A plant sunscreen synergistic composition suitable for sensitive skin and its application

Abstract

The invention discloses a kind of plant sunscreen synergistic composition and application thereof, belong to the field of cosmetics technology; The plant sunscreen synergistic composition provided by the present invention includes the following components in parts by mass: 0.1-1 parts of Cimicifuga extract, 0.3-1 parts of Eucommia leaf extract, 1-3 parts of Rambutan peel extract, 5-10 parts of Cyperus root extract. The present invention is compounded by selecting the components of appropriate mass ratio, and the obtained composition has a good effect of absorbing ultraviolet rays, and also has excellent anti-inflammatory and soothing effect. Moreover, when subsequently added into sunscreen products, it can effectively stabilize the physical sunscreen and chemical sunscreen in sunscreen lotion, and can also enhance the sunscreen effect of physical sunscreen and chemical sunscreen, improve the durability of sunscreen products, and can enhance the repair of sunscreen products to skin after sun exposure.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and in particular relates to a plant sunscreen synergistic composition and application thereof. Background Art

[0002] Sunscreen products reduce skin damage from UV rays by adding sunscreens that reflect or absorb UV radiation. Sunscreen products require a large amount of physical and chemical sunscreens to provide adequate protection. However, excessive amounts of sunscreen can cause varying degrees of damage to the skin and are unsuitable for people with sensitive skin.

[0003] At the same time, the existing sun protection function is relatively simple. Without sufficient sun protection, the skin will be prone to photoburn and photosensitivity, leading to symptoms such as redness and inflammation of the skin; further use of sun protection products that add a large amount of physical sunscreens and chemical sunscreens will further aggravate skin inflammation, allergies and other reactions. Summary of the Invention

[0004] The purpose of the present invention is to overcome the shortcomings of the above-mentioned prior art and provide a plant sunscreen synergistic composition and its application which has excellent ultraviolet absorption effect and anti-inflammatory soothing effect, can effectively enhance the sun protection ability and stability of physical sunscreens and chemical sunscreens, and can repair sensitive skin after sun exposure.

[0005] To achieve the above objectives, in a first aspect of the present invention, the present invention provides a plant sunscreen synergistic composition, which comprises the following components in parts by weight:

[0006] 0.1-1 parts of Cimicifuga extract, 0.3-1 parts of Eucommia ulmoides leaf extract, 1-3 parts of rambutan peel extract, and 5-10 parts of Cyperus rotundus root extract.

[0007] The present invention selects components in a suitable mass ratio, specifically Cimicifuga heracleifolia extract, Eucommia ulmoides leaf extract, Rambutan leaf peel extract, and Cyperus rotundus root extract. The four ingredients have an excellent compounding effect, and on the basis of achieving good ultraviolet absorption ability under synergistic effect, also have an excellent anti-inflammatory effect. Moreover, when the four ingredients are subsequently added to a sunscreen product, the sunscreen effects of the physical sunscreen agents and chemical sunscreen agents in the original sunscreen product can be effectively enhanced, the durability of the sunscreen product can be improved, and the sunscreen product can be enhanced to repair the skin after sun exposure.

[0008] Specifically, the present invention found that the cycloaltinane-type triterpene compounds contained in the Cimicifuga extract are its characteristic active ingredients, which have antibacterial and anti-inflammatory effects. In addition, the cimicifuga in Cimicifuga can regulate the expression of some genes and inhibit the pathological changes of inflammatory cells, showing anti-inflammatory and anti-allergic effects. The cinnamic acid derivatives therein can also protect the basement membrane from inflammatory damage by inhibiting the activity of neutrophil elastase; for sun protection, the active substances in the Cimicifuga extract, such as ferulic acid itself, have a certain absorption capacity for ultraviolet rays, and can also have a good synergistic effect on physical sunscreens, especially titanium dioxide and zinc oxide. The flavonoids and phenolic acids in Eucommia leaf extract not only possess excellent antioxidant and anti-inflammatory properties but also possess a certain degree of UV absorption. Furthermore, Eucommia leaf extract is rich in chlorogenic acid, which not only has excellent UV absorption capacity itself but also has a strong synergistic effect on chemical sunscreens, especially diethylaminohydroxybenzoyl hexyl benzoate. Furthermore, iridoids such as aucubin, eucommia alcohol, and geniposide in Eucommia leaf extract also possess anti-inflammatory and analgesic effects. Rambutan peel extract contains ellagic acid and quercetin. These ingredients, in addition to their inherent UV absorption capacity, can minimize the photolysis of chemical sunscreens, especially ethylhexyl methoxycinnamate, thereby preventing the loss of their sunscreen effectiveness due to photolysis and enhancing their long-lasting sun protection. Furthermore, the polyphenols in rambutan peel can increase the synthesis of phospholipids and ceramides in the epidermis, strengthening the stratum corneum's barrier function against dehydration and achieving a good repair and soothing effect. In addition to its ability to absorb ultraviolet rays, Cyperus rotundus root extract can also alleviate UV-induced inflammation in epidermal keratinocytes and promote their metabolism, resulting in excellent soothing and repairing effects. Furthermore, through extensive inventive experiments, the present invention has discovered that compounding the four components in a specific mass ratio creates a synergistic effect, resulting in a product with superior ultraviolet absorption and anti-inflammatory effects.

