Wearable circulating tumor cell capturing device as well as use method and application thereof

Abstract

The invention relates to the technical field of medical instruments, in particular to a wearable circulating tumor cell capturing device and a using method and application thereof, and the wearable circulating tumor cell capturing device comprises a magnetic chip, a magnetic bead dispenser and a controller; a connecting pipe is arranged between the magnetic bead dispenser and the magnetic chip, and the output end of the magnetic bead dispenser is communicated with the input end of the magnetic chip through the connecting pipe; the output end of the magnetic chip communicates with a pump assembly, the output end of the pump assembly communicates with a first venous catheter, the end, away from the pump assembly, of the first venous catheter communicates with the wearable body, and the end, away from the first venous catheter, of the wearable body communicates with a second venous catheter. One end, far away from the wearing main body, of the second venous catheter is communicated with the connecting pipe and the output end of the magnetic bead dispenser; the connecting pipe is sleeved with a magnetron of a rectangular structure, and a magnetic bead control assembly used for conducting track control on magnetic beads passing through the connecting pipe is arranged in the magnetron. According to the invention, the distribution of the magnetic beads in the connecting pipe can be accurately controlled, and the capturing efficiency of CTCs is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a wearable circulating tumor cell capture device and a use method and application thereof. Background Art

[0002] CTCs refer to tumor cells that originate from primary or metastatic tumors and fall off into the peripheral blood circulation. Because they carry relevant biological information of primary or metastatic lesions and can spread to other tissues and organs throughout the body through the blood circulation, CTCs are considered a promising non-invasive cancer diagnostic marker. CTCs can be captured today through a variety of technologies, among which a commonly used technology is to use an intravenous indwelling needle combined with a specific CTCs capture device. This technology allows the indwelling needle to remain stationary in the vein for 30 minutes, and uses epithelial cell adhesion protein antibodies bound to the surface of the functional area to specifically capture CTCs in vivo. This technology is limited in convenience due to the long retention time of the indwelling needle in the collection subject and the large number of collections.

[0003] Compared with traditional venipuncture sampling, the wearable circulating tumor cell capture device can continuously and long-term screen the blood in the patient's vein, thereby greatly improving the capture efficiency of CTCs. The device uses the specific binding ability of magnetic beads and magnetic chips to efficiently capture CTCs, providing a rich sample basis for further research or prognosis analysis of tumors.

[0004] In the prior art, the wearable circulating tumor cell capture device flows out of the magnetic bead storage chamber through the magnetic beads and enters the injection device, and the blood in the wearer's body flows into the double-lumen venous catheter and is stored in the injection device; with the help of external force, the magnetic beads and the blood flowing out of the double-lumen venous catheter are pumped into the magnetic chip for mixing, and the magnetic beads capture the circulating tumor cells and are retained in the chip under the action of magnetic force, and the processed blood can return to the human body through the double-lumen venous catheter, thereby realizing the circulation capture of blood in the body. Although this technology can be wearable and use magnetic beads to capture CTCs, since the magnetic beads are arranged in the tube through the injection device, the blood circulation is used to drive the magnetic beads to capture CTCs in the tube and move to the magnetic chip for collection, but because the wearer is in motion or the tube is displaced, bent or deformed, the magnetic beads may gather or move irregularly at a certain position in the tube under the action of gravity and centrifugal force, thereby greatly affecting the efficiency of magnetic beads in capturing CTCs. At the same time, the long capture time also affects the wearing comfort of the wearer.

[0005] In summary, how to solve the problem that when the wearable body moves or the tube is displaced, bent or deformed, the magnetic beads may aggregate or move irregularly at a certain position in the tube under the action of gravity and centrifugal force, thereby greatly affecting the efficiency of magnetic beads in capturing CTCs has become a difficult problem that needs to be urgently solved in this field. Therefore, it is necessary to propose a wearable circulating tumor cell capture device and its use method and application. Summary of the invention

[0006] To solve the above problems, the present invention provides a wearable circulating tumor cell capture device and a method of using and application thereof. By utilizing the magnetic beads in the connecting tube, driven by the blood circulation, and then under the magnetic action between the magnetism of the magnetic beads and the first electromagnet, the magnetic beads can be distributed along the magnetic flux lines between adjacent horizontally symmetrical first electromagnets, thereby achieving precise control of the distribution of the magnetic beads in the connecting tube, so that the magnetic beads can be evenly distributed in the connecting tube, avoiding aggregation between the magnetic beads, and improving the capture efficiency.

