Method for producing rice protein peptide and rice syrup through multistage enzymolysis
Through multi-level enzymatic lysis technology and chromatographic separation technology, the problems of high pollution, low quality and low separation efficiency in the existing rice protein processing technology were solved, and the production of high-purity rice protein peptides and rice syrup was achieved, which improved the rice Quality and added value of deep-processed products.
Patent Information
- Application Number
- CN202411604672.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-12
- Publication Date
- 2025-02-11
AI Technical Summary
In the existing rice protein processing technology, chemical methods have high pollution, low quality, low separation efficiency and insufficient purity of physical methods, and insufficient enzymatic reaction of biological enzymatics, resulting in low protein extraction rate and purity.
Using multi-stage enzymatic lysis technology, rice is first homogenized into slurry through high pressure, and then enzymatic lysis is used using amylase and protease to generate rice syrup and rice protein peptides, and high-purity rice protein is isolated by chromatography separation technology. Peptides and rice syrup.
It improves the purity and quality of rice protein peptides and rice syrup, solves the problem of low protein extraction rate and purity, and enhances the added value of deep-processed rice products.
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Figure CN119410739A_ABST
Abstract
Description
Technical Field
[0001] The invention relates to the technical field of rice deep processing, and in particular to a method for producing rice protein peptides and rice syrup by multi-stage enzymolysis. Background Art
[0002] Rice protein is considered a high-quality plant protein resource due to its reasonable amino acid composition, high bioavailability and low allergenicity. The hydrolyzed peptides of rice protein have biological activities such as lowering blood pressure and cholesterol, which makes it not only have broad application prospects in the food industry, but also show great potential in the fields of health food and medicine.
[0003] There are usually chemical methods, biological enzymatic methods and physical methods to extract rice protein from rice. The defects existing in the existing rice protein processing technology are:
[0004] 1. The chemical method uses the alkali-soluble and acid-precipitated properties of rice protein to extract rice protein. The chemical method is the most mature. The chemical method generally uses alkali solution to remove the protein in rice, obtains rice starch and clear liquid by centrifugal separation, and recovers rice protein by acid precipitation of the clear liquid. This process is highly polluting, and the quality of the obtained rice protein is not high. At the same time, there are disadvantages such as protein denaturation and high cost.
[0005] 2. Physical method uses high pressure homogenization to destroy the rice structure, and then uses the density difference of rice starch and rice protein to separate. Although this method retains the original characteristics of rice protein well, the separation efficiency of the two is not high, resulting in the purity of rice protein being not high enough, and more rice starch and other impurities remaining.
[0006] 3. The biological enzymatic hydrolysis method has greater advantages due to its mild process conditions, strong specificity, less protein damage, and controllable reaction conditions. In the enzymatic production of rice protein, non-rice protein components can be hydrolyzed by enzymes, leaving rice protein, and impurities can be separated by filtration or centrifugation, which is the non-protease method. Rice protein can also be hydrolyzed by enzymes to make rice protein soluble protein, and then extracted, which is the protease method. The main problem with the enzymatic extraction of rice protein is that the enzymatic reaction is not thorough enough, resulting in low protein extraction rate or low protein purity. Summary of the invention
[0007] The object of the present invention is to overcome the deficiencies in the prior art and provide a method for producing rice protein peptides and rice syrup by multi-stage enzymolysis. The present invention first processes rice into slurry by high pressure homogenization, then enzymolyzes the rice slurry into rice syrup and rice protein peptides by amylase and protease, and effectively separates the rice protein peptides and rice syrup by chromatographic separation technology, thereby producing rice protein peptides and rice syrup products with higher purity and better quality, and improving the added value of deep-processed rice products.
