1ml pipette and control method thereof

Abstract

The present invention relates to a 1ml pipette and a control method thereof. The pipette comprises a tip head, a drive control board, a main pump body, an air pressure sensor, a tip head adapter for loading the tip head, a plunger unit slidably mounted in the main pump body, and a drive mechanism for driving the plunger unit to move relative to the main pump body. The tip head adapter is provided with a tip head detection mechanism for detecting whether the tip head is loaded thereon. The tip head detection mechanism comprises a follower, a tip head detection optical coupler, a reset elastic member for providing a reset elastic force to the follower, and a sleeve member sleeved around the loading head of the tip head adapter and movable relative to the loading head. After the tip head is loaded, the pipette can simultaneously detect whether the tip head is loaded, making it more convenient to use. The entire control method can better perform liquid aspiration and discharge, bringing convenience to use.

Description

Technical Field

[0001] The present invention relates to the technical field of pipettes, in particular to a 1ml pipette and a control method thereof. Background Art

[0002] A pipette, also known as a pipette gun, is a measuring tool used to transfer liquid from one container to another within a certain range. It is widely used in biology, chemistry and other fields. For example, when testing and analyzing blood or urine samples in the laboratory of a hospital, a pipette is usually needed to transfer the sample or the corresponding chemical testing aid into the corresponding vessel for testing.

[0003] Conventional pneumatic pipettes usually consist of a pipette body and a TIP head (also called a suction tip). The pipette body is provided with a gun tip used in conjunction with the TIP head. When pipetting is required, the Z-axis drive mechanism of the pipette system first drives the pipette body to move, so that the gun tip of the pipette body extends into the interior of the TIP head to complete the TIP head piercing action. Repeating the piercing action allows the pipette to replace the TIP head and perform subsequent aspiration actions.

[0004] After the existing pipette body is loaded with the TIP head, it is necessary to manually or visually check whether the loading is successful, which greatly affects the subsequent user experience.

[0005] Therefore, in the patent application of the present invention, the applicant has carefully studied a 1ml pipette and a control method thereof to solve the above problems. Summary of the Invention

[0006] The present invention aims to overcome the shortcomings of the above-mentioned prior art. Its main purpose is to provide a 1ml pipette and a control method thereof. After the TIP head is loaded, it can simultaneously detect whether the TIP is loaded, which is more convenient to use. The entire control method can better aspirate and discharge liquid, bringing convenience to use.

[0007] To achieve the above-mentioned purpose, the present invention adopts the following technical solutions:

[0008] A 1ml pipette comprising a tip, a drive control board, a main pump body, an air pressure sensor, a tip adapter for loading the tip, a plunger unit slidably mounted in the main pump body, and a drive mechanism for driving the plunger unit to move relative to the main pump body;

[0009] The TIP head is tightly connected to the loading head of the TIP adapter;

[0010] The TIP head has a first inner cavity, the TIP adapter has a second inner cavity, and the main pump body has a third inner cavity, and the first inner cavity is connected to the third inner cavity through the second inner cavity;

[0011] The air pressure sensor has a first detection port for communicating with the third inner cavity and detecting the air pressure in the third inner cavity and a second detection port for communicating with the external environment and performing detection;

[0012] The drive control board is provided with a main control circuit, a drive circuit for driving the drive mechanism, and a zero position detection optical coupler for detecting whether the plunger unit has returned to the zero position;

[0013] The main control circuit is respectively connected to the air pressure sensor, the drive circuit and the zero position detection optical coupler;

[0014] The TIP adapter is provided with a TIP head detection mechanism for detecting whether the TIP head is loaded thereon;

[0015] The TIP head detection mechanism includes a follower, a TIP detection optical coupler, a reset elastic member for providing a reset elastic force to the follower, and a sleeve member that is sleeved on the outer periphery of the loading head of the TIP adapter and can move relative to the loading head;

[0016] The follower is connected to the sleeve to move with the sleeve, and a first baffle is provided on the follower. The first baffle corresponds to the detection end of the TIP detection optical coupler, and the first baffle leaves or enters the detection end of the TIP detection optical coupler as the sleeve moves; the driving mechanism can also drive the connected follower to drive the follower to move relative to the main pump body;

[0017] The main control circuit is connected to the TIP detection optical coupler, a limited step portion is formed on the loading head of the TIP adapter, and the socket is located between the TIP head and the limited step portion;

[0018] After the TIP head is loaded onto the loading head of the TIP adapter, the sleeve moves inward until it is clamped between the TIP head and the limiting step. At this time, the tight fit force between the TIP head and the TIP adapter is greater than the restoring elastic force provided by the restoring elastic member to the follower, and the first blocking piece enters the detection end of the TIP detection optical coupler.

