Application of Huoxiang Anwei Powder in sobering up and preparing products for preventing, treating and / or treating liver and stomach damage caused by alcohol

Abstract

The present invention belongs to the field of medical technology, and specifically relates to the use of Huoxiang Anwei Powder in sobering up, preparing products for preventing, treating and / or treating liver and stomach damage caused by alcohol. The Huoxiang Anwei Powder of the present invention has a significant protective effect on alcohol-induced acute drunkenness and related liver and stomach damage, and this protective effect is related to the fact that Huoxiang Anwei Powder enhances ethanol metabolism, improves antioxidant capacity, reduces lipid peroxidation and inhibits inflammatory response. The formula of Huoxiang Anwei Powder of the present invention has high dose efficiency and synergistic effect, works on multiple levels, and provides more comprehensive protection.

Description

Technical Field

[0001] The invention belongs to the field of medical technology, and specifically relates to the application of Huoxiang Anwei Powder in sobering up and preparing products for preventing, treating and / or curing liver and stomach damage caused by alcohol. Background Art

[0002] Drinking alcohol in the short term may lead to decreased cognitive and motor coordination abilities and increase the risk of trauma. Long-term drinking can lead to a variety of chronic problems, such as alcohol dependence, liver disease (fatty liver, cirrhosis), cardiovascular damage, increased cancer risk, etc. Alcohol-dependent people will have increased tolerance and need more alcohol to achieve the same effect. Symptoms such as anxiety, tremors, and epilepsy may occur during withdrawal. Alcohol abuse may also lead to mental disorders, such as hallucinations and delusions.

[0003] Many alcohol-detoxifying drugs on the market cannot really help the body metabolize alcohol. They may contain diuretic, analgesic and stimulant ingredients, which can temporarily relieve the symptoms of drunkenness, but they do not really help the metabolism of alcohol. Some alcohol-detoxifying drugs may just be placebos, giving people psychological hints. Therefore, it is important to seek drugs that can detoxify alcohol and protect the liver. Summary of the invention

[0004] The purpose of the present invention is to provide the use of Huoxiang Anwei Powder in sobering up, preparing products for preventing, treating and / or treating liver and stomach damage caused by alcohol. Huoxiang Anwei Powder has a significant protective effect on alcohol-induced acute drunkenness and related liver and stomach damage.

[0005] The invention provides application of Huoxiang Anwei Powder in preparing a medicine for preventing and / or treating liver and stomach damage caused by alcohol.

[0006] As a preferred embodiment, based on the body weight of mice, the effective dose of the Huoxiang Anwei Powder is 35-160 mg / kg.

[0007] As a preferred embodiment, the efficacy of the drug includes at least one of the following:

[0008] 1) Reduce the concentration of ethanol in the blood;

[0009] 2) Prevent and treat liver damage caused by ethanol;

[0010] 3) Prevent and treat gastric function damage caused by ethanol;

[0011] 4) Enhance the activity of acetaldehyde dehydrogenase in the liver.

[0012] As a preferred embodiment, the biochemical indicators of liver damage include: triglyceride content and / or glutathione.

[0013] As a preferred embodiment, the biochemical index of gastric function damage includes: catalase activity.

[0014] As a preferred embodiment, the dosage form of the Huoxiang Anwei Powder includes at least one of the following: powder, capsule, tablet, decoction, pill, paste and granule.

[0015] As a preferred embodiment, the Huoxiang Anwei Powder comprises the following raw materials in parts by weight: 40-60 parts of patchouli, 40-60 parts of cloves, 40-60 parts of ginseng and 80-120 parts of tangerine peel.

[0016] The invention provides the use of Huoxiang Anwei Powder in preparing a health food having an auxiliary protective effect on alcoholic liver damage.

[0017] The invention provides application of Huoxiang Anwei Powder in preparing a hangover-relief medicine.

[0018] The invention provides application of Huoxiang Anwei Powder in preparing a hangover-relieving medicine.

