Separation and extraction method of tea fuzz, tea polysaccharide and application of tea polysaccharide

Abstract

The invention provides a separation and extraction method of tea fuzz, tea polysaccharide and application thereof. The separation and extraction method of the tea fuzz comprises the following steps: S1, quick-freezing fresh tea leaves through liquid nitrogen to obtain a pre-frozen tea sample; s2, crushing and sieving the pre-frozen tea sample, and collecting tealeaf fuzz; and S3, soaking the tea fuzz with saline water for 20-30 minutes, extracting with hot water, filtering, carrying out alcohol precipitation, centrifuging, separating out precipitate, carrying out deproteinization treatment on the precipitate, dialyzing and freeze-drying to obtain the tea polysaccharide in the tea fuzz. According to the method, the extraction rate of the tea polysaccharide is high, and the tea polysaccharide in the obtained tea fuzz has obvious anti-oxidation and anti-tumor activity at the same time, especially has relatively strong cancer cell proliferation inhibition activity on a human hepatoma cell line (HePG2), a human cervical cancer cell (HeLa) and a human promyelocytic leukemia cell (HL60), and has a relatively good application prospect in the fields of foods, skin care and medicines.

Description

Technical Field

[0001] The invention relates to the technical field of natural product extraction and preparation, and in particular to a method for separating and extracting tea hairs, tea polysaccharides and applications thereof. Background Art

[0002] The fuzz in tea leaves is also called trichome. It is mainly attached to the lower epidermis of young tea buds and leaves. It is an important basis for the classification of tea varieties and has an important impact on the quality of finished tea. Whether it is white tea, green tea, black tea or dark tea, finished tea with trichome is considered to be a symbol of high quality. Generally speaking, the tenderer the fresh leaves are, the more trichome there is. The trichome not only has a significant impact on the appearance of the tea leaves, but also after a series of complex processing steps, the substances contained in the trichome will also dissolve in the tea soup when brewing tea, which not only enhances the taste of the tea soup, but also makes the tea soup have a "white hair full of" phenomenon, which makes people feel very charming. Therefore, whether the finished tea has tea trichome is one of the main characteristics for evaluating the quality of Baihao Yinzhen.

[0003] At present, scholars at home and abroad mainly study various nutritional components in tea, such as tea polysaccharides, tea polyphenols, etc. However, there are few studies on the content of effective ingredients in tea hair. Tea polysaccharides are easily soluble in water, and water extraction has the advantages of low price, non-toxicity and safety. Therefore, it is of great significance to develop a separation and extraction method for tea hair, optimize the extraction process and purification method of tea polysaccharides in tea hair, so as to improve the extraction rate and purity of tea polysaccharides in tea hair, and further study its biological activity. Summary of the invention

[0004] In view of this, the present invention provides a method for separating and extracting tea hairs, tea polysaccharides and applications thereof to solve the above problems.

[0005] The technical solution of the present invention is achieved in this way:

[0006] A method for separating and extracting tea hairs comprises the following steps:

[0007] S1. Pre-freezing: taking fresh tea leaves and quick-freezing them with liquid nitrogen to obtain pre-frozen tea samples;

[0008] S2, separation: crush the pre-frozen tea sample, sieve it, and collect the tea leaves;

[0009] S3. Extraction of tea polysaccharides from tea fuzz: soak the tea fuzz in salt water for 20-30 minutes, extract with hot water, filter, precipitate with alcohol, centrifuge, separate the precipitate, deproteinize the precipitate, dialyze and freeze-dry to obtain the tea polysaccharides in the tea fuzz; the hot water extraction conditions include: temperature control at 80-95°C, water-to-solid ratio of 20-30:1 (mL / g), and extraction time of 2-3h.

[0010] Preferably, in step S1, the brine concentration is 2.5-4 wt % and the temperature is 35-40° C.