[0008] To achieve the above purpose, the technical solution designed by the present invention is as follows:
[0009] The invention provides a method for producing rice protein peptides and rice syrup by multi-stage enzymolysis, comprising the following steps:
[0010] (1) grinding rice, adding water and mixing, and performing high-pressure homogenization to obtain a first material;
[0011] (2) adjusting the pH of the first material, adding medium-temperature amylase, and performing enzymolysis to obtain a second material;
[0012] (3) cooling the second material and adjusting the pH, adding a composite protease, and performing enzymolysis to obtain a third material;
[0013] (4) subjecting the third material to membrane separation to remove insoluble impurities to obtain a fourth material;
[0014] (5) subjecting the fourth material to chromatographic separation to obtain a rice protein peptide feed liquid and a rice syrup feed liquid, respectively;
[0015] (6) obtaining rice protein peptide after the rice protein peptide feed liquid is dried, wherein the protein content in the rice protein peptide powder is 93.6-94.4%;
[0016] The rice syrup solution is concentrated to obtain glucose syrup with a DE value of 20 to 28.
[0017] Furthermore, in the step (1), the mass ratio of rice to water is 1:1.3-2.4, and the pressure of high-pressure homogenization is 12-16 MPa.
[0018] Furthermore, the mass ratio of the rice to water is 1:1.75-2.
[0019] Furthermore, in the step (2), sodium carbonate is used to adjust the pH of the first material to 5.6-6.2, the activity of the added medium-temperature protease is 200U, and the enzymatic hydrolysis conditions are 76-84°C and 1.5h; the DE value of the second material is 20.1-25.3.
[0020] Furthermore, in step (3), the temperature is lowered to 36-38° C., and the pH is adjusted to 6.6-6.8 using sodium carbonate;
[0021] The composite protease is a combination of trypsin and alkaline protease, wherein the enzymatic activity of trypsin is 200U, the enzymatic activity of alkaline protease is 200U, and the enzymolysis time is 3 to 4 hours.
[0022] Furthermore, in step (4), the filtration pore size of the membrane separation is 0.2 to 0.8 μm.
[0023] Furthermore, in the step (5), the chromatographic separation conditions are: the chromatogram is a simulated moving bed, six-column chromatography, the feed is 0.85-0.92BV, the circulation is 1.4-2.2BV, the elution material-water ratio is 1:1.2-1.5, the elution flow rate is 0.16-0.24BV, the discharge time is 10-15min, the single column volume is multiplied by 6, and the stationary phase is polyacrylamide.
[0024] Furthermore, the feed volume is 0.85 BV, the circulation volume is 1.4 BV, the elution material-water ratio is 1:1.3, the elution flow rate is 0.24 BV, and the discharge time is 10 min.
[0025] Furthermore, in the step (6), the drying method is spray drying, and the concentration method is MVR concentration; the DE value of the glucose syrup is 20.1-25.3.
[0026] Furthermore, in step (6), the rice syrup liquid can also be saccharified into glucose material by adding saccharifying enzyme, and then undergoing ion exchange and concentration to produce rice high-inverted sugar syrup.
[0027] Principle of the present invention:
[0028] 1. After the rice of the present invention is treated by high pressure homogenization, the solubility and swelling rate of the starch in the rice increase, and after adding amylase, the amylase hydrolyzes the starch in the rice into sugar molecules such as glucose, maltose, and dextrin; afterwards, after adding composite protease (trypsin and alkaline protease), the rice protein is hydrolyzed to generate protein peptides, so that the protein in the rice is present in the material in the form of small molecules. The mixed material is separated by chromatography to obtain higher purity sugar liquid and rice protein peptide products respectively.
[0029] 2. Chromatographic separation technology is a method for separating different substances based on the difference in the distribution coefficient between the stationary phase and the mobile phase. In the present invention, rice is physically crushed and bio-enzymatically hydrolyzed to obtain small molecule sugars and proteins. There is an obvious molecular weight difference between the hydrolyzed sugar and protein peptide molecules. The syrup molecules are relatively small and move relatively fast in the chromatographic resin with the mobile phase, while the protein peptide molecules are relatively large and migrate relatively slowly in the chromatogram. After feeding and circulation, the two are distributed in different chromatographic regions, and the syrup is pushed out of the system by the mixed phase feed, and the protein peptide is eluted out of the system by deionized water, thereby achieving continuous and effective separation of the two.
