A sample sampling device for preparing stem cells

Abstract

The present invention discloses a sample sampling device for preparing stem cells. The present invention relates to the field of anti-pollution technology and includes a frame, a pretreatment mechanism, and a sampling mechanism. The pretreatment mechanism includes an electric telescopic rod, a pressing member is fixedly installed on the telescopic end of the electric telescopic rod, a liquid storage tube is fixedly installed on the top of the pressing member, an opening and closing assembly is installed on the bottom of the liquid storage tube, an elastic telescopic sleeve is fixedly installed between the outer cylindrical surface of the liquid storage tube and the top of the inner cavity of the shell, the sampling mechanism includes a sampler and a conical guide sleeve, a hose is installed in the middle of the bottom of the sampler, and the end of the hose away from the sampler is connected to a feeding round head, a sealing ring is fixedly installed at the feed inlet at the top of the feeding round head, and a lifting assembly is installed at the bottom of the inner cavity of the shell near the feeding round head. The sample sampling device for preparing stem cells achieves an anti-pollution effect, reduces the contact between stem cells and air, is not easy to cause contamination, and is safe and reliable for sampling.

Description

Technical Field

[0001] The present invention relates to the technical field of sampling equipment, in particular to a sample sampling device for preparing stem cells. Background Art

[0002] Stem cells are a type of cell with the ability to self-replicate and multidirectionally differentiate. Under certain conditions, they can differentiate into a variety of functional cells and tissues, possessing biological functions such as immune regulation and regeneration and repair. Stem cell secretions are a variety of active substances secreted by stem cells after specialized culture, including proteins, cytokines, and peptides, which have therapeutic effects in anti-aging, beauty, and cell repair. Stem cell sampling plays a significant role in stem cell cultivation and research. When processing blood, stem cells need to be separated and sampled, which requires stem cell sampling equipment.

[0003] Currently, during the existing stem cell sampling process, the stem cells are exposed to the outside, causing the stem cells to come into direct contact with the air, which can easily cause contamination of the stem cells, thereby reducing the activity of the stem cells and affecting the sampling of the stem cells. Summary of the Invention

[0004] To achieve the above objectives, the present invention is implemented through the following technical solutions: A sample sampling device for preparing stem cells, comprising:

[0005] A frame having a supporting frame body, a shell installed on the top of the frame, and a feeding mechanism installed in the middle of the top of the shell;

[0006] a pre-treatment mechanism, the pre-treatment mechanism being used for stratifying blood, the pre-treatment mechanism being installed in the middle of the top of the inner cavity of the housing;

[0007] The pretreatment mechanism includes an electric telescopic rod, which is installed on the side of the top of the inner cavity of the shell. A pressing piece is fixedly installed on the telescopic end of the electric telescopic rod, and a liquid storage tube is fixedly installed on the top of the pressing piece. An opening and closing assembly is installed on the bottom end of the liquid storage tube. An elastic telescopic sleeve is fixedly installed between the outer circular surface of the liquid storage tube and the top of the inner cavity of the shell. A magnetic blocking ball is fixedly installed on the inclined surface of the inner cavity of the liquid storage tube and near the top. The telescopic end of the electric telescopic rod is contracted to drive the liquid storage tube to move upward, and the elastic telescopic sleeve is compressed. Combined with the top of the liquid storage tube being inside the elastic telescopic sleeve, it can be isolated from the outside air and is not easily contaminated by the outside air, thereby facilitating the survival of stem cells.

[0008] A sampling mechanism, which is used to collect stem cells and is installed at the bottom of the housing and close to the frame;

[0009] Wherein, the sampling mechanism includes a sampler and a conical guide sleeve, the sampler is installed at the side of the outer side of the shell, the sampler is installed near the frame, the conical guide sleeve is fixedly installed in the middle of the bottom of the shell, a hose is installed in the middle of the bottom of the sampler, the hose passes through the center of the conical guide sleeve and extends to the interior of the shell, the end of the hose away from the sampler is connected with a feeding round head, a sealing ring is fixedly installed at the feeding port on the top of the feeding round head, a lifting component is installed at the bottom of the inner cavity of the shell and near the feeding round head, a pushing force is applied to the feeding round head by the lifting component, so that the feeding round head moves upward, and room is left in combination with the hose, so that the feeding round head moves upward smoothly, and it is not easy to get stuck in the structure, and the upward moving feeding round head coincides with the bottom end of the liquid storage tube;

[0010] At the same time, the staff turns on the sampler and uses the suction generated by the sampler and the conveying of the hose to make the round head suck out the stem cells so that the sample can be collected.

