Tightening and wrinkle-fading composition containing retinol and application thereof in eye cream

Abstract

The invention relates to a wrinkle tightening and fading composition containing retinol and application of the wrinkle tightening and fading composition in eye cream. The wrinkle tightening and fading composition comprises a plant extract and retinol, the plant extract comprises a combination of a rice fermentation product, a rhodosporium aleurioides thallus extract, a haematococcus pluvialis extract and rapeseed oil. The invention creatively discovers that four plant extract components including the rice fermentation product, the rhodosporium aleurioides thallus extract, the haematococcus pluvialis extract and the rapeseed oil are combined with the retinol for use, the plant extract components can relieve the retinol in a multi-channel manner, and the formed composition has excellent moisturizing and wrinkle fading effects. In addition, the four plant extract components have a remarkable synergistic interaction effect. The tightening and wrinkle fading composition provided by the invention is very suitable for skin care products such as eye cream and the like, and is mild, non-irritant and good in stability.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics and relates to a retinol-containing composition for tightening and lightening wrinkles and application thereof in eye cream. Background Art

[0002] Skin aging is a natural process and the result of multiple endogenous and exogenous factors. It manifests itself in sagging, loose skin, wrinkles, loss of skin elasticity, dullness, spots, etc. The skin around the eyes is the thinnest part of the human body and is also the most active part. There are no oil glands and sweat glands around the eyes, and sebum that can moisturize the skin and prevent water evaporation cannot be secreted. In addition, with age, excessive ultraviolet radiation, and some bad eye habits, the epidermal tissue of the eye skin will become dry and thin, collagen and elastin will gradually be lost, and metabolism will slow down, making the skin prone to fine lines, crow's feet, dark circles, and eye bags due to dryness and roughness, and once the relevant lines appear, they are difficult to eliminate.

[0003] In order to delay the aging of the skin around the eyes, improve skin firmness and fade fine lines. In daily life, in addition to ensuring adequate sleep, paying attention to sun protection, and developing good living habits and eye habits, it is more important to do a good job of facial moisturizing and the selection and use of external skin care products. Retinol is one of the forms of vitamin A and has a highly effective anti-aging effect. On the one hand, retinol can increase the synthesis of collagen in the skin, and on the other hand, retinol can also induce the synthesis of dermal elastin and fibers, thereby effectively reducing fine lines and wrinkles and improving the firmness and elasticity of the skin. However, high concentrations of retinol are easy to irritate the skin, and too low a dosage cannot achieve the expected anti-aging effect. Therefore, it is of great significance to provide a skin care product that has a mild effect, soothing retinol, and can achieve efficient skin moisturizing, improve skin elasticity and firmness, and fade related lines. Summary of the invention

[0004] In view of the deficiencies of the prior art, the object of the present invention is to provide a retinol-containing composition for tightening and lightening wrinkles and its application in eye cream.

[0005] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0006] In a first aspect, the present invention provides a wrinkle-reducing and firming composition containing retinol, wherein the wrinkle-reducing and firming composition comprises a plant extract and retinol;

[0007] The plant extract includes a combination of rice fermentation product, Fanula ovata thallus extract, Haematococcus pluvialis extract and rapeseed oil.

[0008] In the present invention, plant extracts are used in combination with retinol components, and it is found that the plant extract components and the retinol components can cooperate synergistically to soothe retinol in multiple pathways, and the formed composition has excellent moisturizing and wrinkle-reducing effects.

[0009] In addition, rice fermentation product is a natural rice bran fermentation product, which can inhibit the activity of tyrosinase and promote the production of cholesterol. Cholesterol is an important component of sebum, which helps to improve the softness of the skin, and also has a certain activating effect on glutathione reductase, which can revitalize the skin and is suitable for anti-aging products; Fan algae fronds extract helps to enhance the activity of fibroblasts, and can promote the production of hyaluronic acid, increase keratin content, stimulate skin regeneration, improve skin elasticity and firmness, and have anti-wrinkle effects; Haematococcus pluvialis is a natural marine microalgae enriched with astaxanthin, which can avoid skin damage caused by ultraviolet rays, regulate skin pigmentation, scavenge free radicals, and has excellent antioxidant and anti-aging functions; rapeseed oil comes from rapeseed, contains saturated fatty acids and unsaturated fatty acids, has good lubricity and hydrophobicity, is easily absorbed by the skin, has no greasy feeling, can highly regulate the skin's protective barrier, and prevent the skin from external damage.

[0010] In the present invention, the above four specific plant extracts are used in combination, and it is found that they have significant synergistic effects in terms of moisturizing and tightening wrinkles.

[0011] Preferably, the mass ratio of the plant extract to retinol is (2-15):(1-7);

[0012] The above “2-15” may be, for example, 2, 3, 5, 8, 10, 11, 12, 13, 14, 15, etc.;

[0013] The above-mentioned "1-7" can be, for example, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6, 6.5, 7, etc. Other specific point values ​​within this numerical range can be selected, and they will not be elaborated here one by one.

[0014] Preferably, the plant extract comprises, by mass, 1-8 parts of rice fermentation product, 2-10 parts of Fanula ovata thallus extract, 0.04-2 parts of Haematococcus pluvialis extract and 0.05-1 part of rapeseed oil.

