Attractant, lure and trapping device for luring female and male adults of beetle

Abstract

The invention discloses an attractant for attracting female and male adults of Hetrametes. The attractant comprises the following components: 2-Hexenal with the concentration of 1 [mu] g.[ mu] L <-1 >, (E) <->, benzaldehyde, 2-hydroxy <->, o-Xylene and a diluent, wherein the 2-Hexenal, the (E) <->, the benzaldehyde and the 2-hydroxy <-> are single compounds which play a role in attracting male adults, and the o-Xylene is a single compound which plays a role in attracting female adults. The invention further discloses a lure for luring the female and male adult beetles and a trapping device for luring the female and male adult beetles. The attractant disclosed by the invention is strong in attractive force, capable of effectively reducing the use of attractive substances and excellent in trapping effect, and the lure and the trapping device which are prepared from the attractant can be used for effectively monitoring, preventing and treating the leaf peak of the heritosa.

Description

Technical Field

[0001] The present invention relates to the technical field of pest monitoring and trapping, and in particular to an attractant, a trapping core and a trapping device for luring male and female adults of the Hequ Silk Sawfly. Background Art

[0002] White willow is a tree of the Salicaceae family and genus Salix. It is distributed in Xinjiang, China, and is cultivated in Gansu, Qinghai, Tibet and other provinces and regions. It is a commonly used timber and greening tree in my country.

[0003] The Hequ Silk Sawfly, belonging to the order Hymenoptera, family Tetrapodidae, is a herbivorous pest that feeds on the leaves of Salix matsudana, Salix gracile, Salix alba, and Populus beijingensis. The insect was discovered and named in 1990 and is currently only distributed in Liaoning (Zhanggutai), Inner Mongolia (Hulunbuir), Beijing, and Tibet (Lhasa) in China. The Hequ Silk Sawfly causes damage by feeding on leaves as larvae. It has the characteristics of rapid reproduction, high insect population density, large appetite, and severe damage. The Hequ Silk Sawfly was first discovered in Lhasa, Tibet Autonomous Region in September 2016, and has caused huge economic, social harm and environmental impact to the region.

[0004] At present, the prevention and control of the Hequ Silk Sawfly mainly relies on chemical control. Although there are no reports on its resistance to pesticides, the long-term and large-scale use of pesticides will inevitably lead to environmental pollution, the insect's resistance to pesticides, and the harm of drug residues to human health.

[0005] Insect pheromones are trace chemicals secreted by special secretory organs of one sex of the same insect species, which can be received by the receptors of the opposite sex of the same species and cause the opposite sex to produce certain behavioral responses or physiological effects (such as mate seeking, directional courtship, mating, etc.). If these information substances are artificially synthesized and made into attractants, they can be placed in the forest to simulate a certain sex that releases odor, thereby luring the opposite sex to mate, and then capture them, thus achieving the purpose of monitoring and controlling such pests.

[0006] Insect traps are devices used with insect attractants. In order to better attract specific insects, the use of traps for each insect is different, including: trap color, hanging height, hanging position, hanging density, etc.

[0007] At present, there is no relevant research on the plant-based attractants, traps, hanging heights, and lures for female and male adults of the Hequ Silk Sawfly.

[0008] Therefore, an attractant, an attractant core and a trapping device for attracting male and female adults of the Hequ Silk Sawfly are provided. Summary of the invention

[0009] The purpose of the present invention is to overcome the existing defects and provide an attractant, a lure core and a trapping device for luring male and female adults of the Hequ silk-leaf sawfly. The attractant has strong attracting power, can effectively reduce the use of attractant substances, and has excellent trapping effect. The lure core and trapping device prepared by the attractant can effectively monitor and prevent the Hequ silk-leaf sawfly.

[0010] The technical solution to achieve the above purpose is: An attractant for attracting male and female adults of the Hequ Silk Sawfly of the present invention comprises the following components: Concentration: 1 μg·μL -1 2-Hexenal, (E)-, benzaldehyde, 2-hydroxy-, o-Xylene and diluent; Among them, 2-Hexenal, (E)- and benzaldehyde, 2-hydroxy- are single compounds that attract male adults, and o-Xylene is a single compound that attracts female adults.

[0011] Preferably, the diluent is n-hexane.

[0012] Preferably, the invention further comprises: 1% to 2% by weight of an anti-ultraviolet stabilizer and 1% to 2% by weight of an antioxidant.