[0019] When the TIP head needs to be unloaded, the driving mechanism drives the follower to move outward, and the socket also moves outward along with the follower. At this time, the driving force of the driving mechanism to drive the follower to move is greater than the tight fit force between the TIP head and the TIP adapter, and the TIP head is squeezed to move outward. At the same time, the tight fit force between the TIP head and the TIP adapter disappears, and the follower continues to move outward and reset under the action of the reset elastic force. The first baffle leaves the detection end of the TIP detection optical coupler, and the socket continues to reset and move outward.

[0020] As a preferred solution, it further includes a bracket;

[0021] The TIP adapter, the drive control board, the main pump body and the drive mechanism are all arranged on the bracket, and the air pressure sensor, the zero position detection optical coupler and the TIP detection optical coupler are all arranged on the drive control board.

[0022] As a preferred solution, the bracket is a U-shaped bracket with an upper opening, and the drive control board covers the upper opening of the U-shaped bracket;

[0023] The U-shaped bracket includes a bottom plate and a front side plate and a rear side plate respectively connected to the front and rear sides of the upper end of the bottom plate, and the TIP adapter is arranged on the front side plate;

[0024] The main pump body is arranged on the bottom plate, and the front end surface of the main pump body abuts against the rear end surface of the front side plate, and the rear end surface of the front side plate is concavely provided with a truncated cone-shaped groove with a smaller front end and a larger rear end;

[0025] The front side plate has an inverted T-shaped groove inside, the first end of the inverted T-shaped groove is connected to the first detection port, the second end of the inverted T-shaped groove is connected to the second inner cavity, and the third end of the inverted T-shaped groove is connected to the third inner cavity through the truncated cone groove.

[0026] As a preferred solution, the zero position detection optocoupler and the TIP detection optocoupler are both arranged on the lower end surface of the drive control board, and the air pressure sensor is arranged on the upper end surface of the drive control board.

[0027] As a preferred solution, the upper end surface of the drive control board is also provided with a communication interface for connecting to a host computer or a PLC.

[0028] As a preferred solution, two ends of the reset elastic member are respectively connected to the follower and the main pump body, and the first baffle is arranged on the upper end surface of the follower.

[0029] As a preferred solution, the loading head of the TIP adapter is connected to the front end surface of the front side plate through a fixing block;

[0030] The fixing block has channels respectively communicating with the inverted T-shaped groove and the first inner cavity.

[0031] As a preferred solution, the follower is connected to the sleeve via a connecting rod;

[0032] A protrusion is provided on the upper end surface of the main pump body; the fixed block has a first through hole extending through it in the front-to-back direction, and the front side plate has a second through hole extending through it in the front-to-back direction. The front end of the connecting rod passes through the second through hole and the first through hole and is connected to the socket in sequence. The follower is arranged on the connecting rod and is located on the rear side of the front side plate. The other end of the connecting rod extends into the protrusion, and the two ends of the reset elastic member are respectively connected to the follower and the protrusion.

[0033] As a preferred solution, the protrusion has a third through hole extending through the protrusion in a front-to-rear direction, and the other end of the connecting rod extends into the third through hole and extends out of the protrusion.

[0034] A control method for a 1ml pipette, based on the 1ml pipette, comprises the following steps:

[0035] Step S1, connect the 1ml pipette to the host computer or PLC via RS485 or CAN communication, and determine whether the device ID needs to be modified. If the device ID needs to be modified, modify the device ID before entering step S2; otherwise, directly enter step S2;

[0036] Step S2, modifying the usage parameters;

[0037] Step S3, determining whether a new reset speed has been set in step S2, if so, controlling the drive mechanism to reset according to the set new reset speed, otherwise, controlling the drive mechanism to reset according to the default reset speed;

[0038] Step S4: Determine whether the TIP header is loaded. If so, proceed to step S5. Otherwise, first load the TIP header, then continue to determine whether the TIP header is loaded. Only after the TIP header is successfully loaded will proceed to step S5.

