Composite cannabidiol essential oil composition capable of resisting depression, soothing nerves and helping sleep and preparation method of composite cannabidiol essential oil composition

Abstract

The invention provides a compound cannabidiol essential oil composition for resisting depression, soothing nerves and helping sleep and a preparation method thereof, and belongs to the technical field of depression resistance. The mosquito-repellent incense is prepared from the following raw materials in parts by weight: 5-7 parts of lavender, 3-5 parts of clove, 1-2 parts of folium artemisiae argyi, 4-6 parts of chamomile, 5-7 parts of bergamot, 3-5 parts of verbena blue, 2-4 parts of valeriana affinis, 0.01-0.02 part of a penetration enhancer and 0.05-0.1 part of cannabidiol. The prepared composite cannabidiol essential oil composition for resisting depression, soothing nerves and helping sleep is safe to use, is extracted from flowers and plants, and has very good effects on relieving human mood, relieving pressure and improving insomnia and has very good effects on depression and insomnia.

Description

Technical Field

[0001] The present invention relates to the technical field of anti-depression, and in particular to a composite cannabidiol essential oil composition for anti-depression, tranquilizing the mind and promoting sleep, and a preparation method thereof. Background Art

[0002] With the rapid development of society, people's work and life pressures are getting greater and greater, causing many people to be nervous, palpitating, mentally confused, restless, unable to have high-quality sleep, and even insomnia. Insomnia can cause fatigue, anxiety, general discomfort, listlessness, slow reaction, headache, and inability to concentrate. In severe cases, it can lead to functional diseases such as schizophrenia, depression, anxiety, and autonomic dysfunction.

[0003] Although there are many types of drugs with hypnotic effects, the main purpose of most of them is not to treat insomnia. Currently, the drugs used in the clinical treatment of insomnia mainly include benzodiazepine receptor agonists (BZRAs), melatonin receptor agonists, and antidepressants with hypnotic effects. Although antihistamines (such as diphenhydramine), melatonin, and valerian extracts have hypnotic effects, the existing clinical research evidence is limited and they are not suitable for routine use as insomnia medications. General treatment recommendations: eszopiclone, zolpidem, zolpidem-CR, and zopiclone. The drugs for treating insomnia are complex and numerous, including estazolam, flurazepam, quazepam, temazepam, triazolam, alprazolam, chlordiazepoxide, diazepam, lorazepam, midazolam, zolpidem, zolpidem-CR, zopiclone, eszopiclone and zaleplon, ramelteon, agomelatin, tricyclic antidepressants, selective serotonin reuptake inhibitors (SSRIs), serotonin and norepinephrine reuptake inhibitors (SNRIs), low-dose mirtazapine, low-dose trazodone, etc. Since some drugs have strong side effects and drug dependence, long-term use is generally not recommended. Summary of the invention

[0004] The purpose of the present invention is to provide a composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep and a preparation method thereof. The composition is safe to use, extracted from flowers and plants, and has good effects on soothing human emotions, relieving stress and improving insomnia, and has good effects on depression and insomnia.

[0005] The technical solution of the present invention is achieved in this way: The invention provides a composite cannabidiol essential oil composition for anti-depression, tranquilizing nerves and promoting sleep, which is prepared from the following raw materials: lavender, clove, wormwood, chamomile, bergamot, blue verbena, red valerian, a penetration enhancer and cannabidiol.

[0006] As a further improvement of the present invention, it is prepared from the following raw materials by weight: 5-7 parts of lavender, 3-5 parts of cloves, 1-2 parts of mugwort, 4-6 parts of chamomile, 5-7 parts of bergamot, 3-5 parts of blue verbena, 2-4 parts of red valerian, 0.01-0.02 parts of penetration enhancer and 0.05-0.1 parts of cannabidiol.

[0007] As a further improvement of the present invention, the penetration enhancer is selected from at least one of laurocapram, eucalyptus oil and peppermint oil.

[0008] As a further improvement of the present invention, the penetration enhancers are laurocapram and eucalyptus oil.

