Cattle lung active substance elution and separation equipment

Abstract

The invention belongs to the technical field of bioactive substance extraction equipment, particularly relates to bovine lung active substance elution and separation equipment, and provides the following scheme aiming at the problems that existing equipment cannot realize automatic operation of the whole elution and extraction process and lacks of early-stage filtration treatment on eluent. Comprising a supporting bottom plate, an elution and extraction assembly, liquid storage barrels and a centrifugal separation assembly, all the assemblies of the elution and separation equipment can be integrated and combined together through the supporting bottom plate, and eluent in the cattle lung is pumped into one of the liquid storage barrels through the elution and extraction assembly; one liquid storage barrel filled with the eluant can rotate under the action of the centrifugal separation assembly, the other liquid storage barrel filled with the eluant can rotate under the action of the centrifugal separation assembly, then the eluant in the barrels can be centrifugally separated, active substances in the cattle lungs can be extracted, the two liquid storage barrels can enable the two processes of eluant collection and centrifugation to be carried out at the same time, and the extraction efficiency is improved. The extraction efficiency of the elution and separation equipment on active substances in the cattle lung can be greatly improved.

Description

Technical Field

[0001] The invention relates to an elution and separation device, in particular to a bovine lung active substance elution and separation device, belonging to the technical field of biological active substance extraction equipment. Background Art

[0002] Bovine lung surfactant refers to a complex lipoprotein secreted by alveolar type II epithelial cells, whose main components are dipalmitoylphosphatidylcholine and surfactant binding protein. The extracted bovine lung surfactant can be used to prepare bovine lung surfactant for injection. Bovine lung surfactant is a therapeutic drug for respiratory diseases and is used for premature infants and adult patients with respiratory distress syndrome. Premature infants refer to newborns with a gestational age of 28 to less than 37 weeks (weight <2500g); adult respiratory distress syndrome is a group of clinical syndromes characterized by alveolar collapse and reduced lung compliance caused by various reasons. The use of the above-mentioned drugs can improve the ventilation function of premature infants and adults and increase the survival rate. Because the injection of bovine lung surfactant can stabilize the alveolar membrane, increase the alveolar surface tension, and thus improve the ventilation function, it has a good effect on respiratory distress in premature infants and adults.

[0003] The extraction of active substances in cattle lungs requires elution operation. The traditional operation method is to cut the main trachea of ​​the cattle horizontally, insert a plastic tube into the trachea 6-8cm and tie it tightly with a thin wire, and connect the other end to a three-way pipe. One end of the three-way pipe is connected to a barrel of physiological sodium chloride solution placed on a high platform, and the other end is connected to a receiving bottle under the cattle. Close the pipeline valve between the receiving bottle and the cattle, open the pipeline valve between the cattle and the barrel of physiological sodium chloride solution, and pour the physiological sodium chloride solution into the cattle's lungs. After the cattle's lungs are completely filled, close the pipeline valve between the cattle and the barrel of physiological sodium chloride solution, gently shake the cattle for 3 minutes, open the pipeline valve between the receiving bottle and the cattle, and put the liquid in the lungs into the receiving bottle. Repeat the above method to lavage until the total amount of lung lavage fluid obtained for each calf is 10-10.5L. Pour the lavage fluid into a sterile centrifuge bottle, centrifuge at 800-1000 rpm for 10 minutes to remove impurities such as epithelium and cells, pour the supernatant into a lavage fluid bucket to obtain bovine lung lavage fluid, and freeze it at low temperature (no higher than -20°C) for storage until use.

[0004] In the prior art, there is a separation device for extracting active substances from a large-capacity lung lavage recovery fluid, as disclosed in announcement number CN201023028Y, which is provided with a recovery liquid tank connected to a microfiltration filter by connecting a microfiltration membrane pump; the microfiltration filter is connected to a nanofiltration tank through a series-connected ultrafiltration membrane assembly; and the nanofiltration tank is connected to the nanofiltration membrane assembly by connecting a nanofiltration membrane pump. In addition, the equipment can be used for the separation and extraction of various effective components of lung lavage recovery fluids of humans, cows, pigs, etc. Although the existing equipment can also separate and extract active substances from cow lungs, the existing equipment only has a separation function, and does not involve how to extract the eluate from cow lungs. Therefore, in actual use, personnel are required to extract the eluate, and then filter and extract it with a microfiltration membrane. The entire elution and extraction process cannot be automated, and the efficiency is low. Secondly, although the existing equipment uses an ultrafiltration membrane to extract active substances, the eluate often contains more impurities. Therefore, if it is directly filtered through an ultrafiltration membrane, it will seriously affect the filtration effect of the microfiltration membrane, and will also cause the entire extraction and separation process to be relatively slow. Based on this, the present application proposes a bovine lung active substance elution and separation device. Summary of the invention

[0005] The invention provides a bovine lung active substance elution and separation device to solve the problems that the existing equipment cannot realize the automated operation of the entire elution and extraction process and lacks the early filtering treatment of the eluate.