[0011] Preferably, the fresh tea leaves in step S1 are one bud and one leaf of Rucheng Baimao tea that have just begun to unfold.

[0012] Rucheng White Tea (Camellia sinensis var assamica cv. Rucheng) is one of the four rare wild tea varieties in Hunan Province. It has large leaves, many hairs, rich contents, wide adaptability, and excellent germplasm resources. It is a rare and precious large-leafed, hairy wild tea in China. It is distributed in the mountains around Jiulong River in Rucheng County, Chenzhou City. The unique growing environment has made Rucheng White Tea unique in quality.

[0013] Preferably, the liquid nitrogen quick freezing time in step S1 is 8 to 12 seconds.

[0014] Preferably, the sieving in step S2 is specifically sieving through standard sieves of 40 and 60 meshes in sequence.

[0015] Preferably, the alcohol precipitation in step S2 is specifically performed by adding 4 to 6 volumes of 75 to 95 vol% ethanol to the filtrate and leaving the mixture to stand at 2 to 5° C. for 12 to 24 hours.

[0016] Preferably, the precipitate in step S3 is deproteinized using a sevage deproteinization reagent.

[0017] Preferably, the dialysis in step S3 uses a dialysis bag with a molecular weight cut-off of 4000Da.

[0018] The present invention also provides tea polysaccharide, which is extracted by the above-mentioned tea hair separation and extraction method.

[0019] The present invention also provides the use of the tea polysaccharide as an antioxidant in food, medicine and skin care products.

[0020] The present invention also provides the use of the tea polysaccharide in preparing a drug for inhibiting human liver cancer cell line HePG2, human cervical cancer cell HeLa and / or human promyelocytic leukemia cell HL60.

[0021] Compared with the prior art, the present invention has the following beneficial effects:

[0022] The present invention provides a method for separating and extracting tea hairs. First, the tea leaves are pretreated by quick-freezing with liquid nitrogen, which can quickly reduce the temperature and cause the cell structure to break, thereby facilitating the separation of the tea hairs and the tea body. The collected and separated tea hairs are treated with salt water of appropriate concentration and temperature to enhance the osmotic effect, which helps to activate tea polysaccharides, facilitates the release from the tea hairs, and improves the extraction efficiency. The parameters of hot water extraction are adjusted to further improve the extraction rate of tea polysaccharides. In addition, experiments have proved that the tea polysaccharides in the obtained tea hairs have obvious antioxidant and anti-tumor activities at the same time, especially have strong inhibitory activity on cancer cell proliferation against human liver cancer cell line (HePG2), human cervical cancer cell (HeLa) and human promyelocytic leukemia cell (HL60), and have good application prospects in the fields of food, skin care and medicine. DETAILED DESCRIPTION

[0023] In order to better understand the technical content of the present invention, specific embodiments are provided below to further illustrate the present invention.

[0024] Unless otherwise specified, the experimental methods used in the embodiments of the present invention are all conventional methods.

[0025] Unless otherwise specified, the materials, reagents, etc. used in the embodiments of the present invention can be obtained from commercial sources.

[0026] Example 1

[0027] A method for separating and extracting tea hairs comprises the following steps:

[0028] S1. Pre-freezing: Take one bud and one leaf of Rucheng Baimao tea, and quickly freeze them in liquid nitrogen for 10 seconds to obtain a pre-frozen tea sample;

[0029] S2, separation: the tea sample is placed in a grinder and crushed, and then passed through a 40-mesh and 60-mesh standard sieve in turn, and the tea hairs are gathered on the 60-mesh sieve, and the tea hairs are collected;

[0030] S3. Extraction of tea polysaccharides from tea hair: 10 g of tea hair was soaked in 3.5 wt % saline at 35°C for 30 min, taken out and dried, and then extracted with hot water at 90°C, with a water-to-solid ratio of 25:1 (mL / g). The extraction time was 2.5 h, filtered, 5 times the volume of 75 vol % ethanol was added to the filtrate, and the filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged, and the supernatant was filtered under reduced pressure, and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0031] Example 2

