White tomato whitening and repairing composition as well as preparation method and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a white tomato whitening and repairing composition as well as a preparation method and application thereof. The composition comprises the following components in percentage by mass: 68-85% of jojoba oil, 10-25% of squalane and the balance of white tomato extract, wherein the total percentage is 100%. The white tomatoes are rich in colorless carotenoids (such as phytoene and tetrahydrolycopene), and have excellent antioxidant and whitening capabilities: melanin formation is inhibited, and the colorless carotenoids can inhibit tyrosinase activity, reduce melanin generation and fundamentally whiten the skin. Pigmentation caused by ultraviolet rays can be relieved through the anti-oxidation effect, and the color spots are prevented and faded. The skin color can be brightened after continuous use, so that the skin presents natural luster.

Description

Technical Field

[0001] The invention relates to the technical field of daily chemicals, and in particular to a white tomato whitening and repairing composition and a preparation method and application thereof. Background Art

[0002] As the boundary organ between the human body and the environment, human skin has been constantly affected by free radicals from the outside in and from the inside out. Ultraviolet radiation is the main factor leading to the formation of free radicals, especially reactive oxygen species (ROS) in human skin. 80% of visible aging phenomena are caused by sun damage, including: dull skin color, pigmentation, sun spots, loss of skin elasticity, and rough skin and wrinkles.

[0003] Nutrients play an important role in maintaining skin health, and their internal application in body and beauty is developing rapidly (e.g. monounsaturated and polyunsaturated fats, amino acids and vitamins E and C). Antioxidants, essential for skin health, can effectively reduce cellular oxidative stress.

[0004] Carotenoids are a widespread group of colorful phytochemicals that appeared early in life on Earth. Their main role is to absorb excess solar energy and mitigate the resulting oxidative stress (powerful antioxidants). The main carotenoids in the diet include beta-carotene, lycopene and lutein, all of which have a distinctive color, which limits their use in cosmetics. Carotenoids are essential for the body's first line of defense against environmental aggressors.

[0005] Phytoene and phytoene are colorless carotenoids, long overlooked due to their lack of color, present in all plants and are the precursors of all colored carotenoids, thus sharing the same properties (antioxidant, DNA protective, anti-inflammatory). Phytoene and phytoene absorb light in the UV range and participate in the natural photoprotection of the skin. Phytoene fluoresces green in the UV wavelengths, naturally enhances skin radiance, is less susceptible to oxidation, is better absorbed and selectively accumulates in the skin, resulting in better cosmetic results. Tomatoes are an excellent source of carotenoids, including phytoene and phytoene. Summary of the invention

[0006] In order to solve the above technical problems, the present invention provides a white tomato whitening and repairing composition, which comprises, by mass percentage, jojoba oil: 68-85%, squalane: 10-25%, and white tomato extract supplemented to 100%.

[0007] As an embodiment of the present invention, the composition comprises, by mass percentage: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

[0008] As an embodiment of the present invention, the preparation method of the white tomato extract is as follows:

[0009] Select freshly picked ripe white tomato fruits, wash and peel them, cut them into pieces, and add rosemary extract (1% w / v) into the pulp slurry;

[0010] Using a nanobubble generator, tiny oxygen bubbles were injected into a mixture of ethanol and olive extract (3:1 ratio); the treatment temperature was maintained at 25°C for 10 minutes;

[0011] The white tomato pulp was mixed with the nanobubble activated solvent in a ratio of 1:5; ultrasonic extraction was performed at 35°C with a frequency of 20 kHz for 30 minutes with intermittent operation (5 minutes on / 2 minutes off);

[0012] The organic phase and the aqueous phase were separated by a centrifuge (5000 rpm, 10 minutes), and the organic phase was collected; the extract was concentrated in a low-temperature vacuum evaporator, and the temperature was controlled below 35° C. to reduce the loss of volatile components;

[0013] The purified concentrate was freeze-dried and the obtained powder was placed in a brown bottle protected by inert gas (nitrogen) and stored at -20°C.

[0014] The invention provides a skin care product, comprising the white tomato whitening and repairing composition.

[0015] As an embodiment of the present invention, the raw materials for preparing the skin care product include:

[0016] 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1,3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant fermentation extract, and 0.5 parts of hexylene glycol.

[0017] As an embodiment of the present invention, the plant fermentation extract is a fermentation extract of white tea and oats.