[0006] The present invention achieves the above-mentioned purpose through the following technical scheme: a bovine lung active substance elution and separation device, comprising a supporting bottom plate, an elution and extraction component, a liquid storage barrel and a centrifugal separation component, wherein the elution and extraction component, the liquid storage barrel and the centrifugal separation component are all arranged on the supporting bottom plate, two liquid storage barrels are symmetrically arranged, the output end of the elution and extraction component is located directly above one of the liquid storage barrels, and the centrifugal separation component is connected to one side of the other liquid storage barrel; The elution extraction component includes an irrigating liquid pumping tank and an eluent pumping tank, the irrigating liquid pumping tank and the eluent pumping tank are arranged side by side, the bottom end of the irrigating liquid pumping tank is connected with an irrigating liquid extraction pipe and an irrigating liquid injection pipe, the bottom end of the eluent pumping tank is connected with an eluent extraction pipe and an eluent delivery pipe, a three-way pipe is connected between the irrigating liquid injection pipe and the eluent extraction pipe, the other interface of the three-way pipe is connected with the eluent injection pipe, and each pipeline connected between the irrigating liquid pumping tank and the bottom end of the eluent pumping tank is provided with a one-way valve unit; A filter assembly is installed on the pipe body of the eluent delivery pipe. The filter assembly includes a fixed shell and a movable shell. The movable shell is movably connected to the bottom end of the fixed shell. Filter units are arranged in the fixed shell and the movable shell.

[0007] As a further solution of the present invention: a supporting bottom rod is fixedly connected to the four corners of the bottom of the supporting bottom plate, a limiting fixing plate is arranged parallel to the top of the supporting bottom plate, the limiting support rods are fixedly connected to the four corners of the limiting fixing plate, the bottom end of the limiting support rod is fixedly connected to the upper plate surface of the supporting bottom plate, and the tank bodies of the irrigation fluid pumping tank and the elution fluid pumping tank are both fixedly connected to the limiting fixing plate.

[0008] As a further solution of the present invention: a movable push rod and a piston are provided in both the irrigation liquid pumping tank and the eluent pumping tank, and the movable push rod is coaxially fixedly connected to the top of the piston.

[0009] As a further solution of the present invention: a synchronous connecting plate is fixedly connected between the upper ends of the two movable push rods, a hydraulic push rod is arranged between the irrigation fluid pumping tank and the eluent pumping tank, the bottom end of the hydraulic push rod is fixedly connected to the limit fixing plate, and the movable upper end of the hydraulic push rod is fixedly connected to the lower plate surface of the synchronous connecting plate.

[0010] As a further solution of the present invention: the one-way valve unit includes an irrigation liquid extraction tube one-way valve, an irrigation liquid injection tube one-way valve, an eluent extraction tube one-way valve and an eluent delivery tube one-way valve, the irrigation liquid extraction tube one-way valve is fixedly connected in the irrigation liquid extraction tube, the irrigation liquid injection tube one-way valve is fixedly connected in the irrigation liquid injection tube, the eluent extraction tube one-way valve is fixedly connected in the eluent extraction tube, and the eluent delivery tube one-way valve is fixedly connected in the eluent delivery tube; the upper plate surface of the supporting base plate is fixedly connected with an irrigation liquid storage tank, the irrigation liquid extraction tube is inserted in the irrigation liquid storage tank, and the irrigation liquid storage tank stores irrigation liquid.

[0011] As a further solution of the present invention, a top cover is movably mounted on the upper end of the fixed housing of the filter assembly, the fixed housing is connected to the eluent delivery pipe, and the bottom end of the movable housing of the filter assembly is connected to a filtrate discharge pipe; the outlet of the filtrate discharge pipe is located directly above one of the liquid storage barrels; The bottom outer wall of the fixed shell is provided with an external thread docking surface, the upper inner wall of the movable shell is provided with an internal thread docking surface, and the external thread docking surface is threadedly docked with the internal thread docking surface.

[0012] As a further solution of the present invention: the filter unit includes a conical filter, a funnel-shaped filter and a cylindrical filter, the conical filter is fixedly connected to the center of the bottom surface of the top cover, the funnel-shaped filter is fixedly connected to the upper opening of the movable shell, the cylindrical filter is integrally connected to the bottom of the funnel-shaped filter, and the conical filter is placed directly above the cylindrical filter.

[0013] As a further solution of the present invention: a rotating base plate is movably arranged on the supporting base plate, and a rotating motor is fixedly connected to the lower plate surface of the supporting base plate. The rotating shaft of the rotating motor passes through the supporting base plate, and the upper end of the rotating shaft of the rotating motor is fixedly connected to the center of the lower plate surface of the rotating base plate. A rotating bearing is sleeved on the rotating shaft body of the rotating motor passing through the supporting base plate, and barrel bottom limit seats are symmetrically connected to both ends of the upper plate surface of the rotating base plate, and the bottom end of the liquid storage barrel is clamped in the barrel bottom limit seat.

[0014] As a further solution of the present invention: the bottom surface of the barrel bottom limit seat is connected to a compression air bag, the inner wall of the barrel bottom limit seat is connected to an annular expansion air bag, an air guide channel is opened in the barrel bottom limit seat, and the compression air bag and the annular expansion air bag are connected through the air guide channel; A centrifugal connecting rod is fixedly connected to the bottom center of the barrel bottom limit seat, the centrifugal connecting rod passes through the rotating bottom plate, and a centrifugal bearing is sleeved on the rod body of the centrifugal connecting rod passing through the rotating bottom plate. A fixed friction plate is coaxially fixedly connected to one end of the centrifugal connecting rod located below the rotating bottom plate.