[0011] Preferably, there are two electric telescopic rods, and the two electric telescopic rods are symmetrically installed along the liquid storage tube. The liquid storage tube passes through the center of the elastic telescopic sleeve, so that the top of the liquid storage tube can be isolated by the elastic telescopic sleeve.

[0012] After the mixed liquid material enters the liquid storage tube, the material can be left to stand to allow the mixed material to be stratified, the stem cells to be separated, and the material to be pre-treated, thereby facilitating the sampling of the stem cells.

[0013] Preferably, the opening and closing assembly includes a right-angle elastic piece and an annular positioning sleeve, the right-angle elastic piece is fixedly mounted on the inner wall of the liquid storage tube and close to the bottom position, the annular positioning sleeve is fixedly mounted on the bottom end of the liquid storage tube, and a ball is fixedly mounted on one end of the right-angle elastic piece away from the inner wall of the liquid storage tube.

[0014] Preferably, the right-angle elastic pieces are evenly distributed between the spherical surface outside the sphere and the inner wall of the liquid storage tube, and the annular positioning sleeve is installed directly below the liquid storage tube.

[0015] Preferably, the sealing ring is made of rubber, which is convenient for sealing the edge of the feed port at the top of the feeding round head. The lifting components are in two groups, and the two groups of lifting components are symmetrically installed along the feeding round head.

[0016] The push rod has a U-shaped bottom end, and the push rod has a U-shaped bottom end. The push rod has a U-shaped bottom end, and the push rod has a U-shaped bottom end. The push rod has a U-shaped bottom end, and the push rod has a U-shaped bottom end. The push rod has a U-shaped bottom end, and the push rod has a U-shaped bottom end. The liquid tube moves downward with the material, and applies pressing force to the end of the arched push rod away from the material-taking round head through the pressing piece. Under the support of the U-shaped bracket and the sliding installation through the arc hole, the material-taking round head is lifted up, and the return spring is stretched. The upward-moving material-taking round head is embedded in the annular positioning sleeve, and the material-taking round head applies an upward pushing force to the ball, so that the ball moves upward, and the right-angle elastic sheet is compressed, and the discharge hole at the bottom of the liquid storage tube is opened. Under the sealing of the sealing ring, it is convenient to take the stem cells at the bottom of the liquid storage tube through the material-taking round head.

[0017] Preferably, there are two arched push rods, and the two arched push rods are symmetrically installed along the material-taking round head. The reset spring is installed directly below the arched push rod to facilitate applying elastic tension to the arched push rod, thereby helping the arched push rod to reset.

[0018] Preferably, the feeding mechanism includes a square box and a discharge port, the edge of the bottom of the square box is fixed to the top of the shell by screws, the discharge port is opened at the bottom of the square box, and the position of the discharge port corresponds to the position of the magnetic sealing ball, a reagent feeder is installed at the side of the top of the square box, a right-angle tube is installed on the top of the square box and on the side away from the reagent feeder, a servo motor is installed in the middle of the top of the square box, a rotating roller is rotatably installed in the middle of the inner cavity of the square box, the top of the rotating roller is fixed to the output end of the servo motor through a coupling, a mixing blade is fixedly installed in the middle of the outer cylindrical surface of the rotating roller, and a flipping assembly is installed on the outer cylindrical surface of the rotating roller near the bottom. When the liquid storage tube moves upward, it can drive the magnetic sealing ball to move upward together, and by using the position of the magnetic sealing ball corresponding to the position of the discharge port, the magnetic sealing ball can be embedded in the inside of the discharge port, thereby sealing the discharge port, and it is not easy to leak material at will.

[0019] By extending the output end of the electric telescopic rod, the liquid storage tube can be driven to move downward, and the magnetic sealing ball can be driven to move downward, thereby separating the magnetic sealing ball from the discharge port, so that the discharge port is opened, and the mixed liquid material flows downward from the discharge port and is promptly connected to the liquid storage tube for subsequent processing.

[0020] The output end of the servo motor is rotated to drive the rotating roller to rotate, so that the mixing blade is driven to rotate together, and the rotating mixing blade can be used to mix the blood and reagent in the square box.

