Nucleic acid treatment method

Abstract

The embodiment of the invention provides a nucleic acid treatment method, and relates to the technical field of biomolecule detection. According to the nucleic acid treatment method, in the nucleic acid extraction process, the magnetic beads can be stirred, can be dispersed in liquid vortex, can suspend under the action of a magnetic field and cannot fall into other channels after being far away from the magnetic field, so that the loss of the magnetic beads is reduced, nucleic acid extraction can be completed, the detection accuracy is improved, and the detection efficiency is improved. High-precision nucleic acid extraction is achieved, and meanwhile high-precision nucleic acid amplification can be achieved.

Description

Technical Field

[0001] The present invention relates to the technical field of biomolecule detection, and in particular to a nucleic acid processing method. Background Art

[0002] Microfluidics, also known as chip labs, is a technology that manipulates fluids at the micrometer scale. This technology has miniaturized the basic functions of chemical and biological laboratories onto a chip that is only a few square centimeters in size.

[0003] Through the intersection of analytical chemistry, micro-electromechanical processing, computer, electronics, materials, biology, medicine and other disciplines, miniaturization, automation, integration and portability from sample processing to detection are achieved.

[0004] In the prior art, it is difficult to achieve high-precision nucleic acid extraction through microfluidic chips. Summary of the invention

[0005] The present invention provides a nucleic acid processing method, which can complete nucleic acid extraction, improve the accuracy of inspection, and realize high-precision nucleic acid extraction.

[0006] The embodiments of the present invention can be implemented as follows:

[0007] The present invention provides a method for processing nucleic acid, comprising:

[0008] S1: placing a microfluidic chip in an analyzer, wherein the microfluidic chip has an information extraction chamber and a first liquid storage chamber, a second liquid storage chamber, a third liquid storage chamber and a fourth liquid storage chamber connected to the information extraction chamber, the information extraction chamber contains a plurality of magnetic beads, and the first liquid storage chamber, the second liquid storage chamber, the third liquid storage chamber and the fourth liquid storage chamber contain a lysate, a first washing liquid, a second washing liquid and an eluent respectively;

[0009] S2: adding a sample into the information extraction chamber;

[0010] S3: transporting the lysate in the first liquid storage chamber to the information extraction chamber;

[0011] S4: controlling the microfluidic chip to rotate at a first preset speed for a first preset time;

[0012] S5: Control the magnetic steel magnetic part in the analyzer to be close to the microfluidic chip so that the magnetic beads are suspended in the information extraction chamber; wherein the method of step S5 also includes:

[0013] S501: Control the microfluidic chip to rotate in a forward direction at a fourth preset speed and then decelerate for a third preset time;

[0014] S502: Control the microfluidic chip to rotate in the reverse direction at a fourth preset speed and then decelerate for a third preset time;

[0015] S503: repeating steps S501-S502 to generate a vortex in the liquid in the information extraction chamber;

[0016] S6: controlling the rotation speed of the microfluidic chip to run at a fifth preset speed, so that the magnetic beads gather toward the side wall in the information extraction chamber;

[0017] S7: controlling the magnetic steel magnetic member to rise and move away from the microfluidic chip, so that the magnetic beads stop suspending;

[0018] S8: heating the temperature-controlled air cavity so that the air in the temperature-controlled air cavity expands and enters the information extraction chamber, so that the gas in the temperature-controlled air cavity is discharged, wherein the microfluidic chip further has a temperature-controlled air cavity, and the temperature-controlled air cavity is connected to the information extraction chamber;

[0019] S9: Refrigerating the temperature-controlled air cavity so that the temperature-controlled air cavity is in a negative pressure state, so as to absorb the liquid in the information extraction chamber into the temperature-controlled air cavity, so that the magnetic beads and the nucleic acids adsorbed by the magnetic beads remain in the information extraction chamber;

[0020] S10: adding the first detergent in the second liquid storage chamber into the information extraction chamber, and repeating steps S4-S9;

[0021] S11: adding the second detergent in the third liquid storage chamber into the information extraction chamber, and repeating steps S4-S7;

[0022] S12: Add the elution solution in the fourth liquid storage chamber into the information extraction chamber, and repeat steps S4-S7 to detach the nucleic acid from the magnetic beads.