[0009] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the plant sunscreen synergistic composition comprises the following components in parts by mass: 0.5-0.6 parts of Cimicifuga extract, 0.4-0.5 parts of Eucommia ulmoides leaf extract, 1.5-2 parts of rambutan peel extract, and 7-8 parts of Cyperus rotundus root extract.

[0010] The present invention has found that the mass parts of the components in the plant sunscreen synergistic composition will affect the comprehensive performance of the composition. When the mass parts of the components in the plant sunscreen synergistic composition are further selected to be within the above-mentioned range, the obtained composition has better ultraviolet absorption capacity and better anti-inflammatory and soothing effects. When applied to sunscreen products, the sunscreen ability of the sunscreen products is more significantly improved, the sunscreen lasting power is stronger, and the after-sun repair effect is also better.

[0011] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the plant sunscreen synergistic composition, the mass percentage of the rambutan peel extract is 11-22%.

[0012] Illustratively, in the plant sunscreen synergistic composition, the mass percentage of rambutan peel extract can be any point value between 11-17% or any two point range values, such as 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, etc.

[0013] The present invention has found that the mass percentage of rambutan peel extract in the plant sunscreen synergistic composition not only affects the ultraviolet absorption ability of the composition, but also affects the anti-inflammatory and soothing effects of the composition. When the mass percentage of rambutan peel extract is further selected to be within the above range, the comprehensive effect of the obtained plant sunscreen synergistic composition is better, and when used in the preparation of sunscreen products, the obtained sunscreen product has a stronger ability to repair sun-exposed skin, and has better sunscreen ability and sunscreen durability.

[0014] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the mass percentage of chlorogenic acid in the Eucommia ulmoides leaf extract is ≥5%.

[0015] It should be noted that the mass percentage of chlorogenic acid in the Eucommia ulmoides leaf extract was obtained by high performance liquid chromatography (HPLC) as described in the content determination of Eucommia ulmoides leaf in the 2020 edition of the Chinese Pharmacopoeia. The test method is as follows:

[0016] Chromatographic conditions: Octadecylsilane bonded silica gel is used as the filler; acetonitrile-0.4% phosphoric acid solution (13:87) is used as the mobile phase; the detection wavelength is 327nm. Preparation of reference substance: Take an appropriate amount of chlorogenic acid reference substance, accurately weigh it, place it in a brown volumetric flask, and add 50% methanol to make a solution containing 50μg per 1ml. Preparation of test sample: Take about 1g of the test sample powder, accurately weigh it, place it in a stoppered conical flask, accurately add 25ml of 50% methanol, weigh the weight, heat and reflux for 30 minutes, cool, weigh the weight again, make up the lost weight with 50% methanol, shake well, filter, and take the filtrate. Determination: Accurately aspirate 10μl of the reference solution and the test solution respectively, inject them into the liquid chromatograph, and determine the result.

[0017] Exemplarily, the mass percentage of chlorogenic acid in the Eucommia leaf extract can be any point value ≥5% or any two-point range value, such as 5-10%, 5-15%, 5-20%, 2-25%, etc., or can be 5%, 7%, 9%, 10%, 12%, 14%, 16%, 18%, 20%, 22%, 25%, etc.

[0018] The present invention has found that when the mass percentage of chlorogenic acid in the Eucommia ulmoides leaf extract is further selected to be within the above-mentioned range, it can not only further enhance the ultraviolet absorption ability of the composition, but also, when subsequently added to the preparation of sunscreen products, can better enhance the synergy of the chemical sunscreen agents in the sunscreen products, thereby effectively improving the sunscreen ability and sunscreen durability of the sunscreen products.