[0007] In order to achieve the above-mentioned purpose, the technical solution of the present invention is as follows: a wearable circulating tumor cell capture device and its use method and application, including a magnetic chip, a magnetic bead dispenser and a controller; a connecting tube is provided between the magnetic bead dispenser and the magnetic chip, and the output end of the magnetic bead dispenser and the input end of the magnetic chip are unidirectionally connected through the connecting tube.

[0008] The output end of the magnetic chip is connected to a pump assembly, and a controller is used to control the operation of the pump assembly; the output end of the pump assembly is connected to a first venous catheter, an end of the first venous catheter away from the pump assembly is connected to a wearable body, an end of the wearable body away from the first venous catheter is connected to a second venous catheter, an end of the second venous catheter away from the wearable body is unidirectionally connected to a position where the connecting tube and the output end of the magnetic bead dispenser are connected, and the controller is used to control the magnetic bead dispenser to dispense magnetic beads.

[0009] A magnetron in a rectangular structure is connected to the outer surface of the connecting tube, and a magnetic bead control component for controlling the trajectory of magnetic beads passing through the connecting tube is arranged inside the magnetron.

[0010] The technical principles of the above scheme are as follows:

[0011] The device uses a magnetic bead dispenser to release magnetic beads with specific antibodies. The magnetic beads circulate in the blood and bind to CTCs. When passing through a magnetic chip, the magnetic field is used to adsorb the magnetic beads bound to CTCs onto the chip surface, thereby capturing CTCs. The pump component drives the mixture of blood and magnetic beads to circulate in the device, while the magnetic bead control component in the magnetron precisely controls the distribution of the magnetic beads to ensure capture efficiency.

[0012] The above scheme has the following beneficial effects:

[0013] 1. The present invention uses a magnetic bead control component in a magnetron and utilizes the principle that the magnetic flux lines of electromagnets with the same pole and electromagnets with different poles are distributed differently. At the same time, the magnetic beads are small and can be accurately distributed on the magnetic flux lines, thereby achieving accurate control of the distribution of the magnetic beads in the connecting tube, ensuring that the magnetic beads can be evenly distributed during the blood circulation process and avoiding the aggregation of the magnetic beads. Since the magnetic beads can evenly and effectively bind to CTCs in the circulation, the capture accuracy and efficiency of CTCs are significantly improved.

[0014] 2. In actual applications, the wearable subject may perform various movements or posture changes, which may cause the connecting tube to bend or deform. The present invention can adapt to these changes well through the design of the magnetron and the magnetic bead control component. Even if the connecting tube is bent or deformed, the magnetic bead control component can ensure the uniform distribution of the magnetic beads in the connecting tube. This design not only improves the adaptability of the device, but also ensures that the capture efficiency can be kept stable under different conditions. In addition, due to the integrated design of the magnetron and the magnetic bead control component, the influence of external interference on the performance of the device is reduced, thereby improving the reliability of the device.

[0015] 3. The present invention does not rely on external forces, such as syringes and other tools, to enable the blood and magnetic beads to circulate, but relies on the power provided by the pump assembly to achieve natural and stable circulation of blood and magnetic beads in the device, which not only avoids the inconvenience and safety hazards that may be caused by external force operation, but also further reduces the impact of uneven external force on the wearer's blood circulation system; at the same time, the precise control of the pump assembly also ensures the uniform flow of blood and magnetic beads in the device, thereby improving the capture efficiency of CTCs; the design of the device also takes into account the comfort of the wearer, and through reasonable layout and material selection, the device can maintain good comfort and stability when worn for a long time.

[0016] Furthermore, the magnetic bead control component includes a magnetron, which is sleeved on the connecting tube. The magnetron has first grooves horizontally symmetrically opened along its length direction. Several first electromagnets are fixedly connected in the first grooves. The controller is used to control the magnetic pole reversal of the first electromagnets.

[0017] Beneficial effect: by controlling the magnetic pole reversal of the first electromagnet through the controller, the magnetic flux lines between adjacent horizontally symmetrical first electromagnets will change; at the same time, the magnetic beads in the connecting tube, driven by blood circulation, and then due to the magnetic effect between the magnetism of the magnetic beads and the first electromagnets, can be distributed along the magnetic flux lines between adjacent horizontally symmetrical first electromagnets, so that the distribution of the magnetic beads in the connecting tube can be accurately controlled, so that the magnetic beads can be evenly distributed in the connecting tube, avoiding aggregation between the magnetic beads, and improving the capture efficiency.