[0023] In an optional embodiment, after step S12, the nucleic acid processing method further comprises:

[0024] S13: heating the temperature control chamber so that the air in the temperature control chamber expands and enters the information extraction chamber, so that the gas in the temperature control chamber is discharged, wherein the microfluidic chip further comprises a detection chamber and a temperature control chamber, and the information extraction chamber, the detection chamber and the temperature control chamber are sequentially connected;

[0025] S14: Refrigerating the temperature control chamber so that the temperature control chamber is in a negative pressure state, so as to deliver the eluate containing nucleic acid in the information extraction chamber to the detection chamber;

[0026] S15: Heat the detection chamber at a preset temperature to achieve nucleic acid amplification.

[0027] In an optional implementation, step S14 includes:

[0028] S141: Refrigerating the temperature control chamber so that the temperature control chamber is in a negative pressure state;

[0029] S142: controlling the microfluidic chip to rotate at a speed greater than a second preset speed, so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugation;

[0030] Alternatively, the microfluidic chip is controlled to rotate at a third preset speed for a second preset time, and then the microfluidic chip is controlled to rotate at the second preset speed for the second preset time, and then the chip is controlled to rotate at the third preset speed for the second preset time, thereby performing a cyclic control so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugation, and the third preset speed is less than the second preset speed.

[0031] In an optional implementation, the second preset time is 0-10s, the second preset speed is 600-6000rpm, and the third preset speed is 0-1000rpm.

[0032] In an optional embodiment, the preset temperature condition is 60-95°C.

[0033] In an optional implementation, the first preset speed is 100-6000 rpm, and the first preset time is 1-700 s.

[0034] In an optional embodiment, the diameter of the magnetic beads is 200-5000 nm, and the fourth preset speed is 200-3000 rpm.

[0035] In an optional implementation, the method of step S5 further includes:

[0036] The temperature of the information extraction chamber is controlled to be above 37°C.

[0037] In an optional embodiment, the diameter of the magnetic beads is 100 to 5000 nm.

[0038] In an optional implementation, the fifth preset speed is 100 rpm to 900 rpm.

[0039] The beneficial effects of the nucleic acid processing method of the embodiment of the present invention include, for example:

[0040] In an embodiment of the present invention, a nucleic acid processing method is provided, which comprises: S1: placing a microfluidic chip in an analyzer; S2: adding a sample to an information extraction chamber; S3: transporting a lysate in a first liquid storage chamber to the information extraction chamber; S4: controlling the microfluidic chip to rotate at a first preset speed for a first preset time; S5: controlling a magnetic steel magnetic part in the analyzer to be close to the microfluidic chip so that magnetic beads are suspended in the information extraction chamber; S6: controlling the rotation speed of the microfluidic chip to run at a fifth preset speed so that the magnetic beads are suspended in the information extraction chamber; The beads gather toward the side wall in the information extraction chamber; S7: control the magnetic steel magnetic part to rise and move away from the microfluidic chip, so that the magnetic beads stop floating; S8: heat the temperature-controlled air cavity, so that the air in the temperature-controlled air cavity expands and enters the information extraction chamber, so that the gas in the temperature-controlled air cavity is discharged, wherein the microfluidic chip also has a temperature-controlled air cavity, and the temperature-controlled air cavity is connected to the information extraction chamber; S9: cool the temperature-controlled air cavity, so that the temperature-controlled air cavity is in a negative pressure state, so as to suck the liquid in the information extraction chamber into the temperature-controlled air cavity, so that the magnetic beads remain in the information extraction chamber; S10: put the second The first detergent in the liquid storage chamber is added to the information extraction chamber, and steps S4-S9 are repeated; S11: the second detergent in the third liquid storage chamber is added to the information extraction chamber, and steps S4-S9 are repeated; S12: the eluent in the fourth liquid storage chamber is added to the information extraction chamber, and steps S4-S7 are repeated to separate the nucleic acid on the magnetic beads, wherein the method of step S5 also includes: S501: controlling the microfluidic chip to rotate in a forward direction to a fourth preset speed and then decelerate for a third preset time; S502: controlling the microfluidic chip to rotate in a forward direction to a fourth preset speed and then decelerate for a third preset time; The fluidic chip moves for a third preset time in a manner of accelerating in the reverse direction to a fourth preset speed and then decelerating; S503: repeating steps S501-S502 to generate a vortex in the liquid in the information extraction chamber. During the nucleic acid extraction process, the nucleic acid processing method can stir the magnetic beads, and the magnetic beads can be dispersed in the liquid vortex. The magnetic beads can be suspended under the action of the magnetic field, and can be away from the magnetic field without falling into other channels, thereby reducing the loss of magnetic beads. It can complete the extraction of nucleic acids, improve the accuracy of inspection, and achieve high-precision nucleic acid extraction. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show certain embodiments of the present invention and therefore should not be regarded as limiting the scope. For ordinary technicians in this field, other related drawings can be obtained based on these drawings without paying creative work.

