Tung sulfur multivitamin agent as well as preparation method and application thereof

Abstract

The invention provides a tung sulfur multi-vitamin agent. The tung sulfur multi-vitamin agent is prepared from the following raw materials in parts by weight: 500 to 1500 parts of tung oil, 100 to 200 parts of sulfur and 0 to 50 parts of quick lime. The invention also provides a preparation method and application thereof. Compared with the prior art, the tung sulfur multi-vitamin agent provided by the invention has the following advantages that the tung sulfur multi-vitamin agent is mainly used for preventing and treating gummosis of the economic crop pepper, the cure rate of the gummosis of the pepper can be effectively increased, the morbidity can be effectively reduced, and meanwhile, the tree body can be effectively promoted to grow and mature and the fruit yield can be effectively increased.

Description

Technical Field

[0001] The present application relates to the technical field of economic crop disease prevention and control, and in particular to a tung sulfur multidimensional agent and a preparation method and application thereof. Background Art

[0002] Gummosis is a common branch and trunk disease that occurs widely in Rutaceae and Rosaceae tree species worldwide, which seriously affects the healthy development of the industry of tree species such as Zanthoxylum bungeanum. Zanthoxylum bungeanum is an important spice and medicinal economic forest plant in my country, and has great development and utilization value. While the Zanthoxylum bungeanum industry is developing rapidly, plantations are usually managed in a slack manner, and biological hazards such as microorganisms and insects are also underestimated. Gummosis of branches and trunks of Zanthoxylum bungeanum is a very harmful branch and trunk disease, which mainly harms the base of the trunks of old trees, weakens the Zanthoxylum bungeanum trees, reduces yields, and seriously affects economic and ecological benefits. Research on the prevention and control methods of Zanthoxylum bungeanum gummosis can effectively improve the quality of Zanthoxylum bungeanum trees and increase the yield of Zanthoxylum bungeanum fruits, thereby achieving the goal of improving the economic benefits of the industry, which is of great significance to the development of the Zanthoxylum bungeanum industry.

[0003] Gummosis is divided into two types: infectious and non-infectious. Infectious gummosis is caused by Fusarium, while non-infectious gummosis is mainly caused by insect pests, frost, sun exposure, machine damage, too much or too little water during planting, high soil pH, improper fertilization, too deep planting, excessive pruning, poor grafting, and unsuitable rootstock materials. The more mature means of prevention and control of gummosis in production is chemical control. Systemic fungicides such as carbendazim and methyl thiophanate are commonly used to prevent and control gummosis. These agents can be transmitted through the plant body and inhibit pathogens. However, with the increasing drug resistance of pathogens and the improvement of environmental protection requirements, the research and development and application of new agents are particularly important. Therefore, the selection of agents should be scientific and forward-looking. Summary of the invention

[0004] In view of the above-mentioned technical limitations, the present application proposes a tung sulfur multidimensional agent and a preparation method and application thereof, which overcomes the deficiencies and defects mentioned in the background technology.

[0005] To achieve the above objectives, this application adopts the following technical solutions: The invention point of the present application is to provide a tung oil multidimensional agent, which is prepared from the following raw materials in parts by weight: 500-1500 parts of tung oil, 100-200 parts of sulfur, and 0-50 parts of quicklime.

[0006] Optionally, the above-mentioned tung oil multidimensional agent is prepared from the following raw materials in parts by weight: 1000 parts of tung oil, 170 parts of sulfur, and 30 parts of quicklime.

[0007] Optionally, in the above-mentioned tung oil multivitamin, the tung oil is freshly squeezed raw tung seed oil of the current year; the fineness of the sulfur is selected to be greater than or equal to 600 mesh; and the quicklime is obtained by grinding quicklime blocks into powder and passing through an 80-mesh sieve.

[0008] The second invention of the present application is to provide a method for preparing the above-mentioned tung sulfur multivitamin agent, comprising the following steps: S1. Add 55%-65% of tung oil and all the sulfur in a container, heat over medium heat and cook until the sulfur is completely dissolved, then add 35%-45% of the remaining tung oil and cook over low heat, stirring throughout the cooking process; S2. When white bubbles appear on the surface of the tung oil, continue to boil over low heat, stirring constantly to heat the entire tung oil. S3. When the bubbles on the surface turn brown, continue to cook over medium heat; maintain medium heat until the bubbles turn dark reddish brown and white smoke comes out of the container, then stop heating; continue stirring and cooling until there is no white smoke or bubbles on the surface, add a proportional amount of quicklime powder, and then cook over low heat for 5 minutes, then stop stirring to obtain the tung sulfur multidimensional agent.

[0009] Optionally, in the above preparation method, in step S1, 60% of tung oil is first added, and after the sulfur is dissolved, the remaining 40% of tung oil is added.

