Kit and automatic extraction equipment

Abstract

The invention discloses a kit and automatic extraction equipment, and relates to the technical field of molecular detection.The kit comprises a first body and a second body, the first body is provided with a reaction tube and a plurality of liquid storage tubes, the liquid storage tubes can convey liquid to the reaction tube under driving of an air pump, and magnetic beads are arranged in the reaction tube; the second body is rotationally connected to the first body, the end of the reaction tube is located in a containing cavity of the second body, and a first magnet and a second magnet are installed on the side wall of the containing cavity; when the second body rotates relative to the first body, the second body can be switched to a first position, a second position and a third position, when the second body is switched to the first position, the second position and the third position, the contact state of the reaction tube and the first magnet or the second magnet can be adjusted, and meanwhile the corresponding conduction state of the reaction tube is set. A magnet assembly does not need to be arranged outside the kit, so that the space utilization rate of the kit is increased, the operation convenience of the kit is improved, and the extraction efficiency of the kit is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of molecular detection, and in particular to a reagent kit and an automated extraction device. Background Art

[0002] Generally, the magnetic bead method is used in molecular detection such as nucleic acid extraction. The magnetic bead method can specifically identify and bind to nucleic acid molecules to obtain high-purity nucleic acid samples, and the binding efficiency of magnetic beads and nucleic acids is high, which improves the accuracy of the test results. At present, in order to improve the efficiency of nucleic acid detection, automated equipment combined with matching test kits are used for nucleic acid extraction, but the space utilization rate of the test kit combined with automated equipment is low, the operation of the test kit is cumbersome, and the extraction efficiency is low. Summary of the invention

[0003] The purpose of the present invention is to solve at least one of the technical problems existing in the prior art. To this end, the present invention provides a reagent kit to improve space utilization and extraction efficiency.

[0004] The invention also provides an automated extraction device having the reagent kit.

[0005] The reagent kit according to the embodiment of the first aspect of the present invention comprises: a first body and a second body, wherein the first body is provided with a reaction tube and a plurality of liquid storage tubes, the bottoms of the plurality of liquid storage tubes are connected to the reaction tubes through delivery tubes respectively; the plurality of liquid storage tubes are connected with air pumps respectively, the liquid storage tubes can deliver liquid to the reaction tubes under the drive of the air pumps, the bottoms of the reaction tubes extend out of the first body, and magnetic beads are arranged in the reaction tubes; the second body is rotatably connected to the first body, the second body is provided with a accommodating cavity, the end of the reaction tube is located in the accommodating cavity, the side walls of the accommodating cavity are provided with a first magnet and a second magnet, the first magnet and the second magnet The iron is arranged at intervals along the circumference of the second body, the first magnet and the second magnet can adsorb the magnetic beads, and the second body is provided with a waste liquid chamber and an extraction tube; the second body can be switched between a first position, a second position, and a third position when rotating relative to the first body, and when the second body is located at the first position, the side wall of the reaction tube contacts the first magnet, and the reaction tube is connected to the extraction tube; when the second body is located at the second position, the side wall of the reaction tube contacts the second magnet, and the reaction tube is connected to the waste liquid chamber; when the second body is in the third position, the reaction tube is disconnected from the extraction tube and the waste liquid chamber.

[0006] The reagent kit of the present invention has at least the following beneficial effects: the present embodiment is provided with a first body and a second body, and magnetic beads are arranged in the reaction tube, and the magnetic beads are used to absorb nucleic acid. Since a plurality of liquid storage tubes are connected to the reaction tube through a delivery tube, and the liquid storage tube can deliver liquid to the reaction tube under the drive of an air pump, and at the same time, the second body is in the third position, and the reaction tube is away from the first magnet and the second magnet, so that the reagent liquid enters the reaction tube and fully contacts and reacts with the magnetic beads. When the first magnet and the second magnet are in contact with the side wall of the reaction tube respectively, the magnetic beads can be adsorbed on the side wall of the reaction tube, which facilitates the liquid in the reaction tube to enter the extraction tube and the waste liquid chamber. By rotating the second body to adjust the conversion of the second body between the first position, the second position and the third position, the extraction of nucleic acid is gradually realized, which improves the convenience of operation of the reagent kit and improves the extraction efficiency. Since the first magnet and the second magnet are directly mounted on the second body, the end of the reaction tube is located in the accommodating cavity. When the second body switches between the first position, the second position and the third position, the contact state between the reaction tube and the first magnet or the second magnet can be adjusted, and the corresponding conduction state of the reaction tube can be adjusted at the same time. There is no need to set a magnet assembly on the outside of the test kit, which improves the space utilization of the test kit, improves the operation convenience of the test kit, and improves the extraction efficiency of the test kit.

[0007] According to some embodiments of the present invention, the first body is provided with an abutting portion, the end face of the abutting portion abuts against the end face of the second body, the abutting portion is provided with a connecting hole along its axial direction, the reaction tube is connected to the connecting hole through the delivery tube, when the second body is in the first position, the connecting hole is connected to the extraction tube, when the second body is in the second position, the connecting hole is connected to the waste liquid chamber, and when the second body is in the third position, the second body is blocked in the connecting hole.

[0008] According to some embodiments of the present invention, the bottom of the reaction tube extends out of the abutment portion, the bottom end of the reaction tube is connected to the connecting hole through the delivery tube, and along the axial direction of the first body, the connecting hole is higher than the bottom of the reaction tube.

[0009] According to some embodiments of the present invention, the liquid storage tube includes a sample tube, the sample tube is filled with sample liquid, and the air pump connected to the sample tube can transport the liquid in the reaction tube to the connecting hole.

[0010] According to some embodiments of the present invention, the second body is provided with an extraction hole along its axial direction, the extraction hole extends from the end surface of the second body to the extraction tube, the extraction tube is installed on the second body, and the connecting hole is connected to the extraction tube through the extraction hole.