[0030] Beneficial effects of the present invention:
[0031] 1. After mechanical homogenization, multi-stage enzymatic hydrolysis technology is used to produce rice protein peptides and rice syrup, and the extraction of rice protein is more thorough.
[0032] 2. The process conditions for producing rice protein peptides using multi-stage enzymatic hydrolysis are milder, with less protein damage and better protein quality.
[0033] 3. Rice protein peptides and rice syrup are separated by chromatography, and the rice protein peptides produced are of higher purity and better product quality.
[0034] 4. The present invention combines physical methods to process rice raw materials and produces rice protein peptides by enzymatic method, thereby solving the problem of low protein purity in the rice protein extraction process and also solving the problem of protein residue in rice syrup due to incomplete separation of soluble protein in rice starch sugar production. BRIEF DESCRIPTION OF THE DRAWINGS
[0035] Figure 1 The present invention is a process flow chart of a method for producing rice protein peptides and rice syrup by multi-stage enzymatic hydrolysis. DETAILED DESCRIPTION
[0036] The present invention is further described in detail below in conjunction with specific embodiments so that those skilled in the art can understand.
[0037] Example 1
[0038] A method for producing rice protein peptides and rice syrup by multi-stage enzymolysis comprises the following steps:
[0039] 1. Grind 200 kg of rice to 80 mesh, then add 400 kg of water, mix well, pour into a high-pressure homogenizer for processing, the homogenization pressure is 16 MPa, and control the homogenized material to have a particle size of more than 80% below 7.2 μm under a particle size analyzer, as the first material.
[0040] 2. Add sodium carbonate to the first material to adjust the pH to 5.6-5.8, add 40 mL of medium-temperature amylase (the activity of medium-temperature amylase is 20,000 U / mL) to the first material, and then heat with stirring until the temperature of the first material rises to 84°C, stop stirring, keep warm for enzymolysis for 1.5 hours, take a sample after enzymolysis and measure the DE value to be 25.3, and obtain the second material.
[0041] 3. Cool the second material to 38°C, add solid sodium carbonate to adjust the pH to 6.8, add 4 mL of trypsin (the activity of trypsin is 200,000 U / mL) and 16 mL of alkaline protease (the activity of alkaline protease is 200,000 U / mL) to the second material, stir for 15 minutes and then keep warm for enzymolysis for 3 hours to obtain the third material.
[0042] 4. The third material obtained in step 3 is separated by a tubular membrane with a filtration pore size of 0.2 to 0.8 μm to remove insoluble impurities to obtain a fourth material.
[0043] 5. The fourth material is passed through a chromatographic separation device for continuous separation, and the chromatographic separation conditions are as follows: the chromatogram is a simulated moving bed, six column chromatography, the stationary phase is polyacrylamide, the feed is 0.89BV, the circulation is 2.2BV, the elution material-water ratio is 1: 1.5, the elution flow rate is 0.18BV, and the elution time is 12min. The fourth material treatment is completed to obtain rice protein peptide feed liquid and rice syrup feed liquid.
[0044] 6. The rice protein peptide feed liquid obtained 3.2 kilograms of rice protein peptide powder after spray drying, and the protein peptide powder protein content was 93.6%;
[0045] The rice syrup liquid can be directly concentrated by MVR to obtain a glucose syrup with a concentration of 70% and a DE value of 25.3; or the rice syrup liquid is heated to 60° C. and then 27 mL of a composite saccharifying enzyme (the activity of the composite saccharifying enzyme is 150,000 U / mL) is added, stirred for 10 min, and kept warm for 46 h, and then concentrated by ion exchange and MVR to obtain a 60% rice high invert syrup.
[0046] Example 2
[0047] A method for producing rice protein peptides and rice syrup by multi-stage enzymolysis comprises the following steps:
[0048] 1. Grind 200 kg of rice to 80 mesh, then add 400 kg of water, mix well, pour into a high-pressure homogenizer for processing, the homogenization pressure is 12 MPa, and control the homogenized material to have a particle size of more than 80% below 9.5 μm under a particle size analyzer, as the first material.
