Chromatographic detection device

Abstract

The invention discloses a chromatographic detection device which comprises an outer cylinder and an inner core, and the outer cylinder is arranged on the inner core in a sleeving manner; one end of the inner core and the inner wall of one end of the outer cylinder form a sample adding space for accommodating a sample, or one end of the inner core is provided with a sample adding space for accommodating the sample; a plurality of chromatography grooves are formed in the surface of the inner core, extend in the direction away from the sample adding space and are the same in shape; a plurality of liquid outlets are formed in the inner core, each liquid outlet is communicated with the sample adding space, and each chromatography groove is communicated with one liquid outlet; when a sample is contained in the sample adding space, the sample can break through the effect of surface tension and flow out of the liquid outlet; an observation area is arranged at one end, far away from the sample adding space, of the chromatography tank; an observation hole for observing the observation area is formed in the outer cylinder. The chromatographic detection device provided by the invention has the effects of simplicity and convenience in operation, high detection speed and good detection uniformity.

Description

Technical Field

[0001] The invention belongs to the technical field of biological detection, and in particular relates to a chromatography detection device. Background Art

[0002] With the rapid development of modern technology, chromatography technology is often used in the field of in vitro diagnosis to diagnose and test diseases and other items. Chromatographic test cards are important tools in the field of in vitro diagnosis.

[0003] Common chromatography test cards are generally long rectangular structures. During the test, the sample is dripped into the sample well of the test card. The substance to be tested in the sample will move along the chromatography membrane together with the colloidal gold marker and bind to the specific antibody or antigen on the test line. Chromatographic testing has the advantages of simple and fast operation, high sensitivity, low cost, accurate results, and a wide range of applications.

[0004] In actual clinical applications, it is often necessary to test multiple items and hope to obtain the test results of each item at the same time. Currently, there are also multi-link detection cards on the market, and the existing multi-link cards include single-channel multi-link cards and multi-channel multi-link cards.

[0005] Single-channel multi-link cards perform multiple immunochromatographic reactions on the same channel, but this can easily lead to cross-interference between reactions and affect test results.

[0006] The multi-channel multi-link card sets multiple parallel channels on the same substrate. Although it can effectively avoid cross-interference between reactions of various projects, each channel has a sample addition hole, which may have the following disadvantages: ①It is not convenient for the tester to operate, and the sample needs to be added multiple times; ② To test multiple items on the same sample, multiple channels need to be filled first, which requires a large amount of sample; ③ When adding samples through multiple sample holes, the sample addition time is different and the detection uniformity is poor.

[0007] There are also multi-channel multi-link cards on the market that connect multiple channels to the same sample well for use, but there are still the following shortcomings: ① The lengths of multiple channels are inconsistent, the required detection time is different, and the detection uniformity is poor; ② In order to connect multiple channels in parallel with the same sample well, the channel needs to be extended, resulting in a long detection time; ③ The multi-unit card is generally thinner in thickness and has a limited volume of the sample adding hole. When adding the sample through one sample adding hole, the sample often needs to be added dropwise multiple times. This process requires the operator to work for a long time, increasing the workload of the tester. Summary of the invention

[0008] In view of the deficiencies in the prior art, an object of the present invention is to provide a chromatography detection device.

[0009] In order to achieve the above-mentioned invention object, the technical solution adopted by the present invention includes: A chromatography detection device comprises an outer cylinder and an inner core, wherein the outer cylinder is sleeved on the inner core; One end of the inner core and the inner wall of one end of the outer tube form a sample loading space for accommodating the sample, or one end of the inner core is provided with a sample loading space for accommodating the sample; The inner core surface is provided with a plurality of chromatography grooves, the chromatography grooves are at least used to accommodate chromatography paper, the chromatography grooves extend in a direction away from the sample loading space, and the plurality of chromatography grooves have the same shape; the inner core is provided with a plurality of liquid outlets, each of which is connected to the sample loading space, the number of the liquid outlets is equal to and corresponds to the chromatography grooves, and each of the chromatography grooves is connected to one of the liquid outlets; when the sample loading space contains a sample, the sample can break through the surface tension and flow out from the liquid outlet; An observation area is arranged at one end of the chromatography tank away from the sample adding space, and an observation hole for observing the observation area is opened on the outer cylinder.