[0015] As a further solution of the present invention: the centrifugal separation component comprises a hydraulic outer rod and a hydraulic inner rod, the hydraulic inner rod is movably inserted at both ends of the hydraulic outer rod, the rod body of the hydraulic outer rod is connected to the support bottom plate, the hydraulic inner rod connected to the upper end of the hydraulic outer rod is fixedly connected to a fixed clamping plate, the lower plate surface of the fixed clamping plate is movably connected to a barrel top positioning piston, the barrel top positioning piston is located directly above the liquid storage barrel, the hydraulic inner rod connected to the lower end of the hydraulic outer rod is fixedly connected to a centrifugal motor, the rotating shaft of the centrifugal motor passes through the support bottom plate, the upper end of the rotating shaft of the centrifugal motor is coaxially fixedly connected to a movable friction plate, and the movable friction plate is located directly below the fixed friction plate; A docking shaft is coaxially connected between the fixed clamping plate and the barrel top positioning piston, an upper annular groove is provided on the lower plate surface of the fixed clamping plate, a lower annular groove is provided on the upper end surface of the barrel top positioning piston, and a plurality of balls are clamped between the upper annular groove and the lower annular groove; The two ends of the rotating bottom plate are fixedly connected with limit top blocks, and the rod body of the hydraulic outer rod of the centrifugal separation component close to the rotating bottom plate is fixedly connected with a limit switch.

[0016] The beneficial effects of the present invention are: 1. The present invention is provided with a supporting bottom plate, an elution and extraction component, a liquid storage barrel and a centrifugal separation component, and the components of the elution and separation device can be integrated and combined together through the supporting bottom plate, and the eluate in the bovine lung is pumped into one of the liquid storage barrels through the elution and extraction component, and the other liquid storage barrel already filled with eluate can be rotated under the action of the centrifugal separation component, and then the eluate in the barrel can be centrifuged to achieve the extraction of active substances in the bovine lung. The two liquid storage barrels can make the collection and centrifugation of the eluate be carried out simultaneously, which can greatly improve the efficiency of the elution and separation device for extracting active substances from the bovine lung; 2. The elution extraction assembly provided in the present invention includes an irrigating liquid pumping tank and an irrigating liquid pumping tank. The bottom end of the irrigating liquid pumping tank is connected with an irrigating liquid extraction pipe and an irrigating liquid injection pipe. The bottom end of the irrigating liquid pumping tank is connected with an irrigating liquid extraction pipe and an irrigating liquid delivery pipe. The irrigating liquid pumping tank and each pipe connected with the bottom end of the irrigating liquid pumping tank are provided with a one-way valve unit. The irrigating liquid can be pumped into the irrigating liquid pumping tank through the irrigating liquid extraction pipe. At the same time, the irrigating liquid extraction pipe can pump the irrigating liquid in the bovine lung into the elution tank. The lavage liquid pumping tank is then injected into the bovine lung through the lavage liquid injection tube. At the same time, the eluent delivery tube transports the eluent in the lavage liquid pumping tank to the eluent injection tube, and then the eluent injection tube injects the eluent into the liquid storage barrel. The one-way valve unit is set to ensure that each pipeline can only be pumped or transported in one direction, so that the pumping of the lavage liquid and the pumping of the eluent are carried out synchronously, which can also improve the efficiency of the elution separation device in extracting active substances from bovine lungs; 3. A filter assembly is installed on the tube body of the eluent injection tube provided in the present invention, and the filter assembly includes a fixed shell and a movable shell. Filter units are arranged in the fixed shell and the movable shell. The eluent can be filtered through the filter unit of the filter assembly to remove some debris in the bovine lung, thereby ensuring that the eluent injected into the liquid storage barrel does not contain many impurities, so as to facilitate the centrifugal separation and extraction of bovine lung active substances. At the same time, the detachable and movable connection between the fixed shell and the movable shell also facilitates the removal of the filtered debris to avoid long-term use affecting the filtering effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0017] Figure 1 It is a schematic diagram of the overall structure of the present invention; Figure 2 This is a schematic diagram of the structure of the elution and extraction component of the present invention; Figure 3 This is a schematic diagram of the cross-sectional structure of the irrigation liquid pumping tank of the present invention; Figure 4 This is a schematic diagram of the structure of the eluent pumping tank of the present invention; Figure 5 It is a schematic diagram of the liquid flow structure of the movable push rod of the elution and extraction component of the present invention in the lifted state; Figure 6 It is a schematic diagram of the liquid flow structure of the elution and extraction component of the present invention when the movable push rod is pressed down; Figure 7 This is a schematic diagram of the structure of the filter assembly of the present invention; Figure 8 This is a schematic diagram of the cross-sectional structure of the filter assembly of the present invention; Fig. 9 It is a schematic diagram of the combined structure of the rotating bottom plate and the centrifugal separation assembly of the present invention; Fig.10 This is a schematic diagram of the structure of the rotating bottom plate of the present invention; Fig.11 This is a schematic diagram of the cross-sectional structure of the rotating bottom plate of the present invention; Fig.12 For the present invention Fig.11 Schematic diagram of the structure at A in the middle; Fig.13 It is a schematic diagram of the structure of the centrifugal separation component of the present invention; Fig.14 It is a schematic diagram of the cross-sectional structure of the fixed clamping plate and the barrel top positioning piston of the present invention; Fig.15 It is a schematic diagram of the connection structure between the hydraulic outer rod and the hydraulic inner rod of the present invention.

