Environment simulation device applied to biopesticide strain cultivation

Abstract

The invention belongs to the technical field of cultivation experiments, and particularly relates to an environment simulation device applied to biopesticide strain cultivation, which comprises a box body with an opening in the front side, a plurality of partition plates are uniformly and fixedly connected to the inner wall of the box body, and a plurality of temperature control boxes are fixedly connected to the rear side wall of the box body between the partition plates. A temperature control assembly is arranged in the temperature control box; a plurality of placement plates are arranged between the partition plates, the placement plate on the lowermost side is slidably connected with the partition plates, shear type lifting assemblies are arranged between the placement plates, two placement grooves are formed in the top surfaces of the placement plates, and magnetic stirring assemblies are arranged at the positions, located on the lower sides of the placement grooves, of the bottom surfaces of the placement plates. A plurality of culture dishes can be placed under different environment simulation conditions, acquisition of different experimental data is facilitated, and the culture effect is improved.

Description

Technical Field

[0001] The invention belongs to the technical field of cultivation experiments, and in particular relates to an environment simulation device used for cultivating biological pesticide strains. Background Art

[0002] Biopesticides are pesticides that use natural substances such as microorganisms, plant extracts, and insect hormones to control pests and diseases. In silkworm farming, the selection of suitable biopesticides needs to consider safety for silkworms, control effects on pests and diseases, and whether they are suitable for use in closed culture dishes. Common biopesticide cultivation strains include Bacillus thuringiensis and Metarhizium anisopliae.

[0003] However, in order to ensure the stability of the bacteria during the culture process and achieve the desired effect of pesticides, it is necessary to regularly use a microscope to observe the growth of the bacteria in different culture environments to ensure their normal growth and reproduction; but in the existing culture process of the bacteria, the culture dishes are placed in an incubator to control the temperature and humidity. Although the purpose of control can be achieved, only a small number of culture dishes can be placed in a single incubator, and the culture conditions of the culture dishes in the same incubator are the same. It is not convenient to place multiple culture dishes under different environmental conditions for cultivation, and it is impossible to effectively simulate and cultivate better bacteria, which affects the subsequent promotion and production and improvement of experimental data. Summary of the invention

[0004] The purpose of the present invention is to provide an environmental simulation device for the cultivation of biopesticide strains, which can place multiple culture dishes under different environmental simulation conditions, thereby facilitating the acquisition of different experimental data and improving the cultivation effect.

[0005] The technical solution adopted by the present invention is as follows:

[0006] An environmental simulation device for cultivating biological pesticide strains, comprising a box body with an opening at the front side, a plurality of partitions being evenly fixedly connected to the inner wall of the box body, a plurality of temperature control boxes being fixedly connected to the rear side wall of the box body between the partitions, and a temperature control component being arranged in the temperature control box;

[0007] A plurality of placement plates are arranged between the partitions, the lowermost placement plate is slidably connected to the partition, a scissor-type lifting assembly is arranged between the placement plates, two placement grooves are provided on the top surface of the placement plates, and a magnetic stirring assembly is arranged on the bottom surface of the placement plates below the placement grooves.

[0008] The temperature control component comprises a hot air blower installed in the inner cavity of the temperature control box, a guide pipe is installed on the hot air blower, and a side wall of the guide pipe is equipped with a plurality of adjustable nozzles, and the adjustable nozzles extend into the box body.

[0009] The scissor-type lifting assembly includes slide rails, which are respectively fixedly connected to the top surface of the lower placement plate and the bottom surface of the upper placement plate. The side walls of the slide rails are slidably connected with sliders. Cross-linking rods are arranged between the placement plates. The intersection of the cross-linking rods is a rotational connection. The front ends of the cross-linking rods are respectively hinged to the placement plates on both sides, and the rear ends of the cross-linking rods are respectively hinged to the sliders on both sides. The side walls of the lower slide rails are respectively provided with two limit members.

[0010] The limiting member comprises a circular groove formed on the top surface of the slide rail, a spring is fixedly connected to the bottom surface of the circular groove, an arc block is fixedly connected to the top of the spring, an arc groove is formed on the inner side wall of the slider, and the arc block is matched with the arc groove.