[0018] Furthermore, a second groove is opened in the magnetron along its length direction, and a plurality of second electromagnets are fixedly connected in the second groove. The controller is used to control the opening and closing of the second electromagnets.

[0019] Beneficial effects: The introduction of the second electromagnet provides an additional means of magnetic field control. The controller can control the opening and closing of the second electromagnet in sequence, so that the magnetic beads can move along the second electromagnet in sequence, thereby achieving controllable movement trajectory of the magnetic beads. At the same time, a part of the magnetic beads can be transported to the magnetic chip through the second electromagnet first, and the capture effect of this part of the magnetic beads on CTCs can be detected, thereby providing feedback on the control of the first electromagnet on the distribution of the magnetic beads.

[0020] Furthermore, a first one-way valve is connected in the first venous catheter, and a first filter is connected between the first one-way valve in the first venous catheter and the wearable body.

[0021] Beneficial effects: The setting of the first one-way valve ensures that blood can only flow in one direction, preventing the reduction of capture efficiency and possible infection risks caused by blood reflux; it ensures the unidirectionality of blood flow from the wearable body to the magnetic chip, and improves the stability and safety of the system.

[0022] Furthermore, when the magnetic beads flow through the magnetron, the controller controls the current supplied to the first electromagnet to adjust the distribution of the magnetic beads in the connecting tube, including:

[0023] The controller controls the current directions of the horizontally symmetrical and adjacent first electromagnets to be the same, so that the opposite ends of the two first electromagnets are the same magnetic poles, so that the magnetic flux lines between the first electromagnets are diffusely distributed, and the magnetic beads are diffusely distributed in the connecting tube along the magnetic flux lines between the first electromagnets; the controller controls the current directions of the horizontally symmetrical and adjacent first electromagnets to be opposite, so that the opposite ends of the two first electromagnets are opposite magnetic poles, so that the magnetic flux lines between the first electromagnets are converged, and the magnetic beads are linearly converged in the connecting tube along the magnetic flux lines between the first electromagnets.

[0024] Beneficial effects: By precisely controlling the magnetic pole reversal of the first electromagnet, the distribution state of the magnetic beads in the connecting tube can be dynamically adjusted, thereby preventing the magnetic beads from gathering or moving irregularly at a certain position in the tube due to gravity or centrifugal force. This dynamic adjustment ensures the uniform distribution of the magnetic beads during blood circulation and further improves the capture efficiency of CTCs. In addition, by adjusting the current direction and intensity of the electromagnet, the speed and direction of the movement of the magnetic beads can be fine-tuned, thereby optimizing the binding process of the magnetic beads and CTCs and improving the capture effect.

[0025] Furthermore, the second venous catheter is connected to a second one-way valve.

[0026] Beneficial effects: The second one-way valve also ensures that blood can only flow in one direction, from the magnetic chip back to the wearable body, which helps maintain the blood flow balance of the system and prevents potential problems caused by backflow.

[0027] Furthermore, a second filter is connected between the second one-way valve and the connecting pipe in the second venous catheter.

[0028] Beneficial effects: The second filter further purifies the blood after being treated by the magnetic chip, removes any remaining magnetic beads or other impurities, helps protect the wearer's vascular system, and reduces unnecessary stimulation and potential risks.

[0029] Furthermore, the second venous catheter, the output end of the magnetic bead dispenser and the connecting tube are interconnected through a tee.

[0030] Beneficial effects: The three-way setting simplifies the connection of the pipeline, making the circulation of blood and magnetic beads smoother, avoiding complex pipeline layout and possible leakage risks, and improving the reliability and ease of use of the system.

[0031] Furthermore, the connecting pipe is made of polyurethane material.

[0032] Beneficial effects: The urethane material has good flexibility and wear resistance, and can adapt to the deformation of the wearer during exercise or when the tube is bent; at the same time, the polyurethane material also has excellent biocompatibility and will not cause contamination or irritation to the blood, which helps to ensure the long-term stability and safety of the device.

[0033] Furthermore, wearable circulating tumor cell capture devices are used to make equipment for collecting CTCs and clearing CTCs.