[0018] As an embodiment of the present invention, the bacterial species used in the plant fermentation extract is Lactobacillus.

[0019] As an embodiment of the present invention, the steps of the method for preparing a skin care product are as follows:

[0020] (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved;

[0021] (2) Add arginine below 50 degrees and stir slowly;

[0022] (3) Add the remaining ingredients in turn and mix well.

[0023] As an embodiment of the present invention, the skin care product is a lotion or a cream.

[0024] As an embodiment of the present invention, the white tomato whitening and repairing composition is applied in the field of daily chemicals.

[0025] By adopting the above technical solution, the present invention has the following beneficial effects:

[0026] White tomatoes are rich in colorless carotenoids (such as phytoene and phytofluenza), which have excellent antioxidant and whitening abilities:

[0027] Inhibit melanin formation: Colorless carotenoids can inhibit tyrosinase activity, reduce the production of melanin, and whiten the skin from the root.

[0028] Prevents pigmentation: Antioxidant effect can reduce pigmentation caused by ultraviolet rays, prevent and reduce pigmentation. Even and bright skin tone: Continuous use can brighten the skin tone and make the skin look natural and radiant.

[0029] The carotenoids in white tomatoes can neutralize free radicals and reduce oxidative damage to skin cells caused by UV rays and pollution. The antioxidant activity can reduce the formation of wrinkles and fine lines, helping the skin maintain its elasticity and youthfulness.

[0030] White tomato extract can strengthen the skin barrier, promote cell metabolism, and improve the skin's self-repair ability. White tomato extract has anti-inflammatory properties and can effectively relieve redness and discomfort of sensitive skin. DETAILED DESCRIPTION

[0031] The technical solution of the present invention will be described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0032] The present invention is further explained below in conjunction with specific implementation modes.

[0033] Example 1

[0034] This embodiment provides a skin care product, the preparation raw materials of which include:

[0035] 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1,3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant fermentation extract, and 0.5 parts of hexylene glycol.

[0036] The repair composition comprises: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

[0037] The preparation method of the white tomato extract is as follows:

[0038] Select freshly picked ripe white tomato fruits, wash and peel them, cut them into pieces, and add rosemary extract (1% w / v) into the pulp slurry;

[0039] Using a nanobubble generator, tiny oxygen bubbles were injected into a mixture of ethanol and olive extract (3:1 ratio); the treatment temperature was maintained at 25°C for 10 minutes;

[0040] The white tomato pulp was mixed with the nanobubble activated solvent in a ratio of 1:5; ultrasonic extraction was performed at 35°C with a frequency of 20 kHz for 30 minutes with intermittent operation (5 minutes on / 2 minutes off);

[0041] The organic phase and the aqueous phase were separated by a centrifuge (5000 rpm, 10 minutes), and the organic phase was collected; the extract was concentrated in a low-temperature vacuum evaporator, and the temperature was controlled below 35° C. to reduce the loss of volatile components;

[0042] The purified concentrate was freeze-dried and the obtained powder was placed in a brown bottle protected by inert gas (nitrogen) and stored at -20°C.

[0043] Wherein, the preparation method of the rosemary extract is as follows:

[0044] Drying fresh rosemary leaves at a temperature below 60°C until the moisture content is less than 10%; crushing the dried leaves into coarse powder (particle size of about 0.5-1 mm);

[0045] Weigh 50g of rosemary powder, place it in a reflux extraction device, add 200mL of mixed solvent, and ensure that the sample is completely immersed; heat it to 60℃ in a constant temperature water bath, and reflux extract for 2 hours. After cooling, filter the extract with a Buchner funnel and filter paper.

[0046] The filtered extract was concentrated to a viscous liquid state under reduced pressure by a rotary evaporator at a temperature below 45°C. The concentrate was transferred to a separatory funnel and an appropriate amount of distilled water was added to separate the solution into two phases (polar phase and non-polar phase).

[0047] The extract was extracted with ethyl acetate three times, with an equal amount of ethyl acetate added each time (the ratio of the extraction solvent volume to the aqueous phase volume was about 1:1). The ethyl acetate phase was washed with a small amount of distilled water to remove impurities until the pH of the washing solution was close to neutral. A small amount of anhydrous sodium sulfate was added to the ethyl acetate phase and allowed to stand for 12 hours to remove moisture. After filtration, the filtrate was again passed through a rotary evaporator to remove ethyl acetate to obtain a rosemary extract.

