Xanthophyll antioxidant composition and preparation method thereof

Abstract

The invention relates to a lutein antioxidant composition and a preparation method thereof, and belongs to the technical field of cosmetics. Comprising the following raw materials: 5-10% of a humectant, 0.5-1% of glycerol tri (ethylhexoic acid) ester, 1-2% of glyceryl stearate citrate, 0.2-0.5% of ceteth-20, 0.5-1% of alginate oligosaccharide, 2-4% of xanthophyll, 0.3-0.7% of lactic acid, 0.3-0.6% of a preservative, 0.5-1% of a radix gentianae extract, 0.5-1% of a herba portulacae extract, 0.5-1% of a snow lotus herb extract and the balance of deuterium-depleted water. The composition prepared by the invention has excellent oxidation resistance and soothing and moisturizing properties.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics and relates to a lutein antioxidant composition and a preparation method thereof. Background Art

[0002] In modern society, with the significant increase in the use of electronic devices, including computers, smartphones and tablets, the screen time has been prolonged, resulting in more and more people suffering from eye fatigue (also known as visual fatigue). Eye fatigue is a type of eye discomfort caused by long-term staring at a screen or other fine work, and its symptoms include dry eyes, blurred vision, headaches, neck and shoulder pain, etc.

[0003] Therefore, there is an urgent need for a selenium-rich eye protection essence spray and a preparation method thereof that can solve the above problems.

[0004] Conventional lutein is not easily soluble in water, is unstable to light / heat, and is not easy to penetrate human skin. When applied to aqueous solutions, additional solubilizers need to be added to improve the solubilization effect of lutein in water. The resulting product contains many fine particles. Even after extrusion, there are still problems such as dark liquid color, poor appearance transparency, and low raw material utilization rate.

[0005] In view of this, the present invention hopes to provide an eye spray with stable performance, good appearance transparency and good anti-blue light repair effect. Summary of the invention

[0006] The object of the present invention is to provide a lutein antioxidant composition and a preparation method thereof, wherein the prepared composition has good antioxidant properties.

[0007] The purpose of the present invention can be achieved through the following technical solutions:

[0008] A lutein antioxidant composition, comprising the following raw materials by weight percentage:

[0009] 5-10% moisturizer, 0.5-1% triethylhexanoin, 1-2% glyceryl stearate citrate, 0.2-0.5% ceteth-20, 0.5-1% brown algae oligosaccharide, 2-4% lutein, 0.3-0.7% lactic acid, 0.3-0.6% preservative, 0.5-1% gentian extract, 0.5-1% purslane extract, 0.5-1% snow lotus extract, and the balance is deuterium-depleted water.

[0010] As a preferred technical solution of the present invention, the preservative is p-hydroxyacetophenone.

[0011] As a preferred technical solution of the present invention, the moisturizing agent is 1,3-propylene glycol, 2,3-butylene glycol, 1,2-hexanediol and 1,2-butylene glycol in a mass ratio of 1:1:1:1.

[0012] As a preferred technical solution of the present invention, the deuterium content of the deuterium-depleted water is <150 ppm.

[0013] As a preferred technical solution of the present invention, the lutein exists in the form of lutein liposomes, wherein the preparation process of the lutein liposomes is as follows:

[0014] Step 1, mixing lutein, lipid phase and solvent in a mass ratio of (0.3-0.6):100 to obtain a lutein lipid solution, removing the solvent by rotary evaporation, and continuing to dry for 30-35 minutes under a vacuum degree of 0.1 MPa to obtain a mixture A;

[0015] Step 2, mixing mixture A with phosphate buffer at 50-60° C., stirring at 300 r / min for 20-30 min, naturally cooling to room temperature, ultrasonically treating in an ice-water bath with stirring, and then centrifuging at 10000 r / min for 10 min to obtain mixture B, wherein the pH of the phosphate buffer is 6.5-7.5, and the mass ratio of mixture A to phosphate buffer is 1:0.5;

[0016] Step 3: Add polyglutamic acid to mixture B, stir at room temperature at a speed of 250 r / min for 5 to 8 hours, and extrude through a polycarbonate membrane with a pore size of 200 nm for 10 to 20 times to obtain the lutein liposome.