[0034] Beneficial effects: The device can not only be used to efficiently collect CTCs, but also to remove CTCs from the blood, providing a powerful tool for early diagnosis, efficacy evaluation and prognosis monitoring of cancer. The wearable and precise control characteristics of the device make it have broad application prospects in cancer management and personalized treatment.

[0035] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0036] Figure 1 Schematic diagram of the structure of a wearable circulating tumor cell capture device in an embodiment of the present invention.

[0037] Figure 2 It is an axonometric view of the magnetic bead control component in an embodiment of the present invention.

[0038] Figure 3It is a front view of the magnetic bead control component in an embodiment of the present invention.

[0039] Figure 4 for Figure 3 Cross-sectional view of the magnetic bead control component along the B-B axis.

[0040] Figure 5 for Figure 3 Cross-sectional view of the magnetic bead control component along the C-C axis.

[0041] Figure 6 1 is a distribution diagram of magnetic flux lines of the electromagnets with the same magnetic poles and the electromagnets with different magnetic poles in the embodiment of the present invention.

[0042] The figure marks in the drawings of the specification include: 1. magnetic bead dispenser; 2. magnetron; 3. magnetic chip; 4. pump assembly; 5. wearable body; 6. three-way; 7. first one-way valve; 8. first filter element; 9. second one-way valve; 10. second filter element; 11. connecting pipe; 12. first electromagnet; 13. second electromagnet. DETAILED DESCRIPTION

[0043] The following is further described in detail through specific implementation methods:

[0044] Example 1

[0045] As attached Figure 1-Figure 6 As shown: a wearable circulating tumor cell capture device, including a magnetic chip 3, a magnetic bead dispenser 1 and a controller; a connecting tube 11 is provided between the magnetic bead dispenser 1 and the magnetic chip 3, the output end of the magnetic bead dispenser 1 and the input end of the magnetic chip 3 are unidirectionally connected through the connecting tube 11, the material of the connecting tube 11 is made of polyurethane material, and the controller is used to control the magnetic bead dispenser 1 to dispense magnetic beads.

[0046] The output end of the magnetic chip 3 is connected to the pump assembly 4, and the controller is used to control the operation of the pump assembly 4; the output end of the pump assembly 4 is connected to the first venous catheter, the end of the first venous catheter away from the pump assembly 4 is connected to the wearable body 5, the end of the wearable body 5 away from the first venous catheter is connected to the second venous catheter, and the end of the second venous catheter away from the wearable body 5 is connected to the position where the connecting tube 11 and the output end of the magnetic bead dispenser 1 are connected.

[0047] A magnetron 2 with a rectangular structure is connected to the outer surface of the connecting tube 11 . A magnetic bead control component for controlling the trajectory of magnetic beads passing through the connecting tube 11 is arranged inside the magnetron 2 .

[0048] The magnetic bead control assembly includes a magnetron 2, which is sleeved on a connecting tube 11. A first groove is horizontally symmetrically opened in the magnetron 2 along its length direction. A plurality of first electromagnets 12 are fixedly connected in the first grooves by bolts. The controller is used to control the magnetic pole reversal of the first electromagnets 12. Specifically, the polarity of the first electromagnet 12 is determined by the current. The controller changes the direction of the current to change the magnetic pole of the first electromagnet 12. When the magnetic beads flow through the magnetron 2, the controller controls the current flowing into the first electromagnet 12 to adjust the distribution of the magnetic beads in the connecting tube 11, including:

[0049] The controller controls the current directions of the horizontally symmetrical and adjacent first electromagnets 12 to be the same, so that the opposite ends of the two first electromagnets 12 are the same poles, so that the magnetic flux lines between the first electromagnets 12 are diffusely distributed, and the magnetic beads are diffusely distributed in the connecting tube 11 along the magnetic flux lines between the first electromagnets 12; the controller controls the current directions of the horizontally symmetrical and adjacent first electromagnets 12 to be opposite, so that the opposite ends of the two first electromagnets 12 are opposite poles, so that the magnetic flux lines between the first electromagnets 12 are converged, and the magnetic beads are linearly converged in the connecting tube along the magnetic flux lines between the first electromagnets.

[0050] A second groove is formed in the magnetron 2 along its length direction, and a plurality of second electromagnets 13 are fixedly connected in the second groove by bolts. The controller is used to control the opening and closing of the second electromagnets 13 .

