A detachable modular microfluidic chip

Abstract

The present invention discloses a detachable modular microfluidic chip, comprising an upper clamping plate, a chip, a transparent substrate and a lower clamping plate which are stacked in sequence from top to bottom and detachably connected, as well as a valve body, a fixing cap, a transparent observation piece and a sealing gasket; the chip is provided with multiple sections of flow channels which penetrate the bottom surface of the chip and multiple valve body interfaces on the flow channels, the upper clamping plate is provided with a valve body fixing hole, the lower end of the valve body is detachably connected to the valve body interface, the upper end is mounted on the valve body fixing hole, the top surface of the valve body fixing hole is higher than the top surface of the upper clamping plate, the fixing cap is connected to the valve body fixing hole, and a hole for inserting an inlet and outlet sample hose is reserved on the fixing cap; an upper observation hole is also provided on the upper clamping plate, an observation hole is provided at a position corresponding to the upper observation hole on the chip, a light source hole is provided at a position corresponding to the observation hole on the lower clamping plate, and a transparent observation piece is arranged in the observation hole of the chip and sealed with a sealing gasket. The detachable modular microfluidic chip of the present invention realizes the efficient, flexible and low-cost use of the microfluidic chip.

Description

Technical Field

[0001] The invention relates to microfluidic technology, in particular to a detachable modular microfluidic chip. Background Art

[0002] Since its introduction in the 1990s, microfluidic technology has been widely used in biomedicine, chemical analysis and other fields. Microfluidic chips have become an important tool for complex biochemical experiments due to their miniaturization, high efficiency and low consumption. Although traditional microfluidic chips are highly integrated, their design and processing are complex and require professional micro-processing equipment and technicians, which limits their application in a wider range of fields.

[0003] The research and application of modular microfluidic systems are growing day by day. Their advantages are flexible combination, strong adaptability, and the ability to significantly reduce the processing and use costs of microfluidic chips through mass production. However, the development of modular microfluidic chips is still in its early stages. Various materials, processing methods, and interface technologies have emerged one after another, and a standardized modular microfluidic technology system has not yet been formed. In addition, the design and performance research of microvalve of microfluidic chips shows that although mechanical rotary microvalve has the characteristics of high reliability, strong versatility and low cost, its structure is complex and may affect the overall performance and service life of the chip.

[0004] Although disposable microfluidic devices simplify the operation process and reduce costs, their non-reusability limits their flexibility and economy in certain application scenarios. Although portable microfluidic dispensing systems can be manually operated and easily reused, their functions are relatively simple and cannot meet the needs of complex experiments. Although the flexibility and cost-effectiveness of modular microfluidic systems have been recognized, their low degree of standardization limits the compatibility and interchangeability between different modules. Secondly, although existing studies have proposed a variety of microvalve design schemes, these designs are often targeted at specific application scenarios and lack versatility and adaptability. In addition, although disposable microfluidic devices simplify the operation process, their non-reusability limits their economy and sustainability in certain application scenarios.

[0005] It should be noted that the information disclosed in the above background technology section is only used for understanding the background of the present application, and therefore may include information that does not constitute prior art known to ordinary technicians in the field. Summary of the invention

[0006] In order to make up for the deficiencies of the prior art, the present invention provides a detachable modular microfluidic chip.

[0007] The present invention adopts the following technical solution:

[0008] A detachable modular microfluidic chip, comprising an upper clamping plate, a chip, a transparent substrate and a lower clamping plate which are stacked in sequence from top to bottom and are detachably connected; further comprising a valve body, a fixing cap, a transparent observation plate and a sealing gasket; the chip is provided with a plurality of flow channels and a plurality of valve body interfaces, the flow channels pass through the bottom surface of the chip, the valve body interface is arranged on the top surface of the chip and is located on the flow channels, a valve body fixing hole is arranged on the upper clamping plate at a position corresponding to the valve body interface, and the lower end of the valve body is detachably connected to the valve body interface on the chip to communicate For each flow channel required for the experiment, the upper end of the valve body is installed on the valve body fixing hole on the upper clamping plate, the top surface of the valve body fixing hole is higher than the top surface of the upper clamping plate, the fixing cap is connected to the valve body fixing hole, and a hole for inserting the inlet and outlet sample hose is reserved on the fixing cap; an upper observation hole is also provided on the upper clamping plate, an observation hole is provided at a position corresponding to the upper observation hole on the chip, a light source hole is provided at a position corresponding to the observation hole on the lower clamping plate, and the transparent observation sheet is arranged in the observation hole of the chip and sealed with the sealing gasket.

[0009] Furthermore, the bottom surface of the upper clamping plate has an upper groove for accommodating the chip and matching the shape of the chip, and the top surface of the lower clamping plate has a lower groove for accommodating the transparent substrate and matching the shape of the transparent substrate; the sum of the groove depths of the upper groove and the lower groove is less than the sum of the thicknesses of the chip and the transparent substrate.

[0010] Furthermore, the sum of the depths of the upper groove and the lower groove is 0.2-0.5 mm smaller than the sum of the thicknesses of the chip and the transparent substrate.

[0011] Furthermore, the groove depth of the upper groove is smaller than the thickness of the chip; the groove depth of the lower groove is larger than the thickness of the transparent substrate and smaller than the sum of the thickness of the chip and the transparent substrate.

[0012] Furthermore, a first positioning portion is provided on the circumference of the valve body between the end portion of the lower end of the valve body and the end portion of the upper end of the valve body, and a second positioning portion cooperating with the first positioning portion is provided on the circumference of the inner wall of the valve body interface, and the detachable connection between the lower end of the valve body and the valve body interface on the chip is achieved through the cooperation of the first positioning portion and the second positioning portion.

[0013] Further, the first positioning portion is a protrusion or a groove, and correspondingly, the second positioning portion is a groove or a protrusion. Further, the first positioning portion is a protrusion, and the second positioning portion is a groove.

[0014] Furthermore, the height of the valve body satisfies that: when the upper clamping plate, the chip, the transparent substrate, the lower clamping plate and the valve body are assembled, the end surface of the upper end of the valve body is higher than the upper surface of the valve body fixing hole, so that the fixing cap can pressurize and fix the valve body.

[0015] Furthermore, the outer surface of the valve body fixing hole is provided with an external thread, and the fixing cap is provided with an internal thread matching with the external thread.

[0016] Furthermore, threaded holes for bolt installation are provided in the area of ​​the upper clamping plate where the upper groove is not provided and in the area of ​​the lower clamping plate where the lower groove is not provided, and the upper clamping plate and the lower clamping plate are detachably connected by bolts.

[0017] Furthermore, the lower end of the valve body has a predetermined channel shape, so that the valve body includes at least one of a straight two-way valve, a 90-degree two-way valve, a T-type three-way valve, a cross four-way valve and an inlet / outlet sample valve.

[0018] Furthermore, the chip is a photocurable chip; the valve body is a photocurable valve body; the transparent substrate is made of glass; and the transparent observation piece is made of glass.

[0019] The present invention has the following beneficial effects:

[0020] 1. The detachable modular microfluidic chip of the present invention adopts a modular design. Each module can be independently designed, manufactured and modified, and the modules can be detachably connected, which not only reduces the manufacturing cost, but also improves the adaptability and flexibility of the microfluidic chip. By setting the upper observation hole on the upper clamping plate, the corresponding observation hole on the chip and the corresponding light source hole on the lower clamping plate, it is convenient for users to integrate the detachable modular microfluidic chip into observation equipment such as a microscope to observe the inside of the chip in real time, thereby improving the efficiency and accuracy of the experiment, and making the application range of the chip more extensive and suitable for a variety of experimental environments.