[0010] Preferably, each of the chromatography tanks extends to the end of the inner core and forms a liquid outlet with the end of the inner core.

[0011] Preferably, each of the chromatography tanks penetrates the sample adding space and forms a liquid outlet with the inner wall of the sample adding space. Preferably, the outer cylinder and the inner core are detachably connected.

[0012] Preferably, the inner core is threadably connected to the outer cylinder.

[0013] In the present invention, the inner core and the outer cylinder are threadedly connected, and the inner core and the outer cylinder are tightly connected. The threaded connection method is conducive to adapting to and overcoming the production errors of the inner core and the outer cylinder, so that the inner core and the outer cylinder can still be tightly connected well when there are production errors. Therefore, the production accuracy of the inner core and the outer cylinder does not need to be particularly strict, which is conducive to reducing the production cost and yield rate of the inner core and the outer cylinder.

[0014] Preferably, the inner core and the outer tube are connected via a snap-fit ​​structure.

[0015] In the present invention, the buckle structure connection method makes the connection and fixing process of the inner core and the outer cylinder extremely simple and fast, with low operation difficulty, which is conducive to reducing the difficulty of learning and using the chromatography detection device.

[0016] Preferably, the chromatography tank is arranged vertically, and the sample adding space is located at the top of the outer cylinder.

[0017] In the present invention, the chromatography tank is arranged vertically, so that the sample can accelerate the chromatography speed and reduce the chromatography difficulty under the action of gravity during the chromatography process, which is beneficial to improving the chromatography rate and improving the chromatography success rate, thereby facilitating the learning and use difficulty of the chromatography detection device.

[0018] Preferably, the surface of the inner core is an inclined surface, and the outer shape of the inner core gradually shrinks from an end away from the sample loading space to an end close to the sample loading space.

[0019] In the present invention, when the chromatography detection device is placed vertically, the overall structure is small at the top and large at the bottom, which is not easy to fall over, which is beneficial to the stability of the placement of the chromatography detection device, thereby improving the practicality of the chromatography detection device.

[0020] Preferably, the inner core surface is a vertical surface.

[0021] Preferably, a base is provided at one end of the inner core away from the sample adding space, or a base is provided at one end of the outer tube away from the sample adding space.

[0022] Preferably, the surface of the inner core is in contact with the inner wall of the outer tube.

[0023] In the present invention, the surface of the inner core fits the inner wall of the outer tube, which is beneficial to improving the stability of the connection between the inner core and the outer tube. At the same time, it can form a barrier between two adjacent chromatography tanks, thereby helping to avoid cross interference between the two adjacent chromatography tanks.

[0024] Preferably, a plurality of chromatography grooves are evenly distributed on the surface of the inner core.

[0025] Preferably, the number of the chromatography tanks is 2 to 6.

[0026] Preferably, it further comprises a sealing plug, wherein the sealing plug has a plug portion, and the plug portion and the sample adding space form a piston structure.

[0027] In the present invention, after the sample is added to the sample adding space, the sealing plug can seal the sample adding space, which is convenient for moving the chromatography detection device and can prevent the sample from continuously contacting with the outside air.

[0028] In the present invention, the sample can be driven to flow toward the liquid outlet by pushing the sealing plug, thereby increasing the chromatography speed of the chromatography detection device; at the same time, when the chromatography detection device is placed slightly tilted, pushing the sealing plug can drive the sample to flow toward the liquid outlet, and active distribution of the sample can be achieved, which is beneficial to improving the uniformity of the detection.