[0039] Step S5: determining whether new liquid level detection parameters have been set in step S2; if so, performing liquid level detection according to the set new liquid level detection parameters and controlling the drive mechanism to reset; otherwise, controlling the drive mechanism to reset according to the default reset speed;

[0040] The process proceeds to step S6 only after the liquid level is detected successfully, otherwise an alarm is issued and the process ends;

[0041] Step S6: First, perform aspiration and then discharge, and then determine whether there is a suction blockage, a discharge blockage, or insufficient aspiration and discharge precision. If there is a suction blockage or a discharge blockage, an alarm will be issued and the process ends; if there is insufficient aspiration and discharge precision, the aspiration and discharge volume will be adjusted by micro-increase pulse coefficient, and then the process proceeds to step S7; if there is no suction blockage, discharge blockage, or insufficient aspiration and discharge precision, the process proceeds directly to step S7;

[0042] Step S7: Unload the TIP header and end the control.

[0043] Compared with the prior art, the present invention has obvious advantages and beneficial effects. Specifically, it mainly has a TIP head detection mechanism, which can simultaneously detect whether the TIP is loaded after the TIP head is loaded, making it more convenient to use. The entire control method can better absorb and discharge liquid, bringing convenience to use.

[0044] To more clearly illustrate the structural features and effects of the present invention, it is described in detail below with reference to the accompanying drawings and specific embodiments. BRIEF DESCRIPTION OF THE DRAWINGS

[0045] Figure 1 2 is a schematic diagram of a three-dimensional assembly structure from a second angle of an embodiment of the present invention.

[0046] Figure 2 2 is a schematic diagram of a three-dimensional assembly structure from a second angle of an embodiment of the present invention.

[0047] Figure 3 It is a front view of an embodiment of the present invention (protective cover is not shown).

[0048] Figure 4 2 is a schematic diagram of the three-dimensional assembly structure of an embodiment of the present invention from a third angle (protective cover not shown).

[0049] Figure 5 It is a schematic diagram of the exploded structure of an embodiment of the present invention (protective cover is not shown).

[0050] Figure 6 1 is a schematic diagram of a first cross-sectional structure of an embodiment of the present invention (protective cover is not shown).

[0051] Figure 7 2 is a schematic diagram of a second cross-sectional structure of an embodiment of the present invention (protective cover not shown).

[0052] Figure 8 It is a general principle block diagram of an embodiment of the present invention.

[0053] Description of Figure Numbers:

[0054] TIP head 10, first inner cavity 11, protective cover 20, bracket 30, bottom plate 31, front side plate 32, truncated cone groove 321, inverted T-shaped groove 322, second through hole 323, rear side plate 33, drive control board 40, air pressure sensor 41, first detection port 411, second detection port 412, main control circuit 42, RGB indicator circuit 43, drive circuit 44, zero position detection optical coupler 45, communication interface 46, main pump body 50, third inner cavity 51, bump 5 2. Third through hole 521, TIP adapter 60, loading head 61, limiting step 611, second inner cavity 62, fixing block 63, channel 631, first through hole 632, plunger unit 70, drive mechanism 80, drive motor 81, slide rail 82, slider 83, lead screw 84, threaded sleeve 85, TIP head detection mechanism 90, follower 91, first baffle 911, TIP detection optical coupler 92, reset elastic member 93, socket 94, connecting rod 95. DETAILED DESCRIPTION

[0055] The present invention will be further described below with reference to the accompanying drawings and specific embodiments.

[0056] like Figures 1 to 8 As shown, a 1 ml pipette includes a TIP tip 10, a protective cover 20, a bracket 30, a drive control board 40, a main pump body 50, an air pressure sensor 41, a TIP adapter 60 for loading the TIP tip 10, a plunger unit 70 slidably mounted in the main pump body 50, and a drive mechanism 80 for driving the plunger unit 70 to move relative to the main pump body 50.

[0057] The TIP adapter 60 , the drive control board 40 , the main pump body 50 and the drive mechanism 80 are all disposed on the bracket 30 .

[0058] The TIP head 10 is tightly connected to the loading head 61 of the TIP adapter 60;

[0059] The TIP head 10 has a first inner cavity 11, the TIP adapter 60 has a second inner cavity 62, and the main pump body 50 has a third inner cavity 51. The first inner cavity 11 is connected to the third inner cavity 51 through the second inner cavity 62.

