Natural-source plant composition capable of efficiently controlling oil and resisting pollution and application of natural-source plant composition
Through the composition of fermented filtrate of the fermented fruit and the Miroko leaf/stem extract, the problem of difficult to develop natural-derived plant compositions in the prior art that both oil control and pollution-resistant functions is achieved, and efficient oil control and resistance to the The effect of pollution.
Patent Information
- Application Number
- CN202411099712.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-08-12
- Publication Date
- 2025-02-07
AI Technical Summary
It is difficult to develop a natural-derived plant composition that has both oil control and pollution resistance.
The composition of the fermentation filtrate of the fermentation filtrate and the Miloko leaf/stem extract was prepared by a specific extraction and fermentation process, with a mass ratio of 3:1 to 1:3.5.
This composition can effectively inhibit the expression of sebaceous gland cells, significantly reduce the secretion of scalp oil, and has anti-contamination and tensile breakage effects.
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Figure CN119385883A_ABST
Abstract
Description
Technical Field
[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a natural plant composition with high oil control and anti-pollution properties and application thereof. Background Art
[0002] Staying up late at night and having irregular work and rest schedules can cause abnormal scalp conditions. The most obvious manifestation is severe oiliness of the hair. On the other hand, ultraviolet rays, smoking, haze particles, oxidative species, etc. can also pollute the hair, causing it to become dry and brittle and lose elasticity.
[0003] Plant compositions are active functional components of natural origin, which are milder than chemically synthesized components. The development of active functional components of natural origin is a current research hotspot. How to prepare a plant composition of natural origin that has both oil control and anti-pollution functions is a technical problem to be solved. Summary of the invention
[0004] The purpose of this section is to summarize some aspects of embodiments of the present invention and to briefly introduce some preferred embodiments.
[0005] As one aspect of the present invention, the present invention provides a natural plant composition with high oil control and anti-pollution properties, wherein: it is composed of soapberry fruit fermentation filtrate and mirabilis leaf / stem extract, and the mass ratio of soapberry fruit fermentation filtrate to mirabilis leaf / stem extract is 3:1 to 1:3.5.
[0006] The INCI name of the soapberry fruit fermentation filtrate of the present invention is Sapindus mukorossi fruit extract and lactobacillus fermentation product. The INCI name of the Myrothamnus flavescentis leaf / stem extract is Myrothamnus flavescentis leaf / stem extract.
[0007] The natural plant composition with high efficiency in oil control and anti-pollution properties described in the present invention is characterized in that the preparation method of the soapberry fruit fermentation filtrate comprises the steps of crushing the soapberry fruit, adding water, extracting at 60-80° C., filtering, decolorizing, filtering to obtain a soapberry fruit extract, adding Lactobacillus p / antarum for fermentation, sterilizing, and filtering to obtain a soapberry fruit fermentation filtrate.
[0008] Specifically, the soapberry fruit fermentation filtrate is obtained by fermenting soapberry fruit. The extraction method is as follows: the soapberry fruit is crushed and sieved through a 40-120 mesh sieve, deionized water is added at a solid-liquid ratio of 1:3-6, and extracted at 60-80°C for 2-4 hours. The crude extract is filtered through a 1000-2000 mesh filter bag to obtain the crude extract, which is then decolorized with a decolorizer and filtered through a 100-450nm filter membrane to obtain the soapberry fruit extract. Lactobacillus plantarum is added to the soapberry fruit extract, and the extract is fermented at 37°C for 4 days. The extract is then sterilized at 110°C under high pressure and filtered through a 100-450nm filter membrane to obtain the soapberry fruit fermentation filtrate.
[0009] As a preferred embodiment of the natural plant composition with high-efficiency oil control and anti-pollution properties described in the present invention: the preparation method of the Mirova leaf / stem extract comprises the steps of crushing the Mirova leaf / stem and adding it to a butanediol aqueous solution, extracting at 60-80°C, concentrating and filtering to obtain the Mirova leaf / stem extract; the mass concentration of the butanediol aqueous solution is 7-13%.
[0010] An example of the preparation method of the Mirova leaf / stem extract: The Mirova leaves are crushed and passed through a 40-120 mesh sieve, added to a mixed solvent of Mirova leaf: butanediol: water = 1:1:10 by mass ratio for extraction, extracted at 60-80°C for 2-5h, then the extract is cooled to 25-45°C, impurities are filtered out to obtain a crude extract, the crude extract is concentrated to half of the original volume, and then filtered with a 100-450nm filter membrane to obtain the Mirova leaf / stem extract.