[0015] The above-mentioned “1-8 parts” can be, for example, 1 part, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, etc.;

[0016] The above-mentioned “2-10 parts” can be, for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, etc.;

[0017] The above-mentioned “0.04-2 parts” can be, for example, 0.04 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.5 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, etc.;

[0018] The above-mentioned "0.05-1 part" can be, for example, 0.05 part, 0.08 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc. Other specific point values ​​within the numerical range can be selected, and they will not be elaborated here one by one.

[0019] In the present invention, when the dosage ratio of each component in the plant extract is within the above specific range, it has a better effect of tightening and lightening wrinkles.

[0020] The frond body extract of Fandonia fasciata involved in the present invention is not limited to its specific preparation process. When it is prepared by the following process, its synergistic effect with the other three components in tightening and reducing wrinkles is more significant.

[0021] Preferably, the extract of the fronds of Fandonia solani is prepared by a preparation method comprising the following steps:

[0022] The fronds of P. pulex are mixed with a solution containing a low eutectic solvent for extraction, the extract is centrifuged to obtain the supernatant, concentrated and dried to obtain the P. pulex fronds extract.

[0023] In the present invention, a solution containing a low eutectic solvent is creatively used to extract the thallus of Fantasia pulex, which can effectively extract the active substances in the Fantasia pulex thallus. Compared with conventional hot water reflux extraction, a Fantasia pulex thallus extract with better effect can be obtained.

[0024] Preferably, the deep eutectic solvent is obtained by reacting a hydrogen bond acceptor and a hydrogen bond donor;

[0025] Preferably, the hydrogen bond donor is selected from any one or a combination of at least two of propylene glycol, glycerol, glycerol, glucose, glycolic acid, citric acid, L-ascorbic acid, polyethylene glycol, fructose or L-proline, preferably a combination of glucose and L-proline;

[0026] Preferably, the hydrogen bond acceptor is selected from any one or a combination of at least two of betaine, choline chloride or acetamide, preferably betaine and / or choline chloride.

[0027] Preferably, the molar ratio of the hydrogen bond acceptor to the hydrogen bond donor is (2-8):(1-3), for example, it can be 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, 2:3, etc. Other specific point values ​​within this numerical range can be selected and will not be described one by one here.

[0028] Preferably, the solution containing the low eutectic solvent also includes water, and the mass ratio of the low eutectic solvent to water in the mixed solvent is 10:1-1:1, for example, it can be 10:1, 9:1, 8:1, 7:1, 6:1, 5:1, 4:1, 3:1, 2:1, 1:1, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0029] Preferably, the solid-liquid ratio of the fronds of the fan algae to the solution containing the low eutectic solvent is 1:(5-20) g / mL, for example, it can be 1:5 g / mL, 1:6 g / mL, 1:8 g / mL, 1:10 g / mL, 1:12 g / mL, 1:15 g / mL, 1:16 g / mL, 1:18 g / mL, 1:20 g / mL, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0030] Preferably, the extraction temperature is 30-90°C, for example, it can be 30°C, 35°C, 40°C, 45°C, 50°C, 55°C, 60°C, 65°C, 70°C, 75°C, 80°C, 85°C, 90°C, etc.; the time is 10-60min, for example, it can be 10min, 15min, 20min, 25min, 30min, 35min, 40min, 45min, 50min, 55min, 60min, etc., and other specific point values ​​within the numerical range can be selected, which will not be described one by one here.

[0031] The rice fermentation product and rapeseed oil component involved in the present invention are not limited in their specific preparation process and source, and products obtained by conventional extraction and preparation processes and regular commercially available products can be used in the scheme of the present invention.

[0032] The Haematococcus pluvialis extract involved in the present invention is not limited to its specific preparation process. When it is prepared by the following process, its synergistic effect with the other three components in tightening and lightening wrinkles is more significant.

[0033] Preferably, the Haematococcus pluvialis extract is prepared by a preparation method comprising the following steps:

[0034] The Haematococcus pluvialis is crushed and sieved, mixed with a complex enzyme solution, and subjected to microfluidization extraction. The extract is centrifuged to obtain the supernatant, concentrated and dried to obtain the Haematococcus pluvialis extract.

[0035] The enzymes in the complex enzyme include cellulase and protease.

[0036] Preferably, the addition amount of the complex enzyme in the complex enzyme solution is 0.2‰-0.6‰ of the mass of Haematococcus pluvialis, for example, it can be 0.2‰, 0.25‰, 0.3‰, 0.35‰, 0.4‰, 0.45‰, 0.5‰, 0.55‰, 0.6‰, etc. Other specific point values ​​within this numerical range can be selected, and they will not be described one by one here.

[0037] Preferably, the ratio of the Haematococcus pluvialis to the complex enzyme solution is 1:(10-50) g / mL, for example, it can be 1:10 g / mL, 1:15 g / mL, 1:20 g / mL, 1:25 g / mL, 1:30 g / mL, 1:35 g / mL, 1:40 g / mL, 1:45 g / mL, 1:50 g / mL, etc. Other specific point values ​​within this numerical range can be selected, and they will not be repeated here one by one.