[0009] As a further improvement of the present invention, the mass ratio of laurocapram and eucalyptus oil is 5-7:2.

[0010] The present invention further protects a method for preparing the above-mentioned anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition, comprising the following steps: S1. The lavender, cloves, wormwood, chamomile, bergamot, blue verbena, red valerian grass were crushed, and supercritical fluid extraction was performed to obtain a mixed essential oil, and the solid residue was retained; S2. adding the solid residue in step S1 to an acetone / ethyl acetate solution, heating to reflux for extraction, filtering, and removing the solvent from the filtrate under reduced pressure to obtain an extract; S3. The mixed essential oil obtained in step S1 and the extract obtained in step S2 are mixed uniformly, added to a molecular distiller, heated to a first temperature, removing the first distillate, heated to a second temperature, and collecting a second distillate; S4. The second distillate obtained in step S3, the penetration enhancer and cannabidiol are mixed evenly to prepare a composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep.

[0011] As a further improvement of the present invention, the conditions of the supercritical fluid extraction in step S1 are: temperature 45-55°C, pressure 200-300 Bar, extraction time 60-120 min, and CO2 flow rate 10-20 L / h.

[0012] As a further improvement of the present invention, the volume ratio of acetone to ethyl acetate in the acetone / ethyl acetate solution in step S2 is 1:0.5-1.5.

[0013] As a further improvement of the present invention, the time of heating reflux extraction in step S2 is 30-60 minutes.

[0014] As a further improvement of the present invention, in step S3, the first temperature is 100-120°C, and the second temperature is 150-180°C.

[0015] The present invention has the following beneficial effects: The present invention selects a Chinese medicine composition rich in essential oils with good sleep-promoting and anti-depressant effects, and obtains a mixed essential oil with a very high essential oil concentration through supercritical fluid extraction by a green, safe and efficient method. The mixed essential oil has the effects of relieving stress, calming the nervous system, relieving headaches, soothing emotions, relaxing muscles, promoting sleep, relieving anxiety and depression, soothing anger and frustration, relieving tension, and invigorating the spirit.

[0016] The present invention further extracts the solid residue with an organic solvent to extract most of the oil-soluble active components therein, including flavonoids, saponins, etc., thereby greatly improving the extraction rate of the active components.

[0017] The present invention mixes the mixed essential oil with the extract and extracts it through molecular distillation, thereby intercepting the highly active components, greatly improving the sleep-promoting and anti-depressant effects. After being mixed with a permeation enhancer and cannabidiol, the transdermal absorption efficiency of the composition is greatly improved. Cannabidiol can block the rapid eye movement sleep alternation disorder caused by anxiety, and can also improve various behaviors related to complex sleep and the rapid eye movement sleep alternation disorder of Parkinson's patients. It has the effect of protecting the central nervous system and relieving pain, etc. It can also indirectly help patients relieve tense nerves, effectively help sleep time, and improve the effect of sleep quality.

[0018] The anti-depressant, tranquilizing and sleep-inducing composite cannabidiol essential oil composition prepared by the present invention is safe to use, is extracted from flowers and plants, and has good effects on soothing human emotions, relieving stress and improving insomnia, and has good effects on depression and insomnia. DETAILED DESCRIPTION

[0019] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention. Example

[0020] Raw material composition (by weight): 5 parts of lavender, 3 parts of cloves, 1 part of wormwood, 4 parts of chamomile, 5 parts of bergamot, 3 parts of blue verbena, 2 parts of red valerian, 0.01 parts of penetration enhancer and 0.05 parts of cannabidiol. The penetration enhancer is laurocapron and eucalyptus oil, with a mass ratio of 5:2.