[0011] Beneficial effects of the present invention: The present invention studies the effects of soapberry fermentation filtrate and Milo leaf / stem extract on sebaceous gland cells. The experimental results show that soapberry fermentation filtrate and Milo leaf / stem extract can inhibit the expression of sebaceous gland cells, and it is found that the combination of soapberry fermentation filtrate and Milo leaf / stem extract has a synergistic effect on inhibiting the expression of sebaceous gland cells; this study also found that the two have a strong synergistic effect at a mass ratio of 3:1, and have a synergistic effect of inhibiting oil secretion in the mass ratio range of 3:1 to 1:3.5. The present invention finds that soapberry fermentation filtrate and Milo leaf / stem extract can be used in functional products with high-efficiency oil control, and have a good application prospect. In the present invention, Milo leaf / stem extract is extracted by mixing water and butylene glycol. The activity of Milo leaf / stem extract extracted by this method is better than that of water extract or alcohol extract. The combination with soapberry fermentation filtrate has the effect of synergistically inhibiting the expression of sebaceous gland cells, anti-breaking and anti-pollution of hair. BRIEF DESCRIPTION OF THE DRAWINGS
[0012] In order to more clearly illustrate the technical solution of the embodiment of the present invention, the following briefly introduces the drawings required for describing the embodiment, wherein:
[0013] Figure 1 Comparison of reduction in oil secretion was tested on volunteers.
[0014] Figure 2 Data comparison of the inhibition of cellular lipid secretion by samples in different ratios.
[0015] Figure 3 The work-to-break data for different experimental schemes.
[0016] Figure 4 Tensile strength data for different experimental schemes. Figure 5 Take a picture of the hair strand for the microscope. DETAILED DESCRIPTION
[0017] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the specific implementation methods of the present invention are described in detail below in conjunction with specific embodiments.
[0018] Preparation of soapberry fruit fermentation filtrate: After the soapberry fruit is crushed, deionized water is added according to the solid-liquid ratio of 1:5, and extracted at 70°C for 4 hours. The crude extract is filtered through a 1000-mesh filter bag to obtain the crude extract, which is then decolorized with a decolorizer and filtered through a 450nm filter membrane to obtain the soapberry fruit extract. Lactobacillus plantarum is picked up with an inoculation loop and added to the soapberry fruit extract. The extract is fermented at 37°C for 4 days, then sterilized under high pressure at 110°C, and filtered through a 220nm filter membrane to obtain the soapberry fruit fermentation filtrate.
[0019] Preparation of Mirova leaf / stem extract: The Mirova leaves were crushed and passed through an 80-mesh sieve, added to a mixed solvent of Mirova leaf: butanediol: water = 1:1:10 by mass ratio, extracted at 70°C for 4 hours, then the extract was cooled to 25°C, impurities were filtered out to obtain a crude extract, the crude extract was concentrated to half of the original volume, and then filtered with a 450nm filter membrane to obtain the Mirova leaf / stem extract.
[0020] Table 1 Highly effective oil control essence formula
[0021]
[0022] Different essences were prepared according to the above ratios, and the oil-control effect was tested using the consumer experiment method.
[0023] (1) Experimental equipment: Meibometer MB560 hair oil tester (CK Company, Germany)
[0024] (2) Experimental principle: Based on the principle of photometry, the special tape absorbs the oil on the human skin and becomes a translucent tape. Its light transmittance changes, thereby measuring the oil content on the skin surface. The greater the light transmittance of the tape, the more oil on the skin surface it absorbs.
[0025] (3) Measurement parameters: Sebum index T (μg / cm 2 )
[0026] (4) Specific test method: Recruit qualified volunteers (with initial scalp oil content of about 100 mg / cm2 and sebum index ≥ 450 μg / cm2) 2 ) were randomly divided into 6 groups, each with 10 people. The volunteers were asked to try the essence of Example 1 and Comparative Examples 1 to 4 every night, and evenly dripped them on the right scalp. The control group was tried on the left scalp for a total of four weeks. The scalp oil content before, one week, two weeks and four weeks was tested and recorded, and the average oil content of the 10 people was calculated. Before each measurement, the volunteers were asked to sit quietly in a constant temperature and humidity room for 30 minutes, and then tested after stabilization. Finally, the reduction in scalp oil four weeks before and after was compared.
[0027] Table 26 Reduction in scalp oil content of volunteers in four weeks Unit: (μg / cm 2 )
[0028]
[0029] Figure 1 This is the average reduction in fat in volunteers over four weeks. Note: * indicates p<0.05, ** indicates p<0.01, and ** indicates p<0.001.
