Fat SVF cell culture device

Abstract

The invention discloses a fat SVF cell culture device which comprises a culture box body, a fixed plate is fixed to the position, close to the bottom, in the culture box body, and a movable disc driven by a servo motor is arranged at the top of the fixed plate; empty grooves are annularly formed in the top of the movable disc at equal intervals, silica gel pads are fixed to the bottoms of the empty grooves, and culture dishes fixed through clamping assemblies in the empty grooves are placed on the tops of the silica gel pads. A communicating pipe fixedly penetrates through the top, close to the right side, of the incubator body; through the design, when the cells in the culture dish need to be extracted for observation and research, the cells can be extracted from the culture dish by taking the communicating pipe as a channel without opening the sealing door, so that the internal environment of the incubator body can be isolated from the outside all the time, the cells in the culture dish are prevented from being in contact with the external environment, and the cell extraction efficiency is improved. And the cells are always kept in a relatively stable culture environment.

Description

Technical Field

[0001] The invention relates to the technical field of cell culture devices, in particular to an adipose SVF cell culture device. Background Art

[0002] SVF, scientifically known as "stromal vascular fraction", is an effective ingredient extracted from the patient's own adipose tissue. It contains a variety of cells with repair functions and a cell population formed by a mixture of cytokines. Adipose tissue has been considered an endocrine organ due to its role in energy regulation, inflammatory response and immune response. In addition, it is one of the sources of multifunctional cells with multidirectional differentiation potential, and this multifunctional cell exists in the vascular stromal part of adipose tissue. When studying adipose SVF cells, it is usually necessary to use a culture device to culture adipose SVF cells.

[0003] When using existing culture devices, it is usually necessary to place a culture dish containing cells to be cultured in the culture device for culture. However, during the cell culture process, the staff needs to regularly observe the culture status of the cells. A common method is for the staff to take out the culture dish from the culture device and extract the cells in the culture dish for observation and research. Since the cells need to be in a relatively sealed environment that avoids contact with the outside world, when the culture dish containing the cells is taken out of the culture device, it will be directly exposed to the air, which may cause the cells to be contaminated, thereby causing the cell culture to fail.

[0004] Therefore, in response to this situation, we designed an adipose SVF cell culture device that can isolate the cultured cells from the external environment at all times without affecting sampling and observation. Summary of the invention

[0005] In order to overcome the above-mentioned shortcomings, the present invention aims to provide a technical solution that can solve the above-mentioned problems.

[0006] To achieve the above-mentioned purpose, the present invention provides the following technical solutions: an adipose SVF cell culture device, comprising a culture box body, a fixed plate is fixed inside the culture box body and near the bottom, and a movable plate driven by a servo motor is arranged on the top of the fixed plate; the top of the movable plate is provided with empty grooves at equal distances in an annular shape, a silicone pad is fixed at the bottom of the empty groove, and a culture dish fixed by a clamping assembly inside the empty groove is placed on the top of the silicone pad; a cylinder is installed at the center of the top of the culture box body, a movable plate is fixed to the power output shaft at the bottom of the cylinder, a fluorescent lamp is installed at the center of the bottom of the movable plate, and a sealing block adapted to the culture dish is fixed at equal distances in an annular shape on the bottom of the movable plate; a connecting pipe is fixed through the top of the culture box body and near the right side, the bottom of the connecting pipe movably passes through the movable plate and the sealing block corresponding to the position of the connecting pipe, the bottom of the connecting pipe is located directly above one of the culture dishes, and four sealing flaps made of silicone are fixed in an annular shape on the inner wall of the connecting pipe and near the bottom, and the connecting pipe is sealed after the four sealing flaps are closed together.

[0007] As a further solution of the present invention: each group of the clamping assemblies includes two return springs, two telescopic rods and two clamping plates, and movable cavities are opened on both sides of each of the empty slots, the return spring is fixed in the movable cavity, the other end of the return spring is fixedly connected to the telescopic rod, and the other end of the telescopic rod is movably inserted into the empty slot and fixedly connected to the clamping plate.