[0048] The preparation method of the olive extract is as follows:

[0049] Ripe olive fruits are used, and the fruits are dried at a temperature below 40°C; the fruits are crushed into coarse powder with a particle size of about 0.5-1 mm.

[0050] Add 50 g of powder to a reflux device, add 300 mL of hexane, and reflux extract in a 60°C constant temperature water bath for 2 hours. After cooling, filter with a Buchner funnel to collect the extract.

[0051] The extract was concentrated under reduced pressure at 40-50°C by a rotary evaporator to remove the solvent to obtain an oily concentrate. The concentrate was transferred to a separatory funnel and distilled water (the ratio of solvent to water was about 1:1) was added. The oily component was extracted with ethyl acetate and repeated 3 times; the ethyl acetate phase was collected; the olive extract was obtained after washing and drying.

[0052] The plant fermentation extract is a fermentation extract of white tea and oats, and the specific preparation method is as follows:

[0053] 1. Soak 10g of white tea leaves in 250mL of 80℃ distilled water for 30 minutes and stir evenly. Cool to room temperature and filter. Take the tea liquid for later use.

[0054] 2. Oat processing: Grind oat granules into powder. Add 10g oat powder to 250mL distilled water and stir evenly. Heat to 60℃, maintain for 10 minutes, cool to room temperature, filter, and take the filtrate for later use.

[0055] 3. Mix the white tea liquid and oatmeal liquid in a volume ratio of 1:1 (total volume 500mL). Adjust the mixture to pH 6.5 and dispense the mixture into sterile triangular bottles (the volume of each bottle should not exceed 2 / 3 of the total volume) at 121℃ for 15 minutes to ensure aseptic operation. Cool to 37℃ and set aside.

[0056] 3. Bacteria activation: Lactiplantibacillus The lyophilized powder was added to 10 mL of culture medium (casein peptone (trypsin digest) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, agar 15.0 g, distilled water 1000.0 mL), and activated at 37°C for 12 hours. The activated bacterial solution was added to the cooled mixed solution, and the inoculum amount was 6% (v / v).

[0057] Temperature: 37°C. Time: 36 hours. Maintain low stirring speed of 100 rpm.

[0058] After fermentation, the mixture was centrifuged at 5000 rpm for 10 minutes to separate the precipitate and the supernatant. The supernatant was filtered with a 0.22 μm filter membrane to obtain a clear fermentation broth. The fermentation broth was further treated by low-temperature pasteurization (65°C, 30 minutes) to extend the shelf life.

[0059] The steps of the preparation method of the skin care product are as follows:

[0060] (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved;

[0061] (2) Add arginine below 50 degrees and stir slowly;

[0062] (3) Add the remaining ingredients in turn and mix well.

[0063] Example 2

[0064] This embodiment provides a skin care product, the preparation raw materials of which include:

[0065] 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1,3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant fermentation extract, and 0.5 parts of hexylene glycol.

[0066] The repair composition comprises: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

[0067] The preparation method of the white tomato extract is as follows:

[0068] Select freshly picked ripe white tomatoes, wash and peel them, cut them into pieces, and add 0.01% butylated hydroxytoluene to the pulp slurry;

[0069] Using a nanobubble generator, tiny oxygen bubbles were injected into a mixture of ethanol and olive extract (3:1 ratio); the treatment temperature was maintained at 25°C for 10 minutes;

[0070] The white tomato pulp was mixed with the nanobubble activated solvent in a ratio of 1:5; ultrasonic extraction was performed at 35°C with a frequency of 20 kHz for 30 minutes with intermittent operation (5 minutes on / 2 minutes off);

[0071] The organic phase and the aqueous phase were separated by a centrifuge (5000 rpm, 10 minutes), and the organic phase was collected; the extract was concentrated in a low-temperature vacuum evaporator, and the temperature was controlled below 35° C. to reduce the loss of volatile components;

[0072] The purified concentrate was freeze-dried and the obtained powder was placed in a brown bottle protected by inert gas (nitrogen) and stored at -20°C.

[0073] The preparation method of the olive extract is as follows:

[0074] Ripe olive fruits are used, and the fruits are dried at a temperature below 40°C; the fruits are crushed into coarse powder with a particle size of about 0.5-1 mm.

[0075] Add 50 g of powder to a reflux device, add 300 mL of hexane, and reflux extract in a 60°C constant temperature water bath for 2 hours. After cooling, filter with a Buchner funnel to collect the extract.