[0021] Preferably, the flipping assembly includes a supporting round rod, which is fixedly mounted on the outer circumference of the rotating roller and close to the bottom position, and a flipping plate is rotatably mounted on the outer circumference of the supporting round rod and close to the rotating roller, and a torque spring is fixedly mounted between one end of the supporting round rod away from the rotating roller and the side of the outer side of the flipping plate, and a magnetic ball is fixedly mounted on the outer side of the flipping plate and close to the bottom position, and the magnetic ball and the magnetic sealing ball have the same magnetic poles. When the rotating roller rotates, it drives the flipping assembly as a whole to rotate, and when the magnetic ball and the magnetic sealing ball are close to each other, the two generate a repulsive magnetic force, and under the support of the supporting round rod, the flipping plate is subjected to a reverse magnetic thrust to rotate counterclockwise to adjust the angle, and the torque spring is elastically compressed by the torque force;

[0022] As the flipping plate drives the magnetic ball to continue rotating, the magnetic ball moves away from the magnetic blocking ball, the reverse magnetic thrust disappears, and under the elastic force of the torque spring, the flipping plate rotates in the opposite direction. In this way, the blood and reagents in the square box can be flipped by the reciprocating swing of the flipping plate, so that the materials are fully integrated together, and the interaction between the structures is utilized to connect the structures together.

[0023] Preferably, the flipping plate is arc-shaped, there are two flipping plates, and the two flipping plates are installed symmetrically along the axis of the rotating roller, and the supporting round rod passes through the center of the torque spring.

[0024] The present invention provides a sample collection device for preparing stem cells. It has the following beneficial effects:

[0025] 1. The sample sampling device for preparing stem cells applies a pushing force to the material collection head by lifting the assembly, causing the material collection head to move upward. The flexible tube leaves room for smooth upward movement of the material collection head, making it less likely for the structure to become stuck. The upwardly moving material collection head aligns with the bottom end of the liquid storage tube. At the same time, the staff turns on the sampler to work, using the suction generated by the sampler and the conveyance of the flexible tube to allow the material collection head to suck out the stem cells, thereby allowing sample collection.

[0026] Second, the sample sampling device for preparing stem cells utilizes the telescopic end of the electric telescopic rod to retract, thereby driving the liquid storage tube to move upward. The elastic telescopic sleeve is compressed, and the top of the liquid storage tube is located inside the elastic telescopic sleeve, thereby isolating the liquid storage tube from the outside air and not easily contaminated by the outside air, thereby helping the survival of stem cells.

[0027] 3. The sample sampling device for preparing stem cells can drive the magnetic sealing ball to move upward when the liquid storage tube moves upward. By aligning the position of the magnetic sealing ball with the position of the discharge port, the magnetic sealing ball can be embedded in the interior of the discharge port, thereby sealing the discharge port and preventing random leakage.

[0028] 4. The sample sampling device for preparing stem cells, after the blood and reagent are mixed, can again extend the output end of the electric telescopic rod to drive the liquid storage tube downward, so that the magnetic sealing ball can be driven downward, thereby separating the magnetic sealing ball from the discharge port, so that the discharge port is opened, and the mixed liquid material flows downward from the discharge port and is promptly connected to the liquid storage tube for subsequent processing.

[0029] 5. The sample sampling device for preparing stem cells can allow the mixed liquid material to be left to stand after entering the liquid storage tube, so that the mixed material can be separated into layers, the stem cells can be separated, and the material can be pre-treated, thereby facilitating the sampling of stem cells.

[0030] 6. The sample sampling device for preparing stem cells uses a pressing member to apply pressing force to the end of the arched push rod away from the material collection round head, so that the material collection round head is pushed up, and the upward moving material collection round head is embedded in the annular positioning sleeve. The material collection round head applies an upward pushing force to the ball, causing the ball to move upward, and the right-angled elastic sheet is compressed, thereby opening the discharge hole at the bottom of the liquid storage tube. Under the sealing of the sealing ring, the stem cells at the bottom of the liquid storage tube can be easily collected through the material collection round head.

[0031] 7. The sample sampling device for preparing stem cells utilizes the output end of the servo motor to rotate, which can drive the rotating roller to rotate, so that the mixing blade is driven to rotate together, and the rotating mixing blade can be used to mix the blood and reagents in the square box.

[0032] 8. The sample sampling device for preparing stem cells uses the repulsive magnetic force generated by the proximity of a magnetic ball and a magnetic sealing ball, causing the flipping plate to rotate counterclockwise to adjust the angle under the reverse magnetic thrust. As the flipping plate drives the magnetic ball to continue rotating, the magnetic ball moves away from the magnetic sealing ball, the reverse magnetic thrust disappears, and the flipping plate rotates in the opposite direction under the elastic force of the torque spring. In this way, the flipping plate can swing back and forth to flip the blood and reagents in the square box, so that the materials are fully integrated together. BRIEF DESCRIPTION OF THE DRAWINGS

[0033] Figure 1 This is a schematic diagram of the overall structure of the sample sampling device for preparing stem cells according to the present invention;