[0051] A first one-way valve 7 is connected in the first venous catheter, and a first filter element 8 is connected between the first one-way valve 7 and the wearable body 5 in the first venous catheter; a second one-way valve 9 is connected in the second venous catheter, and a second filter element 10 is connected between the second one-way valve 9 and the connecting tube 11 in the second venous catheter; the second venous catheter, the output end of the magnetic bead dispenser 1 and the connecting tube 11 are interconnected through a tee 6.

[0052] The specific implementation process is as follows:

[0053] First, the wearable circulating tumor cell capture device is worn on the appropriate part of the patient, connected to the venous system of the wearable subject 5 through the first venous catheter and the second venous catheter, and the controller is started. The controller controls the magnetic beads with specific antibodies of the magnetic bead dispenser 1 to enter the connecting tube 11, and the controller controls the pump assembly 4 to start. In this embodiment, the pump assembly 4 is a peristaltic pump; driven by the pump assembly 4, the blood of the wearable subject 5 can enter the connecting tube 11 from the second venous tube through the second one-way valve 9 and the second filter element 10, and then enter the magnetic chip 3 through the connecting tube 11 through the magnetron 2, and then enter the input end of the peristaltic pump through the magnetic chip 3, and then enter the first venous catheter from the output end of the peristaltic pump through the first one-way valve 7 and the first filter element 8 and then return to the body of the wearable subject 5.

[0054] When the magnetic bead dispenser 1 releases magnetic beads, these magnetic beads flow in the connecting tube 11 along with the blood circulation of the wearer 5; the connecting tube 11 is made of polyurethane material, which has good flexibility and wear resistance, and can adapt to the deformation of the connecting tube 11 when the patient moves or changes posture, while maintaining the smooth flow of blood and magnetic beads.

[0055] When the magnetic beads flow through the magnetron 2, the controller controls the magnetic pole reversal of the first electromagnet 12, using the principle that the magnetic flux lines of electromagnets with the same magnetic poles and electromagnets with different magnetic poles are distributed differently. When the magnetic poles of two first electromagnets 12 that are horizontally symmetrical and adjacent are the same, the distribution of the magnetic flux lines is as follows: Figure 6 As shown, at this time, the magnetic flux lines between the first electromagnets 12 are diffusely distributed. Since the diameter of the magnetic beads is 4 μm, the magnetic beads can be diffusely distributed in the connecting tube 11 along the magnetic flux lines between the first electromagnets 12, which greatly increases the capture efficiency of the magnetic beads for CTCs and avoids the aggregation of a large number of magnetic beads due to the magnetic effect between the magnetic beads, which affects the capture of CTCs.

[0056] When the magnetic poles of two adjacent first electromagnets 12 are horizontally symmetrical and have opposite names, the magnetic flux lines will be distributed linearly; at this time, the magnetic beads will be attracted by the magnetic force and move linearly along the magnetic flux lines. Since the diameter of the magnetic beads is 4μm, they can be arranged linearly and aggregated along these converging magnetic flux lines. At the same time, this arrangement helps the magnetic beads to more effectively contact the circulating tumor cells (CTCs) flowing through the connecting tube 11, thereby improving the efficiency of the magnetic beads in capturing CTCs.

[0057] The present invention can achieve precise control over the distribution of magnetic beads in the connecting tube 11. By adjusting the magnetic poles of the first electromagnet 12, the magnetic beads can be guided to be distributed along specific magnetic flux lines, ensuring that the magnetic beads are evenly distributed during blood circulation and avoiding aggregation of the magnetic beads. At the same time, when the connecting tube 11 is deformed due to movement of the patient, the magnetic beads can still be evenly distributed. This solves the problem that when the wearable body 5 moves or the tube is displaced, bent or deformed, the magnetic beads may aggregate or move irregularly at a certain position in the tube under the action of gravity and centrifugal force, thereby greatly affecting the efficiency of the magnetic beads in capturing CTCs.

[0058] At the same time, the controller can control the opening and closing of the second electromagnet 13 in sequence, so that the magnetic beads can move in sequence along the second electromagnet 13, thereby realizing the controllability of the movement trajectory of the magnetic beads; this design not only improves the capture efficiency of the magnetic beads, but also provides more flexibility for subsequent CTCs detection and analysis.