[0008] As a further solution of the present invention: the clamping plate is in an arc-shaped structure, and the clamping plate is clamped on the side wall of the culture dish by the resilience of a reset spring.

[0009] As a further solution of the present invention: the servo motor is installed on one side of the inner bottom wall of the incubator body, a driving gear is installed on the power output shaft of the servo motor, a driven gear is meshed on the driving gear, a rotating rod is installed in the middle of the driven gear, the bottom of the rotating rod is rotatably connected to the inner bottom wall of the incubator body, and the top of the rotating rod movably passes through the fixed plate and is fixed at the bottom center position of the movable disk.

[0010] As a further solution of the present invention: a support ring is also fixed to the bottom of the movable disk, and the bottom of the support ring is placed in a guide groove opened on the top of the fixed plate.

[0011] As a further solution of the present invention: a heating plate is installed inside the left side wall, the right side wall and the rear side wall of the incubator body, and a humidifier, a display screen and a controller are installed in sequence from top to bottom on the right side of the incubator body, and the output port of the humidifier is connected to the interior of the incubator body.

[0012] As a further solution of the present invention: positioning rods are fixed on both sides of the bottom of the movable plate, the bottom of the positioning rods movably penetrates the fixed plate, and temperature sensors and humidity sensors are respectively installed on the left and right sides of the bottom of the movable plate.

[0013] As a further solution of the present invention: the temperature sensor and the humidity sensor are connected to the display screen and the controller respectively through wires, and the controller is connected to the humidifier and the heating plate respectively through wires.

[0014] As a further solution of the present invention: the top of the connecting pipe is threadedly connected with a sealing cover.

[0015] As a further solution of the present invention: a sealed door is hinged on the front of the incubator body, and a visual window is installed on the front of the sealed door near the top.

[0016] Compared with the prior art, the present invention has the following beneficial effects:

[0017] 1. In the present application, by designing structures such as a servo motor, a movable disk, a culture dish, a connecting tube and a sealing flap, the incubator body can be closed by a sealing door to isolate the incubator body from the external environment. When it is necessary to extract cells from the culture dish for observation and research, the connecting tube can be used as a channel to extract cells from the culture dish, and there is no need to open the sealing door to extract cells. In this way, the environment inside the incubator body can be isolated from the outside world at all times, preventing the cells in the culture dish from contacting the external environment, ensuring that the cells are always in a relatively stable culture environment, and ensuring successful cell culture.

[0018] 2. In the present application, the culture dish can be stably clamped in the empty slot through the designed clamping assembly. When the movable plate drives the culture dish to change position, the stability of the culture dish on the movable plate can be ensured. Since the clamping distance of the clamping assembly is adjustable, culture dishes of different sizes can also be clamped in the empty slot, thereby improving the applicability of the incubator body.

[0019] 3. In the present application, by designing a heating plate and a humidifier on the incubator body, in conjunction with the designed temperature sensor, humidity sensor and controller, the temperature and humidity conditions inside the incubator body can be monitored in real time. When the temperature and humidity are not suitable, they can be adjusted in time, so that the interior of the incubator body is always in an environment with suitable temperature and humidity, thereby ensuring the normal growth of the cultured cells. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic diagram of the three-dimensional structure of the incubator body of the present invention;

[0021] Figure 2 It is a front cross-sectional structural schematic diagram of the incubator body of the present invention;

[0022] Figure 3 The present invention Figure 2 A is an enlarged structural diagram;

[0023] Figure 4 It is a three-dimensional structural schematic diagram of the movable disk of the present invention;

[0024] Figure 5 It is a three-dimensional structural schematic diagram of the movable plate of the present invention;

[0025] Figure 6 It is a bottom view structural schematic diagram of the movable plate of the present invention;

[0026] Figure 7 1 is a schematic diagram of the top view of the sealing flap of the present invention;

[0027] Figure 8 It is a schematic diagram of the structure when the sealing block of the present invention is covered on the culture dish.