[0010] The third invention point of the present application is to provide the application of the above-mentioned tung sulfur multidimensional agent and the tung sulfur multidimensional agent prepared by the above-mentioned preparation method in the prevention and treatment of gummosis.

[0011] Optionally, in the above application, the gummosis control is the control of gummosis in economic forests.

[0012] Optionally, in the above application, the economic forest selected is Zanthoxylum bungeanum.

[0013] Compared with the prior art, this application has the following advantages: The tung sulfur multidimensional agent provided in the present application is mainly used for the prevention and treatment of gummosis of the economic crop Zanthoxylum bungeanum. It can not only effectively improve the cure rate of gummosis of Zanthoxylum bungeanum and reduce the incidence rate, but also effectively promote the growth, maturity and fruit yield of the tree. DETAILED DESCRIPTION

[0014] In order to make the purpose, technical solution and advantages of the present application more clear, the present application is further described in detail below. However, it should be understood that the description here is only used to explain the present application and is not used to limit the scope of the present application.

[0015] Unless otherwise defined, all technical terms and scientific terms used herein have the same meaning as those commonly understood by those skilled in the art of the present application, and the terms used herein in the specification of the present application are only for the purpose of describing specific embodiments and are not intended to limit the present application. The reagents and instruments used herein are all commercially available, and the characterization means involved can refer to the relevant descriptions in the prior art, which will not be repeated herein.

[0016] In order to further understand the present application, the present application is further described in detail below in conjunction with the best embodiment.

[0017] Example 1 The tung oil multidimensional agent is prepared from the following raw materials: 500g of tung oil and 100g of sulfur.

[0018] Among them, tung oil is selected as raw tung seed oil newly squeezed in the current year; the fineness of sulfur is selected as 600 mesh; quicklime is obtained by grinding quicklime blocks into powder and passing through an 80-mesh sieve.

[0019] The preparation method of the tung sulfur multidimensional agent is specifically as follows: S1. First add 55% of tung oil and all the sulfur in a container, heat and cook over medium heat until the sulfur is completely dissolved, then add 45% of the remaining tung oil and cook over low heat, stirring throughout the cooking process; S2. When white bubbles appear on the surface of the tung oil, continue to boil over low heat, stirring constantly to heat the entire tung oil; S3. When the bubbles on the surface turn brown, continue to cook over medium heat; maintain medium heat until the bubbles turn dark red-brown and white smoke comes out of the container, then stop heating to obtain the tung sulfur multivitamin.

[0020] The prepared tung sulfur multidimensional agent is mainly used for preventing and controlling economic forest crops, especially the gummosis of pepper.

[0021] Example 2 The tung oil and sulfur multidimensional agent is prepared from the following raw materials: 1500g tung oil, 200g sulfur, and 50g quicklime.

[0022] Among them, tung oil is selected as raw tung seed oil newly squeezed in the current year; the fineness of sulfur is selected as 800 mesh; quicklime is obtained by grinding quicklime blocks into powder and passing through an 80-mesh sieve.

[0023] The preparation method of the tung sulfur multidimensional agent is specifically as follows: S1. Add 65% of tung oil and all the sulfur in a container, heat and cook over medium heat until the sulfur is completely dissolved, then add 35% of the remaining tung oil and cook over low heat, stirring throughout the cooking process; S2. When white bubbles appear on the surface of the tung oil, continue to boil over low heat, stirring constantly to heat the entire tung oil; S3. When the bubbles on the surface turn brown, continue to cook over medium heat; maintain medium heat until the bubbles turn dark reddish brown and white smoke comes out of the container, then stop heating; continue stirring and cooling until there is no white smoke or bubbles on the surface, add a proportional amount of quicklime powder, and then cook over low heat for 5 minutes, then stop stirring to obtain the tung sulfur multidimensional agent.

[0024] The prepared tung sulfur multidimensional agent is mainly used for preventing and controlling economic forest crops, especially the gummosis of pepper.

[0025] Example 3 The tung oil multidimensional agent is prepared from the following raw materials: 1000g tung oil, 170g sulfur, and 30g quicklime.

[0026] Among them, tung oil is selected as raw tung seed oil newly squeezed in the current year; the fineness of sulfur is selected as 700 mesh; quicklime is obtained by grinding quicklime blocks into powder and passing through an 80-mesh sieve.