[0060] The air pressure sensor 41 has a first detection port 411 for communicating with the third inner cavity 51 and detecting the air pressure in the third inner cavity 51 and a second detection port 412 for communicating with the external environment and performing detection;

[0061] The driving control board 40 is provided with a main control circuit 42, an RGB indicator light circuit 43, a driving circuit 44 for driving the driving mechanism 80, and a zero position detection optical coupler 45 for detecting whether the plunger unit 70 has returned to the zero position;

[0062] The main control circuit 42 is connected to the air pressure sensor 41 , the RGB indicator light circuit 43 , the driving circuit 44 and the zero position detection optical coupler 45 respectively.

[0063] The drive mechanism can also drive the connected follower to move relative to the main pump body. The drive mechanism 80 includes a drive motor 81, a slide rail 82 disposed on the upper end surface of the base plate 31 described below, and a slider 83 slidably mounted on the slide rail 82. The drive motor 81 is a 28 mm stepper motor with a lead screw 84 fixedly mounted on its output end. The extended end of the lead screw 84 is threadedly mounted with a threaded sleeve 85, which is connected to the slider 83. One end of the plunger unit 70 extends into the third inner cavity 51 and moves relative to the main pump body 50. The other end of the plunger unit 70 is connected to the threaded sleeve 85.

[0064] The air pressure sensor 41 and the zero position detection optical coupler 45 are both provided on the drive control board 40. Preferably, the zero position detection optical coupler 45 is provided on the lower end surface of the drive control board 40, and the air pressure sensor 41 is provided on the upper end surface of the drive control board 40.

[0065] The upper end surface of the drive control board 40 is also provided with a communication interface 46 for connecting to a host computer or PLC. The communication interface 46 can be an RS485 interface or a CAN communication port. This embodiment supports both RS485 and CAN communication modes. The host computer or PLC sends a command sequence data frame consisting of the device ID, control instructions, and parameters to be set to this embodiment via the RS485 interface or CAN communication port. After receiving the command, the program in this embodiment automatically decodes it and executes the corresponding action.

[0066] The TIP adapter 60 is provided with a TIP head detection mechanism 90 for detecting whether the TIP head 10 is loaded thereon;

[0067] The TIP head detection mechanism 90 includes a follower 91, a TIP detection optical coupler 92, a reset elastic member 93 for providing a reset elastic force to the follower 91, and a sleeve 94 that is sleeved on the outer periphery of the loading head 61 of the TIP adapter 60 and can move relative to the loading head 61;

[0068] The follower 91 is connected to the sleeve 94 to move with the sleeve 94. Preferably, the follower 91 is connected to the sleeve 94 through a connecting rod 95.

[0069] The follower 91 is provided with a first baffle 911, which corresponds to the detection end of the TIP detection optical coupler 92. The first baffle 911 leaves or enters the detection end of the TIP detection optical coupler 92 as the socket 94 moves.

[0070] The main control circuit 42 is connected to a TIP detection optical coupler 92 , which is provided on the drive control board 40 . Preferably, the TIP detection optical coupler 92 is provided on the lower end surface of the drive control board 40 .

[0071] A limiting step portion 611 is formed on the loading head 61 of the TIP adapter 60 , and the socket 94 is located between the TIP head 10 and the limiting step portion 611 ;

[0072] After the TIP head 10 is loaded onto the loading head 61 of the TIP adapter 60, the sleeve 94 moves inward until it is sandwiched between the TIP head 10 and the limiting step 611. At this time, the tight fit force between the TIP head 10 and the TIP adapter 60 is greater than the restoring elastic force provided by the restoring elastic member 93 to the follower 91, and the first stopper 911 enters the detection end of the TIP detection optical coupler 92.

[0073] When the TIP head 10 needs to be unloaded, the driving mechanism 80 drives the follower 91 to move outward, and the socket 94 also moves outward along with the follower 91. At this time, the driving force of the driving mechanism 80 to drive the follower 91 to move is greater than the tight fit force between the TIP head 10 and the TIP adapter 60, and the TIP head 10 is squeezed outward. At the same time, the tight fit force between the TIP head 10 and the TIP adapter 60 disappears, and the follower 91 continues to move outward and reset under the action of the reset elastic force. The first blocking piece 911 leaves the detection end of the TIP detection optical coupler 92, and the socket 94 continues to reset outward.

[0074] Specifically, when the TIP head 10 needs to be unloaded, the driving motor 81 drives the slider 83 to move toward the plunger unit 70 until it abuts against the other end of the connecting rod 95. The slider 83 pushes the connecting rod 95 outward, and then the follower 91 and the socket 94 also move outward.

