Novel pleurotus nebrodensis culture medium formula and application thereof

Abstract

The invention relates to a novel pleurotus nebrodensis culture medium formula, in particular to a pleurotus nebrodensis culture medium formula which adopts licorice root residues as a pleurotus nebrodensis culture medium, and through scientific proportioning of the licorice root residues, cottonseed hulls, bran, cottonseed meal, corn flour and other raw materials, not only is the good effect of the licorice root residues brought into full play, but also the problem of insufficient carbon source supply when the licorice root residues serve as a main culture medium is solved. And through special proportioning of the raw materials and the auxiliary materials, the common problems of poor mycelial growth and high fungal contamination rate in the cultivation process are avoided. The novel pleurotus nebrodensis culture medium formula provided by the invention has a good application effect in pleurotus nebrodensis culture, and is of great significance in promoting sustainable development of the pleurotus nebrodensis industry.

Description

Technical Field

[0001] The invention relates to the technical field of microbial strains and applications thereof, and specifically to the technical field of application of a Pleurotus nebrodensis cultivation substrate. Background Art

[0002] Bailing mushroom is a rare edible fungus that has developed rapidly in recent years. It is named for its white color and similar shape to Ganoderma lucidum. The fruiting body of Bailing mushroom is tender and delicious, rich in protein, carbohydrates, fat, cellulose and various amino acids. In addition, Bailing mushroom is rich in vitamins and minerals, which have the effect of enhancing immunity and regulating physiological balance. Bailing mushroom is not only of high value in terms of food, but also shows significant potential in terms of medicine. Studies have shown that Bailing mushroom extract has multiple pharmacological effects such as anti-tumor and antioxidant. In particular, its polysaccharide component, which is considered to be the main active ingredient, has a significant effect on enhancing immune function and anti-tumor. In terms of the market, Bailing mushroom is highly favored for its high nutritional value and medicinal value. The cultivation process of Bailing mushroom is divided into three stages: spawning, post-ripening and fruiting, which requires strict control of temperature, humidity and light intensity. Although Bailing mushroom has high economic benefits and market prospects, although the current artificial cultivation technology has made certain progress, there are still some technical problems in actual operation, such as poor mycelium growth, high bag contamination rate and severe burn. Therefore, the future development direction should include improving cultivation technology and increasing bio-utilization efficiency. However, to achieve the sustainable development of the Pleurotus eryngii industry, it is necessary to continuously explore and optimize cultivation technology.

[0003] Licorice residue refers to the residue left after the effective ingredients of licorice are extracted. Its utilization value and recycling methods have received extensive attention in recent years. Licorice residue contains rich nutrients and bioactive substances, such as crude protein, crude fiber, crude fat, flavonoids and polysaccharides, which have multiple potential application values. However, the total organic matter contained in a single licorice residue is often not enough for growth, which means that its nutrient content may not be enough to support the comprehensive growth needs of mushrooms.

[0004] In the prior art, the Chinese invention patent with application number CN201711156623.8 discloses a Pleurotus eryngii matrix, which uses 48%-55% cottonseed hulls, 20%-30% corn cobs, 4%-9% sawdust, 8%-15% bran, 3%-6% corn flour, 1%-3% gypsum, and 0.1%-1.5% superphosphate. The technical solution of "cottonseed hulls can be used as the main material of the cultivation matrix" is disclosed. The Chinese invention patent with application number CN201110154463.X discloses a method for cultivating Pleurotus eryngii, and discloses a technical solution of "corn cobs as the main material of the cultivation matrix" with 60%-70% corn cobs; 10%-20% cottonseed hulls; 15%-18% bran; 1% stone powder; 1% sugar; and 1% distiller's grains. There is no related report on using licorice residue to cultivate Pleurotus eryngii in the related cultivation substrates reported in the prior art. The technical problem that the total organic matter of the single licorice residue used to prepare the new type of Pleurotus eryngii cultivation is often insufficient for growth and the nutrient content may not be sufficient to support the comprehensive growth needs of the mushroom needs to be solved. Summary of the invention