[0021] 2. The detachable modular microfluidic chip of the present invention can be thoroughly cleaned and reused after use, which overcomes the problem that traditional microfluidic chips are difficult to clean, improves the reuse rate of the microfluidic chip and prolongs the service life of the chip.

[0022] 3. In the preferred technical solution, by designing the valve body, the user can quickly use the valve body to switch different flow channels of the chip as needed, realizing the function of one chip for multiple uses, that is, multiple groups of experimental conditions can be considered in the early stage of design, and there is no need to re-prepare the chip due to different experimental conditions. This design simplifies the operation steps and improves the experimental efficiency.

[0023] Therefore, the disassembly modular microfluidic chip of the present invention realizes efficient, flexible and low-cost use of the microfluidic chip, and has significant technical effects and broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 is an exploded schematic diagram of a detachable modular microfluidic chip in a specific embodiment of the present invention;

[0025] Figure 2 is a schematic diagram of an assembled detachable modular microfluidic chip in a specific embodiment of the present invention;

[0026] Figure 3 is a schematic diagram of a chip in a specific embodiment of the present invention;

[0027] Figure 4 yes Figure 3 An enlarged schematic diagram of the middle valve body interface 31;

[0028] Figure 5 is a bottom view schematic diagram of a chip in a specific embodiment of the present invention;

[0029] Figure 6-10 They are schematic diagrams of the structures of valve bodies with five different functions in specific embodiments of the present invention;

[0030] Fig.11 Schematic diagram of the chip structure in the experimental example of the present invention. DETAILED DESCRIPTION

[0031] The following is a detailed description of the embodiments of the present invention. It should be emphasized that the following description is only exemplary and is not intended to limit the scope and application of the present invention. In the absence of conflict, the embodiments and features in the embodiments of the present application can be combined with each other.

[0032] It should be noted that when an element is referred to as being "fixed to" or "disposed on" another element, it can be directly on the other element or indirectly on the other element. When an element is referred to as being "connected to" another element, it can be directly connected to the other element or indirectly connected to the other element. In addition, connection can be used for fixing as well as for coupling or communication.

[0033] It should be understood that the terms "length", "width", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inside", "outside", etc., indicating the orientation or position relationship, are based on the orientation or position relationship shown in the accompanying drawings, and are only for the convenience of describing the embodiments of the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore cannot be understood as a limitation on the present invention.

[0034] The specific embodiment of the present invention provides a detachable modular microfluidic chip, such as Figure 1-5 As shown, it includes an upper clamping plate 1, a chip 3, a transparent substrate 4 and a lower clamping plate 2 which are stacked in sequence from top to bottom and are detachably connected; it also includes a valve body 6, a fixing cap 5, a transparent observation piece 8 and a sealing gasket 9; the chip 3 is provided with multiple flow channels 33 (such as Figure 5 In the example shown, multiple sections of flow channels 33 are in a grid shape) and multiple valve body interfaces 31, the flow channels 33 pass through the bottom surface of the chip 3, the valve body interface 31 is arranged on the top surface of the chip 3 and is located on the flow channels 33, a valve body fixing hole 11 is arranged on the upper clamping plate 1 at a position corresponding to the valve body interface 31, the lower end of the valve body 6 is detachably connected to the valve body interface 31 on the chip 3 to connect the flow channels required for the experiment, the upper end of the valve body 6 is installed on the valve body fixing hole 11 on the upper clamping plate 1, and the top of the valve body fixing hole 11 The surface is higher than the top surface of the upper clamping plate 1 (the valve body fixing hole 11 is a column extending upward from the top surface of the upper clamping plate 1), the fixing cap 5 is connected to the valve body fixing hole 11, and the fixing cap 5 is reserved with a hole for inserting the inlet and outlet sample hose 7; the upper clamping plate 1 is also provided with an upper observation hole 12, the chip 3 is provided with an observation hole 32 at a position corresponding to the upper observation hole 12, the lower clamping plate 2 is provided with a light source hole 22 at a position corresponding to the observation hole 32, and the transparent observation sheet 8 is arranged in the observation hole 32 of the chip 3 and is sealed with the sealing gasket 9.