[0075] At this time, the driving force of the drive mechanism 80 driving the follower 91 to move is equivalent to the squeezing force of the socket on the TIP head. Since the driving force of the drive mechanism 80 driving the follower 91 to move is greater than the tight fit force between the TIP head 10 and the TIP adapter 60, the TIP head 10 is squeezed outward and separated from the TIP adapter 60 (i.e., the TIP head 10 is unloaded). At this time, the drive motor 81 stops operating, and the follower 91 continues to move outward and reset under the action of the reset elastic force. The first blocking piece 911 leaves the detection end of the TIP detection optical coupler 92, and the socket 94 continues to reset outward.

[0076] In this embodiment, the bracket 30 is a U-shaped bracket with an upper opening, and the drive control board 40 covers the upper opening of the U-shaped bracket;

[0077] The U-shaped bracket includes a bottom plate 31 and a front side plate 32 and a rear side plate 33 respectively connected to the front and rear sides of the upper end of the bottom plate 31.

[0078] The TIP adapter 60 is disposed on the front side plate 32. Preferably, the loading head 61 of the TIP adapter 60 is connected to the front end surface of the front side plate 32 via a fixing block 63. The fixing block 63 has a channel 631 that connects the inverted T-slot 322 and the first inner cavity 11.

[0079] The main pump body 50 is disposed on the bottom plate 31 and the front end surface of the main pump body 50 abuts against the rear end surface of the front side plate 32. The rear end surface of the front side plate 32 is concavely provided with a truncated cone-shaped groove 321 with a smaller front end and a larger rear end.

[0080] The front side plate 32 has an inverted T-shaped slot 322 inside, the first end of the inverted T-shaped slot 322 is connected to the first detection port 411, the second end of the inverted T-shaped slot 322 is connected to the second inner cavity 62, and the third end of the inverted T-shaped slot 322 is connected to the third inner cavity 51 through the truncated cone slot 321.

[0081] Two ends of the resetting elastic member 93 are respectively connected to the follower 91 and the main pump body 50 , and the first blocking piece 911 is provided on the upper end surface of the follower 91 .

[0082] A protrusion 52 is convexly provided on the upper end surface of the main pump body 50; the fixed block 63 has a first through hole 632 extending therethrough in the front-to-back direction, and the front side plate 32 has a second through hole 323 extending therethrough in the front-to-back direction. The front end of the connecting rod 95 passes through the second through hole 323 and the first through hole 632 and is connected to the socket 94 in sequence, and the follower 91 is arranged on the connecting rod 95 and is located on the rear side of the front side plate 32. The other end of the connecting rod 95 extends into the protrusion 52, and the two ends of the reset elastic member 93 are respectively connected to the follower 91 and the protrusion 52.

[0083] The protrusion 52 has a third through hole 521 extending therethrough in a front-to-back direction. The other end of the connecting rod 95 extends into the third through hole 521 and extends out of the protrusion 52 .

[0084] A control method for a 1ml pipette, based on the 1ml pipette, comprises the following steps:

[0085] Step S1, connect the 1ml pipette to the host computer or PLC via RS485 or CAN communication, and determine whether the device ID needs to be modified. If the device ID needs to be modified, modify the device ID before entering step S2; otherwise, directly enter step S2;

[0086] Step S2, modifying the usage parameters;

[0087] Step S3, determining whether a new reset speed has been set in step S2, if so, controlling the drive mechanism 80 to reset according to the set new reset speed, otherwise, controlling the drive mechanism 80 to reset according to the default reset speed;

[0088] Step S4: Determine whether the TIP header 10 is loaded. If so, proceed to step S5. Otherwise, first load the TIP header 10, then continue to determine whether the TIP header 10 is loaded, and proceed to step S5 only after the TIP header 10 is successfully loaded.

[0089] Step S5: Determine whether new liquid level detection parameters have been set in step S2. If so, perform liquid level detection according to the new liquid level detection parameters and control the drive mechanism 80 to reset. Otherwise, control the drive mechanism 80 to reset according to the default reset speed.

[0090] The process proceeds to step S6 only after the liquid level is detected successfully, otherwise an alarm is issued and the process ends;

[0091] Step S6: First, perform aspiration and then discharge, and then determine whether there is a suction blockage, a discharge blockage, or insufficient aspiration and discharge precision. If there is a suction blockage or a discharge blockage, an alarm will be issued and the process ends; if there is insufficient aspiration and discharge precision, the aspiration and discharge volume will be adjusted by micro-increase pulse coefficient, and then the process proceeds to step S7; if there is no suction blockage, discharge blockage, or insufficient aspiration and discharge precision, the process proceeds directly to step S7;

[0092] Step S7: Unload the TIP header 10 and end the control.