[0028] The reference numerals and names in the figures are as follows:

[0029] 1. Incubator body; 2. Fixed plate; 3. Movable plate; 301. Empty slot; 302. Support ring; 4. Servo motor; 5. Driving gear; 6. Rotating rod; 7. Driven gear; 8. Heating plate; 9. Humidifier; 10. Movable plate; 11. Cylinder; 12. Connecting pipe; 1201. Sealing cover; 1202. Sealing flap; 13. Sealing block; 14. Humidity sensor; 15. Temperature sensor; 16. Fluorescent lamp; 17. Positioning rod; 18. Display screen; 19. Controller; 20. Movable cavity; 21. Reset spring; 22. Telescopic rod; 23. Clamping plate; 24. Silicone pad; 25. Culture dish; 26. Sealing door; 27. Visual window. DETAILED DESCRIPTION

[0030] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0031] See also Figure 1-8A fat SVF cell culture device includes a culture box body 1, a fixed plate 2 is fixed inside the culture box body 1 and near the bottom, a movable plate 3 driven by a servo motor 4 is arranged on the top of the fixed plate 2; the top of the movable plate 3 is annularly provided with empty grooves 301 at equal distances, a silicone pad 24 is fixed at the bottom of the empty groove 301, the silicone pad 24 can provide a relatively soft contact environment for a culture dish 25, so as to place the culture dish 25 and avoid bumping the bottom of the culture dish 25, and a culture dish 2 fixed by a clamping component inside the empty groove 301 is placed on the top of the silicone pad 24 5; A cylinder 11 is installed at the center of the top of the incubator body 1, and a movable plate 10 is fixed to the power output shaft at the bottom of the cylinder 11. A fluorescent lamp 16 is installed at the center of the bottom of the movable plate 10. The bottom of the movable plate 10 is fixed with a sealing block 13 adapted to the culture dish 25 at equal distances in a ring shape. During the cell culture process, if the culture dish 25 needs to be capped, the staff can start the cylinder 11, and the power output shaft of the cylinder 11 pushes the movable plate 10 down, thereby driving the sealing block 13 to descend, so that the sealing block 13 is used to cover the culture dish 25 to achieve sealing of the culture dish 25 (such as Figure 8 As shown), at the same time, a fluorescent lamp 16 is also provided at the bottom of the movable plate 10, which can be turned on when necessary to facilitate cell growth and observation; a connecting pipe 12 is fixedly penetrated at the top of the incubator body 1 and near the right side, the bottom of the connecting pipe 12 movably penetrates the movable plate 10 and a sealing block 13 corresponding to the position of the connecting pipe 12, the bottom of the connecting pipe 12 is located directly above one of the culture dishes 25, and four sealing petals 1202 made of silicone are fixed in a ring shape on the inner wall of the connecting pipe 12 and near the bottom. The four sealing petals 1202 are closed together to seal the connecting pipe 12, and the top of the connecting pipe 12 is threadedly connected to a sealing cover 1201, which can seal the top of the connecting pipe 12.

[0032] See also Figure 1-3 In the present embodiment, each group of clamping assemblies includes two reset springs 21, two telescopic rods 22 and two clamping plates 23. A movable cavity 20 is provided on both sides of each empty slot 301. The reset spring 21 is fixed in the movable cavity 20. The other end of the reset spring 21 is fixedly connected to the telescopic rod 22. The other end of the telescopic rod 22 is movable through the empty slot 301 and is fixedly connected to the clamping plate 23. The clamping plate 23 is an arc-shaped structure. The clamping plate 23 is clamped on the side wall of the culture dish 25 by the resilience of the reset spring 21.