[0019] Beneficial effects: The purpose of the present invention is to provide the use of Huoxiang Anwei Powder in sobering up, preparing drugs for preventing, treating and / or treating alcohol-induced liver and stomach damage. The Huoxiang Anwei Powder of the present invention has a significant protective effect on alcohol-induced acute drunkenness and related liver and stomach damage, and this protective effect is related to the fact that Huoxiang Anwei Powder enhances ethanol metabolism, improves antioxidant capacity, reduces lipid peroxidation and inhibits inflammatory response. The formula of Huoxiang Anwei Powder of the present invention has high dose efficiency and synergy, plays a role at multiple levels, and provides more comprehensive protection. It is particularly noteworthy that due to the optimization of the extraction process, even if the same amount of crude drugs is required to exert the efficacy, the dosage of Huoxiang Anwei Powder is much smaller than that of most Chinese medicine extracts or powders. Such advantages can improve patients' medication compliance, reduce dose-dependent side effects, facilitate preparation, improve bioavailability, and facilitate dosage adjustment. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 This is the oil red staining picture of the control group;

[0021] Figure 2 This is the oil red staining picture of the model group;

[0022] Figure 3 This is the oil red staining picture of the Neptune Jinzun group;

[0023] Figure 4 This is the oil red staining picture of the high-dose group of Huoxiang Anwei Powder;

[0024] Figure 5 This is the oil red staining picture of the medium dose group of Huoxiang Anwei Powder;

[0025] Figure 6 This is the oil red staining picture of the low-dose group of Huoxiang Anwei Powder;

[0026] Figure 7HE staining of the liver in the control group;

[0027] Figure 8 HE staining of the stomach in the control group;

[0028] Fig. 9 HE staining of liver in the model group;

[0029] Fig.10 HE staining of the stomach in the model group;

[0030] Fig.11 This is the HE staining picture of the liver of the Haiwangjinzun group;

[0031] Fig.12 This is the HE staining picture of the stomach in the Haiwangjinzun group;

[0032] Fig.13 This is the HE staining picture of the liver in the high-dose group of Huoxiang Anwei Powder;

[0033] Fig.14 This is the HE staining picture of the stomach in the high-dose group of Huoxiang Anwei Powder;

[0034] Fig.15 This is the HE staining picture of the liver in the Huoxiang Anwei Powder medium dose group;

[0035] Fig.16 This is the HE staining picture of the stomach in the medium-dose group of Huoxiang Anwei Powder;

[0036] Fig.17 This is the liver HE staining picture of the low-dose group of Huoxiang Anwei Powder;

[0037] Fig.18 This is the HE staining image of the stomach in the low-dose group of Huoxiang Anwei Powder. DETAILED DESCRIPTION

[0038] The invention provides application of Huoxiang Anwei Powder in preparing a medicine for preventing and / or treating liver and stomach damage caused by alcohol.

[0039] Unless otherwise specified, the present invention has no special requirements for the preparation raw materials, and commercially available products known to those skilled in the art can be used.

[0040] The Huoxiang Anwei Powder of the present invention may include the following raw materials in parts by weight: 40-60 parts of patchouli, 40-60 parts of cloves, 40-60 parts of ginseng and 80-120 parts of tangerine peel, such as 50 parts of patchouli, 50 parts of cloves, 50 parts of ginseng and 100 parts of tangerine peel. In addition, the mass ratio of patchouli, cloves, ginseng and tangerine peel can be 1:1:1:2.

[0041] The preparation method of Huoxiang Anwei Powder of the present invention may include the following steps: Weigh patchouli, cloves, ginseng and tangerine peel according to a mass ratio of 1:1:1:2, reflux separation to obtain volatile oil and water extract; Concentrate the water extract and freeze-dry to obtain freeze-dried powder; Mix the volatile oil and freeze-dried powder to obtain Huoxiang Anwei Powder. The reflux temperature of the present invention can be 95~105℃, such as 100℃, the number of refluxes can be 2~4 times, such as 3 times, and each reflux can be 0.5~1.5h, such as 1h. Before the reflux of the present invention, it can also include: crushing patchouli, cloves, ginseng and tangerine peel into coarse powder. As a specific embodiment, the particle size of the coarse powder can all pass through the No. 2 sieve, and is mixed with powder that can pass through the No. 4 sieve of no more than 40%. The amount of water added during the reflux of the present invention can be 9~11 times the mass of the coarse powder, such as 10 times. The concentration described in the present invention may be reduced pressure concentration, exemplified by vacuum concentration in a water bath at 50-60°C, such as 56°C. The concentration described in the present invention may be to a thick extract, and the water content of the thick extract may be 0.5-1.5 g / mL, such as 1 g / mL. The freeze-drying temperature described in the present invention may be -45--35°C, such as -40°C; the freeze-drying time may be 2-3 days. The present invention may mix the obtained volatile oil and freeze-dried powder, and the volume mass ratio of the volatile oil and freeze-dried powder may be 0.5-0.9 mL: 6-10 g, for example 0.67 mL: 7.5 g, to obtain Huoxiang Anwei Powder.