[0005] In view of the fact that there is no related report on using licorice residue to cultivate Pleurotus eryngii in the related cultivation substrates reported in the prior art, the technical problem that the total organic matter contained in the single licorice residue in the preparation of the new type of Pleurotus eryngii cultivation by directly using licorice residue is often not enough for growth, and the nutrient content may not be enough to support the overall growth needs of the mushroom is solved. The present invention aims to provide a new type of cultivation substrate formula for Pleurotus eryngii, specifically to use licorice residue as the cultivation substrate for Pleurotus eryngii, and by compounding raw materials such as cottonseed hulls, bran, cottonseed meal, corn flour, etc., while maximizing the good effects of licorice residue, solve the problem that licorice residue as the main cultivation substrate cannot provide a good total carbon source, and through the special ratio between raw and auxiliary materials, the problems of poor mycelium growth and high fungal contamination rate during the cultivation process are avoided. The new type of cultivation substrate formula for Pleurotus eryngii provided by the present invention has a good application effect in Pleurotus eryngii cultivation, and has a good application prospect for the sustainable development of Pleurotus eryngii resources.

[0006] In order to achieve the above technical effects, the present invention is implemented through the following technical solutions: The present application provides a novel cultivation matrix formula for Pleurotus nebrodensis, wherein the matrix comprises: 30%-70% licorice residue, 30%-70% cottonseed hull, 10% bran, 5% cottonseed meal, 5% corn flour, 0.5% nitrogen, phosphorus and potassium, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.3% potassium dihydrogen phosphate, 60-70% water content, and pH 7-8.

[0007] Preferably, the matrix comprises: 65% licorice residue, 25% cottonseed hull, 5% bran, 0.7% cottonseed meal, 3% corn flour, 0.5% nitrogen, phosphorus and potassium, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.3% potassium dihydrogen phosphate, 60-70% water content, and pH 7-8.

[0008] The culture medium is suitable for the cultivation of Pleurotus nebrodensis P1.x0008 or P1.x0009 varieties.

[0009] Furthermore, the present application also provides a method for cultivating Pleurotus nebrodensis, wherein the method comprises the following steps: (1) The new cultivation medium of Pleurotus nebrodensis is all grown in bags. The cultivation bags are made of high-density polyethylene or polypropylene film with specifications of 13-18 cm × 23-38 cm × 0.0025-0.004 cm. The pH value of the medium is controlled between 9-9.5. The medium is evenly loaded into the bag, with each bag containing 0.95-1.05 kg of medium. The culture medium is compacted to ensure moderate compactness, then the ring is put on and the lid is buckled. A large space needs to be reserved in the upper part of the bag.

[0010] (2) Sterilize the bag prepared in step (1) with high pressure steam at 121°C for 2.5-3 hours, slowly cool and reduce the pressure, and reduce the temperature to 100°C within 40-44 minutes. After sterilization, wait until the temperature of the bag drops below 30°C and inoculate in time.

[0011] (3) When the temperature of the bag in step (2) drops to 30°C, open the bag in the inoculation room or inoculation box, and inoculate the bacteria from both ends or one end under sterile operation, with the inoculation amount being 10% of the matrix.

[0012] (4) Place the inoculated material strip in step (3) into the culture room with a light intensity of 50 to 200 lux; provide adequate ventilation to keep the air fresh; and control the relative humidity of the air at 70% to 90%.

[0013] Preferably, the moisture content of the substrate during the mycelium growth stage should be controlled between 60% and 65%, and the relative humidity of the air should be kept below 70%.

[0014] Preferably, the relative humidity of the air should be increased to 85% to 95% during the fruiting body differentiation and development stage.

[0015] Preferably, the relative humidity of the air is maintained between 80% and 95% during the budding and fruiting stages.

[0016] Preferably, the culture temperature is 21°C-25°C.

[0017] The present application also provides the application of the above-mentioned novel cultivation substrate formula of Pleurotus nebrodensis in the cultivation of Pleurotus nebrodensis.

[0018] The present application also provides the application of the novel cultivation method of Pleurotus nebrodensis in the cultivation of Pleurotus nebrodensis.

[0019] By implementing the above specific technical solutions provided by the present invention and implementing the content of the present invention, the following beneficial effects can be obtained: (1) The novel culture medium for Pleurotus nebrodensis provided in the present application has a very significant effect on the growth of Pleurotus nebrodensis by adopting a certain raw material ratio. Compared with the control group, the mycelium growth rate and mycelium growth potential are significantly improved. The reasonable matrix ratio and main material selection in the technical solution provided in the present application are of great significance for the growth matrix of Pleurotus nebrodensis.

