Skin repairing gel based on aloe extracting solution and preparation method thereof

Abstract

The invention relates to the technical field of skin repairing and nursing, in particular to skin repairing gel based on an aloe extracting solution and a preparation method of the skin repairing gel. The aloe repair mask is prepared from the following components in parts by weight: 2 to 8 parts of repair agent, 33 to 45 parts of aloe extracting solution, 0.5 to 2 parts of hydroxypropyl methyl cellulose, 6 to 9 parts of glycerinum, 4 to 8 parts of sodium hyaluronate, 0.1 to 0.3 part of phenoxyethanol, 0.2 to 0.4 part of triethanolamine and 0.1 to 0.5 part of butylated hydroxyanisole, wherein the repairing agent is prepared by mixing curdione and modified polyethylene glycol-polycaprolactone according to the mass ratio of 1: (5-10); the modified polyethylene glycol-polycaprolactone is obtained by modifying polyethylene glycol-polycaprolactone and 4-aminocinnamic acid in a mass ratio of 1: (0.05-0.07) under the condition that N-(3-dimethylaminopropyl)-N '-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine are mixed. By adopting the repairing agent disclosed by the invention, not only is the antibacterial property of the gel improved, but also the adhesion of the gel on skin tissues is enhanced.

Description

Technical Field

[0001] The invention relates to the technical field of skin repair and care, in particular to a skin repair gel based on aloe extract and a preparation method thereof. Background Art

[0002] Aloe vera, a magical species known as the "plant doctor" since ancient times, has rich nutrients and active substances in its leaves, making it an ideal choice for making skin repair gel. Aloe vera extract is rich in polysaccharides, amino acids, vitamins, minerals and a variety of active enzymes with powerful antioxidant and anti-inflammatory effects. These ingredients work together to penetrate deep into the skin, effectively promote cell regeneration, accelerate wound healing, and reduce inflammatory responses. It has a significant repair effect on skin damage caused by sun exposure, environmental pollution, acne, burns or minor scratches. In addition, aloe vera's natural moisturizing factor can lock in skin moisture, enhance skin barrier function, improve dryness and roughness, and restore skin to softness, smoothness and elasticity. These characteristics make it one of the indispensable skin care products in many family medicine cabinets; although aloe vera has certain antibacterial ingredients, its antibacterial effect is insufficient for skin repair gels and its functionality is single. In view of this, we propose a skin repair gel based on aloe vera extract and a preparation method thereof. Summary of the invention

[0003] The object of the present invention is to provide a skin repair gel based on aloe extract and a preparation method thereof, so as to solve the problems raised in the above background technology.

[0004] To achieve the above object, on the one hand, the present invention provides a skin repair gel based on aloe extract, comprising the following components: 2-8 parts by weight of a repair agent, 33-45 parts by weight of an aloe extract, 0.5-2 parts by weight of hydroxypropyl methylcellulose, 6-9 parts by weight of glycerol, 4-8 parts by weight of sodium hyaluronate, 0.1-0.3 parts by weight of phenoxyethanol, 0.2-0.4 parts by weight of triethanolamine and 0.1-0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:5-10; The modified polyethylene glycol-polycaprolactone is prepared by modifying polyethylene glycol-polycaprolactone and 4-aminocinnamic acid in a mass ratio of 1:0.05-0.07 under the mixed conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine.

[0005] Preferably, the preparation method of the repair agent is as follows: 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution, and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 2-4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and the stirring was continued for 6-8 hours. The mixture was centrifuged. The insoluble by-products are removed, and the mixture is dried with anhydrous sodium sulfate, and then the solvent is evaporated to obtain modified polyethylene glycol-polycaprolactone; the modified polyethylene glycol-polycaprolactone is added into water, and heated and stirred at 25-35° C. to dissolve until a transparent micellar solution is formed, and curcuma dione is added into the micellar solution, and ultrasonic treatment is performed with an ultrasonic power of 30-50 W and an ultrasonic time of 20-30 min, and then the mixture is concentrated by a rotary evaporator, and the concentrated product is freeze-dried to obtain a solid powder, i.e., a repair agent.