[0042] In the present invention, based on the weight of mice, the effective dose of the Huoxiang Anwei Powder can be 35~160mg / kg, such as 70~140mg / kg. The clinical dosage of the Huoxiang Anwei Powder in the present invention can be 12~18g / day (original dosage), calculated at 18g / 70kg body weight, 18g / day is about 256mg / kg of Huoxiang Anwei Powder. When the extract yield is 6%, the conversion factor between mice and humans is 9.1 calculated by body surface area, so the mouse dose is 256mg / kg×9.1×0.06≈140mg / kg. The Huoxiang Anwei Powder of the present invention has a significant protective effect on alcohol-induced acute drunkenness and related liver and stomach damage, and this protective effect may be related to the fact that Huoxiang Anwei Powder enhances ethanol metabolism, improves antioxidant capacity, reduces lipid peroxidation and inhibits inflammatory response.

[0043] The efficacy of the drug of the present invention may include at least one of the following: 1) reducing the concentration of ethanol in the blood; 2) preventing and treating liver damage caused by ethanol; 3) preventing and treating gastric damage caused by ethanol; 4) enhancing the activity of liver acetaldehyde dehydrogenase. The biochemical index of liver damage of the present invention may include: triglyceride content and / or glutathione. The biochemical index of gastric damage of the present invention may include: catalase activity.

[0044] The dosage form of the Huoxiang Anwei Powder of the present invention may include at least one of the following: powder, capsule, tablet, decoction, pill, paste and granule.

[0045] The invention provides the use of Huoxiang Anwei Powder in preparing a health food having an auxiliary protective effect on alcoholic liver damage.

[0046] The present invention provides the use of Huoxiang Anwei Powder in the preparation of a hangover-relief drug. The present invention provides the use of Huoxiang Anwei Powder in the preparation of a hangover-relief drug. The Huoxiang Anwei Powder of the present invention can prolong the latent time of drunkenness and significantly shorten the drunkenness time.

[0047] To further illustrate the present invention, the application of the Huoxiang Anwei Powder provided by the present invention in sobering up, preparing products for preventing, treating and / or treating alcohol-induced liver and stomach damage is described in detail below in conjunction with the examples, but they should not be construed as limiting the scope of protection of the present invention.

[0048] Example 1

[0049] Take 500g of patchouli, 500g of cloves, 500g of ginseng and 1000g of tangerine peel, mix them, grind them into coarse powder (that is, they can all pass through the No. 2 sieve, but are mixed with no more than 40% of powder that can pass through the No. 4 sieve), add 10 times the weight of distilled water, boil (100℃) and heat to reflux extraction. Repeat the reflux extraction 3 times, each time for 1 hour. Collect the volatile oil and separate the water extract at the same time. Vacuum the water extract in a 56℃ water bath to reduce pressure and concentrate it to a thick extract (water content of about 1g / mL), freeze-dry it at -40℃ for 2 days to prepare a lyophilized powder. Mix all the volatile oils and lyophilized powder to make Huoxiang Anwei Powder.

[0050] Example 2

[0051] 48 healthy SPF-grade ICR male mice were purchased from Zhuhai Baishitong Biotechnology Co., Ltd., license number: SCXK (Guangdong) 2020-0051, and were kept in the Institute of Biological and Medical Engineering of Guangdong Academy of Sciences, license number: SYXK (Guangdong) 2023-0183. The temperature of the animal laboratory was 18-22°C, and the relative humidity was 55%-85%. The light and dark cycles were alternating for 12 hours each, and the mice were free to take water and feed.