[0020] (2) The method for cultivating Pleurotus nebrodensis provided in the present application, which comprises a combination of a slow cooling and decompression process during the high-pressure sterilization stage and related steps of segmented humidity control, adopts a specific new culture matrix for Pleurotus nebrodensis in combination with a specific culture method. Through the close coordination of each step, the quality of cultivation is ensured while ensuring a good growth rate of Pleurotus nebrodensis, and the contamination of the mushroom bags by foreign bacteria is significantly reduced.

[0021] (3) The best embodiment group provided in this application has the lowest contamination rate. The white mushroom culture medium provided in the prior art did not achieve the same good effect on the basis of combining the best culture method of this application. The number of contaminated mushroom bags during the culture process was significantly higher than that of the best embodiment group provided in this application. It shows that the cultivation method of white mushroom provided in this application has a good white mushroom cultivation effect compared with the prior art by closely coordinating each step based on the use of the above-mentioned specific white mushroom cultivation matrix. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Shown is the mycelium growth of different formulations.

[0023] Figure A shows the growth of Pleurotus eryngii P1.x0008; Figure B shows the growth of Pleurotus eryngii P1.x0009 DETAILED DESCRIPTION The present invention is described below with reference to the following examples, but the present invention is not limited to the following examples. All raw and auxiliary materials used in the present invention, as well as the selected bacterial strain culture methods are well known in the art, and the % involved in the present invention are all mass volume percentages, unless otherwise specified.

[0024] In order to better explain the present invention, the main contents of the present invention are further illustrated below in conjunction with specific examples, but the contents of the present invention are not limited to the following examples. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are all commercially available commodities.

[0025] The Pleurotus nebrodensis varieties P1.x0008 and P1.x0009 involved in this application are varieties domesticated in the wild in Xinjiang by our research group. They were disclosed in the paper "Comparative Screening Experiment of Wild Pleurotus nebrodensis Strains in Xinjiang" published on March 21, 2014, and are preserved in the Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences. The general public can contact the Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences to obtain them.

[0026] PDA medium: 200 g potato, 20 g glucose, 20 g agar powder, add water to 1000 mL, pH = 7.0, sterilize with high pressure steam at 115°C for 30 min.

[0027] Example 1: A new culture medium formula for Pleurotus nebrodensis The present application provides a novel cultivation matrix formula for Pleurotus nebrodensis, wherein the matrix comprises: 30%-70% liquorice residue, 30%-70% cottonseed hull, 10% bran, 5% cottonseed meal, 5% corn flour, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.5% and 0.3% potassium dihydrogen phosphate.

[0028] Embodiment 2: A novel cultivation method of Pleurotus nebrodensis Furthermore, the present application also provides a method for cultivating Pleurotus nebrodensis, wherein the method comprises the following steps: (1) The new cultivation medium of Pleurotus nebrodensis is all grown in bags. The cultivation bags are made of high-density polyethylene or polypropylene film with specifications of 13-18 cm × 23-38 cm × 0.0025-0.004 cm. The pH value of the medium is controlled between 9-9.5. The medium is evenly loaded into the bag, with each bag containing 0.95-1.05 kg of medium. The culture medium is compacted to ensure moderate compactness, then the ring is put on and the lid is buckled. A large space needs to be reserved in the upper part of the bag.

[0029] (2) The bag filled in step (1) is sterilized by high pressure steam at 121°C for 2.5-3 hours, and then slowly cooled and decompressed. After the sterilization is completed, inoculate the bag in time when the temperature of the bag drops below 30°C.

[0030] (3) When the temperature of the bag in step (2) drops to 30°C, open the bag in the inoculation room or inoculation box, and inoculate the bacteria from both ends or one end under sterile operation, with the inoculation amount being 10% of the matrix.

[0031] (4) Place the inoculated material strip in step (3) into the culture room with a light intensity of 50 to 200 lux; provide adequate ventilation to keep the air fresh; and control the relative humidity of the air at 70% to 90%.