[0093] In this embodiment, if the reagent is extracted at a fixed position, the user can only move the reagent container to the TIP head 10 of this embodiment to perform the suction and discharge actions. This certainly cannot meet the user's flexible and convenient suction and discharge needs, so a Z-axis drive device is required to carry this embodiment to move up and down in the vertical direction. By controlling this Z-axis drive device, the lifting and lowering of this embodiment can be achieved (the forward and backward movements described in this embodiment are respectively changed to downward and upward movements), which facilitates the suction and discharge actions.

[0094] In this embodiment, the liquid level detection in step S5 includes air pressure liquid level detection and capacitance liquid level detection.

[0095] The description of liquid level detection is as follows:

[0096] After the TIP head 10 is loaded on the TIP adapter 60, the user uses the Z-direction drive device to drive this embodiment to move downward into the reagent container. Before it contacts the container, the entire air path is the same as the external atmospheric pressure. When the drive control board 40 sends an air pressure detection preparation, the drive motor of this embodiment drives the plunger unit 70 to retreat a certain distance. Then the drive control board 40 sends an air pressure detection instruction again. This embodiment will push the plunger unit 70 back to the zero position at a certain speed. When the TIP head 10 contacts the reagent, the liquid will enter the TIP head 10, causing the air pressure in the third inner cavity 51 to change. After the air pressure sensor 41 detects the change in the air pressure value, the corresponding air pressure value data will be transmitted to the main control circuit 42. The drive control board 40 calculates and compares the set air pressure detection threshold to determine whether the conditions for detecting the reagent liquid level are met. If they meet the requirements, the drive plunger unit 70 will be automatically stopped from moving, and it will be determined that the liquid level detection is successful. If the plunger unit 70 moves to the zero position and the air pressure value data output by the air pressure sensor 41 does not meet the standard of reaching the reagent liquid level, it will be judged that the liquid level detection has failed, and the output status is liquid level detection failure, and an alarm will be issued.

[0097] The description of liquid level detection is as follows:

[0098] Capacitive liquid level detection requires a prerequisite: the reagent has conductive properties. Through the conductive TIP head 10, a coaxial line is used to connect the TIP adapter 60 to the IPX terminal socket of the capacitance detection circuit of the drive control board 40. When this embodiment moves to the reagent surface, the conductive properties of the reagent will cause the capacitance value between the TIP head 10 and the capacitance detection circuit to change. The capacitance detection circuit will output the detected capacitance value to the main control circuit 42. After the main control circuit 42 calculates and compares it with a pre-set capacitance detection threshold, when the obtained capacitance value is greater than the set capacitance detection threshold, it is determined that the liquid level has been detected. When the obtained capacitance value is less than the pre-set capacitance detection threshold, it is determined that the liquid level has not been detected and an alarm is issued. At this point, the user can choose to continue detecting until detection is achieved or stop when the reagent remaining is insufficient.

[0099] In this embodiment, in step S6 , two states, namely, the blockage suction and the blockage discharge, are described as examples.

[0100] Define the external air pressure value as 2980, the air pressure value during liquid aspiration as 2965, and the air pressure value during liquid expulsion as 3000. Set the suction parameter, i.e. the lower limit of the air pressure value, to 2950, ​​and the discharge parameter, i.e. the upper limit of the air pressure value, to 3050.

[0101] 1) Description of the suction blockage problem: In actual use, the reagent sample or the solution to be tested may have clots or impurities. When the diameter of the clots or impurity particles is larger than the inner diameter of the suction hole of the TIP head 10, the clots or impurities will block the TIP head 10 during the liquid aspiration process in this embodiment, and the reagent or the solution to be tested cannot be aspirated into the TIP head 10. At this time, the present embodiment is still performing the liquid aspiration action, which is equivalent to the action of vacuuming in the third inner cavity 51. There will be a negative pressure in the air pressure sensor 41, and the actual air pressure value of the air pressure sensor 41 will be lower than the pressure value during liquid aspiration. By setting a lower limit of the air pressure value, that is, lower than the air pressure value during liquid aspiration, when the actually read air pressure value (for example: the actually read air pressure value is 2935) is lower than the set lower limit of the air pressure (2950), the software of the drive control board 40 automatically determines that it is a suction blockage based on calculation.