[0019] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the preparation method of the Cimicifuga extract includes the following steps: taking Cimicifuga and adding it to an ethanol aqueous solution for microwave extraction, filtering after the extraction is completed, collecting the filtrate and concentrating and drying it to obtain the Cimicifuga extract.

[0020] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cimicifuga extract, the mass percentage of ethanol in the ethanol aqueous solution is 60-80%, and the mass volume ratio of Cimicifuga to the ethanol aqueous solution is 1g: (15-25)mL.

[0021] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cimicifuga extract, the power of microwave extraction is 380-390W and the time is 80-100s.

[0022] Preferably, the method further comprises the steps of drying, crushing and screening before adding the Cimicifuga to the ethanol aqueous solution.

[0023] The present invention has found that the preparation method of Cimicifuga extract will affect the content and type of active ingredients in the Cimicifuga extract, and thus affect the interaction between it and other components. When the preparation method of Cimicifuga extract is further selected as the method described in the present invention, the obtained compounding effect of Cimicifuga extract and other components is better, the obtained plant sunscreen synergistic composition has a better comprehensive effect, and when subsequently applied to sunscreen products, the obtained sunscreen product also has a better effect.

[0024] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, the preparation method of the Cyperus rotundus root extract includes the following steps: taking the Cyperus rotundus root, crushing it and adding it to an ethanol aqueous solution, heating and extracting it, filtering and concentrating it after the extraction is completed to obtain the Cyperus rotundus root extract.

[0025] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cyperus rotundus root extract, the mass percentage of ethanol in the ethanol aqueous solution is 45-55%, and the mass volume ratio of Cyperus rotundus root and ethanol aqueous solution is 1g: (3-7)mL.

[0026] As a preferred embodiment of the plant sunscreen synergistic composition of the present invention, in the preparation method of the Cyperus rotundus root extract, the heating extraction temperature is 65-75° C. and the time is 1-2 hours.

[0027] Preferably, the method further comprises the steps of drying, crushing and screening before adding the Cyperus rotundus root to the ethanol aqueous solution.

[0028] The present invention has found that the preparation method of the Cyperus rotundus root extract will affect the content and type of active ingredients in the Cyperus rotundus root extract, and thus affect the interaction between the active ingredients and other components. When the preparation method of the Cyperus rotundus root extract is further selected as the method described in the present invention, the obtained compounding effect of the Cyperus rotundus root extract and other components is better, the obtained plant sunscreen synergistic composition has better soothing effect, and when subsequently used in the preparation of sunscreen products, the obtained sunscreen products have better sun protection effect and after-sun repair effect.

[0029] In the second aspect of the present invention, the present invention provides an application of the plant sunscreen synergistic composition in the preparation of sunscreen products.

[0030] The plant sunscreen synergistic composition provided by the present invention has good ultraviolet absorption ability and anti-inflammatory effect. When preparing sunscreen products, it can also effectively increase the sunscreen ability of physical sunscreens and chemical sunscreens in sunscreen products and improve the stability of chemical sunscreens. Therefore, it can be widely used in the preparation of sunscreen products. The obtained sunscreen products not only have good and long-lasting sunscreen ability, but also have excellent after-sun repair effect.

[0031] In the third aspect of the present invention, the present invention provides a sunscreen lotion, which includes the following components in mass percentage: 10-15% of the plant sunscreen synergistic composition of the present invention, 5-7% physical sunscreen, 10-15% chemical sunscreen, 10-15% emollient, 2-3% film former, 5-12% moisturizer, 0.1-0.5% thickener, 0.5-2% preservative, 1-5% emulsifier, and the balance water.

[0032] Research in the present invention has found that by adding the plant sunscreen synergistic composition of the present invention to the sunscreen lotion provided by the present invention, the plant sunscreen synergistic composition and the physical sunscreen and chemical sunscreen have good synergistic effects, which can not only effectively improve the sunscreen ability of the sunscreen lotion and enhance the long-lasting sunscreen ability of the sunscreen lotion, but also effectively repair the skin after sun exposure, achieving an excellent effect of soothing the skin after sun exposure.

[0033] As a preferred embodiment of the sunscreen lotion of the present invention, the physical sunscreen includes zinc oxide and titanium dioxide; the chemical sunscreen includes diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine and ethylhexyl triazone.

[0034] The present invention has found that when physical sunscreens and chemical sunscreens are further selected as the above-mentioned types, the plant sunscreen synergistic composition has a better synergistic effect on the above-mentioned physical sunscreens, as well as a better synergistic and stabilizing effect on the above-mentioned chemical sunscreens. Therefore, not only is the sunscreen ability and sunscreen durability of the product more significantly improved, but the repair effect of the resulting sunscreen product is also better.