[0011] According to some embodiments of the present invention, a first air permeable groove is provided on the end surface of the second body close to the first body, the first air permeable groove is connected to the extraction hole, the abutment portion is provided with an air permeable hole along the axial direction of the first body, and when the second body is in the first position, the air permeable hole is connected to the first air permeable groove.

[0012] According to some embodiments of the present invention, a second air permeable groove is provided on the end surface of the second body close to the first body, and the second air permeable groove is connected to the waste liquid chamber. When the second body is in the second position, the air hole is connected to the second air permeable groove.

[0013] According to some embodiments of the present invention, a first sealing groove and a plurality of second sealing grooves are provided at one end of the abutment portion close to the second body, the plurality of second sealing grooves are arranged at intervals along the circumference of the first body, the first sealing groove is connected to the connecting hole, a sealing ring is provided in the first sealing groove, and a sealing gasket is provided in the second sealing groove.

[0014] According to some embodiments of the present invention, the first magnet and the second magnet are respectively embedded in the side walls of the accommodating cavity.

[0015] According to a second aspect of the present invention, there is provided an automated extraction device, comprising a driving device and the embodiment disclosed in the first aspect of the present invention.

[0016] The automated extraction device according to the second embodiment of the present invention has at least the following beneficial effects: the automated extraction device adopts the reagent kit of the first embodiment of the present invention. The reagent kit is provided with a first body and a second body, and magnetic beads are provided in the reaction tube, and the magnetic beads are used to adsorb nucleic acids. Since a plurality of liquid storage tubes are connected to the reaction tube through a delivery tube, and the liquid storage tube can deliver liquid to the reaction tube under the drive of an air pump, and at the same time, the second body is in a third position, and the reaction tube is away from the first magnet and the second magnet, so that the reagent liquid enters the reaction tube and fully contacts and reacts with the magnetic beads. When the first magnet and the second magnet are in contact with the side wall of the reaction tube respectively, the magnetic beads can be adsorbed on the side wall of the reaction tube, which facilitates the liquid in the reaction tube to enter the extraction tube and the waste liquid chamber. By rotating the second body to adjust the conversion of the second body between the first position, the second position and the third position, the extraction of nucleic acids is gradually realized, which improves the convenience of operation of the reagent kit and improves the extraction efficiency. Since the first magnet and the second magnet are directly mounted on the second body, the end of the reaction tube is located in the accommodating cavity. When the second body switches between the first position, the second position and the third position, the contact state between the reaction tube and the first magnet or the second magnet can be adjusted, and the corresponding conduction state of the reaction tube can be adjusted at the same time. There is no need to set a magnet assembly on the outside of the test kit, which improves the space utilization of the test kit, improves the operation convenience of the test kit, and improves the extraction efficiency of the test kit.

[0017] Additional aspects and advantages of the present invention will be given in part in the following description and in part will be obvious from the following description, or will be learned through practice of the present invention. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The present invention is further described below in conjunction with the accompanying drawings and embodiments; Figure 1 This is a schematic diagram of the structure of the kit in an embodiment of the present invention; Figure 2 for Figure 1 Cross-sectional view of the middle AA part; Figure 3 An explosion diagram of a reagent kit in an embodiment of the present invention; Figure 4 An exploded schematic diagram of a first body in an embodiment of the present invention; Figure 5 is a schematic structural diagram of a first body in an embodiment of the present invention; Figure 6 This is a schematic diagram of the bottom structure of the first body in an embodiment of the present invention; Figure 7 An exploded schematic diagram of a mounting base and a housing in an embodiment of the present invention; Figure 8 This is a schematic diagram of the top structure of the second body in an embodiment of the present invention; Fig. 9 for Figure 8 Cross-sectional view of the middle BB area; Fig.10 for Figure 8 Cross-sectional view of the CC area.

[0019] Reference numerals: Kit 10; First body 100; first mounting hole 101; first mounting groove 102; second mounting hole 103; second mounting groove 104; second protrusion 105; first hole 106; abutment portion 110; connecting hole 111; vent hole 112; reaction tube 120; first mounting ring 121; mounting block 130; conducting hole 131; vent hole 132; sealing tube 133; Liquid storage tube 200; second mounting ring 201; sample tube 210; lysis tube 220; cleaning tube 230; de-washing tube 240; amplification tube 250; Second body 300; accommodating chamber 301; waste liquid chamber 302; extraction hole 303; first air permeable groove 304; second air permeable groove 305; first sealing groove 306; second sealing groove 307; third mounting groove 308; second hole 309; first magnet 310; first magnet chamber 311; second magnet 320; second magnet chamber 321; extraction tube 330; third mounting ring 331; connecting member 340; transmission member 350; Delivery pipe 400; Air pump 500; Sealing plate 600; first air inlet hole 610; third sealing groove 620; third protrusion 630; Mounting seat 700; second air inlet hole 710; receiving groove 720; first fixing groove 721; Housing 800; first protruding portion 810; second fixing groove 820; sampling port 830; transmission port 840. DETAILED DESCRIPTION

[0020] This section will describe in detail the specific embodiments of the present invention. The preferred embodiments of the present invention are shown in the accompanying drawings. The purpose of the accompanying drawings is to supplement the description of the text part of the specification with graphics, so that people can intuitively and vividly understand each technical feature and the overall technical solution of the present invention, but it cannot be understood as a limitation on the scope of protection of the present invention.

[0021] In the description of the present invention, it should be understood that descriptions involving orientations, such as up, down, front, back, left, right, etc., and orientations or positional relationships indicated are based on the orientations or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be understood as a limitation on the present invention.

[0022] In the description of the present invention, "several" means one or more, "more" means more than two, "greater than", "less than", "exceed" etc. are understood as not including the number itself, and "above", "below", "within" etc. are understood as including the number itself. If there is a description of "first" or "second", it is only used for the purpose of distinguishing the technical features, and cannot be understood as indicating or implying the relative importance or implicitly indicating the number of the indicated technical features or implicitly indicating the order of the indicated technical features.