[0033] Specifically, by setting up a clamping assembly, when the culture dish 25 is placed in the empty slot 301, the side wall of the culture dish 25 will squeeze the clamping plate 23, so that the clamping plate 23 can push the telescopic rod 22, and the telescopic rod 22 pushes the reset spring 21 to be compressed, thereby achieving stable clamping of the culture dish 25, and the clamping plate 23 is affected by the rebound effect of the reset spring 21, which can strengthen the clamping effect on the culture dish 25 and further improve the stability of the culture dish 25 in the empty slot 301.

[0034] See also Figure 2 In this embodiment, the servo motor 4 is installed on one side of the inner bottom wall of the incubator body 1, a driving gear 5 is installed on the power output shaft of the servo motor 4, a driven gear 7 is meshed on the driving gear 5, a rotating rod 6 is installed in the middle of the driven gear 7, the bottom of the rotating rod 6 is rotatably connected to the inner bottom wall of the incubator body 1, and the top of the rotating rod 6 movably passes through the fixed plate 2 and is fixed at the bottom center position of the movable disk 3.

[0035] Specifically, by setting up structures such as the servo motor 4, the driving gear 5 and the driven gear 7, when it is necessary to extract cells from the culture dishes 25 at different positions, the servo motor 4 can drive the movable disk 3 to rotate, so that the culture dishes 25 at different positions can come to the bottom of the connecting tube 12, making it convenient for the staff to extract cells from different culture dishes 25 from the connecting tube 12.

[0036] See also Figure 2 and Figure 4 In this embodiment, a support ring 302 is also fixed to the bottom of the movable disk 3 , and the bottom of the support ring 302 is placed in a guide groove opened at the top of the fixed plate 2 .

[0037] Specifically, by setting a support ring 302 at the bottom of the movable disk 3, the support ring 302 slides in the guide groove. When the movable disk 3 rotates, the sliding of the support ring 302 in the guide groove can be used to support and stabilize the movable disk 3, thereby ensuring the stability of the movable disk 3 during rotation.

[0038] See also Figure 1-2 In this embodiment, a heating plate 8 is installed inside the left side wall, the right side wall and the rear side wall of the incubator body 1. A humidifier 9, a display screen 18 and a controller 19 are installed on the right side of the incubator body 1 from top to bottom. The output port of the humidifier 9 is connected to the inside of the incubator body 1; positioning rods 17 are fixed on the left and right sides of the bottom of the movable plate 10, and the bottom of the positioning rod 17 movably penetrates the fixed plate 2. A temperature sensor 15 and a humidity sensor 14 are also installed on the left and right sides of the bottom of the movable plate 10 respectively; the temperature sensor 15 and the humidity sensor 14 are respectively connected to the display screen 18 and the controller 19 through wires, and the controller 19 is respectively connected to the humidifier 9 and the heating plate 8 through wires.

[0039] Specifically, a positioning rod 17 is fixed at the bottom of the movable plate 10. During the descending process of the movable plate 10, on the one hand, the positioning rod 17 can be used to stabilize the descending action of the movable plate 10. On the other hand, when the bottom of the positioning rod 17 contacts the inner bottom wall of the incubator body 1, it is proved that the sealing block 13 covers the top of the culture dish 25, which has the effect of positioning the descending distance of the movable plate 10, thereby preventing the continuous descent of the movable plate 10 from causing damage to the culture dish 25. The temperature sensor 15 can monitor the temperature inside the incubator body 1, and the humidity sensor 14 can monitor the humidity inside the incubator body 1. When the temperature and humidity of the incubator body 1 are not suitable, the temperature sensor 15 and the humidity sensor 14 will transmit signals to the controller 19, and the controller 19 can control the heating plate 8 and the humidifier 9 to adjust the temperature and humidity inside the incubator body 1.

[0040] See also Figure 1 In this embodiment, a sealed door 26 is hinged on the front of the incubator body 1, and a visual window 27 is installed on the front of the sealed door 26 and near the top.

