Composite seedling raising method of savatier monochasma herb-host

Abstract

The invention discloses a monochasma savatieri-host composite seedling raising method. The method comprises the following steps: selecting a non-woven fabric bag or a plug as a culture container; field soil, nutrient soil, fine sand and vermiculite are mixed to serve as a substrate; selecting plump monochasma savatieri seeds, soaking the monochasma savatieri seeds with gibberellin, cleaning the monochasma savatieri seeds with The substrate is wetted thoroughly before sowing; mixing savatier monochasma herb seeds with fine sand, and then sowing the mixture on the soil surface of the substrate to wet the soil surface; wheat seeds or digitaria sanguinalis seeds are sown in the matrix where the savatier monochasma herb is sown; and illumination and nutrient management: when the savatier monochasma herb grows to a cotyledon stage and a true leaf stage, culturing under a high light intensity condition, and irrigating a seaweed nutrient solution and rooting powder. According to the monochasma savatieri-host composite seedling raising method, a composite seedling raising system is established through monochasma savatieri and the gramineous plant wheat or digitaria sanguinalis, the seedling breeding speed of monochasma savatieri is increased, and artificial large-scale cultivation is promoted.

Description

Technical Field

[0001] The invention belongs to the technical field of medicinal plant cultivation, and particularly relates to a composite seedling cultivation method of Pterocarpus shavarens-host. Background Art

[0002] Monochasma savatieri Franch.ex Maxim is the dried whole herb of Monochasma savatieri, a plant of the genus Monochasma in the family Scrophulariaceae. It is densely covered with cotton wool and appears grayish white, so it is also known as white-headed gentian. The plant of Monochasma savatieri can be up to 20-30 cm tall, and its stems will wither every other year. The underground rhizome is thick and short, woody, and the fibrous roots are relatively developed and dense. The stem leaves are alternately opposite and dense, and are oblong lanceolate to linear lanceolate.

[0003] Sha's Deer Antler Grass is distributed in Zhejiang, Fujian, Jiangxi, Hubei and Hunan provinces in my country. It mostly grows in weeds in sunny places on hillside woodlands and is also found under Masson pine forests. It is a perennial semi-parasitic herb and its whole plant can be used as medicine. According to the records of "Jiangxi Herbal Medicine" and "Hangzhou Medicinal Plant Records", the whole plant of Deer Antler Grass has the effects of clearing heat and detoxifying, cooling blood and stopping bleeding. Sha's Deer Antler Grass has a wide range of clinical applications, such as for the treatment of colds, wind-heat cough and asthma, toothache, rheumatic bone pain, thrush in children and traumatic bleeding.

[0004] In recent years, the demand for Deer Antler Grass in the medical market has been increasing. Due to environmental damage and over-harvesting, the number of Deer Antler Grass growing in the wild is decreasing. However, in the process of artificial cultivation of Deer Antler Grass, there are problems such as low seedling survival rate and long growth cycle.

[0005] The prior art discloses a method for planting S. saburoi in potted plants, in which S. saburoi parasitizes crabgrass to improve the seed germination rate. However, this method requires that the host grows stably and well before subsequent sowing of S. saburoi seeds, that is, the host needs to be cultivated before sowing. If the host withers, it cannot reproduce well into seedlings. Summary of the invention

[0006] In order to address the deficiencies of the prior art, the present invention provides a composite seedling cultivation method of Sha'er's Deer Antler Grass-host, by establishing a composite seedling cultivation system of Sha'er's Deer Antler Grass and Gramineae plants wheat or crabgrass, thereby improving the seedling breeding speed of Sha'er's Deer Antler Grass and promoting artificial large-scale cultivation of Sha'er's Deer Antler Grass.