[0023] In the description of the present invention, unless otherwise clearly defined, terms such as setting, installing, connecting, etc. should be understood in a broad sense, and technicians in the relevant technical field can reasonably determine the specific meanings of the above terms in the present invention based on the specific content of the technical solution.

[0024] Reference Figure 2 and Figure 3The first embodiment of the present invention proposes a reagent kit 10, including a first body 100 and a second body 300, wherein the first body 100 is rotatably connected to the second body 300. The first body 100 is equipped with a reaction tube 120 and a plurality of liquid storage tubes 200. Along the axial direction of the liquid storage tube 200, the bottoms of the plurality of liquid storage tubes 200 are connected to the reaction tube 120 through the delivery tube 400, respectively, and the plurality of liquid storage tubes 200 are respectively connected to the air pump 500, and the liquid storage tube 200 can deliver liquid to the reaction tube 120 under the drive of the air pump 500, and the reaction tube 120 is provided with magnetic beads. The magnetic beads are used to adsorb nucleic acids, and under the drive of the air pump 500, the reagent liquids in the plurality of liquid storage tubes 200 respectively enter the reaction tube 120 for reaction, so that the purity of the nucleic acid adsorbed by the magnetic beads is getting higher and higher, thereby realizing the extraction of nucleic acids.

[0025] It can be understood that the first body 100, the second body 300, the liquid storage tube 200, and the reaction tube 120 are respectively provided with a central axis, the direction along the central axis is the axial direction, the direction around the central axis is the circumferential direction, and the radial direction is perpendicular to the axial direction. In the radial direction, the direction away from the central axis is radially outward, and the direction close to the central axis is radially inward. In the axial direction of the reaction tube 120, the bottom of the reaction tube 120 is the end of the reaction tube 120 close to the second body 300. In the axial direction of the liquid storage tube 200, the bottom of the liquid storage tube 200 is the end of the liquid storage tube 200 close to the second body 300.

[0026] Reference Figure 2 and Figure 3 In some embodiments of the present invention, the second body 300 is provided with a receiving chamber 301, and the opening of the receiving chamber 301 faces the first body 100. The bottom of the reaction tube 120 extends out of the first body 100, and the end of the reaction tube 120 is located in the receiving chamber 301. By rotating the second body 300, the reaction tube 120 can move along the circumference of the second body 300. The side wall of the receiving chamber 301 is installed with a first magnet 310 and a second magnet 320, and the first magnet 310 and the second magnet 320 are arranged at intervals along the circumference of the second body 300. The first magnet 310 and the second magnet 320 can absorb magnetic beads. The second body 300 is provided with a waste liquid chamber 302 and an extraction tube 330. The waste liquid chamber 302 is used to collect waste liquid generated during the extraction process, and the extraction chamber is used to collect liquid after the extraction is completed.

[0027] Reference Figure 2 and Figure 3, the second body 300 can be switched to the first position, the second position and the third position when rotating relative to the first body 100. When the second body 300 is in the first position, the side wall of the reaction tube 120 contacts the first magnet 310, and the reaction tube 120 is connected with the extraction tube 330. At this time, the liquid in the reaction tube 120 can enter the extraction tube 330, and the magnetic beads are located on the side wall of the reaction tube 120, which reduces the risk of the magnetic beads flowing out of the reaction tube 120, and the magnetic beads do not hinder the liquid in the reaction tube 120 from flowing into the extraction tube 330. When the second body 300 is in the second position, the side wall of the reaction tube 120 contacts the second magnet 320, and the reaction tube 120 is connected with the waste liquid chamber 302. At this time, the liquid in the reaction tube 120 can enter the waste liquid chamber 302, and the magnetic beads are located on the side wall of the reaction tube 120, which reduces the risk of the magnetic beads flowing out of the reaction tube 120, and the magnetic beads do not hinder the liquid in the reaction tube 120 from flowing into the waste liquid chamber 302. When the second body 300 is located at the third position, the reaction tube 120 is away from the first magnet 310 and the second magnet 320, and is disconnected from the extraction tube 330 and the waste liquid chamber 302. At this time, the reagent in the liquid storage tube 200 is driven into the reaction tube 120 by the air pump 500, and the magnetic beads are located in the reaction tube 120 and flow freely, so that the reagent can fully contact and react with the magnetic beads, ensuring the accuracy of extraction. Since the reaction tube 120 is disconnected from the extraction tube 330 and the waste liquid chamber 302, so that the reagent enters the reaction tube 120 and is stored in the reaction tube 120, the reagent can fully contact and react with the magnetic beads, ensuring the accuracy of extraction.

[0028] It is understandable that the air pump 500 controls the liquid in the liquid storage tube 200 to enter the reaction tube 120, and the second body 300 is rotated, so that the second body 300 is switched between the first position, the second position and the third position, and the nucleic acid extraction is gradually realized, which improves the convenience of operation of the reagent kit 10 and improves the extraction efficiency. Since the first magnet 310 and the second magnet 320 are directly mounted on the second body 300, the end of the reaction tube 120 is located in the accommodating cavity 301 of the second body 300, so that the contact state between the reaction tube 120 and the first magnet 310 or the second magnet 320 can be adjusted by rotating the second body 300, and there is no need to set a magnet assembly outside the reagent kit 10, which improves the space utilization of the reagent kit 10, improves the convenience of operation of the reagent kit 10, and thus improves the extraction efficiency of the reagent kit 10.

[0029] Reference Figure 5 and Figure 6In some embodiments of the present invention, the first body 100 is provided with an abutting portion 110, the end surface of the abutting portion 110 abuts against the end surface of the second body 300, and the abutting portion 110 is provided with a connecting hole 111 along its axial direction. The reaction tube 120 is connected to the connecting hole 111 through the delivery tube 400, and the connecting hole 111 is connected to the extraction tube 330 through the delivery tube 400. When the second body 300 is in the first position, the connecting hole 111 is connected to the waste liquid chamber 302. When the second body 300 is in the third position, the end surface of the second body 300 is blocked by the connecting hole 111. The liquid in the delivery tube 400 is guided to flow into the extraction tube 330 or the extraction chamber through the connecting hole 111 of the first body 100, thereby improving the accuracy of liquid transportation in the reaction tube 120.