[0102] 2) Blockage problem description: In actual use, the user may have clots or impurities in the reagent sample or the solution to be tested. When the diameter of the clot or impurity particles is larger than the inner diameter of the suction hole of the TIP head 10, the clot or impurity may be sucked into the TIP head 10 at a certain angle. In the process of discharging liquid in this embodiment, the clot or impurity may turn over and block the TIP head 10, and the reagent or solution to be tested cannot be discharged from the TIP head 10. At this time, the embodiment is still performing the discharging action, which is equivalent to When the plunger unit 70 squeezes the gas in the third inner cavity 51, the pressure in the air pressure sensor 41 will increase, and the actual air pressure value of the air pressure sensor 41 will be lower than the pressure value when discharging liquid. By setting an upper limit of the air pressure value, that is, higher than the air pressure value when discharging liquid, the software of the drive control board 40 reads the air pressure value in the cavity in real time. When the actual read air pressure value (for example, the actual read air pressure value is 3070) is higher than the set upper limit of the air pressure (3050), the software of the drive control board 40 automatically determines that the drainage is blocked according to the calculation.

[0103] By setting the suction parameter, i.e., the lower limit of the air pressure value, and the discharge parameter, i.e., the upper limit of the air pressure value, the software of the drive control board 40 can automatically calculate and determine whether there is a blockage based on this setting. After that, the RGB indicator light circuit 43 will display red and status information prompts, so that the terminal user can know the operating status of this embodiment in real time.

[0104] The key points of the design of the present invention are: it mainly has a TIP head detection mechanism, which can simultaneously detect whether the TIP is loaded after the TIP head is loaded, making it more convenient to use. The entire control method can better absorb and discharge liquid, bringing convenience to use.

[0105] The above description is merely a preferred embodiment of the present invention and does not limit the technical scope of the present invention. Therefore, any minor modifications, equivalent changes and modifications made to the above embodiments based on the technical essence of the present invention are still within the scope of the technical solution of the present invention.

Claims

1. A 1ml pipette, characterized by: It includes a TIP head, a drive control board, a main pump body, an air pressure sensor, a TIP adapter for loading the TIP head, a plunger unit slidably mounted in the main pump body, and a drive mechanism for driving the plunger unit to move relative to the main pump body; The TIP head is tightly connected to the loading head of the TIP adapter; the TIP adapter, drive control board, main pump body and drive mechanism are all arranged on the bracket; The bracket is a U-shaped bracket with an upper opening, and the drive control board covers the upper opening of the U-shaped bracket; The U-shaped bracket includes a bottom plate and a front side plate and a rear side plate respectively connected to the front and rear sides of the upper end of the bottom plate, and the TIP adapter is arranged on the front side plate; The main pump body is arranged on the bottom plate, and the front end surface of the main pump body abuts against the rear end surface of the front side plate, and the rear end surface of the front side plate is concavely provided with a truncated cone-shaped groove with a smaller front end and a larger rear end; The front side plate has an inverted T-shaped groove inside, the first end of the inverted T-shaped groove is connected to the first detection port, the second end of the inverted T-shaped groove is connected to the second inner cavity, and the third end of the inverted T-shaped groove is connected to the third inner cavity through the truncated cone groove; The TIP head has a first inner cavity, the TIP adapter has a second inner cavity, and the main pump body has a third inner cavity, and the first inner cavity is connected to the third inner cavity through the second inner cavity; The air pressure sensor has a first detection port for communicating with the third inner cavity and detecting the air pressure in the third inner cavity and a second detection port for communicating with the external environment and performing detection; The drive control board is provided with a main control circuit, a drive circuit for driving the drive mechanism, and a zero position detection optical coupler for detecting whether the plunger unit has returned to the zero position; The main control circuit is respectively connected to the air pressure sensor, the drive circuit and the zero position detection optical coupler; The TIP adapter is provided with a TIP head detection mechanism for detecting whether the TIP head is loaded thereon; The TIP head detection mechanism includes a follower, a TIP detection optical coupler, a reset elastic member for providing a reset elastic force to the follower, and a sleeve member that is sleeved on the outer periphery of the loading head of the TIP adapter and can move relative to the loading head; The air pressure sensor, zero position detection optocoupler and TIP detection optocoupler are all arranged on the drive control board; the zero position detection optocoupler and TIP detection optocoupler are both arranged on the lower end surface of the drive control board, and the air pressure sensor is arranged on the upper end surface of the drive control board; the upper end surface of the drive control board is also provided with a communication interface for connecting to a host computer or PLC; The follower is connected to the sleeve to move with the sleeve, and a first baffle is provided on the follower. The first baffle corresponds to the detection end of the TIP detection optical coupler, and the first baffle leaves or enters the detection end of the TIP detection optical coupler as the sleeve moves; the driving mechanism can also drive the connected follower to drive the follower to move relative to the main pump body; The main control circuit is connected to the TIP detection optical coupler, a limited step portion is formed on the loading head of the TIP adapter, and the sleeve is located between the TIP head and the limited step portion; the two ends of the reset elastic member are respectively connected to the follower and the main pump body, and the first baffle is provided on the upper end surface of the follower; The loading head of the TIP adapter is connected to the front end surface of the front side plate through a fixing block; The fixing block has channels respectively communicating with the inverted T-shaped groove and the first inner cavity; After the TIP head is loaded onto the loading head of the TIP adapter, the sleeve moves inward until it is clamped between the TIP head and the limiting step. At this time, the tight fit force between the TIP head and the TIP adapter is greater than the restoring elastic force provided by the restoring elastic member to the follower, and the first blocking piece enters the detection end of the TIP detection optical coupler. When the TIP head needs to be unloaded, the driving mechanism drives the follower to move outward, and the socket also moves outward along with the follower. At this time, the driving force of the driving mechanism to drive the follower to move is greater than the tight fit force between the TIP head and the TIP adapter, and the TIP head is squeezed to move outward. At the same time, the tight fit force between the TIP head and the TIP adapter disappears, and the follower continues to move outward and reset under the action of the reset elastic force. The first baffle leaves the detection end of the TIP detection optical coupler, and the socket continues to reset and move outward.