[0032] Preferably, the moisture content of the substrate during the mycelium growth stage should be controlled between 60% and 65%, and the relative humidity of the air should be kept below 70%.

[0033] Preferably, the relative humidity of the air should be increased to 85% to 95% during the fruiting body differentiation and development stage.

[0034] Preferably, the relative humidity of the air is maintained between 80% and 95% during the budding and fruiting stages.

[0035] Preferably, the culture temperature is 21°C-25°C.

[0036] Example 3: Screening of a new culture medium formula for Pleurotus nebrodensis Weigh PDA powder according to the formula ratio, add the corresponding amount of distilled water, shake well, sterilize at 121℃ high pressure steam for 15min, and pour the culture medium into a plate with a diameter of 9cm after the culture medium solidifies.

[0037] The collected fruiting bodies of P1.x0008 and P1.x0009 strains of Pleurotus nebrodensis were subjected to tissue separation and culture. The method was as follows: the fruiting bodies of Pleurotus nebrodensis were cut longitudinally along the cap and the stipe on a clean bench, and then a piece of mushroom flesh the size of a soybean was taken out from the fruiting body at the junction of the cap and the stipe with sterilized tweezers and quickly transferred to the center of the PDA medium slope, and cultured in the dark at a constant temperature of 23°C. Then the mycelium was purified and cultured. The method was as follows: the tip mycelium was picked from the edge of the colony, transferred to the PDA medium slope, and cultured in the dark at a constant temperature of 23°C until the mycelium covered the slope, and this was repeated 3 times. The purified strains that covered the PDA medium slope were stored in a 4°C environment for later use.

[0038] The new cultivation substrate formula of Pleurotus nebrodensis was prepared by using raw materials of different proportions. The substrate formula and grouping are as follows: Experimental group I: A novel cultivation matrix formula for Pleurotus nebrodensis, the matrix comprising: 70% cottonseed hulls, 30% corn cobs, 10% bran, 5% cottonseed meal, 5% corn flour, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.5% and 0.3% potassium dihydrogen phosphate.

[0039] Experimental Group II: A novel cultivation matrix formula for Pleurotus nebrodensis, the matrix comprising: 70% cottonseed hulls, 30% liquorice residue, 10% bran, 5% cottonseed meal, 5% corn flour, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.5% and 0.3% potassium dihydrogen phosphate.

[0040] Experimental group III: A novel cultivation matrix formula for Pleurotus nebrodensis, the matrix comprising: 65% liquorice residue, 25% cottonseed hulls, 5% bran, 0.7% cottonseed meal, 3% corn flour, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.5% nitrogen, phosphorus, potassium and sulfur, and 0.3% potassium dihydrogen phosphate.

[0041] Test group IV: A novel cultivation matrix formula for Pleurotus nebrodensis, the matrix comprising: 35% liquorice residue, 35% cottonseed hulls, 30% corn cobs, 10% bran, 5% cottonseed meal, 5% corn flour, 0.5% nitrogen, phosphorus, potassium and sulfur, 0.5%, and 0.3% potassium dihydrogen phosphate.

[0042] Control CK group: a new type of cultivation matrix formula for Pleurotus nebrodensis, the matrix comprising: 91.7% cottonseed hulls, 5% corn flour, 1% sucrose, 1% gypsum powder, 1% lime powder, and 0.3% potassium dihydrogen phosphate.

[0043] Each group of new cultivation substrate formulas of Pleurotus nebrodensis were cultivated in bags. The cultivation bags were made of high-density polyethylene or polypropylene film with a thickness of 0.04-0.06 cm, 13-18 cm × 23-38 cm × 0.0025-0.004 cm. The pH value of the substrate was controlled between 9-9.5. When bagging, tie one end of the bag first, and evenly load the substrate into the bag, with 0.95-1.05 kg of substrate per bag; after the loaded material bags were sterilized by high pressure for 2-3 hours, they were slowly cooled and decompressed, and the temperature was reduced to 100°C within 40-44 minutes. After sterilization, the material bags were taken out and placed in the inoculation room for cooling; when the temperature of the material bags dropped to 30°C, the bag mouth was opened in the inoculation room or inoculation box, and the fungus was inoculated from both ends or one end under sterile operation, and the inoculation amount was 10% of the substrate. The inoculated material strips are placed in the culture room with a light intensity of 50 to 200 lux; proper ventilation is provided to keep the air fresh; and the relative humidity of the air is controlled at 70% to 90%.