[0035] As a preferred embodiment of the sunscreen lotion of the present invention, the emollient includes at least one of dicaprylyl carbonate and diisopropyl sebacate.

[0036] As a preferred embodiment of the sunscreen lotion of the present invention, the film-forming agent includes at least one of trimethylsiloxysilicate and cyclopentasiloxane.

[0037] As a preferred embodiment of the sunscreen lotion of the present invention, the moisturizing agent includes at least one of glycerin, 1,3-butylene glycol, and 1,2-pentanediol.

[0038] As a preferred embodiment of the sunscreen lotion of the present invention, the thickener includes at least one of xanthan gum, carbomer, and sodium polyacrylate.

[0039] As a preferred embodiment of the sunscreen lotion of the present invention, the preservative includes at least one of p-hydroxyacetophenone and hexylene glycol.

[0040] As a preferred embodiment of the sunscreen lotion of the present invention, the emulsifier includes at least one of polyglyceryl-10 oleate, polysorbate 60, and polyglyceryl-3 diisostearate.

[0041] Compared with the prior art, the present invention has the following beneficial effects:

[0042] The present invention selects components in appropriate mass ratios, specifically Cimicifuga heracleifolia extract, Eucommia barbata leaf extract, Rambutan leaf peel extract, and Cyperus rotundus root extract. These four ingredients have excellent compounding effects, resulting in a composition that has good ultraviolet absorption and excellent anti-inflammatory and soothing effects. Furthermore, when subsequently added to sunscreen products, it can effectively stabilize the physical and chemical sunscreens in sunscreen lotions, enhance the sunscreen effect of the physical and chemical sunscreens, improve the durability of the sunscreen product, and enhance the sunscreen product's ability to repair sun-exposed skin. BRIEF DESCRIPTION OF THE DRAWINGS

[0043] Figure 1 This is a before-and-after comparison of a volunteer using the sunscreen lotion in Application Example 1 in Effect Example 2;

[0044] Figure 2 This is a before-and-after comparison of volunteers using the sunscreen lotion in Comparative Application Example 1 in Effect Example 2;

[0045] Figure 3 This is a before-and-after comparison of the volunteers using the sunscreen lotion in the blank example in Effect Example 2. DETAILED DESCRIPTION

[0046] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0047] Unless otherwise specified, the reagents, methods, and equipment used in the present invention are all conventional reagents, methods, and equipment in the art; and unless otherwise specified, the raw materials used in parallel experiments are from the same batch of raw materials.

[0048] Cimicifuga extract 1: Homemade, the preparation method includes the following steps:

[0049] Commercially available Cimicifuga foetida was dried, crushed, and passed through an 80-mesh sieve. The sieve residue was collected and added to a 70% (mass-volume ratio of sieve residue to ethanol solution: 1 g: 20 mL) ethanol solution, and microwave irradiated at 385 W for 90 s. After microwave extraction, the filtrate was collected and concentrated at 45°C, and then dried in a vacuum drying oven at 60°C to obtain Cimicifuga foetida extract 1.

[0050] Cimicifuga extract 2: Homemade, the preparation method includes the following steps:

[0051] Commercially available Cimicifuga foetida was dried, crushed, and passed through an 80-mesh sieve. The sieve residue was collected and added to a 30% (mass-volume) ethanol solution (the mass-volume ratio of the sieve residue to the ethanol solution was 1 g:20 mL). The mixture was microwave-irradiated at 400 W for 110 s. After microwave extraction, the mixture was filtered, the filtrate was collected, concentrated at 45°C, and then dried in a vacuum drying oven at 60°C to obtain Cimicifuga foetida extract 2.

[0052] Cimicifuga extract 3: Homemade, the preparation method includes the following steps:

[0053] Commercially available Cimicifuga foetida was dried, crushed, and passed through an 80-mesh sieve. The sieve residue was collected and added to a 70% (mass-volume) ethanol solution (the mass-volume ratio of the sieve residue to the ethanol solution was 1 g:20 mL). Ultrasonic extraction was performed at 80W and 30°C for 30 minutes. After the ultrasonic extraction, the mixture was filtered, and the filtrate was collected and concentrated at 45°C. It was then dried in a vacuum drying oven at 60°C to obtain Cimicifuga foetida extract 3.