[0017] As a preferred technical solution of the present invention, the solvent is a mixture of chloroform and methanol in a volume ratio of 2:1.

[0018] As a preferred technical solution of the present invention, the lipid phase is composed of cholesterol and lecithin in a mass ratio of (0.2-0.5):1, and the mass concentration of lutein in the lutein lipid solution is 1-2%.

[0019] As a preferred technical solution of the present invention, in step 2, the power of the ultrasonic treatment is 500-650W, and the treatment time is 10-30min.

[0020] A method for preparing a lutein antioxidant composition comprises the following steps:

[0021] S1. Add the preservative to the moisturizer according to the mass percentage, heat to 65-75°C under vacuum conditions, stir at a speed of 100 r / min for 15-25 min, and then homogenize for 1 h to obtain phase A liquid;

[0022] S2, add triethylhexanoin, glyceryl stearate citrate and lactose to the phase A liquid in sequence, and continue stirring for 30 to 35 minutes at a constant speed to obtain the phase B liquid;

[0023] S3, mixing lutein liposomes, brown algae oligosaccharides, ceteth-20 and deuterium-depleted water at room temperature, then adding them to the phase B liquid, heating and stirring to obtain the phase C liquid;

[0024] S4. Evenly mix the gentian extract, purslane extract and snow lotus extract, then add them into the phase C liquid, heat to 35-45° C. under vacuum, stir at a speed of 100 r / min for 40-50 min, and obtain the lutein antioxidant composition after cooling.

[0025] As a preferred technical solution of the present invention, in step S3, the temperature of the heating and stirring is 50-60°C, the time is 10-20 minutes, and the rotation speed is 100 r / min.

[0026] 1,3-Propanediol can form a layer of moisture protection film on the skin surface, effectively preventing water loss, keeping the skin moist and soft, and promoting other ingredients in skin care products to penetrate into the skin; 1,2-Butanediol has a larger molecular particle size, is not easy to evaporate water, and can absorb more water. 1,2-Hexanediol can reduce the loss of skin moisture, help the skin moisturize, and assist in improving dry skin and flaking. 2,3-Butanediol can absorb moisture from the air and lock in moisture to keep the skin moisturized. Moisturizers are obtained by mixing polyols, so they also have the ability to resist microbial reproduction and can inhibit bacterial growth. Different alcohols have different moisturizing mechanisms and can complement each other to provide a more comprehensive and lasting moisturizing effect.

[0027] Glyceryl tri(ethylhexanoate) is a weakly polar emollient that has a light and dry skin feel and has good spreadability and breathability.

[0028] Glyceryl stearate citrate can form a protective film on the surface of the eye skin, lock in moisture and prevent moisture evaporation, thereby maintaining the moisture and softness of the eye skin. This softening effect helps relieve the dryness and tightness of the eye skin, making the eye skin more comfortable and smooth. At the same time, as an anionic surfactant, it can form a stable emulsion with other ingredients to improve the permeability and absorption efficiency of the composition.

[0029] Ceteth-20 is a surfactant that can reduce the surface tension of water, making other ingredients in the composition more easily dispersed and dissolved, thereby improving the stability and uniformity of the composition.

[0030] Brown algae oligosaccharides have good moisturizing properties and can provide lasting moisture nourishment to the skin around the eyes, relieving dryness and tightness of the skin around the eyes. At the same time, brown algae oligosaccharides have certain antioxidant and anti-inflammatory effects, which can remove free radicals and prevent aging and damage of the skin around the eyes.

[0031] Lactic acid has good moisturizing properties and can provide long-lasting moisture nourishment to the eye skin. It can absorb and retain moisture, prevent the eye skin from drying out and tightening, thereby maintaining the skin's moisture and softness. This has a positive effect on relieving problems such as eye fatigue and dryness.

[0032] Gentian extract has anti-inflammatory effects, can reduce eye inflammation, relieve symptoms such as eye congestion and redness, and can enhance the eye's defense capabilities, reduce the possibility of bacteria entering the eye, and protect eye health.