[0032] A method for separating and extracting tea hairs comprises the following steps:

[0033] S1. Pre-freezing: Take one bud and one leaf of Rucheng Baimao tea, and quickly freeze them in liquid nitrogen for 8 seconds to obtain a pre-frozen tea sample;

[0034] S2, separation: the tea sample is placed in a grinder and crushed, and then passed through a 40-mesh and 60-mesh standard sieve in turn, and the tea hairs are gathered on the 60-mesh sieve, and the tea hairs are collected;

[0035] S3. Extraction of tea polysaccharides from tea hair: 10 g tea hair was soaked in 2.5 wt % saline at 40°C for 20 min, taken out and dried, and then extracted with hot water at 80°C, with a water-to-solid ratio of 20:1 (mL / g). The extraction time was 3 h, filtered, 5 times the volume of 95 vol % ethanol was added to the filtrate, and the filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged, and the supernatant was filtered under reduced pressure, and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0036] Example 3

[0037] A method for separating and extracting tea hairs comprises the following steps:

[0038] S1. Pre-freezing: Take one bud and one leaf of Rucheng Baimao tea, and quickly freeze them in liquid nitrogen for 12 seconds to obtain a pre-frozen tea sample;

[0039] S2, separation: the tea sample is placed in a grinder and crushed, and then passed through a 40-mesh and 60-mesh standard sieve in turn, and the tea hairs are gathered on the 60-mesh sieve, and the tea hairs are collected;

[0040] S3. Extraction of tea polysaccharides from tea hairs: 10 g of tea hairs were soaked in 4 wt % saline at 40°C for 20 min, taken out and dried, and then extracted with hot water at 95°C, with a water-to-solid ratio of 30:1 (mL / g). The extraction time was 2 h, filtered, 5 times the volume of 75 vol% ethanol was added to the filtrate, and the filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged, and the supernatant was filtered under reduced pressure, and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇=5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0041] Comparative Example 1

[0042] The difference between this comparative example and Example 1 is that the liquid nitrogen quick-freezing treatment is not performed in the method for separating and extracting tea hairs, and one bud and one leaf of Rucheng white tea, which are just beginning to unfold, are directly placed in a grinder for crushing, and the remaining steps and parameters are the same as those in Example 1.

[0043] That is, a method for separating and extracting tea hairs, comprising the following steps:

[0044] S1. Take one bud and one leaf of Rucheng Baimao tea, put it into a grinder, grind it, and then pass it through 40 and 60 mesh standard sieves in turn. The tea hairs gather on the 60 mesh sieve and collect the tea hairs;

[0045] S2. Extraction of tea polysaccharides from tea fuzz: 10 g tea fuzz was soaked in 3.5 wt % saline at 35°C for 30 min, taken out and dried, and then extracted with hot water at 90°C, with a water-to-solid ratio of 25:1 (mL / g). The extraction time was 2.5 h, filtered, 5 times the volume of 75 vol % ethanol was added to the filtrate, and the filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged, and the supernatant was filtered under reduced pressure, and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0046] Comparative Example 2

[0047] The difference between this comparative example and Example 1 is that in the method for separating and extracting tea fuzz, step S1 is to take one bud and one leaf of Rucheng Baimao tea, which is just beginning to unfold, and quickly freeze it with liquid nitrogen for 18 seconds to obtain a pre-frozen tea sample, and the remaining steps and parameters are the same as those in Example 1.