[0027] The preparation method of the tung sulfur multidimensional agent is specifically as follows: S1. First add 60% of the ratio of tung oil and all the sulfur in a container, heat and cook over medium heat, heat until the sulfur is completely dissolved, then add 40% of the remaining tung oil, cook over low heat, stirring throughout the cooking process; S2. When white bubbles appear on the surface of the tung oil, continue to boil over low heat, stirring constantly to heat the entire tung oil. S3. When the bubbles on the surface turn brown, continue to cook over medium heat; maintain medium heat until the bubbles turn dark reddish brown and white smoke comes out of the container, then stop heating; continue stirring and cooling until there is no white smoke or bubbles on the surface, add a proportional amount of quicklime powder, and then cook over low heat for 5 minutes, then stop stirring to obtain the tung sulfur multidimensional agent.

[0028] The prepared tung sulfur multidimensional agent is mainly used for preventing and controlling economic forest crops, especially the gummosis of pepper.

[0029] Example 4 Field control: In a pepper garden with a serious gummosis outbreak, 12 plots were randomly selected, each with an area of ​​48 m 2(including 9 to 10 plants) to investigate the gummosis of pepper. Then, the infected pepper trees with basically the same tree vigor were selected, the gummosis spots were scraped off and fresh healthy bark was exposed, and then 4 agents and control water treatment were carried out. The 4 agents were: Tong Su Multivitamin of Example 3, Tong Su Multivitamin of Example 1, 40% carbendazim + 70% thiophanate-methyl + 90% ethyl phosphatide aluminum (concentration 50 times) composite preparation, 22.5% picoxystrobin, 5 plants were treated in each treatment, and repeated 3 times. 10 days after the application of the agent, the disease index and prevention effect were investigated and measured. The control effect of the tested agents on the gummosis of pepper is shown in Table 1.

[0030] Table 1

[0031] As can be seen from Table 1, after 10 days of treatment with the four agents, the disease index decreased, while the control water disease index value increased. The control effects of the four agents were 64.2%, 57.0%, 24.3% and 35.6%, respectively. The control effect order was the Tong Su Multidimensional Agent of Example 3> the Tong Su Multidimensional Agent of Example 1 (without quicklime)> 22.5% of picoxystrobin> 40% carbendazim + 70% thiophanate-methyl + 90% ethyl phosphate aluminum (50 times the concentration) composite preparation, and the Tong Su Multidimensional Agent of Example 3 (with quicklime) had the best control effect, which was 1.1 times the control effect of the Tong Su Multidimensional Agent of Example 1 (without quicklime), 1.8 times of 22.5% of picoxystrobin, and 2.6 times of the composite preparation of 40% carbendazim + 70% thiophanate-methyl + 90% ethyl phosphate aluminum (50 times the concentration). It can be concluded that the Tong Su Multidimensional Agent provided in this application significantly reduced the incidence index of pepper gummosis.

[0032] Example 5 Evaluation of the control effect on potted plants: Preparation of spore suspension of Fusarium thunbergii, the pathogen of pepper gum disease (1.0×10 6 CFU·mL -1). One-year-old healthy Zanthoxylum bungeanum seedlings with basically the same growth conditions were selected, with one plant per pot. Use an inoculation needle and a syringe to inject 10 ml of three-line Fusarium spore suspension into the trunk of the Zanthoxylum bungeanum seedlings. After the disease occurs, the four fungicides of Example 3, Example 1, 40% carbendazim + 70% thiophanate-methyl + 90% ethyl phosphatide aluminum (50 times the concentration) composite preparation, and 22.5% picoxystrobin are applied to the lesions. Sterile water is used as a control. Each agent is used to treat 15 plants, and each plant is repeated. Normal water and fertilizer management was carried out during the experiment. The tissues around 0.5-1 cm of the fungicide application were taken at 0d, 1d, 3d, 5d, and 7d after treatment, and the hydrogen peroxide content, malondialdehyde content, and phenylalanine ammonia lyase activity were measured. Three plants were taken at each time point. The results of the effects of different agent treatments on the physiological and biochemical indicators of gummosis of Zanthoxylum bungeanum seedlings are shown in Table 2.

[0033] Table 2

[0034] According to the data in Table 2, after the main trunk of the pepper seedlings was inoculated, the hydrogen peroxide content, malondialdehyde content and phenylalanine ammonia lyase activity in the surrounding tissues and control tissues of the bactericidal agent were measured. After the agent treatment, the hydrogen peroxide content and malondialdehyde content of the pepper tissue were both on a downward trend, while the clear water control continued to increase, and the hydrogen peroxide content and malondialdehyde content of the Tong sulfur multidimensional agent treatment in Example 3 were significantly higher than those of other treatments. The phenylalanine ammonia lyase activity of the pepper tissues treated in each treatment showed a gradual upward trend, and the four agent treatments were significantly higher than the clear water control, and the phenylalanine ammonia lyase activity at each time point of the Tong sulfur multidimensional agent treatment in Example 3 was higher than that of the other three agent treatments, and there was a significant difference. It can be concluded that the Tong sulfur multidimensional agent provided in the present application significantly enhances the disease resistance of pepper to gummosis at the physiological and biochemical level.