[0033] Purslane extract has a soothing effect and can relieve the sensitivity and discomfort of the skin around the eyes. It can provide lasting moisture nourishment to the skin around the eyes and prevent the skin around the eyes from becoming dry and tight. Purslane extract contains certain nutrients that can promote blood circulation around the eyes and relieve problems such as eye fatigue and dark circles.

[0034] Snow lotus extract can improve eye microcirculation, making the eyes feel more comfortable and relaxed. Snow lotus extract has anti-inflammatory effects and can relieve symptoms such as eye inflammation and swelling. Snow lotus extract contains rare alkaloids and phenolic acids, which can protect eye health and prevent the occurrence of eye diseases.

[0035] Gentiana extract, purslane extract and snow lotus extract all have soothing effects, which can relieve eye discomfort and fatigue. When they work together, they can produce a stronger soothing effect, making the skin around the eyes more relaxed and comfortable. This synergistic effect helps improve eye microcirculation and reduce eye fatigue and discomfort. Gentiana extract and snow lotus extract both have anti-inflammatory effects, which can reduce eye inflammation and swelling. When they work together, they can produce a stronger anti-inflammatory effect, which helps relieve problems such as eye inflammation and redness. Snow lotus extract also has certain moisturizing ingredients. Combined with purslane extract, it can further enhance the moisturizing effect and make the skin around the eyes softer and smoother.

[0036] Gentian extract, purslane extract and snow lotus extract each have unique effects, such as anti-inflammatory, soothing, moisturizing, etc. When they work together, they can comprehensively protect eye health.

[0037] Lutein is the main pigment in the macula of the human retina and is a natural antioxidant for the eye. Lutein liposomes can more effectively deliver lutein to the eye, helping the eye to squeeze out blue light, thereby avoiding damage to the eye caused by blue light. However, lutein has poor solubility, so using lutein as a liposome can improve its solubility and help better exert its antioxidant properties.

[0038] Lutein liposomes use lipid encapsulation technology, with cholesterol and lecithin as the lipid phase, and can encapsulate lutein and other eye protection ingredients in lipid microspheres, thereby improving their permeability and absorption efficiency. Cholesterol can regulate the fluidity of the liposome membrane, reduce membrane permeability, thereby reducing leakage and improving the stability of the liposome. Lecithin is one of the main components of the cell membrane, so it has good biocompatibility, which helps the liposome to better fuse with the cell membrane and improve the delivery efficiency. This allows the composition to reach the deep tissues of the eye more quickly and exert a better antioxidant eye protection effect.

[0039] Using the hydrophilic anionic polymer polyglutamic acid simultaneously can improve the water solubility and biocompatibility of liposome. Utilize the hydrophilicity of polyglutamic acid, in the lutein liposome preparation process, add polyglutamic acid therein, can make polyglutamic acid fully dispersed, in the mixing process, polyglutamic acid can interact with the liposome surface through the physical forces such as electrostatic action and hydrogen bond, thereby realize the embedding of lutein liposome, can significantly improve the liposome to the lutein encapsulation rate. Lutein liposome keeps the strong antioxidant effect of lutein at the same time, can resist the infringement of free radicals, thereby delays the aging process of eyes, and prevents the occurrence of eye lesions. Utilize the further embedding effect of polyglutamic acid, can protect lutein liposome from the influence of adverse factors such as light, heat, oxygen, thereby improve its chemical stability, enhance bioavailability, and the hydrophilicity and biocompatibility of polyglutamic acid contribute to the absorption and utilization of lutein liposome in vivo, thereby improve the bioavailability of lutein.

[0040] Beneficial effects of the present invention:

[0041] The invention prepares a composition with antioxidant function, which provides excellent antioxidant capacity through lutein liposomes, is assisted by gentian extract, purslane extract and snow lotus extract, and can exert antioxidant properties to the maximum extent after being mixed with deuterium-deficient water with the addition of moisturizers and other auxiliary agents. BRIEF DESCRIPTION OF THE DRAWINGS

[0042] In order to facilitate understanding by those skilled in the art, the present invention is further described below with reference to the accompanying drawings.