[0041] Specifically, the sealing door 26 can seal the incubator body 1 to ensure that the internal environment of the incubator body 1 is isolated from the outside, and the visual window 27 is installed on the sealing door 26 to facilitate the staff to observe the internal situation of the incubator body 1 from the outside.

[0042] When in use: the staff first needs to place the cells to be cultured in the culture dish 25, and then place the multiple culture dishes 25 in the multiple empty slots 301 on the movable plate 3 in sequence. When the culture dishes 25 are placed, the culture dishes 25 can be stably clamped by the clamping plate 23, and then the sealing door 26 is closed to isolate the internal environment of the incubator body 1 from the outside;

[0043] As the cells are cultured in the culture dish 25, when it is necessary to extract the cells in the culture dish 25 for observation and research, the staff can open the sealing cover 1201 and insert a tool with an extraction effect, such as a syringe, into the connecting tube 12. The extraction end of the syringe squeezes the sealing flap 1202 to open the sealing flap 1202, thereby facilitating the extraction end of the syringe to pass through until the extraction end of the syringe is placed in the culture dish 25. The staff can then use the syringe to extract the cells in the culture dish 25. After the extraction is completed, the syringe can be withdrawn from the connecting tube 12, carrying the extracted cells together. After the syringe is withdrawn, the sealing flap 1202 automatically closes to seal the connecting tube 12, and then the sealing cap 1201 is screwed in to seal the connecting tube 12 again. When the cells are extracted during the culture process, the environment inside the incubator body 1 is never in contact with the outside world, that is, the cells in other culture dishes 25 in the incubator body 1 are never in contact with the outside world. The process of extracting cells uses the connecting tube 12 as a channel, and there is a sealing flap 1202 in the channel to seal the connecting tube 12, thereby ensuring that the cells cultured in the incubator body 1 are always in a relatively stable environment;

[0044] When it is necessary to extract cells from the culture dishes 25 at other positions, the staff only needs to start the servo motor 4, and the power output shaft of the servo motor 4 drives the driving gear 5 to rotate, and the driving gear 5 rotates to drive the driven gear 7 to rotate, and the driven gear 7 rotates to drive the rotating rod 6 to rotate, so that the entire movable disk 3 is driven to rotate through the rotating rod 6. When the movable disk 3 rotates, the culture dishes 25 at different positions can be replaced, so that it is convenient for the staff to extract cells from different culture dishes 25, and the servo motor 4 can set the rotation distance in advance, so as to ensure that the culture dish 25 can come to the bottom of the connecting tube 12 every time the servo motor 4 rotates;

[0045] During the cell culture process, the temperature sensor 15 and the humidity sensor 14 inside the incubator body 1 can monitor the temperature and humidity inside the incubator body 1 in real time. When the temperature is too low, the heating plate 8 heats up to increase the temperature, and when the humidity is too low, the humidifier 9 increases the humidity, thereby ensuring that the interior of the incubator body 1 is always in an environment with a temperature and humidity suitable for cell growth.

[0046] It will be apparent to those skilled in the art that the invention is not limited to the details of the exemplary embodiments described above and that the invention can be implemented in other specific forms without departing from the spirit or essential features of the invention. Therefore, the embodiments should be considered exemplary and non-limiting in all respects, and the scope of the invention is defined by the appended claims rather than the foregoing description, and it is intended that all variations falling within the meaning and scope of the equivalent elements of the claims be included in the invention. Any reference numeral in a claim should not be considered as limiting the claim to which it relates.