[0049] 2. Add sodium carbonate to the first material to adjust the pH to 6.0-6.2, add 30 mL of medium-temperature amylase (the activity of medium-temperature amylase is 200,000 U / mL) to the first material, and then heat with stirring until the material temperature rises to 80°C, stop stirring, keep warm for enzymolysis for 1.5 hours, take a sample after enzymolysis and measure the DE value to be 21.5, and obtain the second material.
[0050] 3. Cool the second material to 36°C, add sodium carbonate to adjust the pH to 6.8, add 5 mL of trypsin (the activity of trypsin is 200,000 U / mL) and 20 mL of alkaline protease (the activity of alkaline protease is 200,000 U / mL) to the second material, stir for 15 minutes and then keep warm for enzymolysis for 3 hours to obtain the third material.
[0051] 4. The third material obtained in step 3 is separated by a tubular membrane with a filtration pore size of 0.2 to 0.8 μm to remove insoluble impurities to obtain a fourth material.
[0052] 5. The fourth material is passed through a chromatographic separation device for continuous separation, and the chromatographic separation conditions are: the chromatogram is a simulated moving bed, six column chromatography, the stationary phase is polyacrylamide, the feed is 0.92BV, the circulation is 1.5BV, the elution material-water ratio is 1: 1.2, the elution flow rate is 0.16BV, and the discharging time is 15min. The fourth material treatment is completed to obtain rice protein peptide feed liquid and rice syrup feed liquid.
[0053] 6. The rice protein peptide liquid was spray dried to obtain 2.6 kg of finished protein peptide powder, the protein content of which was 92.5%.
[0054] The rice syrup liquid can be directly concentrated by MVR to obtain a glucose syrup with a concentration of 70% and a DE value of 21.5; or the rice syrup liquid is heated to 60° C. and then 27 mL of a composite saccharifying enzyme (the activity of the composite saccharifying enzyme is 150,000 U / mL) is added, stirred for 10 min, and then kept warm for 46 h, and then concentrated by ion exchange and MVR to obtain a 60% rice high invert syrup.
[0055] Example 3
[0056] A method for producing rice protein peptides and rice syrup by multi-stage enzymolysis comprises the following steps:
[0057] 1. Grind 200 kg of rice to 80 mesh, then add 350 kg of water, mix well, pour into a high-pressure homogenizer for processing, the homogenization pressure is 14 MPa, and control the homogenized material to have a particle size of more than 80% below 9.1 μm under a particle size analyzer, as the first material.
[0058] 2. Add sodium carbonate to the first material to adjust the pH to 5.9-6.1, add 30 mL of medium-temperature amylase (the activity of medium-temperature amylase is 200,000 U / mL) to the first material, and then heat with stirring until the material temperature rises to 76°C, stop stirring, keep warm for enzymolysis for 1.5 hours, take a sample after enzymolysis and measure the DE value to be 20.1, and obtain the second material.
[0059] 3. Cool the second material to 36°C, add sodium carbonate to adjust the pH to 6.6, add 6 mL of trypsin (the activity of trypsin is 200,000 U / mL) and 24 mL of alkaline protease (the activity of alkaline protease is 200,000 U / mL) to the second material, stir for 15 minutes and then keep warm for enzymolysis for 4 hours to obtain the third material.
[0060] 4. The third material obtained in step 3 is separated by a tubular membrane with a filtration pore size of 0.2 to 0.8 μm to remove insoluble impurities to obtain a fourth material.
[0061] 5, the 4th material is passed through chromatographic separation device, and is separated continuously, and the chromatographic separation condition is that chromatogram is simulated moving bed, six column chromatography, and stationary phase is polyacrylamide, and charging is 0.85BV, circulation 1.4BV, elution material-water ratio is 1: 1.3, elution flow rate 0.24BV, discharging time 10min, single column amount is multiplied by 6, and chromatogram is simulated moving bed, six column chromatography, and stationary phase is polyacrylamide.The 4th material treatment obtains rice protein peptide feed liquid and rice syrup feed liquid after completing.