[0013] The second aspect of the present invention is an attractant core for attracting male and female adults of the Hequ Silk-Sawfly. The attractant cores are respectively a slow-release bottle, a slow-release bag, and an eraser-type attractant core. The slow-release bottle and the slow-release bag are used to load attractants for attracting male and female adults of the Hequ Silk-Sawfly as the attractant core; the eraser-type attractant core comprises: an attractant for attracting male and female adults of the Hequ Silk-Sawfly and an attractant core carrier; the attractant for attracting male and female adults of the Hequ Silk-Sawfly is attached to the attractant core carrier.

[0014] Preferably, the method for making the lure core comprises: The attractant for attracting male and female adults of the Sawfly is dissolved in an organic solvent to prepare an attractant solution; The prepared attractant solution is added dropwise onto the attractant core carrier; After the solvent evaporates, the bait core for attracting the female and male adults of the Hequ Silk Sawfly is prepared.

[0015] The third aspect of the present invention is a trapping device for attracting male and female adults of the Hequ Silk Sawfly, comprising a viscose trap and an attractant core for attracting male and female adults of the Hequ Silk Sawfly, wherein the attractant core is installed on the viscose trap.

[0016] Preferably, the viscose-type trap is a sticky insect board, and the attractant core is installed on the sticky insect board.

[0017] Preferably, the viscose trap is triangular, boat-shaped, or bucket-shaped.

[0018] Preferably, the method for using the trapping device comprises: Before the occurrence of the female and male adults of the Hequ Silk Sawfly, the lure core is installed on the viscose trap to prepare the trapping device; The trapping device is placed in an open area in the forest or at a ventilation hole near the edge of the forest where the female and male adults of the Hequ Silk Sawfly are found; Wherein, the trapping device is suspended at a height of 1-1.5 meters from the ground, and the distance between the trapping devices is 8-10 meters.

[0019] The beneficial effects of the present invention are as follows: the attractant for attracting the female and male adults of the Hequ silk-leaf sawfly provided by the present invention has strong specificity and attracting power, is green and pollution-free, does not harm natural enemies, and is easy to use. The attractant core prepared by the attractant and the matching trapping device can not only achieve the purpose of monitoring and preventing and controlling the female and male adults of the Hequ silk-leaf sawfly, but also can be used for large-scale promotion and application in the forest, reduce the use of chemical pesticides in the forest, and maintain the stability of the ecological environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 In the experiment of the present invention, 0.1 μg·μL -1 The EAG (electroantennary potential) response of 2-Hexenal, (E)- to male adults at different concentrations; Figure 2 In the experiment of the present invention, 1 μg·μL -1 EAG response diagram of 2-Hexenal, (E)- to male adults at different concentrations; Figure 3 In the experiment of the present invention, 10 μg·μL -1 EAG response diagram of 2-Hexenal, (E)- to male adults at different concentrations; Figure 4 In the experiment of the present invention, 100 μg·μL -1 EAG response diagram of 2-Hexenal, (E)- to male adults at different concentrations; Figure 5 In the experiment of the present invention, 0.1 μg·μL -1 The EAG (antennary potential) response graph of male adults to benzaldehyde, 2-hydroxy- at different concentrations; Figure 6 In the experiment of the present invention, 1 μg·μL -1 EAG response diagram of male adults to benzaldehyde, 2-hydroxy- at different concentrations; Figure 7 In the experiment of the present invention, 10 μg·μL -1EAG response diagram of male adults to benzaldehyde, 2-hydroxy- at different concentrations; Figure 8 In the experiment of the present invention, 100 μg·μL -1 EAG response diagram of male adults to benzaldehyde, 2-hydroxy- at different concentrations; Fig. 9 In the experiment of the present invention, 0.1 μg·μL -1 EAG (electroantennary potential) response of female adults to o-Xylene at different concentrations; Fig.10 In the experiment of the present invention, 1 μg·μL -1 EAG response diagram of o-Xylene to female adults at different concentrations; Fig.11 In the experiment of the present invention, 10 μg·μL -1 EAG response diagram of o-Xylene to female adults at different concentrations; Fig.12 In the experiment of the present invention, 100 μg·μL -1 EAG response diagram of o-Xylene to female adults at different concentrations. DETAILED DESCRIPTION

[0021] The technical solution of the present invention will be described clearly and completely below in conjunction with the accompanying drawings. In the description of the present invention, it should be noted that the terms "center", "up", "down", "left", "right", "vertical", "horizontal", "inside", "outside" and the like indicate directions or positional relationships based on the directions or positional relationships shown in the accompanying drawings, which are only for the convenience of describing the present invention and simplifying the description, and do not indicate or imply that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore cannot be understood as a limitation on the present invention. In addition, the terms "first", "second", and "third" are used for descriptive purposes only and cannot be understood as indicating or implying relative importance.