[0034] Figure 2This is a schematic diagram of the cross-sectional structure of the sample sampling device for preparing stem cells according to the present invention;

[0035] Figure 3 Schematic diagram of the connection structure between the pretreatment mechanism and the housing of the present invention;

[0036] Figure 4 This is a schematic diagram of the overall structure of the opening and closing assembly of the present invention;

[0037] Figure 5 Schematic diagram of the connection structure between the sampling mechanism and the housing of the present invention;

[0038] Figure 6 This is a schematic diagram of the overall structure of the jacking assembly of the present invention;

[0039] Figure 7 Schematic diagram of the connection structure between the feeding mechanism and the housing of the present invention;

[0040] Figure 8 It is a schematic diagram of the overall structure of the flip assembly of the present invention.

[0041] Figure: 1, frame; 2, housing; 3, pre-treatment mechanism; 4, sampling mechanism; 5, feeding mechanism; 31, electric telescopic rod; 32, pressing member; 33, liquid storage tube; 34, opening and closing assembly; 35, elastic telescopic sleeve; 36, magnetic sealing ball; 341, right-angle elastic piece; 342, annular positioning sleeve; 343, round ball; 41, sampler; 42, conical guide sleeve; 43, hose; 44, round head for taking out material; 45 , sealing ring; 46, lifting assembly; 461, U-shaped bracket; 462, arched push rod; 463, arc-shaped hole; 464, reset spring; 51, square box; 52, reagent feeder; 53, right-angle tube; 54, servo motor; 55, rotating roller; 56, mixing blade; 57, flipping assembly; 58, discharge port; 571, supporting round rod; 572, flipping plate; 573, torque spring; 574, magnetic ball. DETAILED DESCRIPTION

[0042] The present invention will be described in further detail below with reference to the accompanying drawings and specific embodiments. The embodiments of the present invention are provided for purposes of illustration and description and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those skilled in the art. The embodiments are chosen and described to better illustrate the principles of the invention and its practical application, and to enable those skilled in the art to understand the invention and design various embodiments with various modifications suitable for specific applications.

[0043] The first embodiment, as Figure 1-Figure 4 As shown, the present invention provides a technical solution: a sample sampling device for preparing stem cells, comprising:

[0044] The frame 1 has a supporting frame body, a housing 2 is installed on the top of the frame 1, and a feeding mechanism 5 is installed in the middle of the top of the housing 2;

[0045] A pretreatment mechanism 3 is used to stratify blood and is installed in the middle of the top of the inner cavity of the housing 2;

[0046] The pretreatment mechanism 3 includes an electric telescopic rod 31, which is installed on the side of the top of the inner cavity of the shell 2. A pressing piece 32 is fixedly installed on the telescopic end of the electric telescopic rod 31, and a liquid storage tube 33 is fixedly installed on the top of the pressing piece 32. An opening and closing component 34 is installed on the bottom end of the liquid storage tube 33. An elastic telescopic sleeve 35 is fixedly installed between the outer circular surface of the liquid storage tube 33 and the top of the inner cavity of the shell 2. A magnetic blocking ball 36 is fixedly installed on the inclined surface of the inner cavity of the liquid storage tube 33 and near the top. When the staff turns on the electric telescopic rod 31 to work and uses the telescopic end of the electric telescopic rod 31 to contract, the liquid storage tube 33 can be driven to move upward, and the elastic telescopic sleeve 35 is compressed. Combined with the top of the liquid storage tube 33 being inside the elastic telescopic sleeve 35, it can be isolated from the outside air and is not easily contaminated by the outside air, thereby helping the survival of stem cells.

[0047] There are two electric telescopic rods 31 , and the two electric telescopic rods 31 are symmetrically installed along the liquid storage tube 33 . The liquid storage tube 33 passes through the center of the elastic telescopic sleeve 35 , so that the top of the liquid storage tube 33 can be isolated by the elastic telescopic sleeve 35 .

[0048] After the mixed liquid material enters the liquid storage tube 33, the material can be left to stand to allow the mixed material to be layered and the stem cells to be separated. The material can be pre-treated, thereby facilitating the sampling of the stem cells.

[0049] The opening and closing assembly 34 includes a right-angle elastic piece 341 and an annular positioning sleeve 342. The right-angle elastic piece 341 is fixedly installed on the inner wall of the liquid storage tube 33 and close to the bottom position. The annular positioning sleeve 342 is fixedly installed on the bottom end of the liquid storage tube 33. A ball 343 is fixedly installed on the end of the right-angle elastic piece 341 away from the inner wall of the liquid storage tube 33.