[0048] That is, a method for separating and extracting tea hairs, comprising the following steps:

[0049] S1. Pre-freezing: Take one bud and one leaf of Rucheng Baimao tea, and quickly freeze them in liquid nitrogen for 18 seconds to obtain a pre-frozen tea sample;

[0050] S2, separation: the tea sample is placed in a grinder and crushed, and then passed through a 40-mesh and 60-mesh standard sieve in turn, and the tea hairs are gathered on the 60-mesh sieve, and the tea hairs are collected;

[0051] S3. Extraction of tea polysaccharides from tea hair: 10 g of tea hair was soaked in 3.5 wt % saline at 35°C for 30 min, taken out and dried, and then extracted with hot water at 90°C, with a water-to-solid ratio of 25:1 (mL / g). The extraction time was 2.5 h, filtered, 5 times the volume of 75 vol % ethanol was added to the filtrate, and the filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged, and the supernatant was filtered under reduced pressure, and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0052] Comparative Example 3

[0053] The difference between this comparative example and Example 1 is that in the method for separating and extracting tea hairs, step S3 does not use salt water immersion treatment, and the remaining steps and parameters are the same as those in Example 1.

[0054] That is, a method for separating and extracting tea hairs, comprising the following steps:

[0055] S1. Pre-freezing: Take one bud and one leaf of Rucheng Baimao tea, and quickly freeze them in liquid nitrogen for 10 seconds to obtain a pre-frozen tea sample;

[0056] S2, separation: the tea sample is placed in a grinder and crushed, and then passed through a 40-mesh and 60-mesh standard sieve in turn, and the tea hairs are gathered on the 60-mesh sieve, and the tea hairs are collected;

[0057] S3. Extraction of tea polysaccharides from tea fuzz: 10 g tea fuzz was extracted with hot water at 90°C, with a water-to-solid ratio of 25:1 (mL / g) for 2.5 h. The filtrate was filtered and 5 times the volume of 75 vol% ethanol was added to the filtrate. The filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged. The supernatant was filtered under reduced pressure and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0058] Comparative Example 4

[0059] The difference between this comparative example and Example 1 is that in the method for separating and extracting tea hairs, the salt water concentration in step S3 is 8 wt % and the temperature is 35° C., and the remaining steps and parameters are the same as those in Example 1.

[0060] That is, a method for separating and extracting tea hairs, comprising the following steps:

[0061] S1. Pre-freezing: Take one bud and one leaf of Rucheng Baimao tea, and quickly freeze them in liquid nitrogen for 10 seconds to obtain a pre-frozen tea sample;

[0062] S2, separation: the tea sample is placed in a grinder and crushed, and then passed through a 40-mesh and 60-mesh standard sieve in turn, and the tea hairs are gathered on the 60-mesh sieve, and the tea hairs are collected;

[0063] S3. Extraction of tea polysaccharides from tea fuzz: 10 g tea fuzz was soaked in 8 wt % saline at 35°C for 30 min, taken out and dried, and then extracted with hot water at 90°C, with a water-to-solid ratio of 25:1 (mL / g). The extraction time was 2.5 h, filtered, 5 times the volume of 75 vol% ethanol was added to the filtrate, and the filtrate was allowed to stand at 5°C for 24 h, centrifuged, and the precipitate was separated. The precipitate was re-dissolved and centrifuged, and the supernatant was filtered under reduced pressure, and then 1 / 5 volume of sevage reagent (V 氯仿 :V 正丁醇 =5:1) to perform protein removal operation, repeat several times until there is no protein layer, concentrate under reduced pressure to remove sevage reagent, place the protein-removed solution in a dialysis bag with a molecular weight cutoff of 4000Da for dialysis, and freeze-dry the dialysate to obtain tea polysaccharides in tea hairs.

[0064] Test Example 1 Extraction rate of tea polysaccharides from tea hairs

[0065] The extraction rates of tea polysaccharides from tea leaves of Examples 1 to 3 and Comparative Examples 1 to 4 were statistically analyzed, and the results are shown in Table 1.