[0030] Reference Figure 5 and Figure 6 In some embodiments of the present invention, the bottom of the reaction tube 120 extends out of the abutment portion 110, and along the axial direction of the reaction tube 120, the bottom end of the reaction tube 120 is connected to the connecting hole 111 through the delivery tube 400, and along the axial direction of the first body 100, the connecting hole 111 is higher than the bottom of the reaction tube 120. Since the bottom end of the reaction tube 120 is connected to the connecting hole 111 through the delivery tube 400, the liquid in the reaction tube 120 can fully enter the connecting hole 111 from the bottom of the reaction tube 120, reducing the possibility of liquid residue in the reaction tube 120, thereby improving the extraction purity. By making the connecting hole 111 higher than the bottom of the reaction tube 120, the outlet end of the delivery tube 400 connected to the reaction tube 120 is higher than the bottom of the reaction tube 120. When the second body 300 is in the third position, the possibility of the liquid in the reaction tube 120 overflowing from the outlet end of the delivery tube 400 is reduced, thereby improving the sealing performance of the reagent kit 10 and improving the stability of the detection of the reagent kit 10. The bottom of the reaction tube 120 extends out of the abutment portion 110, and the waste liquid chamber 302 and the extraction tube 330 are connected to the end surface of the second body 300 contacting the abutment portion 110, so that the waste liquid chamber 302, the first magnet 310 and the second magnet 320 are arranged at intervals along the circumference of the second body 300, making the spatial arrangement of the reagent kit 10 more compact and improving the space utilization rate of the reagent kit 10.

[0031] Reference Figure 3 and Figure 4In some embodiments of the present invention, the liquid storage tube 200 includes a sample tube 210, the sample tube 210 is filled with sample liquid, and the air pump 500 connected to the sample tube 210 can transport the liquid in the reaction tube 120 to the connecting hole 111. In a single test, the sample liquid in the sample tube 210 is first completely input into the reaction tube 120, and in subsequent tests, the air pump 500 connected to the sample tube 210 transports the liquid in the reaction tube 120 to the connecting hole 111, so that the liquid in the reaction tube 120 enters the waste liquid chamber 302 or the extraction tube 330. The reaction tube 120 does not need to be separately connected to the air pump 500, which saves costs. At the same time, the structure of the reagent kit 10 is more compact and the space utilization rate is improved.

[0032] Reference Figure 8 In some embodiments of the present invention, the first magnet 310 and the second magnet 320 are respectively embedded in the side wall of the accommodating chamber 301, so that when the second body 300 is rotated, the end of the reaction tube 120 can have enough movement space in the accommodating chamber 301. The side wall of the accommodating chamber 301 is provided with a first magnet 310 cavity and a second magnet 320 cavity, and the first magnet 310 cavity and the second magnet 320 cavity are arranged at intervals along the circumference of the second body 300. The first magnet 310 is installed in the first magnet 310 cavity, and the second magnet 320 is installed in the second magnet 320 cavity. By setting the first magnet 310 cavity and the second magnet 320 cavity, the first magnet 310 and the second magnet 320 are respectively embedded in the side wall of the accommodating chamber 301, and at the same time, the installation space of the first magnet 310 and the second magnet 320 is guaranteed.

[0033] Reference Figure 8 and Fig. 9 In some embodiments of the present invention, the second body 300 is provided with an extraction hole 303 along its axial direction, the extraction hole 303 extends from the end surface of the second body 300 to the extraction tube 330, the extraction tube 330 is installed on the second body 300, and the connecting hole 111 is connected to the extraction tube 330 through the extraction hole 303. Since the extraction tube 330 is installed on the second body 300, after the extraction is completed, the extraction tube 330 can be directly removed from the second body 300, so as to facilitate the extraction of the extract from the reagent kit 10.

[0034] Reference Figure 3 and Fig. 9In some embodiments of the present invention, the extraction tube 330 is provided with a third mounting ring 331, and the third mounting ring 331 extends outward from the outer wall of the extraction tube 330 along the radial direction of the third mounting ring 331. The second body 300 is provided with a third mounting groove 308, and the third mounting groove 308 extends inward from the outer wall of the second body 300 along the radial direction of the second body 300. The extraction tube 330 is installed in the third mounting groove 308, the third mounting ring 331 abuts against the side wall of the third mounting groove 308, the extraction hole 303 is connected to the top wall of the third mounting groove 308, and the end face of the opening of the extraction tube 330 abuts against the top wall of the third mounting groove 308. Since the end face of the opening of the extraction tube 330 abuts against the top wall of the third mounting groove 308, the sealing performance of the extraction tube 330 is improved, the possibility of the extraction liquid in the extraction tube 330 being contaminated is reduced, and the possibility of the extraction liquid overflowing from the opening of the extraction tube 330 is reduced, thereby improving the accuracy of the detection result. Since the opening of the third mounting groove 308 is connected to the outer wall of the second body 300, after the first body 100 and the second body 300 are connected, there is no need to disassemble the first body 100 and the second body 300, and the extraction tube 330 can be directly installed and removed from the opening of the third mounting groove 308, thereby reducing the difficulty of installation and maintenance. At the same time, after the reagent kit 10 completes a test, the extraction liquid in the extraction tube 330 can be transferred in time, so that the reagent kit 10 can continuously extract multiple sample liquids, thereby improving the detection efficiency.

[0035] Reference Figure 6 , Figure 8 and Fig. 9 In some embodiments of the present invention, the end surface of the second body 300 close to the first body 100 is provided with a first air-permeable groove 304, the first air-permeable groove 304 is connected to the extraction hole 303, and the abutting portion 110 is provided with an air-permeable hole 112 along the axial direction of the first body 100. When the second body 300 is in the first position, the air-permeable hole 112 is connected to the first air-permeable groove 304. Since the air-permeable hole 112 is connected to the first air-permeable groove 304, the extraction tube 330 is connected to the air-permeable hole 112, so that the air pressure inside and outside the extraction tube 330 is balanced, and then the liquid in the reaction tube 120 can flow into the extraction tube 330, thereby ensuring the reliability of the reagent kit 10.