[0054] Eucommia ulmoides leaf extract 1: purchased from Changsha Shanghe Biotechnology Co., Ltd., with a chlorogenic acid content of 5%;

[0055] Eucommia ulmoides leaf extract 2: homemade, take commercially available Eucommia ulmoides leaves, dry them, and crush them through a 60-mesh sieve. Collect the sieve residue and add water (the mass volume ratio of the sieve residue and water is 1 g:15 mL) and stir at 90°C for 30 min. After extraction, filter, collect the filtrate and concentrate it at 60°C, and then dry it in a vacuum drying oven at 60°C to obtain Eucommia ulmoides leaf extract 2. The mass percentage of chlorogenic acid was measured to be 2%.

[0056] Rambutan peel extract: purchased from BASF under the trade name Nephydrat TM BC10061;

[0057] Cyperus rotundus root extract 1: Homemade, the preparation method includes the following steps:

[0058] The commercially available Cyperus rotundus root was dried, crushed, and passed through an 80-mesh sieve. The sieve residue was collected and added to a 50% (mass-volume ratio of sieve residue to ethanol solution: 1 g: 5 mL) ethanol solution, stirred at 70°C for 1.5 h. After the heating extraction was completed, the mixture was filtered, and the filtrate was collected and concentrated at 45°C to obtain Cyperus rotundus root extract 1.

[0059] Cyperus rotundus root extract 2: Homemade, the preparation method includes the following steps:

[0060] The commercially available Cyperus rotundus root was dried, crushed and passed through an 80-mesh sieve. The sieve residue was collected and added to an 80% ethanol aqueous solution (the mass volume ratio of the sieve residue and the ethanol aqueous solution was 1 g: 5 mL) and stirred at 40°C for 1.5 h. After the heating extraction was completed, the mixture was filtered, the filtrate was collected and concentrated at 45°C to obtain Cyperus rotundus root extract 2.

[0061] Examples 1-8 and Comparative Examples 1-6

[0062] The embodiments and comparative examples of the present invention provide a plant sunscreen synergistic composition, the components (parts by mass) of the composition are shown in Table 1;

[0063] Table 1

[0064]

[0065]

[0066] The preparation method of the plant sunscreen enhancement composition provided in Example 1 is: mixing the components evenly to obtain the plant sunscreen enhancement composition.

[0067] The preparation methods of the plant sunscreen enhancement compositions provided in Examples 2-8 and Comparative Examples 1-6 are consistent with that in Example 1.

[0068] Comparative Example 7

[0069] The comparative example of the present invention provides a plant sunscreen enhancement composition. The only difference between the plant antiperspirant and sunscreen repair composition and Example 1 is that the Cimicifuga racemosa root extract is used instead of the Cimicifuga Extract 1.

[0070] Comparative Example 8

[0071] The comparative example of the present invention provides a plant sunscreen enhancement composition. The only difference between the plant antiperspirant and sunscreen repair composition and Example 1 is that the Eucommia bark extract is used instead of the Eucommia leaf extract 1.

[0072] Comparative Example 9

[0073] The comparative example of the present invention provides a plant sunscreen enhancement composition. The only difference between the plant anti-perspirant and sunscreen repair composition and Example 1 is that the rambutan peel extract is replaced by litchi peel extract.

[0074] Comparative Example 10

[0075] The comparative example of the present invention provides a plant sunscreen enhancement composition. The only difference between the plant antiperspirant and sunscreen repair composition and Example 1 is that the Cyperus rotundus root extract 1 is replaced by the Cyperus rotundus root extract.

[0076] Application Examples 1-12, Comparative Application Examples 1-10

[0077] The application examples of the present invention and the comparative application examples provide a sunscreen lotion, the components (mass percentage) of which are shown in Table 2; wherein the plant sunscreen synergistic compositions used in Application Examples 1-8 are respectively the plant sunscreen synergistic compositions prepared in Examples 1-8, and the plant sunscreen synergistic compositions used in Comparative Application Examples 1-10 are respectively the plant sunscreen synergistic compositions prepared in Comparative Examples 1-10; the plant sunscreen synergistic compositions used in Application Examples 9-12 are the plant sunscreen synergistic compositions prepared in Example 1;

[0078] In addition, a blank application example is set, wherein the only difference between the blank application example and application example 1 is that the plant sunscreen synergistic composition is not added;

[0079] Table 2

[0080]

[0081] The preparation method of the sunscreen lotion provided in Application Example 1 comprises the following steps:

[0082] The chemical sunscreen and emulsifier are mixed and heated to 85°C for homogenization, and then cooled to 45°C. A film former, an emollient, and a physical sunscreen are then added and homogenized into a uniform oil phase. A moisturizer, a thickener, and water are then added at 25°C for emulsification. After the emulsification is completed, a plant sunscreen synergistic composition and a preservative are added and stirred to obtain a sunscreen lotion.