[0022] The present invention will be further described below in conjunction with the accompanying drawings.

[0023] An attractant for attracting male and female adults of Hequ Silk Sawfly, comprising the following components: Concentration: 1 μg·μL -1 2-Hexenal, (E)-, benzaldehyde, 2-hydroxy-, o-Xylene and diluent; Among them, 2-Hexenal, (E)- and benzaldehyde, 2-hydroxy- are single compounds that attract male adults, and o-Xylene is a single compound that attracts female adults.

[0024] In the embodiment, the diluent is n-hexane.

[0025] An attractant for attracting male and female adults of Hequ Silk Sawfly, further comprising: an anti-ultraviolet stabilizer accounting for 1%-2% of the total mass percentage and an antioxidant accounting for 1%-2% of the total mass percentage.

[0026] A lure for attracting male and female adults of Hequ Silk Sawfly, the lure cores are respectively a slow-release bottle, a slow-release bag, and an eraser-type lure core. The slow-release bottle and the slow-release bag are used to load an attractant for attracting male and female adults of Hequ Silk Sawfly as the lure core; the eraser-type lure core comprises: an attractant for attracting male and female adults of Hequ Silk Sawfly and an attractant core carrier; the attractant for attracting male and female adults of Hequ Silk Sawfly is attached to the attractant core carrier.

[0027] In the embodiment, the sustained-release bottle and sustained-release bag are both existing products with a capacity of 16 ml. The models of the sustained-release bottle are: Y, YL, and YL-16ml.

[0028] In the embodiment, the lure carrier is an existing product, such as a rubber-headed empty lure.

[0029] In the embodiment, the method for making the lure core comprises: The attractant for attracting male and female adults of the Hequ Silk Sawfly is dissolved in an organic solvent to prepare an attractant solution; Add the prepared attractant solution dropwise onto the bait core carrier; After the solvent evaporates, the bait core for attracting the female and male adults of the Hequ Silk Sawfly is prepared.

[0030] A trapping device for luring male and female adults of Hequ Silk Sawfly comprises a viscose trap and a lure core for luring male and female adults of Hequ Silk Sawfly, wherein the lure core is installed on the viscose trap.

[0031] In the embodiment, the viscose type trap is a sticky insect board, and the attractant core is installed on the sticky insect board.

[0032] In the embodiment, the viscose-type trap is triangular, boat-shaped, and bucket-shaped, all of which are existing products. For example, the triangular trap model is: YL-D, the boat-shaped trap model is: YL-LWT-1 and YL-WT-2, and the bucket-shaped trap is: BT-L-P2.0.

[0033] In an embodiment, the method for using the trapping device includes: Before the emergence of the male and female adults of the Hequ Silk Sawfly, the lure core is installed on the viscose trap to make a trapping device; Place the traps for the female and male adults of the Hequ Silk Sawfly in open areas of the forest or at ventilation holes near the forest edge; Among them, the hanging height of the trapping device is 1-1.5 meters from the ground, and the distance between the trapping devices is 8-10 meters.

[0034] The attractants, lures and trapping devices for luring male and female adults of the Hequ Silk Sawfly were verified through experiments.

[0035] In the initial stage of the field trapping experiment, three different types of glue traps (triangle, boat, bucket) and three different types of lure cores (slow-release bottle, slow-release bag, eraser) were set up to evaluate their attracting effects on adults of the river bend silk leaf peak.

[0036] For each volatile, regardless of the type of bait it was in and at what concentration (0.1 μg μL -1 , 1μg·μL -1 , 10μg·μL -1 and 100 μg·μL -1 ), a separate experimental group will be set up for research. At the same time, for each type of bait and each set concentration, a CK blank control group will be set up. The purpose of this blank control group is to provide a reference without adding the volatiles to be tested, so as to evaluate the effect of the volatiles by comparison.

[0037] However, the experimental results showed that the bucket trap, eraser-type slow-release lure, and slow-release bag had very weak luring effects, and the total number of adults of the Hequ silk leaf peak lured was very small, and all individual experimental groups were not significant. This finding prompted us to re-evaluate the design and function of the trap and consider other possible improvements. Although this result deviated from our expectations, it provided us with valuable information, helped us optimize the experimental design, and gained a deeper understanding of the biological characteristics of the Hequ silk leaf peak adults.