[0043] Figure 1 The negative images before and after the test in Example 1 (the left image is before the test, and the right image is after the test);

[0044] Figure 2 The pictures before and after the positive test in Example 1 (the left picture is before the test, and the right picture is after the test);

[0045] Figure 3 The pictures are before and after the test using the composition in Example 1 (the left picture is before the test, and the right picture is after the test). DETAILED DESCRIPTION

[0046] In order to further illustrate the technical means and effects adopted by the present invention to achieve the predetermined invention purpose, the specific implementation methods, structures, features and effects of the present invention are described in detail below in combination with the embodiments.

[0047] In the comparative examples and embodiments of the present invention:

[0048] The moisturizing agent is 1,3-propylene glycol, 2,3-butylene glycol, 1,2-hexanediol and 1,2-butylene glycol in a mass ratio of 1:1:1:1;

[0049] The deuterium content of low-deuterium water is <150ppm;

[0050] Glyceryl tri(ethylhexanoate): purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0051] Glyceryl stearate citrate: purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0052] Ceteth-20: purchased from Wuhan Rongcan Biotechnology Co., Ltd.;

[0053] Brown algae oligosaccharide: purchased from Hebei Qiansheng Biotechnology Co., Ltd.;

[0054] Lutein: purchased from Fufeng Snort Biotechnology Co., Ltd.;

[0055] Lactic acid: purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0056] Gentiana extract: purchased from Nanjing Recovery Biotechnology Co., Ltd.;

[0057] Portulaca oleracea extract: purchased from Fufeng Snotech Biotechnology Co., Ltd.

[0058] Snow lotus extract: purchased from Nanjing Recovery Biotechnology Co., Ltd.;

[0059] p-Hydroxyacetophenone: purchased from Shanghai Yuanye Biotechnology Co., Ltd.;

[0060] Cholesterol: purchased from Shanghai Yimiao Chemical Technology Co., Ltd.;

[0061] Lecithin: purchased from Wuhan Yuanqi Pharmaceutical Chemical Co., Ltd.

[0062] Polyglutamic acid: purchased from Hangzhou Linran Biotechnology Co., Ltd.

[0063] Example 1

[0064] The preparation process of lutein liposomes is as follows:

[0065] Step 1, mixing lutein in a mass ratio of 0.3:100, a lipid phase and a mixture of chloroform and methanol in a volume ratio of 2:1 to obtain a lutein lipid solution, removing the solvent by rotary evaporation, and continuing to dry for 30 minutes under a vacuum degree of 0.1 MPa to obtain a mixture A, wherein the lipid phase is composed of cholesterol and lecithin in a mass ratio of 0.2:1, the mass concentration of lutein in the lutein lipid solution is 1%, the pH of the phosphate buffer is 6.5, and the mass ratio of the mixture A to the phosphate buffer is 1:0.5;

[0066] Step 2: Mix the mixture A with the phosphate buffer at 50° C., stir at 300 r / min for 20 min, cool naturally to room temperature, and then ultrasonically treat in an ice water bath with stirring. After the mixture is centrifuged at 10000 r / min for 10 min to obtain a mixture B. The power of the ultrasonic treatment is 500 W, and the treatment time is 10 min.

[0067] Step 3: Add polyglutamic acid to mixture B, stir at room temperature at a speed of 250 r / min for 5 to 8 hours, and extrude 10 times through a polycarbonate membrane with a pore size of 200 nm to obtain the lutein liposome.

[0068] Preparation of lutein antioxidant composition:

[0069] S1. Add 0.3% p-hydroxyacetophenone to 5% moisturizer by mass percentage, heat to 65°C under vacuum, stir at 100 r / min for 15 min, and then homogenize for 1 h to obtain phase A liquid;

[0070] S2, 0.5% triethylhexanoin, 1% glyceryl stearate citrate and 0.3% lactic acid are added to the phase A liquid in sequence, and stirring is continued for 30 minutes at a constant speed to obtain the phase B liquid;

[0071] S3, 2% lutein liposomes, 0.5% brown algae oligosaccharides, 0.2% ceteth-20 and deuterated water were mixed evenly at room temperature, then added to the phase B liquid, heated to 50°C and stirred at a speed of 100 r / min for 10 min to obtain a phase C liquid;

[0072] S4. Evenly mix 0.5% gentian extract, 0.5% purslane extract and 0.5% snow lotus extract, then add them into the phase C liquid, heat to 35° C. under vacuum, stir at 100 r / min for 40 min, and obtain a lutein antioxidant composition after cooling.