[0035] Through the detachable modular microfluidic chip in the above specific implementation, the disassembly or connection between the modules can be facilitated, the manufacturing cost is reduced, and the adaptability and flexibility of the microfluidic chip are improved. The detachable modular microfluidic chip can be thoroughly cleaned and reused after use, which overcomes the problem that the traditional microfluidic chip is difficult to clean, improves the reuse rate of the microfluidic chip and prolongs the service life of the chip. Through the upper observation hole set on the upper clamping plate, the corresponding observation hole set on the chip and the corresponding light source hole set on the lower clamping plate, it is convenient for users to integrate the detachable modular microfluidic chip into observation equipment such as a microscope to observe the inside of the chip in real time (for example, observe the control effect of the chip on the fluid or the behavior state of other substances, such as the mixing effect of the reagents in the detection or the behavior state of particles such as fluorescent microspheres and microorganisms in the fluid under the flow control experiment), thereby improving the efficiency and accuracy of the experiment, and making the application range of the chip wider and suitable for a variety of experimental environments.

[0036] The size of the upper and lower clamps is designed to be well integrated and fixed with the mainstream optical microscope observation platform on the market (for example, the adjustable range of the microscope stage clamp used in the present invention is between 20 and 120 mm, and the length and width of the upper and lower clamps are also designed to be between 20 and 120 mm to match the microscope stage). The upper observation hole set on the upper clamp, the corresponding observation hole set on the chip, and the corresponding light source hole set on the lower clamp can ensure that when the detachable modular microfluidic chip is integrated into the observation equipment (such as an optical microscope), the optical microscope objective lens can be inserted, and the minimum distance between the objective lens and the sample can be reduced to adapt to the objective lens with higher magnification. The light source hole also facilitates sufficient light source to assist observation under the observation conditions of a small field of view.

[0037] In the above embodiment, the flow channel 33 passes through the bottom surface of the chip 3. After the detachable modular microfluidic chip is assembled, the sealing of the flow channel is ensured by sealing the top surface of the transparent substrate 4 with the bottom surface of the chip 3. At the same time, after disassembling the modular microfluidic chip, it is more conducive to thoroughly cleaning the chip.

[0038] In such Figure 1-5In the example shown, there are 10 valve body fixing holes 11. In this example, only 6 valve bodies 6 and 6 fixing caps 5 are assembled for illustration. In practice, the number of valve body fixing holes 11 can be set as needed, and the number of valve bodies 6 and fixing caps 5 to be assembled can be selected according to experimental needs. In some embodiments, the bottom surface of the upper clamping plate 1 has an upper groove (not shown, refer to the lower groove 21 on the lower clamping plate 2) for accommodating the chip 3 and matching the shape of the chip 3, and the top surface of the lower clamping plate 2 has a lower groove 21 for accommodating the transparent substrate 4 and matching the shape of the transparent substrate 4; the sum of the groove depths of the upper groove and the lower groove 21 is less than the sum of the thicknesses of the chip 3 and the transparent substrate 4. Figure 1 In the example shown, both the upper groove and the lower groove are square, but not limited thereto, and the shapes of the upper groove and the lower groove depend on the shapes of the chip 3 and the transparent substrate 4. By accommodating the transparent substrate 4 and the chip 3 in the cavity between the upper groove of the upper clamping plate 1 and the lower groove of the lower clamping plate 2, horizontal slippage of the transparent substrate 4 and the chip 3 can be avoided during observation. The sum of the groove depths of the upper groove and the lower groove 21 is less than the sum of the thicknesses of the chip 3 and the transparent substrate 4 to ensure that there is sufficient pressure between the chip and the transparent substrate after assembly to ensure sealing. In some embodiments, the sum of the groove depths of the upper groove and the lower groove 21 is 0.2-0.5 mm less than the sum of the thicknesses of the chip 3 and the transparent substrate 4.