[0036] Reference Figure 6 , Figure 8 and Fig.10In some embodiments of the present invention, the second body 300 is provided with a second vent groove 305 on the end surface close to the first body 100, and the second vent groove 305 is connected to the waste liquid chamber 302. When the second body 300 is in the second position, the vent hole 112 is connected to the second vent groove 305. Since the vent hole 112 is connected to the second vent groove 305, the waste liquid chamber 302 is connected to the vent hole 112, so that the air pressure inside and outside the waste liquid chamber 302 is balanced, so that the liquid in the reaction tube 120 can smoothly flow into the waste liquid chamber 302, thereby ensuring the reliability of the reagent kit 10.

[0037] Reference Figure 6 In some embodiments of the present invention, a first sealing groove and a plurality of second sealing grooves 307 are provided at one end of the abutting portion 110 close to the second body 300. The plurality of second sealing grooves 307 are arranged at intervals along the circumference of the first body 100. The first sealing groove 306 is in communication with the connecting hole 111. A sealing ring is provided in the first sealing groove 306, and a sealing gasket is provided in the second sealing groove 307. Since a sealing ring is provided in the first sealing groove 306, and the sealing ring has a through hole, the liquid in the connecting hole 111 can flow into the waste liquid chamber 302 or the extraction tube 330 from the through hole. Since the first sealing groove 306 is provided with a sealing ring, the sealing performance of the connecting hole 111 is improved, the possibility of liquid overflowing from the connecting hole 111 is reduced, and the possibility of liquid in the extraction tube 330 being contaminated is reduced, thereby improving the sealing performance and reliability of the reagent kit 10. Since the abutting portion 110 is provided with a plurality of second sealing grooves 307, and a sealing gasket is provided in the sealing groove, the sealing performance between the first body 100 and the second body 300 is improved, the possibility of the extract being contaminated is reduced, and the reliability of the detection of the reagent kit 10 is improved. At the same time, the rotation stability of the second body 300 relative to the first body 100 is improved, the possibility of wear between the first body 100 and the second body 300 is reduced, and the service life of the first body 100 and the second body 300 is increased. Since the plurality of second sealing grooves 307 are arranged at intervals along the circumference of the first body 100, the force on the contact surface of the second body 300 and the abutment portion 110 is uniform, and the rotation stability of the second body 300 relative to the first body 100 is improved.

[0038] It is understandable that in some embodiments of the present invention, the sealing ring can be fixed to the second sealing groove 307 by glue, and the sealing pad can be fixedly connected to the second sealing groove 307 by glue, so that the sealing ring is fixed to the second sealing groove 307, and the sealing pad is fixed to the second sealing groove 307. When the second body 300 is rotated, the possibility of the sealing pad and the sealing ring affecting the first air permeable groove 304 and the second air permeable groove 305 is reduced, thereby ensuring the stability of the rotation of the first body 100 relative to the second body 300. The sealing ring and the first sealing groove 306 can also be fixed in such a way that an embedding groove is provided in the first sealing groove 306 and a corresponding part of the sealing ring is embedded in the embedding groove. The fixing method of the sealing ring and the first sealing groove 306, and the fixing method of the sealing pad and the second sealing groove 307 can be selected according to actual applications, and the present application does not limit it here.

[0039] Reference Figure 3 and Figure 4 In some embodiments of the present invention, the first body 100 is provided with a first mounting hole 101 and a first mounting groove 102 that are interconnected along its axial direction, the first mounting groove 102 extends from the end surface of the first body 100 away from the second body 300 to the direction close to the second body 300, and the reaction tube 120 is plugged into the first mounting hole 101. The reaction tube 120 is provided with a first mounting ring 121, and the first mounting ring 121 abuts against the bottom wall of the first mounting groove 102, so that the reaction tube 120 is fixedly installed on the first body 100. Since the reaction tube 120 can be directly inserted into the first mounting hole 101 from the end surface of the first body 100, and the first mounting ring 121 abuts against the bottom wall of the first mounting groove 102, it is convenient for the reaction tube 120 to be directly plugged into the first body 100, thereby improving the convenience of installation and removal of the reaction tube 120. A mounting block 130 is provided in the first mounting groove 102. The mounting block 130 presses against the end surface of the opening of the reaction tube 120 to seal the reaction tube 120, thereby reducing the possibility of contamination of the liquid in the reaction tube 120. At the same time, the sealing of the reaction tube 120 is ensured, so that the sample liquid connection air pump 500 can transport the liquid in the reaction tube 120 to the connecting hole 111, thereby ensuring the reliability of the reagent kit 10.

[0040] Reference Figure 2 and Figure 4 In some embodiments of the present invention, the mounting block 130 is provided with a sealing tube 133, the sealing tube 133 is inserted into the reaction tube 120, and the outer wall of the sealing tube 133 abuts against the inner wall of the reaction tube 120. Since the mounting block 130 abuts against the end surface of the opening of the reaction tube 120, and the outer wall of the sealing tube 133 abuts against the inner wall of the reaction tube 120, the sealing performance of the reaction tube 120 is improved, and the possibility of contamination of the liquid in the reaction tube 120 is reduced. At the same time, it is ensured that the sample liquid connection air pump 500 can transport the liquid in the reaction tube 120 to the connecting hole 111.