[0038] Preferably, the pressure of the microfluidic extraction is 20-300 MPa, for example, it can be 20 MPa, 50 MPa, 75 MPa, 100 MPa, 125 MPa, 150 MPa, 180 MPa, 200 MPa, 240 MPa, 260 MPa, 280 MPa, 300 MPa, etc.; the temperature is 20-50°C, for example, it can be 20°C, 22°C, 25°C, 28°C, 30°C, 32°C, 35°C, 38°C, 40°C, 42°C, 45°C, 48°C, 50°C, etc.; the number of material cycles is 2-10 times, for example, it can be 2 times, 3 times, 4 times, 5 times, 6 times, 7 times, 8 times, 9 times, 10 times.

[0039] In the present invention, the crushed Haematococcus pluvialis is extracted by enzymatic hydrolysis, and the microfluidization process is used to assist the extraction, which can further improve the extraction effect. Compared with the conventional static enzymatic hydrolysis process, it has a better extraction effect and can obtain a Haematococcus pluvialis extract with better efficacy.

[0040] In a second aspect, the present invention provides a use of the wrinkle-reducing and firming composition according to the first aspect in cosmetics;

[0041] The cosmetics include lotions, creams, skin care water, skin care essences or facial masks.

[0042] In a second aspect, the present invention provides a retinol eye cream with the effect of firming and reducing wrinkles, wherein the retinol eye cream comprises 1-10 parts by mass of the firming and reducing wrinkles composition described in the first aspect, for example, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10 parts, etc.

[0043] Preferably, the firming and wrinkle-lightening eye cream further comprises, by weight, any one of 4-20 parts of moisturizer, 2-12 parts of emulsifier, 2-15 parts of emollient, 0.05-2 parts of thickener, 0.1-2 parts of skin conditioner, 0.1-2 parts of antioxidant, 0.001-0.5 parts of chelating agent, 0.001-0.1 parts of fragrance or 40-70 parts of water, or a combination of at least two of them.

[0044] The above “4-20 parts” may be, for example, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 10 parts, 12 parts, 15 parts, 18 parts, 20 parts, etc.;

[0045] The above-mentioned “2-12 parts” can be, for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, etc.;

[0046] The above-mentioned “2-15 parts” can be, for example, 2 parts, 3 parts, 4 parts, 5 parts, 6 parts, 7 parts, 8 parts, 9 parts, 10 parts, 11 parts, 12 parts, 13 parts, 14 parts, 15 parts, etc.;

[0047] The above-mentioned “0.05-2 parts” can be, for example, 0.05 parts, 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, etc.;

[0048] The above-mentioned “0.1-2 parts” can be, for example, 0.1 parts, 0.2 parts, 0.5 parts, 0.8 parts, 1 parts, 1.2 parts, 1.5 parts, 1.8 parts, 2 parts, etc.;

[0049] The above-mentioned “0.001-0.5 parts” can be, for example, 0.001 parts, 0.005 parts, 0.01 parts, 0.02 parts, 0.05 parts, 0.08 parts, 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, etc.;

[0050] The above-mentioned “0.001-0.1 part” can be, for example, 0.001 part, 0.005 part, 0.01 part, 0.02 part, 0.03 part, 0.04 part, 0.05 part, 0.06 part, 0.07 part, 0.08 part, 0.09 part, 0.1 part, etc.;

[0051] The above-mentioned "40-70 parts" can be, for example, 40 parts, 42 parts, 45 parts, 48 ​​parts, 50 parts, 52 parts, 55 parts, 58 parts, 60 parts, 62 parts, 65 parts, 68 parts, 70 parts, etc. Other specific point values ​​within the numerical range can be selected, and they will not be elaborated here one by one.

[0052] Preferably, the moisturizing agent includes any one of isononyl isononanoate, sodium hyaluronate, allantoin, ammonium acryloyldimethyltaurate / VP copolymer, ethylhexylglycerin, glyceryl glucoside, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, magnesium chloride, caprylic / capric triglyceride, polydimethylsiloxane, glycerin, β-glucan, butylene glycol, 1,2-pentanediol, 1,2-hexanediol or caprylyl glycol, or a combination of at least two thereof;

[0053] Preferably, the emulsifier includes any one of glyceryl stearate, sodium stearoyl lactylate, polysorbate-60, lecithin, poloxamer 407, polyglyceryl-10 oleate, polyglyceryl-10 stearate, cetearyl alcohol, behenyl alcohol, sodium acrylate / sodium acryloyldimethyl taurate copolymer, sorbitan isostearate or sorbitan oleate, or a combination of at least two thereof.

[0054] Preferably, the emollient includes any one of cetearyl dimethicone crosspolymer, butylene glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), myristyl myristate, isohexadecane or polysorbate-80, or a combination of at least two thereof.

[0055] Preferably, the thickener comprises any one of C30-45 alkyl dimethicone, xanthan gum, carbomer or ethyl cellulose, or a combination of at least two thereof.

[0056] Preferably, the skin conditioning agent comprises any one of saffron flower extract, sophora flavescens root extract, tribulus terrestris fruit extract or cornus officinalis fruit extract, or a combination of at least two thereof.