[0042] Figure 1 Schematic diagram of a microfluidic chip provided in an embodiment of the present invention.

[0043] Icons: 10 microfluidic chip; 11 chip body; 12 information extraction chamber; 121 bottom wall; 122 side wall; 13 temperature-controlled air cavity; 14 siphon; 15 rotation center; 16 detection chamber; 161-quantification pool; 162-detection pool; 17 temperature control chamber; 18-first liquid storage chamber; 19-second liquid storage chamber; 20-third liquid storage chamber; 21-fourth liquid storage chamber. DETAILED DESCRIPTION

[0044] In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0045] Therefore, the following detailed description of the embodiments of the present invention provided in the accompanying drawings is not intended to limit the scope of the invention claimed for protection, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0046] It should be noted that similar reference numerals and letters denote similar items in the following drawings, and therefore, once an item is defined in one drawing, further definition and explanation thereof is not required in subsequent drawings.

[0047] In the description of the present invention, it should be noted that if the terms "upper", "lower", "inside", "outside", etc. appear to indicate an orientation or position relationship, they are based on the orientation or position relationship shown in the accompanying drawings, or are the orientation or position relationship in which the product of the invention is usually placed when used. They are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0048] In addition, the terms “first”, “second”, etc., if used, are merely used to distinguish between the descriptions and should not be understood as indicating or implying relative importance.

[0049] It should be noted that, in the absence of conflict, the features in the embodiments of the present invention may be combined with each other.

[0050] Microfluidic chips have the characteristics of flexible combination of multiple unit technologies and large-scale integration, so that a large amount of information can be obtained with a small amount of samples, and it is more likely to go beyond a single analysis function and become an overall micro-multiple operation platform. Since microfluidic chips also have the advantages of small size, low reagent consumption, fast analysis speed, and easy integration, they provide broad prospects for their application in many fields such as biomedical research, drug synthesis screening, environmental monitoring, and health quarantine.

[0051] As mentioned in the background technology, microfluidic chips, also known as chip labs, are a technology for manipulating fluids at the micrometer scale. This technology miniaturizes the basic functions of chemical and biological laboratories onto a chip that is only a few square centimeters in size. Through the intersection of analytical chemistry, micro-electromechanical processing, computers, electronics, materials, biology, medicine and other disciplines, miniaturization, automation, integration and portability from sample processing to detection are achieved.

[0052] In the prior art, it is difficult to achieve high-precision nucleic acid extraction and amplification through microfluidic chips. Specifically, although patent CN116371491A provides a microfluidic chip, there is no suitable control method to achieve high-precision nucleic acid extraction and amplification.