[0021] The preparation method comprises the following steps: S1. The lavender, cloves, wormwood, chamomile, bergamot, blue verbena, red valerian grass were crushed, and supercritical fluid extraction was performed to obtain a mixed essential oil, and the solid residue was retained; The conditions of the supercritical fluid extraction are: temperature 45°C, pressure 200Bar, extraction time 60min, CO2 flow rate 10L / h; S2. 10 parts by weight of the solid residue in step S1 was added to 100 parts by weight of an acetone / ethyl acetate solution (the volume ratio of acetone to ethyl acetate was 1:0.5), heated to reflux for extraction for 30 min, filtered, and the filtrate was decompressed to remove the solvent to obtain an extract; S3. The mixed essential oil obtained in step S1 and the extract obtained in step S2 are mixed uniformly, added to a molecular distiller, heated to 100° C., the first distillate is removed, heated to 150° C., and the second distillate is collected; S4. The second distillate obtained in step S3, the penetration enhancer and cannabidiol are stirred and mixed for 20 minutes to prepare a composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep. Example

[0022] Raw material composition (by weight): 7 parts of lavender, 5 parts of cloves, 2 parts of wormwood, 6 parts of chamomile, 7 parts of bergamot, 5 parts of blue verbena, 4 parts of red valerian, 0.02 parts of penetration enhancer and 0.1 parts of cannabidiol. The penetration enhancer is laurocapone and eucalyptus oil, with a mass ratio of 7:2.

[0023] The preparation method comprises the following steps: S1. The lavender, cloves, wormwood, chamomile, bergamot, blue verbena, red valerian grass were crushed, and supercritical fluid extraction was performed to obtain a mixed essential oil, and the solid residue was retained; The conditions of the supercritical fluid extraction are: temperature 55°C, pressure 300Bar, extraction time 120min, CO2 flow rate 20L / h; S2. 10 parts by weight of the solid residue in step S1 was added to 100 parts by weight of an acetone / ethyl acetate solution (the volume ratio of acetone to ethyl acetate was 1:1.5), heated to reflux for extraction for 30-60 min, filtered, and the filtrate was decompressed to remove the solvent to obtain an extract; S3. The mixed essential oil obtained in step S1 and the extract obtained in step S2 are mixed uniformly, added to a molecular distiller, heated to 120° C., the first distillate is removed, heated to 180° C., and the second distillate is collected; S4. The second distillate obtained in step S3, the penetration enhancer and cannabidiol are stirred and mixed for 20 minutes to prepare a composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep. Example

[0024] Raw material composition (by weight): 6 parts of lavender, 4 parts of cloves, 1.5 parts of wormwood, 5 parts of chamomile, 6 parts of bergamot, 4 parts of blue verbena, 3 parts of red valerian, 0.015 parts of penetration enhancer and 0.07 parts of cannabidiol. The penetration enhancer is laurocapone and eucalyptus oil, with a mass ratio of 6:2.

[0025] The preparation method comprises the following steps: S1. The lavender, cloves, wormwood, chamomile, bergamot, blue verbena, red valerian grass were crushed, and supercritical fluid extraction was performed to obtain a mixed essential oil, and the solid residue was retained; The conditions of the supercritical fluid extraction are: temperature 50°C, pressure 250Bar, extraction time 90min, CO2 flow rate 15L / h; S2. 10 parts by weight of the solid residue in step S1 was added to 100 parts by weight of an acetone / ethyl acetate solution (the volume ratio of acetone to ethyl acetate was 1:1), heated to reflux for extraction for 45 min, filtered, and the filtrate was decompressed to remove the solvent to obtain an extract; S3. The mixed essential oil obtained in step S1 and the extract obtained in step S2 are mixed uniformly, added to a molecular distiller, heated to 110° C., the first distillate is removed, heated to 165° C., and the second distillate is collected; S4. The second distillate obtained in step S3, the penetration enhancer and cannabidiol are stirred and mixed for 20 minutes to prepare a composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep. Example

[0026] Compared with Example 3, the difference is that the penetration enhancer is laurocapram. Example

[0027] Compared with Example 3, the difference is that the penetration enhancer is eucalyptus oil.

[0028] Comparative Example 1 Compared with Example 3, the difference is that no penetration enhancer is added.