[0007] The technical effects to be achieved by the present invention are achieved through the following technical aspects:

[0008] A composite seedling raising method of Pterocarpus shashii-host comprises the following steps:

[0009] S1. Select a culture container: Select a non-woven bag or a plug tray as a culture container;

[0010] S2. Substrate treatment: Take garden soil, nutrient soil, fine sand and vermiculite, mix them in a volume ratio of 1:1.5 to 2:1:1 as the substrate, and put them into the culture container;

[0011] S3. Treatment of P. saburoides seeds: Select seeds of P. saburoides with full grains, soak them in gibberellin for 24 to 30 hours, wash them with distilled water for 3 to 4 times, and sow them immediately after natural air drying;

[0012] S4. Sowing: Before sowing, first water the substrate to moisten thoroughly; the S3 treated velvet grass seeds and fine sand in a volume ratio of 1: 8 to 20 ratio after mixing, and then evenly sowed on the soil surface of the substrate, with a micro-sprinkler to moisten the soil surface;

[0013] S5. Planting host: Sow wheat seeds or crabgrass seeds in the substrate sown with S. serrata, or sow wheat seeds and crabgrass seeds at the same time;

[0014] S6. Light and nutrient management: When the safflower grass grows to the cotyledon stage and true leaf stage, it needs to be cultivated under high light intensity conditions;

[0015] For the Sha's Deer Antler Grass in the cotyledon stage and true leaf stage, seaweed nutrient solution and rooting powder should be applied.

[0016] In some embodiments, the sowing step in S4 further includes the following steps:

[0017] S41. Water management: After sowing the velvet antler grass seeds on the soil surface of the substrate, water 1 to 2 times every 1 to 2 days using a micro sprinkler.

[0018] In some embodiments, in each non-woven bag or each cell, the sowing ratio of the Pterocarpus sabdariffa seeds to the wheat seeds or the crabgrass seeds is 8-10:1.

[0019] In some embodiments, in S5, the time of planting host wheat or crabgrass is at least one month after sowing P. saburoi.

[0020] In some embodiments, in S2 and S4, the fine sand is river sand, and the fine sand is sieved through a 20-40 mesh sieve.

[0021] In some embodiments, in S2, 3-5 days before formal sowing, the carbendazim solution is diluted into water at a volume ratio of 1:2000-2500, and the substrate is irrigated with the diluted carbendazim aqueous solution to thoroughly moisten it.

[0022] In some embodiments, in S3, the seeds of P. saburoi are soaked in gibberellin, and the concentration of the gibberellin solution is 800-1000 mg / L.

[0023] In some embodiments, in the light management of S6, the Salix babylonica in the cotyledon stage and the true leaf stage needs to be cultured under high light intensity conditions, and the high light intensity conditions are 5000-10000 Lux; if the natural light is not enough, supplementary light treatment is required.

[0024] In some embodiments, in the nutrient management of S6, the seaweed nutrient solution is a 200-fold dilution of concentrated seaweed nutrient solution, and the irrigation frequency is once a day; the concentration of the rooting powder is 15-18 mg / L, and the irrigation frequency is once a week.

[0025] In some embodiments, as the host of P. shavarens, the wheat is an annual or biennial herbaceous plant of the genus Triticum in the Poaceae family; the crabgrass is an annual herbaceous plant of the genus Digitaria in the Poaceae family;

[0026] Planting the host plant one month after sowing the safflower can:

[0027] Sow wheat seeds and crabgrass seeds at the same time;

[0028] Or you can sow wheat seeds first, and then sow crabgrass seeds 10-20 days later;

[0029] Or you can sow crabgrass seeds first, and then sow wheat seeds 10-20 days later;

[0030] Or just broadcast wheat seeds or crabgrass seeds.

[0031] In summary, the present invention has at least the following benefits:

[0032] 1. The composite seedling raising method of Shashi's velvet antler grass-host provided by the present invention establishes a composite seedling raising system with Shashi's velvet antler grass and Gramineae plants wheat or crabgrass. The time for planting the host wheat or crabgrass needs to be at least one month after the sowing of Shashi's velvet antler grass, ensuring that the composite seedling raising is carried out after the parasitic relationship between Shashi's velvet antler grass and wheat or crabgrass is established. On the one hand, the composite seedling raising method improves the seed germination rate of Shashi's velvet antler grass, and on the other hand, it improves the artificial cultivation success rate and seedling breeding speed of Shashi's velvet antler grass, so as to ensure the sufficient supply of Shashi's velvet antler grass materials.

[0033] 2. The composite seedling raising method of the host of the Sha'er's antler grass provided by the present invention successfully realizes the artificial cultivation of Sha'er's antler grass by planting in a non-woven bag or a plug tray. The cultivation method is easy to operate, the planted Sha'er's antler grass has good growth conditions, can promote the biomass accumulation of Sha'er's antler grass, and is conducive to large-scale artificial planting. BRIEF DESCRIPTION OF THE DRAWINGS

[0034] Figure 1 It is a composite seedling formed by the sabdariffa of the present invention and a herbaceous host.