[0053] In view of this, please refer to Figure 1 The nucleic acid processing method provided in the embodiments of the present invention can solve this problem, which will be described in detail below.

[0054] An embodiment of the present invention provides a method for processing nucleic acid, which includes:

[0055] S1: Place the microfluidic chip 10 in the analyzer, wherein the microfluidic chip has an information extraction chamber 12 and a first liquid storage chamber 18, a second liquid storage chamber 19, a third liquid storage chamber 20 and a fourth liquid storage chamber 21 connected to the information extraction chamber 12, a plurality of magnetic beads (the diameter of the magnetic beads can be 100 to 5000 nm) are contained in the information extraction, and the first liquid storage chamber 18, the second liquid storage chamber 19, the third liquid storage chamber 20 and the fourth liquid storage chamber 21 contain a lysis solution, a first washing solution, a second washing solution and an elution solution, respectively.

[0056] It should be noted that, in this embodiment, the microfluidic chip can be a microfluidic chip in the prior art, which has a chip body 11 and an information extraction chamber 12, a first liquid storage chamber 18, a second liquid storage chamber 19, a third liquid storage chamber 20 and a fourth liquid storage chamber 21 arranged in the chip body. The chip body also has a rotation center 15, and the chip body 11 can rotate around the rotation center 15.

[0057] The microfluidic chip also has a temperature-controlled air cavity 13 disposed in the chip body 11, and the temperature-controlled air cavity and the information extraction chamber 12 are connected via a siphon 14.

[0058] S2: adding samples into the information extraction chamber; specifically, the information extraction chamber is connected to a sample adding hole, through which the sample is dripped or injected.

[0059] S3: The lysis solution in the first liquid storage chamber 18 is transported to the information extraction chamber, for example, the lysis solution is transported to the information extraction chamber 12 by squeezing.

[0060] S4: Control the microfluidic chip to rotate forward or reverse at a first preset speed (e.g., the first preset speed is 100-6000 rpm) for a first preset time (e.g., the first preset time is 1-700 s) so that the lysis liquid and the sample enter the bottom wall 121 of the information extraction chamber 12. The forward rotation here can be understood as Figure 1 The microfluidic chip rotates clockwise, and the reverse rotation can be understood as the microfluidic chip rotating counterclockwise.

[0061] S5: Control the magnetic parts (such as magnets) in the analyzer to be close to the microfluidic chip so that the magnetic beads are suspended in the information extraction chamber. The process of the magnets approaching the microfluidic chip here can be understood as the position of the microfluidic chip remains unchanged, the magnets descend in height, and the magnetic field generated by the magnets acts on the magnetic beads to make them suspended.

[0062] It should be noted that in step S5, trehalose is mixed on the outside of the magnetic beads, and the mixture is in a dried state. Under the action of the magnetic field, the magnetic beads can be quickly separated from the sugar, so that the magnetic beads can quickly capture the nucleic acid.

[0063] The method of step S5 further includes:

[0064] S501: Control the microfluidic chip to rotate in a forward direction in a manner of accelerating to a fourth preset speed (e.g., 600 rpm) and then decelerating for a third preset time (e.g., 1 s);

[0065] S502: Control the microfluidic chip to rotate in the reverse direction at a fourth preset speed and then decelerate for a third preset time;

[0066] S503: Repeat steps S501-S502 to generate a vortex in the liquid in the information extraction chamber.

[0067] Since the position of the magnet remains unchanged, the distance between the magnetic beads and the magnet will change during the rotation of the microfluidic chip, so the magnetic beads will move up and down. At the same time, the rotation of the microfluidic chip will continuously accelerate and decelerate while continuously reversing forward and reverse, so that the liquid is in a vortex. Finally, the magnetic beads are dispersed in the vortex.