[0011] The left and right side walls of the placement plate are respectively fixedly connected to limit blocks, the box body and the side walls of the partition are respectively provided with limit grooves, the limit grooves penetrate the front side walls of the box body, and the top surface of the placement plate near the edge is fixedly connected with a blocking bar.

[0012] The bottom surface of the limiting block is equipped with a plurality of movable wheels, and the movable wheels abut against the bottom surface of the limiting groove.

[0013] The side walls opposite to the limiting block are both threadedly connected with studs, and one of the stud side walls is threadedly connected with a threaded sleeve.

[0014] The magnetic stirring assembly includes a motor fixedly connected to the bottom surface of a placement plate, a cavity is opened inside the placement plate at the lower side of the placement groove, a magnetic rod is arranged in the cavity, and an output end of the motor extends into the cavity and is fixedly connected to the magnetic rod.

[0015] The front side wall of the box body is equipped with a sealing door, and the sealing door is equipped with an observation window.

[0016] The technical effects achieved by the present invention are:

[0017] The environmental simulation device for cultivating biological pesticide strains of the present invention can place culture dishes under different environmental simulation conditions according to the cultivation conditions, so that the strains can grow under different environmental conditions, which is beneficial to the development of experiments and the improvement of subsequent experimental data, and further improves the application of strains in pesticides.

[0018] The environmental simulation device for cultivating biopesticide strains of the present invention can place multiple layers of culture dishes in the same simulation space through the cooperation between multiple placement plates, scissor-type lifting components and magnetic stirring components, and each culture dish is equipped with an independent magnetic stirring structure, which is convenient for stirring according to experimental requirements during the strain cultivation process, thereby achieving the purpose of multifunctional cultivation. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1is a stereogram of an embodiment of the present invention;

[0020] Figure 2 It is a partial structural schematic diagram of an embodiment of the present invention;

[0021] Figure 3 is a rear view structural schematic diagram of an embodiment of the present invention;

[0022] Figure 4 is a schematic diagram of the distribution structure of the placement plate according to an embodiment of the present invention;

[0023] Figure 5 Embodiment of the present invention Figure 4 A schematic cross-sectional structure diagram of ;

[0024] Figure 6 is a schematic structural diagram of a scissor lift assembly according to an embodiment of the present invention;

[0025] Figure 7 Embodiment of the present invention Figure 6 A in the enlarged view;

[0026] Figure 8 Embodiment of the present invention Figure 2 Enlarged view of point B

[0027] Fig. 9 Embodiment of the present invention Figure 4 Enlarged view of point C in the figure.

[0028] In the attached drawings, the list of components represented by each reference numeral is as follows: 1. Box body; 2. Partition; 3. Temperature control box; 4. Hot air blower; 5. Guide pipe; 6. Adjustable nozzle; 7. Placement plate; 8. Placement slot; 9. Motor; 10. Magnetic rod; 11. Slide rail; 12. Slider; 13. Cross connecting rod; 14. Circular groove; 15. Spring; 16. Arc block; 17. Arc groove; 18. Limit block; 19. Limit groove; 20. Baffle; 21. Stud; 22. Screw sleeve; 23. Movable wheel; 24. Sealing door. DETAILED DESCRIPTION

[0029] In order to make the purpose and advantages of the present invention more clearly understood, the present invention is specifically described below in conjunction with embodiments. It should be understood that the following text is only used to describe one or several specific embodiments of the present invention, and does not strictly limit the scope of protection of the specific claims of the present invention.

[0030] like Figure 1-Figure 9 As shown, an environmental simulation device for cultivating biological pesticide strains includes a box body 1 with an opening at the front side, a plurality of partitions 2 are evenly and fixedly connected to the inner wall of the box body 1, a plurality of temperature control boxes 3 are fixedly connected to the rear side wall of the box body 1 between the partitions 2, and a temperature control component is arranged in the temperature control box 3;

[0031] Multiple placement plates 7 are arranged between the partitions 2, the lowest placement plate 7 is slidably connected to the partition 2, scissor-type lifting components are arranged between the placement plates 7, two placement grooves 8 are opened on the top surface of the placement plates 7, and a magnetic stirring component is arranged on the bottom surface of the placement plates 7 below the placement grooves 8.