[0029] Compared with the prior art, the advantages of the present invention include: (1) The chromatographic detection device provided by the present invention has a simple structure, low production cost, easy operation, and can perform multiple tests simultaneously; (2) The present invention provides a chromatography detection device. After the sample is added to the sample adding space, it contacts the chromatography paper and starts the chromatography process. The sample can be chromatographically detected without going through the diversion process, and the detection speed is fast. The chromatographic length that the sample needs to go through is the same, and the detection uniformity is good. (3) The volume of the sample loading space of the chromatographic detection device provided by the present invention can be easily designed according to actual needs, so that the required amount of sample in the chromatographic process can be met by only one loading, the operation is simple, and the detection uniformity is good; (4) The present invention provides a chromatographic detection device, in which the sample can be diverted for chromatographic detection without passing through a diversion channel, and the sample does not need to fill the extended diversion channel, thereby helping to reduce the total amount of sample required. BRIEF DESCRIPTION OF THE DRAWINGS

[0030] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, the drawings described below are only some embodiments recorded in the present application. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying creative work.

[0031] Figure 1 This is an overall schematic diagram of a chromatography detection device in Example 1 of the present invention; Figure 2 This is a schematic diagram of the interior of a chromatography detection device in Example 1 of the present invention; Figure 3 This is an overall schematic diagram of a chromatography detection device in Example 2 of the present invention; Figure 4 This is a schematic diagram of an explosion of a chromatography detection device in Example 2 of the present invention; Figure 5 This is a schematic diagram of the interior of a chromatography detection device in Example 3 of the present invention; Reference numerals: 11. Outer cylinder; 12. Observation hole; 13. Sample adding space; 14. Card slot; 21. inner core; 22. chromatography tank; 23. base; 24. card strip; 31. Sealing plug; 32. Plug tail; 33. Plug part. DETAILED DESCRIPTION

[0032] In view of the deficiencies in the prior art, the inventor of this case has proposed the technical solution of the present invention after long-term research and extensive practice. The following will further explain the technical solution, its implementation process and principle, etc. in conjunction with the drawings in the embodiments of this application and specific implementation cases.

[0033] It should be noted that the embodiments described below with reference to the accompanying drawings are exemplary and are only used to explain the present invention, and cannot be understood as limiting the present invention. The described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments in the present invention, the present invention covers any substitution, modification, equivalent method and scheme made on the spirit, principle and scope of the present invention defined by the claims. All other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0034] In the description of the present application, "first", "second", "third" and similar words do not indicate any order, quantity or importance, but are only used to distinguish different components. Similarly, "one" or "an" and other similar words do not indicate a quantity limitation, but indicate the existence of at least one. "Include" or "comprise" and other similar words mean that the elements or objects appearing before "include" or "comprises" include the elements or objects listed after "include" or "comprises" and their equivalents, and do not exclude other elements or objects. "Connect" or "connected" and other similar words are not limited to physical or mechanical connections, but may include electrical connections, whether direct or indirect.

[0035] In the description of the present application, the terms "center", "upper", "lower", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside" and the like indicate positions or positional relationships based on the positions or positional relationships shown in the drawings, and are only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present application. In addition, when positional terms such as both sides, outside, up and down are used, it should be understood that they are only used to facilitate understanding and description, considering that the structure may be facing other positions.

[0036] In the description of this application, unless otherwise clearly specified and limited, the technical or scientific terms used should have the usual meanings understood by persons with general skills in the field to which this application belongs. Terms such as "install", "connect", and "connect" should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, a conflicting connection, or an integrated connection. For ordinary technicians in this field, the specific meanings of the above terms in this application can be understood according to specific circumstances.

[0037] The embodiments of the present invention are intended to introduce and illustrate the structural composition of the chromatographic detection device and the coordination relationship between the various components. Unless otherwise specified, the dimensions, materials and manufacturing processes of the various components in the chromatographic detection device in the embodiments of the present invention can be selected according to specific circumstances and are not specifically limited or described herein.