[0083] The preparation methods of the sunscreen lotions provided in Application Examples 2-12, Comparative Application Examples 1-10 and Blank Application Examples are consistent with that in Application Example 1.

[0084] Effect Example 1

[0085] The present invention explores the performance of the plant sunscreen synergistic composition prepared in Examples 1-8 and Comparative Examples 1-10, including the following aspects:

[0086] 1. Ultraviolet absorption capacity

[0087] Take 1g of the sample and evenly apply it to a piece of 3M tape. Place it in a 37°C incubator for 20 minutes. Then remove it and attach it to the light-transmitting side of a quartz cuvette. Use a UV spectrophotometer to measure the UV absorbance in the 280-400nm range. UV absorbance values ​​are obtained every 10nm of UV wavelength. The UV absorbance value for a particular wavelength is the arithmetic average of the UV absorbance values ​​at each point within that wavelength. Record the UVB absorbance of the product. The data obtained are shown in Table 3.

[0088] 2. Anti-inflammatory effect

[0089] Plating: RAW264.7 cells in the logarithmic growth phase were seeded in 24-well plates at a cell density of 2*10 5 / mL, 0.5mL per well, mark the top cover of the cell plate after laying the plate, and place it in a carbon dioxide incubator for incubation for 24h;

[0090] Sample treatment: After incubating the cells for 24 h, the supernatant was removed and 0.5 mL of the following samples were added: ① blank control group: DMEM medium; ② LPS inflammation model group: LPS (1 μg / mL); ③ positive control group: 0.001% dexamethasone + LPS (1 μg / mL); ④ experimental group: 2% Example or Comparative Example + LPS (1 μg / mL);

[0091] Detection of inflammatory factors in cell supernatant: After the cells were treated with the sample for 24 hours, the supernatant was aspirated into a centrifuge tube with a pipette and centrifuged at 1000 rpm for 5 minutes. The precipitate was discarded and the supernatant was aspirated. The changes in the amount of inflammatory factors in the supernatant were detected using the Xinbosheng TNF-α Inflammatory Factor Detection Kit; the TNF-α inhibition rate was calculated as follows: TNF-α inhibition rate = (TNF-α amount in the model group - TNF-α amount in the experimental group) / TNF-α amount in the model group * 100%;

[0092] The obtained data are shown in Table 3.

[0093] Table 3

[0094]

[0095]

[0096] As can be seen from Table 3, the plant sunscreen synergistic composition provided by the present invention has good ultraviolet absorption and is also effective in anti-inflammatory. Specifically, the UVB absorbance of the obtained product is above 1.703, and the TNF-α inhibition rate is above 36.62%.

[0097] It can be seen from Examples 1-4 and Comparative Examples 1-2 that the mass of the components has a significant impact on the performance of the product. When the mass of the components of Comparative Examples 1-2 is not within the range given in the present invention, the overall performance of the obtained product is poor.

[0098] It can be seen from Example 1 and Comparative Examples 3-6 that the components in the plant sunscreen synergistic composition are indispensable. When any one of the components is missing, an excellent comprehensive effect cannot be achieved. It can be seen from Example 1 and Comparative Examples 7-10 that when other similar ingredients are used to replace the components in the present invention, the comprehensive performance of the obtained products also shows a significant downward trend.

[0099] Effect Example 2

[0100] The effects of the present invention are as follows: The performance of the sunscreen lotions prepared by application examples 1-12, comparative application examples 1-10 and blank application examples is explored, including the following aspects:

[0101] 1. SPF value test

[0102] The test was conducted in accordance with the requirements of the "Technical Specifications for Safety of Cosmetics" (2015 edition). 115 volunteers aged 32 to 60 were selected and divided into 23 groups, with 5 people in each group. The minimum erythema dose (MED) of the subjects' skin to ultraviolet radiation was predicted and the ultraviolet radiation dose was adjusted. A spot no less than 30 cm was selected on the back of the subject. 2 For normal skin area, (2.00±0.05)mg / cm 2 The test substance was evenly applied to the aforementioned area using a specific dosage. The irradiation dose was then selected according to the standard, with three irradiation scenarios: ① No test substance applied to the subject's skin; ② A control product (SPF 16.1±2.4, prepared according to the standard formula for high SPF standard P2 in the 2015 edition of the "Safety Technical Specifications for Cosmetics") was applied; and ③ The test substance was applied. After 24 hours, the experimental results were observed and the MED values ​​for each of the three scenarios were recorded.