[0006] Curcuma dione shows significant pharmacological activity in anti-inflammatory, analgesic, antithrombotic, anti-tumor, liver protection and antibacterial aspects. Since the skin repair gel of aloe extract is a hydrophilic substance, and curcuma dione is a hydrophobic substance with poor compatibility with the gel, polyethylene glycol-polycaprolactone is used to modify it. Due to the amphiphilicity of polyethylene glycol-polycaprolactone, it can self-assemble into a micelle structure in water, in which the hydrophobic part polycaprolactone forms the core, and the hydrophilic part polyethylene glycol forms the shell. The inclusion is achieved through similar hydrophobic forces, and the inclusion complex is further stabilized by hydrogen bonds and van der Waals forces, which not only protects the active ingredients, but also achieves a sustained release effect, so that curcuma dione can be slowly and continuously released to the skin, thereby providing a long-term therapeutic effect. At the same time, polyethylene glycol-polycaprolactone has good water solubility and softness, making it easier to apply, while polyethylene glycol-polycaprolactone Alcohol-polycaprolactone is electrically neutral and can only bind to the skin surface through weak non-covalent interactions. For skin repair gels, its adhesion is weak. Although the hydrophobic molecule curcumadione wrapped inside can interact with the lipid layer on the skin to a certain extent through the hydrophobic structure, thereby providing a certain adhesion force, this adhesion force is weaker than the specific adhesion force on the skin surface. Therefore, 4-aminocinnamic acid is introduced, and the carboxyl group on 4-aminocinnamic acid reacts with the hydroxyl group of polyethylene glycol-polycaprolactone to connect them. Then, the amino group on 4-aminocinnamic acid carries a positive charge in an acidic environment and forms electrostatic adsorption with the negative charge on the skin surface, thereby improving the adhesion of the inclusion complex structure on the skin surface. The additional amino and carboxyl groups can participate in the formation of more hydrogen bonds, which not only enhances the stability of the internal structure of the micelles, but also promotes close contact between the micelles and skin components.

[0007] Preferably, the concentration of the 4-aminocinnamic acid solution is 1-10 mM.

[0008] Preferably, the molar ratio of the 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:1.5-2.

[0009] Preferably, the molar ratio of the 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.2-1.4.

[0010] Preferably, the concentration of the polyethylene glycol-polycaprolactone solution is 10-20 mg / mL.

[0011] Preferably, the amount of anhydrous sodium sulfate added is 0.1-0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution.

[0012] Preferably, the concentration of the micelle solution is 3-6 mg / mL.

[0013] On the other hand, the present invention provides a method for preparing a skin repair gel based on aloe extract, which is used to prepare the above-mentioned skin repair gel based on aloe extract, comprising the following steps: Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 10-12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 5.0-6.5, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0014] Compared with the prior art, the present invention has the following beneficial effects: In the skin repair gel based on aloe extract and the preparation method thereof, a repair agent is prepared by using curcuma dione, polyethylene glycol-polycaprolactone and 4-aminocinnamic acid. Since curcuma dione has antibacterial, anti-inflammatory and analgesic effects, it is introduced into the gel, thereby improving the multifunctionality of the gel. Then, through the inclusion complexation of polyethylene glycol-polycaprolactone, the hydrophobic curcuma dione is stably dispersed in the gel structure. At the same time, the characteristic of 4-aminocinnamic acid carrying a positive charge in an acidic environment is utilized. Through the electrostatic force, the adhesion of the inclusion compound on the skin surface is improved, and the full play of the pharmacological activity of curcuma dione is promoted, thereby achieving the effect of quickly repairing damaged skin. DETAILED DESCRIPTION

[0015] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0016] The skin repair gel based on aloe extract of the present invention comprises the following components: 10-20 parts by weight of a repair agent, 60-70 parts by weight of aloe extract, 12-18 parts by weight of hydroxypropyl methylcellulose, 6-9 parts by weight of glycerol, sodium hyaluronate, phenoxyethanol, triethanolamine, butylated hydroxyanisole and 10-15 parts by weight of a penetrant; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:5-10; The modified polyethylene glycol-polycaprolactone is prepared by modifying polyethylene glycol-polycaprolactone and 4-aminocinnamic acid in a mass ratio of 1:0.05-0.07 under the mixed conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine.