[0076] The extract was concentrated under reduced pressure at 40-50°C by a rotary evaporator to remove the solvent to obtain an oily concentrate. The concentrate was transferred to a separatory funnel and distilled water (the ratio of solvent to water was about 1:1) was added. The oily component was extracted with ethyl acetate and repeated 3 times; the ethyl acetate phase was collected; the olive extract was obtained after washing and drying.

[0077] The plant fermentation extract is a fermentation extract of white tea and oats, and the specific preparation method is as follows:

[0078] 1. Soak 10g of white tea leaves in 250mL of 80℃ distilled water for 30 minutes and stir evenly. Cool to room temperature and filter. Take the tea liquid for later use.

[0079] 2. Oat processing: Grind oat granules into powder. Add 10g oat powder to 250mL distilled water and stir evenly. Heat to 60℃, maintain for 10 minutes, cool to room temperature, filter, and take the filtrate for later use.

[0080] 3. Mix the white tea liquid and oatmeal liquid in a volume ratio of 1:1 (total volume 500mL). Adjust the mixture to pH 6.5 and dispense the mixture into sterile triangular bottles (the volume of each bottle should not exceed 2 / 3 of the total volume) at 121℃ for 15 minutes to ensure aseptic operation. Cool to 37℃ and set aside.

[0081] 3. Bacteria activation: Lactiplantibacillus The lyophilized powder was added to 10 mL of culture medium (casein peptone (trypsin digest) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, agar 15.0 g, distilled water 1000.0 mL), and activated at 37°C for 12 hours. The activated bacterial solution was added to the cooled mixed solution, and the inoculum amount was 6% (v / v).

[0082] Temperature: 37°C. Time: 36 hours. Maintain low stirring speed of 100 rpm.

[0083] After fermentation, the mixture was centrifuged at 5000 rpm for 10 minutes to separate the precipitate and the supernatant. The supernatant was filtered with a 0.22 μm filter membrane to obtain a clear fermentation broth. The fermentation broth was further treated by low-temperature pasteurization (65°C, 30 minutes) to extend the shelf life.

[0084] The steps of the preparation method of the skin care product are as follows:

[0085] (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved;

[0086] (2) Add arginine below 50 degrees and stir slowly;

[0087] (3) Add the remaining ingredients in turn and mix well.

[0088] Example 3

[0089] This embodiment provides a skin care product, the preparation raw materials of which include:

[0090] 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1,3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant fermentation extract, and 0.5 parts of hexylene glycol.

[0091] The repair composition comprises: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

[0092] The preparation method of the white tomato extract is as follows:

[0093] Take fresh white tomatoes, remove the skin, stems and seeds, and keep only the flesh. Wash with cold distilled water, cut into pieces and crush into a uniform pulp.

[0094] Place the white tomato pulp in an extraction container and add an antioxidant (BHT, 0.01%). Use an ultrasonic crusher (power 200-300W, 20 minutes)

[0095] Add mixed solvent (n-hexane: acetone: ethanol = 3:1:1) in a ratio of 1:4 to the sample weight. Stir gently in a 30°C water bath for 30 minutes. Centrifuge the extract at 5000 rpm for 15 minutes and take the supernatant.

[0096] The extract was concentrated in a rotary evaporator to remove most of the solvent and the temperature was controlled at 35-40°C.

[0097] The plant fermentation extract is a fermentation extract of white tea and oats, and the specific preparation method is as follows:

[0098] 1. Soak 10g of white tea leaves in 250mL of 80℃ distilled water for 30 minutes and stir evenly. Cool to room temperature and filter. Take the tea liquid for later use.

[0099] 2. Oat processing: Grind oat granules into powder. Add 10g oat powder to 250mL distilled water and stir evenly. Heat to 60℃, maintain for 10 minutes, cool to room temperature, filter, and take the filtrate for later use.

[0100] 3. Mix the white tea liquid and oatmeal liquid in a volume ratio of 1:1 (total volume 500mL). Adjust the mixture to pH 6.5 and dispense the mixture into sterile triangular bottles (the volume of each bottle should not exceed 2 / 3 of the total volume) at 121℃ for 15 minutes to ensure aseptic operation. Cool to 37℃ and set aside.