[0073] Example 2

[0074] The preparation process of lutein liposomes is as follows:

[0075] Step 1, mixing lutein in a mass ratio of 0.5:100, a lipid phase and a mixture of chloroform and methanol in a volume ratio of 2:1 to obtain a lutein lipid solution, removing the solvent by rotary evaporation, and continuing to dry for 33 minutes under a vacuum degree of 0.1 MPa to obtain a mixture A, wherein the lipid phase is composed of cholesterol and lecithin in a mass ratio of 0.3:1, the mass concentration of lutein in the lutein lipid solution is 1.5%, the pH of the phosphate buffer is 7, and the mass ratio of the mixture A to the phosphate buffer is 1:0.5;

[0076] Step 2: Mix the mixture A with the phosphate buffer at 55° C., stir at 300 r / min for 25 min, cool naturally to room temperature, and then ultrasonically treat in an ice-water bath with stirring. After the mixture is centrifuged at 10,000 r / min for 10 min to obtain a mixture B. The power of the ultrasonic treatment is 600 W, and the treatment time is 20 min.

[0077] Step 3: Add polyglutamic acid to mixture B, stir at room temperature at a speed of 250 r / min for 6 hours, and extrude through a polycarbonate membrane with a pore size of 200 nm for 15 times to obtain the lutein liposome.

[0078] Preparation of lutein antioxidant composition:

[0079] S1. Add 0.5% p-hydroxyacetophenone to 8% moisturizer by mass percentage, heat to 70°C under vacuum, stir at 100 r / min for 20 min, and then homogenize for 1 h to obtain phase A liquid;

[0080] S2, 0.8% of triethylhexanoin, 1.5% of glyceryl stearate citrate and 0.5% of lactic acid are added to the phase A liquid in sequence, and stirring is continued for 32 minutes at a constant speed to obtain the phase B liquid;

[0081] S3, 3% lutein liposomes, 0.8% brown algae oligosaccharides, 0.3% ceteth-20 and deuterated water were mixed evenly at room temperature, then added to the phase B liquid, heated to 55°C and stirred at a speed of 100 r / min for 15 minutes to obtain a phase C liquid;

[0082] S4. Evenly mix 0.7% gentian extract, 0.7% purslane extract and 0.7% snow lotus extract, then add them into the phase C liquid, heat to 40°C under vacuum, stir at 100 r / min for 45 min, and obtain a lutein antioxidant composition after cooling.

[0083] Example 3

[0084] The preparation process of lutein liposomes is as follows:

[0085] Step 1, mixing lutein in a mass ratio of 0.6:100, a lipid phase and a mixture of chloroform and methanol in a volume ratio of 2:1 to obtain a lutein lipid solution, removing the solvent by rotary evaporation, and continuing to dry for 35 minutes under a vacuum degree of 0.1 MPa to obtain a mixture A, wherein the lipid phase is composed of cholesterol and lecithin in a mass ratio of 0.5:1, the mass concentration of lutein in the lutein lipid solution is 2%, the pH of the phosphate buffer is 7.5, and the mass ratio of the mixture A to the phosphate buffer is 1:0.5;

[0086] Step 2: Mix the mixture A with the phosphate buffer at 60° C., stir at 300 r / min for 30 min, cool naturally to room temperature, and then ultrasonically treat in an ice-water bath with stirring. After the mixture is centrifuged at 10,000 r / min for 10 min to obtain a mixture B. The power of the ultrasonic treatment is 650 W, and the treatment time is 30 min.

[0087] Step 3: Add polyglutamic acid to mixture B, stir at room temperature at a speed of 250 r / min for 8 hours, and extrude 20 times through a polycarbonate membrane with a pore size of 200 nm to obtain the lutein liposome.