[0018] In the figure: 1. Support bottom plate, 11. Support bottom rod, 12. Limit fixing plate, 13. Limit support rod, 2. Elution extraction component, 21. Irrigation liquid pumping tank, 22. Eluent pumping tank, 23. Movable push rod, 24. Piston, 25. Irrigation liquid extraction tube, 26. Irrigation liquid injection tube, 27. Eluent extraction tube, 28. Eluent delivery tube, 29. Three-way tube, 210. Eluent injection tube, 211. Irrigation liquid extraction tube one-way valve, 212. Irrigation liquid injection tube one-way valve, 213. Eluent extraction tube one-way valve, 214. Eluent delivery tube one-way valve, 215. Synchronous connecting plate, 216. Hydraulic push rod, 3. Irrigation liquid storage tank, 4. Filter component, 41. Filtered liquid discharge tube, 42. Fixed shell, 43. Movable shell, 44. Top cover, 45. Conical filter, 46. External thread docking surface, 47. Internal thread docking surface, 48. Funnel-shaped filter, 49. Cylindrical filter, 5. Liquid storage barrel, 6. Rotating bottom plate, 61. Rotating motor, 62. Rotating bearing, 63. Barrel bottom limit seat, 64. Centrifugal connecting rod, 65. Fixed friction plate, 66. Centrifugal bearing, 67. Compression airbag, 68. Air guide channel, 69. Annular expansion airbag, 610. Limit top block, 7. Centrifugal separation component, 71. Hydraulic outer rod, 72. Hydraulic inner rod, 73. Fixed clamping plate, 74. Centrifugal motor, 75. Barrel top positioning piston, 76. Movable friction plate, 77. Upper annular groove, 78. Lower annular groove, 79. Ball, 710. Docking shaft, 711. Limit switch. DETAILED DESCRIPTION

[0019] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0020] Embodiment 1 like Figure 1 , Figure 2 , Figure 3 , Figure 4 and Figure 7 As shown, a bovine lung active substance elution and separation device comprises a supporting base plate 1, an elution and extraction component 2, a liquid storage barrel 5 and a centrifugal separation component 7. The elution and extraction component 2, the liquid storage barrel 5 and the centrifugal separation component 7 are all arranged on the supporting base plate 1. Two liquid storage barrels 5 are symmetrically arranged. The output end of the elution and extraction component 2 is located directly above one of the liquid storage barrels 5. The centrifugal separation component 7 is connected to one side of the other liquid storage barrel 5, so that the various components of the elution and separation device can be integrated and combined together through the supporting base plate 1, and the eluate in the bovine lung is pumped into one of the liquid storage barrels 5 through the elution and extraction component 2, and the other liquid storage barrel already filled with the eluate can be rotated under the action of the centrifugal separation component 7, and then the eluate in the barrel can be centrifuged to achieve the extraction of active substances in the bovine lung. The two liquid storage barrels 5 can make the collection and centrifugation of the eluate be carried out simultaneously, which can greatly improve the efficiency of the elution and separation device for extracting bovine lung active substances; The elution extraction component 2 includes an irrigating liquid pumping tank 21 and an eluting liquid pumping tank 22. The irrigating liquid pumping tank 21 and the eluting liquid pumping tank 22 are arranged side by side. The bottom end of the irrigating liquid pumping tank 21 is connected with an irrigating liquid extraction tube 25 and an irrigating liquid injection tube 26. The bottom end of the eluting liquid pumping tank 22 is connected with an eluent extraction tube 27 and an eluent delivery tube 28. A three-way tube 29 is connected between the irrigating liquid injection tube 26 and the eluent extraction tube 27. The other interface of the three-way tube 29 is connected with an eluent injection tube 210. Each pipeline connected between the bottom ends of the irrigating liquid pumping tank 21 and the eluent pumping tank 22 is provided with a one-way valve unit, which can be used to The valve unit enables the lavage liquid extraction tube 25 to draw the lavage liquid into the lavage liquid pumping tank 21, and then the lavage liquid in the lavage liquid pumping tank 21 is injected into the bovine lung through the lavage liquid injection tube 26, and the eluent extraction tube 27 can draw the eluent in the bovine lung into the eluent pumping tank 22 through the eluent injection tube 210 through the one-way valve unit, and then the eluent delivery tube 28 delivers the eluent in the eluent pumping tank 22 to the liquid storage barrel 5. The one-way valve unit can ensure that each pipeline can only be pumped or delivered in one direction, so that the pumping of the lavage liquid and the eluent can be smoothly carried out; A filter assembly 4 is installed on the body of the eluent delivery pipe 28. The filter assembly 4 includes a fixed shell 42 and a movable shell 43. The movable shell 43 is movably connected to the bottom end of the fixed shell 42. Filter units are arranged in the fixed shell 42 and the movable shell 43. The eluent can be filtered by the filter unit of the filter assembly 4 to remove some impurities in the bovine lung, thereby ensuring that the eluent injected into the liquid storage barrel 5 does not contain many impurities, so as to facilitate the centrifugal separation and extraction of bovine lung active substances. At the same time, the detachable and movable connection between the fixed shell 42 and the movable shell 43 also facilitates the removal of the filtered impurities to avoid long-term use affecting the filtering effect.

[0021] Embodiment 2 Improvements based on Example 1: like Figures 1 to 6 As shown, a supporting bottom rod 11 is fixedly connected at the four corners of the bottom of the supporting bottom plate 1, and a limiting fixing plate 12 is arranged in parallel above the supporting bottom plate 1. A limiting support rod 13 is fixedly connected at the four corners of the limiting fixing plate 12, and the bottom end of the limiting support rod 13 is fixedly connected to the upper plate surface of the supporting bottom plate 1. The tank bodies of the irrigation fluid pumping tank 21 and the eluent pumping tank 22 are both fixedly connected to the limiting fixing plate 12. The elution and separation equipment can be stably supported by the supporting bottom rod 11, and the irrigation fluid pumping tank 21 and the eluent pumping tank 22 can be vertically fixedly connected above the supporting bottom plate 1 through the limiting fixing plate 12, so that the elution and separation equipment can be used normally.

[0022] Furthermore, a movable push rod 23 and a piston 24 are provided in the irrigation fluid pumping tank 21 and the eluent pumping tank 22. The movable push rod 23 is coaxially fixedly connected to the top of the piston 24. The movable push rod 23 can move up and down to drive the piston 24 to move up and down in the irrigation fluid pumping tank 21 and the eluent pumping tank 22 respectively, so as to pump the liquid into the corresponding tank body and discharge the liquid from the corresponding tank body.