[0057] Preferably, the antioxidant comprises any one of ubiquinone, astaxanthin or tocopherol acetate, or a combination of at least two thereof.

[0058] Preferably, the chelating agent comprises caprylhydroxamic acid and / or disodium EDTA.

[0059] Compared with the prior art, the present invention has the following beneficial effects:

[0060] In the present invention, plant extract components are used in combination with retinol, and it is found that the plant extract can soothe retinol in multiple ways, and the formed composition has excellent moisturizing, firming and wrinkle-reducing effects. In addition, four specific plant extracts, namely, rice fermentation products, Fan algae thallus extracts, Haematococcus pluvialis extracts and rapeseed oil, are used. It is found that these four components can interact with each other and positively influence each other, and have a synergistic promotion effect in terms of moisturizing and firming and wrinkle-reducing effects that is difficult for technicians in this field to predict, and can further enhance the moisturizing and anti-wrinkle effects of the product. DETAILED DESCRIPTION

[0061] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0062] The rice fermentation products mentioned below are CelCaper sake lees extract Pro products produced by Guangdong MaruMei Biotechnology Co., Ltd. The main active ingredients in the rice fermentation products are natural fruit acids, enzymes, amino acids, sugars, proteins, polyphenols and flavonoids;

[0063] Sodium hyaluronate comes from Hybloom of Freda TM product;

[0064] Tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate comes from Guangdong Runpei Chemical Co., Ltd. product;

[0065] Crocus flower extract comes from SENS'FLOWER SD-SC product of Youran Company, and the main active substances are carotenoids, flavonoids, anthocyanins, terpenoids and phenolic acid;

[0066] The mixed extract (sobacteria root extract + tribulus terrestris fruit extract + cornus officinalis fruit extract) comes from the Sensysoothe product of Guangzhou Miaosen Biotechnology Co., Ltd. The active ingredients include: sophora root extract, the key active ingredient is oxymatrine; tribulus terrestris fruit extract, the key active ingredient is tribulus saponin; cornus officinalis fruit extract, the key active ingredient is ursolic acid;

[0067] Retinol comes from the Retinol Liposomal (10%) product of Youran Company;

[0068] Rapeseed oil comes from Zhongran Company's Astabio AW1 product, and its main active ingredients are saturated fatty acids and unsaturated fatty acids;

[0069] Fragrance-1 comes from PINK CALYPSO 86347247 of Biohobo Company;

[0070] Fragrance-2 comes from VERVEINE 1423402 product of Youran Company.

[0071] The weight percentages of the rice fermentation product, sodium hyaluronate, tetradecylaminobutyryl valinamidobutyric acid urea trifluoroacetate, saffron flower extract, mixed extract, retinol and rapeseed oil involved below are all calculated based on the actual content of effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, moisturizers, thickeners, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0072] Preparation Example 1

[0073] This preparation example provides a thallus extract of P. fasciata, which is prepared by the following method:

[0074] (1) After freeze-drying and grinding the thallus tissue of the fan algae, the thallus tissue was mixed with a solution containing a low eutectic solvent [(glucose + L-proline): betaine = 5:1, glucose: L-proline = 1:1, molar ratio] for leaching, wherein the mass ratio of the low eutectic solvent to water in the solution was 5:1, and the solid-liquid ratio of the fan algae thallus to the solution containing the low eutectic solvent was 1:10 g / mL, and the leaching was carried out under the condition of heating in a water bath at a temperature of 60° C. for 30 min;

[0075] (2) After the extract is centrifuged, the supernatant is collected, the precipitate is mixed with water, the extraction process in step (1) and the centrifugation step in step (2) are repeated once, the supernatant is collected and combined, and freeze-dried to obtain the frond extract of Fan-shaped algae.

[0076] This preparation example also provides a Haematococcus pluvialis extract, which is prepared by the following method:

[0077] The Haematococcus pluvialis was freeze-dried and crushed, mixed with a composite enzyme solution (composed of cellulase and protease in a mass ratio of 1:1) and then subjected to microfluidization extraction treatment. The amount of composite enzyme added to the composite enzyme solution was 0.2‰ of the mass of the Haematococcus pluvialis, the material-liquid ratio of the Haematococcus pluvialis to the composite enzyme solution was 1:20 g / mL, and the conditions for microfluidization extraction were: a pressure of 75 MPa, a temperature of 40°C, and a material-liquid circulation number of 5 times; the treated liquid was centrifuged to obtain the supernatant, which was concentrated and dried to obtain the Haematococcus pluvialis extract.

[0078] Preparation Example 2

[0079] This preparation example provides a thallus extract of P. fasciata, which is prepared by the following method:

[0080] (1) freeze-dried and ground the thallus tissue of the fan algae, and then mixed with a solution containing a low eutectic solvent [(fructose + citric acid): choline chloride = 7:1, fructose: citric acid = 3:2, molar ratio] for leaching, wherein the mass ratio of the low eutectic solvent to water in the solution is 3:1, and the solid-liquid ratio of the fan algae thallus to the solution containing the low eutectic solvent is 1:6 g / mL, and the leaching is carried out under the condition of heating in a water bath at a temperature of 35° C. for 50 min;

[0081] (2) After the extract is centrifuged, the supernatant is collected, the precipitate is mixed with water, the extraction process in step (1) and the centrifugation step in step (2) are repeated once, the supernatant is collected and combined, and freeze-dried to obtain the frond extract of Fan-shaped algae.