[0035] Figure 2 The composite seedlings are the sabdariffa of the present invention and the herbaceous host plant transplanted into a greenhouse.

[0036] Figure 3 The invention discloses a composite seedling of the sachsengrass and a herbaceous host planted in a wild nurturing mountain. DETAILED DESCRIPTION

[0037] For ease of understanding of the present invention, the present invention will be described more fully below in conjunction with the accompanying drawings and specific embodiments. Preferred embodiments of the present invention are provided in the embodiments. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present invention more thoroughly understood.

[0038] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present invention. The terms used in the specification of the present invention herein are only for the purpose of describing specific embodiments and are not intended to limit the present invention.

[0039] References in the specification to "one embodiment", "embodiment", "exemplary embodiment", etc. mean the embodiment being described, but not every embodiment may include a particular feature, structure, or characteristic. Specific features, structures, or characteristics are included. Moreover, such phrases do not necessarily refer to the same embodiment. In addition, when specific features, structures, or characteristics are described in conjunction with certain embodiments, whether or not explicitly described, it is within the knowledge of those skilled in the art to consider the effects of applying the features, structures, or characteristics to other embodiments.

[0040] The values ​​expressed as ranges should be interpreted in a flexible manner, including not only the values ​​explicitly listed as the limits of the range, but also all individual values ​​or sub-ranges included in the range, as if each value and sub-range were clearly stated. For example, a (mass) concentration range of "about 0.1% to about 5%" should be interpreted as including not only the explicitly listed values ​​of about 0.1% to about 5% by weight, but also the individual (mass) concentrations (e.g., 1%, 2%, 3% and 4%) and sub-ranges (e.g., 0.1% to 0.5%, 1.1% to 2.2% and 3.3% to 4.4%) within the specified range.

[0041] As described herein, unless stated otherwise, the terms "a" and "an" are used to include one or more than one, and the term "or" is used to refer to a non-exclusive "or". In addition, when a term or terms used herein are not otherwise qualified, they should be understood to be used for descriptive purposes only and not for limiting purposes. In addition, all publications, patents, and patent documents mentioned in the specification are incorporated herein by reference in their entirety, as if individually incorporated by introduction. If there is an inconsistency in usage between this document and those documents incorporated by reference, the usage in the cited references should be considered to supplement this document. For irreconcilable inconsistencies, the usage in this document shall prevail.

[0042] A composite seedling raising method of Pterocarpus shashii-host comprises the following steps:

[0043] S1. Select a culture container: Select a non-woven bag or a plug tray as a culture container;

[0044] S2. Substrate treatment: Take garden soil, nutrient soil, fine sand and vermiculite, mix them in a volume ratio of 1:1.5 to 2:1:1 as the substrate, and put them into the culture container;

[0045] S3. Treatment of P. saburoides seeds: Select seeds of P. saburoides with full grains, soak them in gibberellin for 24 to 30 hours, wash them with distilled water for 3 to 4 times, and sow them immediately after natural air drying;

[0046] S4. Sowing: Before sowing, first water the substrate to make it wet; mix the velvet antler grass seeds treated in S3 with fine sand in a volume ratio of 1:8 to 20, and then evenly spread them on the soil surface of the substrate, and moisten the soil surface with a micro-sprinkler;

[0047] S5. Planting host: Sow wheat seeds or crabgrass seeds in the substrate sown with S. serrata, or sow wheat seeds and crabgrass seeds at the same time;

[0048] S6. Light and nutrient management: When the safflower grass grows to the cotyledon stage and true leaf stage, it needs to be cultivated under high light intensity conditions;

[0049] For the Sha's Deer Antler Grass in the cotyledon stage and true leaf stage, seaweed nutrient solution and rooting powder should be applied.

[0050] In the composite seedling raising method of the sabendula velvet-host plant, the sabendula velvet is artificially planted in a planting bag, specifically, the sabendula velvet is planted in a non-woven bag or a plug tray, the substrate of the non-woven bag or the plug tray is made of garden soil, nutrient soil, fine sand and vermiculite, and is irrigated with a carbendazim solution.