[0050] The right-angle elastic pieces 341 are evenly distributed between the spherical surface outside the sphere 343 and the inner wall of the liquid storage tube 33 , and the annular positioning sleeve 342 is installed directly below the liquid storage tube 33 .

[0051] The second embodiment, as Figures 1-6 As shown, based on the first embodiment:

[0052] A sampling mechanism 4 is used to collect stem cells. The sampling mechanism 4 is installed at the bottom of the housing 2 and close to the frame 1;

[0053] Among them, the sampling mechanism 4 includes a sampler 41 and a conical guide sleeve 42. The sampler 41 is installed at the side of the outer side of the shell 2, and the sampler 41 is installed at a position close to the frame 1. The conical guide sleeve 42 is fixedly installed in the middle of the bottom of the shell 2. A hose 43 is installed in the middle of the bottom of the sampler 41. The hose 43 passes through the center of the conical guide sleeve 42 and extends to the interior of the shell 2. The end of the hose 43 away from the sampler 41 is connected to a material taking round head 44, and a sealing ring 45 is fixedly installed at the feed inlet on the top of the material taking round head 44. A lifting component 46 is installed at the bottom of the inner cavity of the shell 2 and near the material taking round head 44. The lifting component 46 applies a pushing force to the material taking round head 44, so that the material taking round head 44 moves upward, and leaves room in combination with the hose 43, so that the material taking round head 44 moves upward smoothly, and it is not easy to get stuck in the structure, and the upward moving material taking round head 44 coincides with the bottom end of the liquid storage tube 33;

[0054] At the same time, the staff turns on the sampler 41 to work, and uses the suction generated by the sampler 41 and the conveyance of the hose 43 to make the sampling round head 44 suck out the stem cells, so that the sample can be collected.

[0055] The sealing ring 45 is made of rubber, which is convenient for sealing the edge of the feeding port at the top of the feeding round head 44 . There are two groups of lifting components 46 , and the two groups of lifting components 46 are symmetrically installed along the feeding round head 44 .

[0056] The lifting assembly 46 includes a U-shaped bracket 461 and an arched push rod 462. The opening of the U-shaped bracket 461 is downward, and the bottom end of the U-shaped bracket 461 is fixedly installed with the bottom of the inner cavity of the shell 2. An arc hole 463 is provided in the middle of the outer side of the arched push rod 462. The pin shaft at the top of the U-shaped bracket 461 passes through the middle of the arc hole 463. The connecting end of the outer side of the arched push rod 462 is hinged to the bottom of the spherical surface of the material-taking round head 44. A return spring 464 is fixedly installed between the middle of the bottom of the arched push rod 462 and the bottom of the inner cavity of the shell 2. When the output end of the electric telescopic rod 31 is extended, the pressing member 32 is driven to move downward, and the liquid storage tube 33 moves along with it. The lifting rod 462 is pushed down and pressed by the pressing member 32 to move the lifting rod 462 away from the end of the feeding round head 44. Under the support of the U-shaped bracket 461 and the sliding installation of the arc hole 463, the feeding round head 44 is lifted up, and the return spring 464 is stretched. The upward moving feeding round head 44 is embedded in the annular positioning sleeve 342, and the feeding round head 44 applies an upward pushing force to the ball 343, so that the ball 343 moves upward, and the right-angle elastic sheet 341 is compressed, and the discharge hole at the bottom of the liquid storage tube 33 is opened. Under the sealing of the sealing ring 45, it is convenient to take the stem cells at the bottom of the liquid storage tube 33 through the feeding round head 44.

[0057] There are two arched push rods 462, and the two arched push rods 462 are symmetrically installed along the material-taking round head 44. The reset spring 464 is installed directly below the arched push rod 462 to facilitate applying elastic tension to the arched push rod 462, thereby helping the arched push rod 462 to reset.

[0058] The third embodiment, as Figures 1-8 As shown, based on the first and second embodiments:

[0059] The feeding mechanism 5 includes a square box 51 and a discharge port 58. The edge of the bottom of the square box 51 is fixed to the top of the shell 2 by screws. The discharge port 58 is opened at the bottom of the square box 51. The position of the discharge port 58 corresponds to the position of the magnetic blocking ball 36. A reagent feeder 52 is installed on the side of the top of the square box 51. A right-angle tube 53 is installed on the top of the square box 51 and away from the side of the reagent feeder 52. A servo motor 54 is installed in the middle of the top of the square box 51. A rotating roller 55 is rotatably installed in the middle of the inner cavity of the square box 51. The top of the movable roller 55 is fixedly installed with the output end of the servo motor 54 through a coupling, and a mixing blade 56 is fixedly installed in the middle of the outer cylindrical surface of the rotating roller 55. A flipping assembly 57 is installed on the outer cylindrical surface of the rotating roller 55 near the bottom. When the liquid storage tube 33 moves upward, it can drive the magnetic sealing ball 36 to move upward together, and by making the position of the magnetic sealing ball 36 correspond to the position of the discharge port 58, the magnetic sealing ball 36 can be embedded in the inside of the discharge port 58, thereby sealing the discharge port 58, and it is not easy to leak material at will.