[0044] The moisture content of the substrate should be controlled between 60% and 65% during the mycelium growth stage, and the relative humidity of the air should be kept below 70%. The relative humidity of the air should be increased to 85% to 95% during the fruiting body differentiation and development stage. The relative humidity of the air should be kept between 80% and 95% during the budding and fruiting stages. The culture temperature is 23°C.

[0045] Mycelial growth rate is an important indicator to measure the growth rate of fungi, which is usually calculated by measuring the growth length of mycelium in a certain period of time. The formula for calculating mycelial growth rate is as follows: Mycelium growth rate = growth days (d) / mycelium length (cm) Growth of mycelium: “+” weak, “++” average, “++++” strong, “++++++” relatively strong.

[0046] The growth test results are shown in the attached Figure 1 As shown in Table 1: Table 1: Growth rate of mycelium with different formulations

[0047] Based on the records in Table 1 above, the mycelial growth rate and mycelial growth potential of the test groups I, II and III provided in this application are significantly improved compared with the control group CK, among which the test group III has the best mycelial growth rate and mycelial growth potential. In the test group IV, the mycelial growth rate and mycelial growth potential are at the lowest level due to the compatibility of licorice residue, cottonseed hulls and corn cobs in a considerable proportion, indicating that the ratio of each raw material in the cultivation matrix of Pleurotus nebrodensis has a very significant effect on the growth of Pleurotus nebrodensis. Reasonable matrix ratio and main ingredient selection are of great significance for the growth matrix of Pleurotus nebrodensis.

[0048] Example 4: Screening of cultivation methods for Pleurotus eryngii Based on the records in the above-mentioned Examples 2 to 3, the substrate ratio provided by the Experiment III Group in Example 3 was selected to optimize the cultivation method of Pleurotus nebrodensis. The processing conditions of each group are as follows: Experimental group: the culture temperature was set at 21°C, and the rest of the treatment was the same as the cultivation method of Pleurotus nebrodensis described in Example 2. Experimental Group 2: The culture temperature was set at 22° C., and the rest of the treatments were the same as the cultivation method of Pleurotus nebrodensis described in Example 2.

[0049] Experimental group 3: The culture temperature was set at 23° C., and the rest of the treatments were the same as the cultivation method of Pleurotus nebrodensis recorded in Example 2.

[0050] Experimental group 4: The culture temperature was set at 24°C, and the rest of the treatments were the same as the cultivation method of Pleurotus nebrodensis recorded in Example 2.

[0051] Experimental Group 5: The culture temperature was set at 25° C., and the rest of the treatments were the same as the cultivation method of Pleurotus nebrodensis described in Example 2.

[0052] Experimental Group 6: After high pressure sterilization for 2-3 hours in step (2), the mixture was directly sterilized at normal pressure for 10-18 hours. After sterilization, the material bags were taken out and placed in the inoculation room for cooling. The rest of the treatment was the same as the cultivation method of Pleurotus nebrodensis described in Example 2.

[0053] Experimental Group 7: The relative humidity of the air was controlled at 70% to 90% during the mycelium growth stage, and the remaining treatments were the same as the cultivation method of Pleurotus nebrodensis recorded in Example 2.

[0054] Experimental Group 8: In the fruiting body differentiation and development stage, the relative humidity of the air was controlled at 70% to 90%, and the remaining treatments were the same as the cultivation method of Pleurotus nebrodensis recorded in Example 2.

[0055] Experimental group nine: wherein the relative humidity of the air was controlled at 70% to 90% during the budding and fruiting stages, and the remaining treatments were the same as the cultivation method of Pleurotus nebrodensis recorded in Example 2.