[0038] Further, in order to make the public have a better understanding of the present invention, some specific details are described in detail in the following detailed description of the present invention. For those skilled in the art, the present invention can be fully understood without the description of these details. Example 1

[0039] See also Figure 1 and Figure 2 A chromatography detection device comprises an outer cylinder 11 and an inner core 21, wherein the outer cylinder 11 is sleeved on the inner core 21, and one end of the inner core 21 and the inner wall of one end of the outer cylinder 11 form a sample adding space 13 for accommodating a sample.

[0040] Specifically, the outer cylinder 11 and the inner core 21 are both vertical, have vertical axes, and are coaxial with each other; after the outer cylinder 11 is sleeved on the inner core 21, the top of the inner core 21 and the top of the outer cylinder 11 form a sample adding space 13.

[0041] Preferably, a base 23 is provided at one end of the inner core 21 away from the sample adding space 13 .

[0042] Specifically, a base 23 is provided at the bottom of the inner core 21 , and the base 23 is conducive to further improving the stability of the chromatographic detection device and reducing the possibility of tipping. In other embodiments, the base 23 can also be provided at the bottom of the outer cylinder 11 .

[0043] Preferably, the outer cylinder 11 and the inner core 21 are detachably connected.

[0044] Further preferably, the inner core 21 is threadably connected to the outer cylinder 11 .

[0045] Specifically, the surface of the inner core 21 is provided with an external thread at the bottom, and the inner wall of the outer tube 11 is provided with an internal thread matching the external thread at the bottom, so that the inner core 21 and the outer tube 11 can be threadedly connected to each other, and when the inner core 21 and the outer tube 11 are connected to each other, the top of the inner core 21 and the top of the outer tube 11 form a sample adding space 13.

[0046] Preferably, it further comprises a sealing plug 31 , wherein the sealing plug 31 has a plug portion 33 , and the plug portion 33 and the sample adding space 13 form a piston structure.

[0047] Specifically, the sealing plug 31 includes a plug portion 33 and a plug tail 32 that are connected to each other, wherein the plug portion 33 matches the inner wall shape of the top end of the outer tube 11, so that the plug portion 33 can form a seal on the sample loading space 13; and the plug portion 33 has a length in the vertical direction, so that the plug portion 33 can move up and down in the sample loading space 13 and form a piston structure with the sample loading space 13.

[0048] In this embodiment, a plurality of chromatography grooves 22 are provided on the surface of the inner core 21. The chromatography grooves 22 are at least used to accommodate chromatography paper. The chromatography grooves 22 extend in a direction away from the sample loading space 13, and the shapes of the plurality of chromatography grooves 22 are the same. An observation area is provided at one end of the chromatography groove 22 away from the sample loading space 13, and an observation hole 12 for observing the observation area is opened on the outer cylinder 11.

[0049] Specifically, in other embodiments, a protective film covering the observation hole 12 may also be attached to the outer surface of the outer tube 11 to protect the observation area.

[0050] Preferably, the chromatography tank 22 is arranged vertically, and the sample adding space 13 is located at the top of the outer cylinder 11 .

[0051] Further preferably, the surface of the inner core 21 is an inclined surface, and the outer shape of the inner core 21 gradually shrinks from an end away from the sample adding space 13 to an end close to the sample adding space 13 .

[0052] More preferably, the surface of the inner core 21 is in contact with the inner wall of the outer tube 11 .

[0053] Specifically, the outer cylinder 11 and the inner core 21 are both conical in shape, and a plurality of chromatography tanks 22 extend along a generatrix respectively; the inner wall of the outer cylinder 11 and the surface shape of the inner core 21 match each other, and when the inner core 21 and the outer cylinder 11 are connected to each other, the outer cylinder 11 and the inner core 21 fit each other.

[0054] Preferably, a plurality of chromatography grooves 22 are evenly distributed on the surface of the inner core 21 .