[0041] Reference Figure 4 In some embodiments of the present invention, the mounting block 130 is provided with a plurality of conducting holes 131 along its axial direction, and the conducting holes 131 are connected to the reaction tube 120. The bottom of the liquid storage tube 200 is connected to the conducting hole 131 through the delivery tube 400. Under the drive of the air pump 500, the liquid in the liquid storage tube 200 is input into the reaction tube 120 through the delivery tube 400 from the bottom of the liquid storage tube 200. The plurality of conducting holes 131 correspond to the delivery tubes 400 of the plurality of liquid storage tubes 200. Since the liquid in the liquid storage tube 200 enters the reaction tube 120 through the conducting holes 131, that is, the liquid flows into the reaction tube 120 from the open end of the reaction tube 120, the possibility of the liquid impacting the magnetic beads at the bottom of the reaction tube 120 is increased, thereby making the liquid and the magnetic beads fully contact and react. The outlet end of the delivery tube 400 connected to the liquid storage tube 200 is higher than the bottom of the liquid storage tube 200, which reduces the possibility of the liquid in the liquid storage tube 200 overflowing from the outlet end of the delivery tube 400, improves the stability of the reagent transported by the liquid storage tube 200, reduces the risk of affecting the test results, and thereby improves the stability of the test kit 10.

[0042] It is understandable that, driven by the air pump 500, the reagent in the liquid storage tube 200 enters the reaction tube 120 from the delivery tube 400, and by controlling the delivery pressure of the air pump 500, the flow rate of the reagent from the liquid storage tube 200 into the reaction tube 120 is accurately controlled, and the amount of the reagent entering the reaction tube 120 is controlled, thereby improving the accuracy and reliability of the detection. At the same time, the delivery of liquid by the air pump 500 reduces the possibility of liquid remaining in the tube, reduces the loss of liquid during the detection process, and saves detection costs.

[0043] Reference Figure 4 In some embodiments of the present invention, the mounting block 130 is provided with a vent hole 132 along its axial direction, and the vent hole 132 connects the reaction tube 120 and the first mounting groove 102. When the air pump 500 connected to the sample liquid drives the liquid in the reaction tube 120 to flow into the connecting hole 111, the vent hole 132 can reduce the probability of liquid backflow in the delivery tube 400 connected to the liquid storage tube 200, thereby reducing the possibility of excessive pressure in the liquid storage tube 200, and improving the accuracy of controlling the amount of reagent liquid flowing into the reaction tube 120.

[0044] Reference Figure 4 and Figure 5In some embodiments of the present invention, the second body 300 is provided with a second mounting hole 103 and a second mounting groove 104 that are interconnected, the second mounting groove 104 extending from the end surface of the first body 100 away from the second body 300 to the direction close to the second body 300, and the liquid storage tube 200 is plugged into the second mounting hole 103. The liquid storage tube 200 is provided with a second mounting ring 201, and the second mounting ring 201 abuts against the bottom wall of the second mounting groove 104, so that the liquid storage tube 200 is fixedly installed on the second body 300. Since the liquid storage tube 200 can be directly inserted into the second mounting hole 103 from the end surface of the first body 100, and the second mounting ring 201 abuts against the bottom wall of the second mounting groove 104, it is convenient for the reaction tube 120 to be directly plugged into the first body 100, thereby improving the convenience of installation and removal of the reaction tube 120. It can be understood that the second body 300 is provided with a plurality of second mounting holes 103 and a plurality of second mounting grooves 104 , and the plurality of liquid storage tubes 200 correspond to the plurality of second mounting grooves 104 one by one.

[0045] Reference Figure 2 and Figure 4 In some embodiments of the present invention, the reagent box 10 is further provided with a sealing plate 600 and a mounting seat 700, and the two surfaces of the sealing plate 600 are respectively in contact with the end surface of the first body 100 and the mounting seat 700. The sealing plate 600 is pressed against the end surfaces of the openings of several liquid storage tubes 200 to keep the liquid storage tubes 200 sealed, so that the air pump 500 can drive the liquid in the liquid storage tubes 200 into the reaction tube 120. Along the axial direction of the liquid storage tube 200, since the second mounting ring 201 of the liquid storage tube 200 is in contact with the bottom wall of the second mounting groove 104, and the end surface of the opening of the liquid storage tube 200 is in contact with the sealing plate 600, so that the liquid storage tube 200 is fixed in the first body 100, the risk of shaking of the liquid storage tube 200 during detection of the reagent box 10 is reduced. The sealing plate 600 is provided with a plurality of first air inlet holes 610, and the mounting base 700 is provided with a plurality of second air inlet holes 710. The plurality of first air inlet holes 610 and the plurality of second air inlet holes 710 are connected one by one, and the plurality of first air inlet holes 610 are connected one by one with the plurality of liquid storage tubes 200. The plurality of air pumps 500 are installed on a side of the mounting base 700 away from the sealing plate 600, and the plurality of air pumps 500 are connected one by one with the plurality of second air inlet holes 710, so that the plurality of air pumps 500 can respectively control the flow of reagents in the plurality of liquid storage tubes 200 into the reaction tube 120.

[0046] Refer to 4 and Figure 5In some embodiments of the present invention, a plurality of third sealing grooves 620 are provided on the surface of the sealing plate 600 facing away from the first body 100, and the plurality of third sealing grooves 620 are respectively connected to the plurality of first air inlet holes 610 in a one-to-one correspondence. A sealing ring is provided inside the third sealing gasket, and the through hole inside the sealing ring enables the first air inlet hole 610 to be connected to the second air inlet hole 710. By providing a sealing ring between the first air inlet hole 610 and the second air inlet hole 710, the sealing between the first air inlet hole 610 and the second air inlet hole 710 is improved, and the accuracy of the air pump 500 in controlling the flow of liquid in the liquid storage tube 200 is improved. At the same time, the risk of the liquid in the liquid storage tube 200 being blocked is reduced.