[0023] Furthermore, a synchronous connecting plate 215 is fixedly connected between the upper ends of the two movable push rods 23, and a hydraulic push rod 216 is provided between the irrigation liquid pumping tank 21 and the eluent pumping tank 22. The bottom end of the hydraulic push rod 216 is fixedly connected to the limit fixing plate 12, and the movable upper end of the hydraulic push rod 216 is fixedly connected to the lower plate surface of the synchronous connecting plate 215. The synchronous connecting plate 215 can be driven by the hydraulic push rod 216 to move up and down, thereby driving the two movable push rods 23 to move up and down synchronously. Furthermore, when the lavage fluid extraction tube 25 draws the lavage fluid into the lavage fluid pumping tank 21, the eluent extraction tube 27 can simultaneously draw the eluent in the bovine lung into the eluent pumping tank 22, and then the lavage fluid in the lavage fluid pumping tank 21 is injected into the bovine lung via the lavage fluid injection tube 26, while the eluent delivery tube 28 delivers the eluent in the eluent pumping tank 22 to the liquid storage barrel 5, thereby achieving synchronous pumping of the lavage fluid and the eluent, and improving the efficiency of the elution and separation equipment in extracting active substances from bovine lungs.

[0024] Furthermore, the one-way valve unit includes an irrigating liquid extraction tube one-way valve 211, an irrigating liquid injection tube one-way valve 212, an eluent extraction tube one-way valve 213 and an eluent delivery tube one-way valve 214. The irrigating liquid extraction tube one-way valve 211 is fixedly connected to the irrigating liquid extraction tube 25, the irrigating liquid injection tube one-way valve 212 is fixedly connected to the irrigating liquid injection tube 26, and the eluent extraction tube one-way valve 213 is fixedly connected to the eluent extraction tube 27. The one-way valve 214 of the eluent delivery pipe is fixedly connected to the eluent delivery pipe 28 to ensure that when the movable push rod 23 moves upward, the one-way valve 211 of the eluent extraction pipe and the one-way valve 212 of the eluent injection pipe are used to make the eluent extraction pipe 25 extract the eluent, while the eluent injection pipe 26 is in a closed state. At the same time, the one-way valve 213 of the eluent extraction pipe and the one-way valve 214 of the eluent delivery pipe are used to make the eluent The liquid extraction tube 27 extracts eluent from the bovine lung, and the eluent delivery tube 28 is in a closed state, at this time, it can ensure that a certain amount of lavage liquid is stored in the lavage liquid delivery tank 21, and an equal amount of eluent is stored in the eluent delivery tank 22. When the movable push rod 23 moves downward, the lavage liquid extraction tube check valve 211 and the lavage liquid injection tube check valve 212 make the lavage liquid extraction tube 25 in a closed state, and the lavage liquid injection tube 26 can inject the lavage liquid in the tank into the bovine lung. At the same time, the eluent extraction tube check valve 213 and the eluent delivery tube check valve 214 make the eluent extraction tube 27 in a closed state, and the eluent delivery tube 28 can transport the eluent in the tank to the filter assembly 4 for filtering treatment. At this time, it can ensure that a certain amount of lavage liquid is injected into the bovine lung in the lavage liquid delivery tank 21, and an equal amount of eluent is injected into the liquid storage barrel 5; Furthermore, the upper plate surface of the supporting base plate 1 is fixedly connected to an irrigation fluid storage tank 3, and an irrigation fluid extraction tube 25 is inserted in the irrigation fluid storage tank 3. The irrigation fluid storage tank 3 stores irrigation fluid, which can facilitate the irrigation fluid extraction tube 25 to extract the irrigation fluid into the irrigation fluid pumping tank 21. It should be noted that the irrigation fluid stored in the irrigation fluid storage tank 3 is a 20% sodium hydroxide solution.

[0025] like Figure 1 , Figure 2 , Figure 7 and Figure 8 As shown, a top cover 44 is movably mounted on the upper end of the fixed shell 42 of the filter assembly 4, the fixed shell 42 is connected to the eluent delivery pipe 28, and a filtrate discharge pipe 41 is connected to the bottom end of the movable shell 43 of the filter assembly 4, through which the filtered eluent can be discharged, and the top cover 44 can be easily opened, so that the filter residue in the shell can be easily cleaned without affecting the normal filtering work of the filter assembly 4; Furthermore, the outlet of the filtrate discharge pipe 41 is located directly above one of the liquid storage barrels 5 to prevent the eluent from splashing and ensure that the eluent can be discharged into the liquid storage barrel 5; Furthermore, an external threaded docking surface 46 is provided on the outer wall of the bottom end of the fixed shell 42, and an internal threaded docking surface 47 is provided on the inner wall of the upper end of the movable shell 43. The external threaded docking surface 46 is threadedly docked with the internal threaded docking surface 47. Through the threaded docking of the threaded docking surfaces, it can be ensured that the fixed shell 42 and the movable shell 43 are firmly combined and connected together, and it is convenient to disassemble the movable shell 43 for cleaning, ensuring that the filter residue is completely cleaned after use.