[0066] Table 1 Extraction rate of tea polysaccharides from tea hairs

[0067] Extraction rate of tea polysaccharides from tea hairs (%) Example 1 12.3 Example 2 12.5 Example 3 12.4 Comparative Example 1 7.2 Comparative Example 2 8.7 Comparative Example 3 8.8 Comparative Example 4 10.1

[0068] As can be seen from the results in Table 1, the extraction rate of tea polysaccharides obtained by the separation and extraction methods of tea fuzz provided in Examples 1-3 of the present invention is significantly higher than that of the comparative example. Using liquid nitrogen to quickly freeze tea leaves can quickly reduce the temperature, causing the cell structure to break, thereby facilitating the separation of tea fuzz and the tea body. At the same time, it makes it easier to release the effective ingredients in the tea fuzz, thereby improving the extraction efficiency. In addition, the quick freezing process can reduce oxidation and maintain the activity of tea polysaccharides. Salt water of appropriate concentration and temperature can enhance the osmotic effect, help activate tea polysaccharides, facilitate release from tea fuzz, and improve extraction efficiency.

[0069] Test Example 2 DPPH free radical scavenging activity experiment

[0070] The tea polysaccharide and ascorbic acid obtained in Example 1 were respectively prepared into aqueous solutions of 0.1, 0.2, 0.5, 0.8, 1, and 1.5 mg / mL, and DPPH was dissolved in anhydrous ethanol under light-proof conditions to prepare a 5% DPPH ethanol solution. 2 mL of tea polysaccharide solutions of different concentrations were taken, and mixed evenly with 2 mL of 5% DPPH solution, and allowed to stand for 30 minutes in a dark place, and the absorbance was measured at 517 nm, with anhydrous ethanol as a blank control, deionized water as a negative control, and ascorbic acid as a positive control, and then calculated according to the following formula: DPPH free radical scavenging rate (%) = [1-(A-A0) / A1]×100; wherein A is the absorbance of the mixture of tea polysaccharide solution and DPPH solution, A0 is the absorbance of the mixture of tea polysaccharide solution and anhydrous ethanol (with the same volume as DPPH solution), and A1 is the absorbance of the mixture of deionized water and DPPH solution. The results are shown in Table 2. With the increase of the concentration of tea polysaccharide solution, the DPPH free radical scavenging rate gradually increased, indicating that the tea polysaccharides extracted from the fuzz of Rucheng white tea showed significant antioxidant capacity in vitro and can be used as antioxidants in food, medicine and skin care products.

[0071] Table 2 Antioxidant activity test of tea polysaccharides

[0072]

[0073] Test Example 3 Antitumor Activity Experiment

[0074] The anti-tumor activity of the tea polysaccharides prepared in Example 1, Comparative Example 1 and Comparative Example 3 of the present invention on human liver cancer cell line (HePG2), human gastric cancer cell line (SGC-7901), human cervical cancer cell line (HeLa), human lung cancer cell line (A-549) and human promyelocytic leukemia cell line (HL60) was tested by MTT method.

[0075] The experimental method is as follows:

[0076] (1) Cell subculture: The test cells stored in a liquid nitrogen deep freezer were thawed and revived in a 37°C water bath, and the cell lines were cultured in RPMI-1640 medium (100U / mL penicillin / streptomycin, 300mg / L-Glu) containing 10% fetal bovine serum in a 5% CO2 incubator at 37°C. The cells were grown adherently, and when the confluence rate reached 75-90%, the cells were digested with trypsin-EDTA digestion solution and collected for subsequent experiments.

[0077] (2) Test substance intervention treatment: 40 mg / mL tea polysaccharides were mixed with RPMI-1640 culture medium to a final concentration of 0, 31.25, 62.5, 125, 250, 500, and 1000 μg / mL, with 0 μg / mL as the control group. Then, 3000 to 5000 cells were inoculated into a 96-well plate, with 3 replicate wells for each group. The plates were gently tapped to remove bubbles and mixed thoroughly.