[0047] Reference Figure 2 , Figure 5 and Figure 7 In some embodiments of the present invention, the end surface of the mounting seat 700 close to the sealing seat is provided with a receiving groove 720, and the sealing plate 600 is located in the receiving groove 720. To prevent the sealing plate 600 from escaping from the mounting seat 700. The reagent box 10 is also provided with a shell 800, which is sleeved on the first body 100 and the second body 300, and the shell 800 is provided with an opening, and one end of the shell 800 with the opening abuts against the bottom wall of the receiving groove 720, and the shell 800 is fixedly connected to the mounting seat 700. The outer wall of the shell 800 is provided with a first protrusion 810, and the first protrusion 810 is located at one end of the shell 800 close to the mounting seat 700, and the side wall of the receiving groove 720 is provided with a first fixing groove 721, and the first protrusion 810 is located in the first fixing groove 721, so that the positioning of the shell 800 relative to the mounting seat 700 prevents the shell 800 and the mounting seat 700 from rotating relative to each other, thereby improving the stability of the reagent box 10. The inner wall of the housing 800 is provided with a second fixing groove 820, and the outer wall of the first body 100 is provided with a second protrusion 105, which is located at one end of the first body 100 close to the sealing plate 600, and the second protrusion 105 is located in the second fixing groove 820, so that the first body 100 is positioned relative to the housing 800, reducing the risk of relative rotation between the first body 100 and the housing 800, thereby ensuring the connection between the liquid storage tube 200 in the first body 100 and the air pump 500, and improving the reliability of the reagent kit 10. The sealing plate 600 is provided with a third protrusion 630, and the third protrusion 630 is located in the second fixing groove 820, so that the sealing plate 600 is positioned relative to the housing 800, and then the sealing plate 600 is positioned relative to the first body 100, reducing the risk of rotation of the sealing plate 600 relative to the first body 100, thereby ensuring the first air inlet 610, the second air inlet 710 and the liquid storage tube 200 are connected, and improving the reliability of the reagent kit 10.

[0048] Reference Figure 1 and Figure 3In some embodiments of the present invention, a sampling port 830 is provided on the side wall of the housing 800, and the sampling port 830 can expose the extraction tube 330. The extraction tube 330 can be installed and removed through the sampling port 830, thereby improving the convenience of installing and removing the extraction tube 330.

[0049] Reference Figure 2 and Figure 8 , the first body 100 and the second body 300 are connected by a connecting piece 340, a first hole 106 is provided at one end of the first body 100 close to the second body 300, and a second hole 309 is provided in the axial direction of the second body 300, and the second hole 309 extends from the end surface of the second body 300 away from the first body 100 to the accommodating cavity 301. The connecting piece 340 passes through the first hole 106 and the second hole 309, and the end of the connecting piece 340 is fixedly connected to the first hole 106, so that the second body 300 can rotate relative to the connecting piece 340, thereby realizing the rotation of the second body 300 relative to the second body 300. It can be understood that the fixing method of the connecting piece 340 and the first body 100 can be threaded connection or interference fit, which can be selected according to actual production reference, and the present application is not limited here.

[0050] Reference Figure 3 and Figure 4 In some embodiments of the present invention, five liquid storage tubes 200 are provided, and the five liquid storage tubes 200 are arranged at intervals along the circumference of the first body 100. The five liquid storage tubes 200 respectively include a sample tube 210, a lysis tube 220, a cleaning tube 230, a de-elution tube 240 and an amplification tube 250. The sample tube 210 is filled with a sample liquid. The lysis tube 220 is filled with a lysis solution, and the lysis solution is used for the lysis operation of the sample liquid. The cleaning tube 230 is filled with a cleaning solution, and the cleaning solution is used for the cleaning operation. The de-elution tube 240 is filled with a de-elution solution, and the de-elution solution is used for the de-elution operation. The amplification tube 250 is filled with an amplification solution, and the amplification solution is used for the amplification operation. The reagents are sequentially input into the reaction tube 120 through the five liquid storage tubes 200 for lysis, cleaning, de-elution and amplification operations to obtain a purified solution.

[0051] Reference Figures 1 to 10 The steps of extracting nucleic acid in the kit 10 of some embodiments of the present invention are as follows: S1: Load the sample liquid into the sample tube 210, the lysis tube 220 into the lysis solution, the cleaning tube 230 into the cleaning solution, the de-elution tube 240 into the de-elution solution, the amplification tube 250 into the amplification solution, and the reaction tube 120 into the magnetic beads, and install the reagent kit 10 as a whole.

[0052] S2: The second body 300 is rotated to the third position, the air pump 500 connected to the sample tube 210 drives the sample liquid to completely enter the reaction tube 120, and the air pump 500 connected to the lysis tube 220 drives the lysis liquid to enter the reaction tube 120, and the lysis liquid, sample liquid and magnetic beads contact in the reaction tube 120 to perform a lysis operation.

[0053] S3: After the lysis operation is completed, the second body 300 is rotated to the second position. At this time, the second magnet 320 adsorbs the magnetic beads to the side wall of the sample tube 210, the reaction tube 120 is connected to the waste liquid chamber 302, and the air pump 500 connected to the sample tube 210 drives the liquid in the reaction tube 120 to enter the waste liquid chamber 302.

[0054] S4: The second body 300 is rotated to the third position, and the air pump 500 connected to the cleaning tube 230 drives the cleaning liquid into the reaction tube 120, and the cleaning liquid contacts the magnetic beads in the reaction tube 120 and performs a cleaning operation.

[0055] S5: After the cleaning operation is completed, the second body 300 is rotated to the second position. At this time, the second magnet 320 adsorbs the magnetic beads to the side wall of the sample tube 210, the reaction tube 120 is connected to the waste liquid chamber 302, and the air pump 500 connected to the sample tube 210 drives the liquid in the reaction tube 120 to enter the waste liquid chamber 302.

[0056] S6: The second body 300 is rotated to the third position, and the air pump 500 connected to the de-eluting liquid drives the de-eluting liquid into the reaction tube 120, and the de-eluting liquid contacts the magnetic beads in the reaction tube 120 and performs a de-eluting operation.

[0057] S7: After the elution operation is completed, the second body 300 is rotated to the second position. At this time, the second magnet 320 adsorbs the magnetic beads to the side wall of the sample tube 210, the reaction tube 120 is connected to the waste liquid chamber 302, and the air pump 500 connected to the sample tube 210 drives the liquid in the reaction tube 120 to enter the waste liquid chamber 302.

