sample cell and a detector.

[0082] Based on the above system, the implementation process is as follows: the micro fluorescent light path module is a dual light path system, which can detect the fluorescent signals of calcium ions and magnesium ions respectively.

[0083] For the fluorescence signal detection of calcium ions, when the probe molecule is selected as Fluo-3, the excitation light source wavelength is a blue LED light source with a power of 1 W, the filter is a blue narrow-band filter with a half-bandwidth of 3 nm and a diameter of 5 mm.

[0084] For the fluorescence signal detection of magnesium ions, when the probe molecule is MagnesiumGreen, the excitation light source wavelength is also a green LED light source, and the power is 1 W. The filter is a blue narrow-band filter, the half-bandwidth of the blue narrow-band filter is 3nm, and the diameter of the blue narrow-band filter is 5mm.

[0085] The excitation light source and the filter are placed in sequence along the center, the distance between the excitation light source and the filter is 1 cm, and the angle direction between the excitation light source and the detector receiving light source is 90°.

[0086] The sample pool consists of a sample holder, a quartz cuvette, an ion probe and a water sample solution. When the concentration of the calcium or magnesium probe is 1 mM, 100 μL of the probe molecule solution and 900 μL of the wastewater solution are mixed and placed in a quartz cuvette with a volume of 1 mL and transparent on all four sides.

[0087] In this embodiment, the detector uses a TCS34725 RGB color sensor, which can receive fluorescence signals and further realize analog / digital signal conversion of the fluorescence signals.

[0088] The data acquisition and control module is mainly composed of a microprocessor, a power management module, a temperature control module and a communication module. The microprocessor can realize the logic control, data processing and state interaction between the modules; the power management module can realize the voltage adjustment and charging management between the modules; the temperature control module can realize the precise control of the reaction pool temperature to avoid the influence of temperature changes on the test results; the communication module can realize the measurement and selection of ion types through data communication, and display the human-computer interaction functions such as the concentration of calcium ions and magnesium ions in the water sample to be tested.

[0089] In this embodiment, the specific model of the microprocessor is STM32L010R8.

[0090] In this embodiment, the specific model of the power management chip is MP2359 to reduce the voltage to 5V, and LIM1117-3.3 to convert the voltage to 3.3V.

[0091] In this technical solution, the temperature control module is composed of a temperature sensor, a thermoelectric cooling device and a TEC driver, and the thermoelectric cooling device is tightly fitted with the sample pool in the fluorescent light path system.

[0092] In this embodiment, the specific model of the temperature sensor selected is ds18b20.

[0093] In this embodiment, the size of the TEC cooling device is 10 mm*10 mm*3.8 mm, and the cooling power of the TEC cooling device is 2.94 W.

[0094] In this embodiment, the specific model of the TEC driver chip is ADN8834.

[0095] In this embodiment, human-computer interaction with a mobile phone or a computer can be achieved through a communication module such as serial communication, Bluetooth or WiFi.

[0096] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the present invention. Although the present invention is described in detail with reference to the embodiments, it should be understood by those skilled in the art that any modification or equivalent replacement of the technical solutions of the present invention does not depart from the spirit and scope of the technical solutions of the present invention and should be included in the scope of the claims of the present invention.

Claims

1. A calcium and magnesium ion sensing detection system based on fluorescence colorimetry, characterized in that: It includes a micro-fluorescence optical path module and a data acquisition and control module. The micro-fluorescence optical path module includes an excitation light source and a filter. The excitation light source is emitted to a sample pool through the filter. A detector is provided on one side of the sample pool. The data acquisition and control module includes a microprocessor connected to the detector, a temperature control module for detecting the temperature of the sample pool, and a power management module for charging management. The microprocessor is connected to a communication module.

2. A calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 1, characterized in that: The micro fluorescent light path module is a dual light path system, the distance between the excitation light source and the filter is less than or equal to 1 cm, and the angle between the excitation light source and the detector receiving light source is 90°.

3. A calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 1 or 2, characterized in that: The excitation light source is an LED light source, and its excitation power is greater than or equal to 1W; the half bandwidth of the filter is greater than or equal to 3nm, and the diameter of the filter is less than or equal to 5mm.

4. A calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 3, characterized in that: The sample pool comprises a sample support and a cuvette, wherein the cuvette is light-transmissive on four sides and has a capacity less than or equal to 1 mL.

5. A calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 1 or 2, characterized in that: The sample pool includes an ion probe and a water sample solution, the ion probe is a calcium ion probe or a magnesium ion probe, the calcium ion probe is one of Fluo-3 and Fura-2, the magnesium ion probe is one of Mag-Fura-2 and Magnesium Green, the concentration of the ion probe solution is 0.1mM~1mM, and the volume ratio of the ion probe solution to the water sample solution is 1:9~3:

7.

6. A calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 1 or 4, characterized in that: The detector is provided with a signal conversion unit, which can perform analog and digital signal conversion of the fluorescence signal.

7. The calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 1 is characterized in that: The communication module realizes the measurement and selection of ion types through data communication, and displays the concentrations of calcium ions and magnesium ions in the water sample to be tested.

8. The calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 4 is characterized in that: The temperature control module includes a temperature sensor and an adjustment component, and the adjustment component includes a thermoelectric cooling device and a TEC driver connected to the thermoelectric cooling device.

9. The calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 8, characterized in that: The thermoelectric cooling device is tightly attached to the sample pool, the area of ​​the thermoelectric cooling device is less than or equal to 10mm*10mm, and the cooling power of the thermoelectric cooling device is greater than or equal to 2.94W.

10. The calcium and magnesium ion sensing detection system based on fluorescence colorimetry according to claim 6, characterized in that: The detector is one of an RGB color sensor or a visible spectrum sensor.

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