[0068] The liquid in the information extraction chamber generates a vortex, which can stir the magnetic beads and disperse them in the liquid vortex. In this way, the magnetic beads can be suspended under the action of the magnetic field and can be away from the magnetic field without falling into other channels (the other channels here can be understood as chambers connected to the information extraction chamber), thereby reducing the loss of magnetic beads. It can complete the extraction of nucleic acids, improve the accuracy of inspection, and achieve high-precision nucleic acid extraction.

[0069] It should be noted that, in some embodiments, when the diameter of the magnetic beads is 200-5000 nm, the fourth preset speed is 200-3000 rpm.

[0070] In addition, the method of step S5 also includes: controlling the temperature of the information extraction chamber to rise to above 37° C., which can be understood as controlling the temperature of the information extraction chamber to rise to above 37° C. during the stirring process of the magnetic beads to improve the lysis efficiency.

[0071] S6: Control the rotation speed of the microfluidic chip to run at a fifth preset speed, the fifth preset speed is 100rpm-900rpm, for example, the fifth preset speed can be 100rpm or 500rpm or 900rpm, so that the magnetic beads gather toward the two opposite side walls 122 in the information extraction chamber. Generally speaking, the rotation direction of the microfluidic chip is different, and the side walls 122 where the magnetic beads gather are different. This can prevent the magnetic beads from falling into the channel opening of the information extraction chamber used to connect to the temperature-controlled air cavity, thereby preventing the magnetic beads from being lost.

[0072] S7: Control the magnetic part to rise and move away from the microfluidic chip to stop the magnetic beads from floating. Specifically, the magnetic part can be raised by controlling the magnetic field, for example, the magnetic part and the microfluidic chip are 5 mm apart. After the magnetic beads stop floating, they fall to the bottom of the information extraction chamber and gather (the bottom here is understood to be the bottom of the information extraction chamber along the direction of the rotation axis of the microfluidic chip).

[0073] S8: heating the temperature-controlled air cavity so that the air in the temperature-controlled air cavity expands and enters the information extraction chamber, and the gas in the temperature-controlled air cavity is discharged;

[0074] S9: Refrigerating the temperature-controlled air cavity so that the temperature-controlled air cavity is in a negative pressure state, so as to absorb the liquid in the information extraction chamber into the temperature-controlled air cavity, so that the magnetic beads and the nucleic acid adsorbed by the magnetic beads remain in the information extraction chamber;

[0075] S10: adding the first detergent in the second liquid storage chamber 19 into the information extraction chamber, and repeating steps S4-S9;

[0076] S11: adding the second detergent in the third liquid storage chamber 20 into the information extraction chamber, and repeating steps S4-S9;

[0077] S12: Add the elution solution in the fourth liquid storage chamber 21 into the information extraction chamber, and repeat steps S4-S7 to detach the nucleic acid from the magnetic beads.

[0078] In order to amplify the nucleic acid, after step S12, the nucleic acid processing method further includes:

[0079] S13: Heat the temperature control chamber 17 so that the air in the temperature control chamber expands and enters the information extraction chamber to discharge the gas in the temperature control chamber. The microfluidic chip also has a detection chamber 16 arranged on the chip body and a temperature control chamber 17 arranged on the chip body. It should be noted that the detection chamber 16 includes a quantitative pool 161 and a detection pool 162 which are connected to each other. The information extraction chamber, the quantitative pool 161 of the detection chamber 16 and the temperature control chamber are connected in sequence.

[0080] After the liquid (eluent containing nucleic acid) enters the detection chamber 16, the quantitative pool 161 plays the role of quantitatively dividing the liquid, and then transporting it to the detection pool 162, so that the detection pool 162 is just full and no bubbles appear. S14: Refrigerate the temperature control chamber so that the temperature control chamber 17 is in a negative pressure state, so as to deliver the eluent containing nucleic acid in the information extraction chamber 12 to the detection chamber 16, and the magnetic beads remain in the information extraction chamber 12. Specifically, when the eluent containing nucleic acid passes through the detection chamber 16, since the microfluidic chip is driven to rotate, the centrifugal force can disperse the eluent containing nucleic acid into different channels of the detection chamber (the different channels here can be understood as different quantitative pools 161 of the detection chamber 16 and the channel chamber of a detection chamber adjacent to the temperature control chamber 17), and the excess eluent will enter the temperature control chamber for storage. It can be seen that the temperature control chamber 17 plays the role of a pump and waste liquid storage.