[0026] Furthermore, the filter unit includes a conical filter 45, a funnel-shaped filter 48 and a cylindrical filter 49. The conical filter 45 is fixedly connected to the center of the bottom surface of the top cover 44, the funnel-shaped filter 48 is fixedly connected to the upper end opening of the movable shell 43, and the cylindrical filter 49 is integrally connected to the bottom of the funnel-shaped filter 48. The conical filter 45 is placed directly above the cylindrical filter 49 so that the eluent injected by the eluent injection pipe 210 can enter the shell of the filter assembly 4. When inside the body, the eluent is filtered by the combined action of the conical filter 45 and the funnel-shaped filter 48, so that the filtered eluent flows out from the bottom of the funnel-shaped filter 48 and is discharged from the filtered liquid discharge pipe 41. When the top cover 44 is lifted, the conical filter 45 is removed at the same time, and the filter residue falls into the cylindrical filter 49. At this time, the funnel-shaped filter 48 can still achieve a good filtering effect, and it is easy to take out the filter residue in the cylindrical filter 49, which is convenient for operation and use.

[0027] like Figure 1 , Figures 9 to 15 As shown, a rotating base plate 6 is movably arranged on the supporting base plate 1, and a rotating motor 61 is fixedly connected to the lower plate surface of the supporting base plate 1. The rotating shaft of the rotating motor 61 passes through the supporting base plate 1, and the upper end of the rotating shaft of the rotating motor 61 is fixedly connected to the center of the lower plate surface of the rotating base plate 6. A rotating bearing 62 is sleeved on the rotating shaft body of the rotating motor 61 passing through the supporting base plate 1, and the two ends of the upper plate surface of the rotating base plate 6 are symmetrically connected with barrel bottom limit seats 63, and the bottom ends of the liquid storage barrels 5 are clamped in the barrel bottom limit seats 63. The two liquid storage barrels 5 can be limited and placed by the barrel bottom limit seats 63, and the rotating base plate 6 can be driven by the rotating motor 61 to rotate, and then the positions of the two liquid storage barrels 5 can be adjusted, so that the liquid storage barrels 5 filled with eluent can be moved to the centrifugal separation assembly 7 for centrifugal separation of the eluent, and at the same time, the liquid storage barrel 5 after centrifugation can be easily removed to take out the supernatant after centrifugation, and the eluent can be reloaded, so as to realize the simultaneous collection and centrifugation of the eluent.

[0028] Furthermore, a compression airbag 67 is connected to the bottom surface of the barrel bottom limit seat 63, and an annular expansion airbag 69 is connected to the inner wall of the barrel bottom limit seat 63. An air guide channel 68 is provided in the barrel bottom limit seat 63, and the compression airbag 67 and the annular expansion airbag 69 are connected through the air guide channel 68, so that when the liquid storage barrel 5 is placed in the barrel bottom limit seat 63, the compression airbag 67 is compressed, and the gas in the compression airbag 67 flows into the annular expansion airbag 69 through the air guide channel 68, so that the annular expansion airbag 69 expands outward, forming an annular fixation on the bottom end of the liquid storage barrel 5, ensuring that the liquid storage barrel 5 can be firmly placed in the barrel bottom limit seat 63; Furthermore, a centrifugal connecting rod 64 is fixedly connected to the bottom center of the barrel bottom limit seat 63, and the centrifugal connecting rod 64 passes through the rotating bottom plate 6. A centrifugal bearing 66 is sleeved on the rod body of the centrifugal connecting rod 64 that passes through the rotating bottom plate 6. One end of the centrifugal connecting rod 64 located below the rotating bottom plate 6 is coaxially fixedly connected to a fixed friction plate 65. The barrel bottom limit seat 63 can be driven to rotate by the centrifugal connecting rod 64, and then the liquid storage barrel 5 can be driven to rotate, that is, the eluent in the barrel can be centrifuged, and the fixed friction plate 65 can be easily connected to the driving end of the centrifugal separation component 7 to provide driving force for the centrifugal connecting rod 64.

[0029] Furthermore, the centrifugal separation assembly 7 includes a hydraulic outer rod 71 and a hydraulic inner rod 72. The hydraulic inner rod 72 is movably inserted at both ends of the hydraulic outer rod 71. The rod body of the hydraulic outer rod 71 is connected to the support base plate 1. The hydraulic inner rod 72 connected to the upper end of the hydraulic outer rod 71 is fixedly connected to a fixed clamping plate 73. The lower plate surface of the fixed clamping plate 73 is movably connected to a barrel top positioning piston 75. The barrel top positioning piston 75 is located directly above the liquid storage barrel 5. The hydraulic inner rod 72 connected to the lower end of the hydraulic outer rod 71 is fixedly connected to a centrifugal motor 74. The rotating shaft of the centrifugal motor 74 passes through the support base plate 1. The upper end of the rotating shaft of the centrifugal motor 74 is coaxially fixedly connected to The movable friction plate 76 is located directly below the fixed friction plate 65. The hydraulic inner rods 72 at both ends of the hydraulic outer rod 71 can be moved so that the barrel top positioning piston 75 can be stuck in the upper end opening of the liquid storage barrel 5, thereby forming a blockage for the upper end opening of the liquid storage barrel 5 to prevent the eluent from splashing out during the centrifugation process. At the same time, it can also form a fixation for the upper end of the liquid storage barrel 5 to ensure that the liquid storage barrel 5 can rotate stably, and the movable friction plate 76 connected to the centrifugal motor 74 can be tightly attached to the fixed friction plate 65, thereby realizing the transmission of driving force, and driving the liquid storage barrel 5 to rotate along with the barrel bottom limit seat 63; Furthermore, a docking shaft 710 is coaxially connected between the fixed clamping plate 73 and the barrel top positioning piston 75, an upper annular groove 77 is provided on the lower plate surface of the fixed clamping plate 73, and a lower annular groove 78 is provided on the upper end surface of the barrel top positioning piston 75, and a plurality of balls 79 are clamped between the upper annular groove 77 and the lower annular groove 78, so that the barrel top positioning piston 75 can rotate relative to the fixed clamping plate 73 through the action of the docking shaft 710, and the barrel top positioning piston 75 can rotate more stably through the action of the upper annular groove 77 and the lower annular groove 78 in cooperation with the balls 79; Furthermore, the two ends of the rotating base plate 6 are fixedly connected to the limit top blocks 610, and the limit switch 711 is fixedly connected to the rod body of the hydraulic outer rod 71 of the centrifugal separation component 7 close to the side of the rotating base plate 6, so that when the rotating base plate 6 rotates, the limit top block 610 contacts the limit switch 711 to ensure that the rotating base plate 6 rotates into place, so that one of the liquid storage barrels 5 is located just below the barrel top positioning piston 75, ensuring that the centrifugal separation component 7 can clamp the liquid storage barrel 5 and drive the liquid storage barrel 5 to rotate.