[0055] Preferably, the number of chromatography tanks 22 is 2 to 6.

[0056] Specifically, in this embodiment, the number of the chromatography slots 22 is 5, and the 5 chromatography slots 22 are evenly distributed on the surface of the inner core 21 around the axis of the inner core 21. In other embodiments, the number of the chromatography slots 22 may also be 3 or 4.

[0057] In other embodiments, when the number of chromatography slots 22 exceeds 6, the chromatography slots 22 on the surface of the inner core 21 will be too densely arranged, or the volume of the chromatography detection device will increase. In addition, too many chromatography slots 22 will result in a large number of chromatography papers that need to be tested, and some chromatography papers will wait too long for testing.

[0058] In other embodiments, the outer cylinder 11 and the inner core 21 may be cylindrical as a whole, the surface of the inner core 21 is a vertical surface, and the plurality of chromatography tanks 22 extend in the vertical direction.

[0059] In this embodiment, the inner core 21 is provided with a plurality of liquid outlets, each of which is connected to the sample loading space 13, and the number of liquid outlets and the number of chromatography slots 22 are equal and correspond one to one, and each chromatography slot 22 is connected to a liquid outlet; when the sample loading space 13 contains a sample, the sample can break through the surface tension and flow out from the liquid outlet; Preferably, each chromatography tank 22 extends to the end of the inner core 21 and forms a liquid outlet with the end of the inner core 21 .

[0060] Specifically, for any chromatography tank 22, the chromatography tank 22 extends to the top of the inner core 21 and is connected to the sample adding space 13, and the chromatography tank 22 forms a liquid outlet at the top of the inner core 21; when the sample is added to the sample adding space 13, the sample can flow into the chromatography tank 22 from the liquid outlet under the action of gravity and contact the chromatography paper.

[0061] In this embodiment, when assembling the chromatography detection device, the chromatography paper is first loaded into the chromatography tank 22, and then the outer tube 11 and the inner core 21 are connected to each other, the sealing plug 31 is partially inserted into the sample addition space 13, and then the base 23 is placed on a horizontal operating table to complete the assembly of the chromatography detection device.

[0062] In this embodiment, when adding a sample to the chromatography detection device, the sealing plug 31 is first opened, the sample is added into the sample adding space 13, and then the sealing plug 31 is inserted into the sample adding space 13, and finally the plug tail 32 is pushed to push the plug portion 33 into the sample adding space 13, and the sample adding process is completed. After the chromatography process is completed, the result of the sample chromatography can be observed or detected through the observation hole 12. Example 2

[0063] The difference between this embodiment and the first embodiment is that a sample loading space 13 for accommodating a sample is disposed at one end of the inner core 21 , and each chromatography slot 22 penetrates the sample loading space 13 and forms a liquid outlet with the inner wall of the sample loading space 13 .

[0064] Specifically, the top of the inner core 21 is flush with the top of the outer tube 11 , and the sample adding space 13 is arranged at the top of the inner core 21 .

[0065] Further preferably, the inner core 21 and the outer tube 11 are connected via a snap-fit ​​structure.

[0066] Specifically, a protruding clamping strip 24 is provided on the surface of the inner core 21 , and a clamping groove 14 corresponding to the position of the clamping strip 24 is provided on the surface of the outer cylinder 11 , so that the inner core 21 can be clamped and fixed by the clamping strip 24 .

[0067] Specifically, a clamping strip 24 is provided between every two adjacent chromatography slots 22 on the surface of the inner core 21 , and a corresponding number of clamping slots 14 are provided on the surface of the outer cylinder 11 .