[0017] Example 1: A skin repair gel based on aloe extract and a preparation method thereof, comprising the following steps: Prepare components: 8 parts by weight of repair agent, 45 parts by weight of aloe extract, 2 parts by weight of hydroxypropyl methylcellulose, 9 parts by weight of glycerin, 8 parts by weight of sodium hyaluronate, 0.3 parts by weight of phenoxyethanol, 0.4 parts by weight of triethanolamine and 0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:5; The modified polyethylene glycol-polycaprolactone is prepared by mixing polyethylene glycol-polycaprolactone with 4-aminocinnamic acid at a mass ratio of 1:0.05 under the conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine; the molar ratio of 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:2; the molar ratio of 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.4; the molar ratio of 4-aminocinnamic acid to 4-dimethylaminopyridine is 1:0.5; the amount of anhydrous sodium sulfate added is 0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution; 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution with a concentration of 5 mM, and then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution with a concentration of 10 mg / mL, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and stirring was continued. 8h, remove insoluble by-products by centrifugation, dry with anhydrous sodium sulfate, and then evaporate the solvent to obtain modified polyethylene glycol-polycaprolactone; add the modified polyethylene glycol-polycaprolactone into water, heat and stir at 30°C to dissolve until a transparent micellar solution with a concentration of 5 mg / mL is formed, add curcuma dione into the micellar solution, use ultrasonic treatment with an ultrasonic power of 50W and an ultrasonic time of 30min, and then concentrate by rotary evaporation, and then freeze-dry the concentrated product to obtain a solid powder, i.e., a repair agent; Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 6, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0018] Example 2: A skin repair gel based on aloe extract and a preparation method thereof, comprising the following steps: Prepare components: 8 parts by weight of repair agent, 45 parts by weight of aloe extract, 2 parts by weight of hydroxypropyl methylcellulose, 9 parts by weight of glycerin, 8 parts by weight of sodium hyaluronate, 0.3 parts by weight of phenoxyethanol, 0.4 parts by weight of triethanolamine and 0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:8; The modified polyethylene glycol-polycaprolactone is prepared by mixing polyethylene glycol-polycaprolactone with 4-aminocinnamic acid at a mass ratio of 1:0.06 under the conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine; the molar ratio of 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:2; the molar ratio of 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.4; the molar ratio of 4-aminocinnamic acid to 4-dimethylaminopyridine is 1:0.5; the amount of anhydrous sodium sulfate added is 0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution; 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution with a concentration of 5 mM, and then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution with a concentration of 10 mg / mL, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and stirring was continued. 8h, remove insoluble by-products by centrifugation, dry with anhydrous sodium sulfate, and then evaporate the solvent to obtain modified polyethylene glycol-polycaprolactone; add the modified polyethylene glycol-polycaprolactone into water, heat and stir at 30°C to dissolve until a transparent micellar solution with a concentration of 5 mg / mL is formed, add curcuma dione into the micellar solution, use ultrasonic treatment with an ultrasonic power of 50W and an ultrasonic time of 30min, and then concentrate by rotary evaporation, and then freeze-dry the concentrated product to obtain a solid powder, i.e., a repair agent; Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 6, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0019] Example 3: A skin repair gel based on aloe extract and a preparation method thereof, comprising the following steps: Prepare components: 8 parts by weight of repair agent, 45 parts by weight of aloe extract, 2 parts by weight of hydroxypropyl methylcellulose, 9 parts by weight of glycerin, 8 parts by weight of sodium hyaluronate, 0.3 parts by weight of phenoxyethanol, 0.4 parts by weight of triethanolamine and 0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:10; The modified polyethylene glycol-polycaprolactone is prepared by mixing polyethylene glycol-polycaprolactone with 4-aminocinnamic acid at a mass ratio of 1:0.07 under the conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine; the molar ratio of 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:2; the molar ratio of 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.4; the molar ratio of 4-aminocinnamic acid to 4-dimethylaminopyridine is 1:0.5; the amount of anhydrous sodium sulfate added is 0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution; 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution with a concentration of 5 mM, and then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution with a concentration of 10 mg / mL, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and stirring was continued. 