[0051] The inventors found that the shamrock antler grass has a companion relationship with wheat or crabgrass, that is, the shamrock antler grass parasitizes on wheat or crabgrass, and its growth trend is good. The inventors also found that if the host wheat or crabgrass grows to a certain height, it will hinder the germination of shamrock antler grass seeds and the growth of seedlings to a certain extent.

[0052] Therefore, during the planting process, before sowing host wheat or crabgrass, it is necessary to plant Deer Antler Grass first. Only after the Deer Antler Grass has grown stably and well, can host wheat or crabgrass be sown, thereby establishing a stable parasitic relationship between Deer Antler Grass and host wheat or crabgrass, so that the seedlings of Deer Antler Grass can grow quickly and stably and the plants can be strong.

[0053] The seeding step in S4 further includes the following steps:

[0054] S41. Water management: After sowing the velvet antler grass seeds on the soil surface of the substrate, water 1 to 2 times every 1 to 2 days using a micro sprinkler.

[0055] In each non-woven bag or each cell, the sowing ratio of the seeds of the sabdariffa to the seeds of wheat or crabgrass is 8 to 10: 1. Through this sowing ratio, the planting density of the parasite sabdariffa and the host wheat or crabgrass is balanced to ensure that the sabdariffa grows rapidly after the seeds germinate.

[0056] Wherein, in step S5, the time for planting host wheat or crabgrass should be at least one month after sowing the safflower.

[0057] After experiments and screening, the inventors found that planting wheat or crabgrass at least one month after sowing the seeds of the Sawyer's antler grass is conducive to the establishment of a parasitic relationship between the Sawyer's antler grass and wheat or crabgrass, as well as the subsequent biomass accumulation of the Sawyer's antler grass.

[0058] Wherein, in steps S2 and S4, the fine sand is river sand, and the fine sand is sieved through a 20-40 mesh sieve.

[0059] Wherein, in step S2, 3-5 days before formal sowing, the carbendazim solution is diluted into water at a volume ratio of 1:2000-2500, and the substrate is irrigated with the diluted carbendazim aqueous solution to wet it thoroughly.

[0060] Wherein, in step S3, the seeds of P. saburoi are soaked in gibberellin, and the concentration of the gibberellin solution is 800-1000 mg / L.

[0061] Among them, in the light management of step S6, the Salix babylonica in the cotyledon stage and true leaf stage needs to be cultivated under high light intensity conditions, and the high light intensity condition is 5000-10000 Lux; if the natural light is not enough, supplementary light treatment is required.

[0062] Under the cultivation conditions of higher light intensity (5000-10000Lux), the growth of P. saburoides in the cotyledon and true leaf stages can be significantly promoted, and the biomass accumulation of wheat or crabgrass can be significantly increased, while also avoiding excessive moisture content in the substrate.

[0063] Among them, in the nutrient management of step S6, the seaweed nutrient solution is a 200-fold dilution of the concentrated seaweed nutrient solution, and the irrigation frequency is once a day; the concentration of the rooting powder is 15-18 mg / L, and the irrigation frequency is once a week.

[0064] As the host of P. shassoni, wheat is an annual or biennial herbaceous plant of the genus Triticum in the Poaceae family; Digitaria is an annual herbaceous plant of the genus Digitaria in the Poaceae family;

[0065] Plant the host one month after sowing the Sha's Deer Antler Grass. You can sow wheat seeds and crabgrass seeds at the same time; or sow wheat seeds first and then sow crabgrass seeds 10-20 days later; or sow crabgrass seeds first and then sow wheat seeds 10-20 days later; or only sow wheat seeds or crabgrass seeds.

[0066] Example 1

[0067] Experimental study on the effects of different substrate ratios and seedling containers on the germination of Pterocarpus sabdariffa

[0068] A method for planting saburo velvet grass by composite seedling cultivation is carried out according to the following steps:

[0069] (1) Substrate ratio and seedling container selection

[0070] Two treatment groups were set up with substrate ratios: G1 [garden soil: nutrient soil: fine sand = 1:1:1 (v / v)] and G2 [mountain soil: nutrient soil: fine sand: vermiculite = 1:2:1:1 (v / v)].

[0071] Three treatment groups were set up in the seedling raising containers: D1 [black seedling raising pot 3 cm × 6 cm (diameter × height)], D2 [small non-woven bag 4 cm × 12 cm (diameter × height)], and D3 [large non-woven bag 6 cm × 12 cm (diameter × height)].