[0068] When assembling the chromatography detection device, push the inner core 21 into the outer cylinder 11 and align the clamping strip 24 with the clamping slot 14. After the clamping strip 24 is clamped into the clamping slot 14, the outer cylinder 11 and the inner core 21 can be connected and fixed to each other; partially insert the sealing plug 31 into the sample adding space 13, and then place the base 23 on a horizontal operating table to realize the assembly of the chromatography detection device. Example 3

[0069] The difference between this embodiment and embodiment 2 is that the outer surface of the top of the inner core 21 is provided with an outer thread, the inner surface of the top of the outer cylinder 11 is provided with a corresponding inner thread, and the inner core 21 and the outer cylinder 11 are connected and fixed to each other by threads.

[0070] Specifically, the external thread is arranged on the outer wall of the sample adding space 13 .

[0071] It should be understood that the above embodiments are only for illustrating the technical concept and features of the present invention, and their purpose is to enable people familiar with this technology to understand the content of the present invention and implement it accordingly. It cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, some simple deductions or substitutions can be made without departing from the concept of the present invention. All equivalent changes or modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims

1. A chromatography detection device, characterized in that: It comprises an outer cylinder (11) and an inner core (21), wherein the outer cylinder (11) is sleeved on the inner core (21); One end of the inner core (21) and the inner wall of one end of the outer cylinder (11) form a sample loading space (13) for accommodating a sample, or one end of the inner core (21) is provided with a sample loading space (13) for accommodating a sample; The surface of the inner core (21) is provided with a plurality of chromatography grooves (22), the chromatography grooves (22) being used to accommodate chromatography paper at least, the chromatography grooves (22) extending in a direction away from the sample loading space (13), and the plurality of chromatography grooves (22) are of the same shape; the inner core (21) is provided with a plurality of liquid outlets, each of the liquid outlets being connected to the sample loading space (13), the number of the liquid outlets and the chromatography grooves (22) being equal and corresponding one to one, and each of the chromatography grooves (22) being connected to one of the liquid outlets; when a sample is contained in the sample loading space (13), the sample can break through the effect of surface tension and flow out from the liquid outlet; An observation area is provided at one end of the chromatography tank (22) away from the sample loading space (13), and an observation hole (12) for observing the observation area is provided on the outer cylinder (11).

2. A chromatography detection device according to claim 1, characterized in that: Each of the chromatography tanks (22) extends to the end of the inner core (21) and forms a liquid outlet with the end of the inner core (21); Alternatively, each of the chromatography tanks (22) penetrates the sample loading space (13) and forms a liquid outlet with the inner wall of the sample loading space (13).

3. A chromatography detection device according to claim 1, characterized in that: The outer cylinder (11) and the inner core (21) are detachably connected.

4. A chromatography detection device according to claim 3, characterized in that: The inner core (21) is threadedly connected to the outer cylinder (11); Alternatively, the inner core (21) and the outer tube (11) are connected via a snap-fit ​​structure.

5. A chromatography detection device according to claim 1, characterized in that: The chromatography tank (22) is arranged vertically, and the sample adding space (13) is located at the top of the outer cylinder (11).

6. A chromatography detection device according to claim 5, characterized in that: The surface of the inner core (21) is an inclined surface, and the outer shape of the inner core (21) gradually shrinks from an end away from the sample loading space (13) to an end close to the sample loading space (13); Alternatively, the surface of the inner core (21) is a vertical surface.

7. A chromatography detection device according to claim 5, characterized in that: A base (23) is provided at one end of the inner core (21) away from the sample loading space (13), or a base (23) is provided at one end of the outer cylinder (11) away from the sample loading space (13).

8. A chromatography detection device according to claim 1, characterized in that: The surface of the inner core (21) is in contact with the inner wall of the outer cylinder (11); and / or, a plurality of chromatography grooves (22) are evenly distributed on the surface of the inner core (21); And / or, the number of the chromatography tanks (22) is 2 to 6.

9. A chromatography detection device according to claim 1, characterized in that: The invention also comprises a sealing plug (31), wherein the sealing plug (31) has a plug portion (33), and the plug portion (33) forms a piston structure with the sample adding space (13).

Images