[0035] Example 6 Evaluation of the impact on pepper yield: For the five treatments in the field pepper garden of Example 4, the yield of the test pepper variety "Wudu Dahongpao (with thorns)" was measured in September 2022 and 2023 (pepper harvest period), and the results of the measurement of the effects of different agent treatments on pepper yield are shown in Table 3.

[0036] Table 3

[0037] According to Table 3, the yield of Zanthoxylum bungeanum in the five treatments is quite different. After treatment with the composite preparation of 40% carbendazim + 70% thiophanate-methyl + 90% ethyl phosphazene, the yield in 2023 is lower than that in 2022, while the yield in 2023 after treatment with the Tong Su Multivitamin Agent group in Example 3, the Tong Su Multivitamin Agent group in Example 1, and the 22.5% picoxystrobin group is higher than that in 2022. The yield measurement results for two consecutive years showed that the yield of Zanthoxylum bungeanum after treatment with the four agents was significantly higher than that of the control (clear water), among which the Tong Su Multivitamin Agent group in Example 3 was the highest, and was significantly different from other treatments. It can be seen that the Tong Su Multivitamin Agent provided in this application can significantly increase the yield of Zanthoxylum bungeanum after curing gummosis.

[0038] Example 7 The fresh Zanthoxylum bungeanum fruits picked from the five treatments in the field Zanthoxylum bungeanum garden of Example 4 were tested for pesticide residues by high performance liquid chromatography. The test results of pesticide residues in Zanthoxylum bungeanum fruits treated with different agents are shown in Table 4.

[0039] Table 4

[0040] As can be seen from Table 4, no pesticide residues were found in the samples of fresh pepper fruits treated with the Tong Su Multivitamin Agent Group of Example 3 and the Tong Su Multivitamin Agent Group of Example 1 for two consecutive years, while a small amount of pesticide residues were found in the pepper samples treated with the composite preparation group of 40% carbendazim + 70% thiophanate-methyl + 90% ethyl phosphatide aluminum and the 22.5% picoxystrobin group. It can be concluded that the Tong Su Multivitamin Agent provided in this application has no pesticide residues in the pepper fruits after curing gummosis, which improves the safety of fruit consumption and shows green and environmental protection.

[0041] The above description is only a preferred embodiment of the present application and is not intended to limit the present application. Any modifications, equivalent substitutions or improvements made within the spirit and principles of the present application should be included in the protection scope of the present application.

Claims

1. A tung sulfur multivitamin agent, characterized in that: The tung oil-sulfur multidimensional agent is prepared from the following raw materials in parts by weight: 500-1500 parts of tung oil, 100-200 parts of sulfur, and 0-50 parts of quicklime.

2. A tung sulfur multidimensional agent according to claim 1, characterized in that, The tung oil-sulfur multidimensional agent is prepared from the following raw materials in parts by weight: 1000 parts of tung oil, 170 parts of sulfur, and 30 parts of quicklime.

3. A tung sulfur multivitamin according to claim 1 or 2, characterized in that, The tung oil is freshly squeezed tung seed oil of the current year; the fineness of the sulfur is selected to be greater than or equal to 600 meshes; and the quicklime is obtained by grinding quicklime blocks into powder and passing through an 80-mesh sieve.

4. The preparation method of the tung sulfur multidimensional agent according to any one of claims 1 to 3, characterized in that: The following steps are involved: S1. Add 55%-65% of tung oil and all the sulfur in a container, heat over medium heat and cook until the sulfur is completely dissolved, then add 35%-45% of the remaining tung oil and cook over low heat, stirring throughout the cooking process; S2. When white bubbles appear on the surface of the tung oil, continue to boil over low heat, stirring constantly to heat the entire tung oil; S3. When the bubbles on the surface turn brown, continue to cook over medium heat; maintain medium heat until the bubbles turn dark reddish brown and white smoke comes out of the container, then stop heating; continue stirring and cooling until there is no white smoke or bubbles on the surface, add a proportional amount of quicklime powder, and then cook over low heat for 5 minutes, then stop stirring to obtain the tung sulfur multidimensional agent.

5. The preparation method according to claim 4, characterized in that: In step S1, 60% of tung oil is first added, and after the sulfur is dissolved, the remaining 40% of tung oil is added.

6. Use of the tung sulfur multidimensional agent described in any one of claims 1 to 3 or the tung sulfur multidimensional agent prepared by the preparation method described in any one of claims 4 to 5 in the prevention and treatment of gummosis.

7. The use according to claim 6, characterized in that: The gummosis control is the gummosis control of economic forests.

8. The use according to claim 7, characterized in that: The economic forest selected is Zanthoxylum bungeanum.

Images