[0060] When the mixing of blood and reagent is completed, the electric telescopic rod 31 can be turned on again to work. By extending the output end of the electric telescopic rod 31, the liquid storage tube 33 can be driven to move downward, and the magnetic blocking ball 36 can be driven to move downward, thereby separating the magnetic blocking ball 36 from the discharge port 58, so that the discharge port 58 is opened, and the mixed liquid material flows downward from the discharge port 58 and is promptly collected by the liquid storage tube 33.

[0061] The staff injects blood into the square box 51 through the right-angle tube 53, injects reagent into the square box 51 through the reagent feeder 52, and turns on the servo motor 54 to work. By rotating the output end of the servo motor 54, the rotating roller 55 can be driven to rotate, so that the mixing blade 56 is driven to rotate together, and the rotating mixing blade 56 can be used to mix the blood and reagent in the square box 51.

[0062] The turning assembly 57 includes a supporting rod 571, which is fixedly mounted on the outer circumference of the rotating roller 55 and near the bottom position. A turning plate 572 is rotatably mounted on the outer circumference of the supporting rod 571 and near the rotating roller 55. A torque spring 573 is fixedly mounted between the end of the supporting rod 571 away from the rotating roller 55 and the side of the outer side of the turning plate 572. A magnetic ball 574 is fixedly mounted on the outer side of the turning plate 572 and near the bottom position. The magnetic ball 574 and the magnetic blocking ball 36 have the same magnetic poles. When the rotating roller 55 rotates, it drives the turning assembly 57 to rotate as a whole, and uses the magnetic ball 574 and the magnetic blocking ball 36 to generate a repulsive magnetic force when the two are close to each other. Under the support of the supporting rod 571, the turning plate 572 is subjected to the reverse magnetic thrust to rotate counterclockwise to adjust the angle, and the torque spring 573 is elastically compressed by the torque force.

[0063] As the flipping plate 572 drives the magnetic ball 574 to continue to rotate, the magnetic ball 574 moves away from the magnetic blocking ball 36, the reverse magnetic thrust disappears, and under the elastic force of the torque spring 573, the flipping plate 572 rotates in the opposite direction. In this way, the flipping plate 572 swings back and forth, and the blood and reagents in the square box 51 can be flipped, so that the materials are fully integrated together.

[0064] The turning plates 572 are arc-shaped. There are two turning plates 572 , and the two turning plates 572 are installed symmetrically along the axis of the rotating roller 55 . The supporting round rod 571 passes through the center of the torque spring 573 .

[0065] When in use, first, when the ball 343 is in the initial state, the ball 343 is subjected to the elastic pressing force of the right-angle elastic piece 341, thereby blocking the liquid outlet hole at the bottom end of the liquid storage tube 33;

[0066] The staff turns on the electric telescopic rod 31 and uses the telescopic end of the electric telescopic rod 31 to retract, so that the liquid storage tube 33 is driven to move upward, and the elastic telescopic sleeve 35 is compressed. The position of the magnetic blocking ball 36 corresponds to the position of the discharge port 58, so that the magnetic blocking ball 36 can be embedded in the discharge port 58, thereby blocking the discharge port 58.

[0067] The top of the liquid storage tube 33 is located inside the elastic sleeve 35, so it can be isolated from the outside air and not easily contaminated by the outside air, thereby helping the stem cells to survive.

[0068] At this time, the staff injects blood into the square box 51 through the right-angle tube 53 and injects reagent into the square box 51 through the reagent feeder 52, and turns on the servo motor 54 to work. The output end of the servo motor 54 rotates, which drives the rotating roller 55 to rotate, causing the mixing blade 56 to rotate together. The rotating mixing blade 56 can then mix the blood and reagent in the square box 51.