[0039] In some embodiments, the groove depth of the upper groove is less than the thickness of the chip 3 ; the groove depth of the lower groove 21 is greater than the thickness of the transparent substrate 4 and less than the sum of the thicknesses of the chip 3 and the transparent substrate 4 .

[0040] In some embodiments, the inner wall of the observation hole 32 on the chip 3 is a slope that gradually narrows from top to bottom, which is convenient for the placement of the transparent observation piece 8. At the same time, in order to improve the reliability of the seal, a sealing gasket 9 is added when the transparent observation piece 8 is assembled into the observation hole 32. The material and size of the sealing gasket 9 are selected based on the basic principle of ensuring sealing without crushing the chip body.

[0041] In some embodiments, the height of the valve body 6 satisfies: when the upper clamping plate 1, the chip 3, the transparent substrate 4, the lower clamping plate 2 and the valve body 6 are assembled, the end surface of the upper end of the valve body 6 is higher than the upper surface of the valve body fixing hole 11, so that the fixing cap 5 can pressurize and fix the valve body 6.

[0042] In some embodiments, the outer surface of the valve body fixing hole 11 is provided with an external thread, and the fixing cap 5 is provided with an internal thread matching the external thread. The valve body fixing hole 11 and the fixing cap 5 are threadedly matched to facilitate installation of the valve body after assembly.

[0043] In some embodiments, the area of ​​the upper clamping plate 1 without the upper groove and the area of ​​the lower clamping plate 2 without the lower groove are provided with threaded holes 13, 23 for bolt installation, and the upper clamping plate 1 and the lower clamping plate 2 are detachably connected by bolts. The upper and lower clamping plates are fastened by screws, which makes it easier to disassemble the components.

[0044] In some embodiments, further reference is made to Figure 4 and Figure 6-10 A first positioning portion 61 is provided on the circumference of the valve body between the end portion of the lower end of the valve body 6 and the end portion of the upper end of the valve body 6, and a second positioning portion 311 cooperating with the first positioning portion 61 is provided on the circumference of the inner wall of the valve body interface 31. The cooperation between the first positioning portion 61 and the second positioning portion 311 realizes a detachable connection between the lower end of the valve body 6 and the valve body interface 31 on the chip.

[0045] In some embodiments, the first positioning portion 61 is a protrusion or a groove, and correspondingly, the second positioning portion 311 is a groove or a protrusion; further preferably, the first positioning portion 61 is a protrusion, and the second positioning portion 311 is a groove, and the detachable connection between the valve body and the chip is achieved through the interference fit between the protrusion and the groove. In this example, four protrusions are evenly arranged on the circumference of the valve body, and correspondingly, four grooves are evenly arranged on the circumference of the inner wall of the valve body interface 31.

[0046] In some embodiments, Figure 6-10 As shown in FIG. 1 , it is a schematic diagram of the structure of five valve bodies with different functions. The end of the lower end of the valve body 6 has a predetermined channel shape, so that the valve body includes a straight two-way valve (such as Figure 6 As shown), 90 degree two-way valve (as Figure 7 As shown), T-type three-way valve (as Figure 8 As shown), cross four-way valve (as Fig. 9 as shown) and inlet / outlet valves (as shown Fig.10 In the experiment, according to the experimental needs, a variety of valve bodies can be used in combination.

[0047] In some embodiments, the chip is a photocurable chip; the valve body is a photocurable valve body; the transparent substrate is made of glass; and the transparent observation piece is made of glass.