[0030] The inventor unexpectedly found in the research that adding the essence of soapberry fermentation filtrate and Mironia sibiricum leaf / stem extract at the same time can significantly reduce the oil secretion of consumers' scalp. Consumers who use Example 1 can reduce the oil secretion of scalp by 321.1 μg / cm in four weeks. 2 The combination of the fermented filtrate of the fruit of the Chinese thorn tree or the extract of the leaf / stem of the Chinese thorn tree has the best synergistic effect and has a highly effective oil control effect.
[0031] Sebocyte lipid inhibition rate test:
[0032] Test method: After the cryopreserved human sebaceous gland SZ95 cells were revived, they were incubated with 10% fetal bovine serum, 1×10 5 The cells were cultured in a DMEM high-glucose medium containing 100 mg / L penicillin and 100 mg / L streptomycin at 37°C in a cell culture incubator with 5% carbon dioxide. When the cells adhered to the wall and the confluence exceeded 80%, they were digested and passaged with trypsin containing 0.25% EDTA, and cells in the logarithmic growth phase were used for experiments.
[0033] SZ95 cells in the logarithmic growth phase were digested and counted, and the cell suspension was adjusted to 1×10 4 / mL, transfer 100μL per well to each sample well of a black bottom transparent 96-well plate. The next day, aspirate the culture medium in the well plate and rinse once with PBS. Then add the composition ratio of experiments 1-5. After incubation for 48h, discard the DM EM complete culture medium, carefully rinse twice with PBS, add 10μg / mL Nile red, and let stand in a dark environment at 37℃ for 15min. Use a multifunctional microplate reader with fluorescence function to detect the released fluorescence with an excitation wavelength of 485nm and an absorption wavelength of 565nm, with 3 replicates in each group. Calculate the inhibition rate of lipid synthesis of SZ95 cells in experiments 1-5 according to the following formula.
[0034] Table 3 Sebaceous cell test components
[0035] Experiment number Components (mass ratio) 1 Soapberry fruit fermentation filtrate 2 Milo leaf / stem extract 3 Soapberry fruit fermentation filtrate: Milo leaf / stem extract = 1:1 4 Soapberry fruit fermentation filtrate: Milo leaf / stem extract = 3:1 5 Soapberry fruit fermentation filtrate: Milo leaf / stem extract = 1:3.5
[0036] Experiment 1 was the soapberry fermentation filtrate group, and the concentrations of the soapberry fermentation filtrate were prepared at 1μg / mL, 2μg / mL, 4μg / mL, 8μg / mL, and 16μg / mL respectively; Experiment 2 was the Myrtle leaf / stem extract group, and the concentrations of the Myrtle leaf / stem extract were prepared at 1μg / mL, 2μg / mL, 4μg / mL, 8μg / mL, and 16μg / mL respectively; Experiment 3 was the soapberry fermentation filtrate and the Myrtle leaf / stem extract were mixed in a mass ratio of 1:1, and the total concentrations of the soapberry fermentation filtrate and the Myrtle leaf / stem extract were 1μg / mL, 2μg / mL, 4μg / mL, g / mL, 8μg / mL, 16μg / mL; Experiment 4 was a combination of soapberry fermentation filtrate and Milo leaf / stem extract in a mass ratio of 3:1, and the total concentrations of soapberry fermentation filtrate and Milo leaf / stem extract were 1μg / mL, 2μg / mL, 4μg / mL, 8μg / mL, 16μg / mL; Experiment 5 was a mixture of soapberry fermentation filtrate and Milo leaf / stem extract in a mass ratio of 1:3.5, and the total concentrations of soapberry fermentation filtrate and Milo leaf / stem extract were 1μg / mL, 2μg / mL, 4μg / mL, 8μg / mL, 16μg / mL.
[0037] The above test method was used to obtain the inhibition rate of sebaceous gland cell lipids in different experimental schemes. The data were normalized and the Cl value was calculated using CompuSyn software. A Cl value of 0.1 to 0.3 indicated a strong synergistic effect, and a Cl value of 0.3 to 0.7 indicated a synergistic effect. The statistical analysis results are shown in Table 4.
[0038] Table 4 Sebaceous gland cell lipid inhibition rate in different experimental schemes
[0039]
[0040] Tensile strength test:
[0041] Hair source: healthy black hair from Asian women.
[0042] Test environment: smoke chamber: PM2.5 / 10 concentration is 600μg / m 3 , coal tar 10 mg / cigarette, cigarette consumption 40 cigarettes / 6 hours.