[0082] This preparation example also provides a Haematococcus pluvialis extract, which is prepared by the following method:

[0083] The Haematococcus pluvialis was freeze-dried and crushed, mixed with a composite enzyme solution (composed of cellulase and protease in a mass ratio of 3:1) and then subjected to microfluidization extraction treatment. The amount of composite enzyme added to the composite enzyme solution was 0.35‰ of the mass of the Haematococcus pluvialis, the material-liquid ratio of the Haematococcus pluvialis to the composite enzyme solution was 1:15 g / mL, and the conditions for microfluidic extraction were: a pressure of 220 MPa, a temperature of 20°C, and a material-liquid circulation number of 3 times; the treated liquid was centrifuged to obtain the supernatant, which was concentrated and dried to obtain the Haematococcus pluvialis extract.

[0084] Preparation Example 3

[0085] This preparation example provides a thallus extract of P. fasciata, which is prepared by the following method:

[0086] (1) freeze-dried and ground the thallus tissue of the fan algae, and then mixed with a solution containing a low eutectic solvent [(glycerol + glycerol): choline chloride = 3:1, glycerol: glycerol = 4:1, molar ratio] for leaching, wherein the mass ratio of the low eutectic solvent to water in the solution is 8:1, and the solid-liquid ratio of the fan algae thallus to the solution containing the low eutectic solvent is 1:18 g / mL, and the leaching is carried out under the condition of water bath heating at a temperature of 80° C. for 20 min;

[0087] (2) After the extract is centrifuged, the supernatant is collected, the precipitate is mixed with water, the extraction process in step (1) and the centrifugation step in step (2) are repeated once, the supernatant is collected and combined, and freeze-dried to obtain the frond extract of Fan-shaped algae.

[0088] This preparation example also provides a Haematococcus pluvialis extract, which is prepared by the following method:

[0089] The Haematococcus pluvialis was freeze-dried and crushed, mixed with a composite enzyme solution (composed of cellulase and protease in a mass ratio of 1:5) and then subjected to microfluidization extraction treatment. The amount of composite enzyme added to the composite enzyme solution was 0.5‰ of the mass of the Haematococcus pluvialis, the material-liquid ratio of the Haematococcus pluvialis to the composite enzyme solution was 1:40 g / mL, and the conditions for microfluidic extraction were: a pressure of 30 MPa, a temperature of 50°C, and a material-liquid circulation number of 8 times; the treated liquid was centrifuged to obtain the supernatant, which was concentrated and dried to obtain the Haematococcus pluvialis extract.

[0090] Preparation Example 4

[0091] The present invention provides a frond extract of Fandonia floribunda, which is prepared by the following method:

[0092] The thallus tissue of Fan-shaped algae was freeze-dried and ground, mixed with water at a solid-liquid ratio of 1g:15mL, heated under reflux for extraction for 3h, centrifuged to obtain the supernatant, and the precipitate was added with water to repeat the above extraction and centrifugation process once. The two supernatants were combined, concentrated and freeze-dried to obtain the Fan-shaped algae thallus extract.

[0093] Preparation Example 5

[0094] The present invention provides a Haematococcus pluvialis extract, which is prepared by the following method:

[0095] The freeze-dried and crushed Haematococcus pluvialis and the complex enzyme solution in Preparation Example 1 are used. The preparation method thereof is different from that in Preparation Example 1 only in that the extraction in step (1) is performed by heating in a water bath at 40° C. and standing for enzymatic hydrolysis for 2 h, the extract is centrifuged to obtain the supernatant, and the supernatant is concentrated and dried to obtain the Haematococcus pluvialis extract.

[0096] Example 1

[0097] The present embodiment provides a firming and wrinkle-reducing composition, which is composed, by mass, of 2 parts of retinol, 5 parts of rice fermentation product, 4.5 parts of Fanula umbellata thallus extract (Preparation Example 1), 1 part of Haematococcus pluvialis extract (Preparation Example 1) and 0.5 parts of rapeseed oil.

[0098] Example 2

[0099] The present embodiment provides a firming and wrinkle-reducing composition, which is composed, by mass, of 1 part of retinol, 7 parts of rice fermentation product, 2 parts of Fanula umbellata thallus extract (Preparation Example 2), 1.8 parts of Haematococcus pluvialis extract (Preparation Example 2) and 0.2 parts of rapeseed oil.

[0100] Example 3

[0101] The present embodiment provides a firming and wrinkle-reducing composition, which is composed, by mass, of 1.5 parts of retinol, 1 part of rice fermentation product, 9 parts of Fanula umbellata thallus extract (Preparation Example 3), 0.1 part of Haematococcus pluvialis extract (Preparation Example 3) and 0.9 part of rapeseed oil.

[0102] Example 4

[0103] This embodiment provides a wrinkle-tightening and wrinkle-reducing composition, which differs from Embodiment 1 only in that the frond body extract of P. truncatula (Preparation Example 1) is replaced with an equal amount of the frond body extract of P. truncatula (Preparation Example 4).