2. The 1 ml pipette according to claim 1, characterized in that: The follower is connected to the sleeve member via a connecting rod; A protrusion is provided on the upper end surface of the main pump body; the fixed block has a first through hole extending through it in the front-to-back direction, and the front side plate has a second through hole extending through it in the front-to-back direction. The front end of the connecting rod passes through the second through hole and the first through hole and is connected to the socket in sequence. The follower is arranged on the connecting rod and is located on the rear side of the front side plate. The other end of the connecting rod extends into the protrusion, and the two ends of the reset elastic member are respectively connected to the follower and the protrusion.

3. The 1 ml pipette according to claim 2, characterized in that: The protrusion has a third through hole which penetrates along the front-to-back direction thereof, and the other end of the connecting rod extends into the third through hole and extends out of the protrusion.

4. A method for controlling a 1ml pipette, characterized in that: The 1 ml pipette according to any one of claims 1 to 3 is provided, wherein the control method comprises the following steps: Step S1, connect the 1ml pipette to the host computer or PLC via RS485 or CAN communication, and determine whether the device ID needs to be modified. If the device ID needs to be modified, modify the device ID before entering step S2; otherwise, directly enter step S2; Step S2, modifying the usage parameters; Step S3, determining whether a new reset speed has been set in step S2, if so, controlling the drive mechanism to reset according to the set new reset speed, otherwise, controlling the drive mechanism to reset according to the default reset speed; Step S4: Determine whether the TIP header is loaded. If so, proceed to step S5. Otherwise, first load the TIP header, then continue to determine whether the TIP header is loaded. Only after the TIP header is successfully loaded will proceed to step S5. Step S5: determining whether new liquid level detection parameters have been set in step S2; if so, performing liquid level detection according to the set new liquid level detection parameters and controlling the drive mechanism to reset; otherwise, controlling the drive mechanism to reset according to the default reset speed; The process proceeds to step S6 only after the liquid level is detected successfully, otherwise an alarm is issued and the process ends; The liquid level detection in step S5 includes air pressure liquid level detection and capacitance liquid level detection; Step S6: First, perform aspiration and then discharge, and then determine whether there is a suction blockage, a discharge blockage, or insufficient aspiration and discharge accuracy. If there is a suction blockage or a discharge blockage, an alarm will be issued and the process ends; If the suction and discharge accuracy is insufficient, the suction and discharge amount is adjusted by slightly increasing the pulse coefficient, and then the process proceeds to step S7; if there is no suction or discharge blockage or the suction and discharge accuracy is insufficient, the process proceeds directly to step S7; Step S7: Unload the TIP header and end the control.

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