[0101] 3. Bacteria activation: Lactiplantibacillus The lyophilized powder was added to 10 mL of culture medium (casein peptone (trypsin digest) 10.0 g, beef extract powder 10.0 g, yeast extract powder 5.0 g, glucose 20.0 g, Tween 80 1.0 g, sodium acetate 5.0 g, triammonium citrate 2.0 g, K2HPO4 2.0 g, MgSO4·7H2O 0.2 g, MnSO4·H2O 0.05 g, agar 15.0 g, distilled water 1000.0 mL), and activated at 37°C for 12 hours. The activated bacterial solution was added to the cooled mixed solution, and the inoculum amount was 6% (v / v).

[0102] Temperature: 37°C. Time: 36 hours. Maintain low stirring speed of 100 rpm.

[0103] After fermentation, the mixture was centrifuged at 5000 rpm for 10 minutes to separate the precipitate and the supernatant. The supernatant was filtered with a 0.22 μm filter membrane to obtain a clear fermentation broth. The fermentation broth was further treated by low-temperature pasteurization (65°C, 30 minutes) to extend the shelf life.

[0104] The steps of the preparation method of the skin care product are as follows:

[0105] (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved;

[0106] (2) Add arginine below 50 degrees and stir slowly;

[0107] (3) Add the remaining ingredients in turn and mix well.

[0108] Example 4

[0109] This embodiment provides a skin care product, the preparation raw materials of which include:

[0110] 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1,3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant extract, and 0.5 parts of hexylene glycol.

[0111] The repair composition comprises: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

[0112] The preparation method of the white tomato extract is as follows:

[0113] Select freshly picked ripe white tomato fruits, wash and peel them, cut them into pieces, and add rosemary extract (1% w / v) into the pulp slurry;

[0114] Using a nanobubble generator, tiny oxygen bubbles were injected into a mixture of ethanol and olive extract (3:1 ratio); the treatment temperature was maintained at 25°C for 10 minutes;

[0115] The white tomato pulp was mixed with the nanobubble activated solvent in a ratio of 1:5; ultrasonic extraction was performed at 35°C with a frequency of 20 kHz for 30 minutes with intermittent operation (5 minutes on / 2 minutes off);

[0116] The organic phase and the aqueous phase were separated by a centrifuge (5000 rpm, 10 minutes), and the organic phase was collected; the extract was concentrated in a low-temperature vacuum evaporator, and the temperature was controlled below 35° C. to reduce the loss of volatile components;

[0117] The purified concentrate was freeze-dried and the obtained powder was placed in a brown bottle protected by inert gas (nitrogen) and stored at -20°C.

[0118] Wherein, the preparation method of the rosemary extract is as follows:

[0119] Drying fresh rosemary leaves at a temperature below 60°C until the moisture content is less than 10%; crushing the dried leaves into coarse powder (particle size of about 0.5-1 mm);

[0120] Weigh 50g of rosemary powder, place it in a reflux extraction device, add 200mL of mixed solvent, and ensure that the sample is completely immersed; heat it to 60℃ in a constant temperature water bath, and reflux extract for 2 hours. After cooling, filter the extract with a Buchner funnel and filter paper.

[0121] The filtered extract was concentrated to a viscous liquid state under reduced pressure by a rotary evaporator at a temperature below 45°C. The concentrate was transferred to a separatory funnel and an appropriate amount of distilled water was added to separate the solution into two phases (polar phase and non-polar phase).

[0122] The extract was extracted with ethyl acetate three times, with an equal amount of ethyl acetate added each time (the ratio of the extraction solvent volume to the aqueous phase volume was about 1:1). The ethyl acetate phase was washed with a small amount of distilled water to remove impurities until the pH of the washing solution was close to neutral. A small amount of anhydrous sodium sulfate was added to the ethyl acetate phase and allowed to stand for 12 hours to remove moisture. After filtration, the filtrate was again passed through a rotary evaporator to remove ethyl acetate to obtain a rosemary extract.

[0123] The preparation method of the olive extract is as follows:

[0124] Ripe olive fruits are used, and the fruits are dried at a temperature below 40°C; the fruits are crushed into coarse powder with a particle size of about 0.5-1 mm.

[0125] Add 50 g of powder to a reflux device, add 300 mL of hexane, and reflux extract in a 60°C constant temperature water bath for 2 hours. After cooling, filter with a Buchner funnel to collect the extract.

[0126] The extract was concentrated under reduced pressure at 40-50°C by a rotary evaporator to remove the solvent to obtain an oily concentrate. The concentrate was transferred to a separatory funnel and distilled water (the ratio of solvent to water was about 1:1) was added. The oily component was extracted with ethyl acetate and repeated 3 times; the ethyl acetate phase was collected; the olive extract was obtained after washing and drying.