[0056] Each test group prepared 10 bags for cultivation, repeated 3 times, and cultivated to the fruiting stage to observe whether there was mold or contamination by foreign bacteria. The number of contaminated bags was counted, and the ratio of the number of contaminated bags to the number of test bags in each group was calculated. The statistical results are shown in Table 2: Table 2: Pollution statistics

[0057] Based on the records in Table 2 above, the contamination rates of Experiments 1 to 5 provided in this application, which contain the slow cooling and decompression process in the high-pressure sterilization stage, and the related steps of segmented humidity control, are relatively low. Among them, in the different temperature cultivation processes, the contamination rate of Experiment 3, in which the cultivation temperature is set to 23°C, is the lowest, which has a significant effect. The contamination rate of Experiment 6 was significantly increased due to the lack of slow cooling and decompression process in the high-pressure sterilization stage; the contamination rate of Experiments 7 to 9 was also significantly increased in some stages because effective humidity control was not performed. The statistical results in Table 2 show that the cultivation method of Pleurotus eryngii provided in this application, based on the use of the above-mentioned specific cultivation substrate of Pleurotus eryngii, through the close cooperation of each step, ensures the quality of cultivation on the basis of ensuring the good growth rate of Pleurotus eryngii, and significantly reduces the contamination of the mushroom bags by miscellaneous bacteria.

[0058] Example 5: Cultivation effect comparison Based on the records in the above-mentioned Examples 3 and 4, the substrate ratio provided by the Experimental Group III in Example 3 was selected, combined with the best culture method provided by the Experimental Group III in Example 4 as the best embodiment group, and the culture effect was compared with the relevant Pleurotus nebrodensis substrate provided in the prior art combined with the best culture method provided in this application.

[0059] Comparative Example 1: A Pleurotus eryngii matrix disclosed in the prior art in the Chinese invention patent with application number CN201711156623.8 is made of the following raw materials in weight percentage: 52% cottonseed hulls, 25% corn cobs, 6% sawdust, 10% bran, 5% corn flour, 1.5% gypsum, and 0.5% superphosphate. Combined with the optimal culture method provided by the three test groups in Example 4, it is used as a comparative example group.

[0060] Comparative Example 2: A Pleurotus eryngii matrix disclosed in the Chinese invention patent with application number CN201711156623.8 in the prior art is made of the following raw materials in weight percentage: 50% cottonseed hulls, 27% corn cobs, 8% sawdust, 8% bran, 4% corn flour, 2% gypsum, and 1% superphosphate, combined with the optimal culture method provided by the third test group in Example 4, as the second comparative example group.

[0061] Comparative Example 3: A Pleurotus eryngii matrix disclosed in the prior art in the Chinese invention patent with application number CN201711156623.8 is made of the following raw materials in weight percentage: 48% cottonseed hulls, 29% corn cobs, 7% sawdust, 6% bran, 6% corn flour, 2.5% gypsum, and 1.5% superphosphate. Combined with the best culture method provided by the three test groups in Example 4, it is used as the third comparative example.

[0062] Comparative Example 4: A method for cultivating Pleurotus eryngii disclosed in the Chinese invention patent with application number CN201110154463.X, the raw material composition and weight percentage of the mushroom stick are as follows: 60% corn cob, 20% cottonseed hull, 18% bran, 1% sesame, 1% sugar, and 1% distiller's grains. Combined with the best cultivation method provided by the third group of experiments in Example 4, the fourth group of comparative examples was used.

[0063] Comparative Example 5: A method for cultivating Pleurotus eryngii disclosed in the Chinese invention patent with application number CN201110154463.X, the raw material composition and weight percentage of the mushroom stick are as follows: 70% corn cob, 15% cottonseed hull, 14% bran, 1% sesame, 1% sugar, and 1% distiller's grains. Combined with the best cultivation method provided by the third group of experiments in Example 4, the fifth group of comparative examples was used.

[0064] Each group prepared 10 bags for cultivation, repeated 3 times, and cultivated to the fruiting stage to observe whether there was mold or contamination by foreign bacteria. The number of contaminated bags was counted, and the ratio of the number of contaminated bags to the number of experimental bags in each group was calculated. The statistical results are shown in Table 3: Table 3: Pollution statistics

[0065] Based on the records in Table 3 above, the contamination rate of the best embodiment group provided in this application is the lowest. The white mushroom culture medium provided in the prior art did not achieve the same good effect on the basis of combining the best culture method of this application, and the number of contaminated mushroom bags during the mushroom bag culture process was significantly higher than that of the best embodiment group provided in this application. The statistical results in Table 3 show that the cultivation method of white mushroom provided in this application, based on the use of the above-mentioned specific white mushroom cultivation matrix, has a good white mushroom cultivation effect compared with the prior art through the close cooperation of each step.