[0030] Working principle: the irrigating liquid is pumped into the irrigating liquid pumping tank 21 through the irrigating liquid extraction tube 25, and the eluent extraction tube 27 can pump the eluent in the bovine lung into the eluent pumping tank 22, and then the irrigating liquid in the irrigating liquid pumping tank 21 is injected into the bovine lung through the irrigating liquid injection tube 26, and at the same time the eluent delivery tube 28 delivers the eluent in the eluent pumping tank 22 to the eluent injection tube 210, and then the eluent injection tube 210 injects the eluent into the liquid storage barrel 5, and the one-way valve unit provided can ensure that each pipeline can only be pumped or transported in one direction, so that the pumping of the irrigating liquid and the pumping of the eluent are carried out synchronously; when the eluent injected by the eluent injection tube 210 enters the shell of the filter component 4, the eluent is filtered by the combined action of the conical filter screen 45 and the funnel-shaped filter screen 48, so that the filtered eluent is The eluent flows out from the bottom of the funnel-shaped filter screen 48 and is discharged from the filtrate discharge pipe 41. When the top cover 44 is lifted, the conical filter screen 45 is removed at the same time, and the filter residue will fall into the cylindrical filter screen 49. At this time, the funnel-shaped filter screen 48 can still achieve a good filtering effect. By moving the hydraulic inner rod 72 at both ends of the hydraulic outer rod 71, the barrel top positioning piston 75 can be stuck in the upper end opening of the liquid storage barrel 5, thereby forming a blockage for the upper end opening of the liquid storage barrel 5 to avoid the eluent from splashing out during the centrifugation process. At the same time, it can also form a fixation for the upper end of the liquid storage barrel 5 to ensure that the liquid storage barrel 5 can rotate stably, and the movable friction plate 76 connected to the centrifugal motor 74 can be tightly attached to the fixed friction plate 65, thereby realizing the transmission of driving force, driving the liquid storage barrel 5 to rotate along with the barrel bottom limit seat 63.

[0031] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

[0032] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A bovine lung active substance elution and separation device, comprising a supporting base plate (1), an elution extraction component (2), a liquid storage barrel (5) and a centrifugal separation component (7), characterized in that: The elution and extraction component (2), the liquid storage barrel (5) and the centrifugal separation component (7) are all arranged on the supporting bottom plate (1); two liquid storage barrels (5) are symmetrically arranged; the output end of the elution and extraction component (2) is located directly above one of the liquid storage barrels (5); and the centrifugal separation component (7) is connected to one side of the other liquid storage barrel (5); The elution extraction component (2) comprises an irrigating liquid pumping tank (21) and an eluting liquid pumping tank (22), the irrigating liquid pumping tank (21) and the eluting liquid pumping tank (22) being arranged side by side, the bottom end of the irrigating liquid pumping tank (21) being connected to an irrigating liquid extraction pipe (25) and an irrigating liquid injection pipe (26), the bottom end of the eluting liquid pumping tank (22) being connected to an eluting liquid extraction pipe (27) and an eluting liquid delivery pipe (28), a three-way pipe (29) being connected between the irrigating liquid injection pipe (26) and the eluting liquid extraction pipe (27), the other interface of the three-way pipe (29) being connected to an eluting liquid injection pipe (210), and each pipe connected to the bottom end of the irrigating liquid pumping tank (21) and the eluting liquid pumping tank (22) being provided with a one-way valve unit; A filter assembly (4) is installed on the body of the eluent delivery pipe (28), the filter assembly (4) comprising a fixed outer shell (42) and a movable outer shell (43), the movable outer shell (43) being movably connected to the bottom end of the fixed outer shell (42), and filter screen units are arranged inside the fixed outer shell (42) and the movable outer shell (43).

2. The bovine lung active substance elution and separation device according to claim 1, characterized in that: The four corners of the bottom of the support base plate (1) are fixedly connected to support base rods (11); a limit fixing plate (12) is arranged above the support base plate (1) in a parallel shape; the four corners of the limit fixing plate (12) are fixedly connected to limit support rods (13); the bottom ends of the limit support rods (13) are fixedly connected to the upper plate surface of the support base plate (1); and the tank bodies of the irrigation liquid pumping tank (21) and the eluent pumping tank (22) are both fixedly connected to the limit fixing plate (12).

3. The bovine lung active substance elution and separation device according to claim 2, characterized in that: A movable push rod (23) and a piston (24) are provided in both the irrigation liquid pumping tank (21) and the eluent pumping tank (22), and the movable push rod (23) is coaxially fixedly connected to the top of the piston (24).

4. The bovine lung active substance elution and separation device according to claim 3, characterized in that: A synchronous connection plate (215) is fixedly connected between the upper ends of the two movable push rods (23), and a hydraulic push rod (216) is provided between the irrigation fluid pumping tank (21) and the eluent pumping tank (22), wherein the bottom end of the hydraulic push rod (216) is fixedly connected to the limit fixing plate (12), and the movable upper end of the hydraulic push rod (216) is fixedly connected to the lower plate surface of the synchronous connection plate (215).