[0059] Specifically, the magnetic chip 3 may be integrated with a sensor or detection system for real-time monitoring of the number of magnetic beads adsorbed on the chip surface and whether they have successfully bound to CTCs. When it is detected that the number of CTCs captured by the magnetic beads is insufficient or unevenly distributed, the controller can automatically adjust the residence time and arrangement of the magnetic beads based on this information.

[0060] The controller can change the residence time of the magnetic beads in the connecting tube 11 and the magnetic chip 3 by adjusting the power-on time of the two adjacent symmetrical first electromagnets 12, thereby achieving controllable adjustment of the residence time, increasing the contact opportunities between the magnetic beads and CTCs, and improving the capture efficiency.

[0061] The controller can also change the different arrangements of the magnetic beads in the connecting tube 7 by adjusting the power-on time and reversing of the magnetic poles of each first electromagnet 12 according to the number of CTCs captured by the magnetic beads detected by the magnetic chip, so as to optimize the arrangement and residence time of the magnetic beads in the connecting tube 11. This dynamic adjustment can ensure the maximum contact efficiency between the magnetic beads and CTCs during the blood circulation process.

[0062] The magnetron 2 serves as an outer connection structure of the connecting tube 11. The change of its position and angle can affect the control effect of the electromagnet on the magnetic beads. That is, by changing the position of the magnetron 2 and rotating the magnetron 2 outside the connecting tube 7, the angle and relative position of the first electromagnet 12 and the second electromagnet 13 can be changed accordingly, thereby realizing the movement and arrangement of the magnetic beads at different angles in the connecting tube 11.

[0063] By adjusting the position of the magnetron 2, the direction and strength of the magnetic field generated by the electromagnet can be changed, thereby guiding the magnetic beads to move along different paths in the connecting tube 11. This control can make the magnetic beads more flexible to adapt to the complex structure of the blood circulation system and improve the capture efficiency.

[0064] Changing the angle of the electromagnet can also achieve different angles of arrangement of the magnetic beads in the connecting tube 11. For example, by adjusting the tilt angle of the electromagnet, the magnetic beads can be arranged in an inclined linear distribution or a spiral distribution in the connecting tube 11, thereby increasing the contact area and opportunity between the magnetic beads and CTCs.

[0065] At the same time, the sequential opening and closing of the second electromagnet 13 can realize the controllability of the movement trajectory of the magnetic beads; in order to further optimize this process, a mechanism for sequentially energizing the electromagnets is designed to realize the auxiliary input of the magnetic beads to the chip.

[0066] At the same time, the controller can also transport a part of the magnetic beads to the magnetic chip 3 through the second electromagnet 13 by controlling the opening and closing of the second electromagnet 13 in sequence, and then feedback the control of the first electromagnet 12 on the distribution and residence time of the magnetic beads by detecting the capture effect of this part of the magnetic beads on CTCs. When it is detected that a part of the magnetic beads capture a small number of CTCs, the controller can control the first electromagnet 12 to increase the pole reversing frequency, increase the residence time of the magnetic beads, change the position of the magnetron, rotate the magnetron, and other operations to further increase the capture efficiency of the magnetic beads for CTCs.

[0067] After the magnetic beads bind to CTCs, the magnetic beads flow through the magnetic chip 3, and the magnetic chip 3 uses the magnetic field to adsorb the magnetic beads bound to CTCs on the chip surface to capture CTCs; the captured CTCs can be used for subsequent cancer diagnosis, efficacy evaluation and prognosis monitoring.

[0068] The pump assembly 4 continuously drives the mixture of blood and magnetic beads to circulate in the device. The first one-way valve 7 in the first venous catheter ensures that blood can only flow in one direction, from the wearable body 5 to the magnetic chip 3, preventing the reduction of capture efficiency and possible infection risks caused by blood backflow; at the same time, the first filter 8 effectively removes impurities and larger particles in the blood to prevent them from interfering with the capture of CTCs by magnetic beads.

[0069] After being processed by the magnetic chip 3, the blood returns to the wearable body 5 through the second venous catheter. The second one-way valve 9 also ensures that the blood can only flow in one direction, from the magnetic chip 3 back to the wearable body 5, which helps to maintain the blood flow balance of the system; the second filter 10 further purifies the blood, removes possible residual magnetic beads or other impurities, and protects the patient's vascular system.

[0070] Finally, when the number of captured CTCs reaches a preset value, the operation of the device is stopped, and subsequent CTCs detection and analysis are performed.