[0127] The plant extract is a mixed water extract of white tea and oats, and the specific preparation method is as follows:

[0128] 1. Soak 10g of white tea leaves in 250mL of 80℃ distilled water for 30 minutes and stir evenly. Cool to room temperature and filter. Take the tea liquid for later use.

[0129] 2. Oat processing: Grind oat granules into powder. Add 10g oat powder to 250mL distilled water and stir evenly. Heat to 60℃, maintain for 10 minutes, cool to room temperature, filter, and take the filtrate for later use.

[0130] 3. Mix the tea liquid and oatmeal filtrate in a 1:1 ratio.

[0131] The steps of the preparation method of the skin care product are as follows:

[0132] (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved;

[0133] (2) Add arginine below 50 degrees and stir slowly;

[0134] (3) Add the remaining ingredients in turn and mix well.

[0135] Example 5

[0136] This embodiment provides a skin care product, the preparation raw materials of which include:

[0137] 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1,3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant fermentation extract, and 0.5 parts of hexylene glycol.

[0138] The repair composition comprises: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

[0139] The preparation method of the white tomato extract is as follows:

[0140] Take fresh white tomatoes, remove the skin, stems and seeds, and keep only the flesh. Wash with cold distilled water, cut into pieces and crush into a uniform pulp.

[0141] Place the white tomato pulp in an extraction container and add an antioxidant (BHT, 0.01%). Use an ultrasonic crusher (power 200-300W, 20 minutes)

[0142] Add mixed solvent (n-hexane: acetone: ethanol = 3:1:1) in a ratio of 1:4 to the sample weight. Stir gently in a 30°C water bath for 30 minutes. Centrifuge the extract at 5000 rpm for 15 minutes and take the supernatant.

[0143] The extract was concentrated in a rotary evaporator to remove most of the solvent and the temperature was controlled at 35-40°C.

[0144] The plant fermentation extract is a fermentation extract of white tea and oats, and the specific preparation method is as follows:

[0145] 1. Soak 10g of white tea leaves in 250mL of 80℃ distilled water for 30 minutes and stir evenly. Cool to room temperature and filter. Take the tea liquid for later use.

[0146] 2. Oat processing: Grind oat granules into powder. Add 10g oat powder to 250mL distilled water and stir evenly. Heat to 60℃, maintain for 10 minutes, cool to room temperature, filter, and take the filtrate for later use.

[0147] 3. Mix the tea liquid and oatmeal filtrate in a 1:1 ratio.

[0148] The steps of the preparation method of the skin care product are as follows:

[0149] (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved;

[0150] (2) Add arginine below 50 degrees and stir slowly;

[0151] (4) Add the remaining ingredients in turn and mix well.

[0152] Performance Testing

[0153] Test 1: The skin care products prepared in Examples 1 to 5 were placed at 0°C, 25°C, and 40°C for 90 days to check the properties and color of the products. The test results are shown in Table 1.

[0154] Hot and cold cycle experiment: Place the emulsions prepared in Examples 1 to 6 into a sealed container to ensure that it is completely sealed to prevent interference from external humidity or gas. Place the sample in a thermostat set at 45°C for 12 hours. After taking out the sample, quickly transfer it to a low-temperature box at -5°C and keep it for 12 hours. After completing one high and low temperature cycle, repeat 6 times; after the cycle, check the physical properties of the sample such as appearance, color, odor, viscosity, stratification, and particle precipitation.

[0155] Table 1 Test results of performance test 1

[0156] Example 0℃ 25℃ 40℃ Hot and cold cycle experiment Example 1 No change No change No change No change Example 2 No change No change Slightly delaminated Slightly delaminated Example 3 No change No change Slightly delaminated Slightly delaminated Example 4 No change Slightly delaminated Slightly delaminated Slightly delaminated Example 5 Slightly delaminated Slightly delaminated Slightly delaminated Slightly delaminated

[0157] Test 2:

[0158] Tyrosinase inhibition test:

[0159] Levodopa was used as substrate (1 mg / mL) and kojic acid as positive control. The 96-well plate was used as carrier and the experiment was divided into 4 groups: reaction group, reaction control group, blank group, and blank control group. 25 μL samples of different concentrations were added to the reaction group and reaction control group, and 25 μL phosphate buffer was added to the blank group and blank control group. Subsequently, 25 μL tyrosinase was added to the reaction group and blank group, while the reaction control group and blank control group replaced tyrosinase with an equal amount of PBS. The mixture was mixed evenly, and 100 μL of levodopa solution was added to each well after incubation at 37°C for 5 minutes. After continuing to react in a 37°C incubator for 5 minutes, the absorbance (A) value was measured at a wavelength of 475 nm. The experimental data were all averaged after 3 repetitions. The tyrosinase inhibition rate was calculated according to the following formula, and the IC50 value was calculated using SPSS software.