[0072] (2) Sowing time of Pterocarpus serrata and its host Digitaria

[0073] Twenty seeds of Psoralea corylifolia were sown in each treatment group on October 29, 2023, and three seeds of Crabgrass were sown on November 29.

[0074] (3) Cultivation management

[0075] During the germination process of Sha's Deer Antler Grass seeds, water was sprayed to keep them moist. The non-woven bag was placed in a plastic seedling tray (46cm×46cm×10cm, length×width×height) and covered with a film. The film was removed after the seedlings were all grown.

[0076] Seaweed nutrient solution (1:200 dilution): spray evenly in each non-woven bag in equal amounts, and manage routinely. Observe the germination of the seeds of P. serrata every 24 hours and count the number of germinations. The germination of P. serrata seeds is based on the emergence of two cotyledons. The germination test ends when no new seedlings emerge from the test seeds for 3 consecutive days. The results are shown in Table 1.

[0077] Table 1 Germination indexes of Pterocarpus serrata treated with different substrates and seedling containers

[0078]

[0079]

[0080] As can be seen from Table 1, the germination rate, germination potential, germination index and vitality index of the G2D3 treatment group were the highest, significantly higher than those of other treatment groups, followed by the G2D2 treatment group. The germination rate of the G2D3 treatment group was 14.5 percentage points higher than that of G1D2. The lowest values ​​of germination rate, germination potential, germination index and vitality index appeared in the G1D2, G1D1, G1D1 and G1D1 treatment groups, respectively.

[0081] Example 2

[0082] Experimental study on the effects of supplementary light and rooting agent on the growth indexes of Pterocarpus sabdariffa at the cotyledon stage

[0083] A method for planting saburo velvet grass by composite seedling cultivation is carried out according to the following steps:

[0084] (1) Selection of seedling containers and substrate treatment

[0085] A small non-woven bag with a specification of 4cm×12cm (diameter×height) was selected for the composite seedling cultivation of Deer Antler Grass and Digitaria sanguinea. The substrate was selected from garden soil, nutrient soil (Cuiyun Liangtu), fine sand and vermiculite, with the ratio of mountain soil: nutrient soil: fine sand: vermiculite = 1:2:1:1.

[0086] (2) Seed treatment

[0087] The seeds of P. sabdariffa were soaked in 800 mg / L gibberellin solution for 24 hours, washed with distilled water for 3-4 times, and air-dried. The seeds of Crabgrass were air-dried and placed in a cool place for later use.

[0088] (3) Seed planting

[0089] Prioritize planting Sachsengrass seeds, sow 20 seeds in each seedling container, and sow crabgrass seeds one month later, sow 3 seeds in each seedling container.

[0090] (4) Cultivation management

[0091] An LED supplementary light (light intensity of 5000-8000 Lux) was arranged about 40 cm above the seedling container for supplementary lighting, and the supplementary lighting time was 7:30-19:30. At the same time, seaweed concentrated nutrient solution (diluted 200 times) was poured once a day, and 40 mL was poured into each bag of seedling container each time. The seedlings of P. saburoi were treated with rooting powder at a concentration of 15 mg / L, once a week, and the treatment lasted for 5 weeks.

[0092] This method of growing seedlings of Sha'er's Deer Antler Grass and its host Digitaria, through technical treatments such as substrate ratio, seedling container selection, LED light supplementary lighting, and fertilization, has resulted in extremely good indicators such as Sha'er's Deer Antler Grass in terms of seedling survival rate, biomass accumulation, and number of haustoria. The promotion of this technology can effectively protect Sha'er's Deer Antler Grass, prevent the consumption of wild resources, and ensure the supply of Sha'er's Deer Antler Grass in the market.

[0093] In the cultivation management, the conditions of supplementary lighting and rooting agents were investigated, and the results are shown in Table 2.

[0094] Table 2 Effects of supplementary light and rooting agents on the growth indexes of Sachsengrass at the cotyledon stage ( n=10)

[0095]

[0096] Note: Different lowercase letters in the same column indicate significant differences, P < 0.05.