8h, remove insoluble by-products by centrifugation, dry with anhydrous sodium sulfate, and then evaporate the solvent to obtain modified polyethylene glycol-polycaprolactone; add the modified polyethylene glycol-polycaprolactone into water, heat and stir at 30°C to dissolve until a transparent micellar solution with a concentration of 5 mg / mL is formed, add curcuma dione into the micellar solution, use ultrasonic treatment with an ultrasonic power of 50W and an ultrasonic time of 30min, and then concentrate by rotary evaporation, and then freeze-dry the concentrated product to obtain a solid powder, i.e., a repair agent; Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 6, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0020] Example 4: A skin repair gel based on aloe extract and a preparation method thereof, comprising the following steps: Prepare components: 8 parts by weight of repair agent, 33 parts by weight of aloe extract, 0.5 parts by weight of hydroxypropyl methylcellulose, 6 parts by weight of glycerin, 4 parts by weight of sodium hyaluronate, 0.1 parts by weight of phenoxyethanol, 0.2 parts by weight of triethanolamine and 0.1 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:10; The modified polyethylene glycol-polycaprolactone is prepared by mixing polyethylene glycol-polycaprolactone with 4-aminocinnamic acid at a mass ratio of 1:0.07 under the conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine; the molar ratio of 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:1.5; the molar ratio of 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.2; the molar ratio of 4-aminocinnamic acid to 4-dimethylaminopyridine is 1:0.1; the amount of anhydrous sodium sulfate added is 0.1% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution; 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution with a concentration of 5 mM, and then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution with a concentration of 10 mg / mL, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and stirring was continued. 8h, remove insoluble by-products by centrifugation, dry with anhydrous sodium sulfate, and then evaporate the solvent to obtain modified polyethylene glycol-polycaprolactone; add the modified polyethylene glycol-polycaprolactone into water, heat and stir at 30°C to dissolve until a transparent micellar solution with a concentration of 5 mg / mL is formed, add curcuma dione into the micellar solution, use ultrasonic treatment with an ultrasonic power of 50W and an ultrasonic time of 30min, and then concentrate by rotary evaporation, and then freeze-dry the concentrated product to obtain a solid powder, i.e., a repair agent; Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 6, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0021] Example 5: A skin repair gel based on aloe extract and a preparation method thereof, comprising the following steps: Prepare components: 2 parts by weight of repair agent, 45 parts by weight of aloe extract, 2 parts by weight of hydroxypropyl methylcellulose, 9 parts by weight of glycerin, 8 parts by weight of sodium hyaluronate, 0.3 parts by weight of phenoxyethanol, 0.4 parts by weight of triethanolamine and 0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:10; The modified polyethylene glycol-polycaprolactone is prepared by mixing polyethylene glycol-polycaprolactone with 4-aminocinnamic acid at a mass ratio of 1:0.07 under the conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine; the molar ratio of 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:2; the molar ratio of 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.4; the molar ratio of 4-aminocinnamic acid to 4-dimethylaminopyridine is 1:0.5; the amount of anhydrous sodium sulfate added is 0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution; 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution with a concentration of 5 mM, and then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution with a concentration of 10 mg / mL, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and stirring was continued. 8h, remove insoluble by-products by centrifugation, dry with anhydrous sodium sulfate, and then evaporate the solvent to obtain modified polyethylene glycol-polycaprolactone; add the modified polyethylene glycol-polycaprolactone into water, heat and stir at 30°C to dissolve until a transparent micellar solution with a concentration of 5 mg / mL is formed, add curcuma dione into the micellar solution, use ultrasonic treatment with an ultrasonic power of 50W and an ultrasonic time of 30min, and then concentrate by rotary evaporation, and then freeze-dry the concentrated product to obtain a solid powder, i.e., a repair agent; Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 6, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0022] Example 6: A skin repair gel based on aloe extract and a preparation method thereof, comprising the following steps: Prepare components: 5 parts by weight of repair agent, 45 parts by weight of aloe extract, 2 parts by weight of hydroxypropyl methylcellulose, 9 parts by weight of glycerin, 8 parts by weight of sodium hyaluronate, 0.3 parts by weight of phenoxyethanol, 0.4 parts by weight of triethanolamine and 0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:10; The modified polyethylene glycol-polycaprolactone is prepared by mixing polyethylene glycol-polycaprolactone with 4-aminocinnamic acid at a mass ratio of 1:0.07 under the conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine; the molar ratio of 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:2; the molar ratio of 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.4; the molar ratio of 4-aminocinnamic acid to 4-dimethylaminopyridine is 1:0.5; the amount of anhydrous sodium sulfate added is 0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution; 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution with a concentration of 5 mM, and then N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution with a concentration of 10 mg / mL, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and stirring was continued. 8h, remove insoluble by-products by centrifugation, dry with anhydrous sodium sulfate, and then evaporate the solvent to obtain modified polyethylene glycol-polycaprolactone; add the modified polyethylene glycol-polycaprolactone into water, heat and stir at 30°C to dissolve until a transparent micellar solution with a concentration of 5 mg / mL is formed, add curcuma dione into the micellar solution, use ultrasonic treatment with an ultrasonic power of 50W and an ultrasonic time of 30min, and then concentrate by rotary evaporation, and then freeze-dry the concentrated product to obtain a solid powder, i.e., a repair agent; Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 6, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