[0104] Example 5

[0105] This embodiment provides a wrinkle-tightening and wrinkle-reducing composition, which differs from Embodiment 1 only in that the Haematococcus pluvialis extract (Preparation Example 1) is replaced with an equal amount of Haematococcus pluvialis extract (Preparation Example 5).

[0106] Comparative Example 1

[0107] This comparative example provides a wrinkle-reducing and firming composition, which differs from Example 1 only in that it does not contain rice fermentation product, and its reduced amount is proportionally distributed among the three components of Fanzolla thallus extract, Haematococcus pluvialis extract and rapeseed oil.

[0108] Comparative Example 2

[0109] This comparative example provides a wrinkle-reducing and firming composition, which differs from Example 1 only in that it does not contain the frond extract of Fanula ovata, and its reduced amount is proportionally distributed among the three components of rice fermentation product, Haematococcus pluvialis extract and rapeseed oil.

[0110] Comparative Example 3

[0111] This comparative example provides a wrinkle-reducing and firming composition, which differs from Example 1 only in that it does not contain the Haematococcus pluvialis extract, and the reduced amount of the extract is proportionally distributed among the three components of rice fermentation product, Fan algae thallus extract and rapeseed oil.

[0112] Comparative Example 4

[0113] This comparative example provides a wrinkle-reducing and firming composition, which differs from Example 1 only in that it does not contain rapeseed oil, and the reduced amount of rapeseed oil is proportionally distributed among the three components of rice fermentation product, Fan algae thallus extract and Haematococcus pluvialis extract.

[0114] Comparative Example 5

[0115] This comparative example provides a wrinkle-reducing and firming composition, which differs from Example 1 only in that it does not contain retinol, and its reduced amount is proportionally distributed to four plant extract components: rice fermentation product, Fan algae thallus extract, Haematococcus pluvialis extract and rapeseed oil.

[0116] Application Example 1

[0117] This application example provides a retinol eye cream with the effect of tightening and lightening wrinkles, and its formula is as follows:

[0118]

[0119]

[0120] Its preparation method is:

[0121] (1) Add the first moisturizer, the first emulsifier, the first emollient, the thickener, and the antioxidant (ubiquinone + tocopherol acetate) into the oil phase pot, stir and heat to 85° C., and keep warm for 10 minutes.

[0122] (2) Add the second moisturizer, the wrinkle-reducing composition (rice fermentation product) and part of the water into the water phase pot, stir and heat to 80° C., and keep warm for 10 minutes.

[0123] (3) After the insulation is completed, the mixture in the water phase pot and the oil phase pot is pumped into the emulsifier pot, homogenized at 2500 rpm for 8 minutes, and then the second emulsifier and the second emollient are added. The mixture is homogenized at 2000 rpm for 8 minutes, and the temperature is lowered to 45° C. The remaining raw material components are added, and the mixture is stirred at 500 rpm for 10 minutes to prepare the eye cream product.

[0124] Application Example 2-5

[0125] This application example provides four retinol eye creams with the effect of tightening and reducing wrinkles. The difference between the formula and the application example is that the composition prepared in Example 1 is replaced with an equal amount of the composition prepared in Examples 2-5, and the other ingredients and contents remain unchanged. The preparation method refers to Application Example 1.

[0126] Comparative Application Examples 1-5

[0127] This comparative application example provides five types of firming and wrinkle-lightening eye creams, the formulas of which differ from those of the application examples only in that the composition prepared in Example 1 is replaced with equal amounts of the compositions prepared in Comparative Examples 1-5, respectively, and the other ingredients and contents remain unchanged. The preparation method refers to that in Application Example 1.

[0128] Test Example 1

[0129] Matrix Efficacy Test Screening

[0130] (1) A total of 100 healthy Chinese women with dry, rough, loose, inelastic faces and obvious wrinkles around the eyes and mouth were selected. The age range was 35-60 years old, with an average age of 45±5.5 years old. The subjects were divided into 10 groups, with 10 people in each group. After arriving at the laboratory, the subjects cleaned their faces with facial cleanser, sat quietly for 30 minutes, performed a half-face test on the skin, applied the product and massaged it until it was absorbed for 15 minutes, and then tested the skin again. The changes before and after the skin were statistically analyzed to obtain the change rate.

[0131] (2) Test items include:

[0132] The moisture content of the stratum corneum of the skin was tested using the Corneometer CM 825 (Courage & Khazaka, Germany);

[0133] Skin elasticity and firmness were tested using the Cutometer MPA580 (Courage & Khazaka, Germany).