[0088] Preparation of lutein antioxidant composition:

[0089] S1. Add 0.6% p-hydroxyacetophenone to 10% moisturizer by mass percentage, heat to 75°C under vacuum, stir at 100 r / min for 25 min, and then homogenize for 1 h to obtain phase A liquid;

[0090] S2, 1% triethylhexanoin, 2% glyceryl stearate citrate and 0.7% lactic acid are added to the phase A liquid in sequence, and stirring is continued for 35 minutes at a constant speed to obtain the phase B liquid;

[0091] S3, 4% lutein liposomes, 1% brown algae oligosaccharides, 0.5% ceteth-20 and deuterated water were mixed evenly at room temperature, then added to the phase B liquid, heated to 60°C and stirred at a speed of 100 r / min for 20 min to obtain a phase C liquid;

[0092] S4. Evenly mix 1% gentian extract, 1% purslane extract and 1% snow lotus extract, then add them into the phase C liquid, heat to 45° C. in vacuum, stir at 100 r / min for 50 min, and obtain a lutein antioxidant composition after cooling.

[0093] Comparative Example 1

[0094] The difference between Comparative Example 1 and Example 1 is that polyglutamic acid is not added when preparing lutein liposomes in Comparative Example 1, and the other operations are the same.

[0095] Comparative Example 2

[0096] The difference between Comparative Example 2 and Example 1 is that in Comparative Example 2, lutein is directly added without being processed by forming liposomes, and the other operations are the same.

[0097] Comparative Example 3

[0098] The difference between Comparative Example 3 and Example 1 is that in Comparative Example 3, only the gentian extract is added, and the purslane extract and the snow lotus extract are not added, and the other operations are the same.

[0099] 1. Irritation test:

[0100] According to the laboratory method (XYJC-SOP-CE-015 Chicken Embryo Chorioallantoic Membrane Test Operation Guide), the irritation of the composition prepared in Example 1 was tested;

[0101] Instruments and equipment: M3LY630T stereo microscope, SPF chicken embryos, JM176 fully automatic incubator;

[0102] Test substance: Sample: original solution of the composition prepared in Example 1; Negative control: 0.9% saline; Positive control group: 0.1 mol / L sodium hydroxide solution;

[0103] Calculation formula:

[0104] ES = (the sum of the degrees of bleeding, coagulation, and vascular melting observed in 6 chicken embryos) / 3, where ES ≤ 4 indicates no irritation, and ES ≥ 16 indicates strong irritation / corrosiveness. The test results are shown in Table 1:

[0105] Table 1

[0106] Group ES value Irritation classification Negative control group 0.00 Non-irritating Positive control group 18.74 Strong irritant / corrosive Example 1 2.00 Non-irritating

[0107] According to the test results, when the negative and positive controls of the test system meet the standards of the reference substance (negative control ES = 0.00, positive control ES = 18.74), when the sample is the stock solution prepared in Example 1, the ES of the sample is 2.00, indicating that the sample is non-irritating and has a certain mild effect. Figure 1-3 It can also be seen from the figure that the composition prepared by the present invention is non-irritating.

[0108] 2. Moisture test:

[0109] According to the laboratory method (XYJC-SOP-CE-022 Cosmetic Moisturizing Efficacy Test Method-Weighing Method), the moisture retention rate of Example 1 was tested;

[0110] Test substance: The sample group was the original test sample, and the positive control group was 3% glycerol.

[0111] Test steps:

[0112] Pre-treat the samples and positive controls, weigh a certain amount of the samples and positive controls and place them on a test glass plate, then place them in a constant temperature and humidity chamber (temperature 20°C, relative humidity 50±5%), and calculate the moisture retention rate of the samples and positive controls by measuring the ratio of the sample volume after the aqueous solution samples and positive controls have been placed for 1h, 2h, and 4h to the initial sample volume;

[0113] Moisture retention rate = (Mt-M0) / M

[0114] Where: Mt is the mass of the sample (positive control) and the plate after being placed for 1h, 2h, and 4h, M0 is the mass of the empty plate, M is the mass of the weighed sample (positive control), and the test result is * 100%. The results are shown in Table 2:

[0115] Table 2

[0116] Group 1h 2h 4h Positive control group 44.75±1.124 17.04±1.281 6.08±0.788 Example 1 65.14±1.550 40.41±1.178 8.38±0.569

[0117] According to the test results in Table 2, the moisturizing rates of the samples at 1h, 2h and 4h are 65.14%, 40.41% and 8.38% respectively. It can be seen that the moisturizing rates of the test samples at 1h, 2h and 4h are higher than those of the positive control, indicating that the sample has a certain moisturizing effect.