5. The bovine lung active substance elution and separation device according to claim 1, characterized in that: The one-way valve unit comprises an irrigating liquid extraction tube one-way valve (211), an irrigating liquid injection tube one-way valve (212), an eluent extraction tube one-way valve (213) and an eluent delivery tube one-way valve (214); the irrigating liquid extraction tube one-way valve (211) is fixedly connected in the irrigating liquid extraction tube (25), the irrigating liquid injection tube one-way valve (212) is fixedly connected in the irrigating liquid injection tube (26), the eluent extraction tube one-way valve (213) is fixedly connected in the eluent extraction tube (27), and the eluent delivery tube one-way valve (214) is fixedly connected in the eluent delivery tube (28); The upper plate surface of the support base plate (1) is fixedly connected to a lavage liquid storage tank (3), the lavage liquid extraction tube (25) is inserted into the lavage liquid storage tank (3), and the lavage liquid storage tank (3) stores lavage liquid.

6. The bovine lung active substance elution and separation device according to claim 1, characterized in that: A top cover (44) is movably mounted on the upper end of the fixed housing (42) of the filter assembly (4); the fixed housing (42) is connected to an eluent delivery pipe (28); and a filtrate discharge pipe (41) is connected to the bottom end of the movable housing (43) of the filter assembly (4); The liquid outlet of the filtrate discharge pipe (41) is located directly above one of the liquid storage barrels (5); An external threaded docking surface (46) is formed on the bottom outer wall of the fixed outer shell (42), and an internal threaded docking surface (47) is formed on the upper inner wall of the movable outer shell (43). The external threaded docking surface (46) is threadedly docked with the internal threaded docking surface (47).

7. The bovine lung active substance elution and separation device according to claim 6, characterized in that: The filter unit comprises a conical filter (45), a funnel-shaped filter (48) and a cylindrical filter (49); the conical filter (45) is fixedly connected to the center of the bottom surface of the top cover (44); the funnel-shaped filter (48) is fixedly connected to the upper opening of the movable housing (43); the cylindrical filter (49) is integrally connected below the funnel-shaped filter (48); and the conical filter (45) is placed directly above the cylindrical filter (49).

8. The bovine lung active substance elution and separation device according to claim 1, characterized in that: A rotating base plate (6) is movably arranged on the supporting base plate (1), and a rotating motor (61) is fixedly connected to the lower plate surface of the supporting base plate (1). The rotating shaft of the rotating motor (61) passes through the supporting base plate (1), and the upper end of the rotating shaft of the rotating motor (61) is fixedly connected to the center of the lower plate surface of the rotating base plate (6). A rotating bearing (62) is sleeved on the rotating shaft of the rotating motor (61) passing through the supporting base plate (1), and the two ends of the upper plate surface of the rotating base plate (6) are symmetrically connected to the barrel bottom limiting seats (63), and the bottom end of the liquid storage barrel (5) is clamped in the barrel bottom limiting seat (63).

9. The bovine lung active substance elution and separation device according to claim 8, characterized in that: The bottom surface of the barrel bottom limiting seat (63) is connected to a compression air bag (67), the inner wall of the barrel bottom limiting seat (63) is connected to an annular expansion air bag (69), an air guide channel (68) is provided in the barrel bottom limiting seat (63), and the compression air bag (67) and the annular expansion air bag (69) are connected via the air guide channel (68); A centrifugal connecting rod (64) is fixedly connected to the bottom center of the barrel bottom limiting seat (63); the centrifugal connecting rod (64) passes through the rotating bottom plate (6); a centrifugal bearing (66) is sleeved on the rod body of the centrifugal connecting rod (64) that passes through the rotating bottom plate (6); and a fixed friction plate (65) is coaxially fixedly connected to one end of the centrifugal connecting rod (64) located below the rotating bottom plate (6).

10. The bovine lung active substance elution and separation device according to claim 9, characterized in that: The centrifugal separation assembly (7) comprises a hydraulic outer rod (71) and a hydraulic inner rod (72), wherein the hydraulic inner rod (72) is movably inserted at both ends of the hydraulic outer rod (71), and the rod body of the hydraulic outer rod (71) is connected to the support base plate (1). The hydraulic inner rod (72) connected to the upper end of the hydraulic outer rod (71) is fixedly connected to a fixed clamping plate (73), and the lower plate surface of the fixed clamping plate (73) is movably connected to a barrel top positioning piston (75), and the barrel top positioning piston (75) is located directly above the liquid storage barrel (5). The hydraulic inner rod (72) connected to the lower end of the hydraulic outer rod (71) is fixedly connected to a centrifugal motor (74), and the rotating shaft of the centrifugal motor (74) passes through the support base plate (1). The upper end of the rotating shaft of the centrifugal motor (74) is coaxially fixedly connected to a movable friction plate (76), and the movable friction plate (76) is located directly below the fixed friction plate (65); A docking shaft (710) is coaxially connected between the fixed clamping plate (73) and the barrel top positioning piston (75); an upper annular groove (77) is formed on the lower plate surface of the fixed clamping plate (73); a lower annular groove (78) is formed on the upper end surface of the barrel top positioning piston (75); and a plurality of balls (79) are clamped between the upper annular groove (77) and the lower annular groove (78); The two ends of the rotating bottom plate (6) are fixedly connected to limit top blocks (610), and the hydraulic outer rod (71) of the centrifugal separation component (7) is fixedly connected to a limit switch (711) on the rod body on the side close to the rotating bottom plate (6).

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