[0048] Among them, the chip and the valve body can be made of photocurable resin materials and manufactured through photocuring molding technology. The molding accuracy can reach 10 microns and the flatness can reach 1 micron. In the specific design process of the valve body, it is necessary to calculate a reasonable assembly accuracy and select a reasonable manufacturing tolerance to achieve a better sealing effect. The material selection of the upper and lower splints should meet certain strength requirements to avoid excessive deformation causing uneven pressure distribution between the transparent substrate and the chip affecting the sealing effect. The upper splint 1, the lower splint 2 and the fixing cap can be manufactured using 3D printing technology. The specific material can be selected according to the use scenario considering factors such as corrosion resistance and mechanical strength. If necessary, machining technologies with wider material adaptability such as milling, turning, grinding, laser processing, electroplating, anodizing and other machining or surface treatment technologies can be used to process metal components to adapt to more special use environments.

[0049] The present invention is further described below through experimental examples.

[0050] Experimental example: Chemotaxis of aquatic microorganisms to nitrogen and phosphorus nutrients

[0051] like Fig.11 As shown, the positions of the valve body interface 31 used in the experiment are a, b, c, e, f, j, h, i, wherein the valve bodies 6 connected at each position are specifically: the valve body interfaces at a, c, h, i, and j are connected to the inlet / outlet sample valve, the valve body interface at e is connected to the T-type three-way valve, the valve body interface at b is prepared to prepare a 90-degree two-way valve and a T-type three-way valve, and the valve body interface at j is connected to a straight two-way valve; the inlet / outlet sample valve connecting hose at a is connected to the nitrogen salt solution, the inlet / outlet sample valve connecting hose at c is connected to the phosphate solution, the inlet / outlet sample valve connecting hose at h is connected to the physiological saline, the inlet / outlet sample valve at j is connected to the waste liquid collection container, and the inlet / outlet sample valve at i is connected to the microbial solution (as shown by the black arrow).

[0052] The first group of experiments: studying the chemotaxis of microorganisms to nitrogen nutrients. At this time, the valve body interface at b is connected to a 90-degree two-way valve to connect a and e. The streamline is as follows Fig.11 As shown by the red line in the middle. At this time, the nitrogen salt solution and the physiological saline converge at e, and under the mixing action of the T-shaped channel at e, a concentration gradient is formed. The fluid in the microchannel is in a laminar state, so the concentration gradient in the liquid is still retained at the observation port. At the same time, during the process from f to j, the microorganisms can be observed through a microscope at the observation port to swim towards the concentration gradient direction that is more conducive to their growth due to their own chemotaxis characteristics.

[0053] The second group of experiments: to study the chemotaxis of microorganisms to phosphorus nutrients. At this time, the 90-degree two-way valve at b is turned to connect c and e. The streamline is as follows Fig.11 The yellow line in the middle shows the experimental process and principle. The experimental process and principle are the same as the first group of experiments.

[0054] The third group of experiments: study the chemotaxis of microorganisms under the combined action of nitrogen and phosphorus nutrients. At this time, the valve body interface at b is connected to the T-type three-way valve to connect a, c and e. The streamlines are shown as blue lines in the figure. Adjusting the flow rate of the two inlets at a and c can control the ratio of nitrogen salts and phosphorus salts. At the same time, the mixed nutrients are mixed with physiological saline at e to generate a concentration gradient. The subsequent experimental process is the same as above.

[0055] It should be noted that if the effect of changing the dynamic process of nutrient environment on microbial behavior is studied, it is only necessary to switch the valve body. If there is a need to control variables, the chip needs to be disassembled and cleaned between each group of experiments. Nevertheless, it can ensure that each group of experiments is carried out in the same physical environment, which improves the accuracy of the experiment.

[0056] The above content is a further detailed description of the present invention in combination with specific / preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, without departing from the concept of the present invention, it can also make several substitutions or modifications to these described embodiments, and these substitutions or modifications should be regarded as belonging to the protection scope of the present invention. In the description of this specification, the description of the reference terms "an embodiment", "some embodiments", "preferred embodiments", "examples", "specific examples", or "some examples" means that the specific features, structures, materials or characteristics described in combination with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily target the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner. In the absence of mutual contradiction, those skilled in the art can combine and combine the different embodiments or examples described in this specification and the features of different embodiments or examples. Although the embodiments of the present invention and their advantages have been described in detail, it should be understood that various changes, substitutions and modifications can be made herein without departing from the scope of protection of the patent application.