[0081] It should be noted that Figure 1 As shown, the channel chamber volume of a detection chamber adjacent to the temperature control chamber 17 is larger than that of other channels. At the same time, the channel chamber adjacent to the temperature control chamber can act as a waste liquid pool, and the side wall interface connecting the temperature control chamber and the channel chamber is relatively close to the rotation center.

[0082] In other embodiments, the channel chamber may be integrated with the temperature control chamber, and the side wall interface connecting the temperature control chamber and the channel chamber may be arranged in the middle of the side wall of the temperature control chamber.

[0083] S15: Heat the detection chamber 16 at a preset temperature condition (eg, the preset temperature condition is 60-95° C.) to achieve nucleic acid amplification.

[0084] It should be noted that in this embodiment, the preset temperature condition is a series of temperature cycles, for example: it can first reach 60° and last for 5 seconds; then heat to 72° and keep for 10 seconds; then heat to 95° and keep for 15 seconds; then return to 60°, and cycle in this way.

[0085] In this embodiment, step S14 includes:

[0086] S141: Refrigerate the temperature control room so that the temperature control room is in a negative pressure state;

[0087] S142: When the flow channel (which can be understood as the communication channel between the quantitative pool 161 and the detection pool 162) is larger, for example, larger than 500 microns x 500 microns, the microfluidic chip is controlled to rotate at a uniform speed greater than the second preset speed, so that the liquid in the detection chamber passes through the quantitative pool 161 under the action of centrifugation and enters the detection pool 162 of the detection chamber;

[0088] Alternatively, in the case of a smaller flow channel, such as a size less than 200 microns x 200 microns, the microfluidic chip is controlled to rotate at a third preset speed for a second preset time, and then the microfluidic chip is controlled to rotate at a second preset speed for a second preset time, and then the chip is controlled to rotate at the third preset speed for a second preset time, thereby performing a cyclic control so that the liquid in the detection chamber passes through the quantitative pool 161 under the action of centrifugation and enters the detection pool 162 of the detection chamber, and the third preset speed is less than the second preset speed.

[0089] Among them, the second preset time is 0-10s, the second preset speed is 600-6000rpm, and the third preset speed is 0-1000rpm.

[0090] In summary, the nucleic acid processing method can use nanoscale magnetic beads to stir and separate multiple samples, and achieve high-precision nucleic acid extraction and amplification through the control method of the microfluidic chip.

[0091] The above is only a specific embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed by the present invention should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention should be based on the protection scope of the claims.