[0052] After seven days of adaptive feeding, 48 male ICR mice were randomly divided into a control group, a model group, a Haiwang Jinzun group, a high-dose Huoxiang Anwei San group, a medium-dose Huoxiang Anwei San group, and a low-dose Huoxiang Anwei San group.

[0053] The Haiwang Jinzun (Haiwang brand Haiwang Jinzun liver protection tablets) group (1.8g / mouse kg), low dose (35mg / mouse kg), medium dose (70mg / mouse kg), and high dose (140mg / mouse kg) groups were continuously gavaged for 7 days, once a day, and the control group and model group were gavaged with the same amount of pure water for 7 days, once a day. 5h after the last gavage, the body weight was weighed. The model group and the low, medium, and high dose groups of Huoxiang Anwei San were all gavaged with 50% (v / v) anhydrous ethanol (Guangzhou Chemical Reagent Factory, item number: HB15) as a modeling agent, and mice were gavaged at a dose of 18mL / kg, and 0.2mL of normal saline was injected subcutaneously on the back. The control group was treated in the same way, with 50% anhydrous ethanol replaced by pure water.

[0054] The mice were considered drunk if they stayed in the same place for more than 10 minutes. The intoxication latency period was defined as the time from the intoxication state to the resumption of activity after oral administration of 50% anhydrous ethanol. The intoxication latency period and intoxication time of each group of mice were recorded. The timing was started from the time of oral administration of 50% anhydrous ethanol until the mice sobered up. The results are shown in Table 1.

[0055] Table 1 Effects of different treatments on intoxication latency and intoxication time

[0056]

[0057] Note: x̄±SEM, N=8. “#” indicates that compared with the model group, P <0.05; “##” indicates that compared with the model group, P <0.01; “###” indicates that compared with the model group, P <0.001.

[0058] From the results in Table 1, compared with the model group, the high and medium dose groups of Huoxiang Anwei San significantly prolonged the incubation period of drunkenness and shortened the duration of drunkenness; compared with the model group, the drunkenness duration of Haiwang Jinzun group was significantly shortened. The protective effect of Huoxiang Anwei San on the acute drunkenness mouse model induced by 50% anhydrous ethanol was evaluated. The results showed that compared with the model group, the high and medium dose groups of Huoxiang Anwei San significantly prolonged the incubation period of drunkenness and shortened the duration of drunkenness in mice, while reducing the ethanol concentration in the blood.

[0059] Example 3

[0060] After seven days of adaptive feeding, mice were gavaged with drugs or pure water according to the groups. 5 hours after the last drug administration, all groups except the control group were given 50% (v / v) anhydrous ethanol to make models. After 12 hours, samples were collected, that is, the eyeballs of mice were removed to collect blood, the liver tissue and stomach tissue were taken out respectively, and the residual blood on the surface was washed with physiological saline. The stomach tissue was cut open along the greater curvature of the stomach and the residue was washed with physiological saline, and the water of the four organs was absorbed by filter paper.

[0061] Detection indicators:

[0062] 1. Blood ethanol concentration detection

[0063] Blood was collected from the eyeball, and 120 μL of whole blood was reserved for freezing and used to detect ethanol content. The detection method was gas chromatography, and the specific conditions were Agilent DB-624 capillary column (30m×0.53mm×3μm), the starting temperature was 40℃, maintained for 5min, heated to 52℃ at a rate of 3℃ per minute, and heated to 240℃ at a rate of 35℃ per minute; the injection port temperature was 240℃, and the detector temperature was 240℃. The injection volume was 1μL, and the split ratio was 5:1. The retention time of ethanol was 4.1min. The results of blood ethanol concentration detection are shown in Table 2.

[0064] Table 2 Effects of different treatments on blood ethanol concentration

[0065]

[0066] Note: x̄±SEM, N=8. “#” indicates that compared with the model group, P <0.05.