[0078] (3) MTT method to determine cell activity: After the cells were treated with tea polysaccharides for 48 hours, the original culture medium in the wells was discarded, and the fresh culture medium was mixed with 10μL MTT (5mg / mL) in the dark and then added to each well. The culture was continued for 4 hours under the original conditions and then a color reaction was performed. The original culture medium was discarded, and 100μL DMSO was added to each well. The cells were reacted at 37°C and 5% CO2 for 10 minutes and then oscillated in the dark for 10 minutes until the crystals were fully dissolved. The OD value was then measured at a wavelength of 490nm, and the cell proliferation rate was calculated. The cell proliferation rate (%) = OD value of the test group / OD value of the control group × 100. The half-inhibitory concentration (IC50) of the corresponding tea polysaccharide on the cells was analyzed by cell inhibition rate using GraphPad Prism 9.0. 50 ).

[0079] The results are shown in Table 3 (all results are expressed as mean ± SD, n = 3 for each group).

[0080] Table 3 Inhibitory activity of tea polysaccharides on tumor cell proliferation

[0081]

[0082] The results showed that the tea polysaccharides extracted from the fuzz of Rucheng Baimao tea showed certain cytotoxicity to the tested HePG2, SGC-7901, HeLa, A-549 and HL60 cell lines, especially to human liver cancer cell line (HePG2), human cervical cancer cell line (HeLa) and human promyelocytic leukemia cell line (HL60). Liquid nitrogen quick freezing and salt water immersion pretreatment of tea fuzz can enhance the anti-tumor effect of the extracted tea polysaccharides.

[0083] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for separating and extracting tea hair, characterized in that: The following steps are involved: S1. Pre-freezing: taking fresh tea leaves and quick-freezing them with liquid nitrogen to obtain pre-frozen tea samples; S2, separation: crush the pre-frozen tea sample, sieve it, and collect the tea leaves; S3. Extraction of tea polysaccharides from tea fuzz: soak the tea fuzz in salt water for 20 to 30 minutes, extract with hot water, filter, precipitate with alcohol, centrifuge, separate the precipitate, deproteinize the precipitate, dialyze and freeze-dry to obtain the tea polysaccharides in the tea fuzz; the hot water extraction conditions include: temperature control at 80 to 95°C, water-to-solid ratio of 20 to 30:1 (mL / g), and extraction time of 2 to 3 hours.

2. The method for separating and extracting tea hair according to claim 1, characterized in that: In step S1, the brine concentration is 2.5-4 wt % and the temperature is 35-40° C.

3. The method for separating and extracting tea hair according to claim 1, characterized in that: The fresh tea leaves in step S1 are one bud and one leaf of Rucheng Baimao tea, which are just beginning to unfold.

4. The method for separating and extracting tea hair according to claim 1, characterized in that: The liquid nitrogen quick freezing time in step S1 is 8 to 12 seconds.

5. The method for separating and extracting tea hair according to claim 1, characterized in that: The sieving in step S2 specifically includes sieving through standard sieves of 40 and 60 meshes in sequence.

6. The method for separating and extracting tea hair according to claim 1, characterized in that: The alcohol precipitation in step S2 is specifically performed by adding 4 to 6 volumes of 75 to 95 vol% ethanol to the filtrate and leaving it to stand at 2 to 5° C. for 12 to 24 hours.

7. The method for separating and extracting tea hair according to claim 1, characterized in that: In step S3, a dialysis bag with a molecular weight cut-off of 4000 Da is used for dialysis.

8. A tea polysaccharide, characterized in that: The tea hairs are obtained by extraction using the tea hair separation and extraction method according to any one of claims 1 to 7.

9. Use of the tea polysaccharide according to claim 8 as an antioxidant in food, medicine and skin care products.

10. Use of the tea polysaccharide according to claim 8 in the preparation of inhibitory drugs for human liver cancer cell line HePG2, human cervical cancer cell line HeLa and / or human promyelocytic leukemia cell line HL60.

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