[0118] 3. According to the laboratory method (XYJC-SOP-CE-021 hyaluronidase inhibition rate method), Example 1 was tested;

[0119] The sample is the original solution of the sample in Example 1, the negative control is pure water, and the positive control is dipotassium glycyrrhizinate solution; four groups, ABCD, are set respectively, and 3 parallels are set in each group. Calcium chloride, buffer solution, sample solution, sodium hydroxide, acetic acid acetone and other solutions are added to the test tubes successively, mixed, and then placed in boiling water, ice water, and room temperature for reaction, and then P-DAB color developer is added to the four tubes. After the solution inside reacts completely, the absorbance value of each group is measured at a wavelength of 530nm.

[0120] Hyaluronidase inhibition rate = [(CD)-(AB)] / (CD)

[0121] Wherein: A is the absorbance of the reaction solution containing the sample and the enzyme, B is the absorbance of the reaction solution containing the sample and not containing the enzyme, C is the absorbance of the reaction solution containing the enzyme but not containing the sample, and D is the absorbance of the reaction solution excluding the sample and the enzyme. The obtained test results are * 100%. The results are shown in Table 3:

[0122] Table 3

[0123] Group Test results P-value Negative control group -8.129±1.835 / Positive control group 83.643±1.956 <0.05 Example 1 41.038±3.575 <0.05

[0124] According to the above test results, when the experimental system is guaranteed to be effective (the hyaluronidase inhibition rate of the positive control is greater than 50%), when the sample is the original solution of the embodiment, the hyaluronidase inhibition rate is 41.038%, which is significantly different from that of the negative control (P<0.05), indicating that the sample has a certain soothing effect.

[0125] 4. Antioxidant test:

[0126] According to the laboratory method (XYJC-SOP-CE-018 free radical (DPPH) scavenging test method), the antioxidant effects of the embodiments and comparative examples were tested.

[0127] Test substance: Sample: The samples of the examples and comparative examples were diluted into 10% aqueous solution, the negative control was pure water, and the positive control was 0.1% vitamin E solution.

[0128] Test method: Set up sample tubes, blank tubes, positive control tubes (T) and sample, blank, positive control background (T0), set up 3 parallels for each group, add ethanol solution, sample solution and DPPH solution respectively, let stand at room temperature for 30 minutes, and after the solution in the test tube reacts completely, measure the absorbance value of each group at a wavelength of 517nm.

[0129] Calculation formula:

[0130] Free radical scavenging rate = 1-(T-T0) / (C-C0)

[0131] Where: T is the absorbance of the test tube, that is, the absorbance of the solution after the test object reacts with DPPH, T0 is the background absorbance of the test object, C is the average of 3 times the absorbance of the DPPH tube, that is, the absorbance of the DPPH solution when no sample is added, C0 is the background absorbance of the solvent, and the obtained test results * 100%, see Table 4:

[0132] Table 4

[0133] Group Test results P-value Negative control group -9.716±0.410 / Positive control group 81.219±1.694 <0.05 Example 1 13.971±0.900 <0.05 Example 2 14.242±0.673 <0.05 Example 3 15.020±0.025 <0.05 Comparative Example 1 12.041±0.504 <0.05 Comparative Example 2 10.556±0.901 <0.05 Comparative Example 3 12.143±0.047 <0.05

[0134] The test results show that: under the premise of ensuring the effectiveness of the experimental reaction system (free radical scavenging rate of the positive control >50%), when the sample concentration is 10%, the free radical scavenging rate is 13.971%, which is significantly different from the negative control (P<0.05), indicating that the sample has an antioxidant effect.