[0023] Comparative Example 1: The method of Example 3 was adopted without adding a repairing agent.

[0024] Comparative Example 2: The method of Example 3 was adopted, curcuma dione was used directly, and 4-aminocinnamic acid was not modified by modified polyethylene glycol-polycaprolactone.

[0025] Comparative Example 3: The method of Example 3 was adopted to directly modify Curcuma dione using polyethylene glycol-polycaprolactone, without modifying the polyethylene glycol-polycaprolactone using 4-aminocinnamic acid.

[0026] The present invention adopts a repair agent to prepare a skin repair gel based on aloe extract, wherein the performance index inspection items and inspection standards of the skin repair gel based on aloe extract are as follows: The lap shear strength test is designed to evaluate the maximum stress that two overlapping surfaces can withstand under the shear force parallel to the contact surface until the bond fails; the gel is evenly spread on the pig skin to form an area of ​​25mm×25mm and 1mm thick, and another piece of pig skin is covered on the surface coated with the gel to ensure that the two substrates are completely aligned and in full contact. The experimental temperature is set to 37℃, the standard lap length is set to 12.5mm, and a tensile tester is used to apply a tensile force parallel to the bonding surface to the sample. The test is carried out at a speed of 5mm / min. The maximum shear force is tested and the lap shear strength (Kpa) is calculated as follows: maximum shear force (N) / lap area (mm 2 ).

[0027] Antimicrobial tests included anti-Staphylococcus aureus and Propionibacterium acnes, diluted to 1 × 10 8 CFU / mL, use a sterile cotton swab to dip the above bacterial suspension, evenly spread it on the surface of the agar plate to ensure that the entire surface of the plate is covered, use a sterile hole puncher to punch a small hole with a diameter of 6 mm on the agar plate, add 20 μL of gel sample to each hole, and set up a blank control group at the same time, place all treated agar plates in a 37°C constant temperature incubator and incubate for 24 hours. After the incubation, measure the diameter of the transparent inhibition zone formed around each hole and record the data. The larger the inhibition zone, the stronger the antibacterial effect.

[0028] According to the above standards, the skin repair gel based on aloe extract prepared in the above Examples 1-6 and Comparative Examples 1-3 was tested, and the obtained data are shown in Table 1: Table 1 Performance data of Examples 1-6 and Comparative Examples 1-3 The above data fully show that compared with Comparative Examples 1-3, Examples 1-6 can fully show the effect of the repair agent on the antibacterial and adhesion properties of the skin repair gel based on aloe extract.

[0029] Since the present invention uses a repair agent to prepare the skin repair gel based on aloe extract, the performance of the skin repair gel is effectively improved by the repair agent, as follows: It can be seen from Examples 1-3 that with the continuous increase in the proportion of the repair agent component, the antibacterial and adhesion properties of the skin repair gel based on aloe extract are significantly improved, indicating that the modified polyethylene glycol-polycaprolactone is not only amphiphilic and can stably include the hydrophobic curcuma dione in its internal hydrophobic core, but can also increase its electrostatic adsorption ability on the skin surface by introducing 4-aminocinnamic acid. When the proportion of modified polyethylene glycol-polycaprolactone increases, it means that the inclusion effect of curcuma dione is better, so that curcuma dione is stably dispersed in the gel, thereby improving the antibacterial effect of curcuma dione. At the same time, 4-aminocinnamic acid can also be better linked to polyethylene glycol-polycaprolactone, improving the electrostatic adsorption effect, and then improving the adhesion.