[0067] From the results in Table 2, compared with the model group, the blood ethanol concentration in the high and medium dose groups of Huoxiang Anwei San was significantly reduced.

[0068] 2. Determination of organ biochemical indicators

[0069] Dissect an appropriate amount of liver tissue from each mouse, rinse with pre-cooled saline, and prepare 10% (10 mg tissue plus 1 mL saline) liver tissue with a homogenizer, put it into a centrifuge, set the temperature to 4°C, the time to 15 min, and the speed to 4000 r / min, separate the supernatant and place it in a centrifuge tube, and perform the test according to the instructions of the kit. Prepare gastric tissue, heart tissue, and brain tissue test samples in the same way, and keep the remaining tissue for testing.

[0070] Liver tissue detection indicators: The triglyceride (TG) content was detected using the Amplex Red triglyceride detection kit (Biyuntian, catalog number: S0219S), and the glutathione (GSH) was detected using the reduced glutathione (GSH) assay kit (Nanjing Jiancheng, catalog number: A006-2-1). Gastric tissue detection indicators: The catalase (CAT) assay kit (Nanjing Jiancheng, catalog number: A007-1-1) was used to detect the activity of catalase (CAT). The test results of liver and stomach tissue biochemical indicators are shown in Table 3.

[0071] Table 3 Effects of different treatments on biochemical parameters of liver and stomach tissues

[0072]

[0073] Note: x̄±SEM, N=8. “***” means compared with the control group, P <0.001; “##” indicates that compared with the model group, P <0.01; "*" indicates that compared with the control group, P <0.05.

[0074] From the results in Table 3, compared with the control group, the CAT activity in the stomach of the model group was significantly weakened, the TG content in the liver was significantly increased, and the GSH content in the liver was significantly decreased.

[0075] Compared with the model group, the CAT activity in the stomach of the medium and low dose groups of Huoxiang Anwei San was significantly enhanced, the TG content in the liver of the high, medium and low dose groups of Huoxiang Anwei San was significantly decreased, and the GSH content in the liver of the high dose group of Huoxiang Anwei San was significantly increased; compared with the model group, the TG content in the liver of the Haiwang Jinzun group was significantly decreased.

[0076] 3. Organ pathology examination

[0077] After frozen sections of the left lobe of mouse liver tissue were obtained, the liver fat was stained with Oil Red O staining solution (Fuzhou Feijing Biotechnology Co., Ltd., Catalog No.: PH1226). The fat area and liver area in the field of view were counted using Image J, and the liver fat area ratio was calculated. The results are shown in Tables 4 and Figure 1 to Figure 6 .

[0078] Liver fat area ratio (%) = fat area in the microscopic field of view / liver area × 100%.

[0079] The left lobe of the mouse liver and stomach tissue were taken and fixed in 4% (w / v) paraformaldehyde solution, and paraffin sections were made. Hematoxylin staining solution (Zhuhai Besuo Biotechnology Co., Ltd., catalog number: BA4021) and eosin staining solution (Zhuhai Besuo Biotechnology Co., Ltd., catalog number: BA4022) were mixed in a volume ratio of 1:1 to obtain hematoxylin-eosin (HE) staining solution, which was stained with an automatic staining machine (instrument model: TB-180II, manufacturer: Wuhan Taiwei Technology Co., Ltd.). The cells were placed under an optical microscope (instrument model: Mingmei ML51, manufacturer: Guangzhou Mingmei Optoelectronics Technology Co., Ltd.) to scan and record the morphological differences, inflammation and other pathological changes of each group of cells. The results are shown in the figure. Figure 7 to Figure 18 .

[0080] Table 4 Effects of different treatments on liver fat area ratio

[0081]

[0082] Note: x̄±SEM, N=8. “**” indicates that compared with the control group, P <0.01; “##” indicates that compared with the model group, P<0.01.

[0083] from Figure 1 to Figure 18 According to the results in Table 4, compared with the control group, the proportion of liver fat area in the model group was significantly increased. Compared with the model group, the proportion of liver fat area in the high-dose group of Huoxiang Anwei San was significantly reduced. Compared with the control group, the degree of gastric pathological damage in the model group was significantly increased. Compared with the model group, the degree of gastric pathological damage in the high- and medium-dose groups of Huoxiang Anwei San was significantly reduced; compared with the model group, the degree of gastric pathological damage in the Haiwang Jinzun group was significantly reduced.