[0066] The embodiments described above are only some embodiments of the present invention, not all embodiments. The detailed description of the embodiments of the present invention is not intended to limit the scope of the invention claimed for protection, but only represents selected embodiments of the present invention. All other embodiments obtained without creative work and related deductions and substitutions made by ordinary technicians in the field under the conditions of the concept of the present invention belong to the scope of protection of the present invention.

Claims

1. A novel cultivation medium formula for Pleurotus nebrodensis, characterized in that: The matrix comprises: 30%-70% of liquorice residue, 30%-70% of cottonseed hull, 10% of bran, 5% of cottonseed meal, 5% of corn flour, 0.5% of nitrogen, phosphorus and potassium, 0.5% of nitrogen, phosphorus, potassium and sulfur, 0.3% of potassium dihydrogen phosphate, 60%-70% of water content, and pH 7-8.

2. A novel cultivation medium formula for Pleurotus nebrodensis according to claim 1, characterized in that: The matrix comprises: 65% of liquorice residue, 25% of cottonseed hull, 5% of bran, 0.7% of cottonseed meal, 3% of corn flour, 0.5% of nitrogen, phosphorus and potassium, 0.5% of nitrogen, phosphorus, potassium and sulfur, 0.3% of potassium dihydrogen phosphate, 60-70% of water content, and pH 7-8.

3. A novel cultivation medium formula for Pleurotus nebrodensis according to claim 1, characterized in that: The culture medium is suitable for the cultivation of Pleurotus nebrodensis P1.x0008 or P1.x0009 varieties.

4. A novel cultivation method of Pleurotus nebrodensis, characterized in that: The novel cultivation substrate formula of Pleurotus nebrodensis as claimed in claim 1 is used, and the specific cultivation method is as follows: (1) The new cultivation medium of Pleurotus nebrodensis is all grown in bags. The cultivation bags are made of high-density polyethylene or polypropylene film with specifications of 13-18 cm × 23-38 cm × 0.0025-0.004 cm. The pH value of the medium is controlled between 9-9.

5. The medium is evenly loaded into the bag, with each bag containing 0.95-1.05 kg of medium. The culture medium is compacted to ensure moderate compactness, then the ring is put on and the lid is buckled. A large space needs to be reserved in the upper part of the bag. (2) The bag prepared in step (1) is sterilized by high pressure steam at 121°C for 2.5-3 hours, and then slowly cooled and decompressed to reduce the temperature to 100°C within 40-44 minutes. After sterilization, the bag is inoculated in time when the temperature of the bag drops below 30°C. (3) When the temperature of the bag in step (2) drops to 30°C, open the bag in the inoculation room or inoculation box, and inoculate the bacteria from both ends or one end under sterile operation, with the inoculation amount being 10% of the matrix. (4) Place the inoculated material strip in step (3) into the culture room with a light intensity of 50 to 200 lux; provide adequate ventilation to keep the air fresh; and control the relative humidity of the air at 70% to 90%.

5. A novel cultivation method of Pleurotus nebrodensis as claimed in claim 4, characterized in that: The moisture content of the substrate during the mycelium growth stage should be controlled between 60% and 65%, and the relative humidity of the air should be kept below 70%.

6. The novel cultivation method of Pleurotus nebrodensis according to claim 4, characterized in that: During the fruiting body differentiation and development stage, the relative humidity of the air should be increased to 85% to 95%.

7. The novel cultivation method of Pleurotus nebrodensis according to claim 4, characterized in that: During the bud induction and mushroom fruiting stages, the relative humidity of the air should be maintained between 80% and 95%.

8. The novel cultivation method of Pleurotus nebrodensis according to claim 4, characterized in that: The culture temperature is 21℃-25℃.

9. Use of a new cultivation substrate formula of Pleurotus nebrodensis as claimed in any one of claims 1 to 3 in the cultivation of Pleurotus nebrodensis.

10. Use of the novel cultivation method of Pleurotus nebrodensis according to any one of claims 4 to 8 in the cultivation of Pleurotus nebrodensis.

Images