[0103] SPF value calculation method: The SPF value of the test substance or blank control protecting a single subject is expressed as follows:

[0104] SPFi = MED value of protected skin / MED value of unprotected skin;

[0105] Calculate the arithmetic mean of the SPF values ​​of all subjects protected by the tested object, and round the result to one decimal place; the obtained data are shown in Table 4.

[0106] 2. Light stability test

[0107] The sample to be tested was placed under a solar simulator for 2 hours with a radiation intensity of 650W / m 2 , test the SPF value according to the above method. Calculate the SPF value retention rate of each sample based on the SPF value before and after irradiation according to the following formula to measure the photostability of the sunscreen product.

[0108] SPF value retention rate = (SPF 辐照后 / SPF 辐照前 )×100%.

[0109] The results are shown in Table 4.

[0110] 3. TEWL and a* value improvement test

[0111] Volunteers aged 18-45 years who had been exposed to a certain amount of ultraviolet light were selected. No skincare products were applied on the day of the visit. After cleansing their faces, the subjects sat quietly in a constant temperature and humidity room at 21±1°C and 50±10% for 30 minutes. Facial images were taken using Visia 7, and image analysis of the exported red zone images was performed to obtain the a* value. A transepidermal water loss probe was used to measure the skin's transepidermal water loss. The TM Hex (Courage + Khazaka) test measures initial facial TEWL. 115 subjects with initial facial a* values ​​above 15 were screened and divided into 23 groups of 5. Each group randomly applied the sunscreen product from the application example to their entire face while outdoors daily. TEWL testing and Visia 7 photography were performed on days 7 and 14. Improvements in TEWL and a* values ​​were analyzed at days 0, 7, and 14. Improvement was expressed as the improvement rate for each value, calculated as follows:

[0112] Improvement rate relative to the initial value = |(X 使用后 -X 使用前 ) / X 使用前 ×100%|; The results are shown in Table 4.

[0113] Table 4

[0114]

[0115]

[0116] As can be seen from Table 4, the sunscreen lotion provided by the present invention has high sun protection ability and sun protection durability, and also has excellent after-sun repair effect. Specifically, the SPF value of the obtained product is above 42.2, the SPF value retention rate after irradiation is above 85.9%, the TEWL improvement rate after 14 days of use is above 12.11%, and the a* value improvement rate is above 13.28%.

[0117] As can be seen from Application Examples 1-4 and Comparative Application Examples 1-2, the mass fraction of the components in the plant sunscreen synergistic composition has a significant impact on the performance of the product. When the mass fraction of the components in Comparative Application Examples 1-2 is not within the range given in the present invention, the overall effect of the obtained product is significantly reduced.

[0118] As can be seen from Application Example 1 and Comparative Application Examples 3-6, the components of the plant sunscreen synergistic composition are indispensable; as can be seen from Application Example 1 and Comparative Application Examples 7-10, when other similar ingredients are used to replace the components of the present invention, the comprehensive performance of the obtained products shows a significant downward trend;

[0119] As can be seen from Application Example 1 and Application Examples 5-8, when the preparation method of the components or the content of the active ingredients in the plant sunscreen synergistic composition is changed, the overall performance of the resulting product will also be significantly changed; as can be seen from Application Example 1 and Application Examples 11-13, when the type of physical sunscreen or chemical sunscreen in the sunscreen lotion is changed, the sunscreen ability and sunscreen durability of the resulting product will also change;

[0120] Among them, the pictures of volunteers who used the products in Application Example 1, Comparative Application Example 1 and Blank Application Example before and after use are as follows: Figure 1-3 As shown, from Figure 1-3 It can be seen that the product prepared by adopting the technical solution of the present invention has excellent effects of improving TEWL value and a* value.

[0121] Effect Example 3

[0122] The present invention explores the use experience scoring of the sunscreen lotion prepared in Application Example 1, specifically comprising the following steps:

[0123] Thirty Asian subjects with sensitive facial skin and recent sensitive skin symptoms were selected for the test. Sunscreen products were distributed to each subject at a rate of 2 mg / cm 2 After applying an appropriate amount of the product to the face, consumers were tested for sensitive skin for four weeks. They completed questionnaires assessing the degree of improvement in usage, discomfort, tolerance, mildness, sweat resistance, sun protection, and ease of washing. The results are shown in Table 5.