[0084] Furthermore, the gastric damage after different treatments was evaluated based on the slice results and the scoring criteria in Table 5. The categories of gastric pathological damage were: gastric mucosal epithelial hemorrhage / necrosis / sloughing, interstitial fibrous tissue hyperplasia, and neutrophil infiltration in the local mucosal layer / submucosal layer / muscular layer. The results are shown in Table 6.

[0085] Table 5 Gastric injury pathology scoring table

[0086]

[0087] Table 6 Pathological scoring results of gastric injury under different treatments

[0088]

[0089] Note: x̄±SEM, N=8. “***” means compared with the control group, P <0.001; “##” indicates that compared with the model group, P <0.01.

[0090] From the results in Table 6, compared with the control group, the degree of gastric pathological damage in the model group was significantly increased. Compared with the model group, the degree of gastric pathological damage in the high and medium dose groups of Huoxiang Anwei San was significantly reduced; compared with the model group, the degree of gastric pathological damage in the Haiwang Jinzun group was significantly reduced.

[0091] The results of Example 3 show that Huoxiang Anwei Powder has a significant protective effect on alcohol-induced acute drunkenness and related liver and stomach damage at high and medium doses. This protective effect may be related to Huoxiang Anwei Powder's ability to enhance ethanol metabolism, improve antioxidant capacity, reduce lipid peroxidation, and inhibit inflammatory response. At the same time, the intervention of the Haiwang Jinzun group also showed a certain protective effect, but in comparison, Huoxiang Anwei Powder performed better in many indicators.

[0092] Comparative Example 1

[0093] In the reference "Jiang Yichuan, et al. Protective effect of ginseng and ganoderma lucidum ultrafine powder on chronic liver injury in rats induced by alcohol [J]. Ginseng Research, 2019, 31(01): 2~4.", except for the normal group which was given distilled water, the other groups were gavaged with 56% liquor, 8mL / kg in the first week, and then increased by 1mL every week for 8 consecutive weeks to establish a rat model of chronic alcoholic liver injury. At the same time, ginseng and ganoderma lucidum ultrafine powder were gavaged to observe the changes in liver index, liver function, lipid peroxides and cellular inflammatory factors in rats with chronic alcoholic liver injury. Compared with the model group, the high-dose group of ginseng ultrafine powder (0.5g / kg) can significantly increase the body weight of rats, reduce liver index, reduce serum TNF-α, L6 content and AST, ALT activity, and significantly reduce liver tissue MDA and TG content, increase GSH content and SOD activity ( P <0.05 or P <0.01).

[0094] In comparative example 1, the dosage of the high-dose group of ginseng superfine powder was 0.5g / kg, while the proportion of ginseng in the Huoxiang Anwei Powder prescription of Example 1 was 1 / 5, and the high-dose mouse dosage was 140mg / kg, which was equivalent to a ginseng dosage of 28mg / kg. In the study of the reference, there was a detection of the same indicator-liver GSH. The liver GSH of the chronic liver injury model decreased by 30% compared with the control group, and the high-dose ginseng superfine powder increased by 30% compared with the model group (from 4.06±0.67mg / g in the control group to 5.24±0.35 mg / g in the ginseng intervention group). In this experiment, the liver GSH of the acute liver injury model decreased by 50% compared with the control group, and the high-dose Huoxiang Anwei Powder increased by 60% compared with the model group (from 2895.33±198.36μmol / g to 4769.21±343.66μmol / g in the Huoxiang Anwei Powder intervention group). This comparison shows that Huoxiang Anwei San can restore liver GSH more and faster in a more severe acute liver injury model at a dose of 1 / 25 of ginseng.