[0032] like Figure 2 and Figure 3 As shown, the temperature control component includes a hot air blower 4 installed in the inner cavity of the temperature control box 3, a guide tube 5 is installed on the hot air blower 4, and a side wall of the guide tube 5 is equipped with a plurality of adjustable nozzles 6, which extend into the interior of the box 1.

[0033] Specifically, in addition to the temperature control component for controlling the internal temperature of the box 1, the following functions can also be set according to experimental requirements:

[0034] Humidity sensor: used to monitor the humidity level in the box. Common types include capacitive humidity sensors and resistive humidity sensors;

[0035] Humidifier: used to increase the humidity in the box. Common humidifiers include steam humidifiers and water tank humidifiers;

[0036] Dehumidifier: used to reduce the humidity in the box. Common dehumidifiers include condensing dehumidifiers and desiccant dehumidifiers;

[0037] Fan: used to circulate the air in the box to ensure uniform distribution of temperature and humidity;

[0038] Air filter: used to filter the air entering the box to prevent dust and microbial contamination;

[0039] LED lights or fluorescent lights: provide the required light to ensure photosynthesis and microbial growth;

[0040] Over-temperature protection: When the temperature exceeds the set value, the heater is automatically cut off or the cooling system is started to prevent the temperature from being too high;

[0041] Low humidity alarm: When the humidity is lower than the set value, an alarm will sound and the humidifier will start;

[0042] CO2 control system: used to control the carbon dioxide concentration in the box, especially suitable for the cultivation of plants and specific microorganisms.

[0043] The above facilities are all mature technologies in the existing market, so they will not be described in detail in this solution. They are mainly used to control the temperature and humidity inside the box 1 and other functions to simulate the natural environment. By simulating the environmental state to adapt to the growth state of the culture in different environments, it is beneficial to extract better culture data and provide data support for subsequent improvement experiments.

[0044] According to the cultivation conditions, the culture dishes can be placed in different environmental simulation conditions, so that the bacteria can grow in different environmental conditions, which is conducive to the development of experiments and the improvement of subsequent experimental data, and further improves the application of lecithin-cultivated bacteria in pesticides.

[0045] like Figure 4-Figure 6 As shown, the scissor-type lifting assembly includes a slide rail 11, which is fixedly connected to the top surface of the lower placement plate 7 and the bottom surface of the upper placement plate 7 respectively. The side walls of the slide rail 11 are slidably connected with sliders 12. Cross links 13 are arranged between the placement plates 7. The intersection of the cross links 13 is a rotational connection. The front ends of the cross links 13 are hinged to the placement plates 7 on both sides respectively, and the rear ends of the cross links 13 are hinged to the sliders 12 on both sides respectively. The side walls of the lower slide rail 11 are provided with two limit members.

[0046] Among them, by setting up a multi-layer scissor lift assembly, the user can open a specified number of placement plates 7 according to the culture requirements for placing culture dishes, and the number of placement plates 7 and the number of placement slots 8 can be adjusted according to the experimental requirements, which is not limited in this solution. In addition, the scissor lift structure is stable and can provide reliable support in the vertical and horizontal directions. In this solution, manual operation is used as the driving method, and the limiter is used for locking. It has the characteristics of simple structure, easy operation, and no need for electric drive.

[0047] like Figure 6 and Figure 7 As shown, the limiter includes a circular groove 14 opened on the top surface of the slide rail 11, a spring 15 is fixedly connected to the bottom surface of the circular groove 14, an arc block 16 is fixedly connected to the top of the spring 15, an arc groove 17 is opened on the inner side wall of the slider 12, and the arc block 16 is adapted to the arc groove 17.

[0048] Specifically, when multiple placement plates 7 are in an overlapping state, the arc groove 17 on the slider 12 cooperates with the rear arc block 16. When the placement plate 7 needs to be opened, the slider 12 slides forward on the slide rail 11 during the rising process of the placement plate 7. When the slider 12 abuts against the front arc block 16, the placement plate 7 continues to be pulled, and the arc block 16 is pushed forward by the slider 12 to move into the circular groove 14. When the arc block 16 corresponds to the arc groove 17, the force of the spring 15 is used to drive the arc block 16 to be stuck in the arc groove 17, thereby realizing the limitation of the placement plate 7.

