A large-caliber spring chromatographic column structure

Abstract

The present application discloses a large-caliber spring chromatographic column structure, which relates to the technical field of chromatographic column determination, and comprises: a column body, wherein a lower end cover and a threaded flange are respectively arranged at the upper and lower ends, the lower end cover is fixed with a plurality of support rods by nuts, the threaded flange is fixedly connected to the support rods by nuts, a feed interface is arranged on the threaded flange, and a discharge interface is arranged on the lower end cover; an adjustment component is slidably arranged in the column body, a spring component and a piston component are sequentially connected at the lower end of the adjustment component; a packed bed layer is arranged inside the column body, and the packed bed layer is located between the lower end cover and the piston assembly, so as to provide a technical solution that is easy to adapt to pressure specifications and improve applicability.

Description

Technical Field

[0001] The present application relates to the technical field of chromatographic column determination, and in particular to a large-caliber spring chromatographic column structure. Background Art

[0002] Spring columns are a type of equipment used for chromatographic analysis and are suitable for high-pressure preparative chromatography. The design of spring columns allows for automatic column loading, reduces column bed rebound during column loading, maintains column efficiency, and extends the service life of the column. In this type of column, the role of the spring is to provide the necessary preload to ensure the stability and reproducibility of the column under high pressure.

[0003] However, under different demand conditions of spring chromatography columns, spring kits with different pressure ranges are selected to ensure that the spring force exceeds the working pressure of the column and maintain the axial compression force. In addition, for different column lengths, the sizes of the filling column bed and the spring kit interfere with each other and are difficult to adjust.

[0004] Therefore, it is necessary to provide a large-caliber spring chromatographic column structure to solve the problems raised in the above background technology. Summary of the invention

[0005] To achieve the above purpose, the present application provides the following technical solution: a large-caliber spring chromatographic column structure, comprising:

[0006] The column body is provided with a lower end cover and a threaded flange at the upper and lower ends respectively, the lower end cover is fixed with a plurality of support rods by nuts, the threaded flange is fixedly connected with the support rods by nuts, and a feed interface is provided on the threaded flange, and a discharge interface is provided on the lower end cover;

[0007] An adjusting component is slidably disposed in the column, and a spring component and a piston component are sequentially connected to the lower end of the adjusting component;

[0008] A packed bed layer is arranged inside the column, and the packed bed layer is located between the lower end cover and the piston assembly;

[0009] The adjusting assembly includes a threaded shaft and a feed pipe, wherein the threaded shaft is fixed by cooperating with the threaded flange, one end of the feed pipe is connected to the feed interface, and the other end passes through the spring assembly and the piston assembly and is connected to the packed bed.

[0010] Preferably, the piston assembly comprises a piston body and a piston filter, a sealing ring is sleeved on the circumference of the piston body, and the piston body is in close contact with the inner wall of the cylinder.

[0011] Preferably, the adjustment assembly also includes an adapter seat, an adjustment rod, an auxiliary spring and an adjustment nut. The upper end of the adapter seat is fixedly connected to the threaded shaft, and the adjustment rod is arranged in multiples. The upper end of the adjustment rod passes through the adapter seat and is fixed by the adjustment nut, and the auxiliary spring is sleeved on the adjustment rod.

[0012] Preferably, the lower end of the adjusting rod extends into the interior of the spring assembly.

[0013] Preferably, the spring assembly comprises an end plate and spring leaves, the spring leaves are stacked and arranged to form a spring group, and the end plate is located at the upper end of the spring group, and the lower end of the spring group is connected to the piston body.

[0014] Preferably, a plurality of through holes are formed on the surface of the spring sheet, and the through holes allow the adjusting rod to pass through.

[0015] Preferably, the spring sheets can be deflected and adjusted relative to each other, and there are multiple fixing positions for the lower end of the adjusting rod, one is to fix it directly on the end plate, and the other is to adjust the deflection of the spring sheet so that the adjusting rod passes through the end plate and part of the spring sheet abuts against the surface of the remaining spring sheets.

[0016] Compared with the prior art, the present application provides a large-caliber spring chromatographic column structure, which has the following beneficial effects:

[0017] In the present application, the position of the lower end of the adjusting rod is fixed to the adjustable spring sheet, which can apply the upper limit of the preload to the packed bed layer. On the other hand, the adjusting rod passes through part of the spring sheet to control the overall length of the adjusting assembly and the spring assembly, adapting to different columns and realizing adaptation of various specifications;

[0018] It can automatically replenish the gaps formed by the sinking of the column bed during the use of the packed bed, extend the service life, and improve the overall performance. The spring contraction principle is used to maintain the axial pressure of the packed bed. The pressure inside the chromatographic column is stably maintained. As the packed bed gradually contracts, the spring sheet stretches to make up for the dead volume and prevent the generation of gaps. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Other features, objects and advantages of the present application will become more apparent by reading the detailed description of non-limiting embodiments with reference to the following drawings:

[0020] Figure 1 It is a schematic diagram of the overall structure of a large-diameter spring chromatographic column structure;

[0021] Figure 2 It is a schematic diagram of the internal components structure of a large-caliber spring chromatographic column structure;

[0022] Figure 3 for Figure 2 A schematic diagram of the structure enlargement in the middle;

[0023] Figure 4 It is a schematic diagram of the structure of an adjustment component of a large-caliber spring chromatographic column structure;

[0024] Figure 5 A schematic diagram of the spring assembly structure of a large-caliber spring chromatographic column structure;

[0025] Figure 6 A schematic diagram of a spring sheet structure of a large-caliber spring chromatographic column structure;

[0026] In the figure: 1. column; 2. lower end cover; 21. discharge interface; 3. threaded flange; 4. feed interface; 5. support rod; 6. adjustment assembly; 61. threaded shaft; 62. feed pipe; 63. adapter; 64. adjustment rod; 65. auxiliary spring; 66. adjustment nut; 7. spring assembly; 71. end plate; 72. spring sheet; 721. through hole; 8. piston assembly; 81. piston body; 82. piston filter; 9. packed bed. DETAILED DESCRIPTION

[0027] See also Figure 1-Figure 6 In an embodiment of the present application, a large-caliber spring chromatographic column structure comprises:

[0028] The column 1 is provided with a lower end cover 2 and a threaded flange 3 at the upper and lower ends respectively, the lower end cover 2 is fixed with a plurality of support rods 5 by nuts, the threaded flange 3 is fixedly connected with the support rods 5 by nuts, and a feed interface 4 is provided on the threaded flange 3, and a discharge interface 21 is provided on the lower end cover 2;

[0029] An adjusting component 6 is slidably disposed in the column 1, and a spring component 7 and a piston component 8 are sequentially connected to the lower end of the adjusting component 6;

[0030] The packed bed layer 9 is arranged inside the column 1 , and the packed bed layer 9 is located between the lower end cover 2 and the piston assembly 8 .

[0031] As a preferred embodiment, the adjusting assembly 6 includes a threaded shaft 61 and a feed pipe 62, the threaded shaft 61 is fixed by cooperating with the threaded flange 3, one end of the feed pipe 62 is connected to the feed interface 4, and the other end passes through the spring assembly 7 and the piston assembly 8 and is connected to the packed bed 9.

[0032] It should be explained that, during use, the adjustment assembly 6, the spring assembly 7 and the piston assembly 8 are first assembled and uniformly inserted into the column 1 for adjustment, and then pressure is applied by squeezing the threaded shaft 61 to promote the piston assembly 8 to squeeze the packed bed 9, and then the threaded flange 3, the threaded shaft 61 and the support rod 5 are fixed together to ensure that a pre-tightening force is applied to the packed bed 9, and then the material is input through the feed interface 4 for processing;

[0033] The adjusting assembly 6 and the spring assembly 7 are adapted to the length of the column 1 and the pressure requirement of the packed bed 9 to perform pressure and length adjustment.

[0034] As a preferred embodiment, the piston assembly 8 includes a piston body 81 and a piston filter 82 . A sealing ring is sleeved on the circumference of the piston body 81 , and the piston body 81 is in close contact with the inner wall of the cylinder 1 .

[0035] As a preferred embodiment, the adjustment assembly 6 also includes an adapter seat 63, an adjustment rod 64, an auxiliary spring 65 and an adjustment nut 66. The upper end of the adapter seat 63 is fixedly connected to the threaded shaft 61, and the adjustment rod 64 is arranged in multiples. The upper end of the adjustment rod 64 passes through the adapter seat 63 and is fixed by the adjustment nut 66, and the auxiliary spring 65 is sleeved on the adjustment rod 64.

[0036] It should be explained that, by controlling the length of the adjusting rod 64 passing upward through the adapter 63 , the overall length of the adjusting assembly 6 can be controlled, thereby improving the adaptability to different length requirements of the column 1 .

[0037] As a preferred embodiment, the lower end of the adjusting rod 64 extends into the interior of the spring assembly 7 .

[0038] As a preferred embodiment, the spring assembly 7 includes an end plate 71 and a spring sheet 72 , the spring sheets 72 are stacked and arranged to form a spring group, and the end plate 71 is located at the upper end of the spring group, and the lower end of the spring group is connected to the piston body 81 .

[0039] As a preferred embodiment, a plurality of through holes 721 are formed on the surface of the spring sheet 72 , and the through holes 721 allow the adjusting rod 64 to pass through.