[0134] (4) The wrinkle-reducing and firming compositions of Examples 1-5 and Comparative Examples 1-5 were dissolved in pure water respectively, so that the concentration of the compositions in each group of samples was 5%, and tested. The test environment was at a temperature of 21° C. and a humidity of 48.5%, which met the requirements of the scheme design. The improvement after using the product (%) = (data after use - data before use) / data before use × 100%. The results are shown in Table 1:

[0135] Table 1

[0136] Improvement rate of stratum corneum moisture content Skin elasticity R5 value improvement rate Skin firmness R0 improvement rate Example 1 41.34％ 27.67％ 7.78％ Example 2 40.98％ 27.21％ 7.71％ Example 3 41.03％ 27.03％ 7.26％ Example 4 39.77％ 26.99％ 6.59％ Example 5 39.65％ 26.83％ 6.98％ Comparative Example 1 35.27％ 25.03％ 5.42％ Comparative Example 2 38.26％ 24.88％ 5.33％ Comparative Example 3 37.65％ 23.02％ 4.98％ Comparative Example 4 35.99％ 23.97％ 4.77％ Comparative Example 5 37.01％ 23.65％ 4.03％

[0137] From the data in the table we can see that:

[0138] (1) It can be seen from Examples 1 to 3 that the wrinkle-reducing and firming composition provided by the present invention has significant moisturizing and firming effects, among which Example 1 has the best effect.

[0139] (2) By comparing Example 1 with Example 4, it can be seen that the use of a solution containing a low eutectic solvent to extract the thallus of Fan algae can effectively extract the active substances in the Fan algae thallus, and compared with conventional hot water reflux extraction, a Fan algae thallus extract with better effect can be obtained.

[0140] (3) By comparing Example 1 with Example 5, it can be seen that the present invention adopts enzymatic hydrolysis to extract the crushed Haematococcus pluvialis, and uses microfluidization technology to assist extraction, which can further improve the extraction effect. Compared with the conventional static enzymatic hydrolysis process, it has a better extraction effect and can obtain a Haematococcus pluvialis extract with better efficacy.

[0141] (4) By comparing Example 1 with Comparative Examples 1-4, it can be seen that when Comparative Examples 1-4 do not contain any of the four components of rice fermentation product, Fan algae thallus extract, Haematococcus pluvialis extract and rapeseed oil, the prepared compositions are less effective in moisturizing and improving skin elasticity than those in Example 1. It can be seen that the four components of rice fermentation product, Fan algae thallus extract, Haematococcus pluvialis extract and rapeseed oil used in the present invention can cooperate with each other, synergistically enhance the effect, and improve the skin condition.

[0142] (5) By comparing Example 1 with Comparative Example 5, it can be seen that the present invention uses a plant extract component and a retinol component in combination, and it is found that the plant extract component can relieve retinol in multiple ways, and the formed composition has excellent moisturizing and wrinkle-reducing effects. In addition, it can overcome the problem that low-concentration retinol has poor effect and high-concentration dosage is easy to irritate the skin.

[0143] Test Example 2

[0144] Eye cream efficacy test

[0145] (1) Chinese healthy women with dry, rough, loose, inelastic skin and obvious wrinkles around the eyes and mouth were selected. The subjects had no allergic diseases, no history of allergies to cosmetics and other topical preparations, and were used to using basic moisturizing lotions and lotions / creams on a daily basis. A total of 100 subjects were included, with an age range of 35-60 years old and an average age of 45±5.5 years old. The subjects were divided into 10 groups, with 10 people in each group.

[0146] (2) After cleansing and using toner every night, the subjects took an appropriate amount of eye cream and evenly applied it around the eyes. Gently apply it to the skin around the eyes and the nasolabial folds. Use the gilded crystal massage head to gently massage the eye area from the inside to the outside and from the bottom to the top until it is absorbed. Use it once a day for 28 consecutive days. Statistical analysis of the changes before and after the skin was performed to obtain the change rate.

[0147] (3) Test items include:

[0148] Corneometer CM 825 is a skin stratum corneum moisture tester that tests the moisture content of the skin stratum corneum.

[0149] Cutometer MPA580 is a skin elasticity tester that tests skin elasticity and firmness.

[0150] The skin fast optical imaging system Evaskin-S5 tests the improvement effect of various lines.

[0151] (3) The test product is the eye cream product prepared in Application Examples 1-5 and Comparative Application Examples 1-5. The test environment is at a temperature of 20.2° C. to 21.8° C. and a humidity of 42.4% to 53.9%, which meets the requirements of the scheme design. The improvement after using the product (%) = (data after use - data before use) / data before use × 100%. The results are shown in Table 2:

[0152] Table 2

[0153]

[0154] From the data in the table, it can be seen that the present invention uses plant extracts in combination with retinol components, and it is found that the plant extract components can relieve retinol in multiple ways, and the formed composition has excellent moisturizing and wrinkle-lightening effects. In addition, the four components of rice fermentation product, Fan algae frond extract, Haematococcus pluvialis extract and rapeseed oil have a certain synergistic effect in moisturizing, anti-wrinkle and improving skin elasticity.

[0155] Test Example 3

[0156] Product safety testing

[0157] According to the inclusion criteria, 25 women and 25 men were selected to participate in the test and randomly divided into 5 groups (5 men and 5 women in each group) to receive the trial. Take an appropriate amount of sample and place it in the chamber of the spot test tape. The spot tester with the sample is applied to the skin of the curved side of the forearm of the subject with medical tape for 24 hours. 30 minutes after removing the test spot tester, observe the skin reaction after the indentation disappears. For those with negative results, observe again 24 hours and 48 hours after the test, and record the skin reaction results. The reaction degree grading standard is as shown in the following table.