[0038] In the early stage of the present invention, it was found through preliminary experiments that it was almost impossible to catch the adult of the Hequ Silky Leaf Peak when the trap was hung at a height of more than 1.5 meters. This may be related to the weak flying ability and flying height of the adult of the Hequ Silky Leaf Peak, or it may be because the Hequ Silky Leaf Peak is active at a lower altitude due to food sources, habitat preferences or strategies to escape predators. Therefore, as the hanging height increases, the target insects trapped by the trap may gradually decrease, or even fail to effectively capture the target insects.

[0039] In the case of a boat-shaped viscose trap, the trapping effects of different lures are as follows: At 0.1 μg·μL -1 , 1μg·μL -1 , 10μg·μL-1 and 100 μg·μL -1 Different concentrations of 2-Hexenal, (E)-, benzaldehyde, 2-hydroxy-, and o-Xylene were used to prepare attractants, and then the lure cores were obtained. The EAG reactions of 2-Hexenal, (E)-, benzaldehyde, 2-hydroxy- to male adults and o-Xylene to female adults are shown in the figure. Figure 1-12 shown.

[0040] According to the results, it can be concluded that at 1μg·μL -1 At the same concentration, 2-Hexenal, (E)- had the best effect on attracting male adults, with 265, and only 1 female. benzaldehyde, 2-hydroxy- attracted 71 male adults and 1 female; o-Xylene attracted 62 females, and only 2 male adults.

[0041] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions described in the above embodiments may still be modified, or some or all of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. An attractant for attracting male and female adults of Hequ Silk Sawfly, characterized in that: Includes the following components: Concentration: 1 μg·μL -1 2-Hexenal, (E)-, benzaldehyde, 2-hydroxy-, o-Xylene and diluent; Among them, 2-Hexenal, (E)- and benzaldehyde, 2-hydroxy- are single compounds that attract male adults, and o-Xylene is a single compound that attracts female adults.

2. The attractant for attracting male and female adults of the Hequ Sawfly according to claim 1, characterized in that: The diluent is n-hexane.

3. The attractant for attracting male and female adults of the Hequ Silk Sawfly according to claim 1, characterized in that: Also includes: 1%-2% of the total mass percentage of the anti-ultraviolet stabilizer and 1%-2% of the total mass percentage of the antioxidant.

4. A lure for attracting male and female adults of Hequ Silk Sawfly, characterized in that: The lure cores are respectively a slow-release bottle, a slow-release bag, and an eraser-type lure core, and the slow-release bottle and the slow-release bag are used to load attractants for attracting male and female adults of the Hequ Silk Sawfly, as lure cores; The eraser-type lure core comprises: an attractant for attracting male and female adults of the Hequ Silk Sawfly and a lure core carrier; the attractant for attracting male and female adults of the Hequ Silk Sawfly is attached to the lure core carrier.

5. The attractant core of the attractant for attracting male and female adults of the Hequ Silk Sawfly according to claim 4, characterized in that: The method for making the lure core comprises: The attractant for attracting male and female adults of the Sawfly is dissolved in an organic solvent to prepare an attractant solution; The prepared attractant solution is added dropwise onto the attractant core carrier; After the solvent evaporates, the bait core for attracting the female and male adults of the Hequ Silk Sawfly is prepared.

6. A trapping device for luring male and female adults of Hequ Silk Sawfly, characterized in that: The invention comprises a viscose trap and an attractant core for attracting male and female adults of the Hequ Silk Sawfly, and the attractant core is installed on the viscose trap.

7. A trapping device for luring male and female adults of the Hequ Silk Sawfly according to claim 6, characterized in that: The viscose-type trap is a sticky insect board, and the lure core is installed on the sticky insect board.

8. The trapping device for luring male and female adults of the Hequ Silk Sawfly according to claim 6, characterized in that: The viscose trap is in the shape of a triangle, a boat or a bucket.

9. The trapping device for luring male and female adults of the Hequ Silk Sawfly according to claim 6, characterized in that: The method for using the trapping device comprises: Before the occurrence of the female and male adults of the Hequ Silk Sawfly, the lure core is installed on the viscose trap to prepare the trapping device; The trapping device is placed in an open area in the forest or at a ventilation hole near the edge of the forest where the female and male adults of the Hequ Silk Sawfly are found; Wherein, the trapping device is suspended at a height of 1-1.5 meters from the ground, and the distance between the trapping devices is 8-10 meters.

Images