[0160] Table 2 Test results of performance test 2 (n=3)

[0161] Example Group <![CDATA[IC 50 / (μmol·L -1 )]]> Example 1 0.29±0.24*** Example 2 0.76±0.11** Example 3 1.83±0.30** Example 4 1.35±0.16** Example 5 2.56±0.27** Kojic Acid 0.81±0.37

[0162] Test 3:

[0163] Hyaluronidase inhibition assay

[0164] The test is based on the HMC-W1-029 hyaluronidase inhibition rate.

[0165] Example 1-5: Dilute with pure water to a sample concentration of 0.2%;

[0166] Positive control (dipotassium glycyrrhizinate, purity ≥98%): diluted with water to a positive control concentration of 3%;

[0167] Negative control: pure water.

[0168] Test operation steps:

[0169] Design the embodiment group, positive control group and negative control group, and set up 3 parallels for each group. Add different reagent solutions respectively, shake well, and place at room temperature for 30 minutes for color development. Use ultraviolet spectrophotometer to measure the absorbance value at a wavelength of 528nm.

[0170] The statistical analysis software was SPSS, and the comparison of hyaluronidase inhibition rates among the test samples, positive controls, and negative controls was performed using an independent sample t test. The above statistical analyses were all two-tailed tests, with a significance level of α = 0.05, P > 0.05, indicating no significant difference between the two groups; P < 0.05, indicating a significant difference between the two groups.

[0171] Table 3 Test results

[0172] name Experimental Results P-value Example 1 62.251±0.215 ＜0.05 Example 2 59.379±0.228 ＜0.05 Example 3 51.251±0.251 ＜0.05 Example 4 56.147±0.384 ＜0.05 Example 5 49.381±0.417 ＜0.05 Negative control -4.168±0.381 / Positive Control 50.841±3.413 ＜0.05

[0173] The hyaluronidase inhibition rate was rounded to three decimal places, and P < 0.05 indicated that there were significant differences between the sample and the positive control and the negative control.

[0174] Test 4: Transepidermal Water Loss Rate Difference

[0175] Healthy subjects aged 25-60 years old (all subjects already have sensitive skin characteristics, such as allergies, redness, etc.), including 8 males and 22 females, with an average age of 42.53 years old.

[0176] Part used: hand and forearm

[0177] Usage: When used on the forearm, the test sample is (2.0±0.1) mg / cm 2 Apply a single application of the dosage and use a latex finger cot to evenly spread the sample in the test area.

[0178] Test steps

[0179] The subjects did not touch the inner part of their arms with water before the visit.

[0180] Before the experiment, the subjects were required to uniformly clean their arms with dry facial tissue;

[0181] Two measurement areas were marked on the inner side of the left or right arm of the subject, each area was 3cm*3cm, and the distance between the areas was at least 1cm; the sample area and the negative control area were randomly distributed in the calibration area to ensure that the position of each area was statistically balanced: the subject exposed his arm and rested for 30 minutes in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH; the inducer (0.1% histamine solution) was applied to the sample area and the negative area for 10 minutes, and after the application and before the use of the sample (Timm), the transepidermal water loss rate of the calibration area was tested with Tewameter, and the hemoglobin of the calibration area was tested with Mexamter; the sample was used in the calibrated sample area according to the sample use method; after the sample was used, the subject exposed his arm in an environment with a temperature of 21±1℃ and a relative humidity of 50±10%RH and rested for 1 hour (T1), and the transepidermal water loss rate of the calibration area was tested with Tewameter; 1 hour later (T1), the hemoglobin of the calibration area was tested with Mexamter.

[0182] Transepidermal water loss rate difference

[0183] Courage+Khazaka Transepidermal Water Loss and Evaporative Heat Loss Test Probe Tewameter TM300 is used to measure transepidermal water loss.