[0135] The above description is only a preferred embodiment of the present invention and does not limit the present invention in any form. Although the present invention has been disclosed as a preferred embodiment as above, it is not used to limit the present invention. Any technical personnel in this field can make some changes or modify the technical contents disclosed above into equivalent embodiments without departing from the scope of the technical solution of the present invention. However, any brief modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention without departing from the content of the technical solution of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A lutein antioxidant composition, characterized in that: In terms of weight percentage, the following raw materials are included: 5-10% moisturizer, 0.5-1% triethylhexanoin, 1-2% glyceryl stearate citrate, 0.2-0.5% ceteth-20, 0.5-1% brown algae oligosaccharide, 2-4% lutein, 0.3-0.7% lactic acid, 0.3-0.6% preservative, 0.5-1% gentian extract, 0.5-1% purslane extract, 0.5-1% snow lotus extract, and the balance is deuterium-depleted water.

2. The lutein antioxidant composition according to claim 1, characterized in that: The preservative is p-hydroxyacetophenone.

3. The lutein antioxidant composition according to claim 1, characterized in that: The moisturizing agent is 1,3-propylene glycol, 2,3-butylene glycol, 1,2-hexanediol and 1,2-butylene glycol in a mass ratio of 1:1:1:

1.

4. The lutein antioxidant composition according to claim 1, characterized in that: The deuterium content of the deuterium-depleted water is <150 ppm.

5. The lutein antioxidant composition according to claim 1, characterized in that: The lutein exists in the form of lutein liposomes, wherein the preparation process of the lutein liposomes is as follows: Step 1, mixing lutein, lipid phase and solvent in a mass ratio of (0.3-0.6):100 to obtain a lutein lipid solution, removing the solvent by rotary evaporation, and continuing to dry for 30-35 minutes under a vacuum degree of 0.1 MPa to obtain a mixture A; Step 2, mixing mixture A with phosphate buffer at 50-60° C., stirring at 300 r / min for 20-30 min, naturally cooling to room temperature, ultrasonically treating in an ice-water bath with stirring, and then centrifuging at 10000 r / min for 10 min to obtain mixture B, wherein the pH of the phosphate buffer is 6.5-7.5, and the mass ratio of mixture A to phosphate buffer is 1:0.5; Step 3: Add polyglutamic acid to mixture B, stir at room temperature at a speed of 250 r / min for 5 to 8 hours, and extrude through a polycarbonate membrane with a pore size of 200 nm for 10 to 20 times to obtain the lutein liposome.

6. The lutein antioxidant composition according to claim 5, characterized in that: The solvent is a mixture of chloroform and methanol in a volume ratio of 2:

1.

7. The lutein antioxidant composition according to claim 5, characterized in that: In step 1, the lipid phase is composed of cholesterol and phosphatidylcholine in a mass ratio of (0.2-0.5):1, and the mass concentration of lutein in the lutein lipid solution is 1-2%.

8. The lutein antioxidant composition according to claim 5, characterized in that: In step 2, the power of the ultrasonic treatment is 500-650W, and the treatment time is 10-30min.

9. A method for preparing the lutein antioxidant composition according to any one of claims 1 to 8, characterized in that: The following steps are involved: S1. Add the preservative to the moisturizer according to the mass percentage, heat to 65-75°C under vacuum conditions, stir at a speed of 100 r / min for 15-25 min, and then homogenize for 1 h to obtain phase A liquid; S2, add triethylhexanoin, glyceryl stearate citrate and lactose to the phase A liquid in sequence, and continue stirring for 30 to 35 minutes at a constant speed to obtain the phase B liquid; S3, mixing lutein liposomes, brown algae oligosaccharides, ceteth-20 and deuterium-depleted water at room temperature, then adding them to the phase B liquid, heating and stirring to obtain the phase C liquid; S4. Evenly mix the gentian extract, purslane extract and snow lotus extract, then add them into the phase C liquid, heat to 35-45° C. under vacuum, stir at a speed of 100 r / min for 40-50 min, and obtain the lutein antioxidant composition after cooling.

10. The method for preparing the lutein antioxidant composition according to claim 9, characterized in that: In step S3, the heating and stirring is performed at a temperature of 50 to 60°C, a time of 10 to 20 minutes, and a rotation speed of 100 r / min.

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