Claims

1. A method for processing nucleic acid, characterized in that: The nucleic acid processing method includes: S1: placing a microfluidic chip in an analyzer, wherein the microfluidic chip has an information extraction chamber and a first liquid storage chamber, a second liquid storage chamber, a third liquid storage chamber and a fourth liquid storage chamber connected to the information extraction chamber, the information extraction chamber contains a plurality of magnetic beads, and the first liquid storage chamber, the second liquid storage chamber, the third liquid storage chamber and the fourth liquid storage chamber contain a lysate, a first washing liquid, a second washing liquid and an eluent respectively; S2: adding a sample into the information extraction chamber; S3: transporting the lysate in the first liquid storage chamber to the information extraction chamber; S4: controlling the microfluidic chip to rotate at a first preset speed for a first preset time; S5: controlling the magnetic component in the analyzer to be close to the microfluidic chip so that the magnetic beads are suspended in the information extraction chamber; wherein the method of step S5 further includes: S501: Control the microfluidic chip to rotate in a forward direction at a fourth preset speed and then decelerate for a third preset time; S502: Control the microfluidic chip to rotate in the reverse direction at a fourth preset speed and then decelerate for a third preset time; S503: repeating steps S501-S502 to generate a vortex in the liquid in the information extraction chamber; S6: controlling the rotation speed of the microfluidic chip to run at a fifth preset speed, so that the magnetic beads gather toward the side wall in the information extraction chamber; S7: controlling the magnetic member to rise and move away from the microfluidic chip, so that the magnetic beads stop suspending; S8: heating the temperature-controlled air cavity so that the air in the temperature-controlled air cavity expands and enters the information extraction chamber, so that the gas in the temperature-controlled air cavity is discharged, wherein the microfluidic chip further has a temperature-controlled air cavity, and the temperature-controlled air cavity is connected to the information extraction chamber; S9: Refrigerating the temperature-controlled air cavity so that the temperature-controlled air cavity is in a negative pressure state, so as to absorb the liquid in the information extraction chamber into the temperature-controlled air cavity, so that the magnetic beads and the nucleic acids adsorbed by the magnetic beads remain in the information extraction chamber; S10: adding the first detergent in the second liquid storage chamber into the information extraction chamber, and repeating steps S4-S9; S11: adding the second detergent in the third liquid storage chamber into the information extraction chamber, and repeating steps S4-S9; S12: Add the elution solution in the fourth liquid storage chamber into the information extraction chamber, and repeat steps S4-S7 to detach the nucleic acid from the magnetic beads.

2. The method for treating nucleic acid according to claim 1, wherein: After step S12, the nucleic acid processing method further includes: S13: heating the temperature control chamber so that the air in the temperature control chamber expands and enters the information extraction chamber, so that the gas in the temperature control chamber is discharged, wherein the microfluidic chip further comprises a detection chamber and a temperature control chamber, and the information extraction chamber, the detection chamber and the temperature control chamber are sequentially connected; S14: Refrigerating the temperature control chamber so that the temperature control chamber is in a negative pressure state, so as to deliver the eluate containing nucleic acid in the information extraction chamber to the detection chamber; S15: Heating the detection chamber at a preset temperature to achieve nucleic acid amplification.

3. The method for treating nucleic acid according to claim 2, wherein: The step S14 comprises: S141: Refrigerating the temperature control chamber so that the temperature control chamber is in a negative pressure state; S142: controlling the microfluidic chip to rotate at a speed greater than a second preset speed, so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugation; Alternatively, the microfluidic chip is controlled to rotate at a third preset speed for a second preset time, and then the microfluidic chip is controlled to rotate at the second preset speed for the second preset time, and then the chip is controlled to rotate at the third preset speed for the second preset time, thereby performing a cyclic control so that the liquid in the detection chamber enters the detection pool of the detection chamber under the action of centrifugation, and the third preset speed is less than the second preset speed.

4. The method for treating nucleic acid according to claim 3, characterized in that: The second preset time is 0-10s, the second preset speed is 600-6000rpm, and the third preset speed is 0-1000rpm.

5. The method for treating nucleic acid according to claim 2, characterized in that: The preset temperature condition is 60-95°C.

6. The method for processing nucleic acid according to claim 1, characterized in that: The first preset speed is 100-6000 rpm, and the first preset time is 1-700 s.

7. The method for processing nucleic acid according to claim 1, characterized in that: The diameter of the magnetic beads is 200-5000 nm, and the fourth preset speed is 200-3000 rpm.

8. The method for processing nucleic acid according to claim 1, characterized in that: The method of step S5 further includes: The temperature of the information extraction chamber is controlled to be above 37°C.

9. The method for processing nucleic acid according to claim 1, characterized in that: The diameter of the magnetic beads is 100-5000 nm.

10. The method for processing nucleic acid according to claim 1, characterized in that: The fifth preset speed is 100 rpm to 900 rpm.

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