[0097] LL is a weak light control, i.e., the natural light intensity in the laboratory (500-1500 Lux); HL is a supplementary light treatment, i.e., an LED supplementary light is arranged about 40 cm above the non-woven bag for supplementary light (5000-8000 Lux). W1 is to pour seaweed concentrated nutrient solution (diluted 200 times) once a day, 40 mL per bag each time; W2 is to pour seaweed concentrated nutrient solution (diluted 200 times) once every two days, 40 mL per bag each time. R0 is a distilled water control, treated once a week; R15 is 15 mg / L rooting powder, treated once a week.

[0098] From the results of variance analysis in Table 2, except that the nutrient solution had no significant effect on the number of lateral roots of S. serrata in the cotyledon stage, the presence or absence of supplementary light, rooting powder and nutrient solution treatments all had significant effects on the growth of leaves and roots of S. serrata, especially the effect of supplementary light, which mostly reached the level of P<0.001. The interactions among the three treatments also had significant effects. For the effect on root length, the influence of each factor was rooting powder>supplementary light>nutrient solution, while the influence of each factor on other indicators was supplementary light>rooting powder>nutrient solution.

[0099] Example 3

[0100] Experimental study on the effects of different fertilizer treatments on the growth of Pterocarpus shabby

[0101] A method for planting saburo velvet grass by composite seedling cultivation is carried out according to the following steps:

[0102] (1) Selection of seedling container

[0103] A small non-woven bag with a specification of 4cm×12cm (diameter×height) was selected for the composite seedling cultivation of Pterocarya serrata and Digitaria tangutica.

[0104] (2) Matrix treatment

[0105] Choose garden soil, nutrient soil, fine sand and vermiculite, the ratio of mountain soil: nutrient soil: fine sand: vermiculite = 1:2:1:1.

[0106] (3) Treatment of Pterocarpus serrata seeds

[0107] The seeds of P. shabby were soaked in 800 mg / L gibberellin solution for 24 h, disinfected with mercuric chloride for 8 min, washed with distilled water and dried.

[0108] (4) Seeding of Deer Antler Grass and Crabgrass

[0109] Prioritize planting Sachsengrass seeds, sow 20 seeds in each seedling container, and sow crabgrass seeds one month later, sow 3 seeds in each seedling container.

[0110] (5) Water management

[0111] After sowing, water with a micro-sprinkler every 1 to 2 days, observe frequently, and ensure sufficient water supply during germination, so that the substrate is moist but not waterlogged. After the seedlings grow stably, water once every 2 to 4 days according to weather conditions, and each watering should moisten the substrate thoroughly. Observe the soil moisture once at 8:00 and 17:00 every day, and keep the relative moisture content of the soil in each treatment at 75% to 80%.

[0112] (3) Seedling management

[0113] Five treatment groups were set up: blank control (CK, i.e., equal amount of water treatment), seaweed nutrient solution treatment (HZ), MS culture solution treatment (MS), low concentration nitrogen, phosphorus and potassium treatment LN (seaweed nutrient solution + urea 0.5g / L + potassium dihydrogen phosphate 0.2g / L mixed solution) and high concentration nitrogen, phosphorus and potassium treatment HN (seaweed nutrient solution + urea 2g / L + potassium dihydrogen phosphate 0.8g / L mixed solution), with 15 non-woven bags in each treatment group. , managed uniformly in the greenhouse of Nanjing Agricultural University. The first treatment was carried out at around 6:00 pm after the seedlings emerged, with about 40mL per bag each time, and this period was continued, with treatment once every 7 days, for a total of 63 days. The results of the investigation are shown in Table 3.

[0114] Table 3 Effects of different fertilizer concentrations on the growth indexes of Pterocarpus sabdariffa ( n=15)

[0115]

[0116]

[0117] Note: Different lowercase letters in the same column indicate significant differences. “-” indicates missing data due to the death of P. shassoni seedlings.

[0118] The effects of different fertilization treatments on the growth indicators of Sha's Deer Antler Grass are shown in Table 3. Both HZ and LN treatments can promote the growth of Sha's Deer Antler Grass, but MS and HN treatments significantly inhibit the growth of Sha's Deer Antler Grass and even cause death under longer treatments. The root length and plant height of HZ were significantly higher than those of other treatments (p<0.05), and were 24.87% and 95.74% higher than those of CK, respectively. The leaf length and leaf width of Sha's Deer Antler Grass also showed significant differences among the three treatments of CK, HZ and LN (p<0.05). The leaf length and leaf width of HZ were significantly higher than those of CK and LN, and increased by 20.78% and 28.61% compared with CK. The above data show that HZ treatment has a significant promoting effect on the growth of Sha's Deer Antler Grass, while LN treatment can weaken the effect of HZ on the growth of Sha's Deer Antler Grass and even inhibit the growth of Sha's Deer Antler Grass.