[0040] As a preferred embodiment, the spring sheets 72 can be deflected and adjusted relative to each other, and there are multiple fixing positions for the lower end of the adjusting rod 64. One is to fix it directly on the end plate 71, and the other is to adjust the deflection of the spring sheet 72 so that the adjusting rod 64 passes through the end plate 71 and part of the spring sheet 72 and abuts against the surface of the remaining spring sheets 72.

[0041] It should be explained that the fixed position of the lower end of the adjusting rod 64 can adjust the spring sheet 72, so that the upper limit of the preload can be applied to the packed bed 9. On the other hand, the adjusting rod 64 passes through part of the spring sheet 72 to control the overall length of the adjusting assembly 6 and the spring assembly 7, adapting to different columns 1 and realizing adaptation of various specifications.

[0042] The automatic filling of the gaps formed by the sinking of the column bed during the use of the packed bed layer 9 can extend the service life and improve the overall performance. The axial pressurization of the packed bed layer 9 is maintained by utilizing the contraction principle of the spring. The pressure is stably maintained inside the chromatographic column. As the packed bed layer 9 gradually contracts, the spring sheet 72 stretches to make up for the dead volume and prevent the generation of gaps.

[0043] In the specific implementation, the adjustment component 6, the spring component 7 and the piston component 8 are assembled and uniformly inserted into the column 1 for adjustment, and then pressure is applied by squeezing the threaded shaft 61 to promote the piston component 8 to squeeze the packed bed layer 9, and then the threaded flange 3, the threaded shaft 61 and the support rod 5 are fixed together to ensure that a pre-tightening force is applied to the packed bed layer 9, and then the material is input through the feed interface 4 for processing;

[0044] The fixed position of the lower end of the adjusting rod 64 can adjust the spring sheet 72 to apply the upper limit of the preload to the packed bed 9. On the other hand, the adjusting rod 64 passes through part of the spring sheet 72 to control the overall length of the adjusting component 6 and the spring component 7, adapting to different columns 1 and achieving adaptation of various specifications.

[0045] What has been described above is only a preferred specific implementation manner of the present application, but the protection scope of the present application is not limited thereto. Any technician familiar with the technical field can make equivalent substitutions or changes within the technical scope disclosed in the present application according to the technical solution and application concept of the present application, which should be covered by the protection scope of the present application.

Claims

1. A large-caliber spring chromatographic column structure, characterized in that: include: The column (1) is provided with a lower end cover (2) and a threaded flange (3) at the upper and lower ends respectively; the lower end cover (2) is fixed with a plurality of support rods (5) via nuts; the threaded flange (3) is fixedly connected to the support rods (5) via nuts; a feed interface (4) is provided on the threaded flange (3); and a discharge interface (21) is provided on the lower end cover (2); An adjusting component (6) is slidably disposed in the column (1), and a spring component (7) and a piston component (8) are sequentially connected to the lower end of the adjusting component (6); A packed bed layer (9) is arranged inside the column (1), and the packed bed layer (9) is located between the lower end cover (2) and the piston assembly (8); The adjusting assembly (6) comprises a threaded shaft (61), a feed pipe (62), an adapter seat (63), an adjusting rod (64), an auxiliary spring (65) and an adjusting nut (66); the threaded shaft (61) is fixed by cooperating with the threaded flange (3); one end of the feed pipe (62) is connected to the feed interface (4); the other end passes through the spring assembly (7) and the piston assembly (8) and is connected to the packed bed layer (9); the upper end of the adapter seat (63) is fixedly connected to the threaded shaft (61); a plurality of adjusting rods (64) are arranged; the upper ends of the adjusting rods (64) pass through the adapter seat (63) and are fixed by the adjusting nut (66); and the auxiliary spring (65) is sleeved on the adjusting rod (64); The spring assembly (7) comprises an end plate (71) and a spring sheet (72), the spring sheets (72) are stacked and arranged to form a spring group, and the end plate (71) is located at the upper end of the spring group, and the lower end of the spring group is connected to the piston body (81); The spring sheets (72) can be deflected and adjusted relative to each other. There are multiple fixing positions for the lower end of the adjusting rod (64), one of which is to be directly fixed on the end plate (71), and the other is to adjust the deflection of the spring sheet (72) so that the adjusting rod (64) penetrates the end plate (71) and part of the spring sheet (72) abuts against the surface of the remaining spring sheet (72).

2. A large-caliber spring chromatographic column structure according to claim 1, characterized in that: The piston assembly (8) comprises a piston body (81) and a piston filter (82). A sealing ring is sleeved around the circumference of the piston body (81), and the piston body (81) is in close contact with the inner wall of the column (1).

3. A large-caliber spring chromatographic column structure according to claim 1, characterized in that: The lower end of the adjustment rod (64) extends into the interior of the spring assembly (7).

4. A large-caliber spring chromatographic column structure according to claim 1, characterized in that: A plurality of through holes (721) are formed on the surface of the spring sheet (72), and the through holes (721) allow the adjustment rod (64) to pass through.

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