[0043] Test method: Take four groups of healthy black hair, 3 hair bundles in each group; first rinse the hair bundles with 10% SDS solution for 1 minute, then rinse with deionized water for 2 minutes, and pre-treat the hair bundles. Then treat the blank group and experimental groups 1-3 respectively, the treatment method is as follows: first apply the group solution to the surface of the hair bundles for 1 minute, then rinse with deionized water for 2 minutes, after doing this 10 times, blow dry the hair bundles, and put the hair bundles in the smoke chamber for 12 hours, test the breaking work and tensile strength of the hair bundles, the results are as follows Figure 3 , Figure 4 shown.
[0044] Table 5 Tensile strength test experimental grouping
[0045]
[0046]
[0047] Depend on Figure 3 , Figure 4 As shown in the data, after exposure to cigarette smoke, the work to break and tensile strength of the blank control group's hair were significantly lower than those of the experimental group, and the data of the experimental group were better than those of the blank group, indicating that both the soapberry fermentation filtrate and the mirabilis leaf / stem extract have anti-pollution effects, and when the two are used in a 3:1 combination, the anti-pollution effect is more obvious.
[0048] Protein loss test:
[0049] Hair source: healthy black hair from Asian women.
[0050] Test environment: UV light room: UV light 365nm
[0051] Test method: Take four groups of healthy hair bundles, three bundles in each group; prepare four different essences according to the formula in Table 6, and evenly apply the blank group and the formula of experimental group 4-6 to each group of hair bundles for 2 minutes, blow dry with cold air, and then put them into the UV light room for 6 hours; take them out and repeat the above steps for a total of 3 times, and use an electron microscope to take pictures of the surface conditions of the four groups of hair bundles, such as Figure 5 .
[0052] Table 6 Anti-pollution serum formula
[0053]
[0054] Depend on Figure 5 It can be seen that the blank group had a large area of damage after continuous sample loading, while the hair damage was improved after adding the essence of Sapindus mukorossi fermentation filtrate, Milo leaf / stem extract or the combination of the two. In particular, experimental group 6 had the least damage.
[0055] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the spirit and scope of the technical solutions of the present invention, which should all be included in the scope of the claims of the present invention.
Claims
1. A natural plant composition with high oil control and anti-pollution properties, characterized by: The invention is composed of soapberry fruit fermentation filtrate and miltiorrhiza leaf / stem extract, and the mass ratio of the soapberry fruit fermentation filtrate to the miltiorrhiza leaf / stem extract is 3:1 to 1:3.
5.
2. The natural plant composition with high oil control and anti-pollution properties according to claim 1, characterized in that: The preparation method of the soapberry fruit fermentation filtrate comprises the steps of crushing the soapberry fruit, adding water, extracting at 60-80° C., filtering, decolorizing, filtering to obtain a soapberry fruit extract, adding lactobacillus plantarum for fermentation, sterilizing, and filtering to obtain a soapberry fruit fermentation filtrate.
3. The natural plant composition with high oil control and anti-pollution properties according to claim 2, characterized in that: The soapberry fruit is crushed and then added with water, with a solid-liquid ratio of 1:3-6, and the extraction is performed at 60-80° C. and then filtered, and the extraction time is 2-4 hours.
4. The natural plant composition with high oil control and anti-pollution properties according to claim 2 or 3, characterized in that: The fermentation temperature is 37-40° C. and the fermentation time is 4-5 days.
5. The natural plant composition with high oil control and anti-pollution properties according to claim 2 or 3, characterized in that: The method of obtaining the soapberry fruit fermentation filtrate after filtration includes filtering with a 100-450nm filter membrane.
6. The natural plant composition with high oil control and anti-pollution properties according to claim 1, characterized in that: The preparation method of the Milo leaf / stem extract comprises the following steps: crushing the Milo leaf / stem, adding the crushed Milo leaf / stem into a butanediol aqueous solution, extracting at 60-80° C., concentrating, and filtering to obtain the Milo leaf / stem extract; the mass concentration of the butanediol aqueous solution is 7-13%.
7. The natural plant composition with high oil control and anti-pollution properties according to claim 6, characterized in that: The extraction time is 2-5h.
8. The natural plant composition with high oil control and anti-pollution properties according to claim 6, characterized in that: The filtration after concentration includes filtration with a 100-450 nm filter membrane.
9. Use of the natural plant composition with high oil control and anti-pollution properties as claimed in claim 1 in cosmetics.
10. The use according to claim 9, characterized in that: The cosmetics include shampoo products, hair care products or scalp care products.