[0030] It can be seen from Examples 2 and 4 that with the continuous change of the content of other components, the antibacterial and adhesion properties of the skin repair gel based on aloe extract do not change significantly, indicating that small changes in other components within a certain range are not sufficient to significantly affect the antibacterial and adhesion properties of the skin repair gel based on aloe extract.

[0031] It can be seen from Examples 2, 5 and 6 that as the content of the repair agent continues to change, the antibacterial and adhesion properties of the skin repair gel based on aloe extract continue to change, which illustrates the effective antibacterial effect of curcuma dione. When the content of the repair agent increases, it means that more active ingredients can reach the skin surface, enhancing the antibacterial effect. In addition, since 4-aminocinnamic acid in the repair agent provides additional adhesion, the increase in the content of the repair agent also directly improves the adhesion performance of the gel.

[0032] According to the above test experiments, the skin repair gel based on aloe extract prepared according to Example 3 has the best performance, so Example 3 is taken as the best example; By comparing Example 3 with Comparative Examples 1-3, it can be seen that: In Comparative Example 1, no repair agent was added. The antibacterial and adhesion properties of the skin repair gel based on aloe extract were worse, indicating that the repair agent had significant antibacterial activity. When the repair agent was not added, the gel lacked this important antibacterial ingredient, resulting in a significant weakening of its antibacterial effect. In addition, the repair agent not only contained active ingredients, but also enhanced the adhesion of the gel to the skin surface through modified polyethylene glycol-polycaprolactone and 4-aminocinnamic acid. Therefore, in the absence of the repair agent, the adhesion of the gel to the skin decreased, affecting the drug delivery efficiency.

[0033] In Comparative Example 2, curcumadione was directly used, and 4-aminocinnamic acid was not modified by modified polyethylene glycol-polycaprolactone. The antibacterial and adhesion properties of the skin repair gel based on aloe extract were poor. This is because curcumadione is a hydrophobic substance, and the gel matrix is ​​usually hydrophilic. Unmodified curcumadione is difficult to disperse evenly in the gel and is prone to aggregate or precipitation, which not only affects its stability and long-term release, but also reduces its antibacterial efficacy. In addition, unmodified curcumadione itself does not have the function of enhancing the adhesion of the gel. Therefore, the durability and efficacy of the gel applied on the skin are limited.

[0034] In Comparative Example 3, polyethylene glycol-polycaprolactone was directly used to modify curcumadione, and polyethylene glycol-polycaprolactone was not modified by 4-aminocinnamic acid. The antibacterial and adhesion properties of the skin repair gel based on aloe extract were poor. On the one hand, it shows that polyethylene glycol-polycaprolactone can help curcumadione to be better dispersed in the gel and provide a certain sustained-release effect. On the other hand, it shows the role of 4-aminocinnamic acid in improving the skin adhesion of the gel, and can achieve the purpose of prolonging the residence time of the drug on the skin by enhancing the electrostatic adsorption between the gel and the skin surface. The lack of 4-aminocinnamic acid means that the gel loses this important adhesion enhancement mechanism, thereby affecting the antibacterial and adhesion effects.

[0035] In summary, the use of a repair agent, taking advantage of the fact that curcuma dione has multiple pharmacologically active ingredients, the self-assembly properties of polyethylene glycol-polycaprolactone and the charged properties of 4-aminocinnamic acid, allows the effective loading and sustained release of curcuma dione to ensure the gel's strong inhibitory ability against pathogenic microorganisms, and 4-aminocinnamic acid gives the gel better skin adhesion, allowing the drug to stay more firmly in the affected area, prolonging the action time, improving the drug's absorption efficiency, and ensuring the maximization of the drug's efficacy, thereby effectively repairing damaged skin tissue.