[0049] like Figure 8 As shown, the left and right side walls of the placement plate 7 are respectively fixedly connected to the limit blocks 18, and the side walls of the box body 1 and the partition 2 are respectively provided with limit grooves 19, which penetrate the front side wall of the box body 1, and the top surface of the placement plate 7 near the edge is fixedly connected with a stop bar 20. The bottom surface of the limit block 18 is equipped with a plurality of movable wheels 23, and the movable wheels 23 are against the bottom surface of the limit groove 19.

[0050] Among them, the limiting blocks 18 and the limiting grooves 19 can, on the one hand, limit the multiple placement plates 7, ensure the stability of the placement plates 7 when being pushed in or pulled out, and reduce the probability of the culture shaking; on the other hand, they can support the placement plates 7, increase the force points of the placement plates 7, and thus improve the flexibility of the placement plates 7; and by passing the limiting grooves 19 through the box body 1, the placement plates 7 can be removed as a whole and replaced with placement plates 7 of different numbers or layer heights; in addition, the movable wheels 23 can reduce the friction between the placement plates 7 and the box body 1 and the partition 2.

[0051] like Fig. 9 As shown, the side walls on the opposite side of the limiting block 18 are both threadedly connected with studs 21, and a threaded sleeve 22 is threadedly connected to the side wall of one of the studs 21.

[0052] Specifically, when multiple placement plates 7 need to be pulled out or pushed in synchronously, the screw sleeve 22 can be rotated so that the screw sleeve 22 is located between the studs 21 on both sides, and the two adjacent studs 21 are connected by the screw sleeve 22, so that the placement plates 7 in different chambers are connected as a whole. When one of the placement plates 7 is pulled out, the placement plates 7 at other positions are pulled out synchronously to facilitate the unified placement and removal of the culture dishes; in addition, the position of the limit groove 19 can be sealed by using the baffle 20 to prevent the temperature in the space on both sides of the partition 2 from circulating and affecting the accuracy of the cultivation conditions.

[0053] like Figure 5 As shown, the magnetic stirring assembly includes a motor 9 fixedly connected to the bottom surface of the placement plate 7, a cavity is provided inside the placement plate 7 at the lower side of the placement slot 8, a magnetic rod 10 is provided in the cavity, and the output end of the motor 9 extends into the cavity and is fixedly connected to the magnetic rod 10. The magnetic stirring assembly belongs to the existing mature technology, and in this solution it mainly plays the role of auxiliary stirring, and can be used according to actual needs. In addition, when the placement plates 7 are in an overlapping state, the upper motor 9 can be stored in the placement slot 8.

[0054] Through the coordination between multiple placement plates 7, scissor-type lifting components and magnetic stirring components, multiple layers of culture dishes can be placed in the same simulation space, and each culture dish is equipped with an independent magnetic stirring structure, which is convenient for stirring according to experimental requirements during the culture process of the strains, thereby achieving the purpose of multifunctional cultivation.

[0055] like Figure 1 As shown, the front side wall of the box body 1 is equipped with a sealing door 24, and an observation window is installed on the sealing door 24. The space between the partitions 2 can be sealed by setting the sealing door 24, and a plurality of observation windows are set on the sealing door 24 and correspond to each layer of culture dishes, so as to observe the culture status in time.

[0056] The working principle of the present invention is as follows: first, the sealing door 24 is opened, and the placement plate 7 is pulled outward, and then the upper placement plate 7 is opened upward according to the number of culture dishes, and the upper placement plate 7 is first pulled upward to change the cross link 13 from the folded state to the opened state, and at the same time, the slider 12 moves on the slide rail 11, and when the slider 12 moves to a suitable position, the arc block 16 is driven by the force of the spring 15 to be stuck in the arc groove 17, which is used to lock the slider 12, so as to achieve the function of fixing and limiting the placement plate 7, and then the culture dishes are placed in the placement groove 8 in sequence, and then the placement plate 7 is pushed into the box body 1;

[0057] After the culture dish is placed, the hot air blower 4 can be started as needed to inject hot air flows of different temperatures between the partitions 2 to simulate the effect of different temperatures in the environment. When the culture needs to be mixed and stirred, it can be placed in the culture dish; the magnetic rod, and the motor 9 is started to drive the magnetic rod 10 to rotate. The magnetic rod 10 is attracted to the magnetic rod and drives it to rotate, so as to mix the culture and achieve the purpose of efficient cultivation.