[0071] Example 2

[0072] The difference from the above embodiment is that based on the application of the above wearable circulating tumor cell capture device, a device for collecting CTCs and clearing CTCs is produced.

[0073] Obviously, the above embodiments are merely examples for the purpose of clear explanation, and are not intended to limit the implementation methods. For those skilled in the art, other different forms of changes or modifications can be made based on the above description. It is not necessary and impossible to list all the implementation methods here. The obvious changes or modifications derived therefrom are still within the scope of protection of the invention.

Claims

1. A wearable circulating tumor cell capture device, comprising a magnetic chip (3) and a magnetic bead dispenser (1), characterized in that: It also includes a controller; a connecting tube (11) is provided between the magnetic bead dispenser (1) and the magnetic chip (3); the output end of the magnetic bead dispenser (1) and the input end of the magnetic chip (3) are unidirectionally connected via the connecting tube (11); the controller is used to control the magnetic bead dispenser (1) to dispense magnetic beads; the output end of the magnetic chip (3) is connected to a pump assembly (4); the controller is used to control the operation of the pump assembly (4); the output end of the pump assembly (4) is connected to a first venous catheter; the end of the first venous catheter away from the pump assembly (4) is connected to a wearable body (5); the end of the wearable body (5) away from the first venous catheter is connected to a second venous catheter; the end of the second venous catheter away from the wearable body (5) is connected to the connecting tube (11) and the position where the output end of the magnetic bead dispenser (1) is connected; A magnetron (2) in a rectangular structure is connected to the outer shell of the connecting tube (11), and a magnetic bead control component for controlling the trajectory of magnetic beads passing through the connecting tube (11) is provided in the magnetron (2).

2. The wearable circulating tumor cell capture device according to claim 1, characterized in that: The magnetic bead control component comprises a magnetron (2), the magnetron (2) is sleeved on a connecting tube (11), a first groove is horizontally symmetrically opened in the magnetron (2) along its length direction, a plurality of first electromagnets (12) are fixedly connected in the first grooves, and a controller is used to control the magnetic pole reversal of the first electromagnets (12).

3. The wearable circulating tumor cell capture device according to claim 2, characterized in that: A second groove is formed in the magnetron (2) along its length direction, and a plurality of second electromagnets (13) are fixedly connected in the second groove. The controller is used to control the opening and closing of the second electromagnets (13).

4. The wearable circulating tumor cell capture device according to claim 3, characterized in that: The first venous catheter is connected to a first one-way valve (7), and the first filter element (8) is connected between the first one-way valve (7) in the first venous catheter and the wearable body (5).

5. The wearable circulating tumor cell capture device according to claim 1, characterized in that: When the magnetic beads flow through the magnetron (2), the controller controls the current supplied to the first electromagnet (12) to adjust the distribution of the magnetic beads in the connecting tube (11), including: The controller controls the current directions of the horizontally symmetrical and adjacent first electromagnets (12) to be the same, so that the opposite ends of the two first electromagnets (12) are the same magnetic poles, so that the magnetic flux lines between the first electromagnets (12) are diffusely distributed, and the magnetic beads are diffusely distributed along the magnetic flux lines between the first electromagnets (12) in the connecting tube; the controller controls the current directions of the horizontally symmetrical and adjacent first electromagnets (12) to be opposite, so that the opposite ends of the two first electromagnets (12) are the opposite magnetic poles, so that the magnetic flux lines between the first electromagnets (12) are convergently distributed, and the magnetic beads are linearly convergently distributed along the magnetic flux lines between the first electromagnets (12) in the connecting tube.

6. The wearable circulating tumor cell capture device according to claim 5, characterized in that: The second venous catheter is connected to a second one-way valve (9).

7. The wearable circulating tumor cell capture device according to claim 6, characterized in that: A second filter element (10) is connected between the second one-way valve (9) and the connecting pipe (11) in the second venous catheter.

8. The wearable circulating tumor cell capture device according to claim 7, characterized in that: The second venous catheter, the output end of the magnetic bead dispenser (1) and the connecting tube (11) are interconnected via a tee (6).

9. The wearable circulating tumor cell capture device according to claim 8, characterized in that: The connecting pipe (11) is made of polyurethane material.

10. Use of the wearable circulating tumor cell capture device according to any one of claims 1 to 9, characterized in that: Wearable circulating tumor cell capture devices are used to make devices for collecting CTCs and clearing CTCs.

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