Claims

1. A detachable modular microfluidic chip, characterized in that: The invention comprises an upper clamping plate, a chip, a transparent substrate and a lower clamping plate which are stacked in sequence from top to bottom and are detachably connected. The bottom surface of the upper clamping plate has an upper groove which accommodates the chip and matches the shape of the chip, and the top surface of the lower clamping plate has a lower groove which accommodates the transparent substrate and matches the shape of the transparent substrate; the sum of the groove depths of the upper groove and the lower groove is less than the sum of the thicknesses of the chip and the transparent substrate; the invention also comprises a valve body, a fixing cap, a transparent observation sheet and a sealing gasket; The chip is provided with multiple flow channels and multiple valve body interfaces, the flow channels pass through the bottom surface of the chip, the valve body interface is provided on the top surface of the chip and is located on the flow channels, a valve body fixing hole is provided at a position on the upper clamping plate corresponding to the valve body interface, the lower end of the valve body is detachably connected to the valve body interface on the chip to connect various flow channels required for the experiment, the upper end of the valve body is mounted on the valve body fixing hole on the upper clamping plate, the top surface of the valve body fixing hole is higher than the top surface of the upper clamping plate, the fixing cap is connected to the valve body fixing hole, and a hole for inserting the inlet and outlet sample hose is reserved on the fixing cap; The upper clamping plate is also provided with an upper observation hole, an observation hole is provided at a position corresponding to the upper observation hole on the chip, a light source hole is provided at a position corresponding to the observation hole on the lower clamping plate, and the transparent observation sheet is arranged in the observation hole of the chip and sealed with the sealing gasket; A protrusion is provided on the circumference of the valve body between the end of the lower end of the valve body and the end of the upper end of the valve body, and a groove cooperating with the protrusion is provided on the circumference of the inner wall of the valve body interface, and the detachable connection between the lower end of the valve body and the valve body interface is achieved through the cooperation of the protrusion and the groove; The height of the valve body satisfies that: after the upper clamping plate, the chip, the transparent substrate, the lower clamping plate and the valve body are assembled, the end surface of the upper end of the valve body is higher than the upper surface of the valve body fixing hole, so that the fixing cap can pressurize and fix the valve body.

2. The detachable modular microfluidic chip according to claim 1, characterized in that: The sum of the groove depths of the upper groove and the lower groove is 0.2-0.5 mm smaller than the sum of the thicknesses of the chip and the transparent substrate.

3. The detachable modular microfluidic chip according to claim 1, characterized in that: The groove depth of the upper groove is less than the thickness of the chip; the groove depth of the lower groove is greater than the thickness of the transparent substrate and less than the sum of the thicknesses of the chip and the transparent substrate.

4. The detachable modular microfluidic chip according to claim 1, characterized in that: The outer surface of the valve body fixing hole is provided with an external thread, and the fixing cap is provided with an internal thread matching with the external thread.

5. The detachable modular microfluidic chip according to claim 1, characterized in that: The area of ​​the upper clamping plate without the upper groove and the area of ​​the lower clamping plate without the lower groove are provided with threaded holes for bolt installation, and the upper clamping plate and the lower clamping plate are detachably connected by bolts.

6. The detachable modular microfluidic chip according to claim 1, characterized in that: The lower end of the valve body has a predetermined channel shape, so that the valve body includes at least one of a straight two-way valve, a 90-degree two-way valve, a T-type three-way valve, a cross four-way valve and an inlet / outlet sample valve.

7. The detachable modular microfluidic chip according to claim 1, characterized in that: The chip is a light-cured chip; the valve body is a light-cured valve body; the transparent substrate is made of glass; and the transparent observation piece is made of glass.

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