[0184] Table 4 Comparison of transepidermal water loss rate before and after

[0185] Example Same as before (mean ± standard deviation) After 1 hour of use (mean ± standard deviation) Example 1 13.15±1.43 9.01±2.56 Example 2 13.26±3.01 9.92±2.81 Example 3 13.30±2.13 11.83±2.18 Example 4 13.28±2.06 10.82±2.72 Example 5 13.32±1.78 12.14±2.91 Negative area 13.22±2.14 12.73±3.01

[0186] Heme difference.

[0187] Courage+Khazaka Skin Melanin and Hemoglobin Tester Mexamter MX18 is used to detect skin hemoglobin. The hemoglobin difference refers to the hemoglobin in the calibration area before and 1 hour after sampling.

[0188] Table 5 Comparison of hemoglobin before and after

[0189]

[0190]

[0191] The plant extract of the present invention replaces the chemical antioxidant, which can not only protect the target compound from being oxidized, but also use nano-enhancement technology to increase the activity of the solvent and improve the dissolution efficiency. At the same time, due to the stability and water solubility requirements of the white tomato extract in the formula, it is easy to produce stratification, flocculation and other phenomena in the emulsion formula. The present invention increases the fermented plant extract and plant antioxidant in the formula, and combines the emulsifier to improve the stability.

[0192] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A white tomato whitening and repairing composition, characterized in that: According to mass percentage, the composition comprises: jojoba oil: 68-85%, squalane: 10-25%, and white tomato extract supplemented to 100%.

2. A white tomato whitening and repairing composition according to claim 1, characterized in that: According to mass percentage, the composition comprises: jojoba oil: 70%, squalane: 15%, and white tomato extract supplemented to 100%.

3. A white tomato whitening and repairing composition according to claim 1, characterized in that: The preparation method of the white tomato extract is as follows: Select freshly picked ripe white tomato fruits, wash and peel them, cut them into pieces, and add rosemary extract (1% w / v) into the pulp slurry; Using a nanobubble generator, tiny oxygen bubbles were injected into a mixture of ethanol and olive extract (3:1 ratio); the treatment temperature was maintained at 25°C for 10 minutes; The white tomato pulp was mixed with the nanobubble activated solvent in a ratio of 1:5; ultrasonic extraction was performed at 35°C with a frequency of 20 kHz for 30 minutes with intermittent operation (5 minutes on / 2 minutes off); The organic phase and the aqueous phase were separated by a centrifuge (5000 rpm, 10 minutes), and the organic phase was collected; the extract was concentrated in a low-temperature vacuum evaporator, and the temperature was controlled below 35° C. to reduce the loss of volatile components; The purified concentrate was freeze-dried and the obtained powder was placed in a brown bottle protected by inert gas (nitrogen) and stored at -20°C.

4. A skin care product, characterized in that: A white tomato whitening and repairing composition comprising any one of claims 1 to 3.

5. A skin care product according to claim 4, characterized in that: The raw materials for its preparation include: 91.88 parts of deionized water, 0.1 parts of Carbopol 941, 4 parts of 1.3-butylene glycol, 0.1 parts of allantoin, 0.1 parts of sodium hyaluronate, 0.25 parts of p-hydroxyacetophenone, 0.02 parts of EDTA-2Na, 2 parts of isononyl isononanoate, 0.5 parts of M68 emulsifier, 0.1 parts of arginine, 0.15 parts of repair composition, 0.3 parts of plant fermentation extract, and 0.5 parts of hexylene glycol.

6. A skin care product according to claim 5, characterized in that: The plant fermentation extract is a fermentation extract of white tea and oats.

7. A skin care product according to claim 5, characterized in that: The bacterial species used in the plant fermentation extract is lactobacillus.

8. The method for preparing a skin care product according to claim 5, characterized in that: The steps are as follows: (1) Heat deionized water, Carbopol 941, 1,3-butylene glycol, allantoin, sodium hyaluronate, p-hydroxyacetophenone, EDTA-2Na, isononyl isononanoate, and M68 emulsifier to 85 degrees, homogenize for 2-3 minutes, and stir evenly until completely dissolved; (2) Add arginine below 50 degrees and stir slowly; (3) Add the remaining ingredients in turn and mix well.

9. A skin care product according to claim 5, characterized in that: The skin care products are lotions and creams.

10. The white tomato whitening and repairing composition according to claim 1, characterized in that: It is used in the field of daily chemicals.

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