[0069] At the same time, when the rotating roller 55 rotates, it drives the flipping assembly 57 to rotate as a whole, and when the magnetic ball 574 and the magnetic blocking ball 36 are close to each other, the two generate a repulsive magnetic force, and under the support of the supporting rod 571, the flipping plate 572 is subjected to the reverse magnetic thrust to rotate counterclockwise to adjust the angle, and the torque spring 573 is elastically compressed by the torque force;

[0070] As the flipping plate 572 drives the magnetic ball 574 to continue rotating, the magnetic ball 574 moves away from the magnetic blocking ball 36, the reverse magnetic thrust disappears, and under the elastic force of the torque spring 573, the flipping plate 572 rotates in the opposite direction. In this way, the flipping plate 572 swings back and forth, and the blood and reagents in the square box 51 can be flipped.

[0071] When the blood and reagent are mixed, the electric telescopic rod 31 can be turned on again to work. By extending the output end of the electric telescopic rod 31, the liquid storage tube 33 can be driven to move downward, and the magnetic blocking ball 36 can be driven to move downward, thereby separating the magnetic blocking ball 36 from the discharge port 58, so that the discharge port 58 is opened, and the mixed liquid material flows downward from the discharge port 58 and is promptly collected by the liquid storage tube 33.

[0072] After the mixed liquid material enters the liquid storage tube 33, the material can be left to stand to allow the mixed material to be layered, the stem cells to be separated, and the material to be pre-treated;

[0073] When the output end of the electric telescopic rod 31 is extended, the pressing member 32 is driven to move downward, and the liquid storage tube 33 moves downward together with it, and the pressing member 32 applies a pressing force to the end of the arched push rod 462 away from the feeding round head 44, and under the support of the U-shaped bracket 461 and the sliding installation through the arc hole 463, the feeding round head 44 is pushed up, and the return spring 464 is stretched, and the upward moving feeding round head 44 is embedded in the annular positioning sleeve 342, and the feeding round head 44 applies an upward pushing force to the ball 343, so that the ball 343 moves upward, and the right-angle elastic sheet 341 is compressed, and the discharge hole at the bottom of the liquid storage tube 33 is opened;

[0074] At this time, the lifting component 46 applies a pushing force to the material taking round head 44, so that the material taking round head 44 moves upward, and the hose 43 leaves room, so that the material taking round head 44 moves upward smoothly, and it is not easy to get stuck. The upward moving material taking round head 44 coincides with the bottom end of the liquid storage tube 33;

[0075] At the same time, the staff turns on the sampler 41 to work, and uses the suction generated by the sampler 41 and the conveyance of the hose 43 to make the sampling round head 44 suck out the stem cells, so that the sample can be collected.

[0076] Obviously, the embodiments described are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without making creative efforts should fall within the scope of protection of the present invention. Structures, devices, and operating methods not specifically described and explained in the present invention shall be implemented in accordance with conventional means in the field unless otherwise specified or limited.

Claims

1. A sample collection device for preparing stem cells, characterized in that: include: A frame (1), the frame (1) having a supporting frame body, a housing (2) mounted on the top of the frame (1), and a loading mechanism (5) mounted in the middle of the top of the housing (2); A pretreatment mechanism (3), which is used to stratify blood, and is installed in the middle of the top of the inner cavity of the housing (2); The pretreatment mechanism (3) includes an electric telescopic rod (31), which is installed on the side of the top of the inner cavity of the shell (2), and a pressing piece (32) is fixedly installed on the telescopic end of the electric telescopic rod (31), and a liquid storage tube (33) is fixedly installed on the top of the pressing piece (32), and an opening and closing component (34) is installed on the bottom end of the liquid storage tube (33). An elastic telescopic sleeve (35) is fixedly installed between the outer circular surface of the liquid storage tube (33) and the top of the inner cavity of the shell (2), and a magnetic blocking ball (36) is fixedly installed on the inclined surface of the inner cavity of the liquid storage tube (33) and near the top. A sampling mechanism (4), the sampling mechanism (4) is used to collect stem cells, and the sampling mechanism (4) is installed at the bottom of the housing (2) and close to the frame (1); The sampling mechanism (4) includes a sampler (41) and a conical guide sleeve (42), wherein the sampler (41) is installed on the side of the outer side of the housing (2), the sampler (41) is installed at a position close to the frame (1), the conical guide sleeve (42) is fixedly installed in the middle of the bottom of the housing (2), a hose (43) is installed in the middle of the bottom of the sampler (41), the hose (43) passes through the center of the conical guide sleeve (42) and extends to the inside of the housing (2), the end of the hose (43) away from the sampler (41) is connected to a feeding round head (44), a sealing ring (45) is fixedly installed at the feeding port on the top of the feeding round head (44), and a lifting assembly (46) is installed at the bottom of the inner cavity of the housing (2) and close to the feeding round head (44); The lifting assembly (46) includes a U-shaped bracket (461) and an arched push rod (462), the opening of the U-shaped bracket (461) is downward, and the bottom end of the U-shaped bracket (461) is fixedly installed with the bottom of the inner cavity of the shell (2), an arc-shaped hole (463) is opened in the middle of the outer side of the arched push rod (462), the pin at the top of the U-shaped bracket (461) passes through the middle of the arc-shaped hole (463), and the outer connecting end of the arched push rod (462) is hinged to the bottom of the spherical surface of the material-retrieving round head (44). A return spring (464) is fixedly installed between the middle of the bottom of the arched push rod (462) and the bottom of the inner cavity of the shell (2). When the output end of the electric telescopic rod (31) is extended, the pressing member (32) is driven to move downward, and the liquid storage tube (33) moves downward together, and a pressing force is applied to the end of the arched push rod (462) away from the material-taking round head (44) through the pressing member (32). Under the support of the U-shaped bracket (461) and through the sliding installation of the arc hole (463), the material-taking round head (44) is lifted up.