[0058] The above is only a preferred embodiment of the present invention. It should be noted that, for those skilled in the art, several improvements and modifications can be made without departing from the principles of the present invention, and these improvements and modifications should also be considered as the protection scope of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention shall be implemented according to the conventional means in the art unless otherwise specified and limited.

Claims

1. An environmental simulation device for cultivating biological pesticide strains, characterized by: It comprises a box body (1) with an opening at the front side, a plurality of partitions (2) being evenly and fixedly connected to the inner wall of the box body (1), a plurality of temperature control boxes (3) being fixedly connected to the rear side wall of the box body (1) between the partitions (2), and a temperature control component being arranged in the temperature control box (3); A plurality of placement plates (7) are arranged between the partitions (2), the lowermost placement plate (7) is slidably connected to the partition (2), a scissor-type lifting assembly is arranged between the placement plates (7), two placement grooves (8) are provided on the top surface of the placement plate (7), and a magnetic stirring assembly is arranged on the bottom surface of the placement plate (7) below the placement groove (8).

2. The environmental simulation device for cultivating biological pesticide strains according to claim 1, characterized in that: The temperature control component comprises a hot air blower (4) installed in the inner cavity of the temperature control box (3), a flow guide tube (5) is installed on the hot air blower (4), and a plurality of adjustable nozzles (6) are installed on the side wall of the flow guide tube (5), and the adjustable nozzles (6) extend into the interior of the box body (1).

3. The environmental simulation device for cultivating biological pesticide strains according to claim 1, characterized in that: The scissor lift assembly comprises a slide rail (11), wherein the slide rail (11) is fixedly connected to the top surface of the lower placement plate (7) and the bottom surface of the upper placement plate (7), and the side wall of the slide rail (11) is slidably connected with a slider (12). A cross link (13) is provided between the placement plates (7), and the intersection of the cross link (13) is rotatably connected. The front end of the cross link (13) is hinged to the placement plates (7) on both sides, and the rear end of the cross link (13) is hinged to the sliders (12) on both sides. The side wall of the lower slide rail (11) is provided with two limit members.

4. The environmental simulation device for cultivating biological pesticide strains according to claim 3 is characterized by: The limiting member comprises a circular groove (14) formed on the top surface of the slide rail (11); a spring (15) is fixedly connected to the bottom surface of the circular groove (14); an arc block (16) is fixedly connected to the top of the spring (15); an arc groove (17) is formed on the inner side wall of the slide block (12); and the arc block (16) is adapted to the arc groove (17).

5. The environmental simulation device for cultivating biological pesticide strains according to claim 1, characterized in that: The left and right side walls of the placement plate (7) are respectively fixedly connected to limit blocks (18); the side walls of the box body (1) and the partition plate (2) are respectively provided with limit grooves (19); the limit grooves (19) penetrate the front side walls of the box body (1); and the top surface of the placement plate (7) near the edge is fixedly connected to a stop bar (20).

6. The environmental simulation device for cultivating biological pesticide strains according to claim 5, characterized in that: The bottom surface of the limiting block (18) is equipped with a plurality of movable wheels (23), and the movable wheels (23) abut against the bottom surface of the limiting groove (19).

7. The environmental simulation device for cultivating biological pesticide strains according to claim 5, characterized in that: The side walls on the opposite side of the limit block (18) are both threadedly connected with studs (21), and a threaded sleeve (22) is threadedly connected to the side wall of one of the studs (21).

8. The environmental simulation device for cultivating biological pesticide strains according to claim 1, characterized in that: The magnetic stirring assembly comprises a motor (9) fixedly connected to the bottom surface of a placement plate (7); a cavity is provided inside the placement plate (7) at a position below the placement groove (8); a magnetic rod (10) is arranged in the cavity; an output end of the motor (9) extends into the cavity and is fixedly connected to the magnetic rod (10).

9. The environmental simulation device for cultivating biological pesticide strains according to claim 1, characterized in that: The front side wall of the box body (1) is equipped with a sealing door (24), and the sealing door (24) is equipped with an observation window.

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