[0036] The above shows and describes the basic principles, main features and advantages of the present invention. Those skilled in the art should understand that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only preferred examples of the present invention, and are not intended to limit the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A skin repair gel based on aloe extract, characterized in that: The invention comprises the following components: 2-8 parts by weight of a repair agent, 33-45 parts by weight of an aloe extract, 0.5-2 parts by weight of hydroxypropyl methylcellulose, 6-9 parts by weight of glycerin, 4-8 parts by weight of sodium hyaluronate, 0.1-0.3 parts by weight of phenoxyethanol, 0.2-0.4 parts by weight of triethanolamine and 0.1-0.5 parts by weight of butylated hydroxyanisole; The repair agent is prepared by mixing curcuma dione and modified polyethylene glycol-polycaprolactone in a mass ratio of 1:5-10; The modified polyethylene glycol-polycaprolactone is prepared by modifying polyethylene glycol-polycaprolactone and 4-aminocinnamic acid in a mass ratio of 1:0.05-0.07 under the mixed conditions of N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and 4-dimethylaminopyridine.

2. The skin repair gel based on aloe extract according to claim 1, characterized in that: The preparation method of the repair agent is as follows: 4-aminocinnamic acid was dissolved in dichloromethane to form a 4-aminocinnamic acid solution, and N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide were added, and the mixed solution was stirred at room temperature for 2-4 hours. Polyethylene glycol-polycaprolactone was dissolved in the same solvent dichloromethane to prepare a polyethylene glycol-polycaprolactone solution, and then the mixed solution was added dropwise to the polyethylene glycol-polycaprolactone solution, and 4-dimethylaminopyridine was added, and the stirring was continued for 6-8 hours. The mixture was centrifuged. The insoluble by-products are removed, and the mixture is dried with anhydrous sodium sulfate, and then the solvent is evaporated to obtain modified polyethylene glycol-polycaprolactone; the modified polyethylene glycol-polycaprolactone is added into water, and heated and stirred at 25-35° C. to dissolve until a transparent micellar solution is formed, and curcuma dione is added into the micellar solution, and ultrasonic treatment is performed with an ultrasonic power of 30-50 W and an ultrasonic time of 20-30 min, and then the mixture is concentrated by a rotary evaporator, and the concentrated product is freeze-dried to obtain a solid powder, i.e., a repair agent.

3. The skin repair gel based on aloe extract according to claim 2, characterized in that: The concentration of the 4-aminocinnamic acid solution is 1-10 mM.

4. The skin repair gel based on aloe extract according to claim 2, characterized in that: The molar ratio of the 4-aminocinnamic acid to N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride is 1:1.5-2.

5. The skin repair gel based on aloe extract according to claim 2, characterized in that: The molar ratio of the 4-aminocinnamic acid to N-hydroxysuccinimide is 1:1.2-1.

4.

6. The skin repair gel based on aloe extract according to claim 2, characterized in that: The concentration of the polyethylene glycol-polycaprolactone solution is 10-20 mg / mL.

7. The skin repair gel based on aloe extract according to claim 2, characterized in that: The molar ratio of the 4-aminocinnamic acid to the 4-dimethylaminopyridine is 1:0.1-0.

5.

8. The skin repair gel based on aloe extract according to claim 2, characterized in that: The addition amount of the anhydrous sodium sulfate is 0.1-0.2% of the total volume of the polyethylene glycol-polycaprolactone solution and the mixed solution.

9. The skin repair gel based on aloe extract according to claim 2, characterized in that: The concentration of the micelle solution is 3-6 mg / mL.

10. A method for preparing a skin repair gel based on aloe extract, for preparing the skin repair gel based on aloe extract according to any one of claims 1 to 9, characterized in that: The steps include: Disperse hydroxypropyl methylcellulose in distilled water, stir evenly, let stand to swell for 10-12 hours, then add glycerin, sodium hyaluronate and repair agent, stir until completely mixed to ensure uniform distribution, add the above mixed solution to aloe extract, use a homogenizer to continuously stir, and add triethanolamine to adjust the pH of the aloe extract to 5.0-6.5, finally add phenoxyethanol and butylated hydroxyanisole, and after sufficient mixing, obtain a skin repair gel based on aloe extract.

Images