2. The sample collection device for preparing stem cells according to claim 1, characterized in that: There are two electric telescopic rods (31), and the two electric telescopic rods (31) are symmetrically installed along the liquid storage tube (33), and the liquid storage tube (33) passes through the center of the elastic telescopic sleeve (35).

3. The sample collection device for preparing stem cells according to claim 1, characterized in that: The opening and closing assembly (34) comprises a right-angle elastic piece (341) and an annular positioning sleeve (342), wherein the right-angle elastic piece (341) is fixedly mounted on the inner wall of the liquid storage tube (33) and close to the bottom, and the annular positioning sleeve (342) is fixedly mounted on the bottom end of the liquid storage tube (33), and a ball (343) is fixedly mounted on one end of the right-angle elastic piece (341) away from the inner wall of the liquid storage tube (33).

4. The sample collection device for preparing stem cells according to claim 3, characterized in that: The right-angle elastic pieces (341) are evenly distributed between the spherical surface outside the sphere (343) and the inner wall of the liquid storage tube (33), and the annular positioning sleeve (342) is installed directly below the liquid storage tube (33).

5. The sample collection device for preparing stem cells according to claim 1, characterized in that: The sealing ring (45) is made of rubber. The lifting components (46) are in two groups, and the two groups of lifting components (46) are symmetrically installed along the material taking round head (44).

6. The sample collection device for preparing stem cells according to claim 1, characterized in that: There are two arched push rods (462), and the two arched push rods (462) are symmetrically installed along the material-taking round head (44), and the return spring (464) is installed directly below the arched push rods (462).

7. The sample collection device for preparing stem cells according to claim 1, characterized in that: The feeding mechanism (5) includes a square box (51) and a discharge port (58). The edge of the bottom of the square box (51) is fixed to the top of the housing (2) by screws. The discharge port (58) is opened at the bottom of the square box (51). The position of the discharge port (58) corresponds to the position of the magnetic blocking ball (36). A reagent feeder (52) is installed on the side of the top of the square box (51). The top of the square box (51) is away from the reagent feeder (52). A right-angle tube (53) is installed on one side, a servo motor (54) is installed in the middle of the top of the square box (51), a rotating roller (55) is rotatably installed in the middle of the inner cavity of the square box (51), the top of the rotating roller (55) is fixedly installed with the output end of the servo motor (54) through a coupling, a mixing blade (56) is fixedly installed in the middle of the outer cylindrical surface of the rotating roller (55), and a flipping component (57) is installed on the outer cylindrical surface of the rotating roller (55) near the bottom.

8. The sample collection device for preparing stem cells according to claim 7, characterized in that: The flipping assembly (57) includes a supporting rod (571), which is fixedly mounted on the outer cylindrical surface of the rotating roller (55) near the bottom. A flipping plate (572) is rotatably mounted on the outer cylindrical surface of the supporting rod (571) near the rotating roller (55). A torque spring (573) is fixedly mounted between an end of the supporting rod (571) away from the rotating roller (55) and an outer side of the flipping plate (572). A magnetic ball (574) is fixedly mounted on the outer side of the flipping plate (572) near the bottom. The magnetic ball (574) and the magnetic blocking ball (36) have the same magnetic poles.

9. The sample collection device for preparing stem cells according to claim 8, characterized in that: The flipping plate (572) is arc-shaped, there are two flipping plates (572), and the two flipping plates (572) are symmetrically installed along the axis of the rotating roller (55), and the supporting round rod (571) passes through the center of the torque spring (573).

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