Claims

1. A fat SVF cell culture device, characterized in that: The incubator comprises an incubator body (1), wherein a fixed plate (2) is fixed inside the incubator body (1) and near the bottom, and a movable plate (3) driven by a servo motor (4) is arranged on the top of the fixed plate (2); The top of the movable plate (3) is provided with hollow grooves (301) at equal intervals in a ring shape, a silicone pad (24) is fixed at the bottom of the hollow groove (301), and a culture dish (25) is placed on the top of the silicone pad (24) and fixed by a clamping assembly inside the hollow groove (301); A cylinder (11) is installed at the center of the top of the incubator body (1), a movable plate (10) is fixed to the power output shaft at the bottom of the cylinder (11), a fluorescent lamp (16) is installed at the center of the bottom of the movable plate (10), and sealing blocks (13) adapted to the culture dishes (25) are fixed at equal distances in a ring shape at the bottom of the movable plate (10); A connecting pipe (12) is fixedly passed through the top of the incubator body (1) and at a position close to the right side; the bottom of the connecting pipe (12) movably passes through a movable plate (10) and a sealing block (13) corresponding to the position of the connecting pipe (12); the bottom of the connecting pipe (12) is located directly above one of the culture dishes (25); four sealing flaps (1202) made of silicone are fixed in an annular shape on the inner wall of the connecting pipe (12) and at a position close to the bottom; the four sealing flaps (1202) are closed together to seal the connecting pipe (12).

2. The adipose SVF cell culture device according to claim 1, characterized in that: Each group of the clamping assemblies comprises two return springs (21), two telescopic rods (22) and two clamping plates (23); a movable cavity (20) is provided on both sides of each of the empty slots (301); the return spring (21) is fixed in the movable cavity (20); the other end of the return spring (21) is fixedly connected to the telescopic rod (22); the other end of the telescopic rod (22) is movably inserted into the empty slot (301) and is fixedly connected to the clamping plate (23).

3. The adipose SVF cell culture device according to claim 2, characterized in that: The clamping plate (23) is in an arc-shaped structure, and the clamping plate (23) is clamped on the side wall of the culture dish (25) by the resilience of the return spring (21).

4. The adipose SVF cell culture device according to claim 1, characterized in that: The servo motor (4) is mounted on one side of the inner bottom wall of the incubator body (1); a driving gear (5) is mounted on the power output shaft of the servo motor (4); a driven gear (7) is meshed with the driving gear (5); a rotating rod (6) is mounted in the middle of the driven gear (7); the bottom of the rotating rod (6) is rotatably connected to the inner bottom wall of the incubator body (1); the top of the rotating rod (6) movably passes through the fixed plate (2) and is fixed at the bottom center of the movable disk (3).

5. The adipose SVF cell culture device according to claim 1, characterized in that: A support ring (302) is also fixed to the bottom of the movable plate (3), and the bottom of the support ring (302) is placed in a guide groove opened at the top of the fixed plate (2).

6. The adipose SVF cell culture device according to claim 1, characterized in that: A heating plate (8) is installed inside the left side wall, the right side wall and the rear side wall of the incubator body (1). A humidifier (9), a display screen (18) and a controller (19) are installed on the right side of the incubator body (1) from top to bottom. The output port of the humidifier (9) is connected to the interior of the incubator body (1).

7. The adipose SVF cell culture device according to claim 1, characterized in that: Positioning rods (17) are fixed on both left and right sides of the bottom of the movable plate (10), and the bottom of the positioning rods (17) movably penetrates the fixed plate (2). A temperature sensor (15) and a humidity sensor (14) are also respectively installed on the left and right sides of the bottom of the movable plate (10).

8. The adipose SVF cell culture device according to claim 7, characterized in that: The temperature sensor (15) and the humidity sensor (14) are respectively connected to the display screen (18) and the controller (19) through wires, and the controller (19) is respectively connected to the humidifier (9) and the heating plate (8) through wires.

9. The adipose SVF cell culture device according to claim 1, characterized in that: The top of the connecting pipe (12) is threadedly connected with a sealing cover (1201).

10. The adipose SVF cell culture device according to claim 1, characterized in that: A sealed door (26) is hingedly connected to the front of the incubator body (1), and a visual window (27) is installed on the front of the sealed door (26) and near the top.

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