[0124] Table 5

[0125]

[0126] As can be seen from Table 5, the product provided by the present invention has a high consumer usage score, has a mild and non-irritating effect, and is suitable for people with sensitive skin.

[0127] Finally, it should be noted that the above embodiments are intended to illustrate the technical solutions of the present invention rather than to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents without departing from the essence and scope of the technical solutions of the present invention.

Claims

1. A plant sunscreen synergistic composition, characterized in that: The plant sunscreen synergistic composition comprises the following components in parts by weight: 0.1-1 parts of Cimicifuga extract, 0.3-1 parts of Eucommia ulmoides leaf extract, 1-3 parts of Rambutan peel extract, and 5-10 parts of Cyperus rotundus root extract; The preparation method of the Cimicifuga extract comprises the following steps: adding Cimicifuga to an ethanol aqueous solution for microwave extraction, filtering after the extraction is completed, collecting the filtrate, concentrating, and drying to obtain the Cimicifuga extract; In the preparation method of the Cimicifuga extract, the power of microwave extraction is 380-390W, and the time is 80-100s; The preparation method of the Cyperus rotundus root extract comprises the following steps: taking the Cyperus rotundus root, crushing it, adding it to an ethanol aqueous solution, heating and extracting it, filtering and concentrating it after the extraction is completed to obtain the Cyperus rotundus root extract; In the preparation method of the Cyperus rotundus root extract, the mass percentage of ethanol in the ethanol aqueous solution is 45-55%, and the mass volume ratio of the Cyperus rotundus root to the ethanol aqueous solution is 1 g: (3-7) mL.

2. The plant sunscreen synergistic composition according to claim 1, characterized in that The plant sunscreen synergistic composition comprises the following components in parts by weight: 0.5-0.6 parts of Cimicifuga extract, 0.4-0.5 parts of Eucommia ulmoides leaf extract, 1.5-2 parts of rambutan peel extract, and 7-8 parts of Cyperus rotundus root extract.

3. The plant sunscreen synergistic composition according to claim 1, characterized in that: In the plant sunscreen synergistic composition, the mass percentage of the rambutan peel extract is 11-22%.

4. The plant sunscreen synergistic composition according to claim 1, characterized in that The mass percentage of chlorogenic acid in the Eucommia ulmoides leaf extract is ≥5%.

5. The plant sunscreen synergistic composition according to claim 1, characterized in that: At least one of the following is met: a method for preparing the cimicifuga extract, the mass percentage of ethanol in the ethanol aqueous solution is 60-80%, the mass volume ratio of cimicifuga and ethanol aqueous solution is 1g: (15-25) mL; d. In the preparation method of the Cyperus rotundus root extract, the heating extraction temperature is 65-75°C and the time is 1-2h.

6. Use of the plant sunscreen synergistic composition according to any one of claims 1 to 5 in the preparation of sunscreen products.

7. A sunscreen lotion, characterized in that: The sunscreen lotion comprises the following components in percentage by mass: 10-15% of the plant sunscreen synergistic composition according to any one of claims 1 to 5, 5-7% of a physical sunscreen, 10-15% of a chemical sunscreen, 10-15% of an emollient, 2-3% of a film former, 5-12% of a moisturizer, 0.1-0.5% of a thickener, 0.5-2% of a preservative, 1-5% of an emulsifier, and the balance of water.

8. The sunscreen lotion according to claim 7, characterized in that The physical sunscreens include zinc oxide and titanium dioxide; the chemical sunscreens include diethylamino hydroxybenzoyl hexyl benzoate, ethylhexyl methoxycinnamate, bis-ethylhexyloxyphenol methoxyphenyl triazine, and ethylhexyl triazone.

9. The sunscreen lotion according to claim 7, characterized in that At least one of the following is met: e. The emollient comprises at least one of dicaprylyl carbonate and diisopropyl sebacate; f. The film-forming agent comprises at least one of trimethylsiloxysilicate and cyclopentasiloxane; g. The moisturizing agent comprises at least one of glycerol, 1,3-butylene glycol and 1,2-pentanediol; h. The thickener comprises at least one of xanthan gum, carbomer, and sodium polyacrylate; i. The preservative comprises at least one of p-hydroxyacetophenone and hexylene glycol; j. The emulsifier includes at least one of polyglyceryl-10 oleate, polysorbate 60, and polyglyceryl-3 diisostearate.

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