[0119] Example 4

[0120] Experimental study on the effects of supplementary light and rooting agent on the growth indexes of Saba's velvet grass at the true leaf stage (wheat as host) A method for planting Saba's velvet grass by composite seedling cultivation, which is processed according to the following steps:

[0121] (1) Selection of seedling containers and substrate treatment

[0122] A small non-woven bag with a specification of 4cm×12cm (diameter×height) was selected for composite seedling cultivation of Pterocarya serrata and host wheat. Garden soil, nutrient soil (Cuiyunliang soil), fine sand and vermiculite were selected as the substrate, with the ratio of garden soil: nutrient soil: fine sand: vermiculite = 1:2:1:1.

[0123] (2) Seed treatment

[0124] The seeds of P. shavartz were soaked in 800 mg / L gibberellin solution for 24 hours, washed with distilled water for 3-4 times, and air-dried. The wheat seeds were air-dried and placed in a cool place for later use.

[0125] (3) Seed planting

[0126] Priority is given to planting the seeds of Sha's Deer Antler Grass, sowing 20 seeds in each seedling container, and sowing wheat seeds one month later, sowing 2 seeds in each seedling container.

[0127] (4) Cultivation management

[0128] An LED supplementary light (light intensity of 5000-8000 Lux) was arranged about 40 cm above the seedling container for supplementary lighting, and the supplementary lighting time was 7:30-19:30. At the same time, seaweed concentrated nutrient solution (diluted 200 times) was poured once a day, and 40 mL was poured into each bag of seedling container each time. The seedlings of P. saburoi were treated with rooting powder at a concentration of 15 mg / L, once a week, and the treatment lasted for 5 weeks.

[0129] This method of growing composite seedlings of Sha'er's antler grass and host wheat, through technical treatments such as substrate ratio, seedling container selection, LED light supplementary lighting, and fertilization, has resulted in extremely excellent indicators such as Sha'er's antler grass survival rate, biomass accumulation, and number of haustoria. The promotion of this technology can effectively protect Sha'er's antler grass, prevent the consumption of wild resources, and ensure the supply of Sha'er's antler grass in the market.

[0130] In the cultivation management, the conditions of supplementary lighting and rooting agents were investigated, and the results are shown in Table 4.

[0131] Table 4 Effects of supplementary light and rooting agents on the dry and fresh weights of Pterocarpus serrata at the true leaf stage ( n=10)

[0132]

[0133] Note: Wheat is the host plant. Different lowercase letters in the same column indicate significant differences, p < 0.05.

[0134] LL is a weak light control, i.e., the natural light intensity in the laboratory (500-1500 Lux); HL is a supplementary light treatment, i.e., an LED supplementary light is arranged about 40 cm above the non-woven bag for supplementary light (5000-8000 Lux). W1 is to pour seaweed concentrated nutrient solution (diluted 200 times) once a day, 40 mL per bag each time; W2 is to pour seaweed concentrated nutrient solution (diluted 200 times) once every two days, 40 mL per bag each time. R0 is a distilled water control, treated once a week; R15 is 15 mg / L rooting powder, treated once a week.

[0135] Under the condition of wheat as host, the effects of different light intensity, nutrient solution frequency and rooting powder concentration treatment on the fresh and dry weight of S. cerevisiae in the true leaf stage are shown in Table 4. The biomass and dry weight of S. cerevisiae in the supplementary light treatment were significantly higher than those in the non-supplementary light treatment. The maximum fresh weight and dry weight appeared in the HL+W1+R15 treatment (i.e., high light intensity supplementary light, 15 mg / L rooting powder once a week and nutrient solution watering once a day), in which the dry weight of this treatment was 14.75 times higher than that of the LL+W1+R0 treatment, and the fresh weight was 8.14 times higher than that of the LL+W1+R15 treatment. In the supplementary light treatment, the dry weight of the W1+R15 treatment was significantly higher than that of the W2+R15 treatment, while the dry weight of the S. cerevisiae in the W1+R0 treatment was 23.80% higher than that of the W2+R0 treatment, although the difference did not reach a significant level. This shows that increasing the frequency of nutrient solution is helpful to increase the biomass accumulation of S. cerevisiae in the true leaf stage. In the treatment without light supplementation, the maximum fresh and dry weight appeared in the W2+R15 treatment. The HL+W1+R15 treatment had the maximum dry weight, which was significantly higher than the treatment without light supplementation.

[0136] The above results show that supplementary light has a significant effect on the biomass accumulation of P. saburoides in the true leaf stage under the condition of wheat as host. Watering with seaweed concentrated nutrient solution once a day and treating with 15 mg / L rooting powder solution once a week on the basis of supplementary light can further promote the growth of P. saburoides.

[0137] The above contents are merely examples and illustrations of the structure of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, and these obvious replacement forms all belong to the protection scope of the present invention.

Claims

1. A composite seedling raising method of Pterocarpus shavarens-host, characterized in that: The following steps are involved: S1. Select a culture container: Select a non-woven bag or a plug tray as a culture container; S2. Substrate treatment: Take garden soil, nutrient soil, fine sand and vermiculite, mix them in a volume ratio of 1:1.5 to 2:1:1 as the substrate, and put them into the culture container; S3. Treatment of P. saburoides seeds: Select seeds of P. saburoides with full grains, soak them in gibberellin for 24 to 30 hours, wash them with distilled water for 3 to 4 times, and sow them immediately after natural air drying; S4. Sowing: Before sowing, first water the substrate to moisten thoroughly; the S3 treated velvet grass seeds and fine sand in a volume ratio of 1: 8 to 20 ratio after mixing, and then evenly sowed on the soil surface of the substrate, with a micro-sprinkler to moisten the soil surface; S5. Planting host: Sow wheat seeds or crabgrass seeds in the substrate sown with S. serrata, or sow wheat seeds and crabgrass seeds at the same time; S6. Light and nutrient management: When the safflower grass grows to the cotyledon stage and true leaf stage, it needs to be cultivated under high light intensity conditions; For the Sha's Deer Antler Grass in the cotyledon stage and true leaf stage, seaweed nutrient solution and rooting powder should be applied.

2. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In the sowing step of S4, the following steps are also included: S41. Water management: After sowing the velvet antler grass seeds on the soil surface of the substrate, water 1 to 2 times every 1 to 2 days using a micro sprinkler.

3. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In each non-woven bag or each cell, the sowing ratio of the sabdariffa seeds to the wheat seeds or crabgrass seeds is 8-10:

1.

4. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1 or 3, characterized in that: In S5, the time for planting host wheat or crabgrass should be at least one month after sowing the safflower.

5. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In S2 and S4, the fine sand is river sand, and the fine sand is sieved through a 20-40 mesh sieve.

6. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In S2, 3-5 days before formal sowing, the carbendazim solution is diluted into water at a volume ratio of 1:2000-2500, and the substrate is irrigated with the diluted carbendazim aqueous solution to wet it thoroughly.

7. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In S3, the seeds of P. saburoi are soaked in gibberellin, and the concentration of the gibberellin solution is 800-1000 mg / L.

8. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In the light management of S6, the Salix babylonica in the cotyledon stage and true leaf stage needs to be cultivated under high light intensity conditions, and the high light intensity conditions are 5000-10000 Lux; if the natural light is not enough, supplementary light treatment is required.

9. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: In the nutrient management of S6, the seaweed nutrient solution is a 200-fold dilution of concentrated seaweed nutrient solution, and the irrigation frequency is once a day; the concentration of the rooting powder is 15-18 mg / L, and the irrigation frequency is once a week.

10. The composite seedling raising method of Pterocarpus shavarens-host according to claim 1, characterized in that: As the host of the shamrock grass, the wheat is an annual or biennial herbaceous plant of the genus Triticum in the Poaceae family; the crabgrass is an annual herbaceous plant of the genus Crabgrass in the Poaceae family; Planting the host plant one month after sowing the safflower can: Sow wheat seeds and crabgrass seeds at the same time; Or you can sow wheat seeds first, and then sow crabgrass seeds after 10-20 days; Or you can sow crabgrass seeds first, and then sow wheat seeds 10-20 days later; Or just broadcast wheat seeds or crabgrass seeds.

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