[0058] S8: The second body 300 is rotated to the third position, and the air pump 500 connected to the amplification liquid drives the amplification liquid into the reaction tube 120. The amplification liquid contacts the magnetic beads in the reaction tube 120 and performs an amplification operation to obtain an extraction liquid.

[0059] S9: Rotate the second body 300 to the first position. At this time, the first magnet 310 adsorbs the magnetic beads to the side wall of the sample tube 210, the reaction tube 120 is connected to the extraction tube 330, and the air pump 500 connected to the sample tube 210 drives the extraction liquid in the reaction tube 120 to enter the extraction tube 330.

[0060] It is understood that the cleaning operation can be performed twice or more to improve the cleaning effect. If multiple cleaning operations are performed, steps S4 and S5 are repeated after completing step S5. The number of times the cleaning operation is performed can be selected according to the implementation of the production application, and this application is not limited here.

[0061] The second embodiment of the present invention provides an automated extraction device, comprising a driving device and the reagent kit 10 of the first embodiment. The driving device can drive the second body 300 to rotate, thereby realizing the rotation of the second body 300 relative to the first body 100, reducing manual input and improving extraction efficiency.

[0062] Reference Figure 2 and Figure 3 In some embodiments of the present invention, the end of the second body 300 away from the first body 100 is connected to the transmission member 350, and the housing 800 is provided with a transmission opening 840, which is located at the end of the housing 800 away from the mounting seat 700, and the transmission opening 840 exposes the transmission member 350. The transmission member 350 is connected to a driving device, and the driving device can drive the transmission member 350 to rotate, thereby driving the second body 300 to rotate. Since the transmission opening 840 exposes the transmission member 350, it is convenient for the driving device to be directly connected to the transmission member 350.

[0063] It is understandable that the driving device can be a motor or a combination of a cylinder and a mechanical component to drive the second body 300 to rotate. It can be selected according to actual reference and this application does not limit it here.

[0064] Since the automated extraction device adopts all the technical solutions of the reagent kit 10 of the above embodiment, it has at least all the beneficial effects brought by the technical solutions of the above embodiment, which will not be described in detail here.

[0065] In the description of this specification, the description with reference to the terms "one embodiment", "some embodiments", "illustrative embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described may be combined in any one or more embodiments or examples in a suitable manner.

[0066] Although the embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the claims and their equivalents.

Claims

1. A kit, characterized in that: include: The first body is provided with a reaction tube and a plurality of liquid storage tubes, the bottoms of the plurality of liquid storage tubes are connected to the reaction tube through delivery tubes respectively; the plurality of liquid storage tubes are connected to air pumps respectively, the liquid storage tubes can deliver liquid to the reaction tube under the drive of the air pumps, the bottom of the reaction tube extends out of the first body, and magnetic beads are arranged in the reaction tube; A second body is rotatably connected to the first body, the second body is provided with a receiving cavity, the end of the reaction tube is located in the receiving cavity, a first magnet and a second magnet are installed on the side wall of the receiving cavity, the first magnet and the second magnet are arranged at intervals along the circumference of the second body, the first magnet and the second magnet can absorb the magnetic beads, and the second body is provided with a waste liquid cavity and an extraction tube; The second body can be switched to a first position, a second position, and a third position when rotating relative to the first body. When the second body is located at the first position, the side wall of the reaction tube contacts the first magnet, and the reaction tube is connected to the extraction tube; when the second body is located at the second position, the side wall of the reaction tube contacts the second magnet, and the reaction tube is connected to the waste liquid chamber; When the second body is in the third position, the reaction tube is disconnected from the extraction tube and the waste liquid chamber.

2. The kit according to claim 1, characterized in that: The first body is provided with an abutting portion, the end face of the abutting portion abuts against the end face of the second body, the abutting portion is provided with a connecting hole along its axial direction, the reaction tube is connected to the connecting hole through the delivery tube, when the second body is in the first position, the connecting hole is connected to the extraction tube, when the second body is in the second position, the connecting hole is connected to the waste liquid chamber, and when the second body is in the third position, the second body is blocked in the connecting hole.

3. The kit according to claim 2, characterized in that: The bottom of the reaction tube extends out of the abutment portion, and the bottom end of the reaction tube is connected to the connecting hole through the delivery tube. Along the axial direction of the first body, the connecting hole is higher than the bottom of the reaction tube.

4. The kit according to claim 2, characterized in that: The liquid storage tube comprises a sample tube, the sample tube is filled with sample liquid, and the air pump connected to the sample tube can transport the liquid in the reaction tube to the connecting hole.

5. The kit according to claim 2, characterized in that: The second body is provided with an extraction hole along its axial direction. The extraction hole extends from the end surface of the second body to the extraction tube. The extraction tube is installed on the second body. The communication hole is connected to the extraction tube through the extraction hole.

6. The kit according to claim 5, characterized in that: The second body is provided with a first ventilation groove on its end surface close to the first body, the first ventilation groove is connected to the extraction hole, the abutment portion is provided with ventilation holes along the axial direction of the first body, and when the second body is in the first position, the ventilation holes are connected to the first ventilation groove.

7. The kit according to claim 6, characterized in that: The second body is provided with a second ventilation groove on the end surface close to the first body, and the second ventilation groove is connected to the waste liquid chamber. When the second body is in the second position, the ventilation hole is connected to the second ventilation groove.

8. The kit and automated extraction equipment according to claim 2, characterized in that: A first sealing groove and a plurality of second sealing grooves are provided at one end of the abutment portion close to the second body. The plurality of second sealing grooves are arranged at intervals along the circumference of the first body. The first sealing groove is connected to the connecting hole. A sealing ring is provided in the first sealing groove. A sealing gasket is provided in the second sealing groove.

9. The kit according to claim 1, characterized in that: The first magnet and the second magnet are respectively embedded in the side walls of the accommodating cavity.

10. An automated extraction device, characterized in that: The method comprises a driving device and a reagent kit as claimed in any one of claims 1 to 9, wherein the driving device can drive the second body to rotate relative to the first body.

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