[0158]

[0159] The results show that the products prepared in Application Examples 1-5 showed negative reactions 24h and 48h after the test, and there was no irritation or erythema. It can be seen that the eye cream product prepared by the present invention has no irritation to the skin and no positive reaction, which proves that the product is highly safe, less irritating, and milder.

[0160] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0161] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0162] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A retinol-containing composition for tightening and lightening wrinkles, characterized in that: The wrinkle-reducing and firming composition comprises plant extracts and retinol; The plant extract includes a combination of rice fermentation product, Fanula ovata thallus extract, Haematococcus pluvialis extract and rapeseed oil.

2. The wrinkle-reducing and firming composition according to claim 1, characterized in that: The mass ratio of the plant extract to retinol is (2-15):(1-7).

3. The wrinkle-reducing and firming composition according to claim 1, characterized in that: The plant extract comprises, by weight, 1-8 parts of rice fermentation product, 2-10 parts of Fanula ovata thallus extract, 0.04-2 parts of Haematococcus pluvialis extract and 0.05-1 part of rapeseed oil.

4. The wrinkle-reducing and firming composition according to claim 1 or 3, characterized in that: The frond extract of Fandonia glomerata is prepared by a preparation method comprising the following steps: The fronds of P. pulex are mixed with a solution containing a low eutectic solvent for extraction, the extract is centrifuged to obtain the supernatant, concentrated and dried to obtain the P. pulex fronds extract.

5. The wrinkle-reducing and firming composition according to claim 4, characterized in that: The deep eutectic solvent is obtained by reacting a hydrogen bond acceptor and a hydrogen bond donor; Preferably, the hydrogen bond donor is selected from any one or a combination of at least two of propylene glycol, glycerol, glycerol, glucose, glycolic acid, citric acid, L-ascorbic acid, polyethylene glycol, fructose or L-proline; Preferably, the hydrogen bond acceptor is selected from any one of betaine, choline chloride or acetamide, or a combination of at least two thereof.

6. The wrinkle-reducing and firming composition according to claim 1 or 3, characterized in that: The Haematococcus pluvialis extract is prepared by a preparation method comprising the following steps: The Haematococcus pluvialis is crushed and sieved, mixed with a complex enzyme solution, and subjected to microfluidization extraction. The extract is centrifuged to obtain the supernatant, concentrated and dried to obtain the Haematococcus pluvialis extract. The enzymes in the complex enzyme include cellulase and protease.

7. Use of the wrinkle-reducing composition according to any one of claims 1 to 6 in cosmetics; The cosmetics include lotions, creams, skin care water, skin care essences or facial masks.

8. A retinol eye cream with the effect of tightening and lightening wrinkles, characterized in that: The retinol eye cream comprises 1-10 parts by mass of the wrinkle-reducing composition according to any one of claims 1-6.

9. The retinol eye cream according to claim 8, characterized in that The firming and wrinkle-lightening eye cream also includes, by weight, any one of 4-20 parts of moisturizer, 2-12 parts of emulsifier, 2-15 parts of emollient, 0.05-2 parts of thickener, 0.1-2 parts of skin conditioner, 0.1-2 parts of antioxidant, 0.001-0.5 parts of chelating agent, 0.001-0.1 parts of fragrance or 40-70 parts of water, or a combination of at least two of them.

10. The retinol eye cream according to claim 9, characterized in that: The moisturizing agent includes any one of isononyl isononanoate, sodium hyaluronate, allantoin, ammonium acryloyldimethyltaurate / VP copolymer, ethylhexylglycerin, glyceryl glucoside, tetradecylaminobutyrylvalinamidobutyric acid urea trifluoroacetate, magnesium chloride, caprylic / capric triglyceride, polydimethylsiloxane, glycerin, β-glucan, butylene glycol, 1,2-pentanediol, 1,2-hexanediol or caprylyl glycol, or a combination of at least two thereof; Preferably, the emulsifier includes any one or a combination of at least two of glyceryl stearate, sodium stearoyl lactylate, polysorbate-60, lecithin, poloxamer 407, polyglyceryl-10 oleate, polyglyceryl-10 stearate, cetearyl alcohol, behenyl alcohol, sodium acrylate / sodium acryloyldimethyl taurate copolymer, sorbitan isostearate or sorbitan oleate; Preferably, the emollient includes any one or a combination of at least two of cetearyl dimethicone crosspolymer, butylene glycol dicaprylate / dicaprate, pentaerythritol tetra(ethylhexanoate), myristyl myristate, isohexadecane or polysorbate-80; Preferably, the thickener comprises any one or a combination of at least two of C30-45 alkyl dimethicone, xanthan gum, carbomer or ethyl cellulose; Preferably, the skin conditioning agent comprises any one or a combination of at least two of Crocus sativus flower extract, Sophora flavescens root extract, Tribulus terrestris fruit extract or Cornus officinalis fruit extract; Preferably, the antioxidant comprises any one or a combination of at least two of ubiquinone, astaxanthin or tocopherol acetate; Preferably, the chelating agent comprises caprylhydroxamic acid and / or disodium EDTA.

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