[0095] It can be seen that the single herb ginseng in Comparative Example 1 only showed the effects of sobering up and protecting the liver at a dose equivalent to 6 times the high dose of Huoxiang Anwei Powder in Example 1. This shows that the formulation of Huoxiang Anwei Powder brings a higher dose efficiency. Multi-component herbal preparations usually have synergistic effects, which may play a role at multiple levels and provide more comprehensive protection. It is particularly worth noting that due to the optimization of the extraction process, even if the same amount of raw drugs is required to exert the efficacy, the dosage of Huoxiang Anwei Powder is much smaller than that of most Chinese medicine extracts or powders. Such advantages can improve patients' medication compliance, reduce dose-dependent side effects, facilitate preparation, improve bioavailability, and facilitate dosage adjustment.

[0096] Therefore, the research value of Huoxiang Anwei San as a potential drug against alcoholic liver and stomach damage lies in its potential to sober up and protect the liver in animal models, which provides a scientific basis for in-depth exploration of its drug efficacy, dose-effect relationship, and mechanism of action. Optimized extraction process may bring better safety, economy, and environmental sustainability.

[0097] Although the above embodiment describes the present invention in detail, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Use of Huoxiang Anwei Powder in the preparation of a drug for preventing and / or treating alcohol-induced liver and stomach damage; the preparation method of Huoxiang Anwei Powder comprises the following steps: Weigh patchouli, clove, ginseng and tangerine peel in a mass ratio of 1:1:1:2, and reflux to separate to obtain volatile oil and water extract; The water extract is concentrated and freeze-dried to obtain freeze-dried powder; the volatile oil and the freeze-dried powder are mixed to obtain Huoxiang Anwei Powder.

2. The use according to claim 1, characterized in that: Calculated based on the weight of mice, the effective dose of the Huoxiang Anwei Powder is 35-160 mg / kg.

3. The use according to claim 1, characterized in that: The efficacy of the drug includes at least one of the following: 1) Reduce the concentration of ethanol in the blood; 2) Prevent and treat liver damage caused by ethanol; 3) Prevent and treat gastric function damage caused by ethanol; 4) Enhance the activity of acetaldehyde dehydrogenase in the liver.

4. The use according to claim 3, characterized in that: The biochemical indicators of liver function damage include: triglyceride content and / or glutathione.

5. The use according to claim 3, characterized in that: The biochemical indexes of gastric function damage include: catalase activity.

6. The use according to claim 1, characterized in that: The dosage form of the Huoxiang Anwei Powder includes at least one of the following: powder, capsule, tablet, decoction, pill, paste and granule.

7. The use according to any one of claims 1 to 6, characterized in that: The Huoxiang Anwei Powder comprises the following raw materials in parts by weight: 40-60 parts of patchouli, 40-60 parts of cloves, 40-60 parts of ginseng and 80-120 parts of tangerine peel.

8. Application of Huoxiang Anwei Powder in the preparation of health food with auxiliary protective effect on alcoholic liver damage; The preparation method of Huoxiang Anwei Powder comprises the following steps: Weigh patchouli, clove, ginseng and tangerine peel in a mass ratio of 1:1:1:2, and reflux to separate to obtain volatile oil and water extract; The water extract is concentrated and freeze-dried to obtain freeze-dried powder; the volatile oil and the freeze-dried powder are mixed to obtain Huoxiang Anwei Powder.

9. Application of Huoxiang Anwei Powder in the preparation of hangover medicine; the preparation method of Huoxiang Anwei Powder comprises the following steps: Weigh patchouli, clove, ginseng and tangerine peel in a mass ratio of 1:1:1:2, and reflux to separate to obtain volatile oil and water extract; The water extract is concentrated and freeze-dried to obtain freeze-dried powder; the volatile oil and the freeze-dried powder are mixed to obtain Huoxiang Anwei Powder.

10. Application of Huoxiang Anwei Powder in the preparation of alcohol-relieving drugs; the preparation method of Huoxiang Anwei Powder comprises the following steps: Weigh patchouli, clove, ginseng and tangerine peel in a mass ratio of 1:1:1:2, and reflux to separate to obtain volatile oil and water extract; The water extract is concentrated and freeze-dried to obtain freeze-dried powder; the volatile oil and the freeze-dried powder are mixed to obtain Huoxiang Anwei Powder.

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