[0029] Comparative Example 2 Compared with Example 3, the difference is that cannabidiol is not added.

[0030] Test Example 1 90 healthy Wistar rats, weighing 180-220g, were randomly divided into a normal group, a model group, an embodiment 1-5 group, and a comparative example 1-2 group, with 10 rats in each group. All rats in each group were free to drink water, eat, and maintain natural light under the same conditions. Rats usually sleep during the day and are active at night. Except for the normal group, rats in other groups were intraperitoneally injected with PCPA 300mg / kg. After 28-30h, this circadian rhythm disappeared, and they were also active during the day. The total amount of sleep was small and almost reached complete insomnia. Rats in the embodiment 1-5 and comparative example 1-2 groups were smeared with 0.1mL of the corresponding anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition on the nose every day, and the normal group and the model group were smeared with an equal amount of physiological saline. The drug was administered continuously for 7 days. The rats were decapitated, the skulls were removed, and the brain tissues were collected to detect the contents of 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA), norepinephrine (NA), and dopamine (DA). The anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition prepared in Examples 1-3 of the present invention can increase the content of 5-HT and 5-HIAA in rat brain tissue and reduce the content of NA and DA.

[0031] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep, characterized in that: It is prepared from the following ingredients: lavender, clove, wormwood, chamomile, bergamot, blue verbena, red valerian, penetration enhancers and cannabidiol.

2. The anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition according to claim 1, characterized in that: The invention is prepared from the following raw materials in parts by weight: 5-7 parts of lavender, 3-5 parts of cloves, 1-2 parts of mugwort, 4-6 parts of chamomile, 5-7 parts of bergamot, 3-5 parts of blue verbena, 2-4 parts of red valerian, 0.01-0.02 parts of a penetration enhancer and 0.05-0.1 parts of cannabidiol.

3. The anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition according to claim 1, characterized in that: The penetration enhancer is selected from at least one of laurocapram, eucalyptus oil and peppermint oil.

4. The anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition according to claim 3, characterized in that: The penetration enhancers are laurocapram and eucalyptus oil.

5. The anti-depressant, tranquilizing, and sleep-inducing composite cannabidiol essential oil composition according to claim 4, characterized in that: The mass ratio of laurocapram and eucalyptus oil is 5-7:

2.

6. A method for preparing the anti-depressant, tranquilizing and sleep-inducing composite cannabidiol essential oil composition as claimed in any one of claims 1 to 5, characterized in that: The following steps are involved: S1. The lavender, cloves, wormwood, chamomile, bergamot, blue verbena, red valerian grass were crushed, and supercritical fluid extraction was performed to obtain a mixed essential oil, and the solid residue was retained; S2. adding the solid residue in step S1 to an acetone / ethyl acetate solution, heating to reflux for extraction, filtering, and removing the solvent from the filtrate under reduced pressure to obtain an extract; S3. The mixed essential oil obtained in step S1 and the extract obtained in step S2 are mixed uniformly, added to a molecular distiller, heated to a first temperature, removing the first distillate, heated to a second temperature, and collecting a second distillate; S4. The second distillate obtained in step S3, the penetration enhancer and cannabidiol are mixed evenly to prepare a composite cannabidiol essential oil composition for anti-depression, tranquilizing and inducing sleep.

7. The preparation method according to claim 6, characterized in that: The conditions of the supercritical fluid extraction in step S1 are: temperature 45-55°C, pressure 200-300 Bar, extraction time 60-120 min, and CO2 flow rate 10-20 L / h.

8. The preparation method according to claim 6, characterized in that: The volume ratio of acetone to ethyl acetate in the acetone / ethyl acetate solution in step S2 is 1:0.5-1.

5.

9. The preparation method according to claim 6, characterized in that: The time of heating reflux extraction in step S2 is 30-60 minutes.

10. The preparation method according to claim 6, characterized in that: In step S3, the first temperature is 100-120°C, and the second temperature is 150-180°C.