[0062] 6. The rice protein peptide liquid was spray-dried to obtain 2.8 kg of finished protein peptide powder, the protein content of which was 94.4%.
[0063] The rice syrup liquid can be directly concentrated by MVR to obtain a glucose syrup with a concentration of 70% and a DE value of 20.1; or the rice syrup liquid is heated to 60° C. and then 27 mL of a composite saccharifying enzyme (the activity of the composite saccharifying enzyme is 1500000 U / mL) is added, stirred for 10 min, and then kept warm for 46 h, and then concentrated by ion exchange and MVR to obtain a 60% rice high invert syrup.
[0064] Although the above embodiments have been described in detail, they are only a part of the embodiments of the present invention, not all of them. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all belong to the protection scope of the present invention.
Claims
1. A method for producing rice protein peptides and rice syrup by multi-stage enzymolysis, characterized in that: The following steps are involved: (1) grinding rice, adding water and mixing, and performing high-pressure homogenization to obtain a first material; (2) adjusting the pH of the first material, adding medium-temperature amylase, and performing enzymolysis to obtain a second material; (3) cooling the second material and adjusting the pH, adding a composite protease, and performing enzymolysis to obtain a third material; (4) subjecting the third material to membrane separation to remove insoluble impurities to obtain a fourth material; (5) subjecting the fourth material to chromatographic separation to obtain a rice protein peptide feed liquid and a rice syrup feed liquid, respectively; (6) obtaining rice protein peptide after the rice protein peptide feed liquid is dried, wherein the protein content in the rice protein peptide powder is 93.6-94.4%; The rice syrup solution is concentrated to obtain glucose syrup with a DE value of 20 to 28.
2. The method according to claim 1, characterized in that: In the step (1), the mass ratio of rice to water is 1:1.3-2.4, and the pressure of high-pressure homogenization is 12-16 MPa.
3. The method according to claim 2, characterized in that: The mass ratio of rice to water is 1:1.75-2.
4. The method according to claim 1, characterized in that: In the step (2), sodium carbonate is used to adjust the pH of the first material to 5.6-6.2, the activity of the medium-temperature protease added is 200U, and the enzymatic hydrolysis conditions are 76-84°C and 1.5h; the DE value of the second material is 20.1-25.
3.
5. The method according to claim 1, characterized in that: In the step (3), the temperature is lowered to 36-38° C., and the pH is adjusted to 6.6-6.8 using sodium carbonate; The composite protease is a combination of trypsin and alkaline protease, wherein the enzymatic activity of trypsin is 200U, the enzymatic activity of alkaline protease is 200U, and the enzymolysis time is 3 to 4 hours.
6. The method according to claim 1, characterized in that: In the step (4), the filtration pore size of the membrane separation is 0.2 to 0.8 μm.
7. The method according to claim 1, characterized in that: In the step (5), the chromatographic separation conditions are as follows: the chromatogram is a simulated moving bed, six-column chromatography, the stationary phase is polyacrylamide, the mobile phase is RO water, the feed is 0.7-0.92BV, the circulation is 1.2-2.2BV, the elution material-water ratio is 1:1.0-1.5, the elution flow rate is 0.10-0.24BV, and the discharge time is 8-15min.
8. The method according to claim 7, characterized in that: The feed volume is 0.85 BV, the circulation volume is 1.4 BV, the elution material-water ratio is 1:1.3, the elution flow rate is 0.24 BV, and the discharge time is 10 min.
9. The method according to claim 1, characterized in that: In the step (6), the drying method is spray drying, and the concentration method is MVR concentration; the DE value of the glucose syrup is 20.1-25.
3.
10. The method according to claim 1, characterized in that: In the step (6), the rice syrup liquid can also be saccharified into glucose material by adding saccharifying enzyme, and then undergoing